Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011956

Physical Location & Seq

Genomic Coordinates Reverse (-)

6825770 .. 6828414

Gene Structure 2645 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1084800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGGAACATGAGAAGGTACCCTTTACGAGAATAGAGTCTCAGGAAATTGAGATCCCAATAGTCTCTGAAGGTATTGATGATCTTCCTGTGCCACCACAGGTGCCTCGCTCCTTGTGTGTCTGTGTTTTCTCCAAAAACGAGCACTGTAGTTTGGATTCAAAACAGCGGTCAAAGTCAGCTACAAAACTTTCTGTACTCGTTGTCGCATCTCTCATGTTTATGGTTGTTGAAATGATTGGTGGTGTCAAAGCCAACAGCCTTGCAGTACTCACAGATGCAGCCCACTTGCTCACTGATGTTGTTGGATTCTCCATTGCTCTTTTCACAGTATTGGCTTCAGGTTGGGAGGCAACATCGTACCAGTCTTTCGGATATCACCGTCTTGAGGTTTTGAGTGCCCTTTTCTCTGTGCAGCTCATATGGCTGGTCTCTGGGATCTTGATCTATGAGGCAGTTGACAGGATCGTTCACACTAAGGAAAAGGTGAACGGGTTACTCATGTTTTCAGTTGCAGCACTTGGATTTCTTGTCAACTTAATCATGGTCTTGTGGCTTGGTCACGACCACACCCATCATGCATGTGGAGGCTTAGGTCACGATCATCATCATGTTCATACTCACGATCATCATCATGGTCATAATCACAATCATCATCATGGTCATGATTATCAGGAGGAAGAAAGTGCAATAATGGAGGACGATAAAACGAGTCTGGTGTCATCAACATCTCCAGAAAAGACTAAAATATTAAACATAAATATCCAAGGAGCTTACTTGCATGTCATCGCCGATATGATTCAGTCCGTTGGGGTGATGGTCGCTGGAGGCATCATATGGGCAAGGCCAGATTGGTTAGTAGTTGATCTCATCTGCACACTCATATTTTCTGTTTTTGCTGTCAGCACAACTATATCGATGCTTAAAAATATATATGGCATATTGATGGAGAGAACACCAAGCGAGATTGATATCGCCGGACTAGAAAAAGGCATCAAGCGCATCAAAGGAGTTCGGGATGTTCACGACCTACACGTTTGGGCGCTAACGGTTGGAAAAACCGTGTTGTCTTGCCATGTAACGTCCGAGCCTGCCGTAAGCTCTAGTCAGATAATTGACAAGATTAGGGATTACTGTGAAAGTACTTACAGAATACATCATGTAACCATACAGATTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010043 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

393

Amino Acids

43.48

Weight (kDa)

6.02

Isoelectric Point (pI)

38.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	65 - 315	1.5e-34	Cation efflux transmembrane domain
ZT_dimer	PF16916	319 - 392	5.4e-14	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29410
fragaria_vesca	FvH4_3g37140 FvH4_3g37140 FvH4_3g37140
malus_domestica	MD03G1084800.v1.1
prunus_persica	Prupe.6G068100_v2.0.a1 Prupe.6G068100_v2.0.a1
pyrus_communis	pycom03g06700 pycom11g07760
rosa_chinensis	RchiOBHm_Chr5g0066861
rosa_laevigata	RLG00000035883
rosa_multiflora	Rmu_sc0005860.1_g000002
rosa_roxburghii	Rroxscaffold_1G00014260
rosa_rugosa	Rorug05G0379000
rosa_samantha	Rh5AG438700 Rh5BG455600 Rh5CG477700 Rh5DG470400
rosa_wichuraiana	Rw5G041020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	16
AccB1I	GGYRCC	2 cut(s)	16, 100
AciI	CCGC	1 cut(s)	166
AclWI	GGATC	3 cut(s)	46, 443, 470
AcuI	CTGAAG	2 cut(s)	87, 321
AfaI	GTAC	5 cut(s)	18, 195, 267, 359, 1141
AfiI	CCNNNNNNNGG	1 cut(s)	385
AflIII	ACRYGT	1 cut(s)	1030
AgsI	TTSAA	3 cut(s)	159, 230, 1175
AluBI	AGCT	4 cut(s)	179, 415, 770, 1098
AluI	AGCT	4 cut(s)	179, 415, 770, 1098
Alw21I	GWGCWC	1 cut(s)	144
Alw26I	GTCTC	3 cut(s)	42, 67, 433
AlwI	GGATC	3 cut(s)	46, 443, 470
AoxI	GGCC	1 cut(s)	842
ApeKI	GCWGC	3 cut(s)	278, 412, 512
ArsI	GACNNNNNNTTYG	2 cut(s)	152, 184
Asp718I	GGTACC	1 cut(s)	16
AspLEI	GCGC	2 cut(s)	999, 1042
AsuHPI	GGTGA	3 cut(s)	368, 496, 823
BaeGI	GKGCMC	1 cut(s)	400
BanI	GGYRCC	2 cut(s)	16, 100
Bbv12I	GWGCWC	1 cut(s)	144
BbvI	GCAGC	3 cut(s)	290, 424, 524
BccI	CCATC	3 cut(s)	579, 808, 937
BceAI	ACGGC	1 cut(s)	1076
BcoDI	GTCTC	3 cut(s)	42, 67, 433
BfaI	CTAG	2 cut(s)	980, 1101
BfmI	CTRYAG	1 cut(s)	145
BfoI	RGCGCY	1 cut(s)	1043
BisI	GCNGC	3 cut(s)	279, 413, 513
BlsI	GCNGC	3 cut(s)	280, 414, 514
BmcAI	AGTACT	2 cut(s)	267, 1141
BmiI	GGNNCC	2 cut(s)	18, 102
BmsI	GCATC	6 cut(s)	215, 265, 837, 906, 999, 1008
BpmI	CTGGAG	2 cut(s)	714, 843
Bpu10I	CCTNAGC	1 cut(s)	589
BpuEI	CTTGAG	1 cut(s)	404
Bsa29I	ATCGAT	1 cut(s)	914
BsaBI	GATNNNNATC	4 cut(s)	440, 603, 627, 968
BsaI	GGTCTC	1 cut(s)	433
BsaJI	CCNNGG	1 cut(s)	763
Bsc4I	CCNNNNNNNGG	1 cut(s)	385
Bse1I	ACTGG	1 cut(s)	361
Bse3DI	GCAATG	1 cut(s)	312
Bse8I	GATNNNNATC	4 cut(s)	440, 603, 627, 968
BseCI	ATCGAT	1 cut(s)	914
BseDI	CCNNGG	1 cut(s)	763
BseGI	GGATG	1 cut(s)	1021
BseJI	GATNNNNATC	4 cut(s)	440, 603, 627, 968
BseLI	CCNNNNNNNGG	1 cut(s)	385
BseMI	GCAATG	1 cut(s)	312
BseMII	CTCAG	1 cut(s)	53
BseNI	ACTGG	1 cut(s)	361
BseSI	GKGCMC	1 cut(s)	400
BseXI	GCAGC	3 cut(s)	290, 424, 524
BsgI	GTGCAG	2 cut(s)	431, 856
BshFI	GGCC	1 cut(s)	844
BshNI	GGYRCC	2 cut(s)	16, 100
BshVI	ATCGAT	1 cut(s)	914
BsiHKAI	GWGCWC	1 cut(s)	144
BsiSI	CCGG	1 cut(s)	975
BslI	CCNNNNNNNGG	1 cut(s)	385
BsmAI	GTCTC	3 cut(s)	42, 67, 433
BsnI	GGCC	1 cut(s)	844
Bso31I	GGTCTC	1 cut(s)	433
Bsp1286I	GDGCHC	2 cut(s)	144, 400
Bsp143I	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
BspACI	CCGC	1 cut(s)	166
BspANI	GGCC	1 cut(s)	844
BspCNI	CTCAG	1 cut(s)	52
BspDI	ATCGAT	1 cut(s)	914
BspHI	TCATGA	1 cut(s)	661
BspLI	GGNNCC	2 cut(s)	18, 102
BspPI	GGATC	3 cut(s)	46, 443, 470
BspT107I	GGYRCC	2 cut(s)	16, 100
BspTNI	GGTCTC	1 cut(s)	433
BsrDI	GCAATG	1 cut(s)	312
BsrI	ACTGG	1 cut(s)	361
BssECI	CCNNGG	1 cut(s)	763
BssMI	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
BssT1I	CCWWGG	1 cut(s)	763
Bst4CI	ACNGT	6 cut(s)	146, 328, 380, 1048, 1060, 1133
BstC8I	GCNNGC	1 cut(s)	1089
BstDEI	CTNAG	3 cut(s)	39, 474, 589
BstF5I	GGATG	1 cut(s)	1021
BstH2I	RGCGCY	1 cut(s)	1043
BstHHI	GCGC	2 cut(s)	999, 1042
BstKTI	GATC	8 cut(s)	54, 82, 438, 444, 465, 601, 625, 865
BstMAI	GTCTC	3 cut(s)	42, 67, 433
BstMBI	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
BstMWI	GCNNNNNNNGC	2 cut(s)	421, 996
BstNSI	RCATGY	2 cut(s)	582, 782
BstSFI	CTRYAG	1 cut(s)	145
BstSLI	GKGCMC	1 cut(s)	400
BstV1I	GCAGC	3 cut(s)	290, 424, 524
BstX2I	RGATCY	2 cut(s)	51, 435
BstYI	RGATCY	2 cut(s)	51, 435
Bsu15I	ATCGAT	1 cut(s)	914
BsuRI	GGCC	1 cut(s)	844
BsuTUI	ATCGAT	1 cut(s)	914
BtgZI	GCGATG	1 cut(s)	769
BtsCI	GGATG	1 cut(s)	1021
BtsIMutI	CAGTG	2 cut(s)	142, 291
Cac8I	GCNNGC	1 cut(s)	1089
CciI	TCATGA	1 cut(s)	661
CfoI	GCGC	2 cut(s)	999, 1042
ClaI	ATCGAT	1 cut(s)	914
Csp6I	GTAC	5 cut(s)	17, 194, 266, 358, 1140
CviQI	GTAC	5 cut(s)	17, 194, 266, 358, 1140
DdeI	CTNAG	3 cut(s)	39, 474, 589
DpnI	GATC	8 cut(s)	53, 81, 437, 443, 464, 600, 624, 864
DpnII	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
Eco130I	CCWWGG	1 cut(s)	763
Eco31I	GGTCTC	1 cut(s)	433
Eco32I	GATATC	2 cut(s)	374, 970
Eco57I	CTGAAG	2 cut(s)	87, 321
EcoRV	GATATC	2 cut(s)	374, 970
EcoT14I	CCWWGG	1 cut(s)	763
EcoT22I	ATGCAT	1 cut(s)	580
ErhI	CCWWGG	1 cut(s)	763
FalI	AAGNNNNNCTT	1 cut(s)	37
FauNDI	CATATG	2 cut(s)	419, 833
Fnu4HI	GCNGC	3 cut(s)	279, 413, 513
FokI	GGATG	1 cut(s)	1028
Fsp4HI	GCNGC	3 cut(s)	279, 413, 513
FspBI	CTAG	2 cut(s)	980, 1101
GlaI	GCGC	2 cut(s)	998, 1041
GluI	GCNGC	3 cut(s)	279, 413, 513
GsuI	CTGGAG	2 cut(s)	714, 843
HaeII	RGCGCY	1 cut(s)	1043
HaeIII	GGCC	1 cut(s)	844
HapII	CCGG	1 cut(s)	975
HhaI	GCGC	2 cut(s)	999, 1042
Hin6I	GCGC	2 cut(s)	997, 1040
HinP1I	GCGC	2 cut(s)	997, 1040
HincII	GTYRAC	2 cut(s)	457, 532
HindII	GTYRAC	2 cut(s)	457, 532
HinfI	GANTC	5 cut(s)	35, 155, 306, 709, 796
HpaII	CCGG	1 cut(s)	975
HphI	GGTGA	3 cut(s)	368, 496, 823
Hpy166II	GTNNAC	5 cut(s)	457, 469, 487, 532, 1021
Hpy188I	TCNGA	4 cut(s)	67, 371, 1084, 1107
Hpy8I	GTNNAC	5 cut(s)	457, 469, 487, 532, 1021
HpyAV	CCTTC	2 cut(s)	7, 62
HpyCH4III	ACNGT	6 cut(s)	146, 328, 380, 1048, 1060, 1133
HpyCH4IV	ACGT	2 cut(s)	1032, 1079
HpyCH4V	TGCA	8 cut(s)	263, 278, 412, 512, 578, 686, 778, 873
HpyF10VI	GCNNNNNNNGC	2 cut(s)	421, 996
HpyF3I	CTNAG	3 cut(s)	39, 474, 589
HpySE526I	ACGT	2 cut(s)	1032, 1079
HspAI	GCGC	2 cut(s)	997, 1040
KpnI	GGTACC	1 cut(s)	20
Kzo9I	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
LmnI	GCTCC	2 cut(s)	113, 767
Lsp1109I	GCAGC	3 cut(s)	290, 424, 524
LweI	GCATC	6 cut(s)	215, 265, 837, 906, 999, 1008
MaeI	CTAG	2 cut(s)	980, 1101
MaeII	ACGT	2 cut(s)	1032, 1079
MaeIII	GTNAC	5 cut(s)	492, 557, 593, 1075, 1159
MalI	GATC	8 cut(s)	53, 81, 437, 443, 464, 600, 624, 864
MboI	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
MboII	GAAGA	2 cut(s)	74, 689
MflI	RGATCY	2 cut(s)	51, 435
MhlI	GDGCHC	2 cut(s)	144, 400
MluCI	AATT	2 cut(s)	45, 1110
MlyI	GAGTC	2 cut(s)	44, 718
MmeI	TCCRAC	2 cut(s)	283, 1030
MnlI	CCTC	8 cut(s)	114, 340, 379, 442, 578, 667, 688, 818
Mph1103I	ATGCAT	1 cut(s)	580
MseI	TTAA	3 cut(s)	536, 749, 921
MslI	CAYNNNNRTG	7 cut(s)	218, 579, 606, 630, 654, 660, 941
MspA1I	CMGCKG	1 cut(s)	166
MspI	CCGG	1 cut(s)	975
MwoI	GCNNNNNNNGC	2 cut(s)	421, 996
NdeI	CATATG	2 cut(s)	419, 833
NdeII	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
NlaIV	GGNNCC	2 cut(s)	18, 102
NmuCI	GTSAC	2 cut(s)	557, 593
NsiI	ATGCAT	1 cut(s)	580
NspI	RCATGY	2 cut(s)	582, 782
PagI	TCATGA	1 cut(s)	661
PcsI	WCGNNNNNNNCGW	1 cut(s)	1029
PfeI	GAWTC	3 cut(s)	155, 306, 796
PkrI	GCNGC	3 cut(s)	280, 414, 514
PleI	GAGTC	2 cut(s)	43, 717
PpsI	GAGTC	2 cut(s)	43, 717
PspN4I	GGNNCC	2 cut(s)	18, 102
PsuI	RGATCY	2 cut(s)	51, 435
RsaI	GTAC	5 cut(s)	18, 195, 267, 359, 1141
RsaNI	GTAC	5 cut(s)	17, 194, 266, 358, 1140
RseI	CAYNNNNRTG	7 cut(s)	218, 579, 606, 630, 654, 660, 941
SaqAI	TTAA	3 cut(s)	536, 749, 921
SatI	GCNGC	3 cut(s)	279, 413, 513
Sau3AI	GATC	8 cut(s)	51, 79, 435, 441, 462, 598, 622, 862
ScaI	AGTACT	2 cut(s)	267, 1141
SchI	GAGTC	2 cut(s)	44, 718
SduI	GDGCHC	2 cut(s)	144, 400
SfaNI	GCATC	6 cut(s)	215, 265, 837, 906, 999, 1008
SfcI	CTRYAG	1 cut(s)	145
SmiMI	CAYNNNNRTG	7 cut(s)	218, 579, 606, 630, 654, 660, 941
SmlI	CTYRAG	1 cut(s)	383
SmoI	CTYRAG	1 cut(s)	383
Sse9I	AATT	2 cut(s)	45, 1110
SsiI	CCGC	1 cut(s)	166
SspI	AATATT	1 cut(s)	747
SspMI	CTAG	2 cut(s)	980, 1101
StyI	CCWWGG	1 cut(s)	763
TaaI	ACNGT	6 cut(s)	146, 328, 380, 1048, 1060, 1133
TaiI	ACGT	2 cut(s)	1035, 1082
TaqI	TCGA	1 cut(s)	914
TasI	AATT	2 cut(s)	45, 1110
TatI	WGTACW	3 cut(s)	193, 265, 1139
TfiI	GAWTC	3 cut(s)	155, 306, 796
Tru1I	TTAA	3 cut(s)	536, 749, 921
Tru9I	TTAA	3 cut(s)	536, 749, 921
TscAI	CASTG	2 cut(s)	149, 298
TseFI	GTSAC	2 cut(s)	557, 593
TseI	GCWGC	3 cut(s)	278, 412, 512
Tsp45I	GTSAC	2 cut(s)	557, 593
TspDTI	ATGAA	1 cut(s)	602
TspGWI	ACGGA	1 cut(s)	793
TspRI	CASTG	2 cut(s)	149, 298
XceI	RCATGY	2 cut(s)	582, 782
XcmI	CCANNNNNNNNNTGG	1 cut(s)	578
XspI	CTAG	2 cut(s)	980, 1101
ZrmI	AGTACT	2 cut(s)	267, 1141
Zsp2I	ATGCAT	1 cut(s)	580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.