Rh5AG505000

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008402
Physical Location & Seq
Forward (+)
85482503 .. 85488146
5644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG505000.1

Sequence Viewer

Length: 2148 bp
ATGAACACTCACACTCACCGCCCGGTGAGGGCGCTCTCACGTGCCCTGTCACGTGCCTTATCCCAGTCTACTTCCTCTTCCTCCTTCCCTCTGAAAAACGTGACCAAATCGAACTTCGAGCCCGTTCTCGCCGAGCTCCGCCGCCACGTCGCCGCCGCCGACTTCGTGGCCGTCGACCTCGAAATGAGCGGAGTCACCAGCGCGCCGTGGCGGGACTCGTTGGAGTTCGACCGCTCTGACGTCAGCTACCTCAAGGTCAAGGACTCGGCGGAGAAGTTCGCCGTGCTTCAGTTCGGCGTTTGCCCCTTTCGCTGGGACTCCTCCAAGAAATCCTTCATTGCTCATCCGCACAATTTCTATGTTTTTCCACGTCAAGAGCTTCCTGTCAAAAGCCCCACTTATGAGTTTCTCTGCCAGACAACATCAATTGACTTCTTAGCTAAATACCAGTTCGATTTCAATATGTGTATACATGAAGGAATATCTTATTTATCTAGAGGGCAGGAAAATGAAGCACTGAGATGTTTTCATTTGGCATATGAGGATGGATCTAAGTTGAAAGAAGTAAGGGATGCCCCTTTGGTTAAGATGGCTGACACTCTCTTCACTGAACGGATGAAGAATACATTTACCGAGTGGCGTGATGGGTTGTTGAGAAATAAAAATGGAGGGTTCCAATTTCAGGGAATCTCAAATGACCGCCAGCAACATTTAGAAACCATTTTTTTCAAGATGCGTCCTGCTCTTAGTCTAAATGGATTTACATCTCATCAGCTTAAATTGATTCAACTGGTCATTAGAAGGCATTTCCAAGATCTTTCTTATGTTCAGGTAAATGGGGAAAATTCATGTCAACAGCAACTAGTTGTGCATACAGATTCGAAGGATGACAGGGAGTCACTTATGAAGGATGTGAAGGATGAGCATCTTAGACAAGCAGAGACCAAGGTTCAAGCTGCGGTGGGGTTTCGTCATGTTATTGACCTTCTTTCCTCAGAAAAAAAGCTGATTGTTGGTCACAATTGCTTTCTGGATATTGCACATATATACAGCAAATTTGTAGGTCCTCTTCCTTCAACTGCTGAAGAATTTGTATCTTCTGTTAACAAGTGTTTTCCGCACATCCTTGACACCAAAGTACTCTTGAATGCTAATATTGGGCTTCAACAACGGATGAAGAAATCCCAAACATCACTCTCGTCAGCATTTGCATCATTATGTCCACAAATTTCACTGGGGGCGAAAAGCACTGATCTGGCATTCCAATTGTGTGTCAAAGTTGAGGTTCAGGTTGATGATTTGAGGTCCTCCAACTGGAATTCTGGAGCTAAACATGAAGCTGGATATGATGCTTTTATGACAGGATGTGTCTTTGCTCAGGCCTGCAGTCATCTAGGTATTGATTTTGAAGCATGTTTGCCCATTGAAAAGTTGGCCCACAATGAAAAGCTCCAGAAGCACATCAACCTTCTATTCCTTAGTTGGAGTAAAGGAGATGTTATTGATTTAACAACTGGAAATAAGAATGCGATGTCTGTGGGATCTAAGAACAACAAAAAACGTTACTTTCAGATTTCATATGAGAAAATTGCTTTAATATGGGGATTTTCATCCAAGCTCAAGGCACGAGAGATAAGAGAATGCATCTCCAAAGTCTTTGGCCTGACCTCAGTTGTATCTATCTATCACTTGGATGAAACTGCAGTGTTTGTTCAGTTTAACAAGGAGAAATTGGTCTCTGAGTTTCTCAGTTTGAAGGACACTTTAGAGAGAAAAGATGGTCCTGTCTCGGTTTTGCATCCTCTTGCAGGACTTTTGGAAGGTGGAAATACCCGAGCTGCTGACTATGAAACTTACAAAGAAATTTGTAGTTCACCCAACGCAAAAGTCTTGTTTGCAGATCAGGCAGAGGCATTTGGTATCCAATGGAAAACCAAGTTGGTCGAAGCTAAGGCATCATTGTGCCTAGAATGTGAGAGTTCAGGTGAAAAAAACGCTTTGAATCCTGCTTCAAAATCCAATGAAAAATCTGAAAGAAAGAACATGGAAAATGTGCAGAGTGATTCATCTACTGCATGCGTCTCAAACTATAAGATTATAGATTCTTTGATTGCTTCTAGAGGCGATAAGGTCCGAACTAATTTGTGA

Protein Analysis

715

Amino Acids

80.46

Weight (kDa)

8.48

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 34 - 458 3.5e-102 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 243
AccBSI CCGCTC 2 cut(s) 189, 234
AccI GTMKAC 3 cut(s) 68, 174, 469
AccII CGCG 1 cut(s) 203
AclI AACGTT 1 cut(s) 1561
AclWI GGATC 2 cut(s) 556, 1549
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 6 cut(s) 844, 1055, 1088, 1227, 1318, 1863
AcuI CTGAAG 2 cut(s) 272, 1104
AcvI CACGTG 2 cut(s) 41, 53
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 1140
AfiI CCNNNNNNNGG 5 cut(s) 28, 312, 682, 1314, 1808
AhdI GACNNNNNGTC 1 cut(s) 895
AhlI ACTAGT 1 cut(s) 862
AjiI CACGTC 2 cut(s) 148, 371
AjuI GAANNNNNNNTTGG 6 cut(s) 98, 130, 317, 349, 1922, 1954
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 4 cut(s) 935, 1741, 1792, 2086
AlwI GGATC 2 cut(s) 556, 1549
Ama87I CYCGRG 1 cut(s) 1833
AoxI GGCC 4 cut(s) 168, 1380, 1434, 1660
ApeKI GCWGC 2 cut(s) 956, 1838
ApoI RAATTY 6 cut(s) 844, 1055, 1088, 1227, 1318, 1863
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 3 cut(s) 34, 203, 205
AspS9I GGNCC 5 cut(s) 1064, 1305, 1435, 1781, 2131
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 5 cut(s) 8, 37, 187, 1866, 1997
AsuII TTCGAA 1 cut(s) 881
AvaI CYCGRG 1 cut(s) 1833
AvaII GGWCC 4 cut(s) 1064, 1305, 1781, 2131
BaeGI GKGCMC 1 cut(s) 46
BanII GRGCYC 2 cut(s) 123, 138
BarI GAAGNNNNNNTAC 2 cut(s) 1053, 1085
BauI CACGAG 1 cut(s) 1626
BbrPI CACGTG 2 cut(s) 41, 53
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 943, 1825
BccI CCATC 4 cut(s) 539, 583, 638, 1772
BceAI ACGGC 3 cut(s) 155, 190, 266
BciVI GTATCC 1 cut(s) 1931
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 4 cut(s) 935, 1741, 1792, 2086
BcuI ACTAGT 1 cut(s) 862
BfaI CTAG 5 cut(s) 495, 863, 1394, 1967, 2118
BfmI CTRYAG 2 cut(s) 1384, 1701
BfoI RGCGCY 1 cut(s) 35
BfuI GTATCC 1 cut(s) 1931
BglII AGATCT 1 cut(s) 814
BisI GCNGC 5 cut(s) 142, 153, 156, 957, 1839
BlsI GCNGC 5 cut(s) 143, 154, 157, 958, 1840
BmcAI AGTACT 1 cut(s) 1140
Bme1390I CCNGG 1 cut(s) 23
Bme18I GGWCC 4 cut(s) 1064, 1305, 1781, 2131
BmeRI GACNNNNNGTC 1 cut(s) 895
BmeT110I CYCGRG 1 cut(s) 1833
BmgBI CACGTC 2 cut(s) 148, 371
BmgT120I GGNCC 5 cut(s) 1064, 1305, 1435, 1781, 2131
BmiI GGNNCC 1 cut(s) 674
BmrFI CCNGG 1 cut(s) 23
BmrI ACTGGG 2 cut(s) 58, 1244
BmsI GCATC 8 cut(s) 562, 723, 934, 1220, 1339, 1653, 1807, 1964
BmuI ACTGGG 2 cut(s) 58, 1244
BpmI CTGGAG 2 cut(s) 1344, 1436
Bpu10I CCTNAGC 2 cut(s) 1377, 1950
Bpu14I TTCGAA 1 cut(s) 881
BpuEI CTTGAG 2 cut(s) 236, 1604
BpuMI CCSGG 1 cut(s) 23
BsaAI YACGTR 2 cut(s) 41, 53
BsaBI GATNNNNATC 3 cut(s) 763, 924, 1609
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 2 cut(s) 935, 1741
BsaJI CCNNGG 2 cut(s) 206, 945
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 312, 682, 1314, 1808
Bse1I ACTGG 6 cut(s) 64, 448, 795, 1239, 1319, 1519
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 3 cut(s) 763, 924, 1609
BseDI CCNNGG 2 cut(s) 206, 945
BseJI GATNNNNATC 3 cut(s) 763, 924, 1609
BseLI CCNNNNNNNGG 5 cut(s) 28, 312, 682, 1314, 1808
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 6 cut(s) 509, 1008, 1391, 1683, 1731, 1762
BseNI ACTGG 6 cut(s) 64, 448, 795, 1239, 1319, 1519
BsePI GCGCGC 1 cut(s) 201
BseRI GAGGAG 1 cut(s) 310
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 2 cut(s) 943, 1825
BseYI CCCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 2075
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 4 cut(s) 170, 1382, 1436, 1662
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 1833
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 2 cut(s) 227, 329
BslI CCNNNNNNNGG 5 cut(s) 28, 312, 682, 1314, 1808
BsmAI GTCTC 4 cut(s) 935, 1741, 1792, 2086
BsmBI CGTCTC 1 cut(s) 2086
BsmFI GGGAC 2 cut(s) 227, 329
BsmI GAATGC 4 cut(s) 1153, 1259, 1531, 1646
BsnI GGCC 4 cut(s) 170, 1382, 1436, 1662
Bso31I GGTCTC 2 cut(s) 935, 1741
BsoBI CYCGRG 1 cut(s) 1833
Bsp119I TTCGAA 1 cut(s) 881
Bsp1286I GDGCHC 3 cut(s) 46, 123, 138
Bsp143I GATC 5 cut(s) 548, 814, 1252, 1541, 1900
BspANI GGCC 4 cut(s) 170, 1382, 1436, 1662
BspCNI CTCAG 6 cut(s) 510, 1007, 1390, 1682, 1732, 1761
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 1 cut(s) 674
BspMAI CTGCAG 2 cut(s) 1388, 1705
BspPI GGATC 2 cut(s) 556, 1549
BspT104I TTCGAA 1 cut(s) 881
BspTNI GGTCTC 2 cut(s) 935, 1741
BsrBI CCGCTC 2 cut(s) 189, 234
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 6 cut(s) 64, 448, 795, 1239, 1319, 1519
BssECI CCNNGG 2 cut(s) 206, 945
BssHII GCGCGC 1 cut(s) 201
BssMI GATC 5 cut(s) 548, 814, 1252, 1541, 1900
BssNAI GTATAC 1 cut(s) 470
BssNI GRCGYC 1 cut(s) 240
BssSI CACGAG 1 cut(s) 1626
BssT1I CCWWGG 1 cut(s) 945
Bst1107I GTATAC 1 cut(s) 470
Bst2BI CACGAG 1 cut(s) 1626
Bst6I CTCTTC 3 cut(s) 82, 608, 1074
BstACI GRCGYC 1 cut(s) 240
BstBAI YACGTR 2 cut(s) 41, 53
BstBI TTCGAA 1 cut(s) 881
BstC8I GCNNGC 4 cut(s) 203, 704, 1384, 2077
BstDSI CCRYGG 1 cut(s) 206
BstENI CCTNNNNNAGG 1 cut(s) 1806
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 3 cut(s) 34, 203, 205
BstKTI GATC 5 cut(s) 551, 817, 1255, 1544, 1903
BstMAI GTCTC 4 cut(s) 935, 1741, 1792, 2086
BstMBI GATC 5 cut(s) 548, 814, 1252, 1541, 1900
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 309, 1456, 1904
BstNSI RCATGY 2 cut(s) 1416, 2079
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 2 cut(s) 1384, 1701
BstSLI GKGCMC 1 cut(s) 46
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 943, 1825
BstX2I RGATCY 3 cut(s) 548, 814, 1541
BstYI RGATCY 3 cut(s) 548, 814, 1541
BstZ17I GTATAC 1 cut(s) 470
BsuI GTATCC 1 cut(s) 1931
BsuRI GGCC 4 cut(s) 170, 1382, 1436, 1662
BtgI CCRYGG 1 cut(s) 206
BtgZI GCGATG 1 cut(s) 1544
BtrI CACGTC 2 cut(s) 148, 371
BtsI GCAGTG 1 cut(s) 1710
BtsIMutI CAGTG 5 cut(s) 515, 606, 1232, 1248, 1710
Cac8I GCNNGC 4 cut(s) 203, 704, 1384, 2077
CfoI GCGC 3 cut(s) 34, 203, 205
Cfr13I GGNCC 5 cut(s) 1064, 1305, 1435, 1781, 2131
CseI GACGC 2 cut(s) 725, 2068
Csp6I GTAC 1 cut(s) 1139
CviAII CATG 7 cut(s) 473, 849, 974, 1334, 1413, 2044, 2076
CviQI GTAC 1 cut(s) 1139
DpnI GATC 5 cut(s) 550, 816, 1254, 1543, 1902
DpnII GATC 5 cut(s) 548, 814, 1252, 1541, 1900
DriI GACNNNNNGTC 1 cut(s) 895
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 3 cut(s) 82, 608, 1074
Eam1105I GACNNNNNGTC 1 cut(s) 895
EarI CTCTTC 3 cut(s) 82, 608, 1074
EciI GGCGGA 2 cut(s) 128, 284
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 945
Eco147I AGGCCT 1 cut(s) 1382
Eco24I GRGCYC 2 cut(s) 123, 138
Eco31I GGTCTC 2 cut(s) 935, 1741
Eco47I GGWCC 4 cut(s) 1064, 1305, 1781, 2131
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 2 cut(s) 272, 1104
Eco72I CACGTG 2 cut(s) 41, 53
Eco88I CYCGRG 1 cut(s) 1833
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 1806
EcoO109I RGGNCCY 2 cut(s) 1064, 1305
EcoRI GAATTC 1 cut(s) 1318
EcoT14I CCWWGG 1 cut(s) 945
EcoT22I ATGCAT 1 cut(s) 1646
EcoT38I GRGCYC 2 cut(s) 123, 138
ErhI CCWWGG 1 cut(s) 945
Esp3I CGTCTC 1 cut(s) 2086
FaeI CATG 7 cut(s) 476, 852, 977, 1337, 1416, 2047, 2079
FalI AAGNNNNNCTT 4 cut(s) 317, 349, 382, 414
FaqI GGGAC 2 cut(s) 227, 329
FatI CATG 7 cut(s) 472, 848, 973, 1333, 1412, 2043, 2075
FauI CCCGC 1 cut(s) 204
FauNDI CATATG 2 cut(s) 538, 1579
FblI GTMKAC 3 cut(s) 68, 174, 469
Fnu4HI GCNGC 5 cut(s) 142, 153, 156, 957, 1839
FriOI GRGCYC 2 cut(s) 123, 138
Fsp4HI GCNGC 5 cut(s) 142, 153, 156, 957, 1839
FspBI CTAG 5 cut(s) 495, 863, 1394, 1967, 2118
GlaI GCGC 3 cut(s) 33, 202, 204
GluI GCNGC 5 cut(s) 142, 153, 156, 957, 1839
GsaI CCCAGC 1 cut(s) 316
GsuI CTGGAG 2 cut(s) 1344, 1436
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 4 cut(s) 170, 1382, 1436, 1662
HapII CCGG 1 cut(s) 23
HgaI GACGC 2 cut(s) 725, 2068
HhaI GCGC 3 cut(s) 34, 203, 205
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 7 cut(s) 476, 852, 977, 1337, 1416, 2047, 2079
Hin6I GCGC 3 cut(s) 32, 201, 203
HinP1I GCGC 3 cut(s) 32, 201, 203
HincII GTYRAC 3 cut(s) 175, 854, 1105
HindII GTYRAC 3 cut(s) 175, 854, 1105
HpaI GTTAAC 1 cut(s) 1105
HpaII CCGG 1 cut(s) 23
HphI GGTGA 5 cut(s) 8, 37, 187, 1866, 1997
Hpy166II GTNNAC 7 cut(s) 69, 175, 470, 854, 1105, 1223, 1874
Hpy188I TCNGA 7 cut(s) 93, 238, 997, 1572, 1741, 2032, 2135
Hpy188III TCNNGA 8 cut(s) 374, 495, 730, 1031, 1144, 1323, 1453, 2118
Hpy8I GTNNAC 7 cut(s) 69, 175, 470, 854, 1105, 1223, 1874
Hpy99I CGWCG 2 cut(s) 152, 176
HpyCH4IV ACGT 7 cut(s) 40, 52, 99, 147, 240, 370, 1561
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 1456, 1904
HpySE526I ACGT 7 cut(s) 40, 52, 99, 147, 240, 370, 1561
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 7 cut(s) 476, 852, 977, 1337, 1416, 2047, 2079
HspAI GCGC 3 cut(s) 32, 201, 203
KspAI GTTAAC 1 cut(s) 1105
Kzo9I GATC 5 cut(s) 548, 814, 1252, 1541, 1900
LmnI GCTCC 3 cut(s) 141, 1325, 1455
Lsp1109I GCAGC 2 cut(s) 943, 1825
LweI GCATC 8 cut(s) 562, 723, 934, 1220, 1339, 1653, 1807, 1964
MaeI CTAG 5 cut(s) 495, 863, 1394, 1967, 2118
MaeII ACGT 7 cut(s) 40, 52, 99, 147, 240, 370, 1561
MaeIII GTNAC 6 cut(s) 48, 100, 193, 897, 1016, 1562
MalI GATC 5 cut(s) 550, 816, 1254, 1543, 1902
MbiI CCGCTC 2 cut(s) 189, 234
MboI GATC 5 cut(s) 548, 814, 1252, 1541, 1900
MboII GAAGA 7 cut(s) 69, 595, 631, 1061, 1089, 1097, 1189
MfeI CAATTG 3 cut(s) 426, 1021, 1265
MflI RGATCY 3 cut(s) 548, 814, 1541
MhlI GDGCHC 3 cut(s) 46, 123, 138
MlyI GAGTC 5 cut(s) 201, 209, 257, 311, 905
MmeI TCCRAC 3 cut(s) 201, 1335, 1463
Mph1103I ATGCAT 1 cut(s) 1646
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 6 cut(s) 585, 777, 1104, 1508, 1595, 1719
MslI CAYNNNNRTG 4 cut(s) 520, 1216, 1692, 1960
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 23
MunI CAATTG 3 cut(s) 426, 1021, 1265
Mva1269I GAATGC 4 cut(s) 1153, 1259, 1531, 1646
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 309, 1456, 1904
NciI CCSGG 1 cut(s) 23
NdeI CATATG 2 cut(s) 538, 1579
NdeII GATC 5 cut(s) 548, 814, 1252, 1541, 1900
NlaIII CATG 7 cut(s) 476, 852, 977, 1337, 1416, 2047, 2079
NlaIV GGNNCC 1 cut(s) 674
NmeAIII GCCGAG 2 cut(s) 157, 245
NmuCI GTSAC 5 cut(s) 48, 100, 193, 897, 1016
NsiI ATGCAT 1 cut(s) 1646
NspI RCATGY 2 cut(s) 1416, 2079
NspV TTCGAA 1 cut(s) 881
PaeI GCATGC 1 cut(s) 2079
PauI GCGCGC 1 cut(s) 201
PceI AGGCCT 1 cut(s) 1382
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 171
PctI GAATGC 4 cut(s) 1153, 1259, 1531, 1646
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 6 cut(s) 687, 784, 878, 2002, 2063, 2102
PkrI GCNGC 5 cut(s) 143, 154, 157, 958, 1840
PleI GAGTC 5 cut(s) 200, 209, 257, 311, 904
PmaCI CACGTG 2 cut(s) 41, 53
PmlI CACGTG 2 cut(s) 41, 53
PpsI GAGTC 5 cut(s) 200, 209, 257, 311, 904
Ppu21I YACGTR 2 cut(s) 41, 53
PpuMI RGGWCCY 2 cut(s) 1064, 1305
Psp124BI GAGCTC 1 cut(s) 138
Psp1406I AACGTT 1 cut(s) 1561
Psp5II RGGWCCY 2 cut(s) 1064, 1305
PspCI CACGTG 2 cut(s) 41, 53
PspFI CCCAGC 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 674
PspPI GGNCC 5 cut(s) 1064, 1305, 1435, 1781, 2131
PspPPI RGGWCCY 2 cut(s) 1064, 1305
PstI CTGCAG 2 cut(s) 1388, 1705
PsuI RGATCY 3 cut(s) 548, 814, 1541
PteI GCGCGC 1 cut(s) 201
RsaI GTAC 1 cut(s) 1140
RsaNI GTAC 1 cut(s) 1139
RseI CAYNNNNRTG 4 cut(s) 520, 1216, 1692, 1960
SacI GAGCTC 1 cut(s) 138
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 6 cut(s) 585, 777, 1104, 1508, 1595, 1719
SatI GCNGC 5 cut(s) 142, 153, 156, 957, 1839
Sau3AI GATC 5 cut(s) 548, 814, 1252, 1541, 1900
Sau96I GGNCC 5 cut(s) 1064, 1305, 1435, 1781, 2131
ScaI AGTACT 1 cut(s) 1140
SchI GAGTC 5 cut(s) 201, 209, 257, 311, 905
ScrFI CCNGG 1 cut(s) 23
SduI GDGCHC 3 cut(s) 46, 123, 138
SfaNI GCATC 8 cut(s) 562, 723, 934, 1220, 1339, 1653, 1807, 1964
SfcI CTRYAG 2 cut(s) 1384, 1701
SfuI TTCGAA 1 cut(s) 881
SinI GGWCC 4 cut(s) 1064, 1305, 1781, 2131
SmiMI CAYNNNNRTG 4 cut(s) 520, 1216, 1692, 1960
SmlI CTYRAG 2 cut(s) 251, 1619
SmoI CTYRAG 2 cut(s) 251, 1619
SpeI ACTAGT 1 cut(s) 862
SphI GCATGC 1 cut(s) 2079
SseBI AGGCCT 1 cut(s) 1382
SspI AATATT 1 cut(s) 1156
SspMI CTAG 5 cut(s) 495, 863, 1394, 1967, 2118
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 1382
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 1 cut(s) 945
TaiI ACGT 7 cut(s) 43, 55, 102, 150, 243, 373, 1564
TaqI TCGA 8 cut(s) 110, 117, 174, 180, 228, 453, 881, 1944
TatI WGTACW 1 cut(s) 1138
TauI GCSGC 3 cut(s) 144, 155, 158
TfiI GAWTC 6 cut(s) 687, 784, 878, 2002, 2063, 2102
Tru1I TTAA 6 cut(s) 585, 777, 1104, 1508, 1595, 1719
Tru9I TTAA 6 cut(s) 585, 777, 1104, 1508, 1595, 1719
TscAI CASTG 5 cut(s) 522, 613, 1239, 1255, 1710
TseFI GTSAC 5 cut(s) 48, 100, 193, 897, 1016
TseI GCWGC 2 cut(s) 956, 1838
Tsp45I GTSAC 5 cut(s) 48, 100, 193, 897, 1016
TspGWI ACGGA 2 cut(s) 628, 1186
TspRI CASTG 5 cut(s) 522, 613, 1239, 1255, 1710
VpaK11BI GGWCC 4 cut(s) 1064, 1305, 1781, 2131
XagI CCTNNNNNAGG 1 cut(s) 1806
XapI RAATTY 6 cut(s) 844, 1055, 1088, 1227, 1318, 1863
XbaI TCTAGA 2 cut(s) 494, 2117
XceI RCATGY 2 cut(s) 1416, 2079
XcmI CCANNNNNNNNNTGG 1 cut(s) 1429
XmiI GTMKAC 3 cut(s) 68, 174, 469
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 5 cut(s) 495, 863, 1394, 1967, 2118
ZraI GACGTC 1 cut(s) 241
ZrmI AGTACT 1 cut(s) 1140
Zsp2I ATGCAT 1 cut(s) 1646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.