RLG00000036244

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014704
Physical Location & Seq
Reverse (-)
77993836 .. 77997053
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036244

Sequence Viewer

Length: 2397 bp
ATGAATAGGGCAAACTGGATATGCGAGACCCAGAAAGGCATAAGATCGAAAGGTATCTTTTTGGGTGATAATCCTTTGAAATACCCATCTCCAATACTTCTTCTGCAACTTACTACAGCTGCCTTGGTCACCACTCTCTTCCAATTCATTCTCAACCCTCTCGGAGAAAGTGCTTATATCTCACAAATGCTGGCAGGTATTCTATTGGGACCATCATTCTTAGGAGGAGAAAATGATTTTGCAGAAATAGTTTACCCAGTTAAAAGTTTCTACATCAGCCAAACCTTTGCTTTCTTTGGTTGCATGCTTTTTCTATTCCTAGTGGGTGTTAAAATGGATTTGAGTATAGTCAGACGGTCGGGTAAGAAAGCTGTGGTCATAGGCCTCTCGGCATTTTTCGTCCCTTTGCTTCTAAATGTAGGCTTCGCTCTCGTTCTGCAACGTACGGTCACCATGGAACCCCAGTTGCACAAATCACTTGTAGCCATAGCAGTGTTTCAGTGCCTAAGTAGCTTCCACGTCATCGGTTGTCTCTTGGGCGATTTGAAGCTCCTAAACTCGGAGCTTGGTCGTTTGGCTGTGTCTTCTTCGATGATTAGCGGAGTCATCTCTTGGATTTGGATCATCCTGGCCTTCACAGTACGGCTAAGCACCATGAAAAAGACCGGAAACTTGCCTTTTATGGGTTTAAGTTTAGCCGCATTGTTTGTTCTAATTATATTCATAATGAGGCCAATAATGCTGTGGATGGTTGCCCAAACAGCTGAAGGCAAACCCATTAAAGAGAGCTACATAGTCTCCATCTTCGTCATGGTCATGGTTTGTTCCCTTCTCGGTGAGATTGTGGGCCAGCATTTTTTGCTTGGGCCTATGGTTTTGGGCCTCACAGTGCCGGACGGCCCGCCTTTGGGGTCAGCATTGGTGGAGAAACTTGACTCGTACGTTTCCAACATTCTGTTGCCGAGCTACTTTCTGTTCAGTGGTGCAAGAATCAATTTGAGTGAAATCAGAATGAAGACTGTTGGGATTGTGGAGGTGCTAGCTCTCACTAGCTTTTGTGGTAAAGTATTAGGGACCATGGTGCCTTCACTCTATTGCAAAATGCCTGCTATCGACGCACTTTCATTAGGGCTCATCATGAGTGCCCAAGGCATCACCGACCTTCTTGTCTTGCAACATGGAATGCTACTCTTGCTCATTGACGCAGAATGCTACAGCATCATGATTATGTCATCGGTTTTATTTACTGGAATCATCACCCCCATAGTGAAATTTCTGTACAACCCCTCTAAGAGATACAAGTCCACAAACCGAAGACGAACCATTGAGCATTCCTTGCCAAACATGGAACTCGGACTTCTTGTCTGCATGTTCCACCAAGACAGCACTCCCTCCATCATCAACCTAATAGAAGTCTTCAATCCAACCCCCAAGAGCCCCATTTGCTTCTGCGTTGTCCACCTCATCCGTCTCTCAGGTCGAACAGCTCCAGTCCTCATAACCCACAGACCCGGGAAAAGCACCTCTTTACACTCAAACAACTACTCTGATCACATAATCAATGCCTTCAGATTGTACCAAGAACACAATGCCAGCAGTGTCATAATGAATGCATTCACCGCAATTGCGCCCTATGCCACAATGCACGACGAGGTTTGCACACTTGCTTTTGAAAAAAGGACTTCCATGATAATAATCCCATTTCACAAGTTTTTCAGTGCTATCCAATGCACCGAAGAATTAGCGCATCCAATCAGGTCTGTCAACAGAAATATTCTTCAAAATGCTCCGTGCTCCGTTGGGATTCTTGTAGACCGAGGTACGTTGAATAAAAAACCATCCGCTTTACACACTTCTATATCAGGAAGAAGTTTTTACAACGTTGGGACGATCTTTGTTGAGGGACCTGATGATCGCGAAGCATTGGCGTTAGCGACGCGGATGACTGGAAACCCCAATGTCAGCCTAACCGTTACCAAATTTACCGATCCCAAGCAAAATAGAACCCAGAAAGAGATCGATTCGGAAGTTATAATGAAGTATAAGACAGCCATTGATGGCAAAAAACAGCATGTCTACAAAGAGGTACACGTGAAAGATAGCGTCGACATGATCAATGAGATTAGATCGATGGAGGATTTTTTCGACCTAATTCTGGTGGGGAGAAGACATGATAGTGATTCGCCGTTGTTCATGGGACTTACCGAATGGAATGAGTTTCCGGAACTAGGGTTCCTCGGAGACATGTTAGCATCTTTTGATGCAAATTGCGAGGTGTCAGTGCTGGTGGTGCAGCAACAATCGTTTGGATGTGCTGAAAAAGAGAAGACTGATATCTTTAAATGCCATGTGAACGATTCGTTTTCTGTTGTGGATATACCTCATAATCGACATTAA

Protein Analysis

799

Amino Acids

88.47

Weight (kDa)

8.33

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 42 - 423 4.2e-35 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 484 - 608 7.5e-16 Plant cation/H(+) antiporter domain
CHX17_C PF23259 628 - 767 7.8e-11 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014704)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39830 FvH4_3g39830
malus_domestica MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica Prupe.6G047700_v2.0.a1
pyrus_communis pycom11g04890
rosa_chinensis RchiOBHm_Chr5g0071771
rosa_laevigata RLG00000036244
rosa_multiflora Rmu_sc0001113.1_g000001
rosa_roxburghii Rroxscaffold_1G00009450
rosa_rugosa Rorug05G0414400 Rorug05G0414500
rosa_samantha Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana Rw5G043720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2033
AasI GACNNNNNNGTC 1 cut(s) 1166
Acc36I ACCTGC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 1081
AccI GTMKAC 3 cut(s) 1812, 2076, 2106
AccII CGCG 2 cut(s) 1917, 1939
AccIII TCCGGA 1 cut(s) 2221
AciI CCGC 6 cut(s) 600, 699, 902, 1620, 1842, 1939
AclI AACGTT 1 cut(s) 1881
AclWI GGATC 2 cut(s) 629, 1982
AcsI RAATTY 2 cut(s) 1271, 1979
AcuI CTGAAG 2 cut(s) 786, 1552
AcvI CACGTG 1 cut(s) 2092
AfaI GTAC 7 cut(s) 445, 642, 941, 1280, 1577, 1822, 2088
AfiI CCNNNNNNNGG 6 cut(s) 559, 683, 907, 908, 2155, 2228
AflIII ACRYGT 2 cut(s) 2089, 2244
AgsI TTSAA 6 cut(s) 79, 547, 1420, 1673, 1781, 1828
AhdI GACNNNNNGTC 1 cut(s) 1361
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 1 cut(s) 627
AloI GAACNNNNNNTCC 4 cut(s) 2216, 2217, 2248, 2249
Alw21I GWGCWC 1 cut(s) 1796
Alw26I GTCTC 5 cut(s) 20, 536, 802, 1475, 2235
AlwI GGATC 2 cut(s) 629, 1982
Ama87I CYCGRG 1 cut(s) 1511
Aor13HI TCCGGA 1 cut(s) 2221
AoxI GGCC 7 cut(s) 382, 630, 731, 847, 866, 880, 898
ApeKI GCWGC 2 cut(s) 119, 2293
ApoI RAATTY 2 cut(s) 1271, 1979
Asp700I GAANNNNTTC 1 cut(s) 1613
AspLEI GCGC 2 cut(s) 1630, 1747
AspS9I GGNCC 7 cut(s) 209, 847, 866, 880, 899, 1074, 1904
AsuC2I CCSGG 2 cut(s) 1512, 1513
AsuHPI GGTGA 7 cut(s) 77, 121, 442, 848, 1147, 1249, 1609
AsuNHI GCTAGC 1 cut(s) 1039
AvaI CYCGRG 1 cut(s) 1511
AvaII GGWCC 3 cut(s) 209, 1074, 1904
BaeGI GKGCMC 1 cut(s) 1147
BanI GGYRCC 1 cut(s) 1081
BanII GRGCYC 2 cut(s) 1134, 1439
BbrPI CACGTG 1 cut(s) 2092
BbsI GAAGAC 6 cut(s) 576, 1022, 1321, 1408, 2173, 2333
Bbv12I GWGCWC 1 cut(s) 1796
BbvI GCAGC 2 cut(s) 106, 2305
BccI CCATC 8 cut(s) 94, 220, 742, 809, 1403, 1846, 2051, 2125
BceAI ACGGC 3 cut(s) 659, 913, 2170
BciT130I CCWGG 1 cut(s) 629
BclI TGATCA 2 cut(s) 1549, 2112
BcnI CCSGG 2 cut(s) 1512, 1513
BcoDI GTCTC 5 cut(s) 20, 536, 802, 1475, 2235
BfaI CTAG 4 cut(s) 320, 1040, 1050, 2228
BfmI CTRYAG 2 cut(s) 114, 1213
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 3 cut(s) 120, 699, 2294
BlpI GCTNAGC 1 cut(s) 647
BlsI GCNGC 3 cut(s) 121, 700, 2295
Bme1390I CCNGG 3 cut(s) 629, 1512, 1513
Bme18I GGWCC 3 cut(s) 209, 1074, 1904
BmeRI GACNNNNNGTC 1 cut(s) 1361
BmeT110I CYCGRG 1 cut(s) 1511
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 7 cut(s) 209, 847, 866, 880, 899, 1074, 1904
BmiI GGNNCC 6 cut(s) 210, 459, 1075, 1083, 1905, 2234
BmrFI CCNGG 3 cut(s) 629, 1512, 1513
BmrI ACTGGG 2 cut(s) 251, 457
BmsI GCATC 5 cut(s) 1161, 1227, 1756, 2251, 2261
BmtI GCTAGC 1 cut(s) 1043
BmuI ACTGGG 2 cut(s) 251, 457
BpiI GAAGAC 6 cut(s) 576, 1022, 1321, 1408, 2173, 2333
BpmI CTGGAG 1 cut(s) 1473
Bpu1102I GCTNAGC 1 cut(s) 647
BpuMI CCSGG 2 cut(s) 1512, 1513
Bsa29I ATCGAT 2 cut(s) 2019, 2129
BsaAI YACGTR 1 cut(s) 2092
BsaBI GATNNNNATC 2 cut(s) 620, 1694
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 7 cut(s) 123, 453, 1077, 1147, 1511, 1816, 2236
BsaWI WCCGGW 2 cut(s) 665, 2221
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 6 cut(s) 559, 683, 907, 908, 2155, 2228
Bse1I ACTGG 6 cut(s) 20, 257, 463, 1252, 1490, 1951
Bse8I GATNNNNATC 2 cut(s) 620, 1694
BseAI TCCGGA 1 cut(s) 2221
BseBI CCWGG 1 cut(s) 629
BseCI ATCGAT 2 cut(s) 2019, 2129
BseDI CCNNGG 7 cut(s) 123, 453, 1077, 1147, 1511, 1816, 2236
BseGI GGATG 7 cut(s) 624, 753, 1464, 1747, 1838, 1947, 2315
BseJI GATNNNNATC 2 cut(s) 620, 1694
BseLI CCNNNNNNNGG 6 cut(s) 559, 683, 907, 908, 2155, 2228
BseMII CTCAG 1 cut(s) 1488
BseNI ACTGG 6 cut(s) 20, 257, 463, 1252, 1490, 1951
BseRI GAGGAG 1 cut(s) 240
BseSI GKGCMC 1 cut(s) 1147
BseXI GCAGC 2 cut(s) 106, 2305
BsgI GTGCAG 1 cut(s) 2312
Bsh1236I CGCG 2 cut(s) 1917, 1939
Bsh1285I CGRYCG 1 cut(s) 359
BshFI GGCC 7 cut(s) 384, 632, 733, 849, 868, 882, 900
BshNI GGYRCC 1 cut(s) 1081
BshVI ATCGAT 2 cut(s) 2019, 2129
BsiEI CGRYCG 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 1796
BsiHKCI CYCGRG 1 cut(s) 1511
BsiSI CCGG 4 cut(s) 666, 893, 1512, 2222
BsiWI CGTACG 2 cut(s) 443, 939
BslFI GGGAC 6 cut(s) 222, 386, 1087, 1900, 1917, 2211
BslI CCNNNNNNNGG 6 cut(s) 559, 683, 907, 908, 2155, 2228
BsmAI GTCTC 5 cut(s) 20, 536, 802, 1475, 2235
BsmBI CGTCTC 1 cut(s) 1475
BsmFI GGGAC 6 cut(s) 222, 386, 1087, 1900, 1917, 2211
BsmI GAATGC 5 cut(s) 1188, 1214, 1330, 1613, 1615
BsnI GGCC 7 cut(s) 384, 632, 733, 849, 868, 882, 900
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 1511
Bsp1286I GDGCHC 4 cut(s) 1134, 1147, 1439, 1796
Bsp13I TCCGGA 1 cut(s) 2221
Bsp1407I TGTACA 1 cut(s) 1278
Bsp143I GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
Bsp1720I GCTNAGC 1 cut(s) 647
Bsp19I CCATGG 2 cut(s) 453, 1077
Bsp68I TCGCGA 1 cut(s) 1917
BspACI CCGC 6 cut(s) 600, 699, 902, 1620, 1842, 1939
BspANI GGCC 7 cut(s) 384, 632, 733, 849, 868, 882, 900
BspCNI CTCAG 1 cut(s) 1487
BspDI ATCGAT 2 cut(s) 2019, 2129
BspEI TCCGGA 1 cut(s) 2221
BspFNI CGCG 2 cut(s) 1917, 1939
BspHI TCATGA 2 cut(s) 1137, 1221
BspLI GGNNCC 6 cut(s) 210, 459, 1075, 1083, 1905, 2234
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 1 cut(s) 1043
BspPI GGATC 2 cut(s) 629, 1982
BspT107I GGYRCC 1 cut(s) 1081
BspTNI GGTCTC 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 1278
BsrI ACTGG 6 cut(s) 20, 257, 463, 1252, 1490, 1951
BssECI CCNNGG 7 cut(s) 123, 453, 1077, 1147, 1511, 1816, 2236
BssMI GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
BssT1I CCWWGG 4 cut(s) 123, 453, 1077, 1147
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 6 cut(s) 357, 448, 640, 889, 1021, 1972
Bst6I CTCTTC 1 cut(s) 143
BstAPI GCANNNNNTGC 2 cut(s) 859, 1336
BstAUI TGTACA 1 cut(s) 1278
BstBAI YACGTR 1 cut(s) 2092
BstC8I GCNNGC 7 cut(s) 192, 305, 851, 902, 1041, 1107, 1594
BstDEI CTNAG 5 cut(s) 220, 506, 647, 1290, 1474
BstDSI CCRYGG 2 cut(s) 453, 1077
BstEII GGTNACC 2 cut(s) 127, 448
BstF5I GGATG 7 cut(s) 624, 753, 1464, 1747, 1838, 1947, 2315
BstFNI CGCG 2 cut(s) 1917, 1939
BstHHI GCGC 2 cut(s) 1630, 1747
BstKTI GATC 9 cut(s) 47, 624, 1552, 1893, 1915, 1990, 2019, 2115, 2129
BstMAI GTCTC 5 cut(s) 20, 536, 802, 1475, 2235
BstMBI GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
BstMCI CGRYCG 1 cut(s) 359
BstNI CCWGG 1 cut(s) 629
BstNSI RCATGY 4 cut(s) 307, 1372, 2075, 2248
BstPI GGTNACC 2 cut(s) 127, 448
BstSCI CCNGG 3 cut(s) 627, 1510, 1511
BstSFI CTRYAG 2 cut(s) 114, 1213
BstSLI GKGCMC 1 cut(s) 1147
BstUI CGCG 2 cut(s) 1917, 1939
BstV1I GCAGC 2 cut(s) 106, 2305
BstV2I GAAGAC 6 cut(s) 576, 1022, 1321, 1408, 2173, 2333
Bsu15I ATCGAT 2 cut(s) 2019, 2129
BsuRI GGCC 7 cut(s) 384, 632, 733, 849, 868, 882, 900
BsuTUI ATCGAT 2 cut(s) 2019, 2129
BtgI CCRYGG 2 cut(s) 453, 1077
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 7 cut(s) 624, 753, 1464, 1747, 1838, 1947, 2315
BtsI GCAGTG 2 cut(s) 498, 1603
BtsIMutI CAGTG 7 cut(s) 498, 506, 894, 985, 1603, 1723, 2286
BtuMI TCGCGA 1 cut(s) 1917
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 7 cut(s) 192, 305, 851, 902, 1041, 1107, 1594
CciI TCATGA 2 cut(s) 1137, 1221
CfoI GCGC 2 cut(s) 1630, 1747
Cfr13I GGNCC 7 cut(s) 209, 847, 866, 880, 899, 1074, 1904
Cfr9I CCCGGG 1 cut(s) 1511
ClaI ATCGAT 2 cut(s) 2019, 2129
CseI GACGC 4 cut(s) 1124, 1211, 1945, 2092
Csp6I GTAC 7 cut(s) 444, 641, 940, 1279, 1576, 1821, 2087
CviQI GTAC 7 cut(s) 444, 641, 940, 1279, 1576, 1821, 2087
DdeI CTNAG 5 cut(s) 220, 506, 647, 1290, 1474
DpnI GATC 9 cut(s) 46, 623, 1551, 1892, 1914, 1989, 2018, 2114, 2128
DpnII GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
DraI TTTAAA 1 cut(s) 2341
DrdI GACNNNNNNGTC 1 cut(s) 1166
DriI GACNNNNNGTC 1 cut(s) 1361
DseDI GACNNNNNNGTC 1 cut(s) 1166
Eam1104I CTCTTC 1 cut(s) 143
Eam1105I GACNNNNNGTC 1 cut(s) 1361
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 4 cut(s) 123, 453, 1077, 1147
Eco147I AGGCCT 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 1134, 1439
Eco31I GGTCTC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 2335
Eco47I GGWCC 3 cut(s) 209, 1074, 1904
Eco57I CTGAAG 2 cut(s) 786, 1552
Eco72I CACGTG 1 cut(s) 2092
Eco88I CYCGRG 1 cut(s) 1511
Eco91I GGTNACC 2 cut(s) 127, 448
EcoO109I RGGNCCY 1 cut(s) 1904
EcoO65I GGTNACC 2 cut(s) 127, 448
EcoRII CCWGG 1 cut(s) 627
EcoRV GATATC 1 cut(s) 2335
EcoT14I CCWWGG 4 cut(s) 123, 453, 1077, 1147
EcoT22I ATGCAT 1 cut(s) 1615
EcoT38I GRGCYC 2 cut(s) 1134, 1439
ErhI CCWWGG 4 cut(s) 123, 453, 1077, 1147
Esp3I CGTCTC 1 cut(s) 1475
FalI AAGNNNNNCTT 2 cut(s) 1510, 1542
FaqI GGGAC 6 cut(s) 222, 386, 1087, 1900, 1917, 2211
FauI CCCGC 1 cut(s) 909
FbaI TGATCA 2 cut(s) 1549, 2112
FblI GTMKAC 3 cut(s) 1812, 2076, 2106
Fnu4HI GCNGC 3 cut(s) 120, 699, 2294
FokI GGATG 7 cut(s) 611, 760, 1451, 1734, 1825, 1954, 2322
FriOI GRGCYC 2 cut(s) 1134, 1439
Fsp4HI GCNGC 3 cut(s) 120, 699, 2294
FspBI CTAG 4 cut(s) 320, 1040, 1050, 2228
GlaI GCGC 2 cut(s) 1629, 1746
GluI GCNGC 3 cut(s) 120, 699, 2294
GsuI CTGGAG 1 cut(s) 1473
HaeIII GGCC 7 cut(s) 384, 632, 733, 849, 868, 882, 900
HapII CCGG 4 cut(s) 666, 893, 1512, 2222
HgaI GACGC 4 cut(s) 1124, 1211, 1945, 2092
HhaI GCGC 2 cut(s) 1630, 1747
Hin6I GCGC 2 cut(s) 1628, 1745
HinP1I GCGC 2 cut(s) 1628, 1745
HincII GTYRAC 2 cut(s) 1765, 2107
HindII GTYRAC 2 cut(s) 1765, 2107
HinfI GANTC 8 cut(s) 603, 935, 990, 1251, 1804, 2021, 2180, 2357
HpaII CCGG 4 cut(s) 666, 893, 1512, 2222
HphI GGTGA 7 cut(s) 77, 121, 442, 848, 1147, 1249, 1609
Hpy166II GTNNAC 9 cut(s) 253, 1305, 1459, 1765, 1813, 2077, 2089, 2107, 2353
Hpy188I TCNGA 9 cut(s) 164, 353, 562, 1010, 1355, 1549, 1571, 2026, 2240
Hpy188III TCNNGA 5 cut(s) 1138, 1222, 1863, 1916, 2222
Hpy8I GTNNAC 9 cut(s) 253, 1305, 1459, 1765, 1813, 2077, 2089, 2107, 2353
Hpy99I CGWCG 4 cut(s) 1118, 1652, 1939, 2108
HpyAV CCTTC 6 cut(s) 643, 761, 839, 1095, 1172, 1576
HpyCH4III ACNGT 6 cut(s) 357, 448, 640, 889, 1021, 1972
HpyCH4IV ACGT 6 cut(s) 442, 519, 942, 1823, 1881, 2091
HpyF3I CTNAG 5 cut(s) 220, 506, 647, 1290, 1474
HpySE526I ACGT 6 cut(s) 442, 519, 942, 1823, 1881, 2091
HspAI GCGC 2 cut(s) 1628, 1745
Kpn2I TCCGGA 1 cut(s) 2221
Ksp22I TGATCA 2 cut(s) 1549, 2112
Kzo9I GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
LmnI GCTCC 5 cut(s) 555, 562, 1492, 1792, 1799
Lsp1109I GCAGC 2 cut(s) 106, 2305
LweI GCATC 5 cut(s) 1161, 1227, 1756, 2251, 2261
MaeI CTAG 4 cut(s) 320, 1040, 1050, 2228
MaeII ACGT 6 cut(s) 442, 519, 942, 1823, 1881, 2091
MaeIII GTNAC 3 cut(s) 127, 448, 1972
MalI GATC 9 cut(s) 46, 623, 1551, 1892, 1914, 1989, 2018, 2114, 2128
MboI GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
MfeI CAATTG 1 cut(s) 1623
MhlI GDGCHC 4 cut(s) 1134, 1147, 1439, 1796
MluCI AATT 9 cut(s) 143, 714, 994, 1271, 1623, 1739, 1979, 2151, 2266
MlyI GAGTC 2 cut(s) 612, 929
MmeI TCCRAC 2 cut(s) 972, 1448
Mph1103I ATGCAT 1 cut(s) 1615
MroI TCCGGA 1 cut(s) 2221
MroXI GAANNNNTTC 1 cut(s) 1613
MseI TTAA 6 cut(s) 261, 330, 689, 780, 2340, 2395
MslI CAYNNNNRTG 4 cut(s) 491, 815, 1226, 2175
MspA1I CMGCKG 2 cut(s) 119, 764
MspI CCGG 4 cut(s) 666, 893, 1512, 2222
MspR9I CCNGG 3 cut(s) 629, 1512, 1513
MunI CAATTG 1 cut(s) 1623
Mva1269I GAATGC 5 cut(s) 1188, 1214, 1330, 1613, 1615
MvaI CCWGG 1 cut(s) 629
MvnI CGCG 2 cut(s) 1917, 1939
NciI CCSGG 2 cut(s) 1512, 1513
NcoI CCATGG 2 cut(s) 453, 1077
NdeII GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
NheI GCTAGC 1 cut(s) 1039
NlaIV GGNNCC 6 cut(s) 210, 459, 1075, 1083, 1905, 2234
NmeAIII GCCGAG 2 cut(s) 368, 987
NmuCI GTSAC 2 cut(s) 127, 448
NruI TCGCGA 1 cut(s) 1917
NsiI ATGCAT 1 cut(s) 1615
NspI RCATGY 4 cut(s) 307, 1372, 2075, 2248
PaeI GCATGC 1 cut(s) 307
PagI TCATGA 2 cut(s) 1137, 1221
PceI AGGCCT 1 cut(s) 384
PciI ACATGT 1 cut(s) 2244
PctI GAATGC 5 cut(s) 1188, 1214, 1330, 1613, 1615
PdmI GAANNNNTTC 1 cut(s) 1613
PfeI GAWTC 6 cut(s) 990, 1251, 1804, 2021, 2180, 2357
Pfl23II CGTACG 2 cut(s) 443, 939
PkrI GCNGC 3 cut(s) 121, 700, 2295
PleI GAGTC 2 cut(s) 611, 929
PmaCI CACGTG 1 cut(s) 2092
PmlI CACGTG 1 cut(s) 2092
PpsI GAGTC 2 cut(s) 611, 929
Ppu21I YACGTR 1 cut(s) 2092
PpuMI RGGWCCY 1 cut(s) 1904
PscI ACATGT 1 cut(s) 2244
PsiI TTATAA 1 cut(s) 2033
Psp1406I AACGTT 1 cut(s) 1881
Psp5II RGGWCCY 1 cut(s) 1904
Psp6I CCWGG 1 cut(s) 627
PspCI CACGTG 1 cut(s) 2092
PspEI GGTNACC 2 cut(s) 127, 448
PspGI CCWGG 1 cut(s) 627
PspLI CGTACG 2 cut(s) 443, 939
PspN4I GGNNCC 6 cut(s) 210, 459, 1075, 1083, 1905, 2234
PspPI GGNCC 7 cut(s) 209, 847, 866, 880, 899, 1074, 1904
PspPPI RGGWCCY 1 cut(s) 1904
PvuII CAGCTG 2 cut(s) 119, 764
RruI TCGCGA 1 cut(s) 1917
RsaI GTAC 7 cut(s) 445, 642, 941, 1280, 1577, 1822, 2088
RsaNI GTAC 7 cut(s) 444, 641, 940, 1279, 1576, 1821, 2087
RseI CAYNNNNRTG 4 cut(s) 491, 815, 1226, 2175
SalI GTCGAC 1 cut(s) 2105
SaqAI TTAA 6 cut(s) 261, 330, 689, 780, 2340, 2395
SatI GCNGC 3 cut(s) 120, 699, 2294
Sau3AI GATC 9 cut(s) 44, 621, 1549, 1890, 1912, 1987, 2016, 2112, 2126
Sau96I GGNCC 7 cut(s) 209, 847, 866, 880, 899, 1074, 1904
SchI GAGTC 2 cut(s) 612, 929
ScrFI CCNGG 3 cut(s) 629, 1512, 1513
SduI GDGCHC 4 cut(s) 1134, 1147, 1439, 1796
SfaNI GCATC 5 cut(s) 1161, 1227, 1756, 2251, 2261
SfcI CTRYAG 2 cut(s) 114, 1213
SinI GGWCC 3 cut(s) 209, 1074, 1904
SmaI CCCGGG 1 cut(s) 1513
SmiMI CAYNNNNRTG 4 cut(s) 491, 815, 1226, 2175
SphI GCATGC 1 cut(s) 307
Sse9I AATT 9 cut(s) 143, 714, 994, 1271, 1623, 1739, 1979, 2151, 2266
SseBI AGGCCT 1 cut(s) 384
SsiI CCGC 6 cut(s) 600, 699, 902, 1620, 1842, 1939
SspI AATATT 1 cut(s) 1774
SspMI CTAG 4 cut(s) 320, 1040, 1050, 2228
StuI AGGCCT 1 cut(s) 384
StyD4I CCNGG 3 cut(s) 627, 1510, 1511
StyI CCWWGG 4 cut(s) 123, 453, 1077, 1147
TaaI ACNGT 6 cut(s) 357, 448, 640, 889, 1021, 1972
TaiI ACGT 6 cut(s) 445, 522, 945, 1826, 1884, 2094
TaqI TCGA 9 cut(s) 47, 590, 1113, 1480, 2019, 2106, 2129, 2145, 2389
TaqII GACCGA 1 cut(s) 1830
TasI AATT 9 cut(s) 143, 714, 994, 1271, 1623, 1739, 1979, 2151, 2266
TatI WGTACW 1 cut(s) 1278
TauI GCSGC 1 cut(s) 701
TfiI GAWTC 6 cut(s) 990, 1251, 1804, 2021, 2180, 2357
Tru1I TTAA 6 cut(s) 261, 330, 689, 780, 2340, 2395
Tru9I TTAA 6 cut(s) 261, 330, 689, 780, 2340, 2395
TscAI CASTG 7 cut(s) 498, 506, 894, 985, 1603, 1723, 2286
TseFI GTSAC 2 cut(s) 127, 448
TseI GCWGC 2 cut(s) 119, 2293
Tsp45I GTSAC 2 cut(s) 127, 448
TspDTI ATGAA 9 cut(s) 17, 136, 671, 712, 1028, 1113, 1622, 2051, 2182
TspGWI ACGGA 3 cut(s) 1457, 1779, 1786
TspMI CCCGGG 1 cut(s) 1511
TspRI CASTG 7 cut(s) 498, 506, 894, 985, 1603, 1723, 2286
VpaK11BI GGWCC 3 cut(s) 209, 1074, 1904
XapI RAATTY 2 cut(s) 1271, 1979
XceI RCATGY 4 cut(s) 307, 1372, 2075, 2248
XcmI CCANNNNNNNNNTGG 2 cut(s) 741, 808
XmaI CCCGGG 1 cut(s) 1511
XmiI GTMKAC 3 cut(s) 1812, 2076, 2106
XmnI GAANNNNTTC 1 cut(s) 1613
XspI CTAG 4 cut(s) 320, 1040, 1050, 2228
Zsp2I ATGCAT 1 cut(s) 1615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.