Rorug05G0414400

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014704
Physical Location & Seq
Reverse (-)
57273816 .. 57274457
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0414400.1

Sequence Viewer

Length: 642 bp
ATGGCTACTGAAAATTGGCAAGTGGTGGGTGACATCACCTGCAACCCAAAATACCTAGGTCTTGTTCAACCTGAAAAGGAGCTGCCTAACACCAAATTCTTGATGGATTTCAATGTCAAGATGAGGCGCGCGCAGTCTACTGTTTTGGAGGATGATGAAGAAATTGTAGAAGAGAAGAATCTGCGCTCAGTCGAACTTGAGTACAATCCACATAGCATGTGGGTTGCCATAAATAAAGAGCTTCAAGACCTGCAAGTTCCAATCCAAGTTCGTGCAGCCATTGAAGATCCAATACTCAAGGGAACCAGAGTCGCACGAGCCGATAGCTCTTATGACGCTCGCAAGTATCTGCATATGGAGGTGGCTATCGATTTTCGTGTTGGCGTTTTTGATGGTATTGGTGAATATGAGCCTACGTTTGTGCCGGCAAGTGAAGCGTCCATTAAGAAACTGAAGAAGACAAGAGCCGAAGTGTCGTCGACAATGTGCACGGTTTGTATGGAGGAGATGATGGTTGGTTCGGAAGCGACTCGTATGCCTTGTTCCCATCTTTACCATGAGAGTTGCATTGTAGAGTGGCTGCAGAAAAGTGGGGTTTGCCCATCCTGTAGGTTCCTCATGCCCACTGATGATGATGAATGA

Protein Analysis

213

Amino Acids

24.29

Weight (kDa)

4.88

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 162 - 204 6.2e-14 Ring finger domain
zf-C3HC4_2 PF13923 162 - 203 3.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 163 - 190 1e-08 RING-like zinc finger
zf-C3HC4 PF00097 163 - 203 3.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 163 - 204 9.2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014704)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39830 FvH4_3g39830
malus_domestica MD03G1057100.v1.1 MD11G1059100.v1.1
prunus_persica Prupe.6G047700_v2.0.a1
pyrus_communis pycom11g04890
rosa_chinensis RchiOBHm_Chr5g0071771
rosa_laevigata RLG00000036244
rosa_multiflora Rmu_sc0001113.1_g000001
rosa_roxburghii Rroxscaffold_1G00009450
rosa_rugosa Rorug05G0414400 Rorug05G0414500
rosa_samantha Rh5AG470500 Rh5BG490200 Rh5CG514200 Rh5DG500900
rosa_wichuraiana Rw5G043720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 2 cut(s) 47, 258
AccI GTMKAC 2 cut(s) 137, 479
AccII CGCG 2 cut(s) 129, 131
AclWI GGATC 1 cut(s) 281
AcsI RAATTY 1 cut(s) 95
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 1 cut(s) 203
AgsI TTSAA 4 cut(s) 68, 112, 245, 284
AluBI AGCT 3 cut(s) 82, 241, 327
AluI AGCT 3 cut(s) 82, 241, 327
Alw21I GWGCWC 1 cut(s) 491
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 1 cut(s) 281
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 3 cut(s) 82, 275, 580
ApoI RAATTY 1 cut(s) 95
AspA2I CCTAGG 1 cut(s) 55
AspLEI GCGC 4 cut(s) 129, 131, 133, 186
AsuHPI GGTGA 3 cut(s) 28, 41, 413
AvrII CCTAGG 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 491
BarI GAAGNNNNNNTAC 2 cut(s) 276, 308
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 3 cut(s) 69, 287, 567
BccI CCATC 5 cut(s) 97, 386, 505, 555, 610
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 2 cut(s) 581, 607
BfuAI ACCTGC 2 cut(s) 47, 258
BisI GCNGC 3 cut(s) 83, 276, 581
BlnI CCTAGG 1 cut(s) 55
BlsI GCNGC 3 cut(s) 84, 277, 582
BmiI GGNNCC 2 cut(s) 304, 614
BpiI GAAGAC 1 cut(s) 464
BpuEI CTTGAG 2 cut(s) 218, 281
Bsa29I ATCGAT 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 424
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 2 cut(s) 157, 602
BseMII CTCAG 1 cut(s) 201
BsePI GCGCGC 2 cut(s) 127, 129
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 3 cut(s) 69, 287, 567
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 2 cut(s) 129, 131
BshVI ATCGAT 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 1 cut(s) 286
BspCNI CTCAG 1 cut(s) 200
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 2 cut(s) 129, 131
BspLI GGNNCC 2 cut(s) 304, 614
BspMAI CTGCAG 1 cut(s) 585
BspMI ACCTGC 2 cut(s) 47, 258
BspPI GGATC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 55
BssHII GCGCGC 2 cut(s) 127, 129
BssMI GATC 1 cut(s) 286
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 55
Bst2BI CACGAG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 142, 493
Bst6I CTCTTC 1 cut(s) 165
BstC8I GCNNGC 4 cut(s) 129, 131, 340, 426
BstDEI CTNAG 1 cut(s) 187
BstF5I GGATG 2 cut(s) 157, 602
BstFNI CGCG 2 cut(s) 129, 131
BstHHI GCGC 4 cut(s) 129, 131, 133, 186
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 220
BstSFI CTRYAG 2 cut(s) 581, 607
BstSLI GKGCMC 1 cut(s) 491
BstUI CGCG 2 cut(s) 129, 131
BstV1I GCAGC 3 cut(s) 69, 287, 567
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 369
BsuTUI ATCGAT 1 cut(s) 369
BtsCI GGATG 2 cut(s) 157, 602
BtsIMutI CAGTG 1 cut(s) 624
BveI ACCTGC 2 cut(s) 47, 258
Cac8I GCNNGC 4 cut(s) 129, 131, 340, 426
CfoI GCGC 4 cut(s) 129, 131, 133, 186
Cfr10I RCCGGY 1 cut(s) 424
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 2 cut(s) 344, 426
Csp6I GTAC 1 cut(s) 202
CviAII CATG 3 cut(s) 217, 557, 619
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 187
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 473
EcoT14I CCWWGG 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 3 cut(s) 220, 560, 622
FatI CATG 3 cut(s) 216, 556, 618
FauNDI CATATG 1 cut(s) 354
FblI GTMKAC 2 cut(s) 137, 479
Fnu4HI GCNGC 3 cut(s) 83, 276, 581
FokI GGATG 2 cut(s) 164, 589
Fsp4HI GCNGC 3 cut(s) 83, 276, 581
FspBI CTAG 1 cut(s) 56
GlaI GCGC 4 cut(s) 128, 130, 132, 185
GluI GCNGC 3 cut(s) 83, 276, 581
HapII CCGG 1 cut(s) 425
HgaI GACGC 2 cut(s) 344, 426
HhaI GCGC 4 cut(s) 129, 131, 133, 186
Hin1II CATG 3 cut(s) 220, 560, 622
Hin6I GCGC 4 cut(s) 127, 129, 131, 184
HinP1I GCGC 4 cut(s) 127, 129, 131, 184
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 3 cut(s) 178, 309, 529
HpaII CCGG 1 cut(s) 425
HphI GGTGA 3 cut(s) 28, 41, 413
Hpy166II GTNNAC 3 cut(s) 138, 480, 489
Hpy188I TCNGA 1 cut(s) 523
Hpy188III TCNNGA 3 cut(s) 100, 118, 245
Hpy8I GTNNAC 3 cut(s) 138, 480, 489
Hpy99I CGWCG 1 cut(s) 481
HpyCH4III ACNGT 2 cut(s) 142, 493
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 7 cut(s) 42, 253, 275, 352, 489, 567, 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 1 cut(s) 187
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 3 cut(s) 220, 560, 622
HspAI GCGC 4 cut(s) 127, 129, 131, 184
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 6 cut(s) 52, 84, 263, 319, 438, 619
Lsp1109I GCAGC 3 cut(s) 69, 287, 567
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 1 cut(s) 29
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 6 cut(s) 170, 182, 187, 296, 466, 469
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 3 cut(s) 13, 95, 162
MlyI GAGTC 2 cut(s) 318, 523
MnlI CCTC 5 cut(s) 117, 142, 352, 496, 626
MroNI GCCGGC 1 cut(s) 424
MseI TTAA 1 cut(s) 444
MspI CCGG 1 cut(s) 425
MvnI CGCG 2 cut(s) 129, 131
MwoI GCNNNNNNNGC 1 cut(s) 434
NaeI GCCGGC 1 cut(s) 426
NdeI CATATG 1 cut(s) 354
NdeII GATC 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 3 cut(s) 220, 560, 622
NlaIV GGNNCC 2 cut(s) 304, 614
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 47
PauI GCGCGC 2 cut(s) 127, 129
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 3 cut(s) 84, 277, 582
PleI GAGTC 2 cut(s) 317, 523
PpsI GAGTC 2 cut(s) 317, 523
PspN4I GGNNCC 2 cut(s) 304, 614
PsrI GAACNNNNNNTAC 2 cut(s) 526, 558
PstI CTGCAG 1 cut(s) 585
PsuI RGATCY 1 cut(s) 286
PteI GCGCGC 2 cut(s) 127, 129
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 478
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 3 cut(s) 83, 276, 581
Sau3AI GATC 1 cut(s) 286
SchI GAGTC 2 cut(s) 318, 523
SduI GDGCHC 1 cut(s) 491
SfcI CTRYAG 2 cut(s) 581, 607
SmlI CTYRAG 2 cut(s) 197, 296
SmoI CTYRAG 2 cut(s) 197, 296
Sse9I AATT 3 cut(s) 13, 95, 162
SspMI CTAG 1 cut(s) 56
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 142, 493
TaiI ACGT 1 cut(s) 419
TaqI TCGA 3 cut(s) 192, 369, 479
TasI AATT 3 cut(s) 13, 95, 162
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 1 cut(s) 631
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 3 cut(s) 82, 275, 580
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 171
TspRI CASTG 1 cut(s) 631
VneI GTGCAC 1 cut(s) 487
XapI RAATTY 1 cut(s) 95
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 2 cut(s) 100, 216
XmaJI CCTAGG 1 cut(s) 55
XmiI GTMKAC 2 cut(s) 137, 479
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.