Rh5DG538900

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008402
Physical Location & Seq
Forward (+)
83071058 .. 83076371
5314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG538900.1

Sequence Viewer

Length: 2160 bp
ATGAACACTCACCGCCCGGTGAGGGCGCTGTCACGTGCCCTGTCACGTGCTCTATCTCAGTCCTCCTCCTCCTCTTCCTCCGCCGCACAACCCTCCTTCCCTCTGAAAAACGTGACCAAATCGAACTTCGAGCCGGTTCTCGCCGAGCTCCGCCGCCACGTCGCCGCCGCCGACTTCGTGGCCGTCGACCTCGAAATGAGCGGAGTCACCAGCGCGCCGTGGCGGGACTCGTTGGAGTTCGACCGCTCTGACGTCAGCTACCTCAAGGTCAAGGACTCGGCGGAGAAGTTCGCCGTGCTTCAGTTCGGCGTTTGCCCCTTTCGCTGGGACTCCTCCAAGAAATCCTTCATTGCTCATCCGCACAATTTCTATGTTTTTCCACGTCAAGAGCTTCCTGTCGAAAGCCCCACTTATGAGTTTCTCTGCCAGACTACATCAATTGACTTCTTAGCTAAATACCAGTTCGATTTCAATATGTGTATACATGAAGGAATATCTTATTTATCTAGAGGGCAGGAAAATGAAGCACTGAGACGTTTTCATTTGGCATATGAGGATGGATCTAAGTTGAAAGAAGTAAGGGATGCCCCTTTGGTTAAGATGGCTGACACTCTCTTCACTGAACGGATGAAGAATACATTTACTGAGTGGCGTGATGGGTTGTTCAGAAATAAAAATGGAGGGTTCCAATTTCAGGGAATCTCAAATGACCGCCAGCAACATTTAGAAACCATTTTTTTCAAGATGCGTCCTGCTCTTAGTCTAAATGGATTTACATCTCATCAGCTTAAATTGATTCAACTGGTCATTAGAAGGCATTTCCAAGATCTTTCTTATGTTCAGGTAAATGGGGAAAATTCATGTCAACAGCAACTAGTTGTGCATACAGATTCGAAGGATGACAGGGAGTCACTTATGAAAGAGGTGAAGGATGAGCATCTTAGACAAGCAGAGACCAAGGTTCAAGCTGCGGTGGGGTTTCGTCATGTTATTGACCTTCTTTCCTCAGAAAAAAAGCTGATTGTTGGTCACAATTGCTTTCTGGATATTGCACATATATACAGCAAATTTGTAGGTCCTCTTCCTTCAACTGCTGAAGAATTTGTATCTTCTGTTAACAAGTGTTTTCCGCACATCCTTGACACCAAAGTACTCTTGAATGCTAATATTGGGCTTCAACAACGGATGAAGAAATCCCAAACATCACTCTCGTCAGCATTTGCATCATTATGTCCACAAATTTCACTGGGGGCGAAAAGCACTGATCTGGCATTCCAATTGTGTGTCAAGGTTGAGGTTCAGGTTGATGATTTGAGGTCCTCCAACTGGAATTCTGGAGCCAAACATGAAGCTGGATATGATGCTTTTATGACAGGATGTGTCTTTGCTCAGGCCTGCAGTCATCTAGGTATTGATTTTGAAGCATGTTTGCCCTCTGAAAAGTTGTCCCACAATGAAAAGCTCCAGAAGCACATCAACCTTCTATTCCTTAGTTGGAGTAAAGGAGATGTTATTGATTTAACAACTGGAAATAAGAATGCGATGTCTGTAGGATCTAAGAACAACAAAAAACGTTACTTGCAGATTTCATATGAGAAAATTGCTTTAATATGGGGATTTTCATCCAAGCTCAAGGCACGAGAGATAAGAGAATGCATCTCCAAAGTCTTTGGCCTGACCTCAGTTGTATCTATCTATCACTTGGATGAAACTGCAGTGTTTGTTCAGTTTAACAAGGAGAAATTGGTCTCTGAGTTTCTCAGTTTGAAGGACACTTTAGAGAGAAAAGATGGTCCTGTCTCGGTTTTGCATCCTCTTGCAGGACTTTTGGAAGGTGGAAATACCCGAGCTGCTGATTATGAAACTTACAAAGAAATTTGTAGTTCACCCAACGCAAAAGTCTTGTTTGCAGATCAGGCAGAGGCATTTGGTATCCAATGGAAAACCAAGTTGGTCGAAGCTAAGGCATCATTGTGCCTAGAATGTGAGAGTTCAGGTGAAAAAAATGCTTTGAATCCTGCTTCAAAGTCCGATGGAAAATCTGAAAGAAAGAACATGGAAAATGTGCAGAGTGATTCATCTACTGCATGTGTCTCAAACTATAAGATTATAGATTCTTTGATTGCTTCTAGAGGCGATAAGGTCAGAACTAATTTGTGA

Protein Analysis

719

Amino Acids

80.73

Weight (kDa)

8.42

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 38 - 462 7.1e-103 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
AccBSI CCGCTC 2 cut(s) 201, 246
AccI GTMKAC 2 cut(s) 186, 481
AccII CGCG 1 cut(s) 215
AclI AACGTT 1 cut(s) 1573
AclWI GGATC 2 cut(s) 568, 1561
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 6 cut(s) 856, 1067, 1100, 1239, 1330, 1875
AcuI CTGAAG 2 cut(s) 284, 1116
AcvI CACGTG 2 cut(s) 35, 47
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 1 cut(s) 1152
AfiI CCNNNNNNNGG 5 cut(s) 22, 324, 694, 1326, 1820
AhdI GACNNNNNGTC 1 cut(s) 907
AhlI ACTAGT 1 cut(s) 874
AjiI CACGTC 2 cut(s) 160, 383
AjuI GAANNNNNNNTTGG 6 cut(s) 110, 142, 329, 361, 1934, 1966
Alw21I GWGCWC 2 cut(s) 52, 150
Alw26I GTCTC 5 cut(s) 526, 947, 1753, 1804, 2098
AlwI GGATC 2 cut(s) 568, 1561
Ama87I CYCGRG 1 cut(s) 1845
AoxI GGCC 3 cut(s) 180, 1392, 1672
ApeKI GCWGC 2 cut(s) 968, 1850
ApoI RAATTY 6 cut(s) 856, 1067, 1100, 1239, 1330, 1875
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 3 cut(s) 28, 215, 217
AspS9I GGNCC 3 cut(s) 1076, 1317, 1793
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 5 cut(s) 31, 199, 937, 1878, 2009
AsuII TTCGAA 1 cut(s) 893
AvaI CYCGRG 1 cut(s) 1845
AvaII GGWCC 3 cut(s) 1076, 1317, 1793
BaeGI GKGCMC 1 cut(s) 40
BanII GRGCYC 1 cut(s) 150
BarI GAAGNNNNNNTAC 2 cut(s) 1065, 1097
BauI CACGAG 1 cut(s) 1638
BbrPI CACGTG 2 cut(s) 35, 47
Bbv12I GWGCWC 2 cut(s) 52, 150
BbvI GCAGC 2 cut(s) 955, 1837
BccI CCATC 5 cut(s) 551, 595, 650, 1784, 2027
BceAI ACGGC 3 cut(s) 167, 202, 278
BciVI GTATCC 1 cut(s) 1943
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 5 cut(s) 526, 947, 1753, 1804, 2098
BcuI ACTAGT 1 cut(s) 874
BfaI CTAG 5 cut(s) 507, 875, 1406, 1979, 2130
BfmI CTRYAG 3 cut(s) 1396, 1548, 1713
BfoI RGCGCY 1 cut(s) 29
BfuI GTATCC 1 cut(s) 1943
BglII AGATCT 1 cut(s) 826
BisI GCNGC 6 cut(s) 84, 154, 165, 168, 969, 1851
BlsI GCNGC 6 cut(s) 85, 155, 166, 169, 970, 1852
BmcAI AGTACT 1 cut(s) 1152
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 3 cut(s) 1076, 1317, 1793
BmeRI GACNNNNNGTC 1 cut(s) 907
BmeT110I CYCGRG 1 cut(s) 1845
BmgBI CACGTC 2 cut(s) 160, 383
BmgT120I GGNCC 3 cut(s) 1076, 1317, 1793
BmiI GGNNCC 2 cut(s) 686, 1339
BmrFI CCNGG 1 cut(s) 17
BmrI ACTGGG 1 cut(s) 1256
BmsI GCATC 8 cut(s) 574, 735, 946, 1232, 1351, 1665, 1819, 1976
BmuI ACTGGG 1 cut(s) 1256
BpmI CTGGAG 2 cut(s) 1356, 1448
Bpu10I CCTNAGC 2 cut(s) 1389, 1962
Bpu14I TTCGAA 1 cut(s) 893
BpuEI CTTGAG 2 cut(s) 248, 1616
BpuMI CCSGG 1 cut(s) 17
BsaAI YACGTR 2 cut(s) 35, 47
BsaBI GATNNNNATC 3 cut(s) 775, 936, 1621
BsaHI GRCGYC 1 cut(s) 252
BsaI GGTCTC 2 cut(s) 947, 1753
BsaJI CCNNGG 2 cut(s) 218, 957
Bsc4I CCNNNNNNNGG 5 cut(s) 22, 324, 694, 1326, 1820
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 5 cut(s) 460, 807, 1251, 1331, 1531
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 3 cut(s) 775, 936, 1621
BseDI CCNNGG 2 cut(s) 218, 957
BseJI GATNNNNATC 3 cut(s) 775, 936, 1621
BseLI CCNNNNNNNGG 5 cut(s) 22, 324, 694, 1326, 1820
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 8 cut(s) 71, 521, 636, 1020, 1403, 1695, 1743, 1774
BseNI ACTGG 5 cut(s) 460, 807, 1251, 1331, 1531
BsePI GCGCGC 1 cut(s) 213
BseRI GAGGAG 4 cut(s) 55, 58, 61, 322
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 2 cut(s) 955, 1837
BseYI CCCAGC 1 cut(s) 324
BsgI GTGCAG 1 cut(s) 2087
Bsh1236I CGCG 1 cut(s) 215
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 3 cut(s) 182, 1394, 1674
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 52, 150
BsiHKCI CYCGRG 1 cut(s) 1845
BsiSI CCGG 2 cut(s) 17, 134
BslFI GGGAC 3 cut(s) 239, 341, 1432
BslI CCNNNNNNNGG 5 cut(s) 22, 324, 694, 1326, 1820
BsmAI GTCTC 5 cut(s) 526, 947, 1753, 1804, 2098
BsmBI CGTCTC 1 cut(s) 526
BsmFI GGGAC 3 cut(s) 239, 341, 1432
BsmI GAATGC 4 cut(s) 1165, 1271, 1543, 1658
BsnI GGCC 3 cut(s) 182, 1394, 1674
Bso31I GGTCTC 2 cut(s) 947, 1753
BsoBI CYCGRG 1 cut(s) 1845
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 3 cut(s) 40, 52, 150
Bsp143I GATC 5 cut(s) 560, 826, 1264, 1553, 1912
BspANI GGCC 3 cut(s) 182, 1394, 1674
BspCNI CTCAG 8 cut(s) 70, 522, 637, 1019, 1402, 1694, 1744, 1773
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 686, 1339
BspMAI CTGCAG 2 cut(s) 1400, 1717
BspPI GGATC 2 cut(s) 568, 1561
BspT104I TTCGAA 1 cut(s) 893
BspTNI GGTCTC 2 cut(s) 947, 1753
BsrBI CCGCTC 2 cut(s) 201, 246
BsrDI GCAATG 1 cut(s) 348
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 5 cut(s) 460, 807, 1251, 1331, 1531
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 218, 957
BssHII GCGCGC 1 cut(s) 213
BssMI GATC 5 cut(s) 560, 826, 1264, 1553, 1912
BssNAI GTATAC 1 cut(s) 482
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 1638
BssT1I CCWWGG 1 cut(s) 957
Bst1107I GTATAC 1 cut(s) 482
Bst2BI CACGAG 1 cut(s) 1638
Bst6I CTCTTC 3 cut(s) 79, 620, 1086
BstACI GRCGYC 1 cut(s) 252
BstBAI YACGTR 2 cut(s) 35, 47
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 3 cut(s) 215, 716, 1396
BstDSI CCRYGG 1 cut(s) 218
BstENI CCTNNNNNAGG 1 cut(s) 1818
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 3 cut(s) 28, 215, 217
BstKTI GATC 5 cut(s) 563, 829, 1267, 1556, 1915
BstMAI GTCTC 5 cut(s) 526, 947, 1753, 1804, 2098
BstMBI GATC 5 cut(s) 560, 826, 1264, 1553, 1912
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 321, 1468, 1916
BstNSI RCATGY 2 cut(s) 1428, 2091
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 3 cut(s) 1396, 1548, 1713
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 955, 1837
BstX2I RGATCY 3 cut(s) 560, 826, 1553
BstYI RGATCY 3 cut(s) 560, 826, 1553
BstZ17I GTATAC 1 cut(s) 482
BsuI GTATCC 1 cut(s) 1943
BsuRI GGCC 3 cut(s) 182, 1394, 1674
BtgI CCRYGG 1 cut(s) 218
BtgZI GCGATG 1 cut(s) 1556
BtrI CACGTC 2 cut(s) 160, 383
BtsI GCAGTG 1 cut(s) 1722
BtsIMutI CAGTG 5 cut(s) 527, 618, 1244, 1260, 1722
Cac8I GCNNGC 3 cut(s) 215, 716, 1396
CfoI GCGC 3 cut(s) 28, 215, 217
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 1076, 1317, 1793
CseI GACGC 1 cut(s) 737
Csp6I GTAC 1 cut(s) 1151
CviAII CATG 7 cut(s) 485, 861, 986, 1346, 1425, 2056, 2088
CviQI GTAC 1 cut(s) 1151
DpnI GATC 5 cut(s) 562, 828, 1266, 1555, 1914
DpnII GATC 5 cut(s) 560, 826, 1264, 1553, 1912
DriI GACNNNNNGTC 1 cut(s) 907
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 3 cut(s) 79, 620, 1086
Eam1105I GACNNNNNGTC 1 cut(s) 907
EarI CTCTTC 3 cut(s) 79, 620, 1086
EciI GGCGGA 3 cut(s) 70, 140, 296
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 957
Eco147I AGGCCT 1 cut(s) 1394
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 2 cut(s) 947, 1753
Eco47I GGWCC 3 cut(s) 1076, 1317, 1793
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 284, 1116
Eco72I CACGTG 2 cut(s) 35, 47
Eco88I CYCGRG 1 cut(s) 1845
EcoICRI GAGCTC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 1818
EcoO109I RGGNCCY 2 cut(s) 1076, 1317
EcoRI GAATTC 1 cut(s) 1330
EcoT14I CCWWGG 1 cut(s) 957
EcoT22I ATGCAT 1 cut(s) 1658
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 957
Esp3I CGTCTC 1 cut(s) 526
FaeI CATG 7 cut(s) 488, 864, 989, 1349, 1428, 2059, 2091
FalI AAGNNNNNCTT 4 cut(s) 329, 361, 394, 426
FaqI GGGAC 3 cut(s) 239, 341, 1432
FatI CATG 7 cut(s) 484, 860, 985, 1345, 1424, 2055, 2087
FauI CCCGC 1 cut(s) 216
FauNDI CATATG 2 cut(s) 550, 1591
FblI GTMKAC 2 cut(s) 186, 481
Fnu4HI GCNGC 6 cut(s) 84, 154, 165, 168, 969, 1851
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 6 cut(s) 84, 154, 165, 168, 969, 1851
FspBI CTAG 5 cut(s) 507, 875, 1406, 1979, 2130
GlaI GCGC 3 cut(s) 27, 214, 216
GluI GCNGC 6 cut(s) 84, 154, 165, 168, 969, 1851
GsaI CCCAGC 1 cut(s) 328
GsuI CTGGAG 2 cut(s) 1356, 1448
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 3 cut(s) 182, 1394, 1674
HapII CCGG 2 cut(s) 17, 134
HgaI GACGC 1 cut(s) 737
HhaI GCGC 3 cut(s) 28, 215, 217
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 7 cut(s) 488, 864, 989, 1349, 1428, 2059, 2091
Hin6I GCGC 3 cut(s) 26, 213, 215
HinP1I GCGC 3 cut(s) 26, 213, 215
HincII GTYRAC 3 cut(s) 187, 866, 1117
HindII GTYRAC 3 cut(s) 187, 866, 1117
HpaI GTTAAC 1 cut(s) 1117
HpaII CCGG 2 cut(s) 17, 134
HphI GGTGA 5 cut(s) 31, 199, 937, 1878, 2009
Hpy166II GTNNAC 6 cut(s) 187, 482, 866, 1117, 1235, 1886
Hpy188I TCNGA 9 cut(s) 105, 250, 668, 1009, 1438, 1753, 2032, 2044, 2147
Hpy188III TCNNGA 8 cut(s) 386, 507, 742, 1043, 1156, 1335, 1465, 2130
Hpy8I GTNNAC 6 cut(s) 187, 482, 866, 1117, 1235, 1886
Hpy99I CGWCG 2 cut(s) 164, 188
HpyCH4IV ACGT 8 cut(s) 34, 46, 111, 159, 252, 382, 535, 1573
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 1468, 1916
HpySE526I ACGT 8 cut(s) 34, 46, 111, 159, 252, 382, 535, 1573
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 7 cut(s) 488, 864, 989, 1349, 1428, 2059, 2091
HspAI GCGC 3 cut(s) 26, 213, 215
KspAI GTTAAC 1 cut(s) 1117
Kzo9I GATC 5 cut(s) 560, 826, 1264, 1553, 1912
LmnI GCTCC 3 cut(s) 153, 1337, 1467
Lsp1109I GCAGC 2 cut(s) 955, 1837
LweI GCATC 8 cut(s) 574, 735, 946, 1232, 1351, 1665, 1819, 1976
MaeI CTAG 5 cut(s) 507, 875, 1406, 1979, 2130
MaeII ACGT 8 cut(s) 34, 46, 111, 159, 252, 382, 535, 1573
MaeIII GTNAC 7 cut(s) 30, 42, 112, 205, 909, 1028, 1574
MalI GATC 5 cut(s) 562, 828, 1266, 1555, 1914
MbiI CCGCTC 2 cut(s) 201, 246
MboI GATC 5 cut(s) 560, 826, 1264, 1553, 1912
MboII GAAGA 7 cut(s) 66, 607, 643, 1073, 1101, 1109, 1201
MfeI CAATTG 3 cut(s) 438, 1033, 1277
MflI RGATCY 3 cut(s) 560, 826, 1553
MhlI GDGCHC 3 cut(s) 40, 52, 150
MlyI GAGTC 5 cut(s) 213, 221, 269, 323, 917
MmeI TCCRAC 3 cut(s) 213, 1347, 1475
Mph1103I ATGCAT 1 cut(s) 1658
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 6 cut(s) 597, 789, 1116, 1520, 1607, 1731
MslI CAYNNNNRTG 3 cut(s) 1228, 1704, 1972
MspI CCGG 2 cut(s) 17, 134
MspR9I CCNGG 1 cut(s) 17
MunI CAATTG 3 cut(s) 438, 1033, 1277
Mva1269I GAATGC 4 cut(s) 1165, 1271, 1543, 1658
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 3 cut(s) 321, 1468, 1916
NciI CCSGG 1 cut(s) 17
NdeI CATATG 2 cut(s) 550, 1591
NdeII GATC 5 cut(s) 560, 826, 1264, 1553, 1912
NlaIII CATG 7 cut(s) 488, 864, 989, 1349, 1428, 2059, 2091
NlaIV GGNNCC 2 cut(s) 686, 1339
NmeAIII GCCGAG 2 cut(s) 169, 257
NmuCI GTSAC 6 cut(s) 30, 42, 112, 205, 909, 1028
NsiI ATGCAT 1 cut(s) 1658
NspI RCATGY 2 cut(s) 1428, 2091
NspV TTCGAA 1 cut(s) 893
PauI GCGCGC 1 cut(s) 213
PceI AGGCCT 1 cut(s) 1394
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 183
PctI GAATGC 4 cut(s) 1165, 1271, 1543, 1658
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 6 cut(s) 699, 796, 890, 2014, 2075, 2114
PkrI GCNGC 6 cut(s) 85, 155, 166, 169, 970, 1852
PleI GAGTC 5 cut(s) 212, 221, 269, 323, 916
PmaCI CACGTG 2 cut(s) 35, 47
PmlI CACGTG 2 cut(s) 35, 47
PpsI GAGTC 5 cut(s) 212, 221, 269, 323, 916
Ppu21I YACGTR 2 cut(s) 35, 47
PpuMI RGGWCCY 2 cut(s) 1076, 1317
Psp124BI GAGCTC 1 cut(s) 150
Psp1406I AACGTT 1 cut(s) 1573
Psp5II RGGWCCY 2 cut(s) 1076, 1317
PspCI CACGTG 2 cut(s) 35, 47
PspFI CCCAGC 1 cut(s) 324
PspN4I GGNNCC 2 cut(s) 686, 1339
PspPI GGNCC 3 cut(s) 1076, 1317, 1793
PspPPI RGGWCCY 2 cut(s) 1076, 1317
PstI CTGCAG 2 cut(s) 1400, 1717
PsuI RGATCY 3 cut(s) 560, 826, 1553
PteI GCGCGC 1 cut(s) 213
RsaI GTAC 1 cut(s) 1152
RsaNI GTAC 1 cut(s) 1151
RseI CAYNNNNRTG 3 cut(s) 1228, 1704, 1972
SacI GAGCTC 1 cut(s) 150
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 6 cut(s) 597, 789, 1116, 1520, 1607, 1731
SatI GCNGC 6 cut(s) 84, 154, 165, 168, 969, 1851
Sau3AI GATC 5 cut(s) 560, 826, 1264, 1553, 1912
Sau96I GGNCC 3 cut(s) 1076, 1317, 1793
ScaI AGTACT 1 cut(s) 1152
SchI GAGTC 5 cut(s) 213, 221, 269, 323, 917
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 3 cut(s) 40, 52, 150
SfaNI GCATC 8 cut(s) 574, 735, 946, 1232, 1351, 1665, 1819, 1976
SfcI CTRYAG 3 cut(s) 1396, 1548, 1713
SfuI TTCGAA 1 cut(s) 893
SinI GGWCC 3 cut(s) 1076, 1317, 1793
SmiMI CAYNNNNRTG 3 cut(s) 1228, 1704, 1972
SmlI CTYRAG 2 cut(s) 263, 1631
SmoI CTYRAG 2 cut(s) 263, 1631
SpeI ACTAGT 1 cut(s) 874
SseBI AGGCCT 1 cut(s) 1394
SspI AATATT 1 cut(s) 1168
SspMI CTAG 5 cut(s) 507, 875, 1406, 1979, 2130
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 1394
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 1 cut(s) 957
TaiI ACGT 8 cut(s) 37, 49, 114, 162, 255, 385, 538, 1576
TaqI TCGA 9 cut(s) 122, 129, 186, 192, 240, 399, 465, 893, 1956
TatI WGTACW 1 cut(s) 1150
TauI GCSGC 4 cut(s) 86, 156, 167, 170
TfiI GAWTC 6 cut(s) 699, 796, 890, 2014, 2075, 2114
Tru1I TTAA 6 cut(s) 597, 789, 1116, 1520, 1607, 1731
Tru9I TTAA 6 cut(s) 597, 789, 1116, 1520, 1607, 1731
TscAI CASTG 5 cut(s) 534, 625, 1251, 1267, 1722
TseFI GTSAC 6 cut(s) 30, 42, 112, 205, 909, 1028
TseI GCWGC 2 cut(s) 968, 1850
Tsp45I GTSAC 6 cut(s) 30, 42, 112, 205, 909, 1028
TspGWI ACGGA 2 cut(s) 640, 1198
TspRI CASTG 5 cut(s) 534, 625, 1251, 1267, 1722
VpaK11BI GGWCC 3 cut(s) 1076, 1317, 1793
XagI CCTNNNNNAGG 1 cut(s) 1818
XapI RAATTY 6 cut(s) 856, 1067, 1100, 1239, 1330, 1875
XbaI TCTAGA 2 cut(s) 506, 2129
XceI RCATGY 2 cut(s) 1428, 2091
XmiI GTMKAC 2 cut(s) 186, 481
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 5 cut(s) 507, 875, 1406, 1979, 2130
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 1 cut(s) 1152
Zsp2I ATGCAT 1 cut(s) 1658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.