FvH4_3g32900

Isovaleryl-CoA dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006675
Physical Location & Seq
Reverse (-)
28293426 .. 28299238
5813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32900.t6

Sequence Viewer

Length: 1227 bp
ATGCAAAGACTGGTTTCAGCGAGGTCTGGGCTCACAGGTCTTCTCAGAAACCAGAGACACTCTGCGTCGTTCTCCACCGCTCTACTCTTCGATGACACTCAGATTCAGTTTAAGGAAAGTGTGGGTCAGTTTGCACAAGAACACATTGCCCCACATGCTTCAAAAATAGACCACACCAATAATTTTCCCAAGGAGGTCAACTTGTGGAAGCTTATGGGGGAGTTTAATCTTCATGGAATTACTGCCCCAGAGGAATATGGAGGCTTGGGTCTTGGCTATTTGTATCACTGCATAGCTATGGAAGAAATAAGCCGGGCTTCAGGATCTGTCGGTCTATCTTATGGTGCCCATTCCAACCTGTGCATTAATCAATTGGTGAGGCATGGAAATCCTACTCAGAAAGAGAAGTATTTGCCAAAGCTTATAAGTGGAAAGCATGTAGGGGCCCTTGCAATGAGTGAGCCCAATTCTGGTTCTGATGTTGTTAGCATGAAGTGCAAAGCTGATCGAGCAGATGGGGGTTATATCTTAAATGGGAACAAGATGTGGTGCACCAATGGCCCAGTTGCTCAAACATTGGTTGTTTACGCAAAAACGGACATAACTGCGGGCTCAAAGGGAATAACAGCATTCATCATTGAGAAGGGGATGCCCGGGTTCAGTACTGCCCAAAAATTAGACAAACTCGGCATGCGAGGAAGCGATACATGTGAGCTTGTCTTCGAGAATTGCTTTGTTCCAGATGAAAATGTTCTTGGTCAGGAAGGAAAAGGAGTCTATGTCATGATGTCAGGGTTGGATTTAGAGAGGCTTGTGTTAGCAGGTGGGCCCCTAGGTATTATGCAGGCATGTATGGATGTTATCCTTCCATATGTTCGACAGCGAGAGCAGTTTGGCCGTCCGATTGGAGAGTTTCAGCTTATACAGGGGAAAATTGCTGACATGTATACTTCTTTACAATCTTCAAGGGCCTATGTGTATTCTGTTGCAAGGGAATGTGACAATGGAAGAGTTGACCCAAAGGACTGTGCTGGAGTTATACTATCTACGGCTGAAAGAGCCACGCAGGTTGCTTTGCAGGCTATACAATGCTTAGGTGGTAATGGCTATGTAAATGAGTACTCAACTGGCCGTCTCCTTCGAGATGCCAAACTCTATGAGATTGGAGCAGGGACCAGTGAGATCAGAAGAATGATCATTGGTCGTGGACTATTTAAGGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0003853 GO:0003995 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006139 GO:0006163 GO:0006195 GO:0006520 GO:0006551 GO:0006552 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008470 GO:0009056 GO:0009058 GO:0009063 GO:0009081 GO:0009083 GO:0009109 GO:0009117 GO:0009150 GO:0009154 GO:0009166 GO:0009259 GO:0009261 GO:0009987 GO:0016054 GO:0016491 GO:0016627 GO:0017076 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033869 GO:0033875 GO:0034031 GO:0034032 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035337 GO:0035383 GO:0035639 GO:0036094 GO:0036115 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046434 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0072521 GO:0072523 GO:0097159 GO:0097367 GO:1901135 GO:1901136 GO:1901265 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901568 GO:1901569 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1902189 GO:1902190 GO:1902192 GO:1902195 GO:1902196 GO:1902198
KEGG Pathways
Metabolic & Signaling
ko00280 ko01100 map00280 map01100
Pfam Domains
Protein Families
Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

409

Amino Acids

44.47

Weight (kDa)

7.55

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-CoA_dh_N PF02771 32 - 145 8.2e-39 Acyl-CoA dehydrogenase, N-terminal domain
Acyl-CoA_dh_M PF02770 149 - 244 7.4e-26 Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1 PF00441 256 - 310 7.8e-14 Acyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AarI CACCTGC 1 cut(s) 812
Acc36I ACCTGC 2 cut(s) 812, 1057
AccB1I GGYRCC 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 947
AciI CCGC 2 cut(s) 78, 608
AclWI GGATC 1 cut(s) 331
AcoI YGGCCR 2 cut(s) 895, 1129
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 2 cut(s) 664, 1121
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 2 cut(s) 707, 942
AgsI TTSAA 2 cut(s) 162, 966
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
AluBI AGCT 6 cut(s) 211, 296, 421, 503, 715, 919
AluI AGCT 6 cut(s) 211, 296, 421, 503, 715, 919
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 49, 1139
Alw44I GTGCAC 1 cut(s) 550
AlwI GGATC 1 cut(s) 331
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 1 cut(s) 653
AoxI GGCC 6 cut(s) 444, 559, 827, 895, 969, 1129
ApaI GGGCCC 2 cut(s) 448, 831
ApaLI GTGCAC 1 cut(s) 550
AseI ATTAAT 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 750
AspA2I CCTAGG 1 cut(s) 832
AspS9I GGNCC 7 cut(s) 444, 445, 560, 827, 828, 969, 1173
AsuC2I CCSGG 3 cut(s) 314, 654, 655
AsuHPI GGTGA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 1 cut(s) 1173
AvrII CCTAGG 1 cut(s) 832
BaeGI GKGCMC 4 cut(s) 349, 448, 554, 831
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 5 cut(s) 33, 448, 465, 614, 831
BbsI GAAGAC 2 cut(s) 32, 712
Bbv12I GWGCWC 1 cut(s) 554
BccI CCATC 1 cut(s) 509
BceAI ACGGC 3 cut(s) 882, 1065, 1116
BclI TGATCA 1 cut(s) 1194
BcnI CCSGG 3 cut(s) 314, 654, 655
BcoDI GTCTC 2 cut(s) 49, 1139
BfaI CTAG 1 cut(s) 833
BfuAI ACCTGC 2 cut(s) 812, 1057
BlnI CCTAGG 1 cut(s) 832
BmcAI AGTACT 2 cut(s) 664, 1121
Bme1390I CCNGG 3 cut(s) 314, 654, 655
Bme18I GGWCC 1 cut(s) 1173
BmeT110I CYCGRG 1 cut(s) 653
BmgT120I GGNCC 7 cut(s) 444, 445, 560, 827, 828, 969, 1173
BmiI GGNNCC 6 cut(s) 346, 445, 446, 829, 830, 1174
BmrFI CCNGG 3 cut(s) 314, 654, 655
BmrI ACTGGG 1 cut(s) 557
BmsI GCATC 2 cut(s) 639, 1135
BmuI ACTGGG 1 cut(s) 557
BpiI GAAGAC 2 cut(s) 32, 712
BpmI CTGGAG 1 cut(s) 1053
Bpu10I CCTNAGC 1 cut(s) 1093
BpuMI CCSGG 3 cut(s) 314, 654, 655
BsaJI CCNNGG 3 cut(s) 189, 653, 832
BsaXI ACNNNNNCTCC 2 cut(s) 765, 795
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 4 cut(s) 15, 563, 1132, 1176
Bse3DI GCAATG 2 cut(s) 144, 459
BseDI CCNNGG 3 cut(s) 189, 653, 832
BseGI GGATG 2 cut(s) 654, 862
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 2 cut(s) 144, 459
BseMII CTCAG 3 cut(s) 58, 113, 410
BseNI ACTGG 4 cut(s) 15, 563, 1132, 1176
BseSI GKGCMC 4 cut(s) 349, 448, 554, 831
BshFI GGCC 6 cut(s) 446, 561, 829, 897, 971, 1131
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 2 cut(s) 313, 654
BslFI GGGAC 1 cut(s) 1186
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 49, 1139
BsmBI CGTCTC 1 cut(s) 1139
BsmFI GGGAC 1 cut(s) 1186
BsmI GAATGC 1 cut(s) 629
BsnI GGCC 6 cut(s) 446, 561, 829, 897, 971, 1131
BsoBI CYCGRG 1 cut(s) 653
Bsp120I GGGCCC 2 cut(s) 444, 827
Bsp1286I GDGCHC 7 cut(s) 33, 349, 448, 465, 554, 614, 831
Bsp143I GATC 4 cut(s) 323, 505, 1182, 1194
BspACI CCGC 2 cut(s) 78, 608
BspANI GGCC 6 cut(s) 446, 561, 829, 897, 971, 1131
BspCNI CTCAG 3 cut(s) 57, 112, 409
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 6 cut(s) 346, 445, 446, 829, 830, 1174
BspMI ACCTGC 2 cut(s) 812, 1057
BspPI GGATC 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 344
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 144, 459
BsrI ACTGG 4 cut(s) 15, 563, 1132, 1176
BssECI CCNNGG 3 cut(s) 189, 653, 832
BssMI GATC 4 cut(s) 323, 505, 1182, 1194
BssNAI GTATAC 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 189, 832
Bst1107I GTATAC 1 cut(s) 948
Bst4CI ACNGT 1 cut(s) 1028
Bst6I CTCTTC 2 cut(s) 92, 1003
BstAPI GCANNNNNTGC 1 cut(s) 495
BstC8I GCNNGC 4 cut(s) 610, 692, 846, 1080
BstDEI CTNAG 4 cut(s) 44, 99, 396, 1093
BstF5I GGATG 2 cut(s) 654, 862
BstKTI GATC 4 cut(s) 326, 508, 1185, 1197
BstMAI GTCTC 2 cut(s) 49, 1139
BstMBI GATC 4 cut(s) 323, 505, 1182, 1194
BstMWI GCNNNNNNNGC 6 cut(s) 155, 495, 509, 558, 1058, 1079
BstNSI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
BstSCI CCNGG 3 cut(s) 312, 652, 653
BstSLI GKGCMC 4 cut(s) 349, 448, 554, 831
BstV2I GAAGAC 2 cut(s) 32, 712
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BstZ17I GTATAC 1 cut(s) 948
BsuRI GGCC 6 cut(s) 446, 561, 829, 897, 971, 1131
BtsCI GGATG 2 cut(s) 654, 862
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 2 cut(s) 286, 1183
BveI ACCTGC 2 cut(s) 812, 1057
Cac8I GCNNGC 4 cut(s) 610, 692, 846, 1080
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 783
Cfr13I GGNCC 7 cut(s) 444, 445, 560, 827, 828, 969, 1173
Cfr9I CCCGGG 1 cut(s) 653
CseI GACGC 1 cut(s) 54
Csp6I GTAC 2 cut(s) 663, 1120
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 2 cut(s) 663, 1120
DdeI CTNAG 4 cut(s) 44, 99, 396, 1093
DpnI GATC 4 cut(s) 325, 507, 1184, 1196
DpnII GATC 4 cut(s) 323, 505, 1182, 1194
EaeI YGGCCR 2 cut(s) 895, 1129
Eam1104I CTCTTC 2 cut(s) 92, 1003
EarI CTCTTC 2 cut(s) 92, 1003
Eco130I CCWWGG 2 cut(s) 189, 832
Eco24I GRGCYC 5 cut(s) 33, 448, 465, 614, 831
Eco47I GGWCC 1 cut(s) 1173
Eco57I CTGAAG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 653
EcoO109I RGGNCCY 4 cut(s) 444, 445, 828, 969
EcoT14I CCWWGG 2 cut(s) 189, 832
EcoT38I GRGCYC 5 cut(s) 33, 448, 465, 614, 831
ErhI CCWWGG 2 cut(s) 189, 832
Esp3I CGTCTC 1 cut(s) 1139
FalI AAGNNNNNCTT 2 cut(s) 301, 333
FaqI GGGAC 1 cut(s) 1186
FauI CCCGC 1 cut(s) 601
FauNDI CATATG 1 cut(s) 871
FbaI TGATCA 1 cut(s) 1194
FblI GTMKAC 1 cut(s) 947
FokI GGATG 2 cut(s) 661, 869
FriOI GRGCYC 5 cut(s) 33, 448, 465, 614, 831
FspBI CTAG 1 cut(s) 833
GsuI CTGGAG 1 cut(s) 1053
HaeIII GGCC 6 cut(s) 446, 561, 829, 897, 971, 1131
HapII CCGG 2 cut(s) 313, 654
HgaI GACGC 1 cut(s) 54
HincII GTYRAC 2 cut(s) 199, 1015
HindII GTYRAC 2 cut(s) 199, 1015
HindIII AAGCTT 2 cut(s) 209, 419
HinfI GANTC 2 cut(s) 103, 774
HpaII CCGG 2 cut(s) 313, 654
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 6 cut(s) 199, 552, 586, 948, 1015, 1208
Hpy188I TCNGA 6 cut(s) 47, 102, 399, 478, 903, 1187
Hpy188III TCNNGA 6 cut(s) 321, 724, 740, 761, 784, 1142
Hpy8I GTNNAC 6 cut(s) 199, 552, 586, 948, 1015, 1208
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 4 cut(s) 637, 758, 875, 1148
HpyCH4III ACNGT 1 cut(s) 1028
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 495, 509, 558, 1058, 1079
HpyF3I CTNAG 4 cut(s) 44, 99, 396, 1093
Ksp22I TGATCA 1 cut(s) 1194
Kzo9I GATC 4 cut(s) 323, 505, 1182, 1194
LmnI GCTCC 2 cut(s) 1166, 1219
LweI GCATC 2 cut(s) 639, 1135
MaeI CTAG 1 cut(s) 833
MaeIII GTNAC 1 cut(s) 998
MalI GATC 4 cut(s) 325, 507, 1184, 1196
MbiI CCGCTC 1 cut(s) 80
MboI GATC 4 cut(s) 323, 505, 1182, 1194
MboII GAAGA 8 cut(s) 32, 79, 221, 314, 712, 954, 1020, 1200
MfeI CAATTG 1 cut(s) 371
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 7 cut(s) 33, 349, 448, 465, 554, 614, 831
MluCI AATT 7 cut(s) 181, 237, 371, 466, 674, 727, 933
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 2 cut(s) 378, 777
MnlI CCTC 7 cut(s) 15, 187, 244, 254, 372, 689, 801
MroXI GAANNNNTTC 1 cut(s) 750
MseI TTAA 5 cut(s) 111, 225, 366, 530, 1215
MslI CAYNNNNRTG 1 cut(s) 296
MspI CCGG 2 cut(s) 313, 654
MspR9I CCNGG 3 cut(s) 314, 654, 655
MunI CAATTG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 629
MwoI GCNNNNNNNGC 6 cut(s) 155, 495, 509, 558, 1058, 1079
NciI CCSGG 3 cut(s) 314, 654, 655
NdeI CATATG 1 cut(s) 871
NdeII GATC 4 cut(s) 323, 505, 1182, 1194
NlaIV GGNNCC 6 cut(s) 346, 445, 446, 829, 830, 1174
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 998
NspI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
PaeI GCATGC 1 cut(s) 694
PagI TCATGA 1 cut(s) 783
PaqCI CACCTGC 1 cut(s) 812
PciI ACATGT 2 cut(s) 707, 942
PctI GAATGC 1 cut(s) 629
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 103
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
PscI ACATGT 2 cut(s) 707, 942
PshBI ATTAAT 1 cut(s) 366
PsiI TTATAA 1 cut(s) 425
PspN4I GGNNCC 6 cut(s) 346, 445, 446, 829, 830, 1174
PspOMI GGGCCC 2 cut(s) 444, 827
PspPI GGNCC 7 cut(s) 444, 445, 560, 827, 828, 969, 1173
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 2 cut(s) 664, 1121
RsaNI GTAC 2 cut(s) 663, 1120
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 5 cut(s) 111, 225, 366, 530, 1215
Sau3AI GATC 4 cut(s) 323, 505, 1182, 1194
Sau96I GGNCC 7 cut(s) 444, 445, 560, 827, 828, 969, 1173
ScaI AGTACT 2 cut(s) 664, 1121
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 3 cut(s) 314, 654, 655
SduI GDGCHC 7 cut(s) 33, 349, 448, 465, 554, 614, 831
SfaNI GCATC 2 cut(s) 639, 1135
SinI GGWCC 1 cut(s) 1173
SmaI CCCGGG 1 cut(s) 655
SmiMI CAYNNNNRTG 1 cut(s) 296
SphI GCATGC 1 cut(s) 694
Sse9I AATT 7 cut(s) 181, 237, 371, 466, 674, 727, 933
SsiI CCGC 2 cut(s) 78, 608
SspMI CTAG 1 cut(s) 833
StyD4I CCNGG 3 cut(s) 312, 652, 653
StyI CCWWGG 2 cut(s) 189, 832
TaaI ACNGT 1 cut(s) 1028
TaqI TCGA 5 cut(s) 90, 508, 723, 877, 1141
TaqII GACCGA 1 cut(s) 320
TasI AATT 7 cut(s) 181, 237, 371, 466, 674, 727, 933
TatI WGTACW 2 cut(s) 662, 1119
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 5 cut(s) 111, 225, 366, 530, 1215
Tru9I TTAA 5 cut(s) 111, 225, 366, 530, 1215
TscAI CASTG 2 cut(s) 293, 1183
TseFI GTSAC 1 cut(s) 998
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 4 cut(s) 221, 506, 622, 759
TspGWI ACGGA 1 cut(s) 611
TspMI CCCGGG 1 cut(s) 653
TspRI CASTG 2 cut(s) 293, 1183
VneI GTGCAC 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 1173
VspI ATTAAT 1 cut(s) 366
XceI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
XmaI CCCGGG 1 cut(s) 653
XmaJI CCTAGG 1 cut(s) 832
XmiI GTMKAC 1 cut(s) 947
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 1 cut(s) 833
ZrmI AGTACT 2 cut(s) 664, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.