Rh5BG404100

Isovaleryl-CoA dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006675
Physical Location & Seq
Reverse (-)
65503165 .. 65507211
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG404100.1

Sequence Viewer

Length: 930 bp
ATGGAAGAAATAAGCCGTGCTTCAGGATCTGTTGGCCTATCATATGGTGCCCATTCCAACCTGTGCATTAATCAATTGGTGAGGCATGGAAATCCTACTCAGAAAGAGAAGTATTTGCCAAAGCTTATTAGTGGAGAGCATGTAGGGGCTCTTGCAATGAGCGAGCCCAATTCTGGTTCTGACGTAGTTAGCATGAAGTGCAAAGCTGATCGAGCAGATGGGGGTTATATCTTAAATGGGAACAAGATGTGGTGCACCAATGGCCCAGTTGCTCAAACATTGGTTGTTTATGCAAAAACGGACATAAATGCGGGCTCAAAGGGAATCACAGCGTTCATCATTGAGAAGGGGATACCCGGATTCAGTACTGCCCAGAAATTAGACAAACTCGGCATGCGAGGAAGCGATACATGTGAGCTTGTCTTTGAGAATTGCTTTGTTTCGAATGAAAATGTTCTTGGCCAGGAAGGAAAAGGAGTCTATGTCATGATGTCAGGGTTAGATTTAGAGAGGCTTGTTTTAGCAGGTGGGCCCCTAGGTATTATGCAGGCATGTATGGATGTTGTCCTTCCTTATGTTCGACAGCGAGAGCAGTTTGGCCGTCCTATTGGAGAGTTTCAGCTTATACAGGGGAAAATTGCTGACATGTATACTTCTTTACAATCTTCAAGGGCCTATGTGTATTCTGTTGCAAGGGAATGTGACAATGGAAGAGTTGACCCAAAGGACTGTGCAGGAGTTATACTATCTACGGCTGAAAGAGCCACGCAGGTTGCTTTGCAGGCTATACAATGCTTAGGTGGTAATGGCTATGTAAATGAGTACCCAACTGGCCGTCTCCTTCGAGATGCCAAACTCTATGAGATTGGAGCAGGGACTAGTGAGATCAGAAGGATGATAATTGGTCGTGGGCTATTTAAGGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0003853 GO:0003995 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006139 GO:0006163 GO:0006195 GO:0006520 GO:0006551 GO:0006552 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008470 GO:0009056 GO:0009058 GO:0009063 GO:0009081 GO:0009083 GO:0009109 GO:0009117 GO:0009150 GO:0009154 GO:0009166 GO:0009259 GO:0009261 GO:0009987 GO:0016054 GO:0016491 GO:0016627 GO:0017076 GO:0018130 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033869 GO:0033875 GO:0034031 GO:0034032 GO:0034034 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0035337 GO:0035383 GO:0035639 GO:0036094 GO:0036115 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044273 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046395 GO:0046434 GO:0046483 GO:0046700 GO:0051186 GO:0051187 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0072521 GO:0072523 GO:0097159 GO:0097367 GO:1901135 GO:1901136 GO:1901265 GO:1901292 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901568 GO:1901569 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1902189 GO:1902190 GO:1902192 GO:1902195 GO:1902196 GO:1902198
KEGG Pathways
Metabolic & Signaling
ko00280 ko01100 map00280 map01100
Pfam Domains
Protein Families
Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

309

Amino Acids

33.39

Weight (kDa)

7.49

Isoelectric Point (pI)

27.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-CoA_dh_N PF02771 1 - 46 2.5e-12 Acyl-CoA dehydrogenase, N-terminal domain
Acyl-CoA_dh_M PF02770 50 - 145 6.5e-25 Acyl-CoA dehydrogenase, middle domain
Acyl-CoA_dh_1 PF00441 157 - 305 4.7e-45 Acyl-CoA dehydrogenase, C-terminal domain
Acyl-CoA_dh_2 PF08028 174 - 288 7e-11 Acyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 515
Acc36I ACCTGC 2 cut(s) 515, 760
AccB1I GGYRCC 1 cut(s) 47
AccI GTMKAC 1 cut(s) 650
AciI CCGC 1 cut(s) 311
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 3 cut(s) 460, 598, 832
AcuI CTGAAG 1 cut(s) 6
AfaI GTAC 2 cut(s) 367, 824
AfiI CCNNNNNNNGG 1 cut(s) 173
AflIII ACRYGT 2 cut(s) 410, 645
AgsI TTSAA 1 cut(s) 669
AhlI ACTAGT 1 cut(s) 878
AjnI CCWGG 1 cut(s) 462
AjuI GAANNNNNNNTTGG 2 cut(s) 441, 473
AluBI AGCT 4 cut(s) 124, 206, 418, 622
AluI AGCT 4 cut(s) 124, 206, 418, 622
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 842
Alw44I GTGCAC 1 cut(s) 253
AlwI GGATC 1 cut(s) 34
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 7 cut(s) 34, 262, 460, 530, 598, 672, 832
ApaI GGGCCC 1 cut(s) 534
ApaLI GTGCAC 1 cut(s) 253
AseI ATTAAT 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 453
AspA2I CCTAGG 1 cut(s) 535
AspS9I GGNCC 4 cut(s) 263, 530, 531, 672
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 443
AvrII CCTAGG 1 cut(s) 535
BaeGI GKGCMC 3 cut(s) 52, 257, 534
BalI TGGCCA 1 cut(s) 462
BanI GGYRCC 1 cut(s) 47
BanII GRGCYC 4 cut(s) 151, 168, 317, 534
Bbv12I GWGCWC 1 cut(s) 257
BccI CCATC 1 cut(s) 212
BceAI ACGGC 3 cut(s) 585, 768, 819
BciT130I CCWGG 1 cut(s) 464
BciVI GTATCC 1 cut(s) 345
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 842
BcuI ACTAGT 1 cut(s) 878
BfaI CTAG 2 cut(s) 536, 879
BfuAI ACCTGC 2 cut(s) 515, 760
BfuI GTATCC 1 cut(s) 345
BlnI CCTAGG 1 cut(s) 535
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 357, 464
BmgT120I GGNCC 4 cut(s) 263, 530, 531, 672
BmiI GGNNCC 3 cut(s) 49, 532, 533
BmrFI CCNGG 2 cut(s) 357, 464
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 1 cut(s) 838
BmuI ACTGGG 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 796
Bpu14I TTCGAA 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 535
BsaXI ACNNNNNCTCC 4 cut(s) 126, 156, 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 266, 835
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 535
BseGI GGATG 2 cut(s) 565, 900
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 2 cut(s) 266, 835
BseSI GKGCMC 3 cut(s) 52, 257, 534
BsgI GTGCAG 1 cut(s) 753
BshFI GGCC 7 cut(s) 36, 264, 462, 532, 600, 674, 834
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 257
BsiSI CCGG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 1 cut(s) 173
BsmAI GTCTC 1 cut(s) 842
BsmBI CGTCTC 1 cut(s) 842
BsmFI GGGAC 1 cut(s) 889
BsnI GGCC 7 cut(s) 36, 264, 462, 532, 600, 674, 834
Bsp119I TTCGAA 1 cut(s) 443
Bsp120I GGGCCC 1 cut(s) 530
Bsp1286I GDGCHC 6 cut(s) 52, 151, 168, 257, 317, 534
Bsp143I GATC 3 cut(s) 26, 208, 885
BspACI CCGC 1 cut(s) 311
BspANI GGCC 7 cut(s) 36, 264, 462, 532, 600, 674, 834
BspCNI CTCAG 1 cut(s) 112
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 3 cut(s) 49, 532, 533
BspMI ACCTGC 2 cut(s) 515, 760
BspPI GGATC 1 cut(s) 34
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 162
BsrI ACTGG 2 cut(s) 266, 835
BssECI CCNNGG 1 cut(s) 535
BssMI GATC 3 cut(s) 26, 208, 885
BssNAI GTATAC 1 cut(s) 651
BssT1I CCWWGG 1 cut(s) 535
Bst1107I GTATAC 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 1 cut(s) 731
Bst6I CTCTTC 1 cut(s) 706
BstAPI GCANNNNNTGC 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 5 cut(s) 164, 313, 395, 549, 783
BstDEI CTNAG 2 cut(s) 99, 796
BstF5I GGATG 2 cut(s) 565, 900
BstKTI GATC 3 cut(s) 29, 211, 888
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 3 cut(s) 26, 208, 885
BstMWI GCNNNNNNNGC 5 cut(s) 198, 212, 261, 761, 782
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 5 cut(s) 143, 397, 414, 555, 649
BstSCI CCNGG 2 cut(s) 355, 462
BstSLI GKGCMC 3 cut(s) 52, 257, 534
BstX2I RGATCY 1 cut(s) 26
BstYI RGATCY 1 cut(s) 26
BstZ17I GTATAC 1 cut(s) 651
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 7 cut(s) 36, 264, 462, 532, 600, 674, 834
BtsCI GGATG 2 cut(s) 565, 900
BveI ACCTGC 2 cut(s) 515, 760
Cac8I GCNNGC 5 cut(s) 164, 313, 395, 549, 783
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 1 cut(s) 486
Cfr13I GGNCC 4 cut(s) 263, 530, 531, 672
Csp6I GTAC 2 cut(s) 366, 823
CspCI CAANNNNNGTGG 2 cut(s) 754, 789
CviAII CATG 8 cut(s) 86, 140, 193, 394, 411, 487, 552, 646
CviQI GTAC 2 cut(s) 366, 823
DdeI CTNAG 2 cut(s) 99, 796
DpnI GATC 3 cut(s) 28, 210, 887
DpnII GATC 3 cut(s) 26, 208, 885
EaeI YGGCCR 3 cut(s) 460, 598, 832
Eam1104I CTCTTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 706
Eco130I CCWWGG 1 cut(s) 535
Eco24I GRGCYC 4 cut(s) 151, 168, 317, 534
Eco57I CTGAAG 1 cut(s) 6
EcoO109I RGGNCCY 2 cut(s) 531, 672
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 535
EcoT38I GRGCYC 4 cut(s) 151, 168, 317, 534
ErhI CCWWGG 1 cut(s) 535
Esp3I CGTCTC 1 cut(s) 842
FaeI CATG 8 cut(s) 89, 143, 196, 397, 414, 490, 555, 649
FalI AAGNNNNNCTT 1 cut(s) 36
FaqI GGGAC 1 cut(s) 889
FatI CATG 8 cut(s) 85, 139, 192, 393, 410, 486, 551, 645
FauI CCCGC 1 cut(s) 304
FauNDI CATATG 1 cut(s) 43
FblI GTMKAC 1 cut(s) 650
FokI GGATG 2 cut(s) 572, 907
FriOI GRGCYC 4 cut(s) 151, 168, 317, 534
FspBI CTAG 2 cut(s) 536, 879
HaeIII GGCC 7 cut(s) 36, 264, 462, 532, 600, 674, 834
HapII CCGG 1 cut(s) 357
Hin1II CATG 8 cut(s) 89, 143, 196, 397, 414, 490, 555, 649
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 3 cut(s) 324, 360, 477
HpaII CCGG 1 cut(s) 357
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 3 cut(s) 255, 651, 718
Hpy188I TCNGA 3 cut(s) 102, 181, 890
Hpy188III TCNNGA 3 cut(s) 24, 487, 845
Hpy8I GTNNAC 3 cut(s) 255, 651, 718
HpyAV CCTTC 5 cut(s) 340, 461, 578, 851, 885
HpyCH4III ACNGT 1 cut(s) 731
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 9 cut(s) 66, 155, 201, 255, 293, 547, 692, 734, 781
HpyF10VI GCNNNNNNNGC 5 cut(s) 198, 212, 261, 761, 782
HpyF3I CTNAG 2 cut(s) 99, 796
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 8 cut(s) 89, 143, 196, 397, 414, 490, 555, 649
Kzo9I GATC 3 cut(s) 26, 208, 885
LmnI GCTCC 2 cut(s) 869, 922
LweI GCATC 1 cut(s) 838
MaeI CTAG 2 cut(s) 536, 879
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 1 cut(s) 701
MalI GATC 3 cut(s) 28, 210, 887
MboI GATC 3 cut(s) 26, 208, 885
MboII GAAGA 3 cut(s) 17, 657, 723
MfeI CAATTG 1 cut(s) 74
MflI RGATCY 1 cut(s) 26
MhlI GDGCHC 6 cut(s) 52, 151, 168, 257, 317, 534
MlsI TGGCCA 1 cut(s) 462
MluCI AATT 6 cut(s) 74, 169, 377, 430, 636, 900
MluNI TGGCCA 1 cut(s) 462
MlyI GAGTC 1 cut(s) 486
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 3 cut(s) 75, 392, 504
Mox20I TGGCCA 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 1 cut(s) 462
MseI TTAA 3 cut(s) 69, 233, 918
Msp20I TGGCCA 1 cut(s) 462
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 2 cut(s) 357, 464
MunI CAATTG 1 cut(s) 74
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 5 cut(s) 198, 212, 261, 761, 782
NciI CCSGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 43
NdeII GATC 3 cut(s) 26, 208, 885
NlaIII CATG 8 cut(s) 89, 143, 196, 397, 414, 490, 555, 649
NlaIV GGNNCC 3 cut(s) 49, 532, 533
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 701
NspI RCATGY 5 cut(s) 143, 397, 414, 555, 649
NspV TTCGAA 1 cut(s) 443
PaeI GCATGC 1 cut(s) 397
PagI TCATGA 1 cut(s) 486
PaqCI CACCTGC 1 cut(s) 515
PciI ACATGT 2 cut(s) 410, 645
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 2 cut(s) 324, 360
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PscI ACATGT 2 cut(s) 410, 645
PshBI ATTAAT 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 3 cut(s) 49, 532, 533
PspOMI GGGCCC 1 cut(s) 530
PspPI GGNCC 4 cut(s) 263, 530, 531, 672
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 26
RsaI GTAC 2 cut(s) 367, 824
RsaNI GTAC 2 cut(s) 366, 823
SaqAI TTAA 3 cut(s) 69, 233, 918
Sau3AI GATC 3 cut(s) 26, 208, 885
Sau96I GGNCC 4 cut(s) 263, 530, 531, 672
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 2 cut(s) 357, 464
SduI GDGCHC 6 cut(s) 52, 151, 168, 257, 317, 534
SfaNI GCATC 1 cut(s) 838
SfuI TTCGAA 1 cut(s) 443
SpeI ACTAGT 1 cut(s) 878
SphI GCATGC 1 cut(s) 397
Sse9I AATT 6 cut(s) 74, 169, 377, 430, 636, 900
SsiI CCGC 1 cut(s) 311
SspMI CTAG 2 cut(s) 536, 879
StyD4I CCNGG 2 cut(s) 355, 462
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 1 cut(s) 731
TaiI ACGT 1 cut(s) 186
TaqI TCGA 4 cut(s) 211, 443, 580, 844
TasI AATT 6 cut(s) 74, 169, 377, 430, 636, 900
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 2 cut(s) 324, 360
Tru1I TTAA 3 cut(s) 69, 233, 918
Tru9I TTAA 3 cut(s) 69, 233, 918
TseFI GTSAC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 701
TspDTI ATGAA 3 cut(s) 209, 325, 462
TspGWI ACGGA 1 cut(s) 314
VneI GTGCAC 1 cut(s) 253
VspI ATTAAT 1 cut(s) 69
XceI RCATGY 5 cut(s) 143, 397, 414, 555, 649
XmaJI CCTAGG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 2 cut(s) 536, 879
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.