Rh5CG412900

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005691
Physical Location & Seq
Reverse (-)
55865784 .. 55868133
2350 bp
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UTR
Exon/CDS
Intron
Rh5CG412900.1

Sequence Viewer

Length: 471 bp
ATGCCTGACTGTTGGTATTTGCAATTTCTTTCATTTGGTTCATATCTAATTGGCTTTTTGTTAAACACAAAACTCCAGGAGTGTCCAAGAATGAGTCTTATGAAGCGGTATGTGCTGCGGTTGTTCATATCATTGAAGTACATCACAGCAAACGTCGTTGACAGAAATAATGGCCGGATTGTTGTAACAGCATCTACAATTGAACATTCAGTTAAGGGCTCGCTCGAGTGTGGCCGCTCTTGCAACGCAAAGGCGGCAGCAGTTGTTGGAGAGGTGCTCGCTATGCGACTTAAGGTGGAAGGTCTTGACCAGGGACAGGGACGAGGGATTCATGTCAATGTTAATAATGAAGTTGAGAAGAAGGGTTTTAAGAACCGTACCAAGGTATGGGCTATTGTCAATGCTCTTAAAAACAATGGAGTTAAACTCATTCTTGATGATAATGATGAAAATAGTTCTCAGCCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

156

Amino Acids

17.39

Weight (kDa)

9.39

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 35 - 104 7.6e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 387
AccBSI CCGCTC 1 cut(s) 237
AciI CCGC 4 cut(s) 106, 118, 235, 254
AcoI YGGCCR 2 cut(s) 172, 232
AfaI GTAC 2 cut(s) 140, 379
AfiI CCNNNNNNNGG 3 cut(s) 316, 382, 387
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 2 cut(s) 136, 203
AjnI CCWGG 2 cut(s) 75, 309
Alw21I GWGCWC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 224
AoxI GGCC 2 cut(s) 172, 232
ApeKI GCWGC 2 cut(s) 115, 257
AvaI CYCGRG 1 cut(s) 224
BanII GRGCYC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 102, 269
BciT130I CCWGG 2 cut(s) 77, 311
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 4 cut(s) 116, 235, 255, 258
BlsI GCNGC 4 cut(s) 117, 236, 256, 259
Bme1390I CCNGG 2 cut(s) 77, 311
BmeT110I CYCGRG 1 cut(s) 224
BmrFI CCNGG 2 cut(s) 77, 311
BmsI GCATC 1 cut(s) 200
BplI GAGNNNNNCTC 4 cut(s) 261, 293, 411, 443
BpmI CTGGAG 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 310, 381
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 382, 387
BseBI CCWGG 2 cut(s) 77, 311
BseDI CCNNGG 2 cut(s) 310, 381
BseLI CCNNNNNNNGG 3 cut(s) 316, 382, 387
BseXI GCAGC 2 cut(s) 102, 269
BshFI GGCC 2 cut(s) 174, 234
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 1 cut(s) 175
BslFI GGGAC 2 cut(s) 327, 333
BslI CCNNNNNNNGG 3 cut(s) 316, 382, 387
BsmFI GGGAC 2 cut(s) 327, 333
BsnI GGCC 2 cut(s) 174, 234
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 221, 279
BspACI CCGC 4 cut(s) 106, 118, 235, 254
BspANI GGCC 2 cut(s) 174, 234
BspTI CTTAAG 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 310, 381
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 2 cut(s) 77, 311
Bst4CI ACNGT 2 cut(s) 11, 377
BstAFI CTTAAG 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 221, 279
BstDEI CTNAG 1 cut(s) 459
BstMWI GCNNNNNNNGC 4 cut(s) 112, 240, 254, 283
BstNI CCWGG 2 cut(s) 77, 311
BstSCI CCNGG 2 cut(s) 75, 309
BstV1I GCAGC 2 cut(s) 102, 269
BsuRI GGCC 2 cut(s) 174, 234
Cac8I GCNNGC 2 cut(s) 221, 279
Csp6I GTAC 2 cut(s) 139, 378
CviAII CATG 1 cut(s) 332
CviJI RGCY 6 cut(s) 54, 174, 219, 234, 392, 463
CviKI_1 RGCY 6 cut(s) 54, 174, 219, 234, 392, 463
CviQI GTAC 2 cut(s) 139, 378
DdeI CTNAG 1 cut(s) 459
EaeI YGGCCR 2 cut(s) 172, 232
Eco130I CCWWGG 1 cut(s) 381
Eco24I GRGCYC 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 75, 309
EcoT14I CCWWGG 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 1 cut(s) 335
FaiI YATR 7 cut(s) 43, 101, 111, 128, 284, 333, 388
FaqI GGGAC 2 cut(s) 327, 333
FatI CATG 1 cut(s) 331
Fnu4HI GCNGC 4 cut(s) 116, 235, 255, 258
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 4 cut(s) 116, 235, 255, 258
GluI GCNGC 4 cut(s) 116, 235, 255, 258
GsuI CTGGAG 1 cut(s) 59
HaeIII GGCC 2 cut(s) 174, 234
HapII CCGG 1 cut(s) 175
Hin1II CATG 1 cut(s) 335
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 94, 328
HpaII CCGG 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 160
Hpy188III TCNNGA 2 cut(s) 305, 434
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 293, 355
HpyCH4III ACNGT 2 cut(s) 11, 377
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 22, 243
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 240, 254, 283
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 1 cut(s) 335
LpnPI CCDG 7 cut(s) 18, 62, 89, 188, 296, 302, 323
Lsp1109I GCAGC 2 cut(s) 102, 269
LweI GCATC 1 cut(s) 200
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 184
MbiI CCGCTC 1 cut(s) 237
MboII GAAGA 1 cut(s) 370
MfeI CAATTG 1 cut(s) 198
MhlI GDGCHC 2 cut(s) 221, 279
MluCI AATT 3 cut(s) 23, 48, 198
MlyI GAGTC 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 2 cut(s) 265, 317
MseI TTAA 8 cut(s) 62, 213, 291, 342, 369, 408, 423, 469
MslI CAYNNNNRTG 1 cut(s) 336
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 1 cut(s) 175
MspR9I CCNGG 2 cut(s) 77, 311
MunI CAATTG 1 cut(s) 198
MvaI CCWGG 2 cut(s) 77, 311
MwoI GCNNNNNNNGC 4 cut(s) 112, 240, 254, 283
NlaIII CATG 1 cut(s) 335
PaeR7I CTCGAG 1 cut(s) 224
PfeI GAWTC 1 cut(s) 328
PflMI CCANNNNNTGG 1 cut(s) 387
PfoI TCCNGGA 1 cut(s) 75
PkrI GCNGC 4 cut(s) 117, 236, 256, 259
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 75, 309
PspGI CCWGG 2 cut(s) 75, 309
PspXI VCTCGAGB 1 cut(s) 224
RsaI GTAC 2 cut(s) 140, 379
RsaNI GTAC 2 cut(s) 139, 378
RseI CAYNNNNRTG 1 cut(s) 336
SaqAI TTAA 8 cut(s) 62, 213, 291, 342, 369, 408, 423, 469
SatI GCNGC 4 cut(s) 116, 235, 255, 258
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 2 cut(s) 77, 311
SduI GDGCHC 2 cut(s) 221, 279
SetI ASST 5 cut(s) 156, 276, 297, 304, 387
SfaNI GCATC 1 cut(s) 200
Sfr274I CTCGAG 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 2 cut(s) 224, 290
SmoI CTYRAG 2 cut(s) 224, 290
Sse9I AATT 3 cut(s) 23, 48, 198
SsiI CCGC 4 cut(s) 106, 118, 235, 254
StyD4I CCNGG 2 cut(s) 75, 309
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 11, 377
TaiI ACGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 225
TasI AATT 3 cut(s) 23, 48, 198
TatI WGTACW 1 cut(s) 138
TauI GCSGC 2 cut(s) 237, 257
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 8 cut(s) 62, 213, 291, 342, 369, 408, 423, 469
Tru9I TTAA 8 cut(s) 62, 213, 291, 342, 369, 408, 423, 469
TseI GCWGC 2 cut(s) 115, 257
TspDTI ATGAA 7 cut(s) 21, 30, 115, 116, 320, 363, 462
Van91I CCANNNNNTGG 1 cut(s) 387
Vha464I CTTAAG 1 cut(s) 290
XhoI CTCGAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.