Rroxscaffold_1G00009450

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014704
Physical Location & Seq
Forward (+)
11952870 .. 11955122
2253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009450.1

Sequence Viewer

Length: 1188 bp
ATGGTCATGATTTGTTCCCTTCTCGGTGAGATTGTGGGCCAGCATTTTTTGCTTGGGCCTATGGTTTTGGGCCTCGCCGTGCCGGACGGCCCGCCTTTGGGGTCAGCATTGGTGGAGAAACTTGACTCGTACGTTTCCAACATTCTGTTGCCGAGCTACTTTCTGTTCACTGGTGCAAGAATCAATTTGAGTGAAATCAGAATGAAGACTGTTGGGATTGTGGAGGTGCTGGCTCTCAGTAGCTTTTGTGGTAAAGTATTAGGGACCATGGTGCCTTCACTCTATTGCAAAATGCCTGCTATCGACGCACTTTCATTAGGGCTCATCATGAGTGCCCAAGGCATCACCGACCTTCTTGTCTTGCAACATGGAATGCTACTCTTGCTCATTGACGGAGAATGCTACAGCATCATGATTATGTCATCGGTTTTATTTACTGGAATCATCACCCCCATAGTGAAATTTCTGTACAACCCCTCTAAGAGATACAAGTCCACAAAGCGAAGACGAACCATTGAGCATTCCTTGCCAAACATGGAACTCGGACTTCTTGTCTGCATGTTCCACCAAGACAGCACTCCCTCCATCATCAACCTAATAGAAGTCTTCAATCCAACCCCCAAGAACCCCATTCGCTTCTGCGTTGTCCACCTCATCCGTCTCTCAGGTCGAACAGCTCCAGTCCTCATAACCCACAGACCCGGGGAAAAGCACCTCTTTAAACTCAAACAACTGCTCCGATCACATAATCAATGCCTTCAGATTGTACCAAGAACACAATGCCAGCAGTGTCATAATGAATGCATTCACCGCAATTGCGCCCTATGCCACAATGCACGACGAGGAAGAAGTTTGTACAGCGTTGGGATGATCTTTGTTGAGGGACCTGATGACCGCGAAGCATTGGCGTTAGCGACGCGGATGGCTGGAAACCCCAATGTCAGCCTAACCGTTACCAAATTTACCGATCCCAAGCAAAATAGAACCCAGAAAGAGATCGATTCGGAAGTTATAATGAAGTATAAGACAGCCATTGATGGCAAAAAACAGCATGTCTACAAAGAGGTGTCAGTGCTGGTGGTGCAACAACAATCATTTGGATGTGCTGAAAAAGAGAAGACTGATATCTTTAAATGCCATGTGAAGGATTCGTTTTCTGTTGTGGATATACCTCATAATCGACATTAA

Protein Analysis

395

Amino Acids

44.12

Weight (kDa)

9.13

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 1 - 153 1.3e-14 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014704)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39830 FvH4_3g39830
malus_domestica MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica Prupe.6G047700_v2.0.a1
pyrus_communis pycom11g04890
rosa_chinensis RchiOBHm_Chr5g0071771
rosa_laevigata RLG00000036244
rosa_multiflora Rmu_sc0001113.1_g000001
rosa_roxburghii Rroxscaffold_1G00009450
rosa_rugosa Rorug05G0414400 Rorug05G0414500
rosa_samantha Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana Rw5G043720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1013
AasI GACNNNNNNGTC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 271
AccI GTMKAC 1 cut(s) 1056
AccII CGCG 2 cut(s) 897, 919
AciI CCGC 4 cut(s) 92, 811, 895, 919
AclWI GGATC 1 cut(s) 962
AcsI RAATTY 2 cut(s) 461, 959
AcuI CTGAAG 1 cut(s) 743
AfaI GTAC 4 cut(s) 131, 470, 768, 857
AfiI CCNNNNNNNGG 3 cut(s) 97, 98, 1144
AgsI TTSAA 1 cut(s) 610
AhdI GACNNNNNGTC 1 cut(s) 551
AluBI AGCT 3 cut(s) 156, 243, 677
AluI AGCT 3 cut(s) 156, 243, 677
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 1 cut(s) 962
Ama87I CYCGRG 1 cut(s) 701
AoxI GGCC 4 cut(s) 37, 56, 70, 88
ApoI RAATTY 2 cut(s) 461, 959
Asp700I GAANNNNTTC 1 cut(s) 804
AspLEI GCGC 1 cut(s) 821
AspS9I GGNCC 6 cut(s) 37, 56, 70, 89, 264, 884
AsuC2I CCSGG 2 cut(s) 702, 703
AsuHPI GGTGA 4 cut(s) 38, 337, 439, 800
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 2 cut(s) 264, 884
BaeGI GKGCMC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 1 cut(s) 324
BbsI GAAGAC 4 cut(s) 212, 511, 598, 1124
BccI CCATC 3 cut(s) 593, 916, 1031
BceAI ACGGC 2 cut(s) 62, 103
BcnI CCSGG 2 cut(s) 702, 703
BcoDI GTCTC 1 cut(s) 665
BfmI CTRYAG 1 cut(s) 403
Bme1390I CCNGG 2 cut(s) 702, 703
Bme18I GGWCC 2 cut(s) 264, 884
BmeRI GACNNNNNGTC 1 cut(s) 551
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 6 cut(s) 37, 56, 70, 89, 264, 884
BmiI GGNNCC 3 cut(s) 265, 273, 885
BmrFI CCNGG 2 cut(s) 702, 703
BmsI GCATC 2 cut(s) 351, 417
BpiI GAAGAC 4 cut(s) 212, 511, 598, 1124
BpmI CTGGAG 1 cut(s) 663
BpuMI CCSGG 2 cut(s) 702, 703
Bsa29I ATCGAT 1 cut(s) 999
BsaJI CCNNGG 4 cut(s) 267, 337, 701, 702
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 98, 1144
Bse1I ACTGG 3 cut(s) 175, 442, 680
BseCI ATCGAT 1 cut(s) 999
BseDI CCNNGG 4 cut(s) 267, 337, 701, 702
BseGI GGATG 4 cut(s) 654, 873, 927, 1106
BseLI CCNNNNNNNGG 3 cut(s) 97, 98, 1144
BseMII CTCAG 2 cut(s) 250, 678
BseNI ACTGG 3 cut(s) 175, 442, 680
BseSI GKGCMC 1 cut(s) 337
Bsh1236I CGCG 2 cut(s) 897, 919
BshFI GGCC 4 cut(s) 39, 58, 72, 90
BshNI GGYRCC 1 cut(s) 271
BshVI ATCGAT 1 cut(s) 999
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 2 cut(s) 83, 702
BsiWI CGTACG 1 cut(s) 129
BslFI GGGAC 2 cut(s) 277, 897
BslI CCNNNNNNNGG 3 cut(s) 97, 98, 1144
BsmAI GTCTC 1 cut(s) 665
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 2 cut(s) 277, 897
BsmI GAATGC 5 cut(s) 378, 404, 520, 804, 806
BsnI GGCC 4 cut(s) 39, 58, 72, 90
BsoBI CYCGRG 1 cut(s) 701
Bsp1286I GDGCHC 2 cut(s) 324, 337
Bsp1407I TGTACA 2 cut(s) 468, 855
Bsp143I GATC 4 cut(s) 740, 870, 967, 996
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 4 cut(s) 92, 811, 895, 919
BspANI GGCC 4 cut(s) 39, 58, 72, 90
BspCNI CTCAG 2 cut(s) 249, 677
BspDI ATCGAT 1 cut(s) 999
BspFNI CGCG 2 cut(s) 897, 919
BspHI TCATGA 3 cut(s) 6, 327, 411
BspLI GGNNCC 3 cut(s) 265, 273, 885
BspPI GGATC 1 cut(s) 962
BspT107I GGYRCC 1 cut(s) 271
BsrGI TGTACA 2 cut(s) 468, 855
BsrI ACTGG 3 cut(s) 175, 442, 680
BssECI CCNNGG 4 cut(s) 267, 337, 701, 702
BssMI GATC 4 cut(s) 740, 870, 967, 996
BssT1I CCWWGG 2 cut(s) 267, 337
Bst4CI ACNGT 2 cut(s) 211, 952
BstAPI GCANNNNNTGC 2 cut(s) 49, 526
BstAUI TGTACA 2 cut(s) 468, 855
BstC8I GCNNGC 5 cut(s) 41, 92, 231, 297, 785
BstDEI CTNAG 3 cut(s) 236, 480, 664
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 4 cut(s) 654, 873, 927, 1106
BstFNI CGCG 2 cut(s) 897, 919
BstHHI GCGC 1 cut(s) 821
BstKTI GATC 4 cut(s) 743, 873, 970, 999
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 4 cut(s) 740, 870, 967, 996
BstMWI GCNNNNNNNGC 7 cut(s) 49, 305, 382, 526, 810, 825, 1081
BstNSI RCATGY 2 cut(s) 562, 1055
BstSCI CCNGG 2 cut(s) 700, 701
BstSFI CTRYAG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 2 cut(s) 897, 919
BstV2I GAAGAC 4 cut(s) 212, 511, 598, 1124
Bsu15I ATCGAT 1 cut(s) 999
BsuRI GGCC 4 cut(s) 39, 58, 72, 90
BsuTUI ATCGAT 1 cut(s) 999
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 4 cut(s) 654, 873, 927, 1106
BtsI GCAGTG 1 cut(s) 794
BtsIMutI CAGTG 3 cut(s) 168, 794, 1077
Cac8I GCNNGC 5 cut(s) 41, 92, 231, 297, 785
CciI TCATGA 3 cut(s) 6, 327, 411
CfoI GCGC 1 cut(s) 821
Cfr13I GGNCC 6 cut(s) 37, 56, 70, 89, 264, 884
Cfr9I CCCGGG 1 cut(s) 701
ClaI ATCGAT 1 cut(s) 999
CseI GACGC 2 cut(s) 314, 925
Csp6I GTAC 4 cut(s) 130, 469, 767, 856
CviAII CATG 9 cut(s) 7, 268, 328, 368, 412, 535, 559, 1052, 1139
CviQI GTAC 4 cut(s) 130, 469, 767, 856
DdeI CTNAG 3 cut(s) 236, 480, 664
DpnI GATC 4 cut(s) 742, 872, 969, 998
DpnII GATC 4 cut(s) 740, 870, 967, 996
DraI TTTAAA 2 cut(s) 721, 1132
DrdI GACNNNNNNGTC 1 cut(s) 356
DriI GACNNNNNGTC 1 cut(s) 551
DseDI GACNNNNNNGTC 1 cut(s) 356
Eam1105I GACNNNNNGTC 1 cut(s) 551
Eco130I CCWWGG 2 cut(s) 267, 337
Eco24I GRGCYC 1 cut(s) 324
Eco32I GATATC 1 cut(s) 1126
Eco47I GGWCC 2 cut(s) 264, 884
Eco57I CTGAAG 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 701
EcoO109I RGGNCCY 1 cut(s) 884
EcoRV GATATC 1 cut(s) 1126
EcoT14I CCWWGG 2 cut(s) 267, 337
EcoT22I ATGCAT 1 cut(s) 806
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 2 cut(s) 267, 337
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 9 cut(s) 10, 271, 331, 371, 415, 538, 562, 1055, 1142
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 2 cut(s) 277, 897
FatI CATG 9 cut(s) 6, 267, 327, 367, 411, 534, 558, 1051, 1138
FauI CCCGC 1 cut(s) 99
FblI GTMKAC 1 cut(s) 1056
FokI GGATG 4 cut(s) 641, 880, 934, 1113
FriOI GRGCYC 1 cut(s) 324
GlaI GCGC 1 cut(s) 820
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 4 cut(s) 39, 58, 72, 90
HapII CCGG 2 cut(s) 83, 702
HgaI GACGC 2 cut(s) 314, 925
HhaI GCGC 1 cut(s) 821
Hin1II CATG 9 cut(s) 10, 271, 331, 371, 415, 538, 562, 1055, 1142
Hin6I GCGC 1 cut(s) 819
HinP1I GCGC 1 cut(s) 819
HinfI GANTC 5 cut(s) 125, 180, 441, 1001, 1148
HpaII CCGG 2 cut(s) 83, 702
HphI GGTGA 4 cut(s) 38, 337, 439, 800
Hpy166II GTNNAC 4 cut(s) 168, 495, 649, 1057
Hpy188I TCNGA 5 cut(s) 200, 545, 740, 762, 1006
Hpy188III TCNNGA 3 cut(s) 7, 328, 412
Hpy8I GTNNAC 4 cut(s) 168, 495, 649, 1057
Hpy99I CGWCG 3 cut(s) 308, 843, 919
HpyAV CCTTC 5 cut(s) 29, 285, 362, 767, 1138
HpyCH4III ACNGT 2 cut(s) 211, 952
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 7 cut(s) 176, 288, 364, 558, 804, 836, 1084
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 305, 382, 526, 810, 825, 1081
HpyF3I CTNAG 3 cut(s) 236, 480, 664
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 9 cut(s) 10, 271, 331, 371, 415, 538, 562, 1055, 1142
HspAI GCGC 1 cut(s) 819
Kzo9I GATC 4 cut(s) 740, 870, 967, 996
LmnI GCTCC 2 cut(s) 682, 741
LweI GCATC 2 cut(s) 351, 417
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 952
MalI GATC 4 cut(s) 742, 872, 969, 998
MboI GATC 4 cut(s) 740, 870, 967, 996
MboII GAAGA 5 cut(s) 217, 516, 598, 858, 1129
MfeI CAATTG 1 cut(s) 814
MhlI GDGCHC 2 cut(s) 324, 337
MluCI AATT 4 cut(s) 184, 461, 814, 959
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 162, 638
Mph1103I ATGCAT 1 cut(s) 806
MroXI GAANNNNTTC 1 cut(s) 804
MseI TTAA 3 cut(s) 720, 1131, 1186
MslI CAYNNNNRTG 2 cut(s) 416, 1099
MspI CCGG 2 cut(s) 83, 702
MspR9I CCNGG 2 cut(s) 702, 703
MunI CAATTG 1 cut(s) 814
Mva1269I GAATGC 5 cut(s) 378, 404, 520, 804, 806
MvnI CGCG 2 cut(s) 897, 919
MwoI GCNNNNNNNGC 7 cut(s) 49, 305, 382, 526, 810, 825, 1081
NciI CCSGG 2 cut(s) 702, 703
NcoI CCATGG 1 cut(s) 267
NdeII GATC 4 cut(s) 740, 870, 967, 996
NlaIII CATG 9 cut(s) 10, 271, 331, 371, 415, 538, 562, 1055, 1142
NlaIV GGNNCC 3 cut(s) 265, 273, 885
NmeAIII GCCGAG 1 cut(s) 177
NsiI ATGCAT 1 cut(s) 806
NspI RCATGY 2 cut(s) 562, 1055
PagI TCATGA 3 cut(s) 6, 327, 411
PctI GAATGC 5 cut(s) 378, 404, 520, 804, 806
PdmI GAANNNNTTC 1 cut(s) 804
PfeI GAWTC 4 cut(s) 180, 441, 1001, 1148
Pfl23II CGTACG 1 cut(s) 129
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 884
PsiI TTATAA 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 884
PspLI CGTACG 1 cut(s) 129
PspN4I GGNNCC 3 cut(s) 265, 273, 885
PspPI GGNCC 6 cut(s) 37, 56, 70, 89, 264, 884
PspPPI RGGWCCY 1 cut(s) 884
RsaI GTAC 4 cut(s) 131, 470, 768, 857
RsaNI GTAC 4 cut(s) 130, 469, 767, 856
RseI CAYNNNNRTG 2 cut(s) 416, 1099
SaqAI TTAA 3 cut(s) 720, 1131, 1186
Sau3AI GATC 4 cut(s) 740, 870, 967, 996
Sau96I GGNCC 6 cut(s) 37, 56, 70, 89, 264, 884
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 702, 703
SduI GDGCHC 2 cut(s) 324, 337
SfaNI GCATC 2 cut(s) 351, 417
SfcI CTRYAG 1 cut(s) 403
SinI GGWCC 2 cut(s) 264, 884
SmaI CCCGGG 1 cut(s) 703
SmiMI CAYNNNNRTG 2 cut(s) 416, 1099
Sse9I AATT 4 cut(s) 184, 461, 814, 959
SsiI CCGC 4 cut(s) 92, 811, 895, 919
StyD4I CCNGG 2 cut(s) 700, 701
StyI CCWWGG 2 cut(s) 267, 337
TaaI ACNGT 2 cut(s) 211, 952
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 303, 670, 999, 1180
TasI AATT 4 cut(s) 184, 461, 814, 959
TatI WGTACW 2 cut(s) 468, 855
TfiI GAWTC 4 cut(s) 180, 441, 1001, 1148
Tru1I TTAA 3 cut(s) 720, 1131, 1186
Tru9I TTAA 3 cut(s) 720, 1131, 1186
TscAI CASTG 3 cut(s) 175, 794, 1077
TspDTI ATGAA 4 cut(s) 218, 303, 813, 1031
TspGWI ACGGA 2 cut(s) 408, 647
TspMI CCCGGG 1 cut(s) 701
TspRI CASTG 3 cut(s) 175, 794, 1077
VpaK11BI GGWCC 2 cut(s) 264, 884
XapI RAATTY 2 cut(s) 461, 959
XceI RCATGY 2 cut(s) 562, 1055
XmaI CCCGGG 1 cut(s) 701
XmiI GTMKAC 1 cut(s) 1056
XmnI GAANNNNTTC 1 cut(s) 804
Zsp2I ATGCAT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.