MD03G1077000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008787
Physical Location & Seq
Forward (+)
6269854 .. 6270761
908 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1077000.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGTTCTCAAACGCTATCAGGATGCTAAAGCAGGATACCAATTGGCCAAATCGTTCAATGCCGAGAAGTACTTGAAAAAAGTGGGATTGGGCAAAGAAGACTACTACTTCTGGAAGCAAATTGGCAAGGCATTGGTATGCACATACACTCTGTTCGGCGTGGCCTGGCTCTACAACGAGAAATCGCCACTCGGGTGGTGGACGCTCAAGCCTAGGCCCAAGGAAGAGAGAGAGCTAGCGCACCTGTACGAGCGTCGTGAGTTCCCGTACCCTGGGGATGAAGAAGCCATGGAAGAGTTTGTTGCCAAGGGGGGAATGATCGGCACGACAATTGGCCCGAAAGGGTTTGTCGAGACGGACAAAGATGCGTTTAATTATCAGAAAGAGCTGCAGAAGAAGAAGCTGGAGCAGGAAGCTCAGAAGCTGTGGGTGCGGATGAAGAATGAGGTCGTCTCCGAGCTTCAAGAGAAGGGTTACGTCGTTGACGCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

19.22

Weight (kDa)

8.41

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 433
AcoI YGGCCR 1 cut(s) 45
AfaI GTAC 3 cut(s) 71, 248, 269
AfiI CCNNNNNNNGG 1 cut(s) 272
AgsI TTSAA 3 cut(s) 58, 76, 464
AjnI CCWGG 2 cut(s) 164, 271
AleI CACNNNNGTG 1 cut(s) 193
AluBI AGCT 6 cut(s) 235, 388, 403, 416, 424, 460
AluI AGCT 6 cut(s) 235, 388, 403, 416, 424, 460
Alw26I GTCTC 2 cut(s) 347, 457
AlwNI CAGNNNCTG 1 cut(s) 424
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 4 cut(s) 45, 162, 215, 334
ApeKI GCWGC 1 cut(s) 388
AspA2I CCTAGG 1 cut(s) 212
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 2 cut(s) 216, 335
AsuNHI GCTAGC 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 191
AvrII CCTAGG 1 cut(s) 212
BalI TGGCCA 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 375
BciT130I CCWGG 2 cut(s) 166, 273
BciVI GTATCC 1 cut(s) 29
BcoDI GTCTC 2 cut(s) 347, 457
BfaI CTAG 2 cut(s) 213, 236
BfmI CTRYAG 1 cut(s) 389
BfuI GTATCC 1 cut(s) 29
BisI GCNGC 1 cut(s) 389
BlnI CCTAGG 1 cut(s) 212
BlsI GCNGC 1 cut(s) 390
BmcAI AGTACT 1 cut(s) 71
Bme1390I CCNGG 2 cut(s) 166, 273
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 216, 335
BmrFI CCNGG 2 cut(s) 166, 273
BmsI GCATC 2 cut(s) 13, 355
BmtI GCTAGC 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 191
BsaJI CCNNGG 6 cut(s) 212, 219, 271, 272, 288, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 272
BseBI CCWGG 2 cut(s) 166, 273
BseDI CCNNGG 6 cut(s) 212, 219, 271, 272, 288, 306
BseGI GGATG 3 cut(s) 28, 283, 441
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMII CTCAG 2 cut(s) 431, 480
BseXI GCAGC 1 cut(s) 375
BshFI GGCC 4 cut(s) 47, 164, 217, 336
BsiHKCI CYCGRG 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 2 cut(s) 347, 457
BsmBI CGTCTC 2 cut(s) 347, 457
BsnI GGCC 4 cut(s) 47, 164, 217, 336
BsoBI CYCGRG 1 cut(s) 191
Bsp143I GATC 1 cut(s) 318
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 1 cut(s) 433
BspANI GGCC 4 cut(s) 47, 164, 217, 336
BspCNI CTCAG 2 cut(s) 430, 481
BspMAI CTGCAG 1 cut(s) 393
BspOI GCTAGC 1 cut(s) 239
BssECI CCNNGG 6 cut(s) 212, 219, 271, 272, 288, 306
BssMI GATC 1 cut(s) 318
BssT1I CCWWGG 4 cut(s) 212, 219, 288, 306
Bst2UI CCWGG 2 cut(s) 166, 273
Bst6I CTCTTC 2 cut(s) 219, 288
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 2 cut(s) 417, 489
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 3 cut(s) 28, 283, 441
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 2 cut(s) 347, 457
BstMBI GATC 1 cut(s) 318
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 2 cut(s) 166, 273
BstSCI CCNGG 2 cut(s) 164, 271
BstSFI CTRYAG 1 cut(s) 389
BstV1I GCAGC 1 cut(s) 375
BstV2I GAAGAC 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 195
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 4 cut(s) 47, 164, 217, 336
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 3 cut(s) 28, 283, 441
Cac8I GCNNGC 1 cut(s) 237
CaiI CAGNNNCTG 1 cut(s) 424
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 2 cut(s) 216, 335
CseI GACGC 2 cut(s) 211, 242
Csp6I GTAC 3 cut(s) 70, 247, 268
CviAII CATG 1 cut(s) 289
CviQI GTAC 3 cut(s) 70, 247, 268
DdeI CTNAG 2 cut(s) 417, 489
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 2 cut(s) 219, 288
EarI CTCTTC 2 cut(s) 219, 288
Eco130I CCWWGG 4 cut(s) 212, 219, 288, 306
Eco88I CYCGRG 1 cut(s) 191
EcoRII CCWGG 2 cut(s) 164, 271
EcoT14I CCWWGG 4 cut(s) 212, 219, 288, 306
ErhI CCWWGG 4 cut(s) 212, 219, 288, 306
Esp3I CGTCTC 2 cut(s) 347, 457
FaeI CATG 1 cut(s) 292
FaiI YATR 3 cut(s) 139, 145, 290
FatI CATG 1 cut(s) 288
Fnu4HI GCNGC 1 cut(s) 389
FokI GGATG 3 cut(s) 35, 290, 448
Fsp4HI GCNGC 1 cut(s) 389
FspBI CTAG 2 cut(s) 213, 236
GlaI GCGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 425
HaeIII GGCC 4 cut(s) 47, 164, 217, 336
HgaI GACGC 2 cut(s) 211, 242
HhaI GCGC 1 cut(s) 241
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
Hpy166II GTNNAC 2 cut(s) 201, 484
Hpy188I TCNGA 3 cut(s) 381, 420, 457
Hpy188III TCNNGA 5 cut(s) 20, 112, 257, 352, 464
Hpy8I GTNNAC 2 cut(s) 201, 484
Hpy99I CGWCG 2 cut(s) 258, 482
HpyAV CCTTC 1 cut(s) 463
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 2 cut(s) 141, 391
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 2 cut(s) 417, 489
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 1 cut(s) 375
LweI GCATC 2 cut(s) 13, 355
MaeI CTAG 2 cut(s) 213, 236
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 1 cut(s) 473
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 7 cut(s) 110, 236, 293, 305, 406, 409, 451
MfeI CAATTG 2 cut(s) 41, 330
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 4 cut(s) 41, 120, 330, 373
MluNI TGGCCA 1 cut(s) 47
MnlI CCTC 1 cut(s) 439
Mox20I TGGCCA 1 cut(s) 47
MscI TGGCCA 1 cut(s) 47
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 2 cut(s) 136, 193
Msp20I TGGCCA 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 166, 273
MunI CAATTG 2 cut(s) 41, 330
MvaI CCWGG 2 cut(s) 166, 273
MwoI GCNNNNNNNGC 1 cut(s) 430
NcoI CCATGG 1 cut(s) 288
NdeII GATC 1 cut(s) 318
NheI GCTAGC 1 cut(s) 235
NlaIII CATG 1 cut(s) 292
NmeAIII GCCGAG 1 cut(s) 88
OliI CACNNNNGTG 1 cut(s) 193
PasI CCCWGGG 1 cut(s) 272
PkrI GCNGC 1 cut(s) 390
Psp6I CCWGG 2 cut(s) 164, 271
PspGI CCWGG 2 cut(s) 164, 271
PspPI GGNCC 2 cut(s) 216, 335
PstI CTGCAG 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 424
RsaI GTAC 3 cut(s) 71, 248, 269
RsaNI GTAC 3 cut(s) 70, 247, 268
RseI CAYNNNNRTG 2 cut(s) 136, 193
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 1 cut(s) 389
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 2 cut(s) 216, 335
ScaI AGTACT 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 166, 273
SetI ASST 9 cut(s) 237, 246, 390, 405, 418, 426, 450, 462, 480
SfaNI GCATC 2 cut(s) 13, 355
SfcI CTRYAG 1 cut(s) 389
SmiMI CAYNNNNRTG 2 cut(s) 136, 193
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 4 cut(s) 41, 120, 330, 373
SsiI CCGC 1 cut(s) 433
SspMI CTAG 2 cut(s) 213, 236
StyD4I CCNGG 2 cut(s) 164, 271
StyI CCWWGG 4 cut(s) 212, 219, 288, 306
TaiI ACGT 1 cut(s) 480
TaqI TCGA 1 cut(s) 351
TasI AATT 4 cut(s) 41, 120, 330, 373
TatI WGTACW 1 cut(s) 69
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseI GCWGC 1 cut(s) 388
TspDTI ATGAA 2 cut(s) 294, 452
TspGWI ACGGA 1 cut(s) 371
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
XmaJI CCTAGG 1 cut(s) 212
XspI CTAG 2 cut(s) 213, 236
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.