RchiOBHm_Chr5g0077021

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008402
Physical Location & Seq
Forward (+)
82818333 .. 82823816
5484 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35165

Sequence Viewer

Length: 2139 bp
ATGAACACTCACCGCCCGGTGAGGGCGCTCTCACGTGCCCTGTCACGTGCGTTATCCCAGTCTACCTCCTCTTCCTCCTTCCCTCTGAAAAACGTGACCAAATCGAACTTCGAGCCGGTTCTCGCCGAGCTCCGCCGCCACGTCGCCGCCGCCGACTTCGTGGCCGTCGACCTCGAAATGAGCGGAGTCACCAGCGCGCCGTGGCGGGACTCCTTGGAGTTCGACCGCTCTGACGTCAGCTACCTCAAGGTCAAGGACTCGGCGGAGAAGTTCGCCGTGCTTCAGTTCGGCGTTTGCCCCTTTCGCTGGGACTCCTCCAAGAAATCCTTCATTGCTCATCCGCACAATTTCTATGTTTTTCCACGTCAAGAGCTTCCCGTCGAAAGCCCCACTTATGAGTTTCTCTGCCAGACTACATCAATTGACTTCTTAGCTAAATACCAGTTCGATTTCAATATGGGTATACATGAAGGGATATCTTATTTATCTAGAGGGCAGGAAAATGAAGCACTGAGATGTTTTCATTTGGCATATGAGGATGGATCTAAGTTGAAAGAAGTAAGGGATGCCCCTTTGGTTAAGATGGCTGACACTCTCTTCACTGAACGGATGAAGAATACATTTACCGAGTGGCGTGATGGGTTGTTGAGAAATAAAAATGGAGGGTTCCAATTTCAGGGAATCTCAAATGACCGCCAGCAACATTTAGAAACCATTTTTTTCAAGATGCGTCCTGCTCTTAGTCTAAATGGATTTACATCTCATCAACTTAAATTGATTCAACTGGTCATTAGAAGGCATTTCCAAGATCTTTCTTATGTTCAGGTAAATGGGGAAAATTCATGTCAACAGCGACTAGTTGTGCATACAGATTCGAAGGATGACAGGGAGTCACTTATGAAGGATGTGAAGGATGAGCATCTTAGACAAGCAGAGACCAAGGTTCAAGCTGCTGTGGGGTTTCGTCATGTTATTGACCTTCTTTCCTCAGAAAAAAAGCTGATTGTTGGTCACAATTGCTTTCTGGATATTGCACATATATACAGCAAATTTGTAGGTCCTCTTCCTTCAACTGCTGAAGAATTTGTATCTTCTGTTAACAAGTGTTTTCCGCACATCCTTGACACCAAAGTACTCTTGAATGCTAATATTGGGCTTCAACAACGGATGAAGAAATCCCAAACATCACTCTCGTCAGCATTTGCATCATTATGTCCACAAATTTCACTGGGGGCGAAAAGCACTGATCTGGCATTCCAATTGTGTGTCAAAGTTGAGGTTCAGGTTGATGATTTGAGGTCCTCCAACTGGAATTCTGGAGCCAAACATGAAGCTGGATATGATGCTTTTATGACAGGATGTGTCTTTGCTCAGGCCTGCAGTCATCTAGGTATTGATTTTGAAGCATGTTTGCCCTCTGAAAAGTTGGCCCACAATGAAAAGCTCCAGAAGCACATCAACCTTCTATTCCTTAGTTGGAGTAAAGGAGATGTTATTGATTTAACAACTGGAAATAAGAATGTGATGTCTGTGGGATCTAAGAACAACAAAAAACGTTACTTGCAGATTTCATATGAGAAAATTGCTTTAATATGGGGATTTTCAACCAAGCTCAAGGCACGAGAGATAAGAGAATGCATCTCCAAAGTCTTTGGCCTGACCTCAGTTGTATCTATCTATCACTTGGATGAAACTGCAGTGTTTGTTCAGTTTAACAAGGAGAAATTGGTCTCTGAGTTTCTCAGTTTGAAGGACACTTTAGAGAGAAAAGATGGTCCTGTCTCGGTTTTGCATCCCCTTGCAGGACTTTTGGAAGGTGGAAATACCCGAGCTGCTGATTATGAAACTTACAAAGAAATTTGTAGCTCACCCAACGCAAAAGTCTTGTTTGCAGATCAGGCAGAGGCATTTGGTATCCAATGGAAAACCAAGTTGGTCGAAGCTAAGGCATTGTGCCTAGAATGTGAGAGTTCAGGTGAAAAAAAAGCTTTGAATCCTGCTTCAAAATCCAATGAAAAATCCGAAAGAAAGAACATGGAAAATGTGCAGAGTGATTCATCTACTGCATGTGTCTCAAACTATAAGATTATAGATTCTTTGATTGCTTCTAGAGGCGATAAGGTCAGAACTAATTTGTGA

Protein Analysis

712

Amino Acids

80.11

Weight (kDa)

8.5

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 32 - 456 5.5e-101 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
AccBSI CCGCTC 2 cut(s) 183, 228
AccI GTMKAC 3 cut(s) 62, 168, 463
AccII CGCG 1 cut(s) 197
AclI AACGTT 1 cut(s) 1555
AclWI GGATC 2 cut(s) 550, 1543
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 6 cut(s) 838, 1049, 1082, 1221, 1312, 1857
AcuI CTGAAG 2 cut(s) 266, 1098
AcvI CACGTG 2 cut(s) 35, 47
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 1134
AfiI CCNNNNNNNGG 5 cut(s) 22, 306, 676, 1308, 1802
AhdI GACNNNNNGTC 1 cut(s) 889
AhlI ACTAGT 1 cut(s) 856
AjiI CACGTC 2 cut(s) 142, 365
AjuI GAANNNNNNNTTGG 6 cut(s) 92, 124, 311, 343, 1916, 1948
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 4 cut(s) 929, 1735, 1786, 2077
AlwI GGATC 2 cut(s) 550, 1543
Ama87I CYCGRG 1 cut(s) 1827
AoxI GGCC 4 cut(s) 162, 1374, 1428, 1654
ApeKI GCWGC 2 cut(s) 950, 1832
ApoI RAATTY 6 cut(s) 838, 1049, 1082, 1221, 1312, 1857
Asp700I GAANNNNTTC 1 cut(s) 326
AspLEI GCGC 3 cut(s) 28, 197, 199
AspS9I GGNCC 4 cut(s) 1058, 1299, 1429, 1775
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 4 cut(s) 31, 181, 1860, 1988
AsuII TTCGAA 1 cut(s) 875
AvaI CYCGRG 1 cut(s) 1827
AvaII GGWCC 3 cut(s) 1058, 1299, 1775
BaeGI GKGCMC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 1047, 1079
BauI CACGAG 1 cut(s) 1620
BbrPI CACGTG 2 cut(s) 35, 47
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 2 cut(s) 937, 1819
BccI CCATC 4 cut(s) 533, 577, 632, 1766
BceAI ACGGC 3 cut(s) 149, 184, 260
BciVI GTATCC 1 cut(s) 1925
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 4 cut(s) 929, 1735, 1786, 2077
BcuI ACTAGT 1 cut(s) 856
BfaI CTAG 5 cut(s) 489, 857, 1388, 1958, 2109
BfmI CTRYAG 2 cut(s) 1378, 1695
BfoI RGCGCY 1 cut(s) 29
BfuI GTATCC 1 cut(s) 1925
BglII AGATCT 1 cut(s) 808
BisI GCNGC 5 cut(s) 136, 147, 150, 951, 1833
BlsI GCNGC 5 cut(s) 137, 148, 151, 952, 1834
BmcAI AGTACT 1 cut(s) 1134
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 3 cut(s) 1058, 1299, 1775
BmeRI GACNNNNNGTC 1 cut(s) 889
BmeT110I CYCGRG 1 cut(s) 1827
BmgBI CACGTC 2 cut(s) 142, 365
BmgT120I GGNCC 4 cut(s) 1058, 1299, 1429, 1775
BmiI GGNNCC 2 cut(s) 668, 1321
BmrFI CCNGG 1 cut(s) 17
BmrI ACTGGG 2 cut(s) 52, 1238
BmsI GCATC 7 cut(s) 556, 717, 928, 1214, 1333, 1647, 1801
BmuI ACTGGG 2 cut(s) 52, 1238
BpmI CTGGAG 2 cut(s) 1338, 1430
Bpu10I CCTNAGC 2 cut(s) 1371, 1944
Bpu14I TTCGAA 1 cut(s) 875
BpuEI CTTGAG 2 cut(s) 230, 1598
BpuMI CCSGG 1 cut(s) 17
BsaAI YACGTR 2 cut(s) 35, 47
BsaBI GATNNNNATC 2 cut(s) 757, 918
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 2 cut(s) 929, 1735
BsaJI CCNNGG 3 cut(s) 200, 213, 939
Bsc4I CCNNNNNNNGG 5 cut(s) 22, 306, 676, 1308, 1802
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 6 cut(s) 58, 442, 789, 1233, 1313, 1513
Bse3DI GCAATG 1 cut(s) 330
Bse8I GATNNNNATC 2 cut(s) 757, 918
BseDI CCNNGG 3 cut(s) 200, 213, 939
BseJI GATNNNNATC 2 cut(s) 757, 918
BseLI CCNNNNNNNGG 5 cut(s) 22, 306, 676, 1308, 1802
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 6 cut(s) 503, 1002, 1385, 1677, 1725, 1756
BseNI ACTGG 6 cut(s) 58, 442, 789, 1233, 1313, 1513
BsePI GCGCGC 1 cut(s) 195
BseRI GAGGAG 2 cut(s) 58, 304
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 2 cut(s) 937, 1819
BseYI CCCAGC 1 cut(s) 306
BsgI GTGCAG 1 cut(s) 2066
Bsh1236I CGCG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 4 cut(s) 164, 1376, 1430, 1656
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 1827
BsiSI CCGG 2 cut(s) 17, 116
BslFI GGGAC 2 cut(s) 221, 323
BslI CCNNNNNNNGG 5 cut(s) 22, 306, 676, 1308, 1802
BsmAI GTCTC 4 cut(s) 929, 1735, 1786, 2077
BsmFI GGGAC 2 cut(s) 221, 323
BsmI GAATGC 3 cut(s) 1147, 1253, 1640
BsnI GGCC 4 cut(s) 164, 1376, 1430, 1656
Bso31I GGTCTC 2 cut(s) 929, 1735
BsoBI CYCGRG 1 cut(s) 1827
Bsp119I TTCGAA 1 cut(s) 875
Bsp1286I GDGCHC 2 cut(s) 40, 132
Bsp143I GATC 5 cut(s) 542, 808, 1246, 1535, 1894
BspANI GGCC 4 cut(s) 164, 1376, 1430, 1656
BspCNI CTCAG 6 cut(s) 504, 1001, 1384, 1676, 1726, 1755
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 2 cut(s) 668, 1321
BspMAI CTGCAG 2 cut(s) 1382, 1699
BspPI GGATC 2 cut(s) 550, 1543
BspT104I TTCGAA 1 cut(s) 875
BspTNI GGTCTC 2 cut(s) 929, 1735
BsrBI CCGCTC 2 cut(s) 183, 228
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 6 cut(s) 58, 442, 789, 1233, 1313, 1513
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 200, 213, 939
BssHII GCGCGC 1 cut(s) 195
BssMI GATC 5 cut(s) 542, 808, 1246, 1535, 1894
BssNAI GTATAC 1 cut(s) 464
BssNI GRCGYC 1 cut(s) 234
BssSI CACGAG 1 cut(s) 1620
BssT1I CCWWGG 2 cut(s) 213, 939
Bst1107I GTATAC 1 cut(s) 464
Bst2BI CACGAG 1 cut(s) 1620
Bst6I CTCTTC 3 cut(s) 76, 602, 1068
BstACI GRCGYC 1 cut(s) 234
BstBAI YACGTR 2 cut(s) 35, 47
BstBI TTCGAA 1 cut(s) 875
BstC8I GCNNGC 3 cut(s) 197, 698, 1378
BstDSI CCRYGG 1 cut(s) 200
BstFNI CGCG 1 cut(s) 197
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 3 cut(s) 28, 197, 199
BstKTI GATC 5 cut(s) 545, 811, 1249, 1538, 1897
BstMAI GTCTC 4 cut(s) 929, 1735, 1786, 2077
BstMBI GATC 5 cut(s) 542, 808, 1246, 1535, 1894
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 3 cut(s) 303, 1450, 1898
BstNSI RCATGY 2 cut(s) 1410, 2070
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 2 cut(s) 1378, 1695
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 937, 1819
BstX2I RGATCY 3 cut(s) 542, 808, 1535
BstYI RGATCY 3 cut(s) 542, 808, 1535
BstZ17I GTATAC 1 cut(s) 464
BsuI GTATCC 1 cut(s) 1925
BsuRI GGCC 4 cut(s) 164, 1376, 1430, 1656
BtgI CCRYGG 1 cut(s) 200
BtrI CACGTC 2 cut(s) 142, 365
BtsI GCAGTG 1 cut(s) 1704
BtsIMutI CAGTG 5 cut(s) 509, 600, 1226, 1242, 1704
Cac8I GCNNGC 3 cut(s) 197, 698, 1378
CfoI GCGC 3 cut(s) 28, 197, 199
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 4 cut(s) 1058, 1299, 1429, 1775
CseI GACGC 1 cut(s) 719
Csp6I GTAC 1 cut(s) 1133
CviAII CATG 7 cut(s) 467, 843, 968, 1328, 1407, 2035, 2067
CviQI GTAC 1 cut(s) 1133
DpnI GATC 5 cut(s) 544, 810, 1248, 1537, 1896
DpnII GATC 5 cut(s) 542, 808, 1246, 1535, 1894
DriI GACNNNNNGTC 1 cut(s) 889
EaeI YGGCCR 1 cut(s) 162
Eam1104I CTCTTC 3 cut(s) 76, 602, 1068
Eam1105I GACNNNNNGTC 1 cut(s) 889
EarI CTCTTC 3 cut(s) 76, 602, 1068
EciI GGCGGA 2 cut(s) 122, 278
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 213, 939
Eco147I AGGCCT 1 cut(s) 1376
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 2 cut(s) 929, 1735
Eco32I GATATC 1 cut(s) 477
Eco47I GGWCC 3 cut(s) 1058, 1299, 1775
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 2 cut(s) 266, 1098
Eco72I CACGTG 2 cut(s) 35, 47
Eco88I CYCGRG 1 cut(s) 1827
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 2 cut(s) 1058, 1299
EcoRI GAATTC 1 cut(s) 1312
EcoRV GATATC 1 cut(s) 477
EcoT14I CCWWGG 2 cut(s) 213, 939
EcoT22I ATGCAT 1 cut(s) 1640
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 213, 939
FaeI CATG 7 cut(s) 470, 846, 971, 1331, 1410, 2038, 2070
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 376, 408
FaqI GGGAC 2 cut(s) 221, 323
FatI CATG 7 cut(s) 466, 842, 967, 1327, 1406, 2034, 2066
FauI CCCGC 1 cut(s) 198
FauNDI CATATG 2 cut(s) 532, 1573
FblI GTMKAC 3 cut(s) 62, 168, 463
Fnu4HI GCNGC 5 cut(s) 136, 147, 150, 951, 1833
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 5 cut(s) 136, 147, 150, 951, 1833
FspBI CTAG 5 cut(s) 489, 857, 1388, 1958, 2109
GlaI GCGC 3 cut(s) 27, 196, 198
GluI GCNGC 5 cut(s) 136, 147, 150, 951, 1833
GsaI CCCAGC 1 cut(s) 310
GsuI CTGGAG 2 cut(s) 1338, 1430
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 4 cut(s) 164, 1376, 1430, 1656
HapII CCGG 2 cut(s) 17, 116
HgaI GACGC 1 cut(s) 719
HhaI GCGC 3 cut(s) 28, 197, 199
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 7 cut(s) 470, 846, 971, 1331, 1410, 2038, 2070
Hin6I GCGC 3 cut(s) 26, 195, 197
HinP1I GCGC 3 cut(s) 26, 195, 197
HincII GTYRAC 3 cut(s) 169, 848, 1099
HindII GTYRAC 3 cut(s) 169, 848, 1099
HindIII AAGCTT 1 cut(s) 1986
HpaI GTTAAC 1 cut(s) 1099
HpaII CCGG 2 cut(s) 17, 116
HphI GGTGA 4 cut(s) 31, 181, 1860, 1988
Hpy166II GTNNAC 6 cut(s) 63, 169, 464, 848, 1099, 1217
Hpy188I TCNGA 7 cut(s) 87, 232, 991, 1420, 1735, 2023, 2126
Hpy188III TCNNGA 8 cut(s) 368, 489, 724, 1025, 1138, 1317, 1447, 2109
Hpy8I GTNNAC 6 cut(s) 63, 169, 464, 848, 1099, 1217
Hpy99I CGWCG 3 cut(s) 146, 170, 383
HpyCH4IV ACGT 7 cut(s) 34, 46, 93, 141, 234, 364, 1555
HpyF10VI GCNNNNNNNGC 3 cut(s) 303, 1450, 1898
HpySE526I ACGT 7 cut(s) 34, 46, 93, 141, 234, 364, 1555
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 7 cut(s) 470, 846, 971, 1331, 1410, 2038, 2070
HspAI GCGC 3 cut(s) 26, 195, 197
KspAI GTTAAC 1 cut(s) 1099
Kzo9I GATC 5 cut(s) 542, 808, 1246, 1535, 1894
LmnI GCTCC 3 cut(s) 135, 1319, 1449
Lsp1109I GCAGC 2 cut(s) 937, 1819
LweI GCATC 7 cut(s) 556, 717, 928, 1214, 1333, 1647, 1801
MaeI CTAG 5 cut(s) 489, 857, 1388, 1958, 2109
MaeII ACGT 7 cut(s) 34, 46, 93, 141, 234, 364, 1555
MaeIII GTNAC 6 cut(s) 42, 94, 187, 891, 1010, 1556
MalI GATC 5 cut(s) 544, 810, 1248, 1537, 1896
MbiI CCGCTC 2 cut(s) 183, 228
MboI GATC 5 cut(s) 542, 808, 1246, 1535, 1894
MboII GAAGA 7 cut(s) 63, 589, 625, 1055, 1083, 1091, 1183
MfeI CAATTG 3 cut(s) 420, 1015, 1259
MflI RGATCY 3 cut(s) 542, 808, 1535
MhlI GDGCHC 2 cut(s) 40, 132
MlyI GAGTC 5 cut(s) 195, 203, 251, 305, 899
MmeI TCCRAC 2 cut(s) 1329, 1457
Mph1103I ATGCAT 1 cut(s) 1640
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 6 cut(s) 579, 771, 1098, 1502, 1589, 1713
MslI CAYNNNNRTG 3 cut(s) 514, 1210, 1686
MspI CCGG 2 cut(s) 17, 116
MspR9I CCNGG 1 cut(s) 17
MunI CAATTG 3 cut(s) 420, 1015, 1259
Mva1269I GAATGC 3 cut(s) 1147, 1253, 1640
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 303, 1450, 1898
NciI CCSGG 1 cut(s) 17
NdeI CATATG 2 cut(s) 532, 1573
NdeII GATC 5 cut(s) 542, 808, 1246, 1535, 1894
NlaIII CATG 7 cut(s) 470, 846, 971, 1331, 1410, 2038, 2070
NlaIV GGNNCC 2 cut(s) 668, 1321
NmeAIII GCCGAG 2 cut(s) 151, 239
NmuCI GTSAC 5 cut(s) 42, 94, 187, 891, 1010
NsiI ATGCAT 1 cut(s) 1640
NspI RCATGY 2 cut(s) 1410, 2070
NspV TTCGAA 1 cut(s) 875
PauI GCGCGC 1 cut(s) 195
PceI AGGCCT 1 cut(s) 1376
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 165
PctI GAATGC 3 cut(s) 1147, 1253, 1640
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 6 cut(s) 681, 778, 872, 1993, 2054, 2093
PkrI GCNGC 5 cut(s) 137, 148, 151, 952, 1834
PleI GAGTC 5 cut(s) 194, 203, 251, 305, 898
PmaCI CACGTG 2 cut(s) 35, 47
PmlI CACGTG 2 cut(s) 35, 47
PpsI GAGTC 5 cut(s) 194, 203, 251, 305, 898
Ppu21I YACGTR 2 cut(s) 35, 47
PpuMI RGGWCCY 2 cut(s) 1058, 1299
Psp124BI GAGCTC 1 cut(s) 132
Psp1406I AACGTT 1 cut(s) 1555
Psp5II RGGWCCY 2 cut(s) 1058, 1299
PspCI CACGTG 2 cut(s) 35, 47
PspFI CCCAGC 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 668, 1321
PspPI GGNCC 4 cut(s) 1058, 1299, 1429, 1775
PspPPI RGGWCCY 2 cut(s) 1058, 1299
PstI CTGCAG 2 cut(s) 1382, 1699
PsuI RGATCY 3 cut(s) 542, 808, 1535
PteI GCGCGC 1 cut(s) 195
RsaI GTAC 1 cut(s) 1134
RsaNI GTAC 1 cut(s) 1133
RseI CAYNNNNRTG 3 cut(s) 514, 1210, 1686
SacI GAGCTC 1 cut(s) 132
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 6 cut(s) 579, 771, 1098, 1502, 1589, 1713
SatI GCNGC 5 cut(s) 136, 147, 150, 951, 1833
Sau3AI GATC 5 cut(s) 542, 808, 1246, 1535, 1894
Sau96I GGNCC 4 cut(s) 1058, 1299, 1429, 1775
ScaI AGTACT 1 cut(s) 1134
SchI GAGTC 5 cut(s) 195, 203, 251, 305, 899
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 2 cut(s) 40, 132
SfaNI GCATC 7 cut(s) 556, 717, 928, 1214, 1333, 1647, 1801
SfcI CTRYAG 2 cut(s) 1378, 1695
SfuI TTCGAA 1 cut(s) 875
SinI GGWCC 3 cut(s) 1058, 1299, 1775
SmiMI CAYNNNNRTG 3 cut(s) 514, 1210, 1686
SmlI CTYRAG 2 cut(s) 245, 1613
SmoI CTYRAG 2 cut(s) 245, 1613
SpeI ACTAGT 1 cut(s) 856
SseBI AGGCCT 1 cut(s) 1376
SspI AATATT 1 cut(s) 1150
SspMI CTAG 5 cut(s) 489, 857, 1388, 1958, 2109
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 1376
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 2 cut(s) 213, 939
TaiI ACGT 7 cut(s) 37, 49, 96, 144, 237, 367, 1558
TaqI TCGA 9 cut(s) 104, 111, 168, 174, 222, 381, 447, 875, 1938
TatI WGTACW 1 cut(s) 1132
TauI GCSGC 3 cut(s) 138, 149, 152
TfiI GAWTC 6 cut(s) 681, 778, 872, 1993, 2054, 2093
Tru1I TTAA 6 cut(s) 579, 771, 1098, 1502, 1589, 1713
Tru9I TTAA 6 cut(s) 579, 771, 1098, 1502, 1589, 1713
TscAI CASTG 5 cut(s) 516, 607, 1233, 1249, 1704
TseFI GTSAC 5 cut(s) 42, 94, 187, 891, 1010
TseI GCWGC 2 cut(s) 950, 1832
Tsp45I GTSAC 5 cut(s) 42, 94, 187, 891, 1010
TspGWI ACGGA 2 cut(s) 622, 1180
TspRI CASTG 5 cut(s) 516, 607, 1233, 1249, 1704
VpaK11BI GGWCC 3 cut(s) 1058, 1299, 1775
XapI RAATTY 6 cut(s) 838, 1049, 1082, 1221, 1312, 1857
XbaI TCTAGA 2 cut(s) 488, 2108
XceI RCATGY 2 cut(s) 1410, 2070
XmiI GTMKAC 3 cut(s) 62, 168, 463
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 5 cut(s) 489, 857, 1388, 1958, 2109
ZraI GACGTC 1 cut(s) 235
ZrmI AGTACT 1 cut(s) 1134
Zsp2I ATGCAT 1 cut(s) 1640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.