Rw5G047140

poly(A)-specific ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008402
Physical Location & Seq
Reverse (-)
81633225 .. 81638272
5048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047140.1

Sequence Viewer

Length: 2163 bp
ATGAACACTCACACTCACCGCCCGGTGAGGGCGCTGTCACGTGCCCTGTCACGTGCCCTATCCCAGTCTACCTCCTCTTCCTCCGCCGCCGCACAACCCTCCTTCCCTCTGAAAAACGTGACCAAATCGAACTTCGAGCCGGTTCTCGCCGAGCTCCGCCGCCACGTCGCCGCCGCCGACTTCGTGGCCGTCGACCTCGAAATGAGCGGAGTCACCAGCGCGCCGTGGCGGGACTCCTTGGAGTTCGACCGCTCTGACGTCAGCTACCTCAAGGTCAAGGACTCGGCGGAGAAGTTCGCCGTGCTTCAGTTCGGCGTTTGCCCCTTTCGCTGGGACTCCTCCAAGAAATCCTTCATTGCTCATCCGCACAATTTCTATGTTTTTCCACGTCAAGAGCTTCCTGTCGAAAGCCCCACTTATGAGTTTCTCTGCCAGACTACATCAATTGACTTCTTAGCTAAATACCAGTTCGATTTCAATATGTGTATACATGAAGGAATATCTTATTTATCTAGAGGGCAGGAAAATGAAGCACTGAGATGTTTTCATTTGGCATATGAGGATGGATCTAAGTTGAAAGAAGTAAGGGATGCCCCTTTGGTTAAGATGGCTGACACTCTCTTCACTGAACGGATGAAGAATACATTTACCGAGTGGCGTGATGGGTTGTTGAGAAATAAAAATGGAGGGTTCCAATTTCAGGGAATCTCAAATGACCGCCAGCAACATTTAGAAACCATTTTTTTCAAGATGCGTCCTGCTCTTAGTCTAAATGGATTTACATCTCATCAGCTTAAATTGATTCAACTGGTCATTAGAAGGCATTTCCAAGATCTTTCTTATGTTCAGGTAAATGGGGAAAATTCATGTCAACAGCAACTAGTTGTGCATACAGATTCGAAGGATGACAGGGAGTCACTTATGAAGGATGTGAAGGATGAACATCTTAGACAAGCAGAGACCAAGGTTCAAGCTGCGGTGGGGTTTCGTCATGTTATTGACCTTCTTTCCTCAGAAAAAAAGCTGATTGTTGGTCACAATTGCTTTCTGGATATTGCACATATATACAGCAAATTTGTAGGTCCTCTTCCTTCAACTGCTGAAGAATTTGTATCTTCTGTTAACAAGTGTTTTCCGCACATCCTTGACACCAAAGTACTCTTGAATGCTAATATTGGGCTTCAACAACGGATGAAGAAATCCCAAACATCGCTCTCGTCAGCATTTGCATCATTATGTCCACAAATTTCACTGGGGGCGAAAAGCACTGATCTGGCATTCCAATTGTGTGTCAAAGTTGAGGTTCAGGTTGATGATTTGAGGTCCTCCAACTGGAATTCTGGAGCCAAACATGAAGCTGGATATGATGCTTTTATGACAGGATGTGTCTTTGCCCAGGCCTGCAGTCATCTAGGTATTGATTTTGGAGCATGTTTGCCCTCTGAAAAGTTGGCCCACAATGAAAAGCTCCAGAAGCACATCAACCTTCTATTCCTTAGTTGGAGTAAAGGAGATGTTATTGATTTAACAACTGGAAATAAGAATGCGATGTCTGTGGGATCTAAGAACAACAAAAAACGTTACTTGCAGATTTCATATGAGAAAATTGCTTTAATATGGGGATTTTCATCCAAGCTCAAGGCACGAGAGATAAGAGAATGCATCTCCAAAGTCTTTGGCCTGACCTCAGTTGTATCTATCTATCACTTGGATGAAACTGCAGTGTTTGTTCAGTTTAACAAGGAGAAATTGGTCTCTGAGTTTCTCAGTTTGAAGGACACTTTAGAGAGAAAAGATGGTCCTGTCTCGGTTTTGCATCCTCTTGCAGGACTTTTGGAAGGTGGAAATACCCGAGCTGCTGATTATGAAACTTACAAAGAAATTTGTAGTTCACCCAATGCAAAAGTCTTGTTTGCAGATCAGGCAGAGGCATTTGGTATCCAATGGAAAACCAAGTTGGTCGAAGCTAAGGCATTGTGCCTAGAATGTGAGAGTTCAGGTGAAAAAAATGCTTTGAATCCTGCTTCAAAATCCAATGAAAAATCTGAAAGAAAGAACATGGAAAATGTGCAGAGTGATTCATCTACTGCATGTGTCTCAAACTATAAGATTATAGATTCTTTGATTGCCTCTAGAGCCGATATGGTCAGAACTAATTTGTGA

Protein Analysis

720

Amino Acids

80.78

Weight (kDa)

8.3

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 40 - 464 4.2e-102 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccBSI CCGCTC 2 cut(s) 207, 252
AccI GTMKAC 3 cut(s) 68, 192, 487
AccII CGCG 1 cut(s) 221
AclI AACGTT 1 cut(s) 1579
AclWI GGATC 2 cut(s) 574, 1567
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 6 cut(s) 862, 1073, 1106, 1245, 1336, 1881
AcuI CTGAAG 2 cut(s) 290, 1122
AcvI CACGTG 2 cut(s) 41, 53
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 1 cut(s) 1158
AfiI CCNNNNNNNGG 5 cut(s) 28, 330, 700, 1332, 1826
AhdI GACNNNNNGTC 1 cut(s) 913
AhlI ACTAGT 1 cut(s) 880
AjiI CACGTC 2 cut(s) 166, 389
AjnI CCWGG 1 cut(s) 1395
AjuI GAANNNNNNNTTGG 6 cut(s) 116, 148, 335, 367, 1940, 1972
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 4 cut(s) 953, 1759, 1810, 2101
AlwI GGATC 2 cut(s) 574, 1567
Ama87I CYCGRG 1 cut(s) 1851
AoxI GGCC 4 cut(s) 186, 1398, 1452, 1678
ApeKI GCWGC 2 cut(s) 974, 1856
ApoI RAATTY 6 cut(s) 862, 1073, 1106, 1245, 1336, 1881
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 3 cut(s) 34, 221, 223
AspS9I GGNCC 4 cut(s) 1082, 1323, 1453, 1799
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 5 cut(s) 8, 37, 205, 1884, 2012
AsuII TTCGAA 1 cut(s) 899
AvaI CYCGRG 1 cut(s) 1851
AvaII GGWCC 3 cut(s) 1082, 1323, 1799
BaeGI GKGCMC 2 cut(s) 46, 58
BanII GRGCYC 1 cut(s) 156
BarI GAAGNNNNNNTAC 2 cut(s) 1071, 1103
BauI CACGAG 1 cut(s) 1644
BbrPI CACGTG 2 cut(s) 41, 53
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 2 cut(s) 961, 1843
BccI CCATC 4 cut(s) 557, 601, 656, 1790
BceAI ACGGC 3 cut(s) 173, 208, 284
BciT130I CCWGG 1 cut(s) 1397
BciVI GTATCC 1 cut(s) 1949
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 4 cut(s) 953, 1759, 1810, 2101
BcuI ACTAGT 1 cut(s) 880
BfaI CTAG 5 cut(s) 513, 881, 1412, 1982, 2133
BfmI CTRYAG 2 cut(s) 1402, 1719
BfoI RGCGCY 1 cut(s) 35
BfuI GTATCC 1 cut(s) 1949
BglII AGATCT 1 cut(s) 832
BisI GCNGC 7 cut(s) 87, 90, 160, 171, 174, 975, 1857
BlsI GCNGC 7 cut(s) 88, 91, 161, 172, 175, 976, 1858
BmcAI AGTACT 1 cut(s) 1158
Bme1390I CCNGG 2 cut(s) 23, 1397
Bme18I GGWCC 3 cut(s) 1082, 1323, 1799
BmeRI GACNNNNNGTC 1 cut(s) 913
BmeT110I CYCGRG 1 cut(s) 1851
BmgBI CACGTC 2 cut(s) 166, 389
BmgT120I GGNCC 4 cut(s) 1082, 1323, 1453, 1799
BmiI GGNNCC 2 cut(s) 692, 1345
BmrFI CCNGG 2 cut(s) 23, 1397
BmrI ACTGGG 2 cut(s) 58, 1262
BmsI GCATC 6 cut(s) 580, 741, 1238, 1357, 1671, 1825
BmuI ACTGGG 2 cut(s) 58, 1262
BpmI CTGGAG 2 cut(s) 1362, 1454
Bpu10I CCTNAGC 1 cut(s) 1968
Bpu14I TTCGAA 1 cut(s) 899
BpuEI CTTGAG 2 cut(s) 254, 1622
BpuMI CCSGG 1 cut(s) 23
BsaAI YACGTR 2 cut(s) 41, 53
BsaBI GATNNNNATC 3 cut(s) 781, 942, 1627
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 2 cut(s) 953, 1759
BsaJI CCNNGG 4 cut(s) 224, 237, 963, 1395
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 330, 700, 1332, 1826
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 6 cut(s) 64, 466, 813, 1257, 1337, 1537
Bse3DI GCAATG 1 cut(s) 354
Bse8I GATNNNNATC 3 cut(s) 781, 942, 1627
BseBI CCWGG 1 cut(s) 1397
BseDI CCNNGG 4 cut(s) 224, 237, 963, 1395
BseJI GATNNNNATC 3 cut(s) 781, 942, 1627
BseLI CCNNNNNNNGG 5 cut(s) 28, 330, 700, 1332, 1826
BseMI GCAATG 1 cut(s) 354
BseMII CTCAG 5 cut(s) 527, 1026, 1701, 1749, 1780
BseNI ACTGG 6 cut(s) 64, 466, 813, 1257, 1337, 1537
BsePI GCGCGC 1 cut(s) 219
BseRI GAGGAG 2 cut(s) 64, 328
BseSI GKGCMC 2 cut(s) 46, 58
BseXI GCAGC 2 cut(s) 961, 1843
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 1 cut(s) 2090
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 4 cut(s) 188, 1400, 1454, 1680
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 1851
BsiSI CCGG 2 cut(s) 23, 140
BslFI GGGAC 2 cut(s) 245, 347
BslI CCNNNNNNNGG 5 cut(s) 28, 330, 700, 1332, 1826
BsmAI GTCTC 4 cut(s) 953, 1759, 1810, 2101
BsmFI GGGAC 2 cut(s) 245, 347
BsmI GAATGC 4 cut(s) 1171, 1277, 1549, 1664
BsnI GGCC 4 cut(s) 188, 1400, 1454, 1680
Bso31I GGTCTC 2 cut(s) 953, 1759
BsoBI CYCGRG 1 cut(s) 1851
Bsp119I TTCGAA 1 cut(s) 899
Bsp1286I GDGCHC 3 cut(s) 46, 58, 156
Bsp143I GATC 5 cut(s) 566, 832, 1270, 1559, 1918
BspANI GGCC 4 cut(s) 188, 1400, 1454, 1680
BspCNI CTCAG 5 cut(s) 528, 1025, 1700, 1750, 1779
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 692, 1345
BspMAI CTGCAG 2 cut(s) 1406, 1723
BspPI GGATC 2 cut(s) 574, 1567
BspT104I TTCGAA 1 cut(s) 899
BspTNI GGTCTC 2 cut(s) 953, 1759
BsrBI CCGCTC 2 cut(s) 207, 252
BsrDI GCAATG 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 139
BsrI ACTGG 6 cut(s) 64, 466, 813, 1257, 1337, 1537
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 4 cut(s) 224, 237, 963, 1395
BssHII GCGCGC 1 cut(s) 219
BssMI GATC 5 cut(s) 566, 832, 1270, 1559, 1918
BssNAI GTATAC 1 cut(s) 488
BssNI GRCGYC 1 cut(s) 258
BssSI CACGAG 1 cut(s) 1644
BssT1I CCWWGG 2 cut(s) 237, 963
Bst1107I GTATAC 1 cut(s) 488
Bst2BI CACGAG 1 cut(s) 1644
Bst2UI CCWGG 1 cut(s) 1397
Bst6I CTCTTC 3 cut(s) 82, 626, 1092
BstACI GRCGYC 1 cut(s) 258
BstBAI YACGTR 2 cut(s) 41, 53
BstBI TTCGAA 1 cut(s) 899
BstC8I GCNNGC 3 cut(s) 221, 722, 1402
BstDSI CCRYGG 1 cut(s) 224
BstENI CCTNNNNNAGG 1 cut(s) 1824
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 3 cut(s) 34, 221, 223
BstKTI GATC 5 cut(s) 569, 835, 1273, 1562, 1921
BstMAI GTCTC 4 cut(s) 953, 1759, 1810, 2101
BstMBI GATC 5 cut(s) 566, 832, 1270, 1559, 1918
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 4 cut(s) 327, 1474, 1922, 2135
BstNI CCWGG 1 cut(s) 1397
BstNSI RCATGY 2 cut(s) 1434, 2094
BstSCI CCNGG 2 cut(s) 21, 1395
BstSFI CTRYAG 2 cut(s) 1402, 1719
BstSLI GKGCMC 2 cut(s) 46, 58
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 961, 1843
BstX2I RGATCY 3 cut(s) 566, 832, 1559
BstYI RGATCY 3 cut(s) 566, 832, 1559
BstZ17I GTATAC 1 cut(s) 488
BsuI GTATCC 1 cut(s) 1949
BsuRI GGCC 4 cut(s) 188, 1400, 1454, 1680
BtgI CCRYGG 1 cut(s) 224
BtgZI GCGATG 2 cut(s) 1194, 1562
BtrI CACGTC 2 cut(s) 166, 389
BtsI GCAGTG 1 cut(s) 1728
BtsIMutI CAGTG 5 cut(s) 533, 624, 1250, 1266, 1728
Cac8I GCNNGC 3 cut(s) 221, 722, 1402
CfoI GCGC 3 cut(s) 34, 221, 223
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 4 cut(s) 1082, 1323, 1453, 1799
CseI GACGC 1 cut(s) 743
Csp6I GTAC 1 cut(s) 1157
CviAII CATG 7 cut(s) 491, 867, 992, 1352, 1431, 2059, 2091
CviQI GTAC 1 cut(s) 1157
DpnI GATC 5 cut(s) 568, 834, 1272, 1561, 1920
DpnII GATC 5 cut(s) 566, 832, 1270, 1559, 1918
DriI GACNNNNNGTC 1 cut(s) 913
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 3 cut(s) 82, 626, 1092
Eam1105I GACNNNNNGTC 1 cut(s) 913
EarI CTCTTC 3 cut(s) 82, 626, 1092
EciI GGCGGA 3 cut(s) 73, 146, 302
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 237, 963
Eco147I AGGCCT 1 cut(s) 1400
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 2 cut(s) 953, 1759
Eco47I GGWCC 3 cut(s) 1082, 1323, 1799
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 2 cut(s) 290, 1122
Eco72I CACGTG 2 cut(s) 41, 53
Eco88I CYCGRG 1 cut(s) 1851
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 1824
EcoO109I RGGNCCY 2 cut(s) 1082, 1323
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 1 cut(s) 1395
EcoT14I CCWWGG 2 cut(s) 237, 963
EcoT22I ATGCAT 1 cut(s) 1664
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 2 cut(s) 237, 963
FaeI CATG 7 cut(s) 494, 870, 995, 1355, 1434, 2062, 2094
FalI AAGNNNNNCTT 4 cut(s) 335, 367, 400, 432
FaqI GGGAC 2 cut(s) 245, 347
FatI CATG 7 cut(s) 490, 866, 991, 1351, 1430, 2058, 2090
FauI CCCGC 1 cut(s) 222
FauNDI CATATG 2 cut(s) 556, 1597
FblI GTMKAC 3 cut(s) 68, 192, 487
Fnu4HI GCNGC 7 cut(s) 87, 90, 160, 171, 174, 975, 1857
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 7 cut(s) 87, 90, 160, 171, 174, 975, 1857
FspBI CTAG 5 cut(s) 513, 881, 1412, 1982, 2133
GlaI GCGC 3 cut(s) 33, 220, 222
GluI GCNGC 7 cut(s) 87, 90, 160, 171, 174, 975, 1857
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 2 cut(s) 1362, 1454
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 4 cut(s) 188, 1400, 1454, 1680
HapII CCGG 2 cut(s) 23, 140
HgaI GACGC 1 cut(s) 743
HhaI GCGC 3 cut(s) 34, 221, 223
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 7 cut(s) 494, 870, 995, 1355, 1434, 2062, 2094
Hin6I GCGC 3 cut(s) 32, 219, 221
HinP1I GCGC 3 cut(s) 32, 219, 221
HincII GTYRAC 3 cut(s) 193, 872, 1123
HindII GTYRAC 3 cut(s) 193, 872, 1123
HpaI GTTAAC 1 cut(s) 1123
HpaII CCGG 2 cut(s) 23, 140
HphI GGTGA 5 cut(s) 8, 37, 205, 1884, 2012
Hpy166II GTNNAC 7 cut(s) 69, 193, 488, 872, 1123, 1241, 1892
Hpy188I TCNGA 7 cut(s) 111, 256, 1015, 1444, 1759, 2047, 2150
Hpy188III TCNNGA 8 cut(s) 392, 513, 748, 1049, 1162, 1341, 1471, 2133
Hpy8I GTNNAC 7 cut(s) 69, 193, 488, 872, 1123, 1241, 1892
Hpy99I CGWCG 2 cut(s) 170, 194
HpyCH4IV ACGT 7 cut(s) 40, 52, 117, 165, 258, 388, 1579
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 1474, 1922, 2135
HpySE526I ACGT 7 cut(s) 40, 52, 117, 165, 258, 388, 1579
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 7 cut(s) 494, 870, 995, 1355, 1434, 2062, 2094
HspAI GCGC 3 cut(s) 32, 219, 221
KspAI GTTAAC 1 cut(s) 1123
Kzo9I GATC 5 cut(s) 566, 832, 1270, 1559, 1918
LmnI GCTCC 4 cut(s) 159, 1343, 1427, 1473
Lsp1109I GCAGC 2 cut(s) 961, 1843
LweI GCATC 6 cut(s) 580, 741, 1238, 1357, 1671, 1825
MaeI CTAG 5 cut(s) 513, 881, 1412, 1982, 2133
MaeII ACGT 7 cut(s) 40, 52, 117, 165, 258, 388, 1579
MaeIII GTNAC 7 cut(s) 36, 48, 118, 211, 915, 1034, 1580
MalI GATC 5 cut(s) 568, 834, 1272, 1561, 1920
MbiI CCGCTC 2 cut(s) 207, 252
MboI GATC 5 cut(s) 566, 832, 1270, 1559, 1918
MboII GAAGA 7 cut(s) 69, 613, 649, 1079, 1107, 1115, 1207
MfeI CAATTG 3 cut(s) 444, 1039, 1283
MflI RGATCY 3 cut(s) 566, 832, 1559
MhlI GDGCHC 3 cut(s) 46, 58, 156
MlyI GAGTC 5 cut(s) 219, 227, 275, 329, 923
MmeI TCCRAC 2 cut(s) 1353, 1481
Mph1103I ATGCAT 1 cut(s) 1664
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 6 cut(s) 603, 795, 1122, 1526, 1613, 1737
MslI CAYNNNNRTG 3 cut(s) 538, 1234, 1710
MspI CCGG 2 cut(s) 23, 140
MspR9I CCNGG 2 cut(s) 23, 1397
MunI CAATTG 3 cut(s) 444, 1039, 1283
Mva1269I GAATGC 4 cut(s) 1171, 1277, 1549, 1664
MvaI CCWGG 1 cut(s) 1397
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 327, 1474, 1922, 2135
NciI CCSGG 1 cut(s) 23
NdeI CATATG 2 cut(s) 556, 1597
NdeII GATC 5 cut(s) 566, 832, 1270, 1559, 1918
NlaIII CATG 7 cut(s) 494, 870, 995, 1355, 1434, 2062, 2094
NlaIV GGNNCC 2 cut(s) 692, 1345
NmeAIII GCCGAG 2 cut(s) 175, 263
NmuCI GTSAC 6 cut(s) 36, 48, 118, 211, 915, 1034
NsiI ATGCAT 1 cut(s) 1664
NspI RCATGY 2 cut(s) 1434, 2094
NspV TTCGAA 1 cut(s) 899
PauI GCGCGC 1 cut(s) 219
PceI AGGCCT 1 cut(s) 1400
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 189
PctI GAATGC 4 cut(s) 1171, 1277, 1549, 1664
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 6 cut(s) 705, 802, 896, 2017, 2078, 2117
PkrI GCNGC 7 cut(s) 88, 91, 161, 172, 175, 976, 1858
PleI GAGTC 5 cut(s) 218, 227, 275, 329, 922
PmaCI CACGTG 2 cut(s) 41, 53
PmlI CACGTG 2 cut(s) 41, 53
PpsI GAGTC 5 cut(s) 218, 227, 275, 329, 922
Ppu21I YACGTR 2 cut(s) 41, 53
PpuMI RGGWCCY 2 cut(s) 1082, 1323
Psp124BI GAGCTC 1 cut(s) 156
Psp1406I AACGTT 1 cut(s) 1579
Psp5II RGGWCCY 2 cut(s) 1082, 1323
Psp6I CCWGG 1 cut(s) 1395
PspCI CACGTG 2 cut(s) 41, 53
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 1 cut(s) 1395
PspN4I GGNNCC 2 cut(s) 692, 1345
PspPI GGNCC 4 cut(s) 1082, 1323, 1453, 1799
PspPPI RGGWCCY 2 cut(s) 1082, 1323
PstI CTGCAG 2 cut(s) 1406, 1723
PsuI RGATCY 3 cut(s) 566, 832, 1559
PteI GCGCGC 1 cut(s) 219
RsaI GTAC 1 cut(s) 1158
RsaNI GTAC 1 cut(s) 1157
RseI CAYNNNNRTG 3 cut(s) 538, 1234, 1710
SacI GAGCTC 1 cut(s) 156
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 6 cut(s) 603, 795, 1122, 1526, 1613, 1737
SatI GCNGC 7 cut(s) 87, 90, 160, 171, 174, 975, 1857
Sau3AI GATC 5 cut(s) 566, 832, 1270, 1559, 1918
Sau96I GGNCC 4 cut(s) 1082, 1323, 1453, 1799
ScaI AGTACT 1 cut(s) 1158
SchI GAGTC 5 cut(s) 219, 227, 275, 329, 923
ScrFI CCNGG 2 cut(s) 23, 1397
SduI GDGCHC 3 cut(s) 46, 58, 156
SfaNI GCATC 6 cut(s) 580, 741, 1238, 1357, 1671, 1825
SfcI CTRYAG 2 cut(s) 1402, 1719
SfuI TTCGAA 1 cut(s) 899
SinI GGWCC 3 cut(s) 1082, 1323, 1799
SmiMI CAYNNNNRTG 3 cut(s) 538, 1234, 1710
SmlI CTYRAG 2 cut(s) 269, 1637
SmoI CTYRAG 2 cut(s) 269, 1637
SpeI ACTAGT 1 cut(s) 880
SseBI AGGCCT 1 cut(s) 1400
SspI AATATT 1 cut(s) 1174
SspMI CTAG 5 cut(s) 513, 881, 1412, 1982, 2133
SstI GAGCTC 1 cut(s) 156
StuI AGGCCT 1 cut(s) 1400
StyD4I CCNGG 2 cut(s) 21, 1395
StyI CCWWGG 2 cut(s) 237, 963
TaiI ACGT 7 cut(s) 43, 55, 120, 168, 261, 391, 1582
TaqI TCGA 9 cut(s) 128, 135, 192, 198, 246, 405, 471, 899, 1962
TatI WGTACW 1 cut(s) 1156
TauI GCSGC 5 cut(s) 89, 92, 162, 173, 176
TfiI GAWTC 6 cut(s) 705, 802, 896, 2017, 2078, 2117
Tru1I TTAA 6 cut(s) 603, 795, 1122, 1526, 1613, 1737
Tru9I TTAA 6 cut(s) 603, 795, 1122, 1526, 1613, 1737
TscAI CASTG 5 cut(s) 540, 631, 1257, 1273, 1728
TseFI GTSAC 6 cut(s) 36, 48, 118, 211, 915, 1034
TseI GCWGC 2 cut(s) 974, 1856
Tsp45I GTSAC 6 cut(s) 36, 48, 118, 211, 915, 1034
TspGWI ACGGA 2 cut(s) 646, 1204
TspRI CASTG 5 cut(s) 540, 631, 1257, 1273, 1728
VpaK11BI GGWCC 3 cut(s) 1082, 1323, 1799
XagI CCTNNNNNAGG 1 cut(s) 1824
XapI RAATTY 6 cut(s) 862, 1073, 1106, 1245, 1336, 1881
XbaI TCTAGA 2 cut(s) 512, 2132
XceI RCATGY 2 cut(s) 1434, 2094
XmiI GTMKAC 3 cut(s) 68, 192, 487
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 5 cut(s) 513, 881, 1412, 1982, 2133
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 1158
Zsp2I ATGCAT 1 cut(s) 1664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.