RLG00000035340

Isovaleryl-CoA dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006675
Physical Location & Seq
Reverse (-)
65337721 .. 65342808
5088 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035340

Sequence Viewer

Length: 1227 bp
ATGCAAAGACTGGTTTCAGCGAGGTCTGGGATTACAAGTCTTCTCAGAAATCAGAGACACGCGGCTTCTTTCTCTACCTCTTTACTCTTCGATGATACTCAAATTCAGTTTAAGGAAAGTGTGGGTCAATTTGCACAAGAACACATTGCCCCACATGCTTCAAAAGTAGACCAAACTAATAATTTTCCCAAGGAGGTTAACTTATGGAAGCTCATGGGAGAGTTTAATCTTCATGGAATTACTGCCCCAGAGGAATATGGAGGGCTGGGTCTTGGCTACTTGTATCACTGCATTGCTGTGGAAGAAATAAGCCGTGCTTCAGGATCTGTTGGACTATCATATGGTGCCCATTCCAACCTGTGCATTAATCAATTGGTGAGGCATGGAAATCCTACTCAGAAAGAGAAGTATTTGCCAAAGCTTATTAGTGGAGAGCATGTAGGGGCTCTTGCAATGAGTGAGCCCAATTCTGGTTCTGATGTAGTTAGCATGAAGTGCAAAGCTGATCGAGCAGATGGGGGTTATATCTTAAATGGGAACAAGATGTGGTGCACCAATGGACCAGTTGCTCAAACATTGGTTGTTTATGCAAAAACGGACATAAATGCGGGCTCAAAGGGAATCACAGCGTTCATCATTGAGAAGGGGATGCCCGGATTCAGTACTGCCCAGAAATTAGACAAACTCGGCATGCGAGGAAGCGATACATGTGAGCTTGTCTTTGAGAATTGCTTTGTTCCGAATGACAATGTTCTTGGCCAGGAAGGAAAAGGAGTTTATGTCCTGATGTCAGGGTTAGATTTAGAGAGGCTTGTTTTAGCAGGTGGGCCCCTAGGTATTATGCAGGCATGTATGGATGTTGTCCTTCCTTATGTTCGACAGCGAGAGCAGTTTGGCCGTCCTATTGGAGAGTTTCAGCTTATACAGGGGAAAATTGCTGACATGTATACTTCTTTACAATCTTCAAGGGCGTATGTGTATTCTGTTGCAAGGGAATGTGACAATGGAAGAGTTGACCCAAAGGACTGTGCAGGAGTTATACTATCTACGGCTGAAAGAGCCACGCAAGTTGCTTTGCAGGCTATACAATGCTTAGGTGGTAATGGCTATGTAAATGAGTACCCAACTGGCCGTCTCCTTCGAGATGCCAAACTCTATGAGATTGGAGCAGGGACTAGTGAGATCAGAAGGATGATAATTGGTCGTGGGCTATTTAAGGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0003853 GO:0003995 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006139 GO:0006163 GO:0006195 GO:0006520 GO:0006551 GO:0006552 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008470 GO:0009056 GO:0009058 GO:0009063 GO:0009081 GO:0009083 GO:0009109 GO:0009117 GO:0009150 GO:0009154 GO:0009166 GO:0009259 GO:0009261 GO:0009987 GO:0016054 GO:0016491 GO:0016627 GO:0017076 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033869 GO:0033875 GO:0034031 GO:0034032 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035337 GO:0035383 GO:0035639 GO:0036094 GO:0036115 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046434 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0072521 GO:0072523 GO:0097159 GO:0097367 GO:1901135 GO:1901136 GO:1901265 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901568 GO:1901569 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1902189 GO:1902190 GO:1902192 GO:1902195 GO:1902196 GO:1902198
KEGG Pathways
Metabolic & Signaling
ko00280 ko01100 map00280 map01100
Pfam Domains
Protein Families
Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

409

Amino Acids

44.42

Weight (kDa)

7.06

Isoelectric Point (pI)

28.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-CoA_dh_N PF02771 32 - 145 1.5e-38 Acyl-CoA dehydrogenase, N-terminal domain
Acyl-CoA_dh_M PF02770 149 - 244 1.3e-25 Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1 PF00441 256 - 404 2.1e-44 Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2 PF08028 273 - 387 1.2e-10 Acyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 812
AccB1I GGYRCC 1 cut(s) 344
AccI GTMKAC 2 cut(s) 168, 947
AccII CGCG 1 cut(s) 62
AciI CCGC 2 cut(s) 62, 608
AclWI GGATC 1 cut(s) 331
AcoI YGGCCR 3 cut(s) 757, 895, 1129
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 2 cut(s) 664, 1121
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 2 cut(s) 707, 942
AgsI TTSAA 2 cut(s) 162, 966
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 1 cut(s) 759
AluBI AGCT 5 cut(s) 211, 421, 503, 715, 919
AluI AGCT 5 cut(s) 211, 421, 503, 715, 919
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 49, 1139
Alw44I GTGCAC 1 cut(s) 550
AlwI GGATC 1 cut(s) 331
AlwNI CAGNNNCTG 1 cut(s) 326
AoxI GGCC 4 cut(s) 757, 827, 895, 1129
ApaI GGGCCC 1 cut(s) 831
ApaLI GTGCAC 1 cut(s) 550
ApoI RAATTY 1 cut(s) 102
AseI ATTAAT 1 cut(s) 366
AspA2I CCTAGG 1 cut(s) 832
AspS9I GGNCC 3 cut(s) 560, 827, 828
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 388
AvaII GGWCC 1 cut(s) 560
AvrII CCTAGG 1 cut(s) 832
BaeGI GKGCMC 3 cut(s) 349, 554, 831
BalI TGGCCA 1 cut(s) 759
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 4 cut(s) 448, 465, 614, 831
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 554
BccI CCATC 1 cut(s) 509
BceAI ACGGC 4 cut(s) 297, 882, 1065, 1116
BciT130I CCWGG 1 cut(s) 761
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 2 cut(s) 49, 1139
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 2 cut(s) 833, 1176
BfuAI ACCTGC 1 cut(s) 812
BisI GCNGC 1 cut(s) 63
BlnI CCTAGG 1 cut(s) 832
BlsI GCNGC 1 cut(s) 64
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 2 cut(s) 654, 761
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 3 cut(s) 560, 827, 828
BmiI GGNNCC 3 cut(s) 346, 829, 830
BmrFI CCNGG 2 cut(s) 654, 761
BmsI GCATC 2 cut(s) 639, 1135
BpiI GAAGAC 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 1093
BpuMI CCSGG 1 cut(s) 654
BsaJI CCNNGG 2 cut(s) 189, 832
BsaXI ACNNNNNCTCC 4 cut(s) 423, 453, 765, 795
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 3 cut(s) 15, 563, 1132
Bse3DI GCAATG 3 cut(s) 144, 291, 459
BseBI CCWGG 1 cut(s) 761
BseDI CCNNGG 2 cut(s) 189, 832
BseGI GGATG 3 cut(s) 654, 862, 1197
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 3 cut(s) 144, 291, 459
BseMII CTCAG 2 cut(s) 58, 410
BseNI ACTGG 3 cut(s) 15, 563, 1132
BseSI GKGCMC 3 cut(s) 349, 554, 831
BseYI CCCAGC 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 1050
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 4 cut(s) 759, 829, 897, 1131
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 1 cut(s) 1186
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 49, 1139
BsmBI CGTCTC 1 cut(s) 1139
BsmFI GGGAC 1 cut(s) 1186
BsnI GGCC 4 cut(s) 759, 829, 897, 1131
Bsp120I GGGCCC 1 cut(s) 827
Bsp1286I GDGCHC 6 cut(s) 349, 448, 465, 554, 614, 831
Bsp143I GATC 3 cut(s) 323, 505, 1182
BspACI CCGC 2 cut(s) 62, 608
BspANI GGCC 4 cut(s) 759, 829, 897, 1131
BspCNI CTCAG 2 cut(s) 57, 409
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 346, 829, 830
BspMI ACCTGC 1 cut(s) 812
BspPI GGATC 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 344
BsrDI GCAATG 3 cut(s) 144, 291, 459
BsrI ACTGG 3 cut(s) 15, 563, 1132
BssECI CCNNGG 2 cut(s) 189, 832
BssMI GATC 3 cut(s) 323, 505, 1182
BssNAI GTATAC 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 189, 832
Bst1107I GTATAC 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 1 cut(s) 1028
Bst6I CTCTTC 2 cut(s) 92, 1003
BstAPI GCANNNNNTGC 1 cut(s) 495
BstC8I GCNNGC 4 cut(s) 610, 692, 846, 1080
BstDEI CTNAG 3 cut(s) 44, 396, 1093
BstF5I GGATG 3 cut(s) 654, 862, 1197
BstFNI CGCG 1 cut(s) 62
BstKTI GATC 3 cut(s) 326, 508, 1185
BstMAI GTCTC 2 cut(s) 49, 1139
BstMBI GATC 3 cut(s) 323, 505, 1182
BstMWI GCNNNNNNNGC 5 cut(s) 155, 495, 509, 1058, 1079
BstNI CCWGG 1 cut(s) 761
BstNSI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
BstSCI CCNGG 2 cut(s) 652, 759
BstSLI GKGCMC 3 cut(s) 349, 554, 831
BstUI CGCG 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BstZ17I GTATAC 1 cut(s) 948
BsuRI GGCC 4 cut(s) 759, 829, 897, 1131
BtsCI GGATG 3 cut(s) 654, 862, 1197
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 286
BveI ACCTGC 1 cut(s) 812
Cac8I GCNNGC 4 cut(s) 610, 692, 846, 1080
CaiI CAGNNNCTG 1 cut(s) 326
Cfr13I GGNCC 3 cut(s) 560, 827, 828
Csp6I GTAC 2 cut(s) 663, 1120
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 2 cut(s) 663, 1120
DdeI CTNAG 3 cut(s) 44, 396, 1093
DpnI GATC 3 cut(s) 325, 507, 1184
DpnII GATC 3 cut(s) 323, 505, 1182
EaeI YGGCCR 3 cut(s) 757, 895, 1129
Eam1104I CTCTTC 2 cut(s) 92, 1003
EarI CTCTTC 2 cut(s) 92, 1003
Eco130I CCWWGG 2 cut(s) 189, 832
Eco24I GRGCYC 4 cut(s) 448, 465, 614, 831
Eco47I GGWCC 1 cut(s) 560
Eco57I CTGAAG 1 cut(s) 303
EcoO109I RGGNCCY 1 cut(s) 828
EcoRII CCWGG 1 cut(s) 759
EcoT14I CCWWGG 2 cut(s) 189, 832
EcoT38I GRGCYC 4 cut(s) 448, 465, 614, 831
ErhI CCWWGG 2 cut(s) 189, 832
Esp3I CGTCTC 1 cut(s) 1139
FalI AAGNNNNNCTT 2 cut(s) 301, 333
FaqI GGGAC 1 cut(s) 1186
FauI CCCGC 1 cut(s) 601
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 2 cut(s) 168, 947
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 3 cut(s) 661, 869, 1204
FriOI GRGCYC 4 cut(s) 448, 465, 614, 831
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 2 cut(s) 833, 1176
GluI GCNGC 1 cut(s) 63
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 4 cut(s) 759, 829, 897, 1131
HapII CCGG 1 cut(s) 654
HincII GTYRAC 2 cut(s) 199, 1015
HindII GTYRAC 2 cut(s) 199, 1015
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 2 cut(s) 621, 657
HpaI GTTAAC 1 cut(s) 199
HpaII CCGG 1 cut(s) 654
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 5 cut(s) 169, 199, 552, 948, 1015
Hpy188I TCNGA 6 cut(s) 47, 54, 399, 478, 741, 1187
Hpy188III TCNNGA 3 cut(s) 321, 784, 1142
Hpy8I GTNNAC 5 cut(s) 169, 199, 552, 948, 1015
HpyAV CCTTC 5 cut(s) 637, 758, 875, 1148, 1182
HpyCH4III ACNGT 1 cut(s) 1028
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 495, 509, 1058, 1079
HpyF3I CTNAG 3 cut(s) 44, 396, 1093
KspAI GTTAAC 1 cut(s) 199
Kzo9I GATC 3 cut(s) 323, 505, 1182
LmnI GCTCC 2 cut(s) 1166, 1219
LweI GCATC 2 cut(s) 639, 1135
MaeI CTAG 2 cut(s) 833, 1176
MaeIII GTNAC 1 cut(s) 998
MalI GATC 3 cut(s) 325, 507, 1184
MboI GATC 3 cut(s) 323, 505, 1182
MboII GAAGA 6 cut(s) 32, 79, 221, 314, 954, 1020
MfeI CAATTG 1 cut(s) 371
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 6 cut(s) 349, 448, 465, 554, 614, 831
MlsI TGGCCA 1 cut(s) 759
MluNI TGGCCA 1 cut(s) 759
MmeI TCCRAC 2 cut(s) 310, 378
MnlI CCTC 8 cut(s) 15, 88, 187, 244, 254, 372, 689, 801
Mox20I TGGCCA 1 cut(s) 759
MscI TGGCCA 1 cut(s) 759
MseI TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
MslI CAYNNNNRTG 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 759
MspI CCGG 1 cut(s) 654
MspR9I CCNGG 2 cut(s) 654, 761
MunI CAATTG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 761
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 5 cut(s) 155, 495, 509, 1058, 1079
NciI CCSGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 340
NdeII GATC 3 cut(s) 323, 505, 1182
NlaIV GGNNCC 3 cut(s) 346, 829, 830
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 998
NspI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
PaeI GCATGC 1 cut(s) 694
PaqCI CACCTGC 1 cut(s) 812
PciI ACATGT 2 cut(s) 707, 942
PfeI GAWTC 2 cut(s) 621, 657
PkrI GCNGC 1 cut(s) 64
PscI ACATGT 2 cut(s) 707, 942
PshBI ATTAAT 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 759
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 3 cut(s) 346, 829, 830
PspOMI GGGCCC 1 cut(s) 827
PspPI GGNCC 3 cut(s) 560, 827, 828
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 2 cut(s) 664, 1121
RsaNI GTAC 2 cut(s) 663, 1120
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 3 cut(s) 323, 505, 1182
Sau96I GGNCC 3 cut(s) 560, 827, 828
ScaI AGTACT 1 cut(s) 664
ScrFI CCNGG 2 cut(s) 654, 761
SduI GDGCHC 6 cut(s) 349, 448, 465, 554, 614, 831
SfaNI GCATC 2 cut(s) 639, 1135
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 296
SpeI ACTAGT 1 cut(s) 1175
SphI GCATGC 1 cut(s) 694
SsiI CCGC 2 cut(s) 62, 608
SspMI CTAG 2 cut(s) 833, 1176
StyD4I CCNGG 2 cut(s) 652, 759
StyI CCWWGG 2 cut(s) 189, 832
TaaI ACNGT 1 cut(s) 1028
TaqI TCGA 4 cut(s) 90, 508, 877, 1141
TatI WGTACW 1 cut(s) 662
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 621, 657
Tru1I TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
Tru9I TTAA 6 cut(s) 111, 198, 225, 366, 530, 1215
TscAI CASTG 1 cut(s) 293
TseFI GTSAC 1 cut(s) 998
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 3 cut(s) 221, 506, 622
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 1 cut(s) 293
VneI GTGCAC 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 560
VspI ATTAAT 1 cut(s) 366
XapI RAATTY 1 cut(s) 102
XceI RCATGY 6 cut(s) 158, 440, 694, 711, 852, 946
XmaJI CCTAGG 1 cut(s) 832
XmiI GTMKAC 2 cut(s) 168, 947
XspI CTAG 2 cut(s) 833, 1176
ZrmI AGTACT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.