Rh5DG419700

Isovaleryl-CoA dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006675
Physical Location & Seq
Reverse (-)
66836400 .. 66842049
5650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG419700.1

Sequence Viewer

Length: 813 bp
ATGAAGCTTATTAGTGGAGAGCATGTAGGGGCTCTTGCAATGAGCGAGCCCAATTCTGGTTCTGATGTAGTTAGCATGAAGTGCAAAGCTGATCGAGCAGATGGGGGTTATATCTTAAATGGGAACAAGATGTGGTGCACCAATGGACCAGTTGCTCAAACATTGGTTGTTTATGCAAAAACGGACATAAATGCGGGCTCAAAGGGAATCACAGCGTTCATCATTGAGAAGGGGATGCCCGGATTCAGTACTGCCCAGAAATTAGACAAACTCGGCATGCGAGGAAGCGATACATGTGAGCTTGTCTTTGAGAATTGCTTTGTTCCGAATGAAAATGTTCTTGGCCAGGAAGGAAAAGGGGTCTATGTCATGATGTCAGGGTTAGATTTAGAGAGGCTTGTTTTAGCAGGTGGGCCCCTAGGTATTATGCAGGCATGTATGGATGTTGTCCTTCCTTATGTTCGACAGCGAGAGCAGTTTGGCCGTCCTATTGGAGAGTTTCAGCTTATACAGGGGAAAATTGCTGACATGTATACTTCTTTACAATCTTCAAGGGCCTATGTGTATTCTGTTGCAAGGGAATGTGACAATGGAAGAGTTGACCCAAAGGACTGTGCAGGAGTTATACTATCTACGGCTGAAAGAGCCACGCAGGTTGCTTTGCAGGCTATACAATGCTTAGGTGGTAATGGCTATGTAAATGAGTACCCAACTGGCCGTCTCCTTCGAGATGCCAAACTCTATGAGATTGGAGCAGGGACTAGTGAGATCAGAAGGATGATAATTGGTCGTGGGCTATTTAAGGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0003853 GO:0003995 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006139 GO:0006163 GO:0006195 GO:0006520 GO:0006551 GO:0006552 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008470 GO:0009056 GO:0009058 GO:0009063 GO:0009081 GO:0009083 GO:0009109 GO:0009117 GO:0009150 GO:0009154 GO:0009166 GO:0009259 GO:0009261 GO:0009987 GO:0016054 GO:0016491 GO:0016627 GO:0017076 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033869 GO:0033875 GO:0034031 GO:0034032 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035337 GO:0035383 GO:0035639 GO:0036094 GO:0036115 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046434 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0072521 GO:0072523 GO:0097159 GO:0097367 GO:1901135 GO:1901136 GO:1901265 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901568 GO:1901569 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1902189 GO:1902190 GO:1902192 GO:1902195 GO:1902196 GO:1902198
KEGG Pathways
Metabolic & Signaling
ko00280 ko01100 map00280 map01100
Pfam Domains
Protein Families
Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

270

Amino Acids

29.19

Weight (kDa)

6.61

Isoelectric Point (pI)

30.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-CoA_dh_M PF02770 11 - 106 5.8e-26 Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1 PF00441 118 - 266 3.3e-45 Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2 PF08028 135 - 249 5.2e-11 Acyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
Acc36I ACCTGC 2 cut(s) 398, 643
AccI GTMKAC 1 cut(s) 533
AciI CCGC 1 cut(s) 194
AcoI YGGCCR 3 cut(s) 343, 481, 715
AfaI GTAC 2 cut(s) 250, 707
AfiI CCNNNNNNNGG 1 cut(s) 56
AflIII ACRYGT 2 cut(s) 293, 528
AgsI TTSAA 1 cut(s) 552
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 1 cut(s) 345
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AluBI AGCT 4 cut(s) 7, 89, 301, 505
AluI AGCT 4 cut(s) 7, 89, 301, 505
Alw21I GWGCWC 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 725
Alw44I GTGCAC 1 cut(s) 136
AoxI GGCC 5 cut(s) 343, 413, 481, 555, 715
ApaI GGGCCC 1 cut(s) 417
ApaLI GTGCAC 1 cut(s) 136
Asp700I GAANNNNTTC 1 cut(s) 336
AspA2I CCTAGG 1 cut(s) 418
AspS9I GGNCC 4 cut(s) 146, 413, 414, 555
AsuC2I CCSGG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 146
AvrII CCTAGG 1 cut(s) 418
BaeGI GKGCMC 2 cut(s) 140, 417
BalI TGGCCA 1 cut(s) 345
BanII GRGCYC 4 cut(s) 34, 51, 200, 417
Bbv12I GWGCWC 1 cut(s) 140
BccI CCATC 1 cut(s) 95
BceAI ACGGC 3 cut(s) 468, 651, 702
BciT130I CCWGG 1 cut(s) 347
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 725
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 2 cut(s) 419, 762
BfuAI ACCTGC 2 cut(s) 398, 643
BlnI CCTAGG 1 cut(s) 418
BmcAI AGTACT 1 cut(s) 250
Bme1390I CCNGG 2 cut(s) 240, 347
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 4 cut(s) 146, 413, 414, 555
BmiI GGNNCC 2 cut(s) 415, 416
BmrFI CCNGG 2 cut(s) 240, 347
BmsI GCATC 2 cut(s) 225, 721
Bpu10I CCTNAGC 1 cut(s) 679
BpuMI CCSGG 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse1I ACTGG 2 cut(s) 149, 718
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 3 cut(s) 240, 448, 783
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMI GCAATG 1 cut(s) 45
BseNI ACTGG 2 cut(s) 149, 718
BseSI GKGCMC 2 cut(s) 140, 417
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 5 cut(s) 345, 415, 483, 557, 717
BsiHKAI GWGCWC 1 cut(s) 140
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 725
BsmBI CGTCTC 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 772
BsnI GGCC 5 cut(s) 345, 415, 483, 557, 717
Bsp120I GGGCCC 1 cut(s) 413
Bsp1286I GDGCHC 5 cut(s) 34, 51, 140, 200, 417
Bsp143I GATC 2 cut(s) 91, 768
BspACI CCGC 1 cut(s) 194
BspANI GGCC 5 cut(s) 345, 415, 483, 557, 717
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 415, 416
BspMI ACCTGC 2 cut(s) 398, 643
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 149, 718
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 2 cut(s) 91, 768
BssNAI GTATAC 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 1 cut(s) 614
Bst6I CTCTTC 1 cut(s) 589
BstAPI GCANNNNNTGC 1 cut(s) 81
BstC8I GCNNGC 5 cut(s) 47, 196, 278, 432, 666
BstDEI CTNAG 1 cut(s) 679
BstF5I GGATG 3 cut(s) 240, 448, 783
BstKTI GATC 2 cut(s) 94, 771
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 2 cut(s) 91, 768
BstMWI GCNNNNNNNGC 4 cut(s) 81, 95, 644, 665
BstNI CCWGG 1 cut(s) 347
BstNSI RCATGY 5 cut(s) 26, 280, 297, 438, 532
BstSCI CCNGG 2 cut(s) 238, 345
BstSLI GKGCMC 2 cut(s) 140, 417
BstZ17I GTATAC 1 cut(s) 534
BsuRI GGCC 5 cut(s) 345, 415, 483, 557, 717
BtsCI GGATG 3 cut(s) 240, 448, 783
BveI ACCTGC 2 cut(s) 398, 643
Cac8I GCNNGC 5 cut(s) 47, 196, 278, 432, 666
CciI TCATGA 1 cut(s) 369
Cfr13I GGNCC 4 cut(s) 146, 413, 414, 555
Csp6I GTAC 2 cut(s) 249, 706
CspCI CAANNNNNGTGG 2 cut(s) 637, 672
CviAII CATG 7 cut(s) 23, 76, 277, 294, 370, 435, 529
CviQI GTAC 2 cut(s) 249, 706
DdeI CTNAG 1 cut(s) 679
DpnI GATC 2 cut(s) 93, 770
DpnII GATC 2 cut(s) 91, 768
EaeI YGGCCR 3 cut(s) 343, 481, 715
Eam1104I CTCTTC 1 cut(s) 589
EarI CTCTTC 1 cut(s) 589
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 4 cut(s) 34, 51, 200, 417
Eco47I GGWCC 1 cut(s) 146
EcoO109I RGGNCCY 2 cut(s) 414, 555
EcoRII CCWGG 1 cut(s) 345
EcoT14I CCWWGG 1 cut(s) 418
EcoT38I GRGCYC 4 cut(s) 34, 51, 200, 417
ErhI CCWWGG 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 725
FaeI CATG 7 cut(s) 26, 79, 280, 297, 373, 438, 532
FaqI GGGAC 1 cut(s) 772
FatI CATG 7 cut(s) 22, 75, 276, 293, 369, 434, 528
FauI CCCGC 1 cut(s) 187
FblI GTMKAC 1 cut(s) 533
FokI GGATG 3 cut(s) 247, 455, 790
FriOI GRGCYC 4 cut(s) 34, 51, 200, 417
FspBI CTAG 2 cut(s) 419, 762
HaeIII GGCC 5 cut(s) 345, 415, 483, 557, 717
HapII CCGG 1 cut(s) 240
Hin1II CATG 7 cut(s) 26, 79, 280, 297, 373, 438, 532
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 207, 243
HpaII CCGG 1 cut(s) 240
Hpy166II GTNNAC 3 cut(s) 138, 534, 601
Hpy188I TCNGA 3 cut(s) 64, 327, 773
Hpy188III TCNNGA 2 cut(s) 370, 728
Hpy8I GTNNAC 3 cut(s) 138, 534, 601
HpyAV CCTTC 5 cut(s) 223, 344, 461, 734, 768
HpyCH4III ACNGT 1 cut(s) 614
HpyCH4V TGCA 8 cut(s) 38, 84, 138, 176, 430, 575, 617, 664
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 95, 644, 665
HpyF3I CTNAG 1 cut(s) 679
Hsp92II CATG 7 cut(s) 26, 79, 280, 297, 373, 438, 532
Kzo9I GATC 2 cut(s) 91, 768
LmnI GCTCC 2 cut(s) 752, 805
LweI GCATC 2 cut(s) 225, 721
MaeI CTAG 2 cut(s) 419, 762
MaeIII GTNAC 1 cut(s) 584
MalI GATC 2 cut(s) 93, 770
MboI GATC 2 cut(s) 91, 768
MboII GAAGA 2 cut(s) 540, 606
MhlI GDGCHC 5 cut(s) 34, 51, 140, 200, 417
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 5 cut(s) 52, 260, 313, 519, 783
MluNI TGGCCA 1 cut(s) 345
MnlI CCTC 2 cut(s) 275, 387
Mox20I TGGCCA 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 336
MscI TGGCCA 1 cut(s) 345
MseI TTAA 2 cut(s) 116, 801
Msp20I TGGCCA 1 cut(s) 345
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 240, 347
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 4 cut(s) 81, 95, 644, 665
NciI CCSGG 1 cut(s) 240
NdeII GATC 2 cut(s) 91, 768
NlaIII CATG 7 cut(s) 26, 79, 280, 297, 373, 438, 532
NlaIV GGNNCC 2 cut(s) 415, 416
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 584
NspI RCATGY 5 cut(s) 26, 280, 297, 438, 532
PaeI GCATGC 1 cut(s) 280
PagI TCATGA 1 cut(s) 369
PaqCI CACCTGC 1 cut(s) 398
PciI ACATGT 2 cut(s) 293, 528
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 207, 243
PscI ACATGT 2 cut(s) 293, 528
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 2 cut(s) 415, 416
PspOMI GGGCCC 1 cut(s) 413
PspPI GGNCC 4 cut(s) 146, 413, 414, 555
RsaI GTAC 2 cut(s) 250, 707
RsaNI GTAC 2 cut(s) 249, 706
SaqAI TTAA 2 cut(s) 116, 801
Sau3AI GATC 2 cut(s) 91, 768
Sau96I GGNCC 4 cut(s) 146, 413, 414, 555
ScaI AGTACT 1 cut(s) 250
ScrFI CCNGG 2 cut(s) 240, 347
SduI GDGCHC 5 cut(s) 34, 51, 140, 200, 417
SetI ASST 8 cut(s) 9, 91, 303, 412, 424, 507, 657, 685
SfaNI GCATC 2 cut(s) 225, 721
SinI GGWCC 1 cut(s) 146
SpeI ACTAGT 1 cut(s) 761
SphI GCATGC 1 cut(s) 280
Sse9I AATT 5 cut(s) 52, 260, 313, 519, 783
SsiI CCGC 1 cut(s) 194
SspMI CTAG 2 cut(s) 419, 762
StyD4I CCNGG 2 cut(s) 238, 345
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 614
TaqI TCGA 3 cut(s) 94, 463, 727
TasI AATT 5 cut(s) 52, 260, 313, 519, 783
TatI WGTACW 1 cut(s) 248
TfiI GAWTC 2 cut(s) 207, 243
Tru1I TTAA 2 cut(s) 116, 801
Tru9I TTAA 2 cut(s) 116, 801
TseFI GTSAC 1 cut(s) 584
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 4 cut(s) 17, 92, 208, 345
TspGWI ACGGA 1 cut(s) 197
VneI GTGCAC 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 146
XceI RCATGY 5 cut(s) 26, 280, 297, 438, 532
XmaJI CCTAGG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 533
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 2 cut(s) 419, 762
ZrmI AGTACT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.