Rorug05G0327800

Isovaleryl-CoA dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006675
Physical Location & Seq
Forward (+)
39436403 .. 39437584
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0327800.1

Sequence Viewer

Length: 1182 bp
ATGGAGGGTGGTCGTATGCTTCTTCCAGGCCAAAGGTTTTGCCCCATGGAAGATGAACTACTCATGCTCTACCTTAAGCCTAAGGTGAACGGGATGGAAGTGCCCGGCAACGAAGACGTTATCTGTGAGTTGGATCTTTACGGTGACAACGATCCTTGGAAGATATGGGAAAGATTCGAAGCAAGAAAGGCCAACGACTTGAGGAGGAACAAAGATCTCTACTTCTTCACCCAAAAGAAGAAAAAGACTGCAAAAAGCTCACGTATAAGCCGAACTGTTGGGAGTGGCGGTACTTGGAAAGGCCAGAATGCAGCCAAGGAGATCTATCTTCTTGATGATAATCAGCAGCCAACGACTACTCTTCTTGGTTTCAAGAAAACCTACACCTATAAGAACAAGGGGTCTGTGCATGATGGTCGCTGGATCATGTATGAGTTTGAACTTGATAAGTCACAACTCGTGCACAAGAAACAAGTAAACAAGAATGACTATGTTCTTTGTTTACTTAGGAAAAACGATGTACTGCCGGAAAGAAAGAGAAAAAGGCAAGAAGAAGAGGAGATGCTTGGAGACTATGTCGAGGACCATGACGGAGATAACACAGATCCTGAATCGGTTACTGAGGAGCCGCAAGAGAAACGGCAACGTCTTCTACCATCATTAGAAGCTGAACAAGAACAATTCTTGCAGCCAGCACCATCATTAGAATCTGAACAAGAAGCAGTACAAGATGAACCACTTCTCTTGCAAGTGGATGAGAATATGGAACTGCAGCAACTGGAGTTGGCTGAACTAGAAAAGTGGTTTGAAGTGGAATTTGAAGCAAGACCTACAACACTAGAGGCTGAACAAGTTTCCTTGCCTGTTGATGAGAATTCAGGACTGCAACAATTGGAAGCTGAACCTCAACTAGGAGGAGAAAATCTGGAGCAGCAACTTGATGCACCATCATTTGCAGCTGATGAGATAATGAGTGGCTTTCAGTTTCACCCAGATGAAAATCCGGCGAAGCAAATGGGGGCTGAACCCAATGTGTATGGAGGGAACTTGAGTGACGCTATGGATGGTGAAAACTGGCCTATGTTTTTTGTGGAAGAGCTAATGAATGATGAGCAGCCAAATACCATGGAAATGGGAGAGTGGAATGCTACTTTTTCTGATTTTGAGTACTTAGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0003853 GO:0003995 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006139 GO:0006163 GO:0006195 GO:0006520 GO:0006551 GO:0006552 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008470 GO:0009056 GO:0009058 GO:0009063 GO:0009081 GO:0009083 GO:0009109 GO:0009117 GO:0009150 GO:0009154 GO:0009166 GO:0009259 GO:0009261 GO:0009987 GO:0016054 GO:0016491 GO:0016627 GO:0017076 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033869 GO:0033875 GO:0034031 GO:0034032 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035337 GO:0035383 GO:0035639 GO:0036094 GO:0036115 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046434 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0072521 GO:0072523 GO:0097159 GO:0097367 GO:1901135 GO:1901136 GO:1901265 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901568 GO:1901569 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1902189 GO:1902190 GO:1902192 GO:1902195 GO:1902196 GO:1902198
KEGG Pathways
Metabolic & Signaling
ko00280 ko01100 map00280 map01100
Pfam Domains
Protein Families
Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

393

Amino Acids

45.48

Weight (kDa)

4.44

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 148 8.9e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 288, 629
AclWI GGATC 4 cut(s) 141, 146, 431, 599
AcsI RAATTY 2 cut(s) 815, 874
AfaI GTAC 4 cut(s) 292, 522, 726, 1169
AfiI CCNNNNNNNGG 1 cut(s) 911
AflII CTTAAG 1 cut(s) 74
AgsI TTSAA 4 cut(s) 373, 440, 809, 821
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 5 cut(s) 258, 668, 899, 959, 1099
AluI AGCT 5 cut(s) 258, 668, 899, 959, 1099
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 1 cut(s) 564
Alw44I GTGCAC 1 cut(s) 461
AlwI GGATC 4 cut(s) 141, 146, 431, 599
AlwNI CAGNNNCTG 2 cut(s) 608, 778
AoxI GGCC 4 cut(s) 28, 189, 301, 1076
ApaLI GTGCAC 1 cut(s) 461
ApeKI GCWGC 7 cut(s) 311, 346, 688, 772, 931, 956, 1114
ApoI RAATTY 2 cut(s) 815, 874
Asp700I GAANNNNTTC 1 cut(s) 738
AspS9I GGNCC 1 cut(s) 583
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 5 cut(s) 97, 155, 220, 980, 1079
AsuII TTCGAA 1 cut(s) 177
AvaII GGWCC 1 cut(s) 583
AxyI CCTNAGG 1 cut(s) 81
BaeGI GKGCMC 2 cut(s) 105, 465
BarI GAAGNNNNNNTAC 2 cut(s) 42, 74
BauI CACGAG 1 cut(s) 458
BbsI GAAGAC 2 cut(s) 120, 641
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 7 cut(s) 323, 358, 700, 784, 943, 968, 1126
BccI CCATC 6 cut(s) 88, 407, 664, 706, 955, 1058
BceAI ACGGC 1 cut(s) 656
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 1 cut(s) 27
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 3 cut(s) 794, 839, 911
BfmI CTRYAG 1 cut(s) 770
BfrI CTTAAG 1 cut(s) 74
BglII AGATCT 2 cut(s) 214, 321
BisI GCNGC 8 cut(s) 312, 347, 629, 689, 773, 932, 957, 1115
BlsI GCNGC 8 cut(s) 313, 348, 630, 690, 774, 933, 958, 1116
BmcAI AGTACT 1 cut(s) 1169
Bme1390I CCNGG 2 cut(s) 27, 105
Bme18I GGWCC 1 cut(s) 583
BmgT120I GGNCC 1 cut(s) 583
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 2 cut(s) 27, 105
BmsI GCATC 2 cut(s) 552, 931
BpiI GAAGAC 2 cut(s) 120, 641
BpmI CTGGAG 2 cut(s) 800, 947
Bpu14I TTCGAA 1 cut(s) 177
BpuEI CTTGAG 2 cut(s) 220, 1069
BpuMI CCSGG 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 263
BsaBI GATNNNNATC 2 cut(s) 339, 999
BsaJI CCNNGG 4 cut(s) 45, 155, 315, 1125
BsaXI ACNNNNNCTCC 4 cut(s) 274, 304, 561, 591
Bsc4I CCNNNNNNNGG 1 cut(s) 911
Bse1I ACTGG 2 cut(s) 783, 1079
Bse21I CCTNAGG 1 cut(s) 81
Bse8I GATNNNNATC 2 cut(s) 339, 999
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 4 cut(s) 45, 155, 315, 1125
BseGI GGATG 3 cut(s) 99, 760, 1069
BseJI GATNNNNATC 2 cut(s) 339, 999
BseLI CCNNNNNNNGG 1 cut(s) 911
BseMII CTCAG 1 cut(s) 612
BseNI ACTGG 2 cut(s) 783, 1079
BseRI GAGGAG 4 cut(s) 217, 572, 638, 930
BseSI GKGCMC 2 cut(s) 105, 465
BseXI GCAGC 7 cut(s) 323, 358, 700, 784, 943, 968, 1126
BshFI GGCC 4 cut(s) 30, 191, 303, 1078
BsiHKAI GWGCWC 1 cut(s) 465
BsiSI CCGG 3 cut(s) 105, 527, 1004
BslI CCNNNNNNNGG 1 cut(s) 911
BsmAI GTCTC 1 cut(s) 564
BsmI GAATGC 2 cut(s) 313, 1150
BsnI GGCC 4 cut(s) 30, 191, 303, 1078
Bsp119I TTCGAA 1 cut(s) 177
Bsp1286I GDGCHC 2 cut(s) 105, 465
Bsp143I GATC 6 cut(s) 133, 151, 214, 321, 423, 604
Bsp19I CCATGG 2 cut(s) 45, 1125
BspACI CCGC 2 cut(s) 288, 629
BspANI GGCC 4 cut(s) 30, 191, 303, 1078
BspCNI CTCAG 1 cut(s) 613
BspLI GGNNCC 1 cut(s) 627
BspMAI CTGCAG 1 cut(s) 774
BspPI GGATC 4 cut(s) 141, 146, 431, 599
BspQI GCTCTTC 1 cut(s) 1089
BspT104I TTCGAA 1 cut(s) 177
BspTI CTTAAG 1 cut(s) 74
BsrI ACTGG 2 cut(s) 783, 1079
BssECI CCNNGG 4 cut(s) 45, 155, 315, 1125
BssMI GATC 6 cut(s) 133, 151, 214, 321, 423, 604
BssSI CACGAG 1 cut(s) 458
BssT1I CCWWGG 4 cut(s) 45, 155, 315, 1125
Bst2BI CACGAG 1 cut(s) 458
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 143, 277
Bst6I CTCTTC 3 cut(s) 366, 549, 1089
BstAFI CTTAAG 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 693
BstDEI CTNAG 4 cut(s) 81, 506, 621, 1171
BstDSI CCRYGG 2 cut(s) 45, 1125
BstENI CCTNNNNNAGG 1 cut(s) 909
BstF5I GGATG 3 cut(s) 99, 760, 1069
BstKTI GATC 6 cut(s) 136, 154, 217, 324, 426, 607
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 6 cut(s) 133, 151, 214, 321, 423, 604
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNI CCWGG 1 cut(s) 27
BstSCI CCNGG 2 cut(s) 25, 103
BstSFI CTRYAG 1 cut(s) 770
BstSLI GKGCMC 2 cut(s) 105, 465
BstV1I GCAGC 7 cut(s) 323, 358, 700, 784, 943, 968, 1126
BstV2I GAAGAC 2 cut(s) 120, 641
BstX2I RGATCY 4 cut(s) 133, 214, 321, 604
BstXI CCANNNNNNTGG 1 cut(s) 1132
BstYI RGATCY 4 cut(s) 133, 214, 321, 604
Bsu36I CCTNAGG 1 cut(s) 81
BsuRI GGCC 4 cut(s) 30, 191, 303, 1078
BtgI CCRYGG 2 cut(s) 45, 1125
BtsCI GGATG 3 cut(s) 99, 760, 1069
Cac8I GCNNGC 1 cut(s) 693
CaiI CAGNNNCTG 2 cut(s) 608, 778
Cfr13I GGNCC 1 cut(s) 583
CseI GACGC 1 cut(s) 1064
Csp6I GTAC 4 cut(s) 291, 521, 725, 1168
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 6 cut(s) 46, 64, 410, 427, 587, 1126
CviQI GTAC 4 cut(s) 291, 521, 725, 1168
DdeI CTNAG 4 cut(s) 81, 506, 621, 1171
DpnI GATC 6 cut(s) 135, 153, 216, 323, 425, 606
DpnII GATC 6 cut(s) 133, 151, 214, 321, 423, 604
Eam1104I CTCTTC 3 cut(s) 366, 549, 1089
EarI CTCTTC 3 cut(s) 366, 549, 1089
Eco130I CCWWGG 4 cut(s) 45, 155, 315, 1125
Eco47I GGWCC 1 cut(s) 583
Eco81I CCTNAGG 1 cut(s) 81
EcoNI CCTNNNNNAGG 1 cut(s) 909
EcoRI GAATTC 1 cut(s) 874
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 4 cut(s) 45, 155, 315, 1125
ErhI CCWWGG 4 cut(s) 45, 155, 315, 1125
FaeI CATG 6 cut(s) 49, 67, 413, 430, 590, 1129
FatI CATG 6 cut(s) 45, 63, 409, 426, 586, 1125
Fnu4HI GCNGC 8 cut(s) 312, 347, 629, 689, 773, 932, 957, 1115
FokI GGATG 3 cut(s) 106, 767, 1076
Fsp4HI GCNGC 8 cut(s) 312, 347, 629, 689, 773, 932, 957, 1115
FspBI CTAG 3 cut(s) 794, 839, 911
GluI GCNGC 8 cut(s) 312, 347, 629, 689, 773, 932, 957, 1115
GsuI CTGGAG 2 cut(s) 800, 947
HaeIII GGCC 4 cut(s) 30, 191, 303, 1078
HapII CCGG 3 cut(s) 105, 527, 1004
HgaI GACGC 1 cut(s) 1064
Hin1II CATG 6 cut(s) 49, 67, 413, 430, 590, 1129
HinfI GANTC 3 cut(s) 174, 611, 707
HpaII CCGG 3 cut(s) 105, 527, 1004
HphI GGTGA 5 cut(s) 97, 155, 220, 980, 1079
Hpy166II GTNNAC 4 cut(s) 88, 463, 478, 503
Hpy188I TCNGA 2 cut(s) 712, 1159
Hpy188III TCNNGA 5 cut(s) 332, 373, 608, 879, 926
Hpy8I GTNNAC 4 cut(s) 88, 463, 478, 503
HpyCH4III ACNGT 2 cut(s) 143, 277
HpyCH4IV ACGT 3 cut(s) 117, 262, 646
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 4 cut(s) 81, 506, 621, 1171
HpySE526I ACGT 3 cut(s) 117, 262, 646
Hsp92II CATG 6 cut(s) 49, 67, 413, 430, 590, 1129
Kzo9I GATC 6 cut(s) 133, 151, 214, 321, 423, 604
LguI GCTCTTC 1 cut(s) 1089
LmnI GCTCC 2 cut(s) 625, 928
Lsp1109I GCAGC 7 cut(s) 323, 358, 700, 784, 943, 968, 1126
LweI GCATC 2 cut(s) 552, 931
MaeI CTAG 3 cut(s) 794, 839, 911
MaeII ACGT 3 cut(s) 117, 262, 646
MaeIII GTNAC 4 cut(s) 143, 450, 616, 1052
MalI GATC 6 cut(s) 135, 153, 216, 323, 425, 606
MboI GATC 6 cut(s) 133, 151, 214, 321, 423, 604
MfeI CAATTG 1 cut(s) 890
MflI RGATCY 4 cut(s) 133, 214, 321, 604
MhlI GDGCHC 2 cut(s) 105, 465
MluCI AATT 4 cut(s) 680, 815, 874, 890
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 9 cut(s) 195, 198, 550, 574, 616, 835, 908, 915, 1034
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 1 cut(s) 75
MslI CAYNNNNRTG 2 cut(s) 993, 1130
MspA1I CMGCKG 1 cut(s) 959
MspCI CTTAAG 1 cut(s) 74
MspI CCGG 3 cut(s) 105, 527, 1004
MspR9I CCNGG 2 cut(s) 27, 105
MunI CAATTG 1 cut(s) 890
Mva1269I GAATGC 2 cut(s) 313, 1150
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 188
NciI CCSGG 1 cut(s) 105
NcoI CCATGG 2 cut(s) 45, 1125
NdeII GATC 6 cut(s) 133, 151, 214, 321, 423, 604
NlaIII CATG 6 cut(s) 49, 67, 413, 430, 590, 1129
NlaIV GGNNCC 1 cut(s) 627
NmuCI GTSAC 3 cut(s) 143, 450, 1052
NspV TTCGAA 1 cut(s) 177
PciSI GCTCTTC 1 cut(s) 1089
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 268
PctI GAATGC 2 cut(s) 313, 1150
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 3 cut(s) 174, 611, 707
PflFI GACNNNGTC 1 cut(s) 575
PkrI GCNGC 8 cut(s) 313, 348, 630, 690, 774, 933, 958, 1116
Ppu21I YACGTR 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 1 cut(s) 583
PstI CTGCAG 1 cut(s) 774
PstNI CAGNNNCTG 2 cut(s) 608, 778
PsuI RGATCY 4 cut(s) 133, 214, 321, 604
PsyI GACNNNGTC 1 cut(s) 575
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 4 cut(s) 292, 522, 726, 1169
RsaNI GTAC 4 cut(s) 291, 521, 725, 1168
RseI CAYNNNNRTG 2 cut(s) 993, 1130
SapI GCTCTTC 1 cut(s) 1089
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 8 cut(s) 312, 347, 629, 689, 773, 932, 957, 1115
Sau3AI GATC 6 cut(s) 133, 151, 214, 321, 423, 604
Sau96I GGNCC 1 cut(s) 583
ScaI AGTACT 1 cut(s) 1169
ScrFI CCNGG 2 cut(s) 27, 105
SduI GDGCHC 2 cut(s) 105, 465
SfaNI GCATC 2 cut(s) 552, 931
SfcI CTRYAG 1 cut(s) 770
SfuI TTCGAA 1 cut(s) 177
SinI GGWCC 1 cut(s) 583
SmiMI CAYNNNNRTG 2 cut(s) 993, 1130
SmlI CTYRAG 3 cut(s) 74, 199, 1048
SmoI CTYRAG 3 cut(s) 74, 199, 1048
Sse9I AATT 4 cut(s) 680, 815, 874, 890
SsiI CCGC 2 cut(s) 288, 629
SspMI CTAG 3 cut(s) 794, 839, 911
StyD4I CCNGG 2 cut(s) 25, 103
StyI CCWWGG 4 cut(s) 45, 155, 315, 1125
TaaI ACNGT 2 cut(s) 143, 277
TaiI ACGT 3 cut(s) 120, 265, 649
TaqI TCGA 2 cut(s) 177, 579
TasI AATT 4 cut(s) 680, 815, 874, 890
TatI WGTACW 3 cut(s) 520, 724, 1167
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 3 cut(s) 174, 611, 707
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TseFI GTSAC 3 cut(s) 143, 450, 1052
TseI GCWGC 7 cut(s) 311, 346, 688, 772, 931, 956, 1114
Tsp45I GTSAC 3 cut(s) 143, 450, 1052
TspDTI ATGAA 4 cut(s) 69, 747, 1011, 1118
TspGWI ACGGA 1 cut(s) 606
Tth111I GACNNNGTC 1 cut(s) 575
Vha464I CTTAAG 1 cut(s) 74
VneI GTGCAC 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 583
XagI CCTNNNNNAGG 1 cut(s) 909
XapI RAATTY 2 cut(s) 815, 874
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 3 cut(s) 794, 839, 911
ZrmI AGTACT 1 cut(s) 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.