Rw5G035590

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005691
Physical Location & Seq
Reverse (-)
61888333 .. 61891750
3418 bp
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UTR
Exon/CDS
Intron
Rw5G035590.1

Sequence Viewer

Length: 381 bp
ATGAGTCTTATGAAGCGGTATGTGCTGCGGTTGTTCATATCATTGAAGTACATCACAGCAAACGTCGTTGACAGAAATAATGGCCGGATTGTTGTAACAGCATCTACAATTGAACATTCAGTTAAGGGCTCGCTCGAGTGTGGCCGCTCTTGCAACGCAAAGGCGGCAGCAGTTGTTGGAGAGGTGCTCGCTATGCGACTTAAGGTGGAAGGTCTTGACCAGGGACAGGGACGAGGGATTCATGTCAATGTAAATAATGAAGTTGAGAAGAAGGGTTTTAAAAACCGTACCAAGGTATGGGCTATTGTCAATGCTCTTAAAAACAATGGAGTTAAACTCATTCTTGATGATAATGATGAAAATAGTTCTCAGCCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

126

Amino Acids

13.82

Weight (kDa)

9.69

Isoelectric Point (pI)

21.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 5 - 74 4.9e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 297
AccBSI CCGCTC 1 cut(s) 147
AciI CCGC 4 cut(s) 16, 28, 145, 164
AcoI YGGCCR 2 cut(s) 82, 142
AfaI GTAC 2 cut(s) 50, 289
AfiI CCNNNNNNNGG 3 cut(s) 226, 292, 297
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 2 cut(s) 46, 113
AjnI CCWGG 1 cut(s) 219
Alw21I GWGCWC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 2 cut(s) 82, 142
ApeKI GCWGC 2 cut(s) 25, 167
AvaI CYCGRG 1 cut(s) 134
BanII GRGCYC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 12, 179
BciT130I CCWGG 1 cut(s) 221
BfrI CTTAAG 1 cut(s) 200
BisI GCNGC 4 cut(s) 26, 145, 165, 168
BlsI GCNGC 4 cut(s) 27, 146, 166, 169
Bme1390I CCNGG 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 1 cut(s) 110
BplI GAGNNNNNCTC 4 cut(s) 171, 203, 321, 353
BsaJI CCNNGG 2 cut(s) 220, 291
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 292, 297
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 220, 291
BseLI CCNNNNNNNGG 3 cut(s) 226, 292, 297
BseXI GCAGC 2 cut(s) 12, 179
BshFI GGCC 2 cut(s) 84, 144
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 237, 243
BslI CCNNNNNNNGG 3 cut(s) 226, 292, 297
BsmFI GGGAC 2 cut(s) 237, 243
BsnI GGCC 2 cut(s) 84, 144
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 131, 189
BspACI CCGC 4 cut(s) 16, 28, 145, 164
BspANI GGCC 2 cut(s) 84, 144
BspTI CTTAAG 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 147
BssECI CCNNGG 2 cut(s) 220, 291
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 287
BstAFI CTTAAG 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 131, 189
BstDEI CTNAG 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 22, 150, 164, 193
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 12, 179
BsuRI GGCC 2 cut(s) 84, 144
Cac8I GCNNGC 2 cut(s) 131, 189
Csp6I GTAC 2 cut(s) 49, 288
CviAII CATG 1 cut(s) 242
CviJI RGCY 5 cut(s) 84, 129, 144, 302, 373
CviKI_1 RGCY 5 cut(s) 84, 129, 144, 302, 373
CviQI GTAC 2 cut(s) 49, 288
DdeI CTNAG 1 cut(s) 369
DraI TTTAAA 1 cut(s) 280
EaeI YGGCCR 2 cut(s) 82, 142
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 1 cut(s) 245
FaiI YATR 6 cut(s) 11, 21, 38, 194, 243, 298
FaqI GGGAC 2 cut(s) 237, 243
FatI CATG 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 26, 145, 165, 168
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 4 cut(s) 26, 145, 165, 168
GluI GCNGC 4 cut(s) 26, 145, 165, 168
HaeIII GGCC 2 cut(s) 84, 144
HapII CCGG 1 cut(s) 85
Hin1II CATG 1 cut(s) 245
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 2 cut(s) 4, 238
HpaII CCGG 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 70
Hpy188III TCNNGA 2 cut(s) 215, 344
Hpy8I GTNNAC 1 cut(s) 70
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 203, 265
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 153
HpyF10VI GCNNNNNNNGC 4 cut(s) 22, 150, 164, 193
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 1 cut(s) 245
LpnPI CCDG 4 cut(s) 98, 206, 212, 233
Lsp1109I GCAGC 2 cut(s) 12, 179
LweI GCATC 1 cut(s) 110
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 94
MbiI CCGCTC 1 cut(s) 147
MboII GAAGA 1 cut(s) 280
MfeI CAATTG 1 cut(s) 108
MhlI GDGCHC 2 cut(s) 131, 189
MluCI AATT 1 cut(s) 108
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 157
MnlI CCTC 2 cut(s) 175, 227
MseI TTAA 6 cut(s) 123, 201, 279, 318, 333, 379
MslI CAYNNNNRTG 1 cut(s) 246
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 221
MunI CAATTG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 22, 150, 164, 193
NlaIII CATG 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 134
PfeI GAWTC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 297
PkrI GCNGC 4 cut(s) 27, 146, 166, 169
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspXI VCTCGAGB 1 cut(s) 134
RsaI GTAC 2 cut(s) 50, 289
RsaNI GTAC 2 cut(s) 49, 288
RseI CAYNNNNRTG 1 cut(s) 246
SaqAI TTAA 6 cut(s) 123, 201, 279, 318, 333, 379
SatI GCNGC 4 cut(s) 26, 145, 165, 168
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 2 cut(s) 131, 189
SetI ASST 5 cut(s) 66, 186, 207, 214, 297
SfaNI GCATC 1 cut(s) 110
Sfr274I CTCGAG 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 2 cut(s) 134, 200
SmoI CTYRAG 2 cut(s) 134, 200
Sse9I AATT 1 cut(s) 108
SsiI CCGC 4 cut(s) 16, 28, 145, 164
StyD4I CCNGG 1 cut(s) 219
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 108
TatI WGTACW 1 cut(s) 48
TauI GCSGC 2 cut(s) 147, 167
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 6 cut(s) 123, 201, 279, 318, 333, 379
Tru9I TTAA 6 cut(s) 123, 201, 279, 318, 333, 379
TseI GCWGC 2 cut(s) 25, 167
TspDTI ATGAA 5 cut(s) 25, 26, 230, 273, 372
Van91I CCANNNNNTGG 1 cut(s) 297
Vha464I CTTAAG 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.