FvH4_3g42970

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008402
Physical Location & Seq
Forward (+)
35907826 .. 35912427
4602 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42970.t1

Sequence Viewer

Length: 2136 bp
ATGAACACTCACCGCCCGGTGAGGGTACTCTCACGTGCCTTGTCACGTGCCCTATCCCACTCCTCCTCCGCCGCCGCGGAACCCTCCTTCCCTTTGAAAAACGTGACCAAATCAAACTTCGAGCAGGTCCTCGCCGAGCTCCGCCGCCATGTCGCCGCCGCCGACTTCGTGGCCGTCGACCTCGAGATGAGCGGGGTCACCAGCGCGCCGTGGAGGGACTCGTTAGAGTTCGACCGCCCTGACGTCAGCTACCTGAAGGTGAAGGACTCGGCGGAGAAGTTCGCCGTGCTCCAGTTCGGCGTCTGCCCCTTCCGCTGGGACTCGTCCAAGAAATCCTTCATTGCTCATCCGCACAATTTCTATATCTTCCCACGTCAAGAGCTTCCTGTCGAAAGCCCCACTTATGAGTTTCTTTGCCAGACTACGTCAATTGACTTCTTAGCCAAATACCAGTTCGATTTCAATGTGTGCATACATGAAGGAATATCTTATTTATCCAGAGGGCAGGAAAGTAAAGCACTGAGGCGTTTACGTTTGGCATATGAGGATGAATCTAACTTGAAAGAAGTAAGGGATGCCCCTTTGGTTAAGATGGCTGACACTCTCTTCACTGAACGGATGAAGAATACATTTACTGAGTGGCGTGATGGGTTGTTGAGAAATAGAAATGGAGGGTCAAAATTTCAGGGAATCTCAAATGACCGCCAACAACATTTAGAAACGATTTTCTTCAAGATGTGTCCAGCTCTTAGTCTAAATGGATTTACATCTCATCAGCTTAGGTTGATTCAACTGGTCATCAGAAGACATTTCCAAGATCTTTCTTATGTTCAGGTAAATGGGGAAAATTCCTGTCAACAGCATCTAGTGGTGCATACAGATTCTGAGGATGATAGAGAGTCACTTATGAAAGAGGTGAAGGATGAGCATCTTAGACAAGCAGAATCCAAGGTTCAAGCTGCCGTGGGGTTTCGTCATGTTATTGACCTTCTTTCCTCAGAAAAAAAGCTGATTGTTGATATTGCACATATATACAGCAAATTTGTAGGTCCTCTTCCTTCAACTGCTGAAGAATTCGTCTCTTCTGTTAACGAGTGGTTTCCACACATCATTGACACCAAATTACTCTTGAATGCTAATACTGTGCTTCAACAACGGATGAAGAAATCCCAAACATCTCTCTCGTCAGCATTTGCATCATTATGTCCACAAATGTCTCTGGGGGCGAAAAGCACTGATCTGGCATTCCAATTGTGTGTCAAAGTTGAGGTTCAGGTTGATGATTTGAGGTCCTCCAACTGGAATTCTGGAGCAAAACATGAAGCTGGATATGATGCTTTTATGACAGGGTGCGTCTTTGCTCAGGCATGTGGACATCTAGGTATTGATTTTGAAGCCTGTTTGCCCTCTGAAAAGTTGGCCCACAATGAAAAGCTCAAACAGCACATCAACCTTCTATTCCTTAGTTGGAATAAAGGAGATGTTATTGATTTAACTACGGGAAATAAGAATGCGATGTCTGTGGGATCTAAGAAAAAACAGCGTTACTCGGAGATTTCGTTTGAGAAAATTGCTTTGATATGGGGATTTTCATCCAAGGTCAAGGCACAAGAGATAAGAGAATGCATTTCCAAAGTTTTTGGCCCGTCGTCGGTTGTATCTATCTATCACTTGGATGAATCTGCAGTGTTCGTTCAGTTTAAGAAGCAGAAATTGGTCTCTGATTTTCTCAGTTTGAAGGACACATTAGAGAGAAATAATGGTCCTGTCGCTGTTTTGCATCCTTTTGCAGGACTTCTGGAAGGTGGAAATACCCGAGCTGCTGATTATGAAACTTACAAAGAAATTTGTAGTTCACCCAACGCAAAAGTCTTGTTTGCAGATCAGGCTGAGGCATTTGGTATCCAATGGAAAACCAAATTGGTTGAAGCTAAGGCATCATTGGATAGCCTAGAATGTGAGAGTTCAGGTGTACAAAACAATGCTTTAGATCCTGCTTCAAAGGTCGACGAGAAATCCGAAAGAAAAGACATGGACAATGTGCAAAGTGATTCATCCAGTGCACATGTCTCGCACTTTAAGATTATAGATTCTTTGATTGCTTCTAGAGCTGACAAGGTCAGAACTAATATGTGA

Protein Analysis

712

Amino Acids

79.98

Weight (kDa)

6.92

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 35 - 453 3.6e-95 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 115
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 2 cut(s) 177, 2007
AccII CGCG 2 cut(s) 77, 206
AclWI GGATC 2 cut(s) 1534, 1985
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 6 cut(s) 680, 847, 1040, 1073, 1303, 1845
AcuI CTGAAG 2 cut(s) 275, 1089
AcvI CACGTG 2 cut(s) 35, 47
AcyI GRCGYC 2 cut(s) 243, 300
AfaI GTAC 2 cut(s) 27, 1974
AfiI CCNNNNNNNGG 5 cut(s) 22, 315, 1299, 1651, 1790
AflIII ACRYGT 1 cut(s) 2065
AjiI CACGTC 1 cut(s) 374
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
Alw21I GWGCWC 3 cut(s) 141, 291, 2065
Alw26I GTCTC 4 cut(s) 1084, 1221, 1723, 2074
Alw44I GTGCAC 1 cut(s) 2061
AlwI GGATC 2 cut(s) 1534, 1985
AlwNI CAGNNNCTG 1 cut(s) 884
Ama87I CYCGRG 2 cut(s) 182, 1815
AoxI GGCC 3 cut(s) 171, 1419, 1642
ApaLI GTGCAC 1 cut(s) 2061
ApeKI GCWGC 2 cut(s) 959, 1820
ApoI RAATTY 6 cut(s) 680, 847, 1040, 1073, 1303, 1845
Asp700I GAANNNNTTC 1 cut(s) 335
AspLEI GCGC 2 cut(s) 206, 208
AspS9I GGNCC 6 cut(s) 127, 1049, 1290, 1420, 1643, 1763
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 5 cut(s) 31, 190, 271, 928, 1848
AvaI CYCGRG 2 cut(s) 182, 1815
AvaII GGWCC 4 cut(s) 127, 1049, 1290, 1763
BaeGI GKGCMC 2 cut(s) 52, 2065
BanII GRGCYC 1 cut(s) 141
BarI GAAGNNNNNNTAC 2 cut(s) 1038, 1070
BbrPI CACGTG 2 cut(s) 35, 47
BbsI GAAGAC 1 cut(s) 811
Bbv12I GWGCWC 3 cut(s) 141, 291, 2065
BbvCI CCTCAGC 1 cut(s) 1890
BbvI GCAGC 2 cut(s) 946, 1807
BccI CCATC 2 cut(s) 586, 641
BceAI ACGGC 4 cut(s) 158, 193, 269, 947
BcgI CGANNNNNNTGC 2 cut(s) 2052, 2086
BciVI GTATCC 1 cut(s) 1913
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 4 cut(s) 1084, 1221, 1723, 2074
BfaI CTAG 4 cut(s) 866, 1379, 1952, 2106
BfmI CTRYAG 1 cut(s) 1683
BfuAI ACCTGC 1 cut(s) 115
BfuI GTATCC 1 cut(s) 1913
BglII AGATCT 1 cut(s) 817
BisI GCNGC 7 cut(s) 72, 75, 145, 156, 159, 960, 1821
BlsI GCNGC 7 cut(s) 73, 76, 146, 157, 160, 961, 1822
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 4 cut(s) 127, 1049, 1290, 1763
BmeT110I CYCGRG 2 cut(s) 182, 1815
BmgBI CACGTC 1 cut(s) 374
BmgT120I GGNCC 6 cut(s) 127, 1049, 1290, 1420, 1643, 1763
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 7 cut(s) 565, 871, 937, 1205, 1324, 1789, 1946
BpiI GAAGAC 1 cut(s) 811
BpmI CTGGAG 2 cut(s) 275, 1329
Bpu10I CCTNAGC 4 cut(s) 779, 1362, 1890, 1932
BpuMI CCSGG 1 cut(s) 17
BsaAI YACGTR 2 cut(s) 35, 47
BsaBI GATNNNNATC 3 cut(s) 766, 927, 1591
BsaHI GRCGYC 2 cut(s) 243, 300
BsaI GGTCTC 1 cut(s) 1723
BsaJI CCNNGG 5 cut(s) 75, 209, 948, 963, 1596
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 1544, 1574
Bsc4I CCNNNNNNNGG 5 cut(s) 22, 315, 1299, 1651, 1790
Bse1I ACTGG 5 cut(s) 292, 451, 798, 1304, 2058
Bse3DI GCAATG 1 cut(s) 339
Bse8I GATNNNNATC 3 cut(s) 766, 927, 1591
BseDI CCNNGG 5 cut(s) 75, 209, 948, 963, 1596
BseJI GATNNNNATC 3 cut(s) 766, 927, 1591
BseLI CCNNNNNNNGG 5 cut(s) 22, 315, 1299, 1651, 1790
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 7 cut(s) 512, 627, 876, 1011, 1376, 1744, 1881
BseNI ACTGG 5 cut(s) 292, 451, 798, 1304, 2058
BsePI GCGCGC 1 cut(s) 204
BseRI GAGGAG 2 cut(s) 52, 55
BseSI GKGCMC 2 cut(s) 52, 2065
BseXI GCAGC 2 cut(s) 946, 1807
BseYI CCCAGC 1 cut(s) 315
Bsh1236I CGCG 2 cut(s) 77, 206
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 3 cut(s) 173, 1421, 1644
BsiEI CGRYCG 1 cut(s) 235
BsiHKAI GWGCWC 3 cut(s) 141, 291, 2065
BsiHKCI CYCGRG 2 cut(s) 182, 1815
BsiSI CCGG 1 cut(s) 17
BslFI GGGAC 2 cut(s) 230, 332
BslI CCNNNNNNNGG 5 cut(s) 22, 315, 1299, 1651, 1790
BsmAI GTCTC 4 cut(s) 1084, 1221, 1723, 2074
BsmBI CGTCTC 1 cut(s) 1084
BsmFI GGGAC 2 cut(s) 230, 332
BsmI GAATGC 4 cut(s) 1138, 1244, 1516, 1628
BsnI GGCC 3 cut(s) 173, 1421, 1644
Bso31I GGTCTC 1 cut(s) 1723
BsoBI CYCGRG 2 cut(s) 182, 1815
Bsp1286I GDGCHC 4 cut(s) 52, 141, 291, 2065
Bsp1407I TGTACA 1 cut(s) 1972
Bsp143I GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
BspANI GGCC 3 cut(s) 173, 1421, 1644
BspCNI CTCAG 7 cut(s) 513, 628, 877, 1010, 1375, 1743, 1882
BspFNI CGCG 2 cut(s) 77, 206
BspLI GGNNCC 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 1687
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 2 cut(s) 1534, 1985
BspTNI GGTCTC 1 cut(s) 1723
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 339
BsrGI TGTACA 1 cut(s) 1972
BsrI ACTGG 5 cut(s) 292, 451, 798, 1304, 2058
BssECI CCNNGG 5 cut(s) 75, 209, 948, 963, 1596
BssHII GCGCGC 1 cut(s) 204
BssMI GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
BssNI GRCGYC 2 cut(s) 243, 300
BssT1I CCWWGG 2 cut(s) 948, 1596
Bst4CI ACNGT 1 cut(s) 1144
Bst6I CTCTTC 3 cut(s) 611, 1059, 1087
BstACI GRCGYC 2 cut(s) 243, 300
BstAUI TGTACA 1 cut(s) 1972
BstBAI YACGTR 2 cut(s) 35, 47
BstC8I GCNNGC 1 cut(s) 206
BstDSI CCRYGG 3 cut(s) 75, 209, 963
BstEII GGTNACC 1 cut(s) 196
BstENI CCTNNNNNAGG 1 cut(s) 1788
BstFNI CGCG 2 cut(s) 77, 206
BstHHI GCGC 2 cut(s) 206, 208
BstKTI GATC 5 cut(s) 820, 1240, 1529, 1885, 1993
BstMAI GTCTC 4 cut(s) 1084, 1221, 1723, 2074
BstMBI GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 4 cut(s) 312, 1441, 1886, 2108
BstNSI RCATGY 2 cut(s) 1371, 2069
BstPI GGTNACC 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 1683
BstSLI GKGCMC 2 cut(s) 52, 2065
BstUI CGCG 2 cut(s) 77, 206
BstV1I GCAGC 2 cut(s) 946, 1807
BstV2I GAAGAC 1 cut(s) 811
BstX2I RGATCY 3 cut(s) 817, 1526, 1990
BstYI RGATCY 3 cut(s) 817, 1526, 1990
BsuI GTATCC 1 cut(s) 1913
BsuRI GGCC 3 cut(s) 173, 1421, 1644
BtgI CCRYGG 3 cut(s) 75, 209, 963
BtgZI GCGATG 1 cut(s) 1529
BtrI CACGTC 1 cut(s) 374
BtsI GCAGTG 1 cut(s) 1692
BtsIMutI CAGTG 5 cut(s) 518, 609, 1233, 1692, 2065
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 1 cut(s) 206
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 2 cut(s) 206, 208
Cfr13I GGNCC 6 cut(s) 127, 1049, 1290, 1420, 1643, 1763
Cfr42I CCGCGG 1 cut(s) 78
CseI GACGC 2 cut(s) 289, 1342
Csp6I GTAC 2 cut(s) 26, 1973
CviAII CATG 7 cut(s) 149, 476, 977, 1319, 1368, 2032, 2066
CviQI GTAC 2 cut(s) 26, 1973
DpnI GATC 5 cut(s) 819, 1239, 1528, 1884, 1992
DpnII GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 3 cut(s) 611, 1059, 1087
EarI CTCTTC 3 cut(s) 611, 1059, 1087
EciI GGCGGA 3 cut(s) 58, 131, 287
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 948, 1596
Eco24I GRGCYC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 1723
Eco47I GGWCC 4 cut(s) 127, 1049, 1290, 1763
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 275, 1089
Eco72I CACGTG 2 cut(s) 35, 47
Eco88I CYCGRG 2 cut(s) 182, 1815
Eco91I GGTNACC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 139
EcoNI CCTNNNNNAGG 1 cut(s) 1788
EcoO109I RGGNCCY 3 cut(s) 127, 1049, 1290
EcoO65I GGTNACC 1 cut(s) 196
EcoRI GAATTC 2 cut(s) 1073, 1303
EcoT14I CCWWGG 2 cut(s) 948, 1596
EcoT22I ATGCAT 1 cut(s) 1628
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 948, 1596
Esp3I CGTCTC 1 cut(s) 1084
FaeI CATG 7 cut(s) 152, 479, 980, 1322, 1371, 2035, 2069
FalI AAGNNNNNCTT 4 cut(s) 320, 352, 385, 417
FaqI GGGAC 2 cut(s) 230, 332
FatI CATG 7 cut(s) 148, 475, 976, 1318, 1367, 2031, 2065
FauI CCCGC 1 cut(s) 185
FauNDI CATATG 1 cut(s) 541
FblI GTMKAC 2 cut(s) 177, 2007
Fnu4HI GCNGC 7 cut(s) 72, 75, 145, 156, 159, 960, 1821
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 7 cut(s) 72, 75, 145, 156, 159, 960, 1821
FspBI CTAG 4 cut(s) 866, 1379, 1952, 2106
GlaI GCGC 2 cut(s) 205, 207
GluI GCNGC 7 cut(s) 72, 75, 145, 156, 159, 960, 1821
GsaI CCCAGC 1 cut(s) 319
GsuI CTGGAG 2 cut(s) 275, 1329
HaeIII GGCC 3 cut(s) 173, 1421, 1644
HapII CCGG 1 cut(s) 17
HgaI GACGC 2 cut(s) 289, 1342
HhaI GCGC 2 cut(s) 206, 208
Hin1I GRCGYC 2 cut(s) 243, 300
Hin1II CATG 7 cut(s) 152, 479, 980, 1322, 1371, 2035, 2069
Hin6I GCGC 2 cut(s) 204, 206
HinP1I GCGC 2 cut(s) 204, 206
HincII GTYRAC 4 cut(s) 178, 857, 1090, 2008
HindII GTYRAC 4 cut(s) 178, 857, 1090, 2008
HpaI GTTAAC 1 cut(s) 1090
HpaII CCGG 1 cut(s) 17
HphI GGTGA 5 cut(s) 31, 190, 271, 928, 1848
Hpy188I TCNGA 8 cut(s) 803, 886, 1000, 1411, 1552, 1723, 2020, 2123
Hpy188III TCNNGA 8 cut(s) 184, 377, 498, 733, 1129, 1308, 1799, 2106
Hpy99I CGWCG 4 cut(s) 179, 1651, 1654, 2012
HpyCH4III ACNGT 1 cut(s) 1144
HpyCH4IV ACGT 7 cut(s) 34, 46, 102, 243, 373, 425, 532
HpyF10VI GCNNNNNNNGC 4 cut(s) 312, 1441, 1886, 2108
HpySE526I ACGT 7 cut(s) 34, 46, 102, 243, 373, 425, 532
Hsp92I GRCGYC 2 cut(s) 243, 300
Hsp92II CATG 7 cut(s) 152, 479, 980, 1322, 1371, 2035, 2069
HspAI GCGC 2 cut(s) 204, 206
KspAI GTTAAC 1 cut(s) 1090
KspI CCGCGG 1 cut(s) 78
Kzo9I GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
LmnI GCTCC 3 cut(s) 144, 294, 1310
Lsp1109I GCAGC 2 cut(s) 946, 1807
LweI GCATC 7 cut(s) 565, 871, 937, 1205, 1324, 1789, 1946
MaeI CTAG 4 cut(s) 866, 1379, 1952, 2106
MaeII ACGT 7 cut(s) 34, 46, 102, 243, 373, 425, 532
MaeIII GTNAC 5 cut(s) 42, 103, 196, 900, 1544
MalI GATC 5 cut(s) 819, 1239, 1528, 1884, 1992
MbiI CCGCTC 1 cut(s) 192
MboI GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
MboII GAAGA 9 cut(s) 358, 598, 634, 721, 816, 1046, 1074, 1082, 1174
MfeI CAATTG 2 cut(s) 429, 1250
MflI RGATCY 3 cut(s) 817, 1526, 1990
MhlI GDGCHC 4 cut(s) 52, 141, 291, 2065
MlyI GAGTC 4 cut(s) 212, 260, 314, 908
MmeI TCCRAC 2 cut(s) 1320, 1448
Mph1103I ATGCAT 1 cut(s) 1628
MroXI GAANNNNTTC 1 cut(s) 335
MseI TTAA 5 cut(s) 588, 1089, 1493, 1701, 2079
MslI CAYNNNNRTG 2 cut(s) 1201, 1674
MspA1I CMGCKG 2 cut(s) 77, 315
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 17
MunI CAATTG 2 cut(s) 429, 1250
Mva1269I GAATGC 4 cut(s) 1138, 1244, 1516, 1628
MvnI CGCG 2 cut(s) 77, 206
MwoI GCNNNNNNNGC 4 cut(s) 312, 1441, 1886, 2108
NciI CCSGG 1 cut(s) 17
NdeI CATATG 1 cut(s) 541
NdeII GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
NlaIII CATG 7 cut(s) 152, 479, 980, 1322, 1371, 2035, 2069
NlaIV GGNNCC 1 cut(s) 81
NmeAIII GCCGAG 2 cut(s) 160, 248
NmuCI GTSAC 4 cut(s) 42, 103, 196, 900
NsiI ATGCAT 1 cut(s) 1628
NspI RCATGY 2 cut(s) 1371, 2069
PaeR7I CTCGAG 1 cut(s) 182
PauI GCGCGC 1 cut(s) 204
PciI ACATGT 1 cut(s) 2065
PcsI WCGNNNNNNNCGW 4 cut(s) 159, 174, 1556, 2016
PctI GAATGC 4 cut(s) 1138, 1244, 1516, 1628
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 8 cut(s) 551, 690, 787, 881, 944, 1679, 2051, 2090
PflFI GACNNNGTC 2 cut(s) 424, 2117
PkrI GCNGC 7 cut(s) 73, 76, 146, 157, 160, 961, 1822
PleI GAGTC 4 cut(s) 212, 260, 314, 907
PmaCI CACGTG 2 cut(s) 35, 47
PmlI CACGTG 2 cut(s) 35, 47
PpsI GAGTC 4 cut(s) 212, 260, 314, 907
Ppu21I YACGTR 2 cut(s) 35, 47
PpuMI RGGWCCY 3 cut(s) 127, 1049, 1290
PscI ACATGT 1 cut(s) 2065
Psp124BI GAGCTC 1 cut(s) 141
Psp5II RGGWCCY 3 cut(s) 127, 1049, 1290
PspCI CACGTG 2 cut(s) 35, 47
PspEI GGTNACC 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 315
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 6 cut(s) 127, 1049, 1290, 1420, 1643, 1763
PspPPI RGGWCCY 3 cut(s) 127, 1049, 1290
PstI CTGCAG 1 cut(s) 1687
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 3 cut(s) 817, 1526, 1990
PsyI GACNNNGTC 2 cut(s) 424, 2117
PteI GCGCGC 1 cut(s) 204
RsaI GTAC 2 cut(s) 27, 1974
RsaNI GTAC 2 cut(s) 26, 1973
RseI CAYNNNNRTG 2 cut(s) 1201, 1674
SacI GAGCTC 1 cut(s) 141
SacII CCGCGG 1 cut(s) 78
SalI GTCGAC 2 cut(s) 176, 2006
SaqAI TTAA 5 cut(s) 588, 1089, 1493, 1701, 2079
SatI GCNGC 7 cut(s) 72, 75, 145, 156, 159, 960, 1821
Sau3AI GATC 5 cut(s) 817, 1237, 1526, 1882, 1990
Sau96I GGNCC 6 cut(s) 127, 1049, 1290, 1420, 1643, 1763
SchI GAGTC 4 cut(s) 212, 260, 314, 908
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 4 cut(s) 52, 141, 291, 2065
SfaNI GCATC 7 cut(s) 565, 871, 937, 1205, 1324, 1789, 1946
SfcI CTRYAG 1 cut(s) 1683
Sfr274I CTCGAG 1 cut(s) 182
Sfr303I CCGCGG 1 cut(s) 78
SgrBI CCGCGG 1 cut(s) 78
SinI GGWCC 4 cut(s) 127, 1049, 1290, 1763
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 1201, 1674
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SspMI CTAG 4 cut(s) 866, 1379, 1952, 2106
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 2 cut(s) 948, 1596
TaaI ACNGT 1 cut(s) 1144
TaiI ACGT 7 cut(s) 37, 49, 105, 246, 376, 428, 535
TaqI TCGA 7 cut(s) 120, 177, 183, 231, 390, 456, 2007
TatI WGTACW 1 cut(s) 1972
TauI GCSGC 5 cut(s) 74, 77, 147, 158, 161
TfiI GAWTC 8 cut(s) 551, 690, 787, 881, 944, 1679, 2051, 2090
Tru1I TTAA 5 cut(s) 588, 1089, 1493, 1701, 2079
Tru9I TTAA 5 cut(s) 588, 1089, 1493, 1701, 2079
TscAI CASTG 5 cut(s) 525, 616, 1240, 1692, 2065
TseFI GTSAC 4 cut(s) 42, 103, 196, 900
TseI GCWGC 2 cut(s) 959, 1820
Tsp45I GTSAC 4 cut(s) 42, 103, 196, 900
TspGWI ACGGA 2 cut(s) 631, 1171
TspRI CASTG 5 cut(s) 525, 616, 1240, 1692, 2065
Tth111I GACNNNGTC 2 cut(s) 424, 2117
VneI GTGCAC 1 cut(s) 2061
VpaK11BI GGWCC 4 cut(s) 127, 1049, 1290, 1763
XagI CCTNNNNNAGG 1 cut(s) 1788
XapI RAATTY 6 cut(s) 680, 847, 1040, 1073, 1303, 1845
XbaI TCTAGA 1 cut(s) 2105
XceI RCATGY 2 cut(s) 1371, 2069
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 2 cut(s) 177, 2007
XmnI GAANNNNTTC 1 cut(s) 335
XspI CTAG 4 cut(s) 866, 1379, 1952, 2106
ZraI GACGTC 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 1628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.