FvH4_3g39830

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014704
Physical Location & Seq
Reverse (-)
33748774 .. 33752033
3260 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39830.t2

Sequence Viewer

Length: 2415 bp
ATGAATAGAACACACAACTGGATATGCGAGACCCAGAAAAGCATGAGATCGAAAGGGGTCTTTCTTGGTGATAATCCTTTGAAATACCCAACTCCGATACTTTATATGCAGCTTTCTATAGCTGCCTTGGTCACCATGCTCTTCCAATCCGTTCTCAACCCTCTTGGAGAAAGTGCTTTCATCTCGCAAATGCTGGCAGGTATTCTATTGGGACCATCATGCTTAGGAGGAGAAAATGATTTTGCAGACACAGTTTACCCAGTAAAAAGTTTCTACATCAGCCAAACCTTTGCTTTCTTTGGTTGCATGCTTTTCCTATTCCTAGTGGGTGTAAAAATGGATATGAGTATAGTCAAACGGTCGGGAAAGAAGGCTGTGGTCATAGGCCTCTCGGCATTTTTCGTCCCTTTGGTCCTAAATGTATGCTTAACTTTTGTACTGCAACGAACGGTTACCATGGAACCCCAGCTGCACAAATCACTTATAGTCATAGCAGTGCTTCAGTGCCAAAGTAGTTTCCACGTCATTGCTTGTCTCTTGGGAGATTTGAAGCTCCTAAACTCGGAGCTTGGTCGTTTGGCTGTGTCTTCTTCGATGATTAGTGGAACCCTCTCTTGGATACATATCATCATATCCTTCACACTCCGGCAAGGCACCATGAAAAATATCCAAAACTTGCCGTTTATGGTTTTGAGTTTAGCCGGATTGTTGGTTCTAATTCTCTGCGTATTGAGGCCAATACTGCTGTGGATGGTTGCACAAGCAGCTAAACGCAAAGCCATCAAAGAGAGCTACATATTCTCCATCTTCATCATGGTCATGCTTTGTTGCCTTCTCGGTGAGATTGTGGGCCAGCATTTTATGCTTGGGCCTATGGTTTTGGGCCTCGCCGTGCCGGACGGCCCGCCTTTGGGTTCAGCTTTGGTGGATAGACTTGACTCGTACGTTTCCAACATTCTGTTGCCGAGCTACTTTGTGTTAACTGGTGCAAGGTTGAATCTGAGTAGAATCAGAATGAAGACCTTTGTGATTGTGGAGGTTCTGGCTCTCAGTAGCTTTTGTGGTAAGCTATTAGGAACCATGGTGCCTTCACTCTACTGCAAAATGCCCGCAACCGACGCACTTGCATTAGGGCTCATCATGAGTGCCCAAGGCATCACTGACCTTCTTATCTTGCAACATGGACTGGTACTCTTGCTCATTGACGGAGAATGCTACAGCATCATGATTATAACCTCGGTTTTATTAACTGGGATCATCACCCCAATAGTGAAATTCTTGTACAAACCCTCCAAGAGATACAAATCCGCAAAGCGAAGACGAACCATTGAGCATTCCTTGCCAAACATGGAACTCAGACTTCTTGTCTGCATGTTCCACCAAGACAGCACTCCCTCCATCATCAACCTAATAGAAGTCTTCAATCCAACCCCAAAAAGCCCCATTTGCTTCTACCTTGTCCACCTCATCCGTCTCGCAGGTCGAACAGCTCCGGTCCTCATAGCCCACAGACCCGGCAAAAGTAACAGCACGACCGCTTTGTCCGATCACATAATCAATGCCTTCAGATTGTACCAAGAACACAACGCCGGCAGAGTCCTAATGAATGCATTCACCGCAATTGCGCCTTTCGACACAATGCACGACGACGTATGCTCACTTGCTTTCGAAAGAAGGACTTCGATGATCATAATCCCTTTTCACAAGTTGTTCTGTGCTCTCCGATGCACCGAGGAAGTAGCACATCCAATCAGGTCCGTCAACAGAAACATCATTCGAAATGCTCCATGCTCCGTTGGGATTCTTGTAGACCGAGGAACGTTGAATAAGAAACCAACAGCTTTACAAGCTTCTCTATCAGGAAGAAGTTTCTATAACGTTGGGACAATCTTTATAGAGGGACCTGATGACCGCGAAGCACTGGCATTAGCGACGAGGATGGCTGGAAACCCTAATGTCAGCCTAACCGTAACCAAATTAACCGATCCGAAGCAAAGCAGCACCCAGAAAGAGATCGATTCGGAAGTTATAAGGAAGTATCAGGAAGCCATTGTTGACAAGAAACAGCATGTTTACAACGAGGTACATGTTAAAGATAGCGTTGACATGATCAATGTGATTAGATCGATGGAGAATTTTCTCGACCTGATTCTGTTGGGGAGAAGGCATGATTGTGATTCGCAGTTGTTCATGGGACTCACTGAATGGAATGAGTTTCCGGAACTAGGGTTCCTCGGAGACATGTTAGCCTCTTCCGACGGAAACTGCGAGGTCTCAGTGCTGGTGGTACAGCAACAATCGTTTGTACCTTCTCAAAAAGAGAAGACTGATGATTTCTTTAGCTGCCATGTCAAAGATTCGTACTCTGTGTTGGATATACCTCATGACAGTACTAACAAAGTGTGGCCACTTTAA

Protein Analysis

805

Amino Acids

89.33

Weight (kDa)

8.78

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 2 - 322 7.2e-26 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 381 - 505 2.5e-15 Plant cation/H(+) antiporter domain
CHX17_C PF23259 525 - 664 1.7e-10 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014704)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39830 FvH4_3g39830
malus_domestica MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica Prupe.6G047700_v2.0.a1
pyrus_communis pycom11g04890
rosa_chinensis RchiOBHm_Chr5g0071771
rosa_laevigata RLG00000036244
rosa_multiflora Rmu_sc0001113.1_g000001
rosa_roxburghii Rroxscaffold_1G00009450
rosa_rugosa Rorug05G0414400 Rorug05G0414500
rosa_samantha Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana Rw5G043720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1232, 2030
AasI GACNNNNNNGTC 1 cut(s) 1540
Acc36I ACCTGC 2 cut(s) 188, 1469
AccB1I GGYRCC 2 cut(s) 653, 1084
AccI GTMKAC 1 cut(s) 1809
AccII CGCG 1 cut(s) 1914
AccIII TCCGGA 1 cut(s) 2218
AciI CCGC 6 cut(s) 905, 1110, 1308, 1536, 1617, 1912
AclI AACGTT 2 cut(s) 1820, 1878
AclWI GGATC 2 cut(s) 1262, 1979
AcoI YGGCCR 1 cut(s) 2405
AcsI RAATTY 2 cut(s) 1274, 2134
AcuI CTGAAG 2 cut(s) 485, 1549
AfiI CCNNNNNNNGG 5 cut(s) 562, 615, 910, 911, 2225
AflIII ACRYGT 2 cut(s) 2086, 2241
AgsI TTSAA 5 cut(s) 82, 550, 997, 1423, 1825
AhdI GACNNNNNGTC 1 cut(s) 1364
AjiI CACGTC 1 cut(s) 523
AjuI GAANNNNNNNTTGG 2 cut(s) 598, 630
AloI GAACNNNNNNTCC 6 cut(s) 696, 728, 2213, 2214, 2245, 2246
Alw21I GWGCWC 1 cut(s) 1720
Alw26I GTCTC 5 cut(s) 23, 539, 1478, 2232, 2278
AlwI GGATC 2 cut(s) 1262, 1979
Aor13HI TCCGGA 1 cut(s) 2218
AoxI GGCC 7 cut(s) 385, 734, 850, 869, 883, 901, 2405
ApeKI GCWGC 6 cut(s) 109, 122, 469, 764, 1998, 2343
ApoI RAATTY 2 cut(s) 1274, 2134
Asp700I GAANNNNTTC 2 cut(s) 1610, 1678
AspLEI GCGC 1 cut(s) 1627
AspS9I GGNCC 9 cut(s) 212, 412, 850, 869, 883, 902, 1495, 1755, 1901
AsuC2I CCSGG 1 cut(s) 1515
AsuHPI GGTGA 5 cut(s) 80, 124, 851, 1252, 1606
AsuII TTCGAA 2 cut(s) 1668, 1777
AvaII GGWCC 5 cut(s) 212, 412, 1495, 1755, 1901
BaeGI GKGCMC 1 cut(s) 1150
BalI TGGCCA 1 cut(s) 2407
BanI GGYRCC 2 cut(s) 653, 1084
BanII GRGCYC 1 cut(s) 1137
BbsI GAAGAC 5 cut(s) 579, 1025, 1324, 1411, 2330
Bbv12I GWGCWC 1 cut(s) 1720
BbvI GCAGC 6 cut(s) 109, 121, 456, 776, 2010, 2330
BccI CCATC 7 cut(s) 223, 745, 788, 812, 1406, 1933, 2122
BceAI ACGGC 3 cut(s) 664, 875, 916
BcgI CGANNNNNNTGC 2 cut(s) 1106, 1140
BciVI GTATCC 1 cut(s) 612
BclI TGATCA 2 cut(s) 1686, 2109
BcnI CCSGG 1 cut(s) 1515
BcoDI GTCTC 5 cut(s) 23, 539, 1478, 2232, 2278
BfaI CTAG 2 cut(s) 323, 2225
BfmI CTRYAG 2 cut(s) 117, 1216
BfuAI ACCTGC 2 cut(s) 188, 1469
BfuI GTATCC 1 cut(s) 612
BisI GCNGC 6 cut(s) 110, 123, 470, 765, 1999, 2344
BlsI GCNGC 6 cut(s) 111, 124, 471, 766, 2000, 2345
BmcAI AGTACT 1 cut(s) 2392
Bme1390I CCNGG 1 cut(s) 1515
Bme18I GGWCC 5 cut(s) 212, 412, 1495, 1755, 1901
BmeRI GACNNNNNGTC 1 cut(s) 1364
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 9 cut(s) 212, 412, 850, 869, 883, 902, 1495, 1755, 1901
BmiI GGNNCC 8 cut(s) 213, 462, 607, 655, 1078, 1086, 1902, 2231
BmrFI CCNGG 1 cut(s) 1515
BmrI ACTGGG 2 cut(s) 254, 1260
BmsI GCATC 3 cut(s) 1164, 1230, 1715
BmuI ACTGGG 2 cut(s) 254, 1260
BpiI GAAGAC 5 cut(s) 579, 1025, 1324, 1411, 2330
Bpu10I CCTNAGC 1 cut(s) 223
Bpu14I TTCGAA 2 cut(s) 1668, 1777
BpuMI CCSGG 1 cut(s) 1515
Bsa29I ATCGAT 2 cut(s) 2016, 2126
BsaBI GATNNNNATC 3 cut(s) 623, 1303, 1691
BsaI GGTCTC 2 cut(s) 23, 2278
BsaJI CCNNGG 8 cut(s) 126, 456, 1080, 1150, 1236, 1731, 1813, 2233
BsaWI WCCGGW 2 cut(s) 1492, 2218
BsaXI ACNNNNNCTCC 4 cut(s) 785, 815, 1274, 1304
Bsc4I CCNNNNNNNGG 5 cut(s) 562, 615, 910, 911, 2225
Bse118I RCCGGY 1 cut(s) 1589
Bse1I ACTGG 6 cut(s) 23, 260, 988, 1191, 1255, 1926
Bse3DI GCAATG 1 cut(s) 525
Bse8I GATNNNNATC 3 cut(s) 623, 1303, 1691
BseAI TCCGGA 1 cut(s) 2218
BseCI ATCGAT 2 cut(s) 2016, 2126
BseDI CCNNGG 8 cut(s) 126, 456, 1080, 1150, 1236, 1731, 1813, 2233
BseGI GGATG 4 cut(s) 756, 1467, 1744, 1944
BseJI GATNNNNATC 3 cut(s) 623, 1303, 1691
BseLI CCNNNNNNNGG 5 cut(s) 562, 615, 910, 911, 2225
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 4 cut(s) 992, 1063, 1369, 2289
BseNI ACTGG 6 cut(s) 23, 260, 988, 1191, 1255, 1926
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 1150
BseXI GCAGC 6 cut(s) 109, 121, 456, 776, 2010, 2330
BseYI CCCAGC 1 cut(s) 465
BsgI GTGCAG 1 cut(s) 455
Bsh1236I CGCG 1 cut(s) 1914
Bsh1285I CGRYCG 2 cut(s) 362, 1536
BshFI GGCC 7 cut(s) 387, 736, 852, 871, 885, 903, 2407
BshNI GGYRCC 2 cut(s) 653, 1084
BshVI ATCGAT 2 cut(s) 2016, 2126
BsiEI CGRYCG 2 cut(s) 362, 1536
BsiHKAI GWGCWC 1 cut(s) 1720
BsiSI CCGG 7 cut(s) 646, 702, 896, 1493, 1515, 1590, 2219
BsiWI CGTACG 1 cut(s) 942
BslFI GGGAC 5 cut(s) 225, 389, 1897, 1914, 2208
BslI CCNNNNNNNGG 5 cut(s) 562, 615, 910, 911, 2225
BsmAI GTCTC 5 cut(s) 23, 539, 1478, 2232, 2278
BsmBI CGTCTC 1 cut(s) 1478
BsmFI GGGAC 5 cut(s) 225, 389, 1897, 1914, 2208
BsmI GAATGC 4 cut(s) 1217, 1333, 1610, 1612
BsnI GGCC 7 cut(s) 387, 736, 852, 871, 885, 903, 2407
Bso31I GGTCTC 2 cut(s) 23, 2278
Bsp119I TTCGAA 2 cut(s) 1668, 1777
Bsp1286I GDGCHC 3 cut(s) 1137, 1150, 1720
Bsp13I TCCGGA 1 cut(s) 2218
Bsp1407I TGTACA 1 cut(s) 1281
Bsp143I GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
Bsp19I CCATGG 2 cut(s) 456, 1080
BspACI CCGC 6 cut(s) 905, 1110, 1308, 1536, 1617, 1912
BspANI GGCC 7 cut(s) 387, 736, 852, 871, 885, 903, 2407
BspCNI CTCAG 4 cut(s) 993, 1062, 1368, 2288
BspDI ATCGAT 2 cut(s) 2016, 2126
BspEI TCCGGA 1 cut(s) 2218
BspFNI CGCG 1 cut(s) 1914
BspHI TCATGA 3 cut(s) 1140, 1224, 2383
BspLI GGNNCC 8 cut(s) 213, 462, 607, 655, 1078, 1086, 1902, 2231
BspMI ACCTGC 2 cut(s) 188, 1469
BspPI GGATC 2 cut(s) 1262, 1979
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 2 cut(s) 1668, 1777
BspT107I GGYRCC 2 cut(s) 653, 1084
BspTNI GGTCTC 2 cut(s) 23, 2278
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 1589
BsrGI TGTACA 1 cut(s) 1281
BsrI ACTGG 6 cut(s) 23, 260, 988, 1191, 1255, 1926
BssAI RCCGGY 1 cut(s) 1589
BssECI CCNNGG 8 cut(s) 126, 456, 1080, 1150, 1236, 1731, 1813, 2233
BssMI GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
BssT1I CCWWGG 4 cut(s) 126, 456, 1080, 1150
Bst4CI ACNGT 5 cut(s) 253, 360, 451, 1969, 2390
Bst6I CTCTTC 2 cut(s) 146, 2257
BstAPI GCANNNNNTGC 2 cut(s) 862, 1339
BstAUI TGTACA 1 cut(s) 1281
BstBI TTCGAA 2 cut(s) 1668, 1777
BstC8I GCNNGC 6 cut(s) 195, 308, 854, 905, 1110, 1591
BstDEI CTNAG 5 cut(s) 223, 1001, 1049, 1355, 2275
BstDSI CCRYGG 2 cut(s) 456, 1080
BstEII GGTNACC 2 cut(s) 130, 451
BstF5I GGATG 4 cut(s) 756, 1467, 1744, 1944
BstFNI CGCG 1 cut(s) 1914
BstHHI GCGC 1 cut(s) 1627
BstKTI GATC 8 cut(s) 50, 1257, 1549, 1689, 1987, 2016, 2112, 2126
BstMAI GTCTC 5 cut(s) 23, 539, 1478, 2232, 2278
BstMBI GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
BstMCI CGRYCG 2 cut(s) 362, 1536
BstMWI GCNNNNNNNGC 8 cut(s) 742, 764, 862, 1118, 1339, 1446, 1616, 1847
BstNSI RCATGY 5 cut(s) 310, 1375, 2072, 2090, 2245
BstPI GGTNACC 2 cut(s) 130, 451
BstSCI CCNGG 1 cut(s) 1513
BstSFI CTRYAG 2 cut(s) 117, 1216
BstSLI GKGCMC 1 cut(s) 1150
BstUI CGCG 1 cut(s) 1914
BstV1I GCAGC 6 cut(s) 109, 121, 456, 776, 2010, 2330
BstV2I GAAGAC 5 cut(s) 579, 1025, 1324, 1411, 2330
Bsu15I ATCGAT 2 cut(s) 2016, 2126
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 7 cut(s) 387, 736, 852, 871, 885, 903, 2407
BsuTUI ATCGAT 2 cut(s) 2016, 2126
BtgI CCRYGG 2 cut(s) 456, 1080
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 4 cut(s) 756, 1467, 1744, 1944
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 6 cut(s) 501, 509, 1158, 1919, 2199, 2283
BveI ACCTGC 2 cut(s) 188, 1469
Cac8I GCNNGC 6 cut(s) 195, 308, 854, 905, 1110, 1591
CciI TCATGA 3 cut(s) 1140, 1224, 2383
CfoI GCGC 1 cut(s) 1627
Cfr10I RCCGGY 1 cut(s) 1589
Cfr13I GGNCC 9 cut(s) 212, 412, 850, 869, 883, 902, 1495, 1755, 1901
ClaI ATCGAT 2 cut(s) 2016, 2126
CseI GACGC 1 cut(s) 1127
DdeI CTNAG 5 cut(s) 223, 1001, 1049, 1355, 2275
DpnI GATC 8 cut(s) 49, 1256, 1548, 1688, 1986, 2015, 2111, 2125
DpnII GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
DrdI GACNNNNNNGTC 1 cut(s) 1540
DriI GACNNNNNGTC 1 cut(s) 1364
DseDI GACNNNNNNGTC 1 cut(s) 1540
EaeI YGGCCR 1 cut(s) 2405
Eam1104I CTCTTC 2 cut(s) 146, 2257
Eam1105I GACNNNNNGTC 1 cut(s) 1364
EarI CTCTTC 2 cut(s) 146, 2257
Eco130I CCWWGG 4 cut(s) 126, 456, 1080, 1150
Eco147I AGGCCT 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 1137
Eco31I GGTCTC 2 cut(s) 23, 2278
Eco47I GGWCC 5 cut(s) 212, 412, 1495, 1755, 1901
Eco57I CTGAAG 2 cut(s) 485, 1549
Eco91I GGTNACC 2 cut(s) 130, 451
EcoO109I RGGNCCY 1 cut(s) 1901
EcoO65I GGTNACC 2 cut(s) 130, 451
EcoT14I CCWWGG 4 cut(s) 126, 456, 1080, 1150
EcoT22I ATGCAT 1 cut(s) 1612
EcoT38I GRGCYC 1 cut(s) 1137
ErhI CCWWGG 4 cut(s) 126, 456, 1080, 1150
Esp3I CGTCTC 1 cut(s) 1478
FalI AAGNNNNNCTT 2 cut(s) 1663, 1695
FaqI GGGAC 5 cut(s) 225, 389, 1897, 1914, 2208
FauI CCCGC 2 cut(s) 912, 1117
FbaI TGATCA 2 cut(s) 1686, 2109
FblI GTMKAC 1 cut(s) 1809
Fnu4HI GCNGC 6 cut(s) 110, 123, 470, 765, 1999, 2344
FokI GGATG 4 cut(s) 763, 1454, 1731, 1951
FriOI GRGCYC 1 cut(s) 1137
Fsp4HI GCNGC 6 cut(s) 110, 123, 470, 765, 1999, 2344
FspBI CTAG 2 cut(s) 323, 2225
GlaI GCGC 1 cut(s) 1626
GluI GCNGC 6 cut(s) 110, 123, 470, 765, 1999, 2344
GsaI CCCAGC 1 cut(s) 469
HaeIII GGCC 7 cut(s) 387, 736, 852, 871, 885, 903, 2407
HapII CCGG 7 cut(s) 646, 702, 896, 1493, 1515, 1590, 2219
HgaI GACGC 1 cut(s) 1127
HhaI GCGC 1 cut(s) 1627
Hin6I GCGC 1 cut(s) 1625
HinP1I GCGC 1 cut(s) 1625
HincII GTYRAC 4 cut(s) 981, 1762, 2056, 2104
HindII GTYRAC 4 cut(s) 981, 1762, 2056, 2104
HindIII AAGCTT 1 cut(s) 1848
HpaI GTTAAC 1 cut(s) 981
HpaII CCGG 7 cut(s) 646, 702, 896, 1493, 1515, 1590, 2219
HphI GGTGA 5 cut(s) 80, 124, 851, 1252, 1606
Hpy166II GTNNAC 8 cut(s) 256, 981, 1462, 1762, 1810, 2056, 2074, 2104
Hpy188III TCNNGA 8 cut(s) 363, 1141, 1225, 1860, 2042, 2141, 2219, 2384
Hpy8I GTNNAC 8 cut(s) 256, 981, 1462, 1762, 1810, 2056, 2074, 2104
Hpy99I CGWCG 5 cut(s) 1121, 1649, 1652, 1936, 2261
HpyAV CCTTC 9 cut(s) 364, 646, 842, 1098, 1175, 1573, 1668, 2157, 2319
HpyCH4III ACNGT 5 cut(s) 253, 360, 451, 1969, 2390
HpyCH4IV ACGT 5 cut(s) 522, 945, 1650, 1820, 1878
HpyF10VI GCNNNNNNNGC 8 cut(s) 742, 764, 862, 1118, 1339, 1446, 1616, 1847
HpyF3I CTNAG 5 cut(s) 223, 1001, 1049, 1355, 2275
HpySE526I ACGT 5 cut(s) 522, 945, 1650, 1820, 1878
HspAI GCGC 1 cut(s) 1625
Kpn2I TCCGGA 1 cut(s) 2218
KroI GCCGGC 1 cut(s) 1589
KroNI GCCGGC 1 cut(s) 1591
Ksp22I TGATCA 2 cut(s) 1686, 2109
KspAI GTTAAC 1 cut(s) 981
Kzo9I GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 5 cut(s) 558, 565, 1495, 1789, 1796
Lsp1109I GCAGC 6 cut(s) 109, 121, 456, 776, 2010, 2330
LweI GCATC 3 cut(s) 1164, 1230, 1715
MaeI CTAG 2 cut(s) 323, 2225
MaeII ACGT 5 cut(s) 522, 945, 1650, 1820, 1878
MaeIII GTNAC 4 cut(s) 130, 451, 1523, 1969
MalI GATC 8 cut(s) 49, 1256, 1548, 1688, 1986, 2015, 2111, 2125
MboI GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
MfeI CAATTG 1 cut(s) 1620
MhlI GDGCHC 3 cut(s) 1137, 1150, 1720
MlsI TGGCCA 1 cut(s) 2407
MluCI AATT 5 cut(s) 717, 1274, 1620, 1976, 2134
MluNI TGGCCA 1 cut(s) 2407
MlyI GAGTC 3 cut(s) 932, 1605, 2190
MmeI TCCRAC 4 cut(s) 975, 1451, 2280, 2352
Mox20I TGGCCA 1 cut(s) 2407
Mph1103I ATGCAT 1 cut(s) 1612
MroI TCCGGA 1 cut(s) 2218
MroNI GCCGGC 1 cut(s) 1589
MroXI GAANNNNTTC 2 cut(s) 1610, 1678
MscI TGGCCA 1 cut(s) 2407
MseI TTAA 6 cut(s) 428, 980, 1247, 1979, 2091, 2413
MslI CAYNNNNRTG 3 cut(s) 494, 818, 2172
Msp20I TGGCCA 1 cut(s) 2407
MspA1I CMGCKG 1 cut(s) 469
MspI CCGG 7 cut(s) 646, 702, 896, 1493, 1515, 1590, 2219
MspR9I CCNGG 1 cut(s) 1515
MunI CAATTG 1 cut(s) 1620
Mva1269I GAATGC 4 cut(s) 1217, 1333, 1610, 1612
MvnI CGCG 1 cut(s) 1914
MwoI GCNNNNNNNGC 8 cut(s) 742, 764, 862, 1118, 1339, 1446, 1616, 1847
NaeI GCCGGC 1 cut(s) 1591
NciI CCSGG 1 cut(s) 1515
NcoI CCATGG 2 cut(s) 456, 1080
NdeII GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
NgoMIV GCCGGC 1 cut(s) 1589
NlaIV GGNNCC 8 cut(s) 213, 462, 607, 655, 1078, 1086, 1902, 2231
NmeAIII GCCGAG 2 cut(s) 371, 990
NmuCI GTSAC 1 cut(s) 130
NsiI ATGCAT 1 cut(s) 1612
NspI RCATGY 5 cut(s) 310, 1375, 2072, 2090, 2245
NspV TTCGAA 2 cut(s) 1668, 1777
PaeI GCATGC 1 cut(s) 310
PagI TCATGA 3 cut(s) 1140, 1224, 2383
PceI AGGCCT 1 cut(s) 387
PciI ACATGT 2 cut(s) 2086, 2241
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 2265
PctI GAATGC 4 cut(s) 1217, 1333, 1610, 1612
PdiI GCCGGC 1 cut(s) 1591
PdmI GAANNNNTTC 2 cut(s) 1610, 1678
PfeI GAWTC 7 cut(s) 997, 1008, 1801, 2018, 2149, 2177, 2357
Pfl23II CGTACG 1 cut(s) 942
PkrI GCNGC 6 cut(s) 111, 124, 471, 766, 2000, 2345
PleI GAGTC 3 cut(s) 932, 1604, 2190
PpsI GAGTC 3 cut(s) 932, 1604, 2190
PpuMI RGGWCCY 1 cut(s) 1901
PscI ACATGT 2 cut(s) 2086, 2241
PsiI TTATAA 2 cut(s) 1232, 2030
Psp1406I AACGTT 2 cut(s) 1820, 1878
Psp5II RGGWCCY 1 cut(s) 1901
PspEI GGTNACC 2 cut(s) 130, 451
PspFI CCCAGC 1 cut(s) 465
PspLI CGTACG 1 cut(s) 942
PspN4I GGNNCC 8 cut(s) 213, 462, 607, 655, 1078, 1086, 1902, 2231
PspPI GGNCC 9 cut(s) 212, 412, 850, 869, 883, 902, 1495, 1755, 1901
PspPPI RGGWCCY 1 cut(s) 1901
PvuII CAGCTG 1 cut(s) 469
RseI CAYNNNNRTG 3 cut(s) 494, 818, 2172
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 6 cut(s) 428, 980, 1247, 1979, 2091, 2413
SatI GCNGC 6 cut(s) 110, 123, 470, 765, 1999, 2344
Sau3AI GATC 8 cut(s) 47, 1254, 1546, 1686, 1984, 2013, 2109, 2123
Sau96I GGNCC 9 cut(s) 212, 412, 850, 869, 883, 902, 1495, 1755, 1901
ScaI AGTACT 1 cut(s) 2392
SchI GAGTC 3 cut(s) 932, 1605, 2190
ScrFI CCNGG 1 cut(s) 1515
SduI GDGCHC 3 cut(s) 1137, 1150, 1720
SfaNI GCATC 3 cut(s) 1164, 1230, 1715
SfcI CTRYAG 2 cut(s) 117, 1216
SfuI TTCGAA 2 cut(s) 1668, 1777
SinI GGWCC 5 cut(s) 212, 412, 1495, 1755, 1901
SmiMI CAYNNNNRTG 3 cut(s) 494, 818, 2172
SphI GCATGC 1 cut(s) 310
Sse9I AATT 5 cut(s) 717, 1274, 1620, 1976, 2134
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 6 cut(s) 905, 1110, 1308, 1536, 1617, 1912
SspMI CTAG 2 cut(s) 323, 2225
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 1513
StyI CCWWGG 4 cut(s) 126, 456, 1080, 1150
TaaI ACNGT 5 cut(s) 253, 360, 451, 1969, 2390
TaiI ACGT 5 cut(s) 525, 948, 1653, 1823, 1881
TaqII GACCGA 1 cut(s) 1827
TasI AATT 5 cut(s) 717, 1274, 1620, 1976, 2134
TatI WGTACW 3 cut(s) 436, 1281, 2390
TfiI GAWTC 7 cut(s) 997, 1008, 1801, 2018, 2149, 2177, 2357
Tru1I TTAA 6 cut(s) 428, 980, 1247, 1979, 2091, 2413
Tru9I TTAA 6 cut(s) 428, 980, 1247, 1979, 2091, 2413
TscAI CASTG 6 cut(s) 501, 509, 1165, 1926, 2206, 2283
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 6 cut(s) 109, 122, 469, 764, 1998, 2343
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 7 cut(s) 17, 169, 674, 799, 1031, 1619, 2179
TspGWI ACGGA 6 cut(s) 139, 1221, 1460, 1747, 1783, 2274
TspRI CASTG 6 cut(s) 501, 509, 1165, 1926, 2206, 2283
VpaK11BI GGWCC 5 cut(s) 212, 412, 1495, 1755, 1901
XapI RAATTY 2 cut(s) 1274, 2134
XceI RCATGY 5 cut(s) 310, 1375, 2072, 2090, 2245
XcmI CCANNNNNNNNNTGG 2 cut(s) 744, 811
XmiI GTMKAC 1 cut(s) 1809
XmnI GAANNNNTTC 2 cut(s) 1610, 1678
XspI CTAG 2 cut(s) 323, 2225
ZrmI AGTACT 1 cut(s) 2392
Zsp2I ATGCAT 1 cut(s) 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.