FvH4_3g38110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008787
Physical Location & Seq
Reverse (-)
32558342 .. 32559659
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38110.t1

Sequence Viewer

Length: 489 bp
ATGACTTGGAAGCGCTATCAGCAGGCTCAAGCAGGTTACGAGGCGGCGAAATCGTTGAATGCCCAGAAGTTCTTGAAGAAAATAGGATGCGGCAAGGAGGACTATCACTTCTGGAAGCAAATCGGCAAGGCTTTACTATGCACATACACACTGGGTGGAGCAGCTTGGCTCTACAATGAGAAATCGCCACTCGGATGGTGGACGCTGAAGCCTCTTCCCAAGGAAGAGAGAGAGCTAGCTCACTTGTATGAGCGGCGAGAGTTTCCATACCCGGGAGACGAAGAAGCAATGGAAGAGTTTGTGGCCAAAGGTGGCATGATCGGCACGACGATTGGCCCGAAAGGGTTCATTGAGACGGATAAAGATGCGCTGAACTATCAGAAGGAGCTGCAGAACAAGAAGTTTGAGCAGGAAGCGCAGAAGCTGTGGATCAGGATGAGGAATGAGGTTATTTCGGAGCTTCAAGAGAAGGGCTATGATGTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.94

Weight (kDa)

6.77

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 23
AccBSI CCGCTC 1 cut(s) 253
AciI CCGC 3 cut(s) 44, 90, 253
AclWI GGATC 1 cut(s) 437
AcoI YGGCCR 1 cut(s) 303
AcuI CTGAAG 1 cut(s) 227
AdeI CACNNNGTG 1 cut(s) 155
AfeI AGCGCT 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 3 cut(s) 58, 76, 464
AluBI AGCT 6 cut(s) 164, 235, 239, 388, 424, 460
AluI AGCT 6 cut(s) 164, 235, 239, 388, 424, 460
Alw26I GTCTC 2 cut(s) 270, 347
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 424
Ama87I CYCGRG 1 cut(s) 271
Aor51HI AGCGCT 1 cut(s) 14
AoxI GGCC 2 cut(s) 303, 334
ApeKI GCWGC 2 cut(s) 161, 388
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 3 cut(s) 15, 370, 418
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 2 cut(s) 272, 273
AsuNHI GCTAGC 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 271
BalI TGGCCA 1 cut(s) 305
BbvI GCAGC 2 cut(s) 173, 375
BccI CCATC 1 cut(s) 189
BcnI CCSGG 2 cut(s) 272, 273
BcoDI GTCTC 2 cut(s) 270, 347
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 389
BfoI RGCGCY 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 23
BisI GCNGC 5 cut(s) 45, 91, 162, 254, 389
BlsI GCNGC 5 cut(s) 46, 92, 163, 255, 390
Bme1390I CCNGG 2 cut(s) 272, 273
BmeT110I CYCGRG 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 335
BmrFI CCNGG 2 cut(s) 272, 273
BmrI ACTGGG 1 cut(s) 161
BmsI GCATC 2 cut(s) 77, 355
BmtI GCTAGC 1 cut(s) 239
BmuI ACTGGG 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 12
BpuMI CCSGG 2 cut(s) 272, 273
BsaJI CCNNGG 2 cut(s) 219, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 219, 271
BseGI GGATG 3 cut(s) 92, 200, 441
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMI GCAATG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 156
BseXI GCAGC 2 cut(s) 173, 375
BshFI GGCC 2 cut(s) 305, 336
BsiHKCI CYCGRG 1 cut(s) 271
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 2 cut(s) 270, 347
BsmBI CGTCTC 2 cut(s) 270, 347
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 2 cut(s) 305, 336
BsoBI CYCGRG 1 cut(s) 271
Bsp143I GATC 2 cut(s) 318, 429
BspACI CCGC 3 cut(s) 44, 90, 253
BspANI GGCC 2 cut(s) 305, 336
BspMAI CTGCAG 1 cut(s) 393
BspMI ACCTGC 1 cut(s) 23
BspOI GCTAGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 437
BsrBI CCGCTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 219, 271
BssMI GATC 2 cut(s) 318, 429
BssT1I CCWWGG 1 cut(s) 219
Bst6I CTCTTC 3 cut(s) 219, 219, 288
BstC8I GCNNGC 2 cut(s) 24, 237
BstF5I GGATG 3 cut(s) 92, 200, 441
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 3 cut(s) 15, 370, 418
BstKTI GATC 2 cut(s) 321, 432
BstMAI GTCTC 2 cut(s) 270, 347
BstMBI GATC 2 cut(s) 318, 429
BstMWI GCNNNNNNNGC 3 cut(s) 19, 321, 415
BstSCI CCNGG 2 cut(s) 270, 271
BstSFI CTRYAG 1 cut(s) 389
BstV1I GCAGC 2 cut(s) 173, 375
BstXI CCANNNNNNTGG 1 cut(s) 195
BsuRI GGCC 2 cut(s) 305, 336
BtsCI GGATG 3 cut(s) 92, 200, 441
BtsIMutI CAGTG 1 cut(s) 149
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 2 cut(s) 24, 237
CaiI CAGNNNCTG 1 cut(s) 424
CfoI GCGC 3 cut(s) 15, 370, 418
Cfr13I GGNCC 1 cut(s) 335
Cfr9I CCCGGG 1 cut(s) 271
CseI GACGC 1 cut(s) 211
CviAII CATG 1 cut(s) 316
DpnI GATC 2 cut(s) 320, 431
DpnII GATC 2 cut(s) 318, 429
DraIII CACNNNGTG 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 3 cut(s) 219, 219, 288
EarI CTCTTC 3 cut(s) 219, 219, 288
Eco130I CCWWGG 1 cut(s) 219
Eco47III AGCGCT 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
Esp3I CGTCTC 2 cut(s) 270, 347
FaeI CATG 1 cut(s) 319
FaiI YATR 6 cut(s) 139, 145, 249, 268, 317, 477
FatI CATG 1 cut(s) 315
Fnu4HI GCNGC 5 cut(s) 45, 91, 162, 254, 389
FokI GGATG 3 cut(s) 99, 207, 448
Fsp4HI GCNGC 5 cut(s) 45, 91, 162, 254, 389
FspBI CTAG 1 cut(s) 236
GlaI GCGC 3 cut(s) 14, 369, 417
GluI GCNGC 5 cut(s) 45, 91, 162, 254, 389
HaeII RGCGCY 1 cut(s) 16
HaeIII GGCC 2 cut(s) 305, 336
HapII CCGG 1 cut(s) 272
HgaI GACGC 1 cut(s) 211
HhaI GCGC 3 cut(s) 15, 370, 418
Hin1II CATG 1 cut(s) 319
Hin6I GCGC 3 cut(s) 13, 368, 416
HinP1I GCGC 3 cut(s) 13, 368, 416
HpaII CCGG 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 3 cut(s) 194, 381, 457
Hpy188III TCNNGA 4 cut(s) 73, 112, 433, 464
Hpy8I GTNNAC 1 cut(s) 201
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 376, 463
HpyCH4V TGCA 2 cut(s) 141, 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 321, 415
Hsp92II CATG 1 cut(s) 319
HspAI GCGC 3 cut(s) 13, 368, 416
Kzo9I GATC 2 cut(s) 318, 429
LmnI GCTCC 3 cut(s) 158, 385, 457
LpnPI CCDG 8 cut(s) 8, 18, 77, 97, 137, 285, 395, 418
Lsp1109I GCAGC 2 cut(s) 173, 375
LweI GCATC 2 cut(s) 77, 355
MaeI CTAG 1 cut(s) 236
MaeIII GTNAC 1 cut(s) 35
MalI GATC 2 cut(s) 320, 431
MbiI CCGCTC 1 cut(s) 253
MboI GATC 2 cut(s) 318, 429
MboII GAAGA 5 cut(s) 88, 206, 236, 293, 305
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MnlI CCTC 5 cut(s) 34, 91, 222, 432, 439
Mox20I TGGCCA 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 344
MscI TGGCCA 1 cut(s) 305
MslI CAYNNNNRTG 2 cut(s) 193, 246
Msp20I TGGCCA 1 cut(s) 305
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 2 cut(s) 272, 273
Mva1269I GAATGC 1 cut(s) 64
MwoI GCNNNNNNNGC 3 cut(s) 19, 321, 415
NciI CCSGG 2 cut(s) 272, 273
NdeII GATC 2 cut(s) 318, 429
NheI GCTAGC 1 cut(s) 235
NlaIII CATG 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 344
PkrI GCNGC 5 cut(s) 46, 92, 163, 255, 390
PspPI GGNCC 1 cut(s) 335
PstI CTGCAG 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 424
RseI CAYNNNNRTG 2 cut(s) 193, 246
SatI GCNGC 5 cut(s) 45, 91, 162, 254, 389
Sau3AI GATC 2 cut(s) 318, 429
Sau96I GGNCC 1 cut(s) 335
ScrFI CCNGG 2 cut(s) 272, 273
SetI ASST 9 cut(s) 37, 166, 237, 241, 313, 390, 426, 450, 462
SfaNI GCATC 2 cut(s) 77, 355
SfcI CTRYAG 1 cut(s) 389
SmaI CCCGGG 1 cut(s) 273
SmiMI CAYNNNNRTG 2 cut(s) 193, 246
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
SsiI CCGC 3 cut(s) 44, 90, 253
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 270, 271
StyI CCWWGG 1 cut(s) 219
TauI GCSGC 3 cut(s) 47, 93, 256
TscAI CASTG 1 cut(s) 156
TseI GCWGC 2 cut(s) 161, 388
TspDTI ATGAA 1 cut(s) 337
TspGWI ACGGA 1 cut(s) 371
TspMI CCCGGG 1 cut(s) 271
TspRI CASTG 1 cut(s) 156
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
XmaI CCCGGG 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.