Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0004536

Physical Location & Seq

Genomic Coordinates Forward (+)

36416769 .. 36417955

Gene Structure 1187 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g43731.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGCTTCACAATCAAGATGGACGAAACCGGTACCAGAAAAAGAAGTGCTCCGCCGATTAATTGCCGAACACAGAAAACTGTGCACAAGTCAGAAGGCTAATTCTGCGACCTCTTCTAGACATGAAGATGTTGATTATCACGAGGCCAAAGACCTCTCTATCACGGAAATAAAGGAGGAACTAGAAGAGCTCAAGCAGAAATTAGATGATCCGGACTTTAGTTTGGTTACACGAGGCGATCGTAACGTGACCTATCGTGCTTGCAGTGATGGGGTCCGACGTCTTACAGTGAAAAATATAAATACACGCTCTAAACCAAAAGATGTTAAAAGAAACGATGTGAGTGATGTGATTGATTATATTCTCAAATCCATCGAAGAAAACATGAGGGAAACACATCAAGGGGGGTATTACCAAAAAAGAAATGGCGATAAAAAGTTCAAGAAAGAACAAATGTACGACATTATTGATGATATATGTTGCACTATAGGGTGCACAATTCCAAGTTTCCCCGTAGATGCTGCTGAAAAAGCTTTTGTTGGAGGTCGGCTTTCGTGGATGCAGAATGGTTACCCAACCTATTGTCAAAGCCGAGATGATGGGGTGGCCGTCCCTAATTTGCGTTCAAAGATGATTTGGAGAATCATGAAGCCAAGCATGGGAATGCCCGGGGTGGGGGGCAGAAGATTTATAAAGCTGTTGTGGGAGACTTTCAGGCTTCCCACGTATGGCCTTTTCGATTGTGATCCATCAGGTATCATAATATTCAACACTTATACTGTTGGGTCGATTGAAAAGGCCTACGACAATGTAAGTTTAACTTGTCCGTCGATGACATGGTTAGGTGTTTGGCCGAGTGACCTAAGAGACCTTGAGATAGCCGGGGAGGATTTGAGTGAAAAGAATATTCAAGAAGTAGACAATCTTTTGAAGAAAGAATTTGTTAAAGATAATCCGAGGTTGAAAGAAGAGCTGAAAGAAATGAAAAGGACAGGGAAAAAAGCCAACCTGGAAGCTTTGAAGGAGAAATTTGGACCCGCTTGGTTGTCAAAGGGCTTTTTGCCGTATAAGTTGCATCGTCTTTTGACCGTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030957 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046983 GO:0048285 GO:0050896 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

366

Amino Acids

41.99

Weight (kDa)

8.89

Isoelectric Point (pI)

51.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP6A-Spo11_Toprim	PF21180	219 - 358	1.3e-34	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004536)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43731 FvH4_3g43753
rosa_chinensis	RchiOBHm_Chr2g0086091 RchiOBHm_Chr2g0089291 RchiOBHm_Chr2g0089851 RchiOBHm_Chr5g0083311
rosa_laevigata	RLG00000015741 RLG00000016061 RLG00000017209
rosa_multiflora	Rmu_co8293021.1_g000001 Rmu_co8360049.1_g000001 Rmu_sc0000388.1_g000047 Rmu_sc0002384.1_g000009 Rmu_sc0003382.1_g000021 Rmu_sc0017765.1_g000002 Rmu_ssc0000355.1_g000016
rosa_roxburghii	Rroxscaffold_2G00140180 Rroxscaffold_2G00151540
rosa_rugosa	Rorug01G0465600 Rorug01G0488800 Rorug02G0003700
rosa_samantha	Rh2AG015000 Rh2AG045100 Rh2AG048900 Rh2BG047800 Rh2CG016000 Rh2CG016100 Rh2CG045000 Rh2CG049900 Rh5BG561000 Rh5CG583600
rosa_wichuraiana	Rw2G001320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	692
AatII	GACGTC	1 cut(s)	283
Acc65I	GGTACC	1 cut(s)	31
AccB1I	GGYRCC	1 cut(s)	31
AccI	GTMKAC	1 cut(s)	918
AccIII	TCCGGA	1 cut(s)	211
AciI	CCGC	2 cut(s)	52, 1038
AclWI	GGATC	2 cut(s)	203, 740
AcoI	YGGCCR	2 cut(s)	606, 851
AcsI	RAATTY	2 cut(s)	938, 1028
AcyI	GRCGYC	1 cut(s)	280
AfaI	GTAC	2 cut(s)	33, 458
AfiI	CCNNNNNNNGG	4 cut(s)	659, 674, 675, 728
AgeI	ACCGGT	1 cut(s)	28
AgsI	TTSAA	8 cut(s)	442, 627, 769, 794, 911, 931, 964, 1021
AjnI	CCWGG	1 cut(s)	1008
AluBI	AGCT	5 cut(s)	190, 533, 697, 973, 1016
AluI	AGCT	5 cut(s)	190, 533, 697, 973, 1016
Alw21I	GWGCWC	4 cut(s)	51, 86, 192, 497
Alw26I	GTCTC	2 cut(s)	701, 861
Alw44I	GTGCAC	2 cut(s)	82, 493
AlwI	GGATC	2 cut(s)	203, 740
Ama87I	CYCGRG	1 cut(s)	668
Aor13HI	TCCGGA	1 cut(s)	211
AoxI	GGCC	5 cut(s)	144, 606, 730, 798, 851
ApaLI	GTGCAC	2 cut(s)	82, 493
ApeKI	GCWGC	1 cut(s)	521
ApoI	RAATTY	2 cut(s)	938, 1028
AseI	ATTAAT	1 cut(s)	59
AsiGI	ACCGGT	1 cut(s)	28
Asp718I	GGTACC	1 cut(s)	31
AspS9I	GGNCC	2 cut(s)	274, 1034
AsuC2I	CCSGG	3 cut(s)	669, 670, 883
AvaI	CYCGRG	1 cut(s)	668
AvaII	GGWCC	2 cut(s)	274, 1034
BaeGI	GKGCMC	2 cut(s)	86, 497
BanI	GGYRCC	1 cut(s)	31
BanII	GRGCYC	1 cut(s)	192
BauI	CACGAG	2 cut(s)	140, 231
Bbv12I	GWGCWC	4 cut(s)	51, 86, 192, 497
BbvI	GCAGC	1 cut(s)	508
BccI	CCATC	5 cut(s)	12, 263, 380, 593, 757
BceAI	ACGGC	2 cut(s)	593, 1048
BciT130I	CCWGG	1 cut(s)	1010
BcnI	CCSGG	3 cut(s)	669, 670, 883
BcoDI	GTCTC	2 cut(s)	701, 861
BfaI	CTAG	2 cut(s)	117, 182
BfmI	CTRYAG	1 cut(s)	486
BisI	GCNGC	1 cut(s)	522
BlsI	GCNGC	1 cut(s)	523
Bme1390I	CCNGG	4 cut(s)	669, 670, 883, 1010
Bme18I	GGWCC	2 cut(s)	274, 1034
BmeT110I	CYCGRG	1 cut(s)	668
BmgT120I	GGNCC	2 cut(s)	274, 1034
BmiI	GGNNCC	3 cut(s)	33, 275, 1036
BmrFI	CCNGG	4 cut(s)	669, 670, 883, 1010
BmsI	GCATC	3 cut(s)	508, 549, 1084
BpuEI	CTTGAG	2 cut(s)	176, 893
BpuMI	CCSGG	3 cut(s)	669, 670, 883
BsaAI	YACGTR	1 cut(s)	726
BsaBI	GATNNNNATC	1 cut(s)	744
BsaHI	GRCGYC	1 cut(s)	280
BsaI	GGTCTC	1 cut(s)	861
BsaJI	CCNNGG	4 cut(s)	668, 669, 882, 956
BsaWI	WCCGGW	2 cut(s)	28, 211
Bsc4I	CCNNNNNNNGG	4 cut(s)	659, 674, 675, 728
Bse118I	RCCGGY	1 cut(s)	28
Bse8I	GATNNNNATC	1 cut(s)	744
BseAI	TCCGGA	1 cut(s)	211
BseBI	CCWGG	1 cut(s)	1010
BseDI	CCNNGG	4 cut(s)	668, 669, 882, 956
BseGI	GGATG	1 cut(s)	564
BseJI	GATNNNNATC	1 cut(s)	744
BseLI	CCNNNNNNNGG	4 cut(s)	659, 674, 675, 728
BseSI	GKGCMC	2 cut(s)	86, 497
BseXI	GCAGC	1 cut(s)	508
Bsh1285I	CGRYCG	1 cut(s)	241
BshFI	GGCC	5 cut(s)	146, 608, 732, 800, 853
BshNI	GGYRCC	1 cut(s)	31
BshTI	ACCGGT	1 cut(s)	28
BsiEI	CGRYCG	1 cut(s)	241
BsiHKAI	GWGCWC	4 cut(s)	51, 86, 192, 497
BsiHKCI	CYCGRG	1 cut(s)	668
BsiSI	CCGG	4 cut(s)	29, 212, 669, 882
BslFI	GGGAC	1 cut(s)	596
BslI	CCNNNNNNNGG	4 cut(s)	659, 674, 675, 728
BsmAI	GTCTC	2 cut(s)	701, 861
BsmFI	GGGAC	1 cut(s)	596
BsmI	GAATGC	1 cut(s)	669
BsnI	GGCC	5 cut(s)	146, 608, 732, 800, 853
Bso31I	GGTCTC	1 cut(s)	861
BsoBI	CYCGRG	1 cut(s)	668
Bsp1286I	GDGCHC	4 cut(s)	51, 86, 192, 497
Bsp13I	TCCGGA	1 cut(s)	211
Bsp143I	GATC	3 cut(s)	208, 238, 745
BspACI	CCGC	2 cut(s)	52, 1038
BspANI	GGCC	5 cut(s)	146, 608, 732, 800, 853
BspEI	TCCGGA	1 cut(s)	211
BspHI	TCATGA	1 cut(s)	645
BspLI	GGNNCC	3 cut(s)	33, 275, 1036
BspPI	GGATC	2 cut(s)	203, 740
BspQI	GCTCTTC	2 cut(s)	180, 963
BspT107I	GGYRCC	1 cut(s)	31
BspTNI	GGTCTC	1 cut(s)	861
BsrFI	RCCGGY	1 cut(s)	28
BssAI	RCCGGY	1 cut(s)	28
BssECI	CCNNGG	4 cut(s)	668, 669, 882, 956
BssMI	GATC	3 cut(s)	208, 238, 745
BssNI	GRCGYC	1 cut(s)	280
BssSI	CACGAG	2 cut(s)	140, 231
Bst2BI	CACGAG	2 cut(s)	140, 231
Bst2UI	CCWGG	1 cut(s)	1010
Bst4CI	ACNGT	4 cut(s)	81, 289, 781, 1090
Bst6I	CTCTTC	3 cut(s)	118, 180, 963
BstACI	GRCGYC	1 cut(s)	280
BstBAI	YACGTR	1 cut(s)	726
BstC8I	GCNNGC	1 cut(s)	262
BstDEI	CTNAG	1 cut(s)	863
BstEII	GGTNACC	1 cut(s)	569
BstF5I	GGATG	1 cut(s)	564
BstKTI	GATC	3 cut(s)	211, 241, 748
BstMAI	GTCTC	2 cut(s)	701, 861
BstMBI	GATC	3 cut(s)	208, 238, 745
BstMCI	CGRYCG	1 cut(s)	241
BstMWI	GCNNNNNNNGC	2 cut(s)	104, 530
BstNI	CCWGG	1 cut(s)	1010
BstPI	GGTNACC	1 cut(s)	569
BstSCI	CCNGG	4 cut(s)	667, 668, 881, 1008
BstSFI	CTRYAG	1 cut(s)	486
BstSLI	GKGCMC	2 cut(s)	86, 497
BstV1I	GCAGC	1 cut(s)	508
BsuRI	GGCC	5 cut(s)	146, 608, 732, 800, 853
BtsCI	GGATG	1 cut(s)	564
BtsI	GCAGTG	1 cut(s)	271
BtsIMutI	CAGTG	2 cut(s)	271, 294
Cac8I	GCNNGC	1 cut(s)	262
CciI	TCATGA	1 cut(s)	645
Cfr10I	RCCGGY	1 cut(s)	28
Cfr13I	GGNCC	2 cut(s)	274, 1034
Cfr9I	CCCGGG	1 cut(s)	668
Csp6I	GTAC	2 cut(s)	32, 457
CspAI	ACCGGT	1 cut(s)	28
CviAII	CATG	5 cut(s)	122, 385, 646, 658, 837
CviQI	GTAC	2 cut(s)	32, 457
DdeI	CTNAG	1 cut(s)	863
DpnI	GATC	3 cut(s)	210, 240, 747
DpnII	GATC	3 cut(s)	208, 238, 745
EaeI	YGGCCR	2 cut(s)	606, 851
Eam1104I	CTCTTC	3 cut(s)	118, 180, 963
EarI	CTCTTC	3 cut(s)	118, 180, 963
EciI	GGCGGA	1 cut(s)	41
Ecl136II	GAGCTC	1 cut(s)	190
Eco147I	AGGCCT	1 cut(s)	800
Eco24I	GRGCYC	1 cut(s)	192
Eco31I	GGTCTC	1 cut(s)	861
Eco47I	GGWCC	2 cut(s)	274, 1034
Eco53kI	GAGCTC	1 cut(s)	190
Eco88I	CYCGRG	1 cut(s)	668
Eco91I	GGTNACC	1 cut(s)	569
EcoICRI	GAGCTC	1 cut(s)	190
EcoO65I	GGTNACC	1 cut(s)	569
EcoRII	CCWGG	1 cut(s)	1008
EcoT38I	GRGCYC	1 cut(s)	192
FaeI	CATG	5 cut(s)	125, 388, 649, 661, 840
FalI	AAGNNNNNCTT	2 cut(s)	805, 837
FaqI	GGGAC	1 cut(s)	596
FatI	CATG	5 cut(s)	121, 384, 645, 657, 836
FauI	CCCGC	1 cut(s)	1045
FblI	GTMKAC	1 cut(s)	918
Fnu4HI	GCNGC	1 cut(s)	522
FokI	GGATG	1 cut(s)	571
FriOI	GRGCYC	1 cut(s)	192
Fsp4HI	GCNGC	1 cut(s)	522
FspBI	CTAG	2 cut(s)	117, 182
GluI	GCNGC	1 cut(s)	522
HaeIII	GGCC	5 cut(s)	146, 608, 732, 800, 853
HapII	CCGG	4 cut(s)	29, 212, 669, 882
Hin1I	GRCGYC	1 cut(s)	280
Hin1II	CATG	5 cut(s)	125, 388, 649, 661, 840
HindIII	AAGCTT	2 cut(s)	531, 1014
HinfI	GANTC	1 cut(s)	642
HpaII	CCGG	4 cut(s)	29, 212, 669, 882
Hpy166II	GTNNAC	3 cut(s)	84, 495, 919
Hpy188I	TCNGA	3 cut(s)	93, 278, 957
Hpy188III	TCNNGA	7 cut(s)	15, 117, 140, 212, 442, 646, 911
Hpy8I	GTNNAC	3 cut(s)	84, 495, 919
Hpy99I	CGWCG	2 cut(s)	282, 832
HpyAV	CCTTC	2 cut(s)	88, 1015
HpyCH4III	ACNGT	4 cut(s)	81, 289, 781, 1090
HpyCH4IV	ACGT	3 cut(s)	246, 280, 725
HpyCH4V	TGCA	6 cut(s)	84, 264, 483, 495, 562, 1075
HpyF10VI	GCNNNNNNNGC	2 cut(s)	104, 530
HpyF3I	CTNAG	1 cut(s)	863
HpySE526I	ACGT	3 cut(s)	246, 280, 725
Hsp92I	GRCGYC	1 cut(s)	280
Hsp92II	CATG	5 cut(s)	125, 388, 649, 661, 840
Kpn2I	TCCGGA	1 cut(s)	211
KpnI	GGTACC	1 cut(s)	35
Kzo9I	GATC	3 cut(s)	208, 238, 745
LguI	GCTCTTC	2 cut(s)	180, 963
LmnI	GCTCC	1 cut(s)	54
Lsp1109I	GCAGC	1 cut(s)	508
LweI	GCATC	3 cut(s)	508, 549, 1084
MaeI	CTAG	2 cut(s)	117, 182
MaeII	ACGT	3 cut(s)	246, 280, 725
MaeIII	GTNAC	5 cut(s)	226, 242, 247, 569, 857
MalI	GATC	3 cut(s)	210, 240, 747
MboI	GATC	3 cut(s)	208, 238, 745
MboII	GAAGA	7 cut(s)	105, 137, 197, 389, 696, 943, 980
MhlI	GDGCHC	4 cut(s)	51, 86, 192, 497
MluCI	AATT	7 cut(s)	60, 100, 200, 498, 616, 938, 1028
MmeI	TCCRAC	2 cut(s)	301, 520
MnlI	CCTC	9 cut(s)	121, 136, 164, 169, 227, 381, 536, 880, 951
MroI	TCCGGA	1 cut(s)	211
MseI	TTAA	4 cut(s)	59, 327, 818, 945
MslI	CAYNNNNRTG	2 cut(s)	126, 662
MspI	CCGG	4 cut(s)	29, 212, 669, 882
MspR9I	CCNGG	4 cut(s)	669, 670, 883, 1010
Mva1269I	GAATGC	1 cut(s)	669
MvaI	CCWGG	1 cut(s)	1010
MwoI	GCNNNNNNNGC	2 cut(s)	104, 530
NciI	CCSGG	3 cut(s)	669, 670, 883
NdeII	GATC	3 cut(s)	208, 238, 745
NlaIII	CATG	5 cut(s)	125, 388, 649, 661, 840
NlaIV	GGNNCC	3 cut(s)	33, 275, 1036
NmeAIII	GCCGAG	2 cut(s)	617, 879
NmuCI	GTSAC	2 cut(s)	247, 857
PagI	TCATGA	1 cut(s)	645
PceI	AGGCCT	1 cut(s)	800
PciSI	GCTCTTC	2 cut(s)	180, 963
PcsI	WCGNNNNNNNCGW	1 cut(s)	238
PctI	GAATGC	1 cut(s)	669
PfeI	GAWTC	1 cut(s)	642
PinAI	ACCGGT	1 cut(s)	28
PkrI	GCNGC	1 cut(s)	523
Ple19I	CGATCG	1 cut(s)	241
Ppu21I	YACGTR	1 cut(s)	726
PshBI	ATTAAT	1 cut(s)	59
PsiI	TTATAA	1 cut(s)	692
Psp124BI	GAGCTC	1 cut(s)	192
Psp6I	CCWGG	1 cut(s)	1008
PspEI	GGTNACC	1 cut(s)	569
PspGI	CCWGG	1 cut(s)	1008
PspN4I	GGNNCC	3 cut(s)	33, 275, 1036
PspPI	GGNCC	2 cut(s)	274, 1034
PvuI	CGATCG	1 cut(s)	241
RsaI	GTAC	2 cut(s)	33, 458
RsaNI	GTAC	2 cut(s)	32, 457
RseI	CAYNNNNRTG	2 cut(s)	126, 662
SacI	GAGCTC	1 cut(s)	192
SapI	GCTCTTC	2 cut(s)	180, 963
SaqAI	TTAA	4 cut(s)	59, 327, 818, 945
SatI	GCNGC	1 cut(s)	522
Sau3AI	GATC	3 cut(s)	208, 238, 745
Sau96I	GGNCC	2 cut(s)	274, 1034
ScrFI	CCNGG	4 cut(s)	669, 670, 883, 1010
SduI	GDGCHC	4 cut(s)	51, 86, 192, 497
SfaNI	GCATC	3 cut(s)	508, 549, 1084
SfcI	CTRYAG	1 cut(s)	486
SinI	GGWCC	2 cut(s)	274, 1034
SmaI	CCCGGG	1 cut(s)	670
SmiMI	CAYNNNNRTG	2 cut(s)	126, 662
SmlI	CTYRAG	2 cut(s)	191, 872
SmoI	CTYRAG	2 cut(s)	191, 872
Sse9I	AATT	7 cut(s)	60, 100, 200, 498, 616, 938, 1028
SseBI	AGGCCT	1 cut(s)	800
SsiI	CCGC	2 cut(s)	52, 1038
SspI	AATATT	2 cut(s)	765, 907
SspMI	CTAG	2 cut(s)	117, 182
SstI	GAGCTC	1 cut(s)	192
StuI	AGGCCT	1 cut(s)	800
StyD4I	CCNGG	4 cut(s)	667, 668, 881, 1008
TaaI	ACNGT	4 cut(s)	81, 289, 781, 1090
TaiI	ACGT	3 cut(s)	249, 283, 728
TaqI	TCGA	4 cut(s)	375, 738, 788, 830
TasI	AATT	7 cut(s)	60, 100, 200, 498, 616, 938, 1028
TfiI	GAWTC	1 cut(s)	642
Tru1I	TTAA	4 cut(s)	59, 327, 818, 945
Tru9I	TTAA	4 cut(s)	59, 327, 818, 945
TscAI	CASTG	2 cut(s)	271, 294
TseFI	GTSAC	2 cut(s)	247, 857
TseI	GCWGC	1 cut(s)	521
Tsp45I	GTSAC	2 cut(s)	247, 857
TspDTI	ATGAA	3 cut(s)	138, 662, 998
TspGWI	ACGGA	2 cut(s)	179, 816
TspMI	CCCGGG	1 cut(s)	668
TspRI	CASTG	2 cut(s)	271, 294
VneI	GTGCAC	2 cut(s)	82, 493
VpaK11BI	GGWCC	2 cut(s)	274, 1034
VspI	ATTAAT	1 cut(s)	59
XapI	RAATTY	2 cut(s)	938, 1028
XbaI	TCTAGA	1 cut(s)	116
XcmI	CCANNNNNNNNNTGG	1 cut(s)	422
XmaI	CCCGGG	1 cut(s)	668
XmiI	GTMKAC	1 cut(s)	918
XspI	CTAG	2 cut(s)	117, 182
ZraI	GACGTC	1 cut(s)	281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.