MD03G1116100.v1.1

Isovaleryl-CoA dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006675
Physical Location & Seq
Reverse (-)
10316249 .. 10322464
6216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1116100.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGCAAAGGCTATTTGCTGCGAGGTCTACATGCGCTGCGATTGTCAGAAATCAGAGAAAGTTTGCTTCTTTTTCTACCGCCTTACTCTTCGATGATACTCAGATTCAGTTTAAAGAAAGTGTTGCTCAGTTTGCACAAGAAAACATCGCCCCTCATGCGTCGAAAATAGACCACACAAATTATTTACCTCAGGAGGTCAACTTATGGAAACTCATGGGGGATTTTAATCTTCATGGAATTACTGCACCAGAGGAATACGGAGGTCTTGGTCTTGGTTACTTGTATCACTGTATAGCTATGGAAGAAATAAGCCGTGCTTCAGGGTCTGTTGGCCTCTCATACGGTGCCCATTCTAACCTGTGCATCAATCAATTGGTGAGGCATGGAAACCCTACTCAGAAACAGAAGTATTTGCCAAAGCTTATCAGTGGAGAGCATATAGGGGCTCTTGCAATGAGTGAACCCAATGCTGGTTCTGACGTTGTTAGCATGAAATGCAAGGCTGATCGAGTAGATGGGGGTTATGTCTTAAATGGGAACAAGATGTGGTGCACCAATGGCCCAGTTGCTCAAACATTGGTTATTTATGCAAAAACAGACATCGCAGCGGGCTCAAAAGGAATCACAGCATTCATCATTGAGAAAGGAACACCCGGATTCAGTACCGCCCAGAAATTAGACAAACTTGGCATGCGAGGAAGTGATACATGTGAACTTGTCTTTGAGAACTGCTTTGTTCCAGAAGAAAATGTTCTCGGGCAGGAAGGAAAAGGTGTCTATGTCATGATGTCAGGGCTGGATCTAGAGAGGCTTGTTTTAGCAGGTGGGCCCCTGGGTATTATGCAGGCATGTATGGATGCTGTCCTTCCTTATGTTCAACAGCGAGAGCAGTTTGGCCGACCCATTGGAGAATTTCAGCTTATACAGGGGAAACTTGCTGACATGTATACCTCTTTGCAATCATCAAGGTCCTACGTGTATTCTGTTGCAAGGGAGTGTGACAATGGAAAAATTGACCCTAAGGATTGTGCTGGAGTTATACTATGTACGGCTGAAAGAGCCACGCAGGTTGCTTTGCAGGCTATACAATGCTTAGGTGGTAACGGTTATGTCAATGAGTACCCAACTGGGCGGCTCCTTCGAGATGCCAAACTGTATGAGATTGGAGCTGGGACTAGTGAGATCAGAAGGATGATAATAGGCCGTGCGCTATTTAAGGATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0003853 GO:0003995 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006139 GO:0006163 GO:0006195 GO:0006520 GO:0006551 GO:0006552 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008470 GO:0009056 GO:0009058 GO:0009063 GO:0009081 GO:0009083 GO:0009109 GO:0009117 GO:0009150 GO:0009154 GO:0009166 GO:0009259 GO:0009261 GO:0009987 GO:0016054 GO:0016491 GO:0016627 GO:0017076 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033869 GO:0033875 GO:0034031 GO:0034032 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035337 GO:0035383 GO:0035639 GO:0036094 GO:0036115 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046434 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0072521 GO:0072523 GO:0097159 GO:0097367 GO:1901135 GO:1901136 GO:1901265 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901568 GO:1901569 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1902189 GO:1902190 GO:1902192 GO:1902195 GO:1902196 GO:1902198
KEGG Pathways
Metabolic & Signaling
ko00280 ko01100 map00280 map01100
Pfam Domains
Protein Families
Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

409

Amino Acids

44.48

Weight (kDa)

6.66

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-CoA_dh_N PF02771 32 - 145 5.1e-38 Acyl-CoA dehydrogenase, N-terminal domain
Acyl-CoA_dh_M PF02770 149 - 244 3.7e-25 Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1 PF00441 256 - 404 2.1e-45 Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2 PF08028 273 - 387 1.7e-10 Acyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 812
Acc36I ACCTGC 2 cut(s) 812, 1057
AccB1I GGYRCC 1 cut(s) 344
AccI GTMKAC 2 cut(s) 26, 947
AciI CCGC 4 cut(s) 78, 608, 666, 1132
AclWI GGATC 1 cut(s) 807
AcoI YGGCCR 1 cut(s) 895
AcsI RAATTY 1 cut(s) 911
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 3 cut(s) 664, 1048, 1121
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 3 cut(s) 707, 942, 975
AgsI TTSAA 1 cut(s) 878
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 1 cut(s) 831
AluBI AGCT 4 cut(s) 296, 421, 919, 1169
AluI AGCT 4 cut(s) 296, 421, 919, 1169
Alw21I GWGCWC 1 cut(s) 554
Alw44I GTGCAC 1 cut(s) 550
AlwI GGATC 1 cut(s) 807
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 1 cut(s) 755
AoxI GGCC 5 cut(s) 331, 559, 827, 895, 1201
ApaI GGGCCC 1 cut(s) 831
ApaLI GTGCAC 1 cut(s) 550
ApeKI GCWGC 3 cut(s) 17, 35, 605
ApoI RAATTY 1 cut(s) 911
Asp700I GAANNNNTTC 1 cut(s) 750
AspLEI GCGC 2 cut(s) 35, 1210
AspS9I GGNCC 4 cut(s) 560, 827, 828, 969
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 755
AvaII GGWCC 1 cut(s) 969
AxyI CCTNAGG 2 cut(s) 189, 1020
BaeGI GKGCMC 3 cut(s) 349, 554, 831
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 3 cut(s) 448, 614, 831
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 3 cut(s) 4, 22, 617
BccI CCATC 1 cut(s) 509
BceAI ACGGC 3 cut(s) 297, 1065, 1188
BciT130I CCWGG 1 cut(s) 833
BcnI CCSGG 1 cut(s) 654
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 2 cut(s) 803, 1176
BfuAI ACCTGC 2 cut(s) 812, 1057
BisI GCNGC 4 cut(s) 18, 36, 606, 1133
BlsI GCNGC 4 cut(s) 19, 37, 607, 1134
Bme1390I CCNGG 2 cut(s) 654, 833
Bme18I GGWCC 1 cut(s) 969
BmeT110I CYCGRG 1 cut(s) 755
BmgT120I GGNCC 4 cut(s) 560, 827, 828, 969
BmiI GGNNCC 4 cut(s) 346, 829, 830, 1136
BmrFI CCNGG 2 cut(s) 654, 833
BmrI ACTGGG 2 cut(s) 557, 1137
BmsI GCATC 3 cut(s) 372, 847, 1135
BmuI ACTGGG 2 cut(s) 557, 1137
BpmI CTGGAG 1 cut(s) 1053
Bpu10I CCTNAGC 1 cut(s) 1093
BpuMI CCSGG 1 cut(s) 654
BsaAI YACGTR 1 cut(s) 976
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 831, 832
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 563, 1132
Bse21I CCTNAGG 2 cut(s) 189, 1020
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 1 cut(s) 833
BseDI CCNNGG 2 cut(s) 831, 832
BseGI GGATG 2 cut(s) 862, 1197
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 4 cut(s) 113, 140, 203, 410
BseNI ACTGG 2 cut(s) 563, 1132
BseSI GKGCMC 3 cut(s) 349, 554, 831
BseXI GCAGC 3 cut(s) 4, 22, 617
BseYI CCCAGC 1 cut(s) 1169
BsgI GTGCAG 1 cut(s) 228
BshFI GGCC 5 cut(s) 333, 561, 829, 897, 1203
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 755
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 1 cut(s) 1186
BslI CCNNNNNNNGG 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 1186
BsmI GAATGC 1 cut(s) 629
BsnI GGCC 5 cut(s) 333, 561, 829, 897, 1203
BsoBI CYCGRG 1 cut(s) 755
Bsp120I GGGCCC 1 cut(s) 827
Bsp1286I GDGCHC 5 cut(s) 349, 448, 554, 614, 831
Bsp143I GATC 4 cut(s) 505, 799, 1182, 1219
BspACI CCGC 4 cut(s) 78, 608, 666, 1132
BspANI GGCC 5 cut(s) 333, 561, 829, 897, 1203
BspCNI CTCAG 4 cut(s) 112, 139, 202, 409
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 4 cut(s) 346, 829, 830, 1136
BspMI ACCTGC 2 cut(s) 812, 1057
BspPI GGATC 1 cut(s) 807
BspT107I GGYRCC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 2 cut(s) 563, 1132
BssECI CCNNGG 2 cut(s) 831, 832
BssMI GATC 4 cut(s) 505, 799, 1182, 1219
BssNAI GTATAC 1 cut(s) 948
Bst1107I GTATAC 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 4 cut(s) 290, 344, 1106, 1155
Bst6I CTCTTC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 495
BstBAI YACGTR 1 cut(s) 976
BstC8I GCNNGC 4 cut(s) 610, 692, 846, 1080
BstDEI CTNAG 6 cut(s) 99, 126, 189, 396, 1020, 1093
BstF5I GGATG 2 cut(s) 862, 1197
BstHHI GCGC 2 cut(s) 35, 1210
BstKTI GATC 4 cut(s) 508, 802, 1185, 1222
BstMBI GATC 4 cut(s) 505, 799, 1182, 1219
BstMWI GCNNNNNNNGC 6 cut(s) 131, 155, 495, 558, 1058, 1079
BstNI CCWGG 1 cut(s) 833
BstNSI RCATGY 5 cut(s) 33, 694, 711, 852, 946
BstSCI CCNGG 2 cut(s) 652, 831
BstSLI GKGCMC 3 cut(s) 349, 554, 831
BstV1I GCAGC 3 cut(s) 4, 22, 617
BstX2I RGATCY 1 cut(s) 799
BstYI RGATCY 1 cut(s) 799
BstZ17I GTATAC 1 cut(s) 948
Bsu36I CCTNAGG 2 cut(s) 189, 1020
BsuRI GGCC 5 cut(s) 333, 561, 829, 897, 1203
BtgZI GCGATG 2 cut(s) 130, 586
BtsCI GGATG 2 cut(s) 862, 1197
BtsIMutI CAGTG 2 cut(s) 286, 433
BveI ACCTGC 2 cut(s) 812, 1057
Cac8I GCNNGC 4 cut(s) 610, 692, 846, 1080
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 1 cut(s) 783
CfoI GCGC 2 cut(s) 35, 1210
Cfr13I GGNCC 4 cut(s) 560, 827, 828, 969
CseI GACGC 1 cut(s) 147
Csp6I GTAC 3 cut(s) 663, 1047, 1120
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 3 cut(s) 663, 1047, 1120
DdeI CTNAG 6 cut(s) 99, 126, 189, 396, 1020, 1093
DpnI GATC 4 cut(s) 507, 801, 1184, 1221
DpnII GATC 4 cut(s) 505, 799, 1182, 1219
DraI TTTAAA 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 895
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco24I GRGCYC 3 cut(s) 448, 614, 831
Eco47I GGWCC 1 cut(s) 969
Eco57I CTGAAG 1 cut(s) 303
Eco81I CCTNAGG 2 cut(s) 189, 1020
Eco88I CYCGRG 1 cut(s) 755
EcoO109I RGGNCCY 2 cut(s) 828, 969
EcoRII CCWGG 1 cut(s) 831
EcoT38I GRGCYC 3 cut(s) 448, 614, 831
FalI AAGNNNNNCTT 2 cut(s) 301, 333
FaqI GGGAC 1 cut(s) 1186
FauI CCCGC 1 cut(s) 601
FblI GTMKAC 2 cut(s) 26, 947
Fnu4HI GCNGC 4 cut(s) 18, 36, 606, 1133
FokI GGATG 2 cut(s) 869, 1204
FriOI GRGCYC 3 cut(s) 448, 614, 831
Fsp4HI GCNGC 4 cut(s) 18, 36, 606, 1133
FspBI CTAG 2 cut(s) 803, 1176
GlaI GCGC 2 cut(s) 34, 1209
GluI GCNGC 4 cut(s) 18, 36, 606, 1133
GsaI CCCAGC 1 cut(s) 1173
GsuI CTGGAG 1 cut(s) 1053
HaeIII GGCC 5 cut(s) 333, 561, 829, 897, 1203
HapII CCGG 1 cut(s) 654
HgaI GACGC 1 cut(s) 147
HhaI GCGC 2 cut(s) 35, 1210
Hin6I GCGC 2 cut(s) 33, 1208
HinP1I GCGC 2 cut(s) 33, 1208
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 3 cut(s) 103, 621, 657
HpaII CCGG 1 cut(s) 654
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 6 cut(s) 27, 199, 461, 552, 713, 948
Hpy188I TCNGA 6 cut(s) 47, 54, 102, 399, 478, 1187
Hpy188III TCNNGA 5 cut(s) 191, 740, 784, 803, 1142
Hpy8I GTNNAC 6 cut(s) 27, 199, 461, 552, 713, 948
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 4 cut(s) 758, 875, 1148, 1182
HpyCH4III ACNGT 4 cut(s) 290, 344, 1106, 1155
HpyCH4IV ACGT 2 cut(s) 480, 975
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 155, 495, 558, 1058, 1079
HpyF3I CTNAG 6 cut(s) 99, 126, 189, 396, 1020, 1093
HpySE526I ACGT 2 cut(s) 480, 975
HspAI GCGC 2 cut(s) 33, 1208
Kzo9I GATC 4 cut(s) 505, 799, 1182, 1219
LmnI GCTCC 2 cut(s) 1140, 1166
Lsp1109I GCAGC 3 cut(s) 4, 22, 617
LweI GCATC 3 cut(s) 372, 847, 1135
MaeI CTAG 2 cut(s) 803, 1176
MaeII ACGT 2 cut(s) 480, 975
MaeIII GTNAC 3 cut(s) 275, 998, 1100
MalI GATC 4 cut(s) 507, 801, 1184, 1221
MboI GATC 4 cut(s) 505, 799, 1182, 1219
MboII GAAGA 4 cut(s) 79, 221, 314, 755
MfeI CAATTG 1 cut(s) 371
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 5 cut(s) 349, 448, 554, 614, 831
MluCI AATT 6 cut(s) 178, 237, 371, 674, 911, 1011
MroXI GAANNNNTTC 1 cut(s) 750
MseI TTAA 4 cut(s) 111, 225, 530, 1215
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 1 cut(s) 654
MspR9I CCNGG 2 cut(s) 654, 833
MunI CAATTG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 629
MvaI CCWGG 1 cut(s) 833
MwoI GCNNNNNNNGC 6 cut(s) 131, 155, 495, 558, 1058, 1079
NciI CCSGG 1 cut(s) 654
NdeII GATC 4 cut(s) 505, 799, 1182, 1219
NlaIV GGNNCC 4 cut(s) 346, 829, 830, 1136
NmuCI GTSAC 1 cut(s) 998
NspI RCATGY 5 cut(s) 33, 694, 711, 852, 946
PaeI GCATGC 1 cut(s) 694
PagI TCATGA 1 cut(s) 783
PaqCI CACCTGC 1 cut(s) 812
PasI CCCWGGG 1 cut(s) 832
PciI ACATGT 2 cut(s) 707, 942
PctI GAATGC 1 cut(s) 629
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 3 cut(s) 103, 621, 657
PkrI GCNGC 4 cut(s) 19, 37, 607, 1134
Ppu21I YACGTR 1 cut(s) 976
PpuMI RGGWCCY 1 cut(s) 969
PscI ACATGT 2 cut(s) 707, 942
Psp5II RGGWCCY 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 831
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 1 cut(s) 831
PspN4I GGNNCC 4 cut(s) 346, 829, 830, 1136
PspOMI GGGCCC 1 cut(s) 827
PspPI GGNCC 4 cut(s) 560, 827, 828, 969
PspPPI RGGWCCY 1 cut(s) 969
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 1 cut(s) 799
RsaI GTAC 3 cut(s) 664, 1048, 1121
RsaNI GTAC 3 cut(s) 663, 1047, 1120
SaqAI TTAA 4 cut(s) 111, 225, 530, 1215
SatI GCNGC 4 cut(s) 18, 36, 606, 1133
Sau3AI GATC 4 cut(s) 505, 799, 1182, 1219
Sau96I GGNCC 4 cut(s) 560, 827, 828, 969
ScrFI CCNGG 2 cut(s) 654, 833
SduI GDGCHC 5 cut(s) 349, 448, 554, 614, 831
SfaNI GCATC 3 cut(s) 372, 847, 1135
SinI GGWCC 1 cut(s) 969
SpeI ACTAGT 1 cut(s) 1175
SphI GCATGC 1 cut(s) 694
Sse9I AATT 6 cut(s) 178, 237, 371, 674, 911, 1011
SsiI CCGC 4 cut(s) 78, 608, 666, 1132
SspMI CTAG 2 cut(s) 803, 1176
StyD4I CCNGG 2 cut(s) 652, 831
TaaI ACNGT 4 cut(s) 290, 344, 1106, 1155
TaiI ACGT 2 cut(s) 483, 978
TaqI TCGA 4 cut(s) 90, 161, 508, 1141
TasI AATT 6 cut(s) 178, 237, 371, 674, 911, 1011
TauI GCSGC 1 cut(s) 1135
TfiI GAWTC 3 cut(s) 103, 621, 657
Tru1I TTAA 4 cut(s) 111, 225, 530, 1215
Tru9I TTAA 4 cut(s) 111, 225, 530, 1215
TscAI CASTG 2 cut(s) 293, 433
TseFI GTSAC 1 cut(s) 998
TseI GCWGC 3 cut(s) 17, 35, 605
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 3 cut(s) 221, 506, 622
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 2 cut(s) 293, 433
VneI GTGCAC 1 cut(s) 550
VpaK11BI GGWCC 1 cut(s) 969
XapI RAATTY 1 cut(s) 911
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 5 cut(s) 33, 694, 711, 852, 946
XmiI GTMKAC 2 cut(s) 26, 947
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 2 cut(s) 803, 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.