Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0014704

Physical Location & Seq

Genomic Coordinates Reverse (-)

5016786 .. 5020001

Gene Structure 3216 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1059100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGCAAACCCTACGTTTCAAGCCTGAGCTTCTGGACGATTCGAACGCAATGGTTAAGGCAAATGCATTGAGTAATCAGACCTGGGTATGTCAGGTTCGGAAATTATCAAGACCAAAAGGCTATTTTTTCGGTGATAGCCCTTTAAACTTCGATACTCCCCTTCTTTACGTACAGCTTTCCATATCTGCCTTACTCGCCGCTGTCCTCCAATTCATTCTCAACCCTCTTGGCCAGACTGCTTTCATACCACAAATGCTCGCAGGTATTATACTGGGACCATCAGTCATAGGATCAGGCAATGCCTTTGCAGATACAGTTTTCCCACTGAAAAGCTTTTACATCAGCCAAACCTATGCGTTATTTGGTGTCATGCTGTTTCTCTTCCTAGTTGGAGTTAAAATGGACATAAGTTTGGTGAAACAATCAGGAAGGAAAGCCGTGGTCATAGGCCTCTCGTCTTTCTTCGTCCCTTTGATCCTCAACATCAGCTTCGCTTTTATCCTACGAAAGACAGTCGCGATGGAAACAAATCTACGAAAGTCGATCATCATCATCGCGGTGTTTCAATGCCTAAGCTCGTTCCACGTCATCGCCTGCCTCTTGGCCGATTTGAATCTCCTCAACTCGGAGATTGGGAGATTGGCTGTGTCTTCATCAACAGTCAGTGGGGTGCTATCTTTGATTTGGGTCAGTTTGGCCTTCACAATAAGGCAAAGCAACATTTCAAAAAAGGATCTTAGTCTACCCTTTATGGGGTTGAGCTTGTTTTGCATGTTCATCCTCATAGTCTACATTTTGAGGCCTATAATGTTATGGATAGTTGCACAAATTACAGATAAAGGAAGATCCATGAAAGATAGCTACATTTTTTCAGTATTTATCATGATAATGGTGTGTTCTTTTCTTGGTGAGGTTGTGGGCCAGCACTTTATAATGGGGCCCATGATTTTAGGGTTGGCAGTGCCAGATGGGCCACCATTGGGATCAGCTTTGGTTGAGAAACTTGACTCCTACGTCTCATCGATTCTGTTGCCGAGCTACTTTGTGCTCACTGGTGCAAGGATTAATTTCTCCATGATAAGGATGAAGACTGTTTGGATTGTGGAGCTATTGGTTATGAGTAGCTTTTGTGGTAAACTTGTAGGAACTGTGGTGCCTTCTCTCTATTTCAAAATGTCTCCTGCCGATTCACTTTCACTAGGGCTTATCATGAGTGCTCAAGGCATCCTTGACGTTATAAATTTGAACCATGCATTGTTACTCTTCCTGATTGACGAAGAAGCCTATAGCGTTATGGCTCTGTCAGCACTCGTCTTAACTGCAATAATCACCCCCATAGTGAAATATCTGTACAAACCATCCAAGAGATACATGTCCACCAAAAGGAGGACAATTGAGCATGCCTTTCACAATGCCGAGCTTCGAATTCTTGCGTGCATATACCAAGAAGAGAGCACCCCTTCCATCATCAGCCTTCTTGAAGTCTCCAACCCAACCCCCAAAACCCCCATATGCTTTTATGTTGTCCACCTCGTGAGACTATCCGGCAGAACCGCCCCAATCCTCACAACACATCGACTCGAAAAAAGGAGTGCTAGTACATTTCATTCCGGTAACTCAGATCGGATAGTCAATGCCTTCAGATTATATGAAGAACATAGCTCTGGTGGCCTCATAATGAATGCATTGACAGCCATTGCACCCTACGCCACAATGCATGATGATGTTTGCACATTGGCATTGGAAAAAAGGACTTCCATGGTCCTAATCCCATTCCACAAGCAATTCAATTCGCTAAGTTGCACAGAGGAATTATCCAAACCTATTAGGTCCGTGAATCTAAACATTCTTCGAAATTCCCCATGCTCAGTTGGGATTCTCCTCGATCGTGGTACCTTGAACACCAATACATCTCTATCATGCAAAAATTTGTACAATATTGGGATTATCTTTGTAGAAGGACCTGATGACCGTGAGGCATTGGCATATGCAATGCGCATGGCGGAGCATCCTTTTGTCAGCCTCACGGTCGTTAGATTGGCTGACAATAGGAAATGCAGTGTGCATAAAAAACATGATTTGGAGCTACTCAGCAAGTGTAAGATAGAAATTGCAGGGAAAAAACAACATGTTTACAAGGAGGAACGTGTTAAAGATAGCGTGGACATGATCAATGTGATTAGGACCATAGAAAATTTTTATGACCTAATTTTGGTGGGGAGACGCCATGATAGTGATTCACCATTGTTCATGGGACTCACAGAATGGAATGAGTTTCCGGAACTTGGGTTCATTGGAGATATGTTGGCATCTTCAGATTCCAATTGTGAGGTCTCGGTATTGGTGGTGCAGCAACAAATGGTTGGAAATGATCAACAGATGGTAGACGACAACAGCTTTAATAAGTCCTATGGAATCCCATCATTTTCTGTCGTGGATATTCCTCGTGATGATAGAGTGCACCCGCTTCCTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0031410 GO:0031982 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

826

Amino Acids

91.78

Weight (kDa)

8.55

Isoelectric Point (pI)

44.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	63 - 448	1.3e-35	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	505 - 643	1.5e-17	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	647 - 786	3.5e-16	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014704)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g39830 FvH4_3g39830
malus_domestica	MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica	Prupe.6G047700_v2.0.a1
pyrus_communis	pycom11g04890
rosa_chinensis	RchiOBHm_Chr5g0071771
rosa_laevigata	RLG00000036244
rosa_multiflora	Rmu_sc0001113.1_g000001
rosa_roxburghii	Rroxscaffold_1G00009450
rosa_rugosa	Rorug05G0414400 Rorug05G0414500
rosa_samantha	Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana	Rw5G043720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	932, 1238
AasI	GACNNNNNNGTC	1 cut(s)	1013
Acc16I	TGCGCA	1 cut(s)	1997
Acc36I	ACCTGC	1 cut(s)	251
Acc65I	GGTACC	1 cut(s)	1892
AccB1I	GGYRCC	2 cut(s)	1153, 1892
AccI	GTMKAC	3 cut(s)	742, 789, 2385
AccII	CGCG	2 cut(s)	518, 557
AccIII	TCCGGA	1 cut(s)	2278
AciI	CCGC	5 cut(s)	198, 557, 1554, 2003, 2465
AclWI	GGATC	5 cut(s)	298, 469, 741, 840, 991
AcoI	YGGCCR	2 cut(s)	229, 603
AcsI	RAATTY	5 cut(s)	1240, 1425, 1855, 1927, 2194
AcuI	CTGAAG	2 cut(s)	1624, 2298
AcyI	GRCGYC	1 cut(s)	2224
AdeI	CACNNNGTG	1 cut(s)	1534
AfaI	GTAC	5 cut(s)	171, 1352, 1600, 1894, 1934
AfiI	CCNNNNNNNGG	9 cut(s)	625, 752, 753, 980, 1080, 1383, 1386, 2212, 2285
AflIII	ACRYGT	3 cut(s)	1371, 2128, 2146
AgsI	TTSAA	9 cut(s)	19, 566, 613, 726, 1171, 1246, 1481, 1789, 1900
AhdI	GACNNNNNGTC	1 cut(s)	281
AjiI	CACGTC	1 cut(s)	586
AjnI	CCWGG	1 cut(s)	80
AloI	GAACNNNNNNTCC	2 cut(s)	2273, 2305
Alw21I	GWGCWC	4 cut(s)	1050, 1219, 1457, 2463
Alw26I	GTCTC	6 cut(s)	1021, 1182, 1489, 1531, 2215, 2338
Alw44I	GTGCAC	1 cut(s)	2459
AlwI	GGATC	5 cut(s)	298, 469, 741, 840, 991
Aor13HI	TCCGGA	1 cut(s)	2278
AoxI	GGCC	9 cut(s)	229, 448, 603, 696, 800, 919, 938, 971, 1669
ApaI	GGGCCC	1 cut(s)	942
ApaLI	GTGCAC	1 cut(s)	2459
ApeKI	GCWGC	1 cut(s)	2350
ApoI	RAATTY	5 cut(s)	1240, 1425, 1855, 1927, 2194
AseI	ATTAAT	1 cut(s)	1065
Asp718I	GGTACC	1 cut(s)	1892
AspLEI	GCGC	1 cut(s)	1998
AspS9I	GGNCC	9 cut(s)	275, 919, 938, 939, 971, 1762, 1830, 1961, 2184
AsuHPI	GGTGA	5 cut(s)	143, 427, 920, 1321, 2232
AsuII	TTCGAA	3 cut(s)	41, 1423, 1852
AvaII	GGWCC	5 cut(s)	275, 1762, 1830, 1961, 2184
BaeGI	GKGCMC	2 cut(s)	942, 2463
BalI	TGGCCA	1 cut(s)	231
BanI	GGYRCC	2 cut(s)	1153, 1892
BanII	GRGCYC	1 cut(s)	942
BauI	CACGAG	2 cut(s)	1532, 2445
BbsI	GAAGAC	2 cut(s)	642, 1094
Bbv12I	GWGCWC	4 cut(s)	1050, 1219, 1457, 2463
BbvI	GCAGC	1 cut(s)	2362
BccI	CCATC	7 cut(s)	286, 514, 962, 1366, 1472, 2374, 2428
BceAI	ACGGC	1 cut(s)	422
BcgI	CGANNNNNNTGC	2 cut(s)	1012, 1046
BciT130I	CCWGG	1 cut(s)	82
BclI	TGATCA	2 cut(s)	2169, 2371
BcoDI	GTCTC	6 cut(s)	1021, 1182, 1489, 1531, 2215, 2338
BfaI	CTAG	3 cut(s)	386, 1199, 1596
BfmI	CTRYAG	1 cut(s)	1285
BfuAI	ACCTGC	1 cut(s)	251
BglI	GCCNNNNNGGC	1 cut(s)	970
BisI	GCNGC	2 cut(s)	198, 2351
BlsI	GCNGC	2 cut(s)	199, 2352
Bme1390I	CCNGG	1 cut(s)	82
Bme18I	GGWCC	5 cut(s)	275, 1762, 1830, 1961, 2184
BmeRI	GACNNNNNGTC	1 cut(s)	281
BmgBI	CACGTC	1 cut(s)	586
BmgT120I	GGNCC	9 cut(s)	275, 919, 938, 939, 971, 1762, 1830, 1961, 2184
BmiI	GGNNCC	5 cut(s)	276, 939, 940, 1155, 1894
BmrFI	CCNGG	1 cut(s)	82
BmrI	ACTGGG	1 cut(s)	281
BmsI	GCATC	3 cut(s)	1233, 2017, 2318
BmuI	ACTGGG	1 cut(s)	281
BpiI	GAAGAC	2 cut(s)	642, 1094
Bpu10I	CCTNAGC	2 cut(s)	24, 572
Bpu14I	TTCGAA	3 cut(s)	41, 1423, 1852
BpuEI	CTTGAG	1 cut(s)	1203
Bsa29I	ATCGAT	1 cut(s)	1022
BsaAI	YACGTR	1 cut(s)	169
BsaBI	GATNNNNATC	2 cut(s)	548, 612
BsaHI	GRCGYC	1 cut(s)	2224
BsaI	GGTCTC	1 cut(s)	2338
BsaJI	CCNNGG	3 cut(s)	81, 438, 1758
BsaWI	WCCGGW	2 cut(s)	1610, 2278
BsaXI	ACNNNNNCTCC	2 cut(s)	1582, 1612
Bsc4I	CCNNNNNNNGG	9 cut(s)	625, 752, 753, 980, 1080, 1383, 1386, 2212, 2285
Bse1I	ACTGG	2 cut(s)	276, 1057
Bse3DI	GCAATG	4 cut(s)	54, 304, 1695, 1998
Bse8I	GATNNNNATC	2 cut(s)	548, 612
BseAI	TCCGGA	1 cut(s)	2278
BseBI	CCWGG	1 cut(s)	82
BseCI	ATCGAT	1 cut(s)	1022
BseDI	CCNNGG	3 cut(s)	81, 438, 1758
BseGI	GGATG	5 cut(s)	777, 1089, 1224, 1358, 2008
BseJI	GATNNNNATC	2 cut(s)	548, 612
BseLI	CCNNNNNNNGG	9 cut(s)	625, 752, 753, 980, 1080, 1383, 1386, 2212, 2285
BseMI	GCAATG	4 cut(s)	54, 304, 1695, 1998
BseMII	CTCAG	4 cut(s)	15, 1632, 1881, 2104
BseNI	ACTGG	2 cut(s)	276, 1057
BseRI	GAGGAG	2 cut(s)	608, 1871
BseSI	GKGCMC	2 cut(s)	942, 2463
BseXI	GCAGC	1 cut(s)	2362
BsgI	GTGCAG	1 cut(s)	2369
Bsh1236I	CGCG	2 cut(s)	518, 557
Bsh1285I	CGRYCG	2 cut(s)	1888, 2031
BshFI	GGCC	9 cut(s)	231, 450, 605, 698, 802, 921, 940, 973, 1671
BshNI	GGYRCC	2 cut(s)	1153, 1892
BshVI	ATCGAT	1 cut(s)	1022
BsiEI	CGRYCG	2 cut(s)	1888, 2031
BsiHKAI	GWGCWC	4 cut(s)	1050, 1219, 1457, 2463
BsiSI	CCGG	3 cut(s)	1545, 1611, 2279
BslFI	GGGAC	3 cut(s)	288, 452, 2268
BslI	CCNNNNNNNGG	9 cut(s)	625, 752, 753, 980, 1080, 1383, 1386, 2212, 2285
BsmAI	GTCTC	6 cut(s)	1021, 1182, 1489, 1531, 2215, 2338
BsmBI	CGTCTC	2 cut(s)	1021, 2215
BsmFI	GGGAC	3 cut(s)	288, 452, 2268
BsmI	GAATGC	1 cut(s)	1687
BsnI	GGCC	9 cut(s)	231, 450, 605, 698, 802, 921, 940, 973, 1671
Bso31I	GGTCTC	1 cut(s)	2338
Bsp119I	TTCGAA	3 cut(s)	41, 1423, 1852
Bsp120I	GGGCCC	1 cut(s)	938
Bsp1286I	GDGCHC	5 cut(s)	942, 1050, 1219, 1457, 2463
Bsp13I	TCCGGA	1 cut(s)	2278
Bsp1407I	TGTACA	2 cut(s)	1350, 1932
Bsp19I	CCATGG	1 cut(s)	1758
Bsp68I	TCGCGA	1 cut(s)	518
BspACI	CCGC	5 cut(s)	198, 557, 1554, 2003, 2465
BspANI	GGCC	9 cut(s)	231, 450, 605, 698, 802, 921, 940, 973, 1671
BspCNI	CTCAG	4 cut(s)	16, 1631, 1880, 2103
BspDI	ATCGAT	1 cut(s)	1022
BspEI	TCCGGA	1 cut(s)	2278
BspFNI	CGCG	2 cut(s)	518, 557
BspHI	TCATGA	2 cut(s)	882, 1209
BspLI	GGNNCC	5 cut(s)	276, 939, 940, 1155, 1894
BspMI	ACCTGC	1 cut(s)	251
BspPI	GGATC	5 cut(s)	298, 469, 741, 840, 991
BspT104I	TTCGAA	3 cut(s)	41, 1423, 1852
BspT107I	GGYRCC	2 cut(s)	1153, 1892
BspTNI	GGTCTC	1 cut(s)	2338
BsrDI	GCAATG	4 cut(s)	54, 304, 1695, 1998
BsrGI	TGTACA	2 cut(s)	1350, 1932
BsrI	ACTGG	2 cut(s)	276, 1057
BssECI	CCNNGG	3 cut(s)	81, 438, 1758
BssNI	GRCGYC	1 cut(s)	2224
BssSI	CACGAG	2 cut(s)	1532, 2445
BssT1I	CCWWGG	1 cut(s)	1758
Bst2BI	CACGAG	2 cut(s)	1532, 2445
Bst2UI	CCWGG	1 cut(s)	82
Bst4CI	ACNGT	7 cut(s)	316, 514, 661, 1093, 1150, 1973, 2029
Bst6I	CTCTTC	3 cut(s)	386, 1268, 1443
BstACI	GRCGYC	1 cut(s)	2224
BstAUI	TGTACA	2 cut(s)	1350, 1932
BstBAI	YACGTR	1 cut(s)	169
BstBI	TTCGAA	3 cut(s)	41, 1423, 1852
BstC8I	GCNNGC	5 cut(s)	258, 595, 923, 1401, 1435
BstDEI	CTNAG	7 cut(s)	24, 572, 737, 1618, 1796, 1867, 2090
BstDSI	CCRYGG	2 cut(s)	438, 1758
BstF5I	GGATG	5 cut(s)	777, 1089, 1224, 1358, 2008
BstFNI	CGCG	2 cut(s)	518, 557
BstHHI	GCGC	1 cut(s)	1998
BstMAI	GTCTC	6 cut(s)	1021, 1182, 1489, 1531, 2215, 2338
BstMCI	CGRYCG	2 cut(s)	1888, 2031
BstMWI	GCNNNNNNNGC	7 cut(s)	194, 768, 970, 1304, 1668, 1691, 1706
BstNI	CCWGG	1 cut(s)	82
BstNSI	RCATGY	4 cut(s)	775, 1375, 1403, 2132
BstSCI	CCNGG	1 cut(s)	80
BstSFI	CTRYAG	1 cut(s)	1285
BstSLI	GKGCMC	2 cut(s)	942, 2463
BstSNI	TACGTA	1 cut(s)	169
BstUI	CGCG	2 cut(s)	518, 557
BstV1I	GCAGC	1 cut(s)	2362
BstV2I	GAAGAC	2 cut(s)	642, 1094
BstX2I	RGATCY	2 cut(s)	733, 845
BstYI	RGATCY	2 cut(s)	733, 845
Bsu15I	ATCGAT	1 cut(s)	1022
BsuRI	GGCC	9 cut(s)	231, 450, 605, 698, 802, 921, 940, 973, 1671
BsuTUI	ATCGAT	1 cut(s)	1022
BtgI	CCRYGG	2 cut(s)	438, 1758
BtgZI	GCGATG	3 cut(s)	533, 538, 574
BtrI	CACGTC	1 cut(s)	586
BtsCI	GGATG	5 cut(s)	777, 1089, 1224, 1358, 2008
BtsI	GCAGTG	2 cut(s)	966, 2065
BtsIMutI	CAGTG	5 cut(s)	323, 670, 966, 1050, 2065
BtuMI	TCGCGA	1 cut(s)	518
BveI	ACCTGC	1 cut(s)	251
Cac8I	GCNNGC	5 cut(s)	258, 595, 923, 1401, 1435
CciI	TCATGA	2 cut(s)	882, 1209
CfoI	GCGC	1 cut(s)	1998
Cfr13I	GGNCC	9 cut(s)	275, 919, 938, 939, 971, 1762, 1830, 1961, 2184
ClaI	ATCGAT	1 cut(s)	1022
CseI	GACGC	1 cut(s)	2232
Csp6I	GTAC	5 cut(s)	170, 1351, 1599, 1893, 1933
CviQI	GTAC	5 cut(s)	170, 1351, 1599, 1893, 1933
DdeI	CTNAG	7 cut(s)	24, 572, 737, 1618, 1796, 1867, 2090
DraI	TTTAAA	1 cut(s)	144
DraIII	CACNNNGTG	1 cut(s)	1534
DrdI	GACNNNNNNGTC	1 cut(s)	1013
DriI	GACNNNNNGTC	1 cut(s)	281
DseDI	GACNNNNNNGTC	1 cut(s)	1013
EaeI	YGGCCR	2 cut(s)	229, 603
Eam1104I	CTCTTC	3 cut(s)	386, 1268, 1443
Eam1105I	GACNNNNNGTC	1 cut(s)	281
EarI	CTCTTC	3 cut(s)	386, 1268, 1443
EciI	GGCGGA	1 cut(s)	2018
Eco105I	TACGTA	1 cut(s)	169
Eco130I	CCWWGG	1 cut(s)	1758
Eco147I	AGGCCT	2 cut(s)	450, 802
Eco24I	GRGCYC	1 cut(s)	942
Eco31I	GGTCTC	1 cut(s)	2338
Eco47I	GGWCC	5 cut(s)	275, 1762, 1830, 1961, 2184
Eco57I	CTGAAG	2 cut(s)	1624, 2298
EcoO109I	RGGNCCY	2 cut(s)	938, 1961
EcoRI	GAATTC	1 cut(s)	1425
EcoRII	CCWGG	1 cut(s)	80
EcoT14I	CCWWGG	1 cut(s)	1758
EcoT22I	ATGCAT	4 cut(s)	67, 1255, 1687, 1719
EcoT38I	GRGCYC	1 cut(s)	942
ErhI	CCWWGG	1 cut(s)	1758
Esp3I	CGTCTC	2 cut(s)	1021, 2215
FalI	AAGNNNNNCTT	2 cut(s)	1212, 1244
FaqI	GGGAC	3 cut(s)	288, 452, 2268
FauI	CCCGC	1 cut(s)	2472
FauNDI	CATATG	2 cut(s)	1511, 1987
FbaI	TGATCA	2 cut(s)	2169, 2371
FblI	GTMKAC	3 cut(s)	742, 789, 2385
Fnu4HI	GCNGC	2 cut(s)	198, 2351
FokI	GGATG	5 cut(s)	764, 1096, 1211, 1345, 1995
FriOI	GRGCYC	1 cut(s)	942
Fsp4HI	GCNGC	2 cut(s)	198, 2351
FspAI	RTGCGCAY	1 cut(s)	1997
FspBI	CTAG	3 cut(s)	386, 1199, 1596
FspI	TGCGCA	1 cut(s)	1997
GlaI	GCGC	1 cut(s)	1997
GluI	GCNGC	2 cut(s)	198, 2351
HaeIII	GGCC	9 cut(s)	231, 450, 605, 698, 802, 921, 940, 973, 1671
HapII	CCGG	3 cut(s)	1545, 1611, 2279
HgaI	GACGC	1 cut(s)	2232
HhaI	GCGC	1 cut(s)	1998
Hin1I	GRCGYC	1 cut(s)	2224
Hin6I	GCGC	1 cut(s)	1996
HinP1I	GCGC	1 cut(s)	1996
HindIII	AAGCTT	1 cut(s)	331
HpaII	CCGG	3 cut(s)	1545, 1611, 2279
HphI	GGTGA	5 cut(s)	143, 427, 920, 1321, 2232
Hpy166II	GTNNAC	9 cut(s)	743, 790, 1136, 1377, 1528, 2134, 2164, 2386, 2461
Hpy188I	TCNGA	7 cut(s)	78, 99, 628, 1621, 1626, 1643, 2317
Hpy8I	GTNNAC	9 cut(s)	743, 790, 1136, 1377, 1528, 2134, 2164, 2386, 2461
HpyAV	CCTTC	8 cut(s)	170, 423, 709, 1167, 1470, 1484, 1648, 1952
HpyCH4III	ACNGT	7 cut(s)	316, 514, 661, 1093, 1150, 1973, 2029
HpyCH4IV	ACGT	6 cut(s)	13, 168, 585, 1014, 1233, 2146
HpyF10VI	GCNNNNNNNGC	7 cut(s)	194, 768, 970, 1304, 1668, 1691, 1706
HpyF3I	CTNAG	7 cut(s)	24, 572, 737, 1618, 1796, 1867, 2090
HpySE526I	ACGT	6 cut(s)	13, 168, 585, 1014, 1233, 2146
Hsp92I	GRCGYC	1 cut(s)	2224
HspAI	GCGC	1 cut(s)	1996
Kpn2I	TCCGGA	1 cut(s)	2278
KpnI	GGTACC	1 cut(s)	1896
Ksp22I	TGATCA	2 cut(s)	2169, 2371
LmnI	GCTCC	3 cut(s)	1105, 2005, 2083
Lsp1109I	GCAGC	1 cut(s)	2362
LweI	GCATC	3 cut(s)	1233, 2017, 2318
MaeI	CTAG	3 cut(s)	386, 1199, 1596
MaeII	ACGT	6 cut(s)	13, 168, 585, 1014, 1233, 2146
MaeIII	GTNAC	2 cut(s)	1257, 1613
MfeI	CAATTG	2 cut(s)	1392, 2323
MflI	RGATCY	2 cut(s)	733, 845
MhlI	GDGCHC	5 cut(s)	942, 1050, 1219, 1457, 2463
MlsI	TGGCCA	1 cut(s)	231
MluNI	TGGCCA	1 cut(s)	231
MlyI	GAGTC	3 cut(s)	1001, 1572, 2250
MmeI	TCCRAC	3 cut(s)	370, 1512, 2344
Mox20I	TGGCCA	1 cut(s)	231
Mph1103I	ATGCAT	4 cut(s)	67, 1255, 1687, 1719
MroI	TCCGGA	1 cut(s)	2278
MscI	TGGCCA	1 cut(s)	231
MseI	TTAA	7 cut(s)	54, 143, 396, 1065, 1316, 2151, 2400
MslI	CAYNNNNRTG	4 cut(s)	557, 887, 1722, 2232
Msp20I	TGGCCA	1 cut(s)	231
MspA1I	CMGCKG	1 cut(s)	200
MspI	CCGG	3 cut(s)	1545, 1611, 2279
MspR9I	CCNGG	1 cut(s)	82
MunI	CAATTG	2 cut(s)	1392, 2323
Mva1269I	GAATGC	1 cut(s)	1687
MvaI	CCWGG	1 cut(s)	82
MvnI	CGCG	2 cut(s)	518, 557
MwoI	GCNNNNNNNGC	7 cut(s)	194, 768, 970, 1304, 1668, 1691, 1706
NcoI	CCATGG	1 cut(s)	1758
NdeI	CATATG	2 cut(s)	1511, 1987
NlaIV	GGNNCC	5 cut(s)	276, 939, 940, 1155, 1894
NmeAIII	GCCGAG	2 cut(s)	1059, 1441
NruI	TCGCGA	1 cut(s)	518
NsbI	TGCGCA	1 cut(s)	1997
NsiI	ATGCAT	4 cut(s)	67, 1255, 1687, 1719
NspI	RCATGY	4 cut(s)	775, 1375, 1403, 2132
NspV	TTCGAA	3 cut(s)	41, 1423, 1852
PaeI	GCATGC	1 cut(s)	1403
PagI	TCATGA	2 cut(s)	882, 1209
PceI	AGGCCT	2 cut(s)	450, 802
PciI	ACATGT	2 cut(s)	1371, 2128
PctI	GAATGC	1 cut(s)	1687
PfeI	GAWTC	9 cut(s)	38, 613, 1024, 1187, 1837, 1876, 2237, 2318, 2415
PkrI	GCNGC	2 cut(s)	199, 2352
Ple19I	CGATCG	1 cut(s)	1888
PleI	GAGTC	3 cut(s)	1001, 1572, 2250
PpsI	GAGTC	3 cut(s)	1001, 1572, 2250
Ppu21I	YACGTR	1 cut(s)	169
PpuMI	RGGWCCY	1 cut(s)	1961
PscI	ACATGT	2 cut(s)	1371, 2128
PshBI	ATTAAT	1 cut(s)	1065
PsiI	TTATAA	2 cut(s)	932, 1238
Psp5II	RGGWCCY	1 cut(s)	1961
Psp6I	CCWGG	1 cut(s)	80
PspGI	CCWGG	1 cut(s)	80
PspN4I	GGNNCC	5 cut(s)	276, 939, 940, 1155, 1894
PspOMI	GGGCCC	1 cut(s)	938
PspPI	GGNCC	9 cut(s)	275, 919, 938, 939, 971, 1762, 1830, 1961, 2184
PspPPI	RGGWCCY	1 cut(s)	1961
PsrI	GAACNNNNNNTAC	2 cut(s)	78, 110
PsuI	RGATCY	2 cut(s)	733, 845
PvuI	CGATCG	1 cut(s)	1888
RruI	TCGCGA	1 cut(s)	518
RsaI	GTAC	5 cut(s)	171, 1352, 1600, 1894, 1934
RsaNI	GTAC	5 cut(s)	170, 1351, 1599, 1893, 1933
RseI	CAYNNNNRTG	4 cut(s)	557, 887, 1722, 2232
SaqAI	TTAA	7 cut(s)	54, 143, 396, 1065, 1316, 2151, 2400
SatI	GCNGC	2 cut(s)	198, 2351
Sau96I	GGNCC	9 cut(s)	275, 919, 938, 939, 971, 1762, 1830, 1961, 2184
SchI	GAGTC	3 cut(s)	1001, 1572, 2250
ScrFI	CCNGG	1 cut(s)	82
SduI	GDGCHC	5 cut(s)	942, 1050, 1219, 1457, 2463
SfaNI	GCATC	3 cut(s)	1233, 2017, 2318
SfcI	CTRYAG	1 cut(s)	1285
SfuI	TTCGAA	3 cut(s)	41, 1423, 1852
SinI	GGWCC	5 cut(s)	275, 1762, 1830, 1961, 2184
SmiMI	CAYNNNNRTG	4 cut(s)	557, 887, 1722, 2232
SmlI	CTYRAG	1 cut(s)	1218
SmoI	CTYRAG	1 cut(s)	1218
SnaBI	TACGTA	1 cut(s)	169
SphI	GCATGC	1 cut(s)	1403
SseBI	AGGCCT	2 cut(s)	450, 802
SsiI	CCGC	5 cut(s)	198, 557, 1554, 2003, 2465
SspI	AATATT	1 cut(s)	1939
SspMI	CTAG	3 cut(s)	386, 1199, 1596
StuI	AGGCCT	2 cut(s)	450, 802
StyD4I	CCNGG	1 cut(s)	80
StyI	CCWWGG	1 cut(s)	1758
TaaI	ACNGT	7 cut(s)	316, 514, 661, 1093, 1150, 1973, 2029
TaiI	ACGT	6 cut(s)	16, 171, 588, 1017, 1236, 2149
TaqI	TCGA	9 cut(s)	41, 150, 542, 1022, 1423, 1576, 1581, 1852, 1884
TatI	WGTACW	3 cut(s)	1350, 1598, 1932
TauI	GCSGC	1 cut(s)	200
TfiI	GAWTC	9 cut(s)	38, 613, 1024, 1187, 1837, 1876, 2237, 2318, 2415
Tru1I	TTAA	7 cut(s)	54, 143, 396, 1065, 1316, 2151, 2400
Tru9I	TTAA	7 cut(s)	54, 143, 396, 1065, 1316, 2151, 2400
TscAI	CASTG	5 cut(s)	330, 670, 966, 1057, 2065
TseI	GCWGC	1 cut(s)	2350
TspGWI	ACGGA	1 cut(s)	1822
TspRI	CASTG	5 cut(s)	330, 670, 966, 1057, 2065
VneI	GTGCAC	1 cut(s)	2459
VpaK11BI	GGWCC	5 cut(s)	275, 1762, 1830, 1961, 2184
VspI	ATTAAT	1 cut(s)	1065
XapI	RAATTY	5 cut(s)	1240, 1425, 1855, 1927, 2194
XceI	RCATGY	4 cut(s)	775, 1375, 1403, 2132
XmiI	GTMKAC	3 cut(s)	742, 789, 2385
XspI	CTAG	3 cut(s)	386, 1199, 1596
Zsp2I	ATGCAT	4 cut(s)	67, 1255, 1687, 1719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.