Rorug05G0414500

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014704
Physical Location & Seq
Reverse (-)
57311113 .. 57312276
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0414500.1

Sequence Viewer

Length: 564 bp
ATGAGAATTCCAACATATGACAACCAGTTGCTGAACTTAGCTGAGGATCTAGACCGACGATTGTTACCTGCATTCGACACTCCTTCAGGTGTACTGCCCCTTGTCACATTTAGCTTGAATGCACTGTCAGGGAGGCACCACAAAGGCGCAGATGTTTGGGTTGGAGATTGGGACTCTAGCAATGCCTATGATTTTATTAATTATATAGTTTCGAAGGGCTACCACATAAATTCATGGGAGTTGGGTAATGAGCTCTGTGGTAATGGTGTTGGAGCTGAACAGTATGGAAAAGACATGATGAAGCTTAAACAAATTGTCAACCAGTTGTACAATGACTCCCACATAAAGCCTGCAATTGTAGGACCTGGAGGATTCTATGATCAAAAGTGGTTTGCCACGCTTCTTCAGGTTTCTGGTTCAGGCGTAGTCAATGTTGTCACTCAGCATCCTTACACTCTGGGTCCAGGTCATATTGAACTATTGGCTTCAGGAACCCTTCCTCTGTTTTACCTAATAGTCTCCAAAGAAGATAATAAAAGGAAAATCCTCTTTATGTACTGGTAA

Protein Analysis

187

Amino Acids

20.96

Weight (kDa)

6.03

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 34 - 170 3.9e-50 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014704)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39830 FvH4_3g39830
malus_domestica MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica Prupe.6G047700_v2.0.a1
pyrus_communis pycom11g04890
rosa_chinensis RchiOBHm_Chr5g0071771
rosa_laevigata RLG00000036244
rosa_multiflora Rmu_sc0001113.1_g000001
rosa_roxburghii Rroxscaffold_1G00009450
rosa_rugosa Rorug05G0414400 Rorug05G0414500
rosa_samantha Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana Rw5G043720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 76
AccB1I GGYRCC 1 cut(s) 135
AclWI GGATC 1 cut(s) 54
AcsI RAATTY 2 cut(s) 6, 229
AcuI CTGAAG 3 cut(s) 69, 389, 471
AfaI GTAC 3 cut(s) 93, 329, 557
AgsI TTSAA 2 cut(s) 118, 476
AjnI CCWGG 2 cut(s) 364, 463
AluBI AGCT 5 cut(s) 41, 114, 253, 275, 304
AluI AGCT 5 cut(s) 41, 114, 253, 275, 304
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 1 cut(s) 54
AlwNI CAGNNNCTG 1 cut(s) 31
ApoI RAATTY 2 cut(s) 6, 229
AseI ATTAAT 1 cut(s) 198
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 362, 461
AsuII TTCGAA 1 cut(s) 212
AvaII GGWCC 2 cut(s) 362, 461
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 255
BbvCI CCTCAGC 1 cut(s) 42
BciT130I CCWGG 2 cut(s) 366, 465
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 2 cut(s) 50, 177
BfuAI ACCTGC 1 cut(s) 76
Bme1390I CCNGG 2 cut(s) 366, 465
Bme18I GGWCC 2 cut(s) 362, 461
BmgT120I GGNCC 2 cut(s) 362, 461
BmiI GGNNCC 3 cut(s) 137, 462, 493
BmrFI CCNGG 2 cut(s) 366, 465
BmsI GCATC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 42
Bpu14I TTCGAA 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bse1I ACTGG 3 cut(s) 25, 322, 563
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 2 cut(s) 366, 465
BseGI GGATG 1 cut(s) 445
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 33, 455
BseNI ACTGG 3 cut(s) 25, 322, 563
BshNI GGYRCC 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 255
BslFI GGGAC 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 2 cut(s) 71, 124
Bsp119I TTCGAA 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 2 cut(s) 46, 379
BspCNI CTCAG 2 cut(s) 34, 454
BspLI GGNNCC 3 cut(s) 137, 462, 493
BspMI ACCTGC 1 cut(s) 76
BspPI GGATC 1 cut(s) 54
BspT104I TTCGAA 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 187
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 3 cut(s) 25, 322, 563
BssMI GATC 2 cut(s) 46, 379
Bst2UI CCWGG 2 cut(s) 366, 465
Bst4CI ACNGT 2 cut(s) 126, 282
BstAUI TGTACA 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 212
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 3 cut(s) 37, 42, 441
BstF5I GGATG 1 cut(s) 445
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 2 cut(s) 49, 382
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 2 cut(s) 46, 379
BstNI CCWGG 2 cut(s) 366, 465
BstSCI CCNGG 2 cut(s) 364, 463
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BtsCI GGATG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 122
BveI ACCTGC 1 cut(s) 76
Cac8I GCNNGC 1 cut(s) 351
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 362, 461
Csp6I GTAC 3 cut(s) 92, 328, 556
CviAII CATG 2 cut(s) 234, 295
CviJI RGCY 8 cut(s) 41, 114, 219, 253, 275, 304, 349, 485
CviKI_1 RGCY 8 cut(s) 41, 114, 219, 253, 275, 304, 349, 485
CviQI GTAC 3 cut(s) 92, 328, 556
DdeI CTNAG 3 cut(s) 37, 42, 441
DpnI GATC 2 cut(s) 48, 381
DpnII GATC 2 cut(s) 46, 379
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 362, 461
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 3 cut(s) 69, 389, 471
EcoICRI GAGCTC 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 6
EcoRII CCWGG 2 cut(s) 364, 463
EcoT38I GRGCYC 1 cut(s) 255
FaeI CATG 2 cut(s) 237, 298
FaqI GGGAC 1 cut(s) 185
FatI CATG 2 cut(s) 233, 294
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 379
FokI GGATG 1 cut(s) 432
FriOI GRGCYC 1 cut(s) 255
FspBI CTAG 2 cut(s) 50, 177
GlaI GCGC 1 cut(s) 148
GsuI CTGGAG 1 cut(s) 387
HhaI GCGC 1 cut(s) 149
Hin1II CATG 2 cut(s) 237, 298
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 3 cut(s) 173, 335, 372
Hpy166II GTNNAC 2 cut(s) 92, 319
Hpy188III TCNNGA 2 cut(s) 50, 489
Hpy8I GTNNAC 2 cut(s) 92, 319
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 3 cut(s) 93, 208, 506
HpyCH4III ACNGT 2 cut(s) 126, 282
HpyCH4V TGCA 3 cut(s) 71, 122, 353
HpyF3I CTNAG 3 cut(s) 37, 42, 441
Hsp92II CATG 2 cut(s) 237, 298
HspAI GCGC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 2 cut(s) 46, 379
LmnI GCTCC 1 cut(s) 272
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 50, 177
MaeIII GTNAC 3 cut(s) 63, 103, 436
MalI GATC 2 cut(s) 48, 381
MboI GATC 2 cut(s) 46, 379
MboII GAAGA 2 cut(s) 395, 539
MfeI CAATTG 1 cut(s) 354
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 5 cut(s) 6, 199, 229, 312, 354
MlyI GAGTC 2 cut(s) 167, 329
MmeI TCCRAC 3 cut(s) 35, 142, 250
MnlI CCTC 5 cut(s) 37, 126, 362, 510, 557
MseI TTAA 2 cut(s) 198, 306
MspR9I CCNGG 2 cut(s) 366, 465
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 2 cut(s) 71, 124
MvaI CCWGG 2 cut(s) 366, 465
NdeI CATATG 1 cut(s) 16
NdeII GATC 2 cut(s) 46, 379
NlaIII CATG 2 cut(s) 237, 298
NlaIV GGNNCC 3 cut(s) 137, 462, 493
NmuCI GTSAC 2 cut(s) 103, 436
NspV TTCGAA 1 cut(s) 212
PctI GAATGC 2 cut(s) 71, 124
PfeI GAWTC 1 cut(s) 372
PleI GAGTC 2 cut(s) 167, 329
PpsI GAGTC 2 cut(s) 167, 329
PpuMI RGGWCCY 1 cut(s) 362
PshBI ATTAAT 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 364, 463
PspGI CCWGG 2 cut(s) 364, 463
PspN4I GGNNCC 3 cut(s) 137, 462, 493
PspPI GGNCC 2 cut(s) 362, 461
PspPPI RGGWCCY 1 cut(s) 362
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 46
RsaI GTAC 3 cut(s) 93, 329, 557
RsaNI GTAC 3 cut(s) 92, 328, 556
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 198, 306
Sau3AI GATC 2 cut(s) 46, 379
Sau96I GGNCC 2 cut(s) 362, 461
SchI GAGTC 2 cut(s) 167, 329
ScrFI CCNGG 2 cut(s) 366, 465
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 1 cut(s) 454
SfuI TTCGAA 1 cut(s) 212
SinI GGWCC 2 cut(s) 362, 461
Sse9I AATT 5 cut(s) 6, 199, 229, 312, 354
SspMI CTAG 2 cut(s) 50, 177
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 364, 463
TaaI ACNGT 2 cut(s) 126, 282
TaqI TCGA 2 cut(s) 75, 212
TaqII GACCGA 1 cut(s) 69
TasI AATT 5 cut(s) 6, 199, 229, 312, 354
TatI WGTACW 3 cut(s) 91, 327, 555
TfiI GAWTC 1 cut(s) 372
Tru1I TTAA 2 cut(s) 198, 306
Tru9I TTAA 2 cut(s) 198, 306
TscAI CASTG 1 cut(s) 129
TseFI GTSAC 2 cut(s) 103, 436
Tsp45I GTSAC 2 cut(s) 103, 436
TspDTI ATGAA 2 cut(s) 222, 314
TspRI CASTG 1 cut(s) 129
VpaK11BI GGWCC 2 cut(s) 362, 461
VspI ATTAAT 1 cut(s) 198
XapI RAATTY 2 cut(s) 6, 229
XbaI TCTAGA 1 cut(s) 49
XspI CTAG 2 cut(s) 50, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.