MD11G1030900.v1.1

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008402
Physical Location & Seq
Reverse (-)
2684300 .. 2689266
4967 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1030900.v1.1.491

Sequence Viewer

Length: 2178 bp
ATGAACACTCACCGCCCGGTGAGGGCACTGTCACGTGCGATATCACGTGCCCTATCCCACTCCTCTTCCTCTTCGTCGTCCGTACAACAACAATCCTCCACCTTCCCTCTAAAAAACGTGACCAAATCCAACTTCGAGCCGGCCCTCGCCGACCTGCGCCGCCATGTCGCCGCCGCCGATTTCGTGGCCATCGATCTCGAGATGACCGGAATCACCAGCGCTCCGTGGCGTGACTCCCTGGAATTCGACCGCGCCGACGTCTGCTACCTTAAGGTCAAGGACTCGGCCGAGAAGTTCGCCGTGCTTCAGTTCGGAGTTTGCCCCTTCCGCTGGGACTCCTCCAAGCGCTCCTTCATTGCTCATCCGCACAATTTTTACATTTTTCCGCGTCAAGAGCTTCCGGTTGCAGGCCCCACTTATGAGTTTCTTTGCCAGACAACATCGATTGATTTCTTAGCTAAATACCAGTTCGATTTTAATGTCTGCATACATGAAGGAATATCTTATTTATCCAGAGGGCAGGAAAGTGAGGCACTGAGACGTTTGAGCTTGACATATGATGATGAGTTATTGACTAGATGTTCAAACTTGAAAGAACCTGTGCACATGCCAGTGGTCAAGATTGCTGACACTCTCTTTGCTGAACGGATGAAGAACACATTCACTGAATGGCGTGATGGTTTGTTGAACTTGAGAAGTAGTAATGGAGGATTCCAATTTCAGCAAAACTCAAATGATTCCCAACAGTTACAAACCATTTTCTTCAAGATGCGTCCAGCTCTTAGTTTGAAGGGATTCACTTCTCATCAGCTTAGGTTGATTCAAATGGTCATGAGAAAGCATTTCCAAGATCTTTCTTATGTTCAGGTAAATGGTGAAAAGTCGTGTCAACAGCAACTAGTTGTGCGGACAGATTCCAAGGATGACAGGGATTTGCTTCTGAAAGAGGTTAAGGATGAACATCGTAGGGAAGCAGAGGTGAAAGTTCAAGCTGCAGTTGGGTTTCGTCATGTTATTGACCTTCTTTCCTCAGAACAAAAGTTGATTGTTGGTCACAATTGCTTTCTTGATATTGCCCATATATACAACAAATTCTTGGGACCTCTACCTTCGACTGCTGAGGAATTTGTCTCTGCTGTTAACAAGTATTTTCCACACATCATTGACACCAAAGTACTGTTGAATACAGATAATGTGCTCCAACAACGGATGAAGAAGTCCAGAACATCACTTTCGTCAGCATTTGCTTTGTTATGCCCGCAAATTGCTCTTGGGAAGAAAAGCACTGATTCAGAAGTCCAATTGTGTGTCAAAGTTGAAGTTCAGGTGGATGATTTGAGGTCCTCCAACTGGAATTCTGGAGCCAAACATGAAGCAGGATACGATGCTTTTATGACAGGCTGTGTCTTTGCTCAGACATGCAGTCATCTCGGAATTGATTTTCAGGCTTGTTTGTCATCTGAAAAGTTGCCCCACAATGAGAAGCTCCAGAAGCAAATCAACCTTTTATACCTCAGTTGGAGTAATGGAGACATTATTGATTTAACCACCGGCAAGAAGAAAGCAATGTCTTCGGGGTATAATAATCACAAAAAGAGGTACGCGCAGATTATGTTTGAGAACATTGCTTTAATCTGGGGATTCCCATCAAAACTCAAGGCACGAGATATAAGGGAGTGCATCACAAAAGTCTTTGGCCCGACCTCTGTCACGTCTGTCTACCACTTGGATGAAACTGCAGTATTCATTCAGTTTAGCAAGGAAAAATTTGTCTCTGAATTTCTGGCGTTGAAGGAAACATTAGAGAGAAGTGATGGTCCAGTCTCGGTTTTGCATCCCCTCACGGGACTTTTGGAAGGTGGAAGCACCCGAGCTGCTAATTATGAAACTTACAAAGAAATCTGCAGTTCAGCTAGCGCAAAAGTCTTGTTTGCAGATCAGGCTGAGGCAGTTGGTATTAAATGGAAAACTAAATTGGTTGAATCCAAGGAAGCATCAGAGACCCTAAAACGAGAGATCTTCGATGAGAAAAGTGATATGAATCCTGCTTCGAAGTCTGAAAAACTGAGAAGTAACACAGATAATGCGCAAGATGATCCGTTGCGTGGGCGTCTCTCACATTACAAGATCATAGACACTTTAATTACTTCTGAAGGCGATCGAATCAGAACTAATTAG

Protein Analysis

726

Amino Acids

82.07

Weight (kDa)

8.46

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 40 - 470 6e-101 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
Acc16I TGCGCA 1 cut(s) 2088
Acc36I ACCTGC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 1719
AccII CGCG 3 cut(s) 252, 388, 1604
AclWI GGATC 1 cut(s) 2090
AcoI YGGCCR 2 cut(s) 186, 285
AcsI RAATTY 6 cut(s) 242, 1091, 1124, 1354, 1766, 1778
AcuI CTGAAG 2 cut(s) 290, 2172
AcvI CACGTG 2 cut(s) 35, 47
AcyI GRCGYC 2 cut(s) 258, 2110
AfaI GTAC 3 cut(s) 84, 1176, 1601
AfeI AGCGCT 2 cut(s) 220, 347
AfiI CCNNNNNNNGG 6 cut(s) 22, 330, 407, 1350, 1844, 2105
AflII CTTAAG 1 cut(s) 269
AhdI GACNNNNNGTC 1 cut(s) 1422
AhlI ACTAGT 1 cut(s) 898
AjiI CACGTC 1 cut(s) 1713
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 6 cut(s) 116, 148, 335, 367, 1958, 1990
AluBI AGCT 9 cut(s) 397, 458, 549, 779, 811, 992, 1486, 1874, 1913
AluI AGCT 9 cut(s) 397, 458, 549, 779, 811, 992, 1486, 1874, 1913
Alw21I GWGCWC 2 cut(s) 606, 1200
Alw26I GTCTC 7 cut(s) 532, 1135, 1524, 1777, 1828, 1994, 2117
Alw44I GTGCAC 1 cut(s) 602
AlwI GGATC 1 cut(s) 2090
Ama87I CYCGRG 2 cut(s) 197, 1869
Aor51HI AGCGCT 2 cut(s) 220, 347
AoxI GGCC 5 cut(s) 141, 186, 285, 409, 1696
ApaLI GTGCAC 1 cut(s) 602
ApeKI GCWGC 2 cut(s) 992, 1874
ApoI RAATTY 6 cut(s) 242, 1091, 1124, 1354, 1766, 1778
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
Asp700I GAANNNNTTC 2 cut(s) 659, 794
AspLEI GCGC 7 cut(s) 159, 221, 254, 348, 1606, 1919, 2089
AspS9I GGNCC 6 cut(s) 142, 410, 1100, 1341, 1697, 1817
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 4 cut(s) 31, 205, 887, 991
AsuII TTCGAA 1 cut(s) 2051
AsuNHI GCTAGC 1 cut(s) 1913
AvaI CYCGRG 2 cut(s) 197, 1869
AvaII GGWCC 3 cut(s) 1100, 1341, 1817
BaeGI GKGCMC 3 cut(s) 28, 52, 606
BalI TGGCCA 1 cut(s) 188
BauI CACGAG 1 cut(s) 1662
BbrPI CACGTG 2 cut(s) 35, 47
BbsI GAAGAC 1 cut(s) 1563
Bbv12I GWGCWC 2 cut(s) 606, 1200
BbvCI CCTCAGC 2 cut(s) 1119, 1944
BbvI GCAGC 2 cut(s) 979, 1861
BccI CCATC 4 cut(s) 197, 671, 1654, 1808
BceAI ACGGC 1 cut(s) 284
BcgI CGANNNNNNTGC 4 cut(s) 1411, 1445, 1554, 1588
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 1373
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 7 cut(s) 532, 1135, 1524, 1777, 1828, 1994, 2117
BcuI ACTAGT 1 cut(s) 898
BfaI CTAG 3 cut(s) 576, 899, 1914
BfmI CTRYAG 3 cut(s) 993, 1737, 1903
BfoI RGCGCY 2 cut(s) 222, 349
BfrI CTTAAG 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 162
BfuI GTATCC 1 cut(s) 1373
BglII AGATCT 2 cut(s) 850, 2016
BisI GCNGC 5 cut(s) 160, 171, 174, 993, 1875
BlsI GCNGC 5 cut(s) 161, 172, 175, 994, 1876
BmcAI AGTACT 1 cut(s) 1176
Bme1390I CCNGG 2 cut(s) 17, 239
Bme18I GGWCC 3 cut(s) 1100, 1341, 1817
BmeRI GACNNNNNGTC 1 cut(s) 1422
BmeT110I CYCGRG 2 cut(s) 197, 1869
BmgBI CACGTC 1 cut(s) 1713
BmgT120I GGNCC 6 cut(s) 142, 410, 1100, 1341, 1697, 1817
BmiI GGNNCC 3 cut(s) 412, 1101, 1363
BmrFI CCNGG 2 cut(s) 17, 239
BmsI GCATC 5 cut(s) 759, 1375, 1689, 1843, 2003
BmtI GCTAGC 1 cut(s) 1917
BoxI GACNNNNGTC 1 cut(s) 1706
BpiI GAAGAC 1 cut(s) 1563
BpmI CTGGAG 2 cut(s) 1380, 1472
Bpu10I CCTNAGC 3 cut(s) 812, 1119, 1944
Bpu14I TTCGAA 1 cut(s) 2051
BpuEI CTTGAG 2 cut(s) 712, 1640
BpuMI CCSGG 1 cut(s) 17
Bsa29I ATCGAT 2 cut(s) 192, 443
BsaAI YACGTR 2 cut(s) 35, 47
BsaBI GATNNNNATC 3 cut(s) 960, 1645, 2040
BsaHI GRCGYC 2 cut(s) 258, 2110
BsaI GGTCTC 1 cut(s) 1994
BsaJI CCNNGG 4 cut(s) 224, 237, 918, 1986
BsaWI WCCGGW 2 cut(s) 206, 400
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 6 cut(s) 22, 330, 407, 1350, 1844, 2105
Bse118I RCCGGY 2 cut(s) 139, 1550
Bse1I ACTGG 4 cut(s) 466, 611, 1355, 1820
Bse3DI GCAATG 3 cut(s) 354, 1572, 1623
Bse8I GATNNNNATC 3 cut(s) 960, 1645, 2040
BseBI CCWGG 1 cut(s) 239
BseCI ATCGAT 2 cut(s) 192, 443
BseDI CCNNGG 4 cut(s) 224, 237, 918, 1986
BseGI GGATG 8 cut(s) 361, 654, 928, 961, 1215, 1336, 1735, 1834
BseJI GATNNNNATC 3 cut(s) 960, 1645, 2040
BseLI CCNNNNNNNGG 6 cut(s) 22, 330, 407, 1350, 1844, 2105
BseMI GCAATG 3 cut(s) 354, 1572, 1623
BseMII CTCAG 7 cut(s) 527, 1044, 1110, 1427, 1528, 1935, 2057
BseNI ACTGG 4 cut(s) 466, 611, 1355, 1820
BseRI GAGGAG 2 cut(s) 52, 328
BseSI GKGCMC 3 cut(s) 28, 52, 606
BseX3I CGGCCG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 979, 1861
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 3 cut(s) 252, 388, 1604
Bsh1285I CGRYCG 3 cut(s) 250, 288, 2161
BshFI GGCC 5 cut(s) 143, 188, 287, 411, 1698
BshVI ATCGAT 2 cut(s) 192, 443
BsiEI CGRYCG 3 cut(s) 250, 288, 2161
BsiHKAI GWGCWC 2 cut(s) 606, 1200
BsiHKCI CYCGRG 2 cut(s) 197, 1869
BsiSI CCGG 5 cut(s) 17, 140, 207, 401, 1551
BslFI GGGAC 3 cut(s) 347, 1113, 1860
BslI CCNNNNNNNGG 6 cut(s) 22, 330, 407, 1350, 1844, 2105
BsmAI GTCTC 7 cut(s) 532, 1135, 1524, 1777, 1828, 1994, 2117
BsmBI CGTCTC 2 cut(s) 532, 2117
BsmFI GGGAC 3 cut(s) 347, 1113, 1860
BsnI GGCC 5 cut(s) 143, 188, 287, 411, 1698
Bso31I GGTCTC 1 cut(s) 1994
BsoBI CYCGRG 2 cut(s) 197, 1869
Bsp119I TTCGAA 1 cut(s) 2051
Bsp1286I GDGCHC 4 cut(s) 28, 52, 606, 1200
Bsp143I GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
BspANI GGCC 5 cut(s) 143, 188, 287, 411, 1698
BspCNI CTCAG 7 cut(s) 528, 1043, 1111, 1426, 1527, 1936, 2058
BspDI ATCGAT 2 cut(s) 192, 443
BspFNI CGCG 3 cut(s) 252, 388, 1604
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 3 cut(s) 412, 1101, 1363
BspMAI CTGCAG 3 cut(s) 997, 1741, 1907
BspMI ACCTGC 1 cut(s) 162
BspOI GCTAGC 1 cut(s) 1917
BspPI GGATC 1 cut(s) 2090
BspT104I TTCGAA 1 cut(s) 2051
BspTI CTTAAG 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 1994
BsrDI GCAATG 3 cut(s) 354, 1572, 1623
BsrFI RCCGGY 2 cut(s) 139, 1550
BsrI ACTGG 4 cut(s) 466, 611, 1355, 1820
BssAI RCCGGY 2 cut(s) 139, 1550
BssECI CCNNGG 4 cut(s) 224, 237, 918, 1986
BssMI GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
BssNI GRCGYC 2 cut(s) 258, 2110
BssSI CACGAG 1 cut(s) 1662
BssT1I CCWWGG 2 cut(s) 918, 1986
Bst2BI CACGAG 1 cut(s) 1662
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 3 cut(s) 30, 747, 1179
Bst6I CTCTTC 2 cut(s) 70, 76
BstACI GRCGYC 2 cut(s) 258, 2110
BstAFI CTTAAG 1 cut(s) 269
BstBAI YACGTR 2 cut(s) 35, 47
BstBI TTCGAA 1 cut(s) 2051
BstC8I GCNNGC 4 cut(s) 141, 409, 1259, 1915
BstDSI CCRYGG 1 cut(s) 224
BstF5I GGATG 8 cut(s) 361, 654, 928, 961, 1215, 1336, 1735, 1834
BstFNI CGCG 3 cut(s) 252, 388, 1604
BstH2I RGCGCY 2 cut(s) 222, 349
BstHHI GCGC 7 cut(s) 159, 221, 254, 348, 1606, 1919, 2089
BstKTI GATC 7 cut(s) 196, 853, 1939, 2019, 2098, 2130, 2161
BstMAI GTCTC 7 cut(s) 532, 1135, 1524, 1777, 1828, 1994, 2117
BstMBI GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
BstMCI CGRYCG 3 cut(s) 250, 288, 2161
BstMWI GCNNNNNNNGC 4 cut(s) 327, 394, 1492, 1940
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 2 cut(s) 610, 1422
BstPAI GACNNNNGTC 1 cut(s) 1706
BstSCI CCNGG 2 cut(s) 15, 237
BstSFI CTRYAG 3 cut(s) 993, 1737, 1903
BstSLI GKGCMC 3 cut(s) 28, 52, 606
BstUI CGCG 3 cut(s) 252, 388, 1604
BstV1I GCAGC 2 cut(s) 979, 1861
BstV2I GAAGAC 1 cut(s) 1563
BstX2I RGATCY 2 cut(s) 850, 2016
BstYI RGATCY 2 cut(s) 850, 2016
BstZI CGGCCG 1 cut(s) 285
Bsu15I ATCGAT 2 cut(s) 192, 443
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 5 cut(s) 143, 188, 287, 411, 1698
BsuTUI ATCGAT 2 cut(s) 192, 443
BtgI CCRYGG 1 cut(s) 224
BtrI CACGTC 1 cut(s) 1713
BtsCI GGATG 8 cut(s) 361, 654, 928, 961, 1215, 1336, 1735, 1834
BtsIMutI CAGTG 5 cut(s) 26, 533, 618, 663, 1284
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 4 cut(s) 141, 409, 1259, 1915
CciI TCATGA 1 cut(s) 831
CfoI GCGC 7 cut(s) 159, 221, 254, 348, 1606, 1919, 2089
Cfr10I RCCGGY 2 cut(s) 139, 1550
Cfr13I GGNCC 6 cut(s) 142, 410, 1100, 1341, 1697, 1817
ClaI ATCGAT 2 cut(s) 192, 443
CseI GACGC 3 cut(s) 377, 761, 2099
Csp6I GTAC 3 cut(s) 83, 1175, 1600
CviAII CATG 7 cut(s) 164, 491, 607, 832, 1010, 1370, 1419
CviQI GTAC 3 cut(s) 83, 1175, 1600
DpnI GATC 7 cut(s) 195, 852, 1938, 2018, 2097, 2129, 2160
DpnII GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
DriI GACNNNNNGTC 1 cut(s) 1422
EaeI YGGCCR 2 cut(s) 186, 285
EagI CGGCCG 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 70, 76
Eam1105I GACNNNNNGTC 1 cut(s) 1422
EarI CTCTTC 2 cut(s) 70, 76
EclXI CGGCCG 1 cut(s) 285
Eco130I CCWWGG 2 cut(s) 918, 1986
Eco31I GGTCTC 1 cut(s) 1994
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 3 cut(s) 1100, 1341, 1817
Eco47III AGCGCT 2 cut(s) 220, 347
Eco52I CGGCCG 1 cut(s) 285
Eco57I CTGAAG 2 cut(s) 290, 2172
Eco72I CACGTG 2 cut(s) 35, 47
Eco88I CYCGRG 2 cut(s) 197, 1869
EcoO109I RGGNCCY 3 cut(s) 410, 1100, 1341
EcoRI GAATTC 2 cut(s) 242, 1354
EcoRII CCWGG 1 cut(s) 237
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 2 cut(s) 918, 1986
ErhI CCWWGG 2 cut(s) 918, 1986
Esp3I CGTCTC 2 cut(s) 532, 2117
FaeI CATG 7 cut(s) 167, 494, 610, 835, 1013, 1373, 1422
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 3 cut(s) 347, 1113, 1860
FatI CATG 7 cut(s) 163, 490, 606, 831, 1009, 1369, 1418
FauI CCCGC 1 cut(s) 1266
FauNDI CATATG 1 cut(s) 556
FblI GTMKAC 1 cut(s) 1719
Fnu4HI GCNGC 5 cut(s) 160, 171, 174, 993, 1875
FokI GGATG 8 cut(s) 348, 661, 935, 968, 1222, 1343, 1742, 1821
Fsp4HI GCNGC 5 cut(s) 160, 171, 174, 993, 1875
FspBI CTAG 3 cut(s) 576, 899, 1914
FspI TGCGCA 1 cut(s) 2088
GlaI GCGC 7 cut(s) 158, 220, 253, 347, 1605, 1918, 2088
GluI GCNGC 5 cut(s) 160, 171, 174, 993, 1875
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 2 cut(s) 1380, 1472
HaeII RGCGCY 2 cut(s) 222, 349
HaeIII GGCC 5 cut(s) 143, 188, 287, 411, 1698
HapII CCGG 5 cut(s) 17, 140, 207, 401, 1551
HgaI GACGC 3 cut(s) 377, 761, 2099
HhaI GCGC 7 cut(s) 159, 221, 254, 348, 1606, 1919, 2089
Hin1I GRCGYC 2 cut(s) 258, 2110
Hin1II CATG 7 cut(s) 167, 494, 610, 835, 1013, 1373, 1422
Hin6I GCGC 7 cut(s) 157, 219, 252, 346, 1604, 1917, 2087
HinP1I GCGC 7 cut(s) 157, 219, 252, 346, 1604, 1917, 2087
HincII GTYRAC 2 cut(s) 890, 1141
HindII GTYRAC 2 cut(s) 890, 1141
HpaI GTTAAC 1 cut(s) 1141
HpaII CCGG 5 cut(s) 17, 140, 207, 401, 1551
HphI GGTGA 4 cut(s) 31, 205, 887, 991
Hpy166II GTNNAC 4 cut(s) 604, 890, 1141, 1720
Hpy8I GTNNAC 4 cut(s) 604, 890, 1141, 1720
Hpy99I CGWCG 2 cut(s) 79, 260
HpyCH4III ACNGT 3 cut(s) 30, 747, 1179
HpyCH4IV ACGT 6 cut(s) 34, 46, 117, 258, 541, 1712
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 394, 1492, 1940
HpySE526I ACGT 6 cut(s) 34, 46, 117, 258, 541, 1712
Hsp92I GRCGYC 2 cut(s) 258, 2110
Hsp92II CATG 7 cut(s) 167, 494, 610, 835, 1013, 1373, 1422
HspAI GCGC 7 cut(s) 157, 219, 252, 346, 1604, 1917, 2087
KroI GCCGGC 1 cut(s) 139
KroNI GCCGGC 1 cut(s) 141
KspAI GTTAAC 1 cut(s) 1141
Kzo9I GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
LmnI GCTCC 5 cut(s) 226, 353, 1203, 1361, 1491
Lsp1109I GCAGC 2 cut(s) 979, 1861
LweI GCATC 5 cut(s) 759, 1375, 1689, 1843, 2003
MaeI CTAG 3 cut(s) 576, 899, 1914
MaeII ACGT 6 cut(s) 34, 46, 117, 258, 541, 1712
MaeIII GTNAC 7 cut(s) 30, 118, 230, 747, 1052, 1708, 2072
MalI GATC 7 cut(s) 195, 852, 1938, 2018, 2097, 2129, 2160
MboI GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
MboII GAAGA 9 cut(s) 57, 63, 664, 754, 1225, 1288, 1563, 1570, 2011
MfeI CAATTG 2 cut(s) 1057, 1301
MflI RGATCY 2 cut(s) 850, 2016
MhlI GDGCHC 4 cut(s) 28, 52, 606, 1200
MlsI TGGCCA 1 cut(s) 188
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 3 cut(s) 227, 275, 329
MmeI TCCRAC 4 cut(s) 153, 1225, 1371, 1499
Mox20I TGGCCA 1 cut(s) 188
MroNI GCCGGC 1 cut(s) 139
MroXI GAANNNNTTC 2 cut(s) 659, 794
MscI TGGCCA 1 cut(s) 188
MseI TTAA 8 cut(s) 270, 477, 951, 1140, 1544, 1631, 1959, 2141
MslI CAYNNNNRTG 2 cut(s) 611, 1728
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 330
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 5 cut(s) 17, 140, 207, 401, 1551
MspR9I CCNGG 2 cut(s) 17, 239
MunI CAATTG 2 cut(s) 1057, 1301
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 3 cut(s) 252, 388, 1604
MwoI GCNNNNNNNGC 4 cut(s) 327, 394, 1492, 1940
NaeI GCCGGC 1 cut(s) 141
NciI CCSGG 1 cut(s) 17
NdeI CATATG 1 cut(s) 556
NdeII GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
NgoMIV GCCGGC 1 cut(s) 139
NheI GCTAGC 1 cut(s) 1913
NlaIII CATG 7 cut(s) 167, 494, 610, 835, 1013, 1373, 1422
NlaIV GGNNCC 3 cut(s) 412, 1101, 1363
NmeAIII GCCGAG 2 cut(s) 263, 313
NmuCI GTSAC 5 cut(s) 30, 118, 230, 1052, 1708
NsbI TGCGCA 1 cut(s) 2088
NspI RCATGY 2 cut(s) 610, 1422
NspV TTCGAA 1 cut(s) 2051
PaeR7I CTCGAG 1 cut(s) 197
PagI TCATGA 1 cut(s) 831
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 189, 252
PdiI GCCGGC 1 cut(s) 141
PdmI GAANNNNTTC 2 cut(s) 659, 794
PkrI GCNGC 5 cut(s) 161, 172, 175, 994, 1876
Ple19I CGATCG 1 cut(s) 2161
PleI GAGTC 3 cut(s) 227, 275, 329
PmaCI CACGTG 2 cut(s) 35, 47
PmlI CACGTG 2 cut(s) 35, 47
PpsI GAGTC 3 cut(s) 227, 275, 329
Ppu21I YACGTR 2 cut(s) 35, 47
PpuMI RGGWCCY 2 cut(s) 1100, 1341
PshAI GACNNNNGTC 1 cut(s) 1706
Psp5II RGGWCCY 2 cut(s) 1100, 1341
Psp6I CCWGG 1 cut(s) 237
PspCI CACGTG 2 cut(s) 35, 47
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 412, 1101, 1363
PspPI GGNCC 6 cut(s) 142, 410, 1100, 1341, 1697, 1817
PspPPI RGGWCCY 2 cut(s) 1100, 1341
PstI CTGCAG 3 cut(s) 997, 1741, 1907
PsuI RGATCY 2 cut(s) 850, 2016
PvuI CGATCG 1 cut(s) 2161
RsaI GTAC 3 cut(s) 84, 1176, 1601
RsaNI GTAC 3 cut(s) 83, 1175, 1600
RseI CAYNNNNRTG 2 cut(s) 611, 1728
SaqAI TTAA 8 cut(s) 270, 477, 951, 1140, 1544, 1631, 1959, 2141
SatI GCNGC 5 cut(s) 160, 171, 174, 993, 1875
Sau3AI GATC 7 cut(s) 193, 850, 1936, 2016, 2095, 2127, 2158
Sau96I GGNCC 6 cut(s) 142, 410, 1100, 1341, 1697, 1817
ScaI AGTACT 1 cut(s) 1176
SchI GAGTC 3 cut(s) 227, 275, 329
ScrFI CCNGG 2 cut(s) 17, 239
SduI GDGCHC 4 cut(s) 28, 52, 606, 1200
SfaNI GCATC 5 cut(s) 759, 1375, 1689, 1843, 2003
SfcI CTRYAG 3 cut(s) 993, 1737, 1903
Sfr274I CTCGAG 1 cut(s) 197
SfuI TTCGAA 1 cut(s) 2051
SinI GGWCC 3 cut(s) 1100, 1341, 1817
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 611, 1728
SmlI CTYRAG 4 cut(s) 197, 269, 691, 1655
SmoI CTYRAG 4 cut(s) 197, 269, 691, 1655
SpeI ACTAGT 1 cut(s) 898
SspMI CTAG 3 cut(s) 576, 899, 1914
StyD4I CCNGG 2 cut(s) 15, 237
StyI CCWWGG 2 cut(s) 918, 1986
TaaI ACNGT 3 cut(s) 30, 747, 1179
TaiI ACGT 6 cut(s) 37, 49, 120, 261, 544, 1715
TatI WGTACW 1 cut(s) 1174
TauI GCSGC 3 cut(s) 162, 173, 176
Tru1I TTAA 8 cut(s) 270, 477, 951, 1140, 1544, 1631, 1959, 2141
Tru9I TTAA 8 cut(s) 270, 477, 951, 1140, 1544, 1631, 1959, 2141
TscAI CASTG 5 cut(s) 33, 540, 618, 670, 1291
TseFI GTSAC 5 cut(s) 30, 118, 230, 1052, 1708
TseI GCWGC 2 cut(s) 992, 1874
Tsp45I GTSAC 5 cut(s) 30, 118, 230, 1052, 1708
TspGWI ACGGA 5 cut(s) 70, 213, 661, 1222, 2088
TspRI CASTG 5 cut(s) 33, 540, 618, 670, 1291
Vha464I CTTAAG 1 cut(s) 269
VneI GTGCAC 1 cut(s) 602
VpaK11BI GGWCC 3 cut(s) 1100, 1341, 1817
XapI RAATTY 6 cut(s) 242, 1091, 1124, 1354, 1766, 1778
XceI RCATGY 2 cut(s) 610, 1422
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 1719
XmnI GAANNNNTTC 2 cut(s) 659, 794
XspI CTAG 3 cut(s) 576, 899, 1914
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.