Rw5G043720

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014704
Physical Location & Seq
Reverse (-)
76677582 .. 76680703
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043720.1

Sequence Viewer

Length: 2109 bp
ATGAATAGGGCAAACTGGATATGCGAGACCCAGAAAGGCATAAGATCGAAAGGTATCTTTTTGGGTGATAATCCTTTGAAATACCCATCTCCAATACTTCTTCTGCAACTTACTATAGCTGCCTTGGTCACCACTCTCTTCCAATTCATTCTCAACCCTCTCGGAGAAAGTGCTTATATCTCACAAATGCTGGCAGGTATTCTATTGGGACCATCATTCTTAGGAGGAGAAAATCATTTTGCAGAAATAGTTTACCCAGTTAAAAGTTTCTACATCAGCCAAACCTTTGCTTTCTTTGGTTGCATGCTTTTTCTATTCCTAGTGGGTGTTAAAATGGATTTGAGTATAGTCCGACGGTCGGGAAAGAAAGCTGTGGTCATAGGCCTCTCGGCATTTTTCGTCCCTTTGGTTCTAAATGTAGGCTTCGCTCTCGTTCTGCAACGTACGGTCACCATGGAACCCCAGTTGCACAAATCACTTGTAGCCATAGCAGTGTTTCAGTGCCTAAGTAGCTTCCACGTCATCGGTTGTCTCTTGGGCGATTTGAAGCTCCTAAACTCGGAGCTTGGTCGTTTGGCTGTGTCTTCTTCGATGATTAGCGGAGTCATCTCTTGGATTTGGGTCATCCTGGCGTTCACAGTACGGCTAAGCACCATGAAAAAGACCGGAAACTTGCCTTTTATGGGTTTAAGTTTAGCCGCGTTGTTTGTTCTAATTATATTCATAATGAGGCCAATAATGCTGTGGATGGTTGCCCAAACAGCTAAAGGCAAACCCATTAAAGAGAGCTACATAGTCTCCATCTTCATAATGGTCATGATTTTATTAGGGACCATGGTGCCTTCACTCTATTGCAAAATGCCTGCTATCGACGCACTTTCATTAGGGCTCATCATGAGTGCCCAAGGCATCACCGACCTTCTTGTCTTGCAACATGGAATGCTACTCTTGCTCATTGACCGAGAATGCTACAGCATCATGATTATGTCATCGGTTTTATTTACTGGAATCATCACCCCCATAGTGAAATTTTTGTACAACCCCTCTAAGAGATACAAGTCCACAAAGCGAAGACGAACCATTGAGCATTCCTTGCCAAACATGGAACTCGGACTTCTTGTCTGCATGTTCCACCAAGACAGCACTCCCTCCATCATAAACCTAATAGAAGTCTTCAATCCAACCCCCAAGAGCCCCATTTGCTTCTGCGTTGTCCACCTCATCCGTCTCTCAGGTCGAACAGCTCCAGTCCTCATAACCCACAGACCCGGGAAAAACACCTCTTTACACTCAAACAACTACTCCGATCACATAATCAATGCCTTCAGATTGTACCAAGAACACAATGCCAGCAGTGTCATAATGAATGCATTCACCGCAATTGCGCCCTATGCCACAATGCACGACGAGGTTTGCACACTTGCTTTTGAAAAAAGGACTTCCATGATAATAATCCCATTTCACAAGTTTTTCAGTGCTATCCAATGCACCGAAGAATTAGAGCATCCAATCAGGTCTGTCAACAGAAATATTCTTCAAAATGCTCCGTGCTCCGTTGGGATTCTTGTAGACCGAGGTACAAGTTTGTACAGTGTTGGGACGATCTTTGTTGAGGGACCTGATGACCGCGAAGCATTGGCGTTAGCGACGCGGATGGCTGGAAATCCCAATGTCAGCCTAACCGTTACCAAATTTACCGATCCCAAGCAAAATAGAACCCAGAAAGAGATCGATTCGGAAGTTATAATGAAGTATAAGACAGCCATTGATGGCAAAAAACAGCATGTCTACAAAGAGGTACATGTTAAAGATAGCGTCGACATGATCAATGAGATTAGATCGATGGAGTATTTTTTCGACCTAATTCTGGTGGGGAGAAGACATGATAGTGATTCGCCGTTGTTCATGGGACTTACCGAATGGAATGAGTTTCCGGAACTAGGGTTCCTCGGAGACATGTTAGCATCTTTTGATGCAAATTGCGAGGTGTCAGTGCTGGTGGTGCAGCAACAATCGTTTGGATGTGCTGAAAAAGAGAAGACTGATATCTTTAAATGCCATGTGAAGGATTCGTTTTCTGTTGTGGATATACCTCATAATCGACATTAA

Protein Analysis

702

Amino Acids

78.49

Weight (kDa)

8.74

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 41 - 277 9e-16 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 403 - 527 2e-16 Plant cation/H(+) antiporter domain
CHX17_C PF23259 531 - 671 8.2e-13 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014704)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39830 FvH4_3g39830
malus_domestica MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica Prupe.6G047700_v2.0.a1
pyrus_communis pycom11g04890
rosa_chinensis RchiOBHm_Chr5g0071771
rosa_laevigata RLG00000036244
rosa_multiflora Rmu_sc0001113.1_g000001
rosa_roxburghii Rroxscaffold_1G00009450
rosa_rugosa Rorug05G0414400 Rorug05G0414500
rosa_samantha Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana Rw5G043720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1745
AasI GACNNNNNNGTC 1 cut(s) 923
Acc36I ACCTGC 1 cut(s) 185
AccB1I GGYRCC 1 cut(s) 838
AccI GTMKAC 3 cut(s) 1569, 1788, 1818
AccII CGCG 3 cut(s) 701, 1629, 1651
AccIII TCCGGA 1 cut(s) 1933
AciI CCGC 5 cut(s) 600, 699, 1377, 1627, 1651
AclWI GGATC 1 cut(s) 1694
AcsI RAATTY 2 cut(s) 1028, 1691
AcuI CTGAAG 1 cut(s) 1309
AfaI GTAC 7 cut(s) 445, 642, 1037, 1334, 1579, 1589, 1800
AfiI CCNNNNNNNGG 6 cut(s) 358, 559, 683, 1867, 1940, 2065
AflIII ACRYGT 2 cut(s) 1801, 1956
AgsI TTSAA 5 cut(s) 79, 547, 1177, 1430, 1538
AhdI GACNNNNNGTC 1 cut(s) 1118
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 1 cut(s) 627
AloI GAACNNNNNNTCC 4 cut(s) 1928, 1929, 1960, 1961
AluBI AGCT 8 cut(s) 119, 371, 513, 550, 565, 764, 789, 1244
AluI AGCT 8 cut(s) 119, 371, 513, 550, 565, 764, 789, 1244
Alw21I GWGCWC 1 cut(s) 1553
Alw26I GTCTC 5 cut(s) 20, 536, 802, 1232, 1947
AlwI GGATC 1 cut(s) 1694
Ama87I CYCGRG 1 cut(s) 1268
Aor13HI TCCGGA 1 cut(s) 1933
AoxI GGCC 2 cut(s) 382, 731
ApeKI GCWGC 2 cut(s) 119, 2005
ApoI RAATTY 2 cut(s) 1028, 1691
Asp700I GAANNNNTTC 1 cut(s) 1370
AspLEI GCGC 1 cut(s) 1387
AspS9I GGNCC 3 cut(s) 209, 831, 1616
AsuC2I CCSGG 2 cut(s) 1269, 1270
AsuHPI GGTGA 6 cut(s) 77, 121, 442, 904, 1006, 1366
AvaI CYCGRG 1 cut(s) 1268
AvaII GGWCC 3 cut(s) 209, 831, 1616
BaeGI GKGCMC 1 cut(s) 904
BanI GGYRCC 1 cut(s) 838
BanII GRGCYC 2 cut(s) 891, 1196
BbsI GAAGAC 5 cut(s) 576, 1078, 1165, 1885, 2045
Bbv12I GWGCWC 1 cut(s) 1553
BbvI GCAGC 2 cut(s) 106, 2017
BccI CCATC 8 cut(s) 94, 220, 742, 809, 1160, 1648, 1763, 1837
BceAI ACGGC 2 cut(s) 659, 1882
BciT130I CCWGG 1 cut(s) 629
BclI TGATCA 1 cut(s) 1824
BcnI CCSGG 2 cut(s) 1269, 1270
BcoDI GTCTC 5 cut(s) 20, 536, 802, 1232, 1947
BfaI CTAG 2 cut(s) 320, 1940
BfmI CTRYAG 2 cut(s) 114, 970
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 3 cut(s) 120, 699, 2006
BlpI GCTNAGC 1 cut(s) 647
BlsI GCNGC 3 cut(s) 121, 700, 2007
Bme1390I CCNGG 3 cut(s) 629, 1269, 1270
Bme18I GGWCC 3 cut(s) 209, 831, 1616
BmeRI GACNNNNNGTC 1 cut(s) 1118
BmeT110I CYCGRG 1 cut(s) 1268
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 3 cut(s) 209, 831, 1616
BmiI GGNNCC 6 cut(s) 210, 459, 832, 840, 1617, 1946
BmrFI CCNGG 3 cut(s) 629, 1269, 1270
BmrI ACTGGG 2 cut(s) 251, 457
BmsI GCATC 5 cut(s) 918, 984, 1513, 1963, 1973
BmuI ACTGGG 2 cut(s) 251, 457
BpiI GAAGAC 5 cut(s) 576, 1078, 1165, 1885, 2045
BpmI CTGGAG 1 cut(s) 1230
Bpu1102I GCTNAGC 1 cut(s) 647
BpuMI CCSGG 2 cut(s) 1269, 1270
Bsa29I ATCGAT 2 cut(s) 1731, 1841
BsaBI GATNNNNATC 1 cut(s) 1451
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 7 cut(s) 123, 453, 834, 904, 1268, 1573, 1948
BsaWI WCCGGW 2 cut(s) 665, 1933
BsaXI ACNNNNNCTCC 4 cut(s) 782, 812, 1286, 1316
Bsc4I CCNNNNNNNGG 6 cut(s) 358, 559, 683, 1867, 1940, 2065
Bse1I ACTGG 5 cut(s) 20, 257, 463, 1009, 1247
Bse8I GATNNNNATC 1 cut(s) 1451
BseAI TCCGGA 1 cut(s) 1933
BseBI CCWGG 1 cut(s) 629
BseCI ATCGAT 2 cut(s) 1731, 1841
BseDI CCNNGG 7 cut(s) 123, 453, 834, 904, 1268, 1573, 1948
BseGI GGATG 6 cut(s) 624, 753, 1221, 1504, 1659, 2027
BseJI GATNNNNATC 1 cut(s) 1451
BseLI CCNNNNNNNGG 6 cut(s) 358, 559, 683, 1867, 1940, 2065
BseMII CTCAG 1 cut(s) 1245
BseNI ACTGG 5 cut(s) 20, 257, 463, 1009, 1247
BseRI GAGGAG 1 cut(s) 240
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 2 cut(s) 106, 2017
BsgI GTGCAG 1 cut(s) 2024
Bsh1236I CGCG 3 cut(s) 701, 1629, 1651
Bsh1285I CGRYCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 384, 733
BshNI GGYRCC 1 cut(s) 838
BshVI ATCGAT 2 cut(s) 1731, 1841
BsiEI CGRYCG 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 1553
BsiHKCI CYCGRG 1 cut(s) 1268
BsiSI CCGG 3 cut(s) 666, 1269, 1934
BsiWI CGTACG 1 cut(s) 443
BslFI GGGAC 6 cut(s) 222, 386, 844, 1612, 1629, 1923
BslI CCNNNNNNNGG 6 cut(s) 358, 559, 683, 1867, 1940, 2065
BsmAI GTCTC 5 cut(s) 20, 536, 802, 1232, 1947
BsmBI CGTCTC 1 cut(s) 1232
BsmFI GGGAC 6 cut(s) 222, 386, 844, 1612, 1629, 1923
BsmI GAATGC 5 cut(s) 945, 971, 1087, 1370, 1372
BsnI GGCC 2 cut(s) 384, 733
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 1268
Bsp1286I GDGCHC 4 cut(s) 891, 904, 1196, 1553
Bsp13I TCCGGA 1 cut(s) 1933
Bsp1407I TGTACA 2 cut(s) 1035, 1587
Bsp143I GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
Bsp1720I GCTNAGC 1 cut(s) 647
Bsp19I CCATGG 2 cut(s) 453, 834
BspACI CCGC 5 cut(s) 600, 699, 1377, 1627, 1651
BspANI GGCC 2 cut(s) 384, 733
BspCNI CTCAG 1 cut(s) 1244
BspDI ATCGAT 2 cut(s) 1731, 1841
BspEI TCCGGA 1 cut(s) 1933
BspFNI CGCG 3 cut(s) 701, 1629, 1651
BspHI TCATGA 3 cut(s) 816, 894, 978
BspLI GGNNCC 6 cut(s) 210, 459, 832, 840, 1617, 1946
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 1 cut(s) 1694
BspT107I GGYRCC 1 cut(s) 838
BspTNI GGTCTC 1 cut(s) 20
BsrGI TGTACA 2 cut(s) 1035, 1587
BsrI ACTGG 5 cut(s) 20, 257, 463, 1009, 1247
BssECI CCNNGG 7 cut(s) 123, 453, 834, 904, 1268, 1573, 1948
BssMI GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
BssT1I CCWWGG 4 cut(s) 123, 453, 834, 904
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 5 cut(s) 357, 448, 640, 1592, 1684
Bst6I CTCTTC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 1093
BstAUI TGTACA 2 cut(s) 1035, 1587
BstC8I GCNNGC 4 cut(s) 192, 305, 864, 1351
BstDEI CTNAG 5 cut(s) 220, 506, 647, 1047, 1231
BstDSI CCRYGG 2 cut(s) 453, 834
BstEII GGTNACC 2 cut(s) 127, 448
BstF5I GGATG 6 cut(s) 624, 753, 1221, 1504, 1659, 2027
BstFNI CGCG 3 cut(s) 701, 1629, 1651
BstHHI GCGC 1 cut(s) 1387
BstKTI GATC 7 cut(s) 47, 1309, 1605, 1702, 1731, 1827, 1841
BstMAI GTCTC 5 cut(s) 20, 536, 802, 1232, 1947
BstMBI GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
BstMCI CGRYCG 1 cut(s) 359
BstNI CCWGG 1 cut(s) 629
BstNSI RCATGY 5 cut(s) 307, 1129, 1787, 1805, 1960
BstPI GGTNACC 2 cut(s) 127, 448
BstSCI CCNGG 3 cut(s) 627, 1267, 1268
BstSFI CTRYAG 2 cut(s) 114, 970
BstSLI GKGCMC 1 cut(s) 904
BstUI CGCG 3 cut(s) 701, 1629, 1651
BstV1I GCAGC 2 cut(s) 106, 2017
BstV2I GAAGAC 5 cut(s) 576, 1078, 1165, 1885, 2045
Bsu15I ATCGAT 2 cut(s) 1731, 1841
BsuRI GGCC 2 cut(s) 384, 733
BsuTUI ATCGAT 2 cut(s) 1731, 1841
BtgI CCRYGG 2 cut(s) 453, 834
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 6 cut(s) 624, 753, 1221, 1504, 1659, 2027
BtsI GCAGTG 2 cut(s) 498, 1360
BtsIMutI CAGTG 6 cut(s) 498, 506, 1360, 1480, 1597, 1998
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 4 cut(s) 192, 305, 864, 1351
CciI TCATGA 3 cut(s) 816, 894, 978
CfoI GCGC 1 cut(s) 1387
Cfr13I GGNCC 3 cut(s) 209, 831, 1616
Cfr9I CCCGGG 1 cut(s) 1268
ClaI ATCGAT 2 cut(s) 1731, 1841
CseI GACGC 3 cut(s) 881, 1657, 1804
Csp6I GTAC 7 cut(s) 444, 641, 1036, 1333, 1578, 1588, 1799
CviQI GTAC 7 cut(s) 444, 641, 1036, 1333, 1578, 1588, 1799
DdeI CTNAG 5 cut(s) 220, 506, 647, 1047, 1231
DpnI GATC 7 cut(s) 46, 1308, 1604, 1701, 1730, 1826, 1840
DpnII GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
DraI TTTAAA 1 cut(s) 2053
DrdI GACNNNNNNGTC 1 cut(s) 923
DriI GACNNNNNGTC 1 cut(s) 1118
DseDI GACNNNNNNGTC 1 cut(s) 923
Eam1104I CTCTTC 1 cut(s) 143
Eam1105I GACNNNNNGTC 1 cut(s) 1118
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 4 cut(s) 123, 453, 834, 904
Eco147I AGGCCT 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 891, 1196
Eco31I GGTCTC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 2047
Eco47I GGWCC 3 cut(s) 209, 831, 1616
Eco57I CTGAAG 1 cut(s) 1309
Eco88I CYCGRG 1 cut(s) 1268
Eco91I GGTNACC 2 cut(s) 127, 448
EcoO109I RGGNCCY 1 cut(s) 1616
EcoO65I GGTNACC 2 cut(s) 127, 448
EcoRII CCWGG 1 cut(s) 627
EcoRV GATATC 1 cut(s) 2047
EcoT14I CCWWGG 4 cut(s) 123, 453, 834, 904
EcoT22I ATGCAT 1 cut(s) 1372
EcoT38I GRGCYC 2 cut(s) 891, 1196
ErhI CCWWGG 4 cut(s) 123, 453, 834, 904
Esp3I CGTCTC 1 cut(s) 1232
FaqI GGGAC 6 cut(s) 222, 386, 844, 1612, 1629, 1923
FbaI TGATCA 1 cut(s) 1824
FblI GTMKAC 3 cut(s) 1569, 1788, 1818
Fnu4HI GCNGC 3 cut(s) 120, 699, 2006
FokI GGATG 6 cut(s) 611, 760, 1208, 1491, 1666, 2034
FriOI GRGCYC 2 cut(s) 891, 1196
Fsp4HI GCNGC 3 cut(s) 120, 699, 2006
FspBI CTAG 2 cut(s) 320, 1940
GlaI GCGC 1 cut(s) 1386
GluI GCNGC 3 cut(s) 120, 699, 2006
GsuI CTGGAG 1 cut(s) 1230
HaeIII GGCC 2 cut(s) 384, 733
HapII CCGG 3 cut(s) 666, 1269, 1934
HgaI GACGC 3 cut(s) 881, 1657, 1804
HhaI GCGC 1 cut(s) 1387
Hin6I GCGC 1 cut(s) 1385
HinP1I GCGC 1 cut(s) 1385
HincII GTYRAC 2 cut(s) 1522, 1819
HindII GTYRAC 2 cut(s) 1522, 1819
HinfI GANTC 6 cut(s) 603, 1008, 1561, 1733, 1892, 2069
HpaII CCGG 3 cut(s) 666, 1269, 1934
HphI GGTGA 6 cut(s) 77, 121, 442, 904, 1006, 1366
Hpy166II GTNNAC 8 cut(s) 253, 636, 1062, 1216, 1522, 1570, 1789, 1819
Hpy188I TCNGA 8 cut(s) 164, 353, 562, 1112, 1306, 1328, 1738, 1952
Hpy188III TCNNGA 5 cut(s) 360, 817, 895, 979, 1934
Hpy8I GTNNAC 8 cut(s) 253, 636, 1062, 1216, 1522, 1570, 1789, 1819
Hpy99I CGWCG 5 cut(s) 357, 875, 1409, 1651, 1820
HpyAV CCTTC 4 cut(s) 852, 929, 1333, 2059
HpyCH4III ACNGT 5 cut(s) 357, 448, 640, 1592, 1684
HpyCH4IV ACGT 2 cut(s) 442, 519
HpyF3I CTNAG 5 cut(s) 220, 506, 647, 1047, 1231
HpySE526I ACGT 2 cut(s) 442, 519
HspAI GCGC 1 cut(s) 1385
Kpn2I TCCGGA 1 cut(s) 1933
Ksp22I TGATCA 1 cut(s) 1824
Kzo9I GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
LmnI GCTCC 5 cut(s) 555, 562, 1249, 1549, 1556
Lsp1109I GCAGC 2 cut(s) 106, 2017
LweI GCATC 5 cut(s) 918, 984, 1513, 1963, 1973
MaeI CTAG 2 cut(s) 320, 1940
MaeII ACGT 2 cut(s) 442, 519
MaeIII GTNAC 3 cut(s) 127, 448, 1684
MalI GATC 7 cut(s) 46, 1308, 1604, 1701, 1730, 1826, 1840
MboI GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
MfeI CAATTG 1 cut(s) 1380
MhlI GDGCHC 4 cut(s) 891, 904, 1196, 1553
MluCI AATT 8 cut(s) 143, 714, 1028, 1380, 1496, 1691, 1863, 1978
MlyI GAGTC 1 cut(s) 612
MmeI TCCRAC 2 cut(s) 376, 1205
Mph1103I ATGCAT 1 cut(s) 1372
MroI TCCGGA 1 cut(s) 1933
MroXI GAANNNNTTC 1 cut(s) 1370
MseI TTAA 7 cut(s) 261, 330, 689, 780, 1806, 2052, 2107
MslI CAYNNNNRTG 3 cut(s) 491, 983, 1887
MspI CCGG 3 cut(s) 666, 1269, 1934
MspR9I CCNGG 3 cut(s) 629, 1269, 1270
MunI CAATTG 1 cut(s) 1380
Mva1269I GAATGC 5 cut(s) 945, 971, 1087, 1370, 1372
MvaI CCWGG 1 cut(s) 629
MvnI CGCG 3 cut(s) 701, 1629, 1651
NciI CCSGG 2 cut(s) 1269, 1270
NcoI CCATGG 2 cut(s) 453, 834
NdeII GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
NlaIV GGNNCC 6 cut(s) 210, 459, 832, 840, 1617, 1946
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 2 cut(s) 127, 448
NsiI ATGCAT 1 cut(s) 1372
NspI RCATGY 5 cut(s) 307, 1129, 1787, 1805, 1960
PaeI GCATGC 1 cut(s) 307
PagI TCATGA 3 cut(s) 816, 894, 978
PceI AGGCCT 1 cut(s) 384
PciI ACATGT 2 cut(s) 1801, 1956
PctI GAATGC 5 cut(s) 945, 971, 1087, 1370, 1372
PdmI GAANNNNTTC 1 cut(s) 1370
PfeI GAWTC 5 cut(s) 1008, 1561, 1733, 1892, 2069
Pfl23II CGTACG 1 cut(s) 443
PkrI GCNGC 3 cut(s) 121, 700, 2007
PleI GAGTC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 611
PpuMI RGGWCCY 1 cut(s) 1616
PscI ACATGT 2 cut(s) 1801, 1956
PsiI TTATAA 1 cut(s) 1745
Psp5II RGGWCCY 1 cut(s) 1616
Psp6I CCWGG 1 cut(s) 627
PspEI GGTNACC 2 cut(s) 127, 448
PspGI CCWGG 1 cut(s) 627
PspLI CGTACG 1 cut(s) 443
PspN4I GGNNCC 6 cut(s) 210, 459, 832, 840, 1617, 1946
PspPI GGNCC 3 cut(s) 209, 831, 1616
PspPPI RGGWCCY 1 cut(s) 1616
RsaI GTAC 7 cut(s) 445, 642, 1037, 1334, 1579, 1589, 1800
RsaNI GTAC 7 cut(s) 444, 641, 1036, 1333, 1578, 1588, 1799
RseI CAYNNNNRTG 3 cut(s) 491, 983, 1887
SalI GTCGAC 1 cut(s) 1817
SaqAI TTAA 7 cut(s) 261, 330, 689, 780, 1806, 2052, 2107
SatI GCNGC 3 cut(s) 120, 699, 2006
Sau3AI GATC 7 cut(s) 44, 1306, 1602, 1699, 1728, 1824, 1838
Sau96I GGNCC 3 cut(s) 209, 831, 1616
SchI GAGTC 1 cut(s) 612
ScrFI CCNGG 3 cut(s) 629, 1269, 1270
SduI GDGCHC 4 cut(s) 891, 904, 1196, 1553
SfaNI GCATC 5 cut(s) 918, 984, 1513, 1963, 1973
SfcI CTRYAG 2 cut(s) 114, 970
SinI GGWCC 3 cut(s) 209, 831, 1616
SmaI CCCGGG 1 cut(s) 1270
SmiMI CAYNNNNRTG 3 cut(s) 491, 983, 1887
SphI GCATGC 1 cut(s) 307
Sse9I AATT 8 cut(s) 143, 714, 1028, 1380, 1496, 1691, 1863, 1978
SseBI AGGCCT 1 cut(s) 384
SsiI CCGC 5 cut(s) 600, 699, 1377, 1627, 1651
SspI AATATT 1 cut(s) 1531
SspMI CTAG 2 cut(s) 320, 1940
StuI AGGCCT 1 cut(s) 384
StyD4I CCNGG 3 cut(s) 627, 1267, 1268
StyI CCWWGG 4 cut(s) 123, 453, 834, 904
TaaI ACNGT 5 cut(s) 357, 448, 640, 1592, 1684
TaiI ACGT 2 cut(s) 445, 522
TaqI TCGA 9 cut(s) 47, 590, 870, 1237, 1731, 1818, 1841, 1857, 2101
TaqII GACCGA 2 cut(s) 975, 1587
TasI AATT 8 cut(s) 143, 714, 1028, 1380, 1496, 1691, 1863, 1978
TatI WGTACW 2 cut(s) 1035, 1587
TauI GCSGC 1 cut(s) 701
TfiI GAWTC 5 cut(s) 1008, 1561, 1733, 1892, 2069
Tru1I TTAA 7 cut(s) 261, 330, 689, 780, 1806, 2052, 2107
Tru9I TTAA 7 cut(s) 261, 330, 689, 780, 1806, 2052, 2107
TscAI CASTG 6 cut(s) 498, 506, 1360, 1480, 1597, 1998
TseFI GTSAC 2 cut(s) 127, 448
TseI GCWGC 2 cut(s) 119, 2005
Tsp45I GTSAC 2 cut(s) 127, 448
TspDTI ATGAA 9 cut(s) 17, 136, 671, 712, 796, 870, 1379, 1763, 1894
TspGWI ACGGA 3 cut(s) 1214, 1536, 1543
TspMI CCCGGG 1 cut(s) 1268
TspRI CASTG 6 cut(s) 498, 506, 1360, 1480, 1597, 1998
VpaK11BI GGWCC 3 cut(s) 209, 831, 1616
XapI RAATTY 2 cut(s) 1028, 1691
XceI RCATGY 5 cut(s) 307, 1129, 1787, 1805, 1960
XcmI CCANNNNNNNNNTGG 2 cut(s) 741, 808
XmaI CCCGGG 1 cut(s) 1268
XmiI GTMKAC 3 cut(s) 1569, 1788, 1818
XmnI GAANNNNTTC 1 cut(s) 1370
XspI CTAG 2 cut(s) 320, 1940
Zsp2I ATGCAT 1 cut(s) 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.