Rmu_sc0001113.1_g000001

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001113.1
OG0014704
Physical Location & Seq
Reverse (-)
650 .. 6332
5683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001113.1_g000001.1.cds

Sequence Viewer

Length: 3132 bp
atgctagggttaagaaatctcttcctcagggcagtggtttctcaattttctcgtcgttgctcgtcaataacagttatggcagatctcggtctgcttattctcttcctcgtggctctgggtagtgctgagactatgttggtgtcgagggttgggggcgttgttggatccacgtccaagctcgacggtaaagtgcaagatgcgacctcgtttgcagggatggcatctcggtggcgggtgagtggtgatccggtcttggtttggtcaagatcagatcggaaaggtctattccgatcggatccaatggacgatggtgcatctaagaatggcatattgattaaccaaatctggtttggtgttcctattgatagtgataatggatctactcttgatccgtcaatctatggcggaggccaggttactcttgttggaggtggtggtgatctatggtggggttgcgtgcaacctgagtggcgaatctgggcgagctggttatctttggttattgaggagagaaagcatcgggtcttcgatggtagaggcggtaatttgtctcgggttgttgacattaggcttgatctggggggcattggcggtgtttacgacgatggtggaggcgggacagaagctggcatgtatagcggtggcggcttgcttgctttttcatccaagttttgccctaagggttatgattgggctgctagggttttttggccccatacagttcttttgatcacaatgaatagggcaaactggatatgcgagacccagaaaggcataagatcgaaaggtatctttttgggtgataatcctttgaaatacccatctccaatacttcttctgcaacttactatagctgccttggtcaccactctcttccaattcattctcaaccctctcggagaaagtgcttatatctcacaaatgctggcaggtattctattgggaccatcattcttaggaggagaaaatgattttgcagaaatagtttacccagttaaaagtttctacatcagccaaacctttgctttctttggttgcatgctttttctattcctagtgggtgttaaaatggatttgagtatagtcagacggtcgggaaagaaagctgtggtcataggcctctcggcatttttcgtccctttggttctaaatgtaggcttcgctctcgttctgcaacgtacggtcactatggaaccccagttgcacaaatcacttgtagccatagcagtgtttcagtgcctaagtagcttccacgtcatcggttgtctcttgggcgatttgaagctcctaaactcggagcttggtcgtttggctgtgtcttcttcgatgattagcggagtcatctcttggatttgggtcatcctggccttcacagtacggctaagcaccatgaaaaagaccggaaacttgccttttatgggtttaagtttagccgcattgtttgttctaattatattcataatgaggccaataatgctgtggatggttgcccaaacagctaaaggcaaacccattaaagagagctacatagtctccatcttcatcatggtcatgatttgttcccttgtcggtgagattgtgggccagcattttttgcttgggcctatggttttgggcctcgccgtgccagacggcccgcctttggggtcagcattggtggagaaacttgactcgtacgtttccaacattctgttgccgagctactttctgttcactggtgcaagaatcaatttgagtgaaatcagaatgaagactgttgggattgtggaggtgctggctctcagtagcttttgtggtaaggtattagggaccatggtgccttcactctattgcaaaatgcctgctatcgacgcactttcattagggctcatcatgagtgcccaaggcatcaccgaccttcttgtcttgcaacatggaatgctactcttgctcattgacggagaatgctacagcatcatgattatgtcatcggttttatttactggaatcatcacccccatagtgaaatttctgtacaacccctctaagagatacaagtccacaaagcgaagacgaaccattgagtattccttgccaaacatggaactcggacttcttgtctgcatgttccaccaagacagcactccctccatcataaacctaatagaagtcttcaatccaacccccaagagccccatttgcttctgcgttgtccacctcatccgtctctcaggtcgaacagctccagtcctcataacccacagacccgggaaaaacaccgctttacactcaaacaactactccgatcacataatcaatgccttcagattgtaccaagaacacaatgccagcagtgtcataatgaatgcattcaccgcaattgcgccctatgccacaatgcacgacgaggtttgcacacttgcttttgaaaaaaggacttccatgataataatcccatttcacaagtttttcagtgctatccaatgtaccgaagaattagcacatccaatcaggtctgtcaacagaaatattcttcaaaatgctccgtgctccgttgggattcttgtagaccgaggtacgttgaataaaaaaccatccgctttacacacttctatatcaggaagaagtttgtacagtgttgggacgatctttgttgagggacctgatgaccgcgaagcattggcgttagcgacgcggatggctggaaaccccaatgtcagcctaaccgttaccaaatttaccgatcccaagcaaaatagaacccagaaagagatcgattcggaagttataatgaagtataagacagccattgatggcaaaaaacagcacgtctacaaagaggtgcatgttaaagatagcgtcgacatgatcaatgagattagatctatggaggattttttcgacctaattctggtggggagaagacatgatagtgattcgccgttgttcatgggacttaccgaatggaatgagtttccggaactagggttcctcggagacatgttagcatcttttgatgcaaattgcgaggtgtcagtgctggtggtgcagcaacaatcgtttggatgtgctgaaaaagagaagactgatatctttcaatgccatgtgaaggattcgttttctgttgtggatatacctcataatcgacattaa

Protein Analysis

1043

Amino Acids

114.7

Weight (kDa)

8.58

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014704)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39830 FvH4_3g39830
malus_domestica MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica Prupe.6G047700_v2.0.a1
pyrus_communis pycom11g04890
rosa_chinensis RchiOBHm_Chr5g0071771
rosa_laevigata RLG00000036244
rosa_multiflora Rmu_sc0001113.1_g000001
rosa_roxburghii Rroxscaffold_1G00009450
rosa_rugosa Rorug05G0414400 Rorug05G0414500
rosa_samantha Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana Rw5G043720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2768
AasI GACNNNNNNGTC 1 cut(s) 1901
Acc36I ACCTGC 1 cut(s) 920
AccB1I GGYRCC 1 cut(s) 1816
AccI GTMKAC 3 cut(s) 2547, 2811, 2841
AccII CGCG 2 cut(s) 2652, 2674
AccIII TCCGGA 1 cut(s) 2956
AclWI GGATC 8 cut(s) 159, 172, 239, 290, 303, 383, 385, 2717
AcsI RAATTY 2 cut(s) 2006, 2714
AcuI CTGAAG 1 cut(s) 2287
AfaI GTAC 8 cut(s) 1180, 1377, 1676, 2015, 2312, 2467, 2557, 2612
AfiI CCNNNNNNNGG 7 cut(s) 1294, 1418, 1642, 1643, 2890, 2963, 3088
AflIII ACRYGT 1 cut(s) 2979
AgsI TTSAA 7 cut(s) 814, 1282, 2155, 2408, 2516, 2563, 3077
AhdI GACNNNNNGTC 1 cut(s) 2096
AjiI CACGTC 3 cut(s) 171, 1255, 2809
AjnI CCWGG 2 cut(s) 411, 1362
AloI GAACNNNNNNTCC 4 cut(s) 2951, 2952, 2983, 2984
Alw21I GWGCWC 1 cut(s) 2531
Alw26I GTCTC 7 cut(s) 122, 555, 755, 1271, 1537, 2210, 2970
AlwI GGATC 8 cut(s) 159, 172, 239, 290, 303, 383, 385, 2717
AlwNI CAGNNNCTG 1 cut(s) 626
Ama87I CYCGRG 2 cut(s) 552, 2246
Aor13HI TCCGGA 1 cut(s) 2956
AoxI GGCC 9 cut(s) 409, 710, 1117, 1365, 1466, 1582, 1601, 1615, 1633
ApeKI GCWGC 3 cut(s) 695, 854, 3028
ApoI RAATTY 2 cut(s) 2006, 2714
Asp700I GAANNNNTTC 1 cut(s) 2348
AspLEI GCGC 1 cut(s) 2365
AspS9I GGNCC 8 cut(s) 711, 944, 1582, 1601, 1615, 1634, 1809, 2639
AsuC2I CCSGG 2 cut(s) 2247, 2248
AsuHPI GGTGA 9 cut(s) 247, 254, 449, 812, 856, 1583, 1882, 1984, 2344
AvaI CYCGRG 2 cut(s) 552, 2246
AvaII GGWCC 3 cut(s) 944, 1809, 2639
AxyI CCTNAGG 2 cut(s) 26, 678
BaeGI GKGCMC 1 cut(s) 1882
BamHI GGATCC 2 cut(s) 164, 295
BanI GGYRCC 1 cut(s) 1816
BanII GRGCYC 2 cut(s) 1869, 2174
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 7 cut(s) 517, 1311, 1757, 2056, 2143, 2908, 3068
Bbv12I GWGCWC 1 cut(s) 2531
BbvI GCAGC 3 cut(s) 682, 841, 3040
BceAI ACGGC 4 cut(s) 1394, 1607, 1648, 2905
BciT130I CCWGG 2 cut(s) 413, 1364
BclI TGATCA 2 cut(s) 729, 2847
BcnI CCSGG 2 cut(s) 2247, 2248
BcoDI GTCTC 7 cut(s) 122, 555, 755, 1271, 1537, 2210, 2970
BfaI CTAG 4 cut(s) 5, 699, 1055, 2963
BfmI CTRYAG 2 cut(s) 849, 1948
BfuAI ACCTGC 1 cut(s) 920
BglII AGATCT 2 cut(s) 82, 2861
BisI GCNGC 5 cut(s) 646, 696, 855, 1434, 3029
BlpI GCTNAGC 1 cut(s) 1382
BlsI GCNGC 5 cut(s) 647, 697, 856, 1435, 3030
Bme1390I CCNGG 4 cut(s) 413, 1364, 2247, 2248
Bme18I GGWCC 3 cut(s) 944, 1809, 2639
BmeRI GACNNNNNGTC 1 cut(s) 2096
BmeT110I CYCGRG 2 cut(s) 552, 2246
BmgBI CACGTC 3 cut(s) 171, 1255, 2809
BmgT120I GGNCC 8 cut(s) 711, 944, 1582, 1601, 1615, 1634, 1809, 2639
BmiI GGNNCC 9 cut(s) 166, 297, 713, 945, 1194, 1810, 1818, 2640, 2969
BmrFI CCNGG 4 cut(s) 413, 1364, 2247, 2248
BmrI ACTGGG 2 cut(s) 986, 1192
BmsI GCATC 8 cut(s) 187, 230, 323, 526, 1896, 1962, 2986, 2996
BmuI ACTGGG 2 cut(s) 986, 1192
BpiI GAAGAC 7 cut(s) 517, 1311, 1757, 2056, 2143, 2908, 3068
BpmI CTGGAG 1 cut(s) 2208
Bpu1102I GCTNAGC 1 cut(s) 1382
BpuMI CCSGG 2 cut(s) 2247, 2248
Bsa29I ATCGAT 1 cut(s) 2754
BsaBI GATNNNNATC 1 cut(s) 2429
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 6 cut(s) 858, 1812, 1882, 2246, 2551, 2971
BsaWI WCCGGW 3 cut(s) 247, 1400, 2956
BsaXI ACNNNNNCTCC 6 cut(s) 399, 429, 1517, 1547, 2264, 2294
Bsc4I CCNNNNNNNGG 7 cut(s) 1294, 1418, 1642, 1643, 2890, 2963, 3088
Bse1I ACTGG 6 cut(s) 755, 992, 1198, 1720, 1987, 2225
Bse21I CCTNAGG 2 cut(s) 26, 678
Bse8I GATNNNNATC 1 cut(s) 2429
BseAI TCCGGA 1 cut(s) 2956
BseBI CCWGG 2 cut(s) 413, 1364
BseCI ATCGAT 1 cut(s) 2754
BseDI CCNNGG 6 cut(s) 858, 1812, 1882, 2246, 2551, 2971
BseGI GGATG 9 cut(s) 222, 662, 1359, 1488, 2199, 2482, 2573, 2682, 3050
BseJI GATNNNNATC 1 cut(s) 2429
BseLI CCNNNNNNNGG 7 cut(s) 1294, 1418, 1642, 1643, 2890, 2963, 3088
BseMII CTCAG 5 cut(s) 40, 117, 456, 1795, 2223
BseNI ACTGG 6 cut(s) 755, 992, 1198, 1720, 1987, 2225
BseRI GAGGAG 2 cut(s) 521, 975
BseSI GKGCMC 1 cut(s) 1882
BseXI GCAGC 3 cut(s) 682, 841, 3040
BsgI GTGCAG 1 cut(s) 3047
Bsh1236I CGCG 2 cut(s) 2652, 2674
Bsh1285I CGRYCG 2 cut(s) 293, 1094
BshFI GGCC 9 cut(s) 411, 712, 1119, 1367, 1468, 1584, 1603, 1617, 1635
BshNI GGYRCC 1 cut(s) 1816
BshVI ATCGAT 1 cut(s) 2754
BsiEI CGRYCG 2 cut(s) 293, 1094
BsiHKAI GWGCWC 1 cut(s) 2531
BsiHKCI CYCGRG 2 cut(s) 552, 2246
BsiSI CCGG 4 cut(s) 248, 1401, 2247, 2957
BsiWI CGTACG 2 cut(s) 1178, 1674
BslFI GGGAC 7 cut(s) 631, 957, 1121, 1822, 2635, 2652, 2946
BslI CCNNNNNNNGG 7 cut(s) 1294, 1418, 1642, 1643, 2890, 2963, 3088
BsmAI GTCTC 7 cut(s) 122, 555, 755, 1271, 1537, 2210, 2970
BsmBI CGTCTC 1 cut(s) 2210
BsmFI GGGAC 7 cut(s) 631, 957, 1121, 1822, 2635, 2652, 2946
BsmI GAATGC 4 cut(s) 1923, 1949, 2348, 2350
BsnI GGCC 9 cut(s) 411, 712, 1119, 1367, 1468, 1584, 1603, 1617, 1635
Bso31I GGTCTC 1 cut(s) 755
BsoBI CYCGRG 2 cut(s) 552, 2246
Bsp1286I GDGCHC 4 cut(s) 1869, 1882, 2174, 2531
Bsp13I TCCGGA 1 cut(s) 2956
Bsp1407I TGTACA 2 cut(s) 2013, 2610
Bsp1720I GCTNAGC 1 cut(s) 1382
Bsp19I CCATGG 1 cut(s) 1812
BspANI GGCC 9 cut(s) 411, 712, 1119, 1367, 1468, 1584, 1603, 1617, 1635
BspCNI CTCAG 5 cut(s) 39, 118, 457, 1794, 2222
BspDI ATCGAT 1 cut(s) 2754
BspEI TCCGGA 1 cut(s) 2956
BspFNI CGCG 2 cut(s) 2652, 2674
BspHI TCATGA 3 cut(s) 1551, 1872, 1956
BspLI GGNNCC 9 cut(s) 166, 297, 713, 945, 1194, 1810, 1818, 2640, 2969
BspMI ACCTGC 1 cut(s) 920
BspPI GGATC 8 cut(s) 159, 172, 239, 290, 303, 383, 385, 2717
BspT107I GGYRCC 1 cut(s) 1816
BspTNI GGTCTC 1 cut(s) 755
BsrGI TGTACA 2 cut(s) 2013, 2610
BsrI ACTGG 6 cut(s) 755, 992, 1198, 1720, 1987, 2225
BssECI CCNNGG 6 cut(s) 858, 1812, 1882, 2246, 2551, 2971
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 3 cut(s) 858, 1812, 1882
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 413, 1364
Bst4CI ACNGT 9 cut(s) 73, 185, 721, 1092, 1183, 1375, 1756, 2615, 2707
Bst6I CTCTTC 3 cut(s) 26, 107, 878
BstAPI GCANNNNNTGC 1 cut(s) 1594
BstAUI TGTACA 2 cut(s) 2013, 2610
BstDSI CCRYGG 1 cut(s) 1812
BstEII GGTNACC 1 cut(s) 862
BstF5I GGATG 9 cut(s) 222, 662, 1359, 1488, 2199, 2482, 2573, 2682, 3050
BstFNI CGCG 2 cut(s) 2652, 2674
BstHHI GCGC 1 cut(s) 2365
BstMAI GTCTC 7 cut(s) 122, 555, 755, 1271, 1537, 2210, 2970
BstMCI CGRYCG 2 cut(s) 293, 1094
BstNI CCWGG 2 cut(s) 413, 1364
BstNSI RCATGY 5 cut(s) 634, 1042, 2107, 2828, 2983
BstPI GGTNACC 1 cut(s) 862
BstSCI CCNGG 4 cut(s) 411, 1362, 2245, 2246
BstSFI CTRYAG 2 cut(s) 849, 1948
BstSLI GKGCMC 1 cut(s) 1882
BstUI CGCG 2 cut(s) 2652, 2674
BstV1I GCAGC 3 cut(s) 682, 841, 3040
BstV2I GAAGAC 7 cut(s) 517, 1311, 1757, 2056, 2143, 2908, 3068
BstX2I RGATCY 5 cut(s) 82, 164, 295, 377, 2861
BstYI RGATCY 5 cut(s) 82, 164, 295, 377, 2861
Bsu15I ATCGAT 1 cut(s) 2754
Bsu36I CCTNAGG 2 cut(s) 26, 678
BsuRI GGCC 9 cut(s) 411, 712, 1119, 1367, 1468, 1584, 1603, 1617, 1635
BsuTUI ATCGAT 1 cut(s) 2754
BtgI CCRYGG 1 cut(s) 1812
BtrI CACGTC 3 cut(s) 171, 1255, 2809
BtsCI GGATG 9 cut(s) 222, 662, 1359, 1488, 2199, 2482, 2573, 2682, 3050
BtsI GCAGTG 3 cut(s) 39, 1233, 2338
BtsIMutI CAGTG 8 cut(s) 39, 1233, 1241, 1713, 2338, 2458, 2620, 3021
BveI ACCTGC 1 cut(s) 920
CaiI CAGNNNCTG 1 cut(s) 626
CciI TCATGA 3 cut(s) 1551, 1872, 1956
CfoI GCGC 1 cut(s) 2365
Cfr13I GGNCC 8 cut(s) 711, 944, 1582, 1601, 1615, 1634, 1809, 2639
Cfr9I CCCGGG 1 cut(s) 2246
ClaI ATCGAT 1 cut(s) 2754
CseI GACGC 3 cut(s) 1859, 2680, 2827
Csp6I GTAC 8 cut(s) 1179, 1376, 1675, 2014, 2311, 2466, 2556, 2611
CspCI CAANNNNNGTGG 2 cut(s) 449, 484
CviQI GTAC 8 cut(s) 1179, 1376, 1675, 2014, 2311, 2466, 2556, 2611
DrdI GACNNNNNNGTC 1 cut(s) 1901
DriI GACNNNNNGTC 1 cut(s) 2096
DseDI GACNNNNNNGTC 1 cut(s) 1901
Eam1104I CTCTTC 3 cut(s) 26, 107, 878
Eam1105I GACNNNNNGTC 1 cut(s) 2096
EarI CTCTTC 3 cut(s) 26, 107, 878
EciI GGCGGA 1 cut(s) 420
Eco130I CCWWGG 3 cut(s) 858, 1812, 1882
Eco147I AGGCCT 1 cut(s) 1119
Eco24I GRGCYC 2 cut(s) 1869, 2174
Eco31I GGTCTC 1 cut(s) 755
Eco32I GATATC 1 cut(s) 3070
Eco47I GGWCC 3 cut(s) 944, 1809, 2639
Eco57I CTGAAG 1 cut(s) 2287
Eco81I CCTNAGG 2 cut(s) 26, 678
Eco88I CYCGRG 2 cut(s) 552, 2246
Eco91I GGTNACC 1 cut(s) 862
EcoO109I RGGNCCY 1 cut(s) 2639
EcoO65I GGTNACC 1 cut(s) 862
EcoRII CCWGG 2 cut(s) 411, 1362
EcoRV GATATC 1 cut(s) 3070
EcoT14I CCWWGG 3 cut(s) 858, 1812, 1882
EcoT22I ATGCAT 1 cut(s) 2350
EcoT38I GRGCYC 2 cut(s) 1869, 2174
ErhI CCWWGG 3 cut(s) 858, 1812, 1882
Esp3I CGTCTC 1 cut(s) 2210
FaqI GGGAC 7 cut(s) 631, 957, 1121, 1822, 2635, 2652, 2946
FauI CCCGC 3 cut(s) 225, 608, 1644
FbaI TGATCA 2 cut(s) 729, 2847
FblI GTMKAC 3 cut(s) 2547, 2811, 2841
Fnu4HI GCNGC 5 cut(s) 646, 696, 855, 1434, 3029
FokI GGATG 9 cut(s) 229, 649, 1346, 1495, 2186, 2469, 2560, 2689, 3057
FriOI GRGCYC 2 cut(s) 1869, 2174
Fsp4HI GCNGC 5 cut(s) 646, 696, 855, 1434, 3029
FspBI CTAG 4 cut(s) 5, 699, 1055, 2963
GlaI GCGC 1 cut(s) 2364
GluI GCNGC 5 cut(s) 646, 696, 855, 1434, 3029
GsuI CTGGAG 1 cut(s) 2208
HaeIII GGCC 9 cut(s) 411, 712, 1119, 1367, 1468, 1584, 1603, 1617, 1635
HapII CCGG 4 cut(s) 248, 1401, 2247, 2957
HgaI GACGC 3 cut(s) 1859, 2680, 2827
HhaI GCGC 1 cut(s) 2365
Hin6I GCGC 1 cut(s) 2363
HinP1I GCGC 1 cut(s) 2363
HincII GTYRAC 3 cut(s) 562, 2500, 2842
HindII GTYRAC 3 cut(s) 562, 2500, 2842
HinfI GANTC 9 cut(s) 474, 1338, 1670, 1725, 1986, 2539, 2756, 2915, 3092
HpaII CCGG 4 cut(s) 248, 1401, 2247, 2957
HphI GGTGA 9 cut(s) 247, 254, 449, 812, 856, 1583, 1882, 1984, 2344
Hpy188III TCNNGA 8 cut(s) 264, 386, 1095, 1552, 1873, 1957, 2598, 2957
Hpy99I CGWCG 7 cut(s) 57, 185, 605, 1853, 2387, 2674, 2843
HpyAV CCTTC 5 cut(s) 1378, 1830, 1907, 2311, 3082
HpyCH4III ACNGT 9 cut(s) 73, 185, 721, 1092, 1183, 1375, 1756, 2615, 2707
HpyCH4IV ACGT 6 cut(s) 170, 1177, 1254, 1677, 2558, 2808
HpySE526I ACGT 6 cut(s) 170, 1177, 1254, 1677, 2558, 2808
HspAI GCGC 1 cut(s) 2363
Kpn2I TCCGGA 1 cut(s) 2956
Ksp22I TGATCA 2 cut(s) 729, 2847
LmnI GCTCC 5 cut(s) 1290, 1297, 2227, 2527, 2534
Lsp1109I GCAGC 3 cut(s) 682, 841, 3040
LweI GCATC 8 cut(s) 187, 230, 323, 526, 1896, 1962, 2986, 2996
MaeI CTAG 4 cut(s) 5, 699, 1055, 2963
MaeII ACGT 6 cut(s) 170, 1177, 1254, 1677, 2558, 2808
MaeIII GTNAC 4 cut(s) 415, 862, 1183, 2707
MfeI CAATTG 1 cut(s) 2358
MflI RGATCY 5 cut(s) 82, 164, 295, 377, 2861
MhlI GDGCHC 4 cut(s) 1869, 1882, 2174, 2531
MlyI GAGTC 2 cut(s) 1347, 1664
MmeI TCCRAC 4 cut(s) 142, 406, 1707, 2183
Mph1103I ATGCAT 1 cut(s) 2350
MroI TCCGGA 1 cut(s) 2956
MroXI GAANNNNTTC 1 cut(s) 2348
MseI TTAA 8 cut(s) 11, 336, 996, 1065, 1424, 1515, 2829, 3130
MslI CAYNNNNRTG 5 cut(s) 226, 1226, 1550, 1961, 2910
MspI CCGG 4 cut(s) 248, 1401, 2247, 2957
MspR9I CCNGG 4 cut(s) 413, 1364, 2247, 2248
MunI CAATTG 1 cut(s) 2358
Mva1269I GAATGC 4 cut(s) 1923, 1949, 2348, 2350
MvaI CCWGG 2 cut(s) 413, 1364
MvnI CGCG 2 cut(s) 2652, 2674
NciI CCSGG 2 cut(s) 2247, 2248
NcoI CCATGG 1 cut(s) 1812
NlaIV GGNNCC 9 cut(s) 166, 297, 713, 945, 1194, 1810, 1818, 2640, 2969
NmeAIII GCCGAG 2 cut(s) 1103, 1722
NmuCI GTSAC 2 cut(s) 862, 1183
NsiI ATGCAT 1 cut(s) 2350
NspI RCATGY 5 cut(s) 634, 1042, 2107, 2828, 2983
PaeI GCATGC 1 cut(s) 1042
PagI TCATGA 3 cut(s) 1551, 1872, 1956
PceI AGGCCT 1 cut(s) 1119
PciI ACATGT 1 cut(s) 2979
PctI GAATGC 4 cut(s) 1923, 1949, 2348, 2350
PdmI GAANNNNTTC 1 cut(s) 2348
PfeI GAWTC 7 cut(s) 474, 1725, 1986, 2539, 2756, 2915, 3092
Pfl23II CGTACG 2 cut(s) 1178, 1674
PkrI GCNGC 5 cut(s) 647, 697, 856, 1435, 3030
Ple19I CGATCG 1 cut(s) 293
PleI GAGTC 2 cut(s) 1346, 1664
PpsI GAGTC 2 cut(s) 1346, 1664
PpuMI RGGWCCY 1 cut(s) 2639
PscI ACATGT 1 cut(s) 2979
PsiI TTATAA 1 cut(s) 2768
Psp5II RGGWCCY 1 cut(s) 2639
Psp6I CCWGG 2 cut(s) 411, 1362
PspEI GGTNACC 1 cut(s) 862
PspGI CCWGG 2 cut(s) 411, 1362
PspLI CGTACG 2 cut(s) 1178, 1674
PspN4I GGNNCC 9 cut(s) 166, 297, 713, 945, 1194, 1810, 1818, 2640, 2969
PspPI GGNCC 8 cut(s) 711, 944, 1582, 1601, 1615, 1634, 1809, 2639
PspPPI RGGWCCY 1 cut(s) 2639
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 5 cut(s) 82, 164, 295, 377, 2861
PvuI CGATCG 1 cut(s) 293
RsaI GTAC 8 cut(s) 1180, 1377, 1676, 2015, 2312, 2467, 2557, 2612
RsaNI GTAC 8 cut(s) 1179, 1376, 1675, 2014, 2311, 2466, 2556, 2611
RseI CAYNNNNRTG 5 cut(s) 226, 1226, 1550, 1961, 2910
SalI GTCGAC 1 cut(s) 2840
SaqAI TTAA 8 cut(s) 11, 336, 996, 1065, 1424, 1515, 2829, 3130
SatI GCNGC 5 cut(s) 646, 696, 855, 1434, 3029
Sau96I GGNCC 8 cut(s) 711, 944, 1582, 1601, 1615, 1634, 1809, 2639
SchI GAGTC 2 cut(s) 1347, 1664
ScrFI CCNGG 4 cut(s) 413, 1364, 2247, 2248
SduI GDGCHC 4 cut(s) 1869, 1882, 2174, 2531
SfaNI GCATC 8 cut(s) 187, 230, 323, 526, 1896, 1962, 2986, 2996
SfcI CTRYAG 2 cut(s) 849, 1948
SinI GGWCC 3 cut(s) 944, 1809, 2639
SmaI CCCGGG 1 cut(s) 2248
SmiMI CAYNNNNRTG 5 cut(s) 226, 1226, 1550, 1961, 2910
SphI GCATGC 1 cut(s) 1042
SseBI AGGCCT 1 cut(s) 1119
SspI AATATT 1 cut(s) 2509
SspMI CTAG 4 cut(s) 5, 699, 1055, 2963
StuI AGGCCT 1 cut(s) 1119
StyD4I CCNGG 4 cut(s) 411, 1362, 2245, 2246
StyI CCWWGG 3 cut(s) 858, 1812, 1882
TaaI ACNGT 9 cut(s) 73, 185, 721, 1092, 1183, 1375, 1756, 2615, 2707
TaiI ACGT 6 cut(s) 173, 1180, 1257, 1680, 2561, 2811
TaqII GACCGA 2 cut(s) 77, 2565
TatI WGTACW 2 cut(s) 2013, 2610
TauI GCSGC 2 cut(s) 648, 1436
TfiI GAWTC 7 cut(s) 474, 1725, 1986, 2539, 2756, 2915, 3092
Tru1I TTAA 8 cut(s) 11, 336, 996, 1065, 1424, 1515, 2829, 3130
Tru9I TTAA 8 cut(s) 11, 336, 996, 1065, 1424, 1515, 2829, 3130
TscAI CASTG 8 cut(s) 39, 1233, 1241, 1720, 2338, 2458, 2620, 3021
TseFI GTSAC 2 cut(s) 862, 1183
TseI GCWGC 3 cut(s) 695, 854, 3028
Tsp45I GTSAC 2 cut(s) 862, 1183
TspGWI ACGGA 5 cut(s) 381, 1953, 2192, 2514, 2521
TspMI CCCGGG 1 cut(s) 2246
TspRI CASTG 8 cut(s) 39, 1233, 1241, 1720, 2338, 2458, 2620, 3021
VpaK11BI GGWCC 3 cut(s) 944, 1809, 2639
XapI RAATTY 2 cut(s) 2006, 2714
XceI RCATGY 5 cut(s) 634, 1042, 2107, 2828, 2983
XcmI CCANNNNNNNNNTGG 3 cut(s) 347, 1476, 1543
XmaI CCCGGG 1 cut(s) 2246
XmiI GTMKAC 3 cut(s) 2547, 2811, 2841
XmnI GAANNNNTTC 1 cut(s) 2348
XspI CTAG 4 cut(s) 5, 699, 1055, 2963
Zsp2I ATGCAT 1 cut(s) 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.