FvH4_3g37140

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011956
Physical Location & Seq
Forward (+)
31825253 .. 31828117
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37140.t2

Sequence Viewer

Length: 1173 bp
ATGGAGCATGAGAAGGTGCCCTTATCTAGAACGGAGAACCTGCACGAAATTGAAATCCCTATAGTCTCTGAAAGTATCGATGTTCTTCATGTGCCACAAAAGTTACCCTGCTCTTCCATCTGCGCTTTCTCCAAGAAAGAAAGCAGTAGTATGGATTCAAAACAACGTTCAAAATCTGCAACTAAACTTGGAGTACTCATAATTGTCTATCTGTTGTTTATGGTTGTTGAAATTGTTGGTGGTGTAAAATCCAACAGCCTGGCAGTACTAACAGATGCAGCCCACATGCTTACCGATGTTAGTGGATTTGCTATTGCTCTTTTCGCCATAATTGCGTCAGGTTGGGAGGCAACATCTGACCAGTCTTTTGGGTATCACCGTCTTGAGGTTTTGAGTGCGCTTCTTTCGGTACAGCTGATATGGGTGGTCGCTGTGATCTTGATCTATGAAGCAGTCGACAGAATGCTTCATGAGCATGCAAAGGTAAATGGGGTTCTCATGTTTGCAGTTGCAGCATTTGGATTTTTGGTTAATTTTATCATGGTAATGTGGCTCGGCCATGATCACACTCATCATCATCATCATCATCATCAGCACCATCATACCTGTGGAGACTTGGATTATCATGATGATCATGGTCATAATCATGATCATGATCATGGCAAGGAAGAAGCATGTACAATAACTGAAGACGATGAAACTAGCCTGGTGTCAAGTGCTCAAGTAAAGACTAAAATATTAAACATAAATCTCGAAGGAGCTTACCTGCATGTTATGGCTGACATGATTCAGTCGATTGGAGTAATGATAGCCGGAGCCATCATCTGGGCGAAGCCAGATTGGTTAGTTGTTGATCTGATCTGCACTCTCATCTTTTCTGTTTTTGCTGTAAGCACAACTATACCGATGCTCAGAAATACATATGGCATATTGATGCAGAGAACACCAAGTGAGATTGATATCAACAAGCTAGAGAAAGGTCTCAAGAGCATCAAAGGAGTTCAGGATGTTCATGACCTACATGTTTGGTCACTGACAGTTGGAAAAATGGTATTGTCGTGCCATGTCACCGCGGATCCTACGGTAGGCTCAAGTCAGATAATTGATGAAATTAGGGATTTTTGTGAAAGAACATACAGAATACATGATGTTACTGTACAGATTGAACAGTAG

Protein Analysis

391

Amino Acids

43.5

Weight (kDa)

5.77

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_efflux PF01545 65 - 278 8e-36 Cation efflux transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011956)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29410
fragaria_vesca FvH4_3g37140 FvH4_3g37140 FvH4_3g37140
malus_domestica MD03G1084800.v1.1
prunus_persica Prupe.6G068100_v2.0.a1 Prupe.6G068100_v2.0.a1
pyrus_communis pycom03g06700 pycom11g07760
rosa_chinensis RchiOBHm_Chr5g0066861
rosa_laevigata RLG00000035883
rosa_multiflora Rmu_sc0005860.1_g000002
rosa_roxburghii Rroxscaffold_1G00014260
rosa_rugosa Rorug05G0379000
rosa_samantha Rh5AG438700 Rh5BG455600 Rh5CG477700 Rh5DG470400
rosa_wichuraiana Rw5G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 48, 774
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 825
AccI GTMKAC 1 cut(s) 456
AccII CGCG 1 cut(s) 1073
AciI CCGC 2 cut(s) 1071, 1073
AclI AACGTT 1 cut(s) 166
AclWI GGATC 2 cut(s) 1070, 1083
AcoI YGGCCR 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 708
AdeI CACNNNGTG 1 cut(s) 950
AfaI GTAC 5 cut(s) 195, 267, 411, 679, 1158
AfiI CCNNNNNNNGG 3 cut(s) 385, 825, 1085
AflIII ACRYGT 1 cut(s) 1021
AgsI TTSAA 5 cut(s) 53, 159, 171, 230, 1166
AjnI CCWGG 2 cut(s) 258, 705
AluBI AGCT 3 cut(s) 415, 761, 970
AluI AGCT 3 cut(s) 415, 761, 970
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 3 cut(s) 70, 606, 986
AlwI GGATC 2 cut(s) 1070, 1083
AoxI GGCC 1 cut(s) 556
ApeKI GCWGC 2 cut(s) 278, 512
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 2 cut(s) 125, 400
AsuHPI GGTGA 2 cut(s) 368, 1060
BaeGI GKGCMC 1 cut(s) 21
BamHI GGATCC 1 cut(s) 1075
BanI GGYRCC 1 cut(s) 16
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 2 cut(s) 290, 524
BccI CCATC 3 cut(s) 125, 606, 827
BciT130I CCWGG 2 cut(s) 260, 707
BclI TGATCA 4 cut(s) 562, 631, 649, 655
BcoDI GTCTC 3 cut(s) 70, 606, 986
BfaI CTAG 3 cut(s) 27, 702, 971
BfmI CTRYAG 1 cut(s) 60
BfuAI ACCTGC 2 cut(s) 48, 774
BisI GCNGC 2 cut(s) 279, 513
BlsI GCNGC 2 cut(s) 280, 514
BmcAI AGTACT 2 cut(s) 195, 267
Bme1390I CCNGG 2 cut(s) 260, 707
BmiI GGNNCC 3 cut(s) 18, 817, 1077
BmrFI CCNGG 2 cut(s) 260, 707
BmsI GCATC 4 cut(s) 265, 897, 924, 999
BpiI GAAGAC 1 cut(s) 696
BpuEI CTTGAG 4 cut(s) 404, 705, 968, 1075
Bsa29I ATCGAT 1 cut(s) 78
BsaBI GATNNNNATC 3 cut(s) 440, 654, 959
BsaI GGTCTC 1 cut(s) 986
BsaJI CCNNGG 1 cut(s) 1071
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 825, 1085
Bse1I ACTGG 1 cut(s) 361
Bse8I GATNNNNATC 3 cut(s) 440, 654, 959
BseBI CCWGG 2 cut(s) 260, 707
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 1071
BseGI GGATG 1 cut(s) 1012
BseJI GATNNNNATC 3 cut(s) 440, 654, 959
BseLI CCNNNNNNNGG 3 cut(s) 385, 825, 1085
BseMII CTCAG 1 cut(s) 925
BseNI ACTGG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 2 cut(s) 290, 524
BsgI GTGCAG 2 cut(s) 26, 847
Bsh1236I CGCG 1 cut(s) 1073
BshFI GGCC 1 cut(s) 558
BshNI GGYRCC 1 cut(s) 16
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 721
BsiSI CCGG 1 cut(s) 813
BslI CCNNNNNNNGG 3 cut(s) 385, 825, 1085
BsmAI GTCTC 3 cut(s) 70, 606, 986
BsmI GAATGC 1 cut(s) 468
BsnI GGCC 1 cut(s) 558
Bso31I GGTCTC 1 cut(s) 986
Bsp1286I GDGCHC 2 cut(s) 21, 721
Bsp1407I TGTACA 2 cut(s) 677, 1156
Bsp143I GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
BspACI CCGC 2 cut(s) 1071, 1073
BspANI GGCC 1 cut(s) 558
BspCNI CTCAG 1 cut(s) 924
BspDI ATCGAT 1 cut(s) 78
BspFNI CGCG 1 cut(s) 1073
BspHI TCATGA 5 cut(s) 469, 625, 646, 652, 1012
BspLI GGNNCC 3 cut(s) 18, 817, 1077
BspMI ACCTGC 2 cut(s) 48, 774
BspPI GGATC 2 cut(s) 1070, 1083
BspQI GCTCTTC 1 cut(s) 118
BspT107I GGYRCC 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 986
BsrGI TGTACA 2 cut(s) 677, 1156
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 1 cut(s) 1071
BssMI GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
Bst2UI CCWGG 2 cut(s) 260, 707
Bst4CI ACNGT 5 cut(s) 380, 1039, 1084, 1156, 1170
Bst6I CTCTTC 1 cut(s) 118
BstAUI TGTACA 2 cut(s) 677, 1156
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 1 cut(s) 911
BstDSI CCRYGG 1 cut(s) 1071
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 1 cut(s) 1012
BstFNI CGCG 1 cut(s) 1073
BstHHI GCGC 2 cut(s) 125, 400
BstKTI GATC 9 cut(s) 438, 444, 565, 634, 652, 658, 856, 861, 1078
BstMAI GTCTC 3 cut(s) 70, 606, 986
BstMBI GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
BstMWI GCNNNNNNNGC 4 cut(s) 323, 332, 472, 512
BstNI CCWGG 2 cut(s) 260, 707
BstNSI RCATGY 5 cut(s) 289, 479, 678, 773, 1025
BstSCI CCNGG 2 cut(s) 258, 705
BstSFI CTRYAG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 21
BstUI CGCG 1 cut(s) 1073
BstV1I GCAGC 2 cut(s) 290, 524
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 1075
BstXI CCANNNNNNTGG 2 cut(s) 259, 368
BstYI RGATCY 1 cut(s) 1075
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 1 cut(s) 558
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 1071
BtsCI GGATG 1 cut(s) 1012
BtsIMutI CAGTG 1 cut(s) 1031
BveI ACCTGC 2 cut(s) 48, 774
Cac8I GCNNGC 1 cut(s) 477
CciI TCATGA 5 cut(s) 469, 625, 646, 652, 1012
CfoI GCGC 2 cut(s) 125, 400
Cfr42I CCGCGG 1 cut(s) 1074
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 1 cut(s) 324
Csp6I GTAC 5 cut(s) 194, 266, 410, 678, 1157
CviQI GTAC 5 cut(s) 194, 266, 410, 678, 1157
DdeI CTNAG 1 cut(s) 911
DpnI GATC 9 cut(s) 437, 443, 564, 633, 651, 657, 855, 860, 1077
DpnII GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
DraIII CACNNNGTG 1 cut(s) 950
EaeI YGGCCR 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 986
Eco32I GATATC 1 cut(s) 961
Eco57I CTGAAG 1 cut(s) 708
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoRII CCWGG 2 cut(s) 258, 705
EcoRV GATATC 1 cut(s) 961
FalI AAGNNNNNCTT 1 cut(s) 37
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 4 cut(s) 562, 631, 649, 655
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 2 cut(s) 279, 513
FokI GGATG 1 cut(s) 1019
Fsp4HI GCNGC 2 cut(s) 279, 513
FspBI CTAG 3 cut(s) 27, 702, 971
GlaI GCGC 2 cut(s) 124, 399
GluI GCNGC 2 cut(s) 279, 513
HaeIII GGCC 1 cut(s) 558
HapII CCGG 1 cut(s) 813
HgaI GACGC 1 cut(s) 324
HhaI GCGC 2 cut(s) 125, 400
Hin6I GCGC 2 cut(s) 123, 398
HinP1I GCGC 2 cut(s) 123, 398
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 2 cut(s) 155, 787
HpaII CCGG 1 cut(s) 813
HphI GGTGA 2 cut(s) 368, 1060
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 5 cut(s) 70, 358, 858, 914, 1098
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 2 cut(s) 7, 749
HpyCH4III ACNGT 5 cut(s) 380, 1039, 1084, 1156, 1170
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 9 cut(s) 43, 179, 278, 479, 506, 512, 769, 864, 937
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 332, 472, 512
HpyF3I CTNAG 1 cut(s) 911
HpySE526I ACGT 1 cut(s) 166
HspAI GCGC 2 cut(s) 123, 398
Ksp22I TGATCA 4 cut(s) 562, 631, 649, 655
KspI CCGCGG 1 cut(s) 1074
Kzo9I GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
LguI GCTCTTC 1 cut(s) 118
LmnI GCTCC 3 cut(s) 4, 758, 815
Lsp1109I GCAGC 2 cut(s) 290, 524
LweI GCATC 4 cut(s) 265, 897, 924, 999
MaeI CTAG 3 cut(s) 27, 702, 971
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 4 cut(s) 102, 1029, 1066, 1150
MalI GATC 9 cut(s) 437, 443, 564, 633, 651, 657, 855, 860, 1077
MboI GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
MboII GAAGA 4 cut(s) 77, 105, 680, 701
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 2 cut(s) 21, 721
MluCI AATT 7 cut(s) 48, 201, 231, 330, 532, 1101, 1110
MmeI TCCRAC 2 cut(s) 276, 1021
MnlI CCTC 2 cut(s) 340, 379
MseI TTAA 2 cut(s) 531, 740
MslI CAYNNNNRTG 7 cut(s) 474, 545, 606, 645, 651, 657, 932
MspA1I CMGCKG 2 cut(s) 415, 1073
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 2 cut(s) 260, 707
Mva1269I GAATGC 1 cut(s) 468
MvaI CCWGG 2 cut(s) 260, 707
MvnI CGCG 1 cut(s) 1073
MwoI GCNNNNNNNGC 4 cut(s) 323, 332, 472, 512
NdeI CATATG 1 cut(s) 922
NdeII GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
NlaIV GGNNCC 3 cut(s) 18, 817, 1077
NmeAIII GCCGAG 1 cut(s) 534
NmuCI GTSAC 2 cut(s) 1029, 1066
NspI RCATGY 5 cut(s) 289, 479, 678, 773, 1025
PaeI GCATGC 1 cut(s) 479
PagI TCATGA 5 cut(s) 469, 625, 646, 652, 1012
PciI ACATGT 1 cut(s) 1021
PciSI GCTCTTC 1 cut(s) 118
PctI GAATGC 1 cut(s) 468
PfeI GAWTC 2 cut(s) 155, 787
PflMI CCANNNNNTGG 1 cut(s) 825
PkrI GCNGC 2 cut(s) 280, 514
PscI ACATGT 1 cut(s) 1021
Psp1406I AACGTT 1 cut(s) 166
Psp6I CCWGG 2 cut(s) 258, 705
PspGI CCWGG 2 cut(s) 258, 705
PspN4I GGNNCC 3 cut(s) 18, 817, 1077
PsrI GAACNNNNNNTAC 2 cut(s) 477, 509
PsuI RGATCY 1 cut(s) 1075
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 5 cut(s) 195, 267, 411, 679, 1158
RsaNI GTAC 5 cut(s) 194, 266, 410, 678, 1157
RseI CAYNNNNRTG 7 cut(s) 474, 545, 606, 645, 651, 657, 932
SacII CCGCGG 1 cut(s) 1074
SalI GTCGAC 1 cut(s) 455
SapI GCTCTTC 1 cut(s) 118
SaqAI TTAA 2 cut(s) 531, 740
SatI GCNGC 2 cut(s) 279, 513
Sau3AI GATC 9 cut(s) 435, 441, 562, 631, 649, 655, 853, 858, 1075
ScaI AGTACT 2 cut(s) 195, 267
ScrFI CCNGG 2 cut(s) 260, 707
SduI GDGCHC 2 cut(s) 21, 721
SfaNI GCATC 4 cut(s) 265, 897, 924, 999
SfcI CTRYAG 1 cut(s) 60
Sfr303I CCGCGG 1 cut(s) 1074
SgrBI CCGCGG 1 cut(s) 1074
SmiMI CAYNNNNRTG 7 cut(s) 474, 545, 606, 645, 651, 657, 932
SmlI CTYRAG 4 cut(s) 383, 720, 983, 1090
SmoI CTYRAG 4 cut(s) 383, 720, 983, 1090
SphI GCATGC 1 cut(s) 479
Sse9I AATT 7 cut(s) 48, 201, 231, 330, 532, 1101, 1110
SsiI CCGC 2 cut(s) 1071, 1073
SspI AATATT 1 cut(s) 738
SspMI CTAG 3 cut(s) 27, 702, 971
StyD4I CCNGG 2 cut(s) 258, 705
TaaI ACNGT 5 cut(s) 380, 1039, 1084, 1156, 1170
TaiI ACGT 1 cut(s) 169
TaqI TCGA 4 cut(s) 78, 456, 753, 794
TasI AATT 7 cut(s) 48, 201, 231, 330, 532, 1101, 1110
TatI WGTACW 4 cut(s) 193, 265, 677, 1156
TfiI GAWTC 2 cut(s) 155, 787
Tru1I TTAA 2 cut(s) 531, 740
Tru9I TTAA 2 cut(s) 531, 740
TscAI CASTG 1 cut(s) 1038
TseFI GTSAC 2 cut(s) 1029, 1066
TseI GCWGC 2 cut(s) 278, 512
Tsp45I GTSAC 2 cut(s) 1029, 1066
TspDTI ATGAA 6 cut(s) 77, 458, 462, 711, 1001, 1122
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 1038
Van91I CCANNNNNTGG 1 cut(s) 825
XagI CCTNNNNNAGG 1 cut(s) 1083
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 5 cut(s) 289, 479, 678, 773, 1025
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XmiI GTMKAC 1 cut(s) 456
XspI CTAG 3 cut(s) 27, 702, 971
ZrmI AGTACT 2 cut(s) 195, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.