MD03G1026800.v1.1

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008402
Physical Location & Seq
Reverse (-)
2158145 .. 2160203
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1026800.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGGCTGACACTCTCTTCGCTGAACGGATGAAGAACTCATTCACTGAATGGCGTGATGGTTTGTTAAGAAGCAGTAAGGGAGGATTCCAGTTTCAGGGAGACTCAAATGAAGGTCAATTTGATTTTATGACTAATATGTCTTCTTCTTGTCATGCAGATATTGCCCATGTATACAGCAAATTCCTGGGCCCTCTTCCTTCGACTGCTGAAGAATTTGTCTCAACTGTTAACAAGTACTTTCCACACGTCATTGACACCAAAGTACTATTGAACACGGATGATGTGCTCCAACAACGGATGAAGAAATCCCGAACATCACTTTCATCAGCATTTGCTTTGTTATGCCTACAAATTGCTCTTGGGAAGAAAAGCACTGATTCGGAAGTCCAAATGTGTGTCAACGTTGAAGTTCAGGTGGATGATTTGAGGTCCTCCAACTGGAGTTCTGGAGCCAAACATGAAGCGGGATATGATGCTTTTATGACAGGCTGCGTCTTTGCTCAGACATGCAGTCATCTCGGAATTGATTTTCAGGCCTGTTCGTCATCTGAAAAGTTGGCCCACAACGAGAAGCTCCAGAAGCAGATCAACCATCTATACCTCAGTTGGAGTAATGGAGACATTATTGATTTAACCACTGGTAAGAAGAATGCAATGTCTTCGGGGTCCAACAACCACAAAAAGAGGTACTCGCAGATTATGTCTGAGAACATTGCTTTAATCTGGGGATTCCCATCAAAACTCAAGGCACGAGACATCAGGGAGTGCATTTCAAAAGTCTTTGGCCCAACCTCTGTTACGTCTGTCTTCCACTTGGATGAAACTGCGGTATTCGTTCAGTTTAGCAAGGAAAAATTTGTGTCTGAATTTCTGGCTTTGAAGGAAACATTAGAGAGAAGTGATGGTCCAGTCTCAGTTTTGCATCCCCTCGCGGGACTTTTGGAAGGTGGAAGCACCCGAGCTGCTAACTATGAAACTTACAAGGAAATCTGCAGTTCACCCAGCGCAAAAGGCTTTTTTGCGGATCAGGCTGAGGCAGTTGGTATTAAATGGAAAACTAAATTGGTTGAATCCAAGGAAGCATCAGAGACCCTAAAACGGGAGAGCTTCGATGAAAAAAGTGAAGTGAATCCTGCTTCGAAGACCGAAAAATTGAGAAGAAGTACCACAGACACTGCAGAAAATGATCCGTTCTGTGGGCGTCGCTCACAATACAAGATGATAGATACTTTAATTGCTTCTGAAGGCGATCGAATGAGAACTAATTAG

Protein Analysis

423

Amino Acids

47.02

Weight (kDa)

6.57

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 50 - 166 1.5e-26 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 171
AccII CGCG 1 cut(s) 932
AciI CCGC 4 cut(s) 464, 827, 932, 1022
AclI AACGTT 1 cut(s) 402
AclWI GGATC 2 cut(s) 1032, 1181
AcsI RAATTY 4 cut(s) 179, 212, 854, 866
AcuI CTGAAG 2 cut(s) 228, 1263
AcyI GRCGYC 1 cut(s) 1201
AfaI GTAC 4 cut(s) 236, 264, 689, 1165
AfiI CCNNNNNNNGG 6 cut(s) 94, 438, 932, 1098, 1099, 1196
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 5 cut(s) 271, 407, 774, 880, 1070
AhdI GACNNNNNGTC 1 cut(s) 510
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 1 cut(s) 183
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AluBI AGCT 3 cut(s) 574, 962, 1107
AluI AGCT 3 cut(s) 574, 962, 1107
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 6 cut(s) 93, 223, 612, 747, 916, 1082
AlwI GGATC 2 cut(s) 1032, 1181
AlwNI CAGNNNCTG 1 cut(s) 1175
Ama87I CYCGRG 1 cut(s) 957
AoxI GGCC 4 cut(s) 187, 534, 558, 784
ApaI GGGCCC 1 cut(s) 191
ApeKI GCWGC 2 cut(s) 489, 962
ApoI RAATTY 4 cut(s) 179, 212, 854, 866
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 1 cut(s) 1007
AspS9I GGNCC 7 cut(s) 187, 188, 429, 559, 666, 785, 905
AsuHPI GGTGA 1 cut(s) 990
AsuII TTCGAA 1 cut(s) 1139
AvaI CYCGRG 1 cut(s) 957
AvaII GGWCC 3 cut(s) 429, 666, 905
BaeGI GKGCMC 1 cut(s) 191
BanII GRGCYC 1 cut(s) 191
BauI CACGAG 1 cut(s) 750
BbsI GAAGAC 4 cut(s) 132, 651, 799, 1148
Bbv12I GWGCWC 1 cut(s) 288
BbvCI CCTCAGC 1 cut(s) 1032
BbvI GCAGC 2 cut(s) 476, 949
BccI CCATC 4 cut(s) 50, 600, 742, 896
BcgI CGANNNNNNTGC 4 cut(s) 499, 533, 642, 676
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 6 cut(s) 93, 223, 612, 747, 916, 1082
BfmI CTRYAG 2 cut(s) 991, 1176
BisI GCNGC 2 cut(s) 490, 963
BlsI GCNGC 2 cut(s) 491, 964
BmcAI AGTACT 2 cut(s) 236, 264
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 3 cut(s) 429, 666, 905
BmeRI GACNNNNNGTC 1 cut(s) 510
BmeT110I CYCGRG 1 cut(s) 957
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 7 cut(s) 187, 188, 429, 559, 666, 785, 905
BmiI GGNNCC 3 cut(s) 189, 451, 667
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 3 cut(s) 463, 931, 1091
BpiI GAAGAC 4 cut(s) 132, 651, 799, 1148
BpmI CTGGAG 3 cut(s) 460, 468, 560
Bpu10I CCTNAGC 1 cut(s) 1032
Bpu14I TTCGAA 1 cut(s) 1139
BpuEI CTTGAG 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 733
BsaHI GRCGYC 1 cut(s) 1201
BsaI GGTCTC 1 cut(s) 1082
BsaJI CCNNGG 2 cut(s) 184, 1074
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 438, 932, 1098, 1099, 1196
Bse1I ACTGG 4 cut(s) 88, 443, 643, 908
Bse3DI GCAATG 2 cut(s) 660, 711
Bse8I GATNNNNATC 1 cut(s) 733
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 2 cut(s) 184, 1074
BseGI GGATG 6 cut(s) 33, 283, 303, 424, 823, 922
BseJI GATNNNNATC 1 cut(s) 733
BseLI CCNNNNNNNGG 6 cut(s) 94, 438, 932, 1098, 1099, 1196
BseMI GCAATG 2 cut(s) 660, 711
BseMII CTCAG 5 cut(s) 515, 616, 696, 927, 1023
BseNI ACTGG 4 cut(s) 88, 443, 643, 908
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 2 cut(s) 476, 949
BseYI CCCAGC 1 cut(s) 1001
Bsh1236I CGCG 1 cut(s) 932
Bsh1285I CGRYCG 1 cut(s) 1252
BshFI GGCC 4 cut(s) 189, 536, 560, 786
BsiEI CGRYCG 1 cut(s) 1252
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 957
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 6 cut(s) 94, 438, 932, 1098, 1099, 1196
BsmAI GTCTC 6 cut(s) 93, 223, 612, 747, 916, 1082
BsmFI GGGAC 1 cut(s) 948
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 4 cut(s) 189, 536, 560, 786
Bso31I GGTCTC 1 cut(s) 1082
BsoBI CYCGRG 1 cut(s) 957
Bsp119I TTCGAA 1 cut(s) 1139
Bsp120I GGGCCC 1 cut(s) 187
Bsp1286I GDGCHC 2 cut(s) 191, 288
Bsp143I GATC 4 cut(s) 585, 1024, 1186, 1249
BspACI CCGC 4 cut(s) 464, 827, 932, 1022
BspANI GGCC 4 cut(s) 189, 536, 560, 786
BspCNI CTCAG 5 cut(s) 514, 615, 697, 926, 1024
BspFNI CGCG 1 cut(s) 932
BspLI GGNNCC 3 cut(s) 189, 451, 667
BspMAI CTGCAG 2 cut(s) 995, 1180
BspPI GGATC 2 cut(s) 1032, 1181
BspT104I TTCGAA 1 cut(s) 1139
BspTNI GGTCTC 1 cut(s) 1082
BsrDI GCAATG 2 cut(s) 660, 711
BsrI ACTGG 4 cut(s) 88, 443, 643, 908
BssECI CCNNGG 2 cut(s) 184, 1074
BssMI GATC 4 cut(s) 585, 1024, 1186, 1249
BssNAI GTATAC 1 cut(s) 172
BssNI GRCGYC 1 cut(s) 1201
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 1 cut(s) 1074
Bst1107I GTATAC 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 2 cut(s) 20, 198
BstACI GRCGYC 1 cut(s) 1201
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 1139
BstDEI CTNAG 5 cut(s) 501, 602, 705, 913, 1032
BstF5I GGATG 6 cut(s) 33, 283, 303, 424, 823, 922
BstFNI CGCG 1 cut(s) 932
BstHHI GCGC 1 cut(s) 1007
BstKTI GATC 4 cut(s) 588, 1027, 1189, 1252
BstMAI GTCTC 6 cut(s) 93, 223, 612, 747, 916, 1082
BstMBI GATC 4 cut(s) 585, 1024, 1186, 1249
BstMCI CGRYCG 1 cut(s) 1252
BstMWI GCNNNNNNNGC 4 cut(s) 161, 580, 1011, 1028
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 2 cut(s) 991, 1176
BstSLI GKGCMC 1 cut(s) 191
BstUI CGCG 1 cut(s) 932
BstV1I GCAGC 2 cut(s) 476, 949
BstV2I GAAGAC 4 cut(s) 132, 651, 799, 1148
BstZ17I GTATAC 1 cut(s) 172
BsuRI GGCC 4 cut(s) 189, 536, 560, 786
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 6 cut(s) 33, 283, 303, 424, 823, 922
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 4 cut(s) 42, 372, 636, 1173
CaiI CAGNNNCTG 1 cut(s) 1175
CfoI GCGC 1 cut(s) 1007
Cfr13I GGNCC 7 cut(s) 187, 188, 429, 559, 666, 785, 905
CseI GACGC 2 cut(s) 481, 1190
Csp6I GTAC 4 cut(s) 235, 263, 688, 1164
CviAII CATG 4 cut(s) 152, 167, 458, 507
CviQI GTAC 4 cut(s) 235, 263, 688, 1164
DdeI CTNAG 5 cut(s) 501, 602, 705, 913, 1032
DpnI GATC 4 cut(s) 587, 1026, 1188, 1251
DpnII GATC 4 cut(s) 585, 1024, 1186, 1249
DrdI GACNNNNNNGTC 1 cut(s) 136
DriI GACNNNNNGTC 1 cut(s) 510
DseDI GACNNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 20, 198
Eam1105I GACNNNNNGTC 1 cut(s) 510
EarI CTCTTC 2 cut(s) 20, 198
Eco130I CCWWGG 1 cut(s) 1074
Eco147I AGGCCT 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 1082
Eco47I GGWCC 3 cut(s) 429, 666, 905
Eco57I CTGAAG 2 cut(s) 228, 1263
Eco88I CYCGRG 1 cut(s) 957
EcoO109I RGGNCCY 2 cut(s) 188, 429
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 1074
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 1074
FaeI CATG 4 cut(s) 155, 170, 461, 510
FaqI GGGAC 1 cut(s) 948
FatI CATG 4 cut(s) 151, 166, 457, 506
FauI CCCGC 2 cut(s) 457, 925
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 490, 963
FokI GGATG 6 cut(s) 40, 290, 310, 431, 830, 909
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 490, 963
GlaI GCGC 1 cut(s) 1006
GluI GCNGC 2 cut(s) 490, 963
GsaI CCCAGC 1 cut(s) 1005
GsuI CTGGAG 3 cut(s) 460, 468, 560
HaeIII GGCC 4 cut(s) 189, 536, 560, 786
HgaI GACGC 2 cut(s) 481, 1190
HhaI GCGC 1 cut(s) 1007
Hin1I GRCGYC 1 cut(s) 1201
Hin1II CATG 4 cut(s) 155, 170, 461, 510
Hin6I GCGC 1 cut(s) 1005
HinP1I GCGC 1 cut(s) 1005
HincII GTYRAC 2 cut(s) 229, 400
HindII GTYRAC 2 cut(s) 229, 400
HinfI GANTC 6 cut(s) 84, 101, 377, 729, 1070, 1129
HpaI GTTAAC 1 cut(s) 229
HphI GGTGA 1 cut(s) 990
Hpy166II GTNNAC 4 cut(s) 172, 229, 400, 998
Hpy188I TCNGA 8 cut(s) 382, 504, 521, 550, 706, 865, 1087, 1243
Hpy188III TCNNGA 3 cut(s) 309, 447, 577
Hpy8I GTNNAC 4 cut(s) 172, 229, 400, 998
Hpy99I CGWCG 1 cut(s) 1206
HpyAV CCTTC 5 cut(s) 104, 207, 874, 938, 1238
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4IV ACGT 3 cut(s) 246, 402, 800
HpyCH4V TGCA 7 cut(s) 155, 510, 653, 768, 922, 993, 1178
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 580, 1011, 1028
HpyF3I CTNAG 5 cut(s) 501, 602, 705, 913, 1032
HpySE526I ACGT 3 cut(s) 246, 402, 800
Hsp92I GRCGYC 1 cut(s) 1201
Hsp92II CATG 4 cut(s) 155, 170, 461, 510
HspAI GCGC 1 cut(s) 1005
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 4 cut(s) 585, 1024, 1186, 1249
LmnI GCTCC 3 cut(s) 291, 449, 579
Lsp1109I GCAGC 2 cut(s) 476, 949
LweI GCATC 3 cut(s) 463, 931, 1091
MaeII ACGT 3 cut(s) 246, 402, 800
MaeIII GTNAC 1 cut(s) 796
MalI GATC 4 cut(s) 587, 1026, 1188, 1251
MboI GATC 4 cut(s) 585, 1024, 1186, 1249
MhlI GDGCHC 2 cut(s) 191, 288
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 4 cut(s) 313, 459, 587, 693
MnlI CCTC 9 cut(s) 74, 201, 420, 442, 611, 678, 802, 938, 1027
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 6 cut(s) 65, 228, 632, 719, 1047, 1232
MslI CAYNNNNRTG 1 cut(s) 816
MspR9I CCNGG 1 cut(s) 185
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 932
MwoI GCNNNNNNNGC 4 cut(s) 161, 580, 1011, 1028
NdeII GATC 4 cut(s) 585, 1024, 1186, 1249
NlaIII CATG 4 cut(s) 155, 170, 461, 510
NlaIV GGNNCC 3 cut(s) 189, 451, 667
NspI RCATGY 1 cut(s) 510
NspV TTCGAA 1 cut(s) 1139
PceI AGGCCT 1 cut(s) 536
PctI GAATGC 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 5 cut(s) 84, 377, 729, 1070, 1129
PkrI GCNGC 2 cut(s) 491, 964
Ple19I CGATCG 1 cut(s) 1252
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 429
Psp1406I AACGTT 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 1001
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 189, 451, 667
PspOMI GGGCCC 1 cut(s) 187
PspPI GGNCC 7 cut(s) 187, 188, 429, 559, 666, 785, 905
PspPPI RGGWCCY 1 cut(s) 429
PstI CTGCAG 2 cut(s) 995, 1180
PstNI CAGNNNCTG 1 cut(s) 1175
PvuI CGATCG 1 cut(s) 1252
RsaI GTAC 4 cut(s) 236, 264, 689, 1165
RsaNI GTAC 4 cut(s) 235, 263, 688, 1164
RseI CAYNNNNRTG 1 cut(s) 816
SaqAI TTAA 6 cut(s) 65, 228, 632, 719, 1047, 1232
SatI GCNGC 2 cut(s) 490, 963
Sau3AI GATC 4 cut(s) 585, 1024, 1186, 1249
Sau96I GGNCC 7 cut(s) 187, 188, 429, 559, 666, 785, 905
ScaI AGTACT 2 cut(s) 236, 264
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 2 cut(s) 191, 288
SfaNI GCATC 3 cut(s) 463, 931, 1091
SfcI CTRYAG 2 cut(s) 991, 1176
SfuI TTCGAA 1 cut(s) 1139
SinI GGWCC 3 cut(s) 429, 666, 905
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 4 cut(s) 464, 827, 932, 1022
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 1 cut(s) 226
TaiI ACGT 3 cut(s) 249, 405, 803
TaqI TCGA 4 cut(s) 200, 1110, 1139, 1252
TaqII GACCGA 1 cut(s) 1160
TatI WGTACW 2 cut(s) 234, 262
TfiI GAWTC 5 cut(s) 84, 377, 729, 1070, 1129
Tru1I TTAA 6 cut(s) 65, 228, 632, 719, 1047, 1232
Tru9I TTAA 6 cut(s) 65, 228, 632, 719, 1047, 1232
TscAI CASTG 4 cut(s) 49, 379, 643, 1180
TseI GCWGC 2 cut(s) 489, 962
TspDTI ATGAA 8 cut(s) 44, 123, 312, 314, 474, 834, 987, 1128
TspGWI ACGGA 4 cut(s) 40, 290, 310, 1179
TspRI CASTG 4 cut(s) 49, 379, 643, 1180
VpaK11BI GGWCC 3 cut(s) 429, 666, 905
XapI RAATTY 4 cut(s) 179, 212, 854, 866
XceI RCATGY 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 38
ZrmI AGTACT 2 cut(s) 236, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.