Rh5BG526700

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008402
Physical Location & Seq
Forward (+)
82989065 .. 82994783
5719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG526700.1

Sequence Viewer

Length: 1557 bp
ATGGCTGACACTCTCTTCACTGAACGGATGAAGAATACATTTACCGAGTGGCGTGATGGGTTGTTGAGAAATAAAAATGGAGGGTTCCAATTTCAGGGAATCTCAAATGACCGCCAGCAACATTTAGAAACCATTTTTTTCAAGATGCGTCCTGCTCTTAGTCTAAATGGATTTACATCTCATCAGCTTAAATTGATTCAACTGGTCATTAGAAGGCATTTCCAAGATCTTTCTTATGTTCAGGTAAATGGGGAAAATTCATGTCAACAGCAACTAGTTGTGCATACAGATTCGAAGGATGACAGGGAGTCACTTATGAAGGATGTGAAGGATGAACATCTTAGACAAGCAGAGACCAAGGTTCAAGCTGCAGTGGGGTTTCGTCATGTTATTGACCTTCTTTCCTCAGAAAAAAAGCTGATTGTTGGTCACAATTGCTTTCTGGATATTGCACATATATACAGCAAATTTGTAGGTCCTCTTCCTTCAACTGCTGAAGAATTTGTATCTTCTGTTAACAAGTGTTTTCCGCACATCCTTGACACCAAAGTACTCTTGAATGCTAATATTGGGCTTCAACAACGGATGAAGAAATCCCAAACATCACTCTCGTCAGCATTTGCATCATTATGTCCACAAATTTCACTGGGGGCGAAAAGCACTGATCTGGCATTCCAATTGTGTGTCAAAGTTGAGGTTCAGGTTGATGATTTGAGGTCCTCCAACTGGAATTCTGGAGCCAAACATGAAGCTGGATATGATGCTTTTATGACAGGATGTGTCTTTGCCCAGGCCTGCAGTCATCTAGGTATTGATTTTGGAGCATGTTTGCCCTCTGAAAAGTTGGCCCACAATGAAAAGCTCCAGAAGCACATCAACCTTCTATTCCTTAGTTGGAGTAAAGGAGATGTTATTGATTTAACAACTGGAAATAAGAATGCGATGTCTGTGGGATCTAAGAACAACAAAAAACGTTACTTGCAGATTTCATATGAGAAAATTGCTTTAATATGGGGATTTTCATCCAAGCTCAAGGCACGAGAGATAAGAGAATGCATCTCCAAAGTCTTTGGCCTGACCTCAGTTGTATCTATCTATCACTTGGATGAAACTGCAGTGTTTGTTCAGTTTAACAAGGAGAAATTGGTCTCTGAGTTTCTCAGTTTGAAGGACACTTTAGAGAGAAAAGATGGTCCTGTCTCGGTTTTGCATCCTCTTGCAGGACTTTTGGAAGGTGGAAATACCCGAGCTGCTGATTATGAAACTTACAAAGAAATTTGTAGTTCACCCAATGCAAAAGTCTTGTTTGCAGATCAGGCAGAGGCATTTGGTATCCAATGGAAAACCAAGTTGGTCGAAGCTAAGGCATTGTGCCTAGAATGTGAGAGTTCAGGTGAAAAAAATGCTTTGAATCCTGCTTCAAAATCCAATGAAAAATCTGAAAGAAAGAACATGGAAAATGTGCAGAGTGATTCATCTACTGCATGTGTCTCAAACTATAAGATTATAGATTCTTTGATTGCCTCTAGAGCCGATATGGTCAGAACTAATTTGTGA

Protein Analysis

518

Amino Acids

57.91

Weight (kDa)

8.42

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 87 - 262 5.6e-45 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 112, 530
AclI AACGTT 1 cut(s) 973
AclWI GGATC 1 cut(s) 961
AcsI RAATTY 6 cut(s) 256, 467, 500, 639, 730, 1275
AcuI CTGAAG 1 cut(s) 516
AfaI GTAC 1 cut(s) 552
AfiI CCNNNNNNNGG 3 cut(s) 94, 726, 1220
AgsI TTSAA 9 cut(s) 142, 200, 365, 489, 559, 578, 1168, 1411, 1422
AhdI GACNNNNNGTC 1 cut(s) 307
AhlI ACTAGT 1 cut(s) 274
AjnI CCWGG 1 cut(s) 789
AjuI GAANNNNNNNTTGG 2 cut(s) 1334, 1366
AluBI AGCT 8 cut(s) 187, 368, 418, 752, 862, 1030, 1250, 1361
AluI AGCT 8 cut(s) 187, 368, 418, 752, 862, 1030, 1250, 1361
Alw26I GTCTC 4 cut(s) 347, 1153, 1204, 1495
AlwI GGATC 1 cut(s) 961
Ama87I CYCGRG 1 cut(s) 1245
AoxI GGCC 3 cut(s) 792, 846, 1072
ApeKI GCWGC 2 cut(s) 368, 1250
ApoI RAATTY 6 cut(s) 256, 467, 500, 639, 730, 1275
AspS9I GGNCC 4 cut(s) 476, 717, 847, 1193
AsuHPI GGTGA 2 cut(s) 1278, 1406
AsuII TTCGAA 1 cut(s) 293
AvaI CYCGRG 1 cut(s) 1245
AvaII GGWCC 3 cut(s) 476, 717, 1193
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BauI CACGAG 1 cut(s) 1038
BbvI GCAGC 2 cut(s) 355, 1237
BccI CCATC 2 cut(s) 50, 1184
BciT130I CCWGG 1 cut(s) 791
BciVI GTATCC 1 cut(s) 1343
BcoDI GTCTC 4 cut(s) 347, 1153, 1204, 1495
BcuI ACTAGT 1 cut(s) 274
BfaI CTAG 4 cut(s) 275, 806, 1376, 1527
BfmI CTRYAG 3 cut(s) 369, 796, 1113
BfuI GTATCC 1 cut(s) 1343
BglII AGATCT 1 cut(s) 226
BisI GCNGC 2 cut(s) 369, 1251
BlsI GCNGC 2 cut(s) 370, 1252
BmcAI AGTACT 1 cut(s) 552
Bme1390I CCNGG 1 cut(s) 791
Bme18I GGWCC 3 cut(s) 476, 717, 1193
BmeRI GACNNNNNGTC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 1245
BmgT120I GGNCC 4 cut(s) 476, 717, 847, 1193
BmiI GGNNCC 2 cut(s) 86, 739
BmrFI CCNGG 1 cut(s) 791
BmrI ACTGGG 1 cut(s) 656
BmsI GCATC 5 cut(s) 135, 632, 751, 1065, 1219
BmuI ACTGGG 1 cut(s) 656
BpmI CTGGAG 2 cut(s) 756, 848
Bpu10I CCTNAGC 1 cut(s) 1362
Bpu14I TTCGAA 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 1016
BsaBI GATNNNNATC 3 cut(s) 175, 336, 1021
BsaI GGTCTC 2 cut(s) 347, 1153
BsaJI CCNNGG 2 cut(s) 357, 789
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 726, 1220
Bse1I ACTGG 4 cut(s) 207, 651, 731, 931
Bse8I GATNNNNATC 3 cut(s) 175, 336, 1021
BseBI CCWGG 1 cut(s) 791
BseDI CCNNGG 2 cut(s) 357, 789
BseJI GATNNNNATC 3 cut(s) 175, 336, 1021
BseLI CCNNNNNNNGG 3 cut(s) 94, 726, 1220
BseMII CTCAG 4 cut(s) 420, 1095, 1143, 1174
BseNI ACTGG 4 cut(s) 207, 651, 731, 931
BseXI GCAGC 2 cut(s) 355, 1237
BsgI GTGCAG 1 cut(s) 1484
BshFI GGCC 3 cut(s) 794, 848, 1074
BsiHKCI CYCGRG 1 cut(s) 1245
BslI CCNNNNNNNGG 3 cut(s) 94, 726, 1220
BsmAI GTCTC 4 cut(s) 347, 1153, 1204, 1495
BsmI GAATGC 4 cut(s) 565, 671, 943, 1058
BsnI GGCC 3 cut(s) 794, 848, 1074
Bso31I GGTCTC 2 cut(s) 347, 1153
BsoBI CYCGRG 1 cut(s) 1245
Bsp119I TTCGAA 1 cut(s) 293
Bsp143I GATC 4 cut(s) 226, 664, 953, 1312
BspACI CCGC 2 cut(s) 112, 530
BspANI GGCC 3 cut(s) 794, 848, 1074
BspCNI CTCAG 4 cut(s) 419, 1094, 1144, 1173
BspLI GGNNCC 2 cut(s) 86, 739
BspMAI CTGCAG 3 cut(s) 373, 800, 1117
BspPI GGATC 1 cut(s) 961
BspT104I TTCGAA 1 cut(s) 293
BspTNI GGTCTC 2 cut(s) 347, 1153
BsrI ACTGG 4 cut(s) 207, 651, 731, 931
BssECI CCNNGG 2 cut(s) 357, 789
BssMI GATC 4 cut(s) 226, 664, 953, 1312
BssSI CACGAG 1 cut(s) 1038
BssT1I CCWWGG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 1038
Bst2UI CCWGG 1 cut(s) 791
Bst6I CTCTTC 2 cut(s) 20, 486
BstBI TTCGAA 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 116, 796
BstDEI CTNAG 9 cut(s) 158, 341, 406, 890, 957, 1081, 1152, 1160, 1362
BstENI CCTNNNNNAGG 1 cut(s) 1218
BstKTI GATC 4 cut(s) 229, 667, 956, 1315
BstMAI GTCTC 4 cut(s) 347, 1153, 1204, 1495
BstMBI GATC 4 cut(s) 226, 664, 953, 1312
BstMWI GCNNNNNNNGC 3 cut(s) 868, 1316, 1529
BstNI CCWGG 1 cut(s) 791
BstNSI RCATGY 2 cut(s) 828, 1488
BstSCI CCNGG 1 cut(s) 789
BstSFI CTRYAG 3 cut(s) 369, 796, 1113
BstV1I GCAGC 2 cut(s) 355, 1237
BstX2I RGATCY 2 cut(s) 226, 953
BstYI RGATCY 2 cut(s) 226, 953
BsuI GTATCC 1 cut(s) 1343
BsuRI GGCC 3 cut(s) 794, 848, 1074
BtgZI GCGATG 1 cut(s) 956
BtsI GCAGTG 2 cut(s) 378, 1122
BtsIMutI CAGTG 5 cut(s) 18, 378, 644, 660, 1122
Cac8I GCNNGC 2 cut(s) 116, 796
Cfr13I GGNCC 4 cut(s) 476, 717, 847, 1193
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 551
CviAII CATG 6 cut(s) 261, 386, 746, 825, 1453, 1485
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 9 cut(s) 158, 341, 406, 890, 957, 1081, 1152, 1160, 1362
DpnI GATC 4 cut(s) 228, 666, 955, 1314
DpnII GATC 4 cut(s) 226, 664, 953, 1312
DriI GACNNNNNGTC 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 20, 486
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 2 cut(s) 20, 486
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 794
Eco31I GGTCTC 2 cut(s) 347, 1153
Eco47I GGWCC 3 cut(s) 476, 717, 1193
Eco57I CTGAAG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 1245
EcoNI CCTNNNNNAGG 1 cut(s) 1218
EcoO109I RGGNCCY 2 cut(s) 476, 717
EcoRI GAATTC 1 cut(s) 730
EcoRII CCWGG 1 cut(s) 789
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 1058
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 6 cut(s) 264, 389, 749, 828, 1456, 1488
FatI CATG 6 cut(s) 260, 385, 745, 824, 1452, 1484
FauNDI CATATG 1 cut(s) 991
Fnu4HI GCNGC 2 cut(s) 369, 1251
Fsp4HI GCNGC 2 cut(s) 369, 1251
FspBI CTAG 4 cut(s) 275, 806, 1376, 1527
GluI GCNGC 2 cut(s) 369, 1251
GsuI CTGGAG 2 cut(s) 756, 848
HaeIII GGCC 3 cut(s) 794, 848, 1074
HgaI GACGC 1 cut(s) 137
Hin1II CATG 6 cut(s) 264, 389, 749, 828, 1456, 1488
HincII GTYRAC 2 cut(s) 266, 517
HindII GTYRAC 2 cut(s) 266, 517
HinfI GANTC 7 cut(s) 99, 196, 290, 308, 1411, 1472, 1511
HpaI GTTAAC 1 cut(s) 517
HphI GGTGA 2 cut(s) 1278, 1406
Hpy166II GTNNAC 4 cut(s) 266, 517, 635, 1286
Hpy188I TCNGA 5 cut(s) 409, 838, 1153, 1441, 1544
Hpy188III TCNNGA 6 cut(s) 142, 443, 556, 735, 865, 1527
Hpy8I GTNNAC 4 cut(s) 266, 517, 635, 1286
HpyAV CCTTC 9 cut(s) 207, 289, 313, 322, 407, 495, 890, 1162, 1226
HpyCH4IV ACGT 1 cut(s) 973
HpyF10VI GCNNNNNNNGC 3 cut(s) 868, 1316, 1529
HpyF3I CTNAG 9 cut(s) 158, 341, 406, 890, 957, 1081, 1152, 1160, 1362
HpySE526I ACGT 1 cut(s) 973
Hsp92II CATG 6 cut(s) 264, 389, 749, 828, 1456, 1488
KspAI GTTAAC 1 cut(s) 517
Kzo9I GATC 4 cut(s) 226, 664, 953, 1312
LmnI GCTCC 3 cut(s) 737, 821, 867
Lsp1109I GCAGC 2 cut(s) 355, 1237
LweI GCATC 5 cut(s) 135, 632, 751, 1065, 1219
MaeI CTAG 4 cut(s) 275, 806, 1376, 1527
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 3 cut(s) 309, 428, 974
MalI GATC 4 cut(s) 228, 666, 955, 1314
MboI GATC 4 cut(s) 226, 664, 953, 1312
MboII GAAGA 6 cut(s) 7, 43, 473, 501, 509, 601
MfeI CAATTG 2 cut(s) 433, 677
MflI RGATCY 2 cut(s) 226, 953
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 2 cut(s) 747, 875
Mph1103I ATGCAT 1 cut(s) 1058
MseI TTAA 5 cut(s) 189, 516, 920, 1007, 1131
MslI CAYNNNNRTG 2 cut(s) 628, 1104
MspR9I CCNGG 1 cut(s) 791
MunI CAATTG 2 cut(s) 433, 677
Mva1269I GAATGC 4 cut(s) 565, 671, 943, 1058
MvaI CCWGG 1 cut(s) 791
MwoI GCNNNNNNNGC 3 cut(s) 868, 1316, 1529
NdeI CATATG 1 cut(s) 991
NdeII GATC 4 cut(s) 226, 664, 953, 1312
NlaIII CATG 6 cut(s) 264, 389, 749, 828, 1456, 1488
NlaIV GGNNCC 2 cut(s) 86, 739
NmuCI GTSAC 2 cut(s) 309, 428
NsiI ATGCAT 1 cut(s) 1058
NspI RCATGY 2 cut(s) 828, 1488
NspV TTCGAA 1 cut(s) 293
PceI AGGCCT 1 cut(s) 794
PctI GAATGC 4 cut(s) 565, 671, 943, 1058
PfeI GAWTC 6 cut(s) 99, 196, 290, 1411, 1472, 1511
PkrI GCNGC 2 cut(s) 370, 1252
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 2 cut(s) 476, 717
Psp1406I AACGTT 1 cut(s) 973
Psp5II RGGWCCY 2 cut(s) 476, 717
Psp6I CCWGG 1 cut(s) 789
PspGI CCWGG 1 cut(s) 789
PspN4I GGNNCC 2 cut(s) 86, 739
PspPI GGNCC 4 cut(s) 476, 717, 847, 1193
PspPPI RGGWCCY 2 cut(s) 476, 717
PstI CTGCAG 3 cut(s) 373, 800, 1117
PsuI RGATCY 2 cut(s) 226, 953
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
RseI CAYNNNNRTG 2 cut(s) 628, 1104
SaqAI TTAA 5 cut(s) 189, 516, 920, 1007, 1131
SatI GCNGC 2 cut(s) 369, 1251
Sau3AI GATC 4 cut(s) 226, 664, 953, 1312
Sau96I GGNCC 4 cut(s) 476, 717, 847, 1193
ScaI AGTACT 1 cut(s) 552
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 791
SfaNI GCATC 5 cut(s) 135, 632, 751, 1065, 1219
SfcI CTRYAG 3 cut(s) 369, 796, 1113
SfuI TTCGAA 1 cut(s) 293
SinI GGWCC 3 cut(s) 476, 717, 1193
SmiMI CAYNNNNRTG 2 cut(s) 628, 1104
SmlI CTYRAG 1 cut(s) 1031
SmoI CTYRAG 1 cut(s) 1031
SpeI ACTAGT 1 cut(s) 274
SseBI AGGCCT 1 cut(s) 794
SsiI CCGC 2 cut(s) 112, 530
SspI AATATT 1 cut(s) 568
SspMI CTAG 4 cut(s) 275, 806, 1376, 1527
StuI AGGCCT 1 cut(s) 794
StyD4I CCNGG 1 cut(s) 789
StyI CCWWGG 1 cut(s) 357
TaiI ACGT 1 cut(s) 976
TaqI TCGA 2 cut(s) 293, 1356
TatI WGTACW 1 cut(s) 550
TfiI GAWTC 6 cut(s) 99, 196, 290, 1411, 1472, 1511
Tru1I TTAA 5 cut(s) 189, 516, 920, 1007, 1131
Tru9I TTAA 5 cut(s) 189, 516, 920, 1007, 1131
TscAI CASTG 5 cut(s) 25, 378, 651, 667, 1122
TseFI GTSAC 2 cut(s) 309, 428
TseI GCWGC 2 cut(s) 368, 1250
Tsp45I GTSAC 2 cut(s) 309, 428
TspGWI ACGGA 2 cut(s) 40, 598
TspRI CASTG 5 cut(s) 25, 378, 651, 667, 1122
VpaK11BI GGWCC 3 cut(s) 476, 717, 1193
XagI CCTNNNNNAGG 1 cut(s) 1218
XapI RAATTY 6 cut(s) 256, 467, 500, 639, 730, 1275
XbaI TCTAGA 1 cut(s) 1526
XceI RCATGY 2 cut(s) 828, 1488
XspI CTAG 4 cut(s) 275, 806, 1376, 1527
ZrmI AGTACT 1 cut(s) 552
Zsp2I ATGCAT 1 cut(s) 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.