Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0004536

Physical Location & Seq

Genomic Coordinates Reverse (-)

78424705 .. 78425877

Gene Structure 1173 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00140180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGCAGCCTCTGCCTCTCACCTGGCAAGTACCAGATCAAGAGCATGTCCTCCAAGGACTGCGTGATTTTCTTAGCTGGTATCGCAGAAATAATCAGAGAATGTCCTCCACCGCCTCCTCTTTGAATCCAGAATGTTCCAAATTTCAAGTTGAAGACCTCTCTCTTGACGATATAGCAGTGAGGATTCGAGATTTTAAAAAGGACATATTAGAGGGTAAGGAACTACATTTGATTTCCAGAGGTTGCTCCAATACCATTTTTTCCGGCGATCGTATTTTACTAAAGGATCAAGGTGTTCGTTATAAACCAAGGGATCGTAAGAGAGACATCATCCTTCGTCTTCTTGACATTATTCTCATGTTTTGCAATGGACATACCCGCTACACACTTCGCAGCCTTCGCTCGAGTCTCAACGTGTTTGCTTCTGACAGAGGCTATGTAGGAGGTGTCCTCACTTTTAGGCTAGATGGTCAAGAATTGGAAATGGGACAATATGGCCAGGCGATCCCTCCTTTTATTGATGATCAGCTTACTGATGTACAGACTCACGGTGCACTGTTCATACTATTTGTGGAGAAACATACTGCATATGTTAGCTTGATGGAGCACAAATTTTACCTGGACTATCCCTGCATCATAATCACTGGAATGGGAGTTCCTGATGTCGCAACAAGGCGCTTCATTAACTACTTGTCAACCACCTTCGAACTCCCAACCTTTGCCATTGTGGATAGTGATCCATATGGATTCCAAATTCTATCCACGTGTGCTTTTGGCTCCGAAGCTATGTGTTATGATAATGAAAAGTTGGCTACCCCAGGTATCATATGGCTGGGTCTCTTACCTAGTGATTTGGAGGCGCTTAAAATCCCCAGTCAGGATATGAGTGTACAGGAAATCAAAAAGGTACATGATCTTATGAAGAAAGATTACGTGCAGAGACATGAACCCTGGTGGGAAGAGCTGAATGATATGTGCCGGACCAAAAAAGTGGCAAACATTGATGTTTTAAATAACTTCGGGGTCCATACTCTTGCCAAATCTCTGTTGCCATACAAATTGAATCACTTTTCAAAATTGCCAGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0008544 GO:0009888 GO:0009913 GO:0009957 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030154 GO:0030855 GO:0031974 GO:0031981 GO:0032502 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045165 GO:0046483 GO:0048285 GO:0048856 GO:0048869 GO:0050896 GO:0051321 GO:0051716 GO:0060429 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

362

Amino Acids

41.62

Weight (kDa)

6.37

Isoelectric Point (pI)

34.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP6A-Spo11_Toprim	PF21180	187 - 354	1.7e-49	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004536)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43731 FvH4_3g43753
rosa_chinensis	RchiOBHm_Chr2g0086091 RchiOBHm_Chr2g0089291 RchiOBHm_Chr2g0089851 RchiOBHm_Chr5g0083311
rosa_laevigata	RLG00000015741 RLG00000016061 RLG00000017209
rosa_multiflora	Rmu_co8293021.1_g000001 Rmu_co8360049.1_g000001 Rmu_sc0000388.1_g000047 Rmu_sc0002384.1_g000009 Rmu_sc0003382.1_g000021 Rmu_sc0017765.1_g000002 Rmu_ssc0000355.1_g000016
rosa_roxburghii	Rroxscaffold_2G00140180 Rroxscaffold_2G00151540
rosa_rugosa	Rorug01G0465600 Rorug01G0488800 Rorug02G0003700
rosa_samantha	Rh2AG015000 Rh2AG045100 Rh2AG048900 Rh2BG047800 Rh2CG016000 Rh2CG016100 Rh2CG045000 Rh2CG049900 Rh5BG561000 Rh5CG583600
rosa_wichuraiana	Rw2G001320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	303
AciI	CCGC	2 cut(s)	111, 379
AclWI	GGATC	4 cut(s)	294, 321, 499, 731
AcoI	YGGCCR	1 cut(s)	496
AcsI	RAATTY	3 cut(s)	140, 611, 753
AcvI	CACGTG	1 cut(s)	765
AfaI	GTAC	4 cut(s)	30, 540, 891, 909
AfiI	CCNNNNNNNGG	1 cut(s)	877
AflIII	ACRYGT	2 cut(s)	414, 764
AgsI	TTSAA	5 cut(s)	124, 146, 152, 1063, 1074
AjnI	CCWGG	6 cut(s)	20, 498, 618, 817, 950, 1081
AloI	GAACNNNNNNTCC	2 cut(s)	639, 671
AluBI	AGCT	5 cut(s)	75, 529, 597, 785, 964
AluI	AGCT	5 cut(s)	75, 529, 597, 785, 964
Alw21I	GWGCWC	2 cut(s)	556, 609
Alw26I	GTCTC	4 cut(s)	318, 413, 842, 934
Alw44I	GTGCAC	1 cut(s)	552
AlwI	GGATC	4 cut(s)	294, 321, 499, 731
AlwNI	CAGNNNCTG	1 cut(s)	10
Ama87I	CYCGRG	1 cut(s)	403
AoxI	GGCC	1 cut(s)	496
ApaLI	GTGCAC	1 cut(s)	552
ApeKI	GCWGC	2 cut(s)	4, 393
ApoI	RAATTY	3 cut(s)	140, 611, 753
AspLEI	GCGC	2 cut(s)	678, 862
AspS9I	GGNCC	2 cut(s)	981, 1024
AsuHPI	GGTGA	1 cut(s)	10
AsuII	TTCGAA	1 cut(s)	705
AvaI	CYCGRG	1 cut(s)	403
AvaII	GGWCC	2 cut(s)	981, 1024
BaeGI	GKGCMC	1 cut(s)	556
BalI	TGGCCA	1 cut(s)	498
BarI	GAAGNNNNNNTAC	2 cut(s)	914, 946
BbrPI	CACGTG	1 cut(s)	765
BbsI	GAAGAC	2 cut(s)	159, 332
Bbv12I	GWGCWC	2 cut(s)	556, 609
BbvI	GCAGC	2 cut(s)	16, 405
BccI	CCATC	2 cut(s)	461, 595
BciT130I	CCWGG	6 cut(s)	22, 500, 620, 819, 952, 1083
BclI	TGATCA	1 cut(s)	523
BcoDI	GTCTC	4 cut(s)	318, 413, 842, 934
BfaI	CTAG	2 cut(s)	464, 846
BfoI	RGCGCY	2 cut(s)	679, 863
BisI	GCNGC	2 cut(s)	5, 394
BlsI	GCNGC	2 cut(s)	6, 395
Bme1390I	CCNGG	6 cut(s)	22, 500, 620, 819, 952, 1083
Bme18I	GGWCC	2 cut(s)	981, 1024
BmeT110I	CYCGRG	1 cut(s)	403
BmgT120I	GGNCC	2 cut(s)	981, 1024
BmiI	GGNNCC	2 cut(s)	778, 1025
BmrFI	CCNGG	6 cut(s)	22, 500, 620, 819, 952, 1083
BmrI	ACTGGG	1 cut(s)	867
BmsI	GCATC	1 cut(s)	642
BmuI	ACTGGG	1 cut(s)	867
BpiI	GAAGAC	2 cut(s)	159, 332
BplI	GAGNNNNNCTC	2 cut(s)	435, 467
Bpu14I	TTCGAA	1 cut(s)	705
BsaAI	YACGTR	2 cut(s)	765, 934
BsaBI	GATNNNNATC	1 cut(s)	735
BsaI	GGTCTC	1 cut(s)	842
BsaJI	CCNNGG	4 cut(s)	52, 308, 817, 950
Bsc4I	CCNNNNNNNGG	1 cut(s)	877
Bse1I	ACTGG	2 cut(s)	649, 873
Bse3DI	GCAATG	1 cut(s)	373
Bse8I	GATNNNNATC	1 cut(s)	735
BseBI	CCWGG	6 cut(s)	22, 500, 620, 819, 952, 1083
BseDI	CCNNGG	4 cut(s)	52, 308, 817, 950
BseGI	GGATG	1 cut(s)	330
BseJI	GATNNNNATC	1 cut(s)	735
BseLI	CCNNNNNNNGG	1 cut(s)	877
BseMI	GCAATG	1 cut(s)	373
BseNI	ACTGG	2 cut(s)	649, 873
BseRI	GAGGAG	1 cut(s)	106
BseSI	GKGCMC	1 cut(s)	556
BseXI	GCAGC	2 cut(s)	16, 405
BseYI	CCCAGC	1 cut(s)	832
BsgI	GTGCAG	1 cut(s)	956
Bsh1285I	CGRYCG	1 cut(s)	271
BshFI	GGCC	1 cut(s)	498
BsiEI	CGRYCG	1 cut(s)	271
BsiHKAI	GWGCWC	2 cut(s)	556, 609
BsiHKCI	CYCGRG	1 cut(s)	403
BsiSI	CCGG	2 cut(s)	264, 979
BslFI	GGGAC	1 cut(s)	501
BslI	CCNNNNNNNGG	1 cut(s)	877
BsmAI	GTCTC	4 cut(s)	318, 413, 842, 934
BsmFI	GGGAC	1 cut(s)	501
BsnI	GGCC	1 cut(s)	498
Bso31I	GGTCTC	1 cut(s)	842
BsoBI	CYCGRG	1 cut(s)	403
Bsp119I	TTCGAA	1 cut(s)	705
Bsp1286I	GDGCHC	2 cut(s)	556, 609
Bsp1407I	TGTACA	2 cut(s)	538, 889
Bsp143I	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
BspACI	CCGC	2 cut(s)	111, 379
BspANI	GGCC	1 cut(s)	498
BspLI	GGNNCC	2 cut(s)	778, 1025
BspPI	GGATC	4 cut(s)	294, 321, 499, 731
BspQI	GCTCTTC	1 cut(s)	954
BspT104I	TTCGAA	1 cut(s)	705
BspTNI	GGTCTC	1 cut(s)	842
BsrDI	GCAATG	1 cut(s)	373
BsrGI	TGTACA	2 cut(s)	538, 889
BsrI	ACTGG	2 cut(s)	649, 873
BssECI	CCNNGG	4 cut(s)	52, 308, 817, 950
BssMI	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
BssT1I	CCWWGG	2 cut(s)	52, 308
Bst2UI	CCWGG	6 cut(s)	22, 500, 620, 819, 952, 1083
Bst4CI	ACNGT	2 cut(s)	551, 558
Bst6I	CTCTTC	1 cut(s)	954
BstAPI	GCANNNNNTGC	1 cut(s)	10
BstAUI	TGTACA	2 cut(s)	538, 889
BstBAI	YACGTR	2 cut(s)	765, 934
BstBI	TTCGAA	1 cut(s)	705
BstDEI	CTNAG	1 cut(s)	71
BstF5I	GGATG	1 cut(s)	330
BstH2I	RGCGCY	2 cut(s)	679, 863
BstHHI	GCGC	2 cut(s)	678, 862
BstKTI	GATC	8 cut(s)	37, 271, 289, 316, 507, 526, 739, 916
BstMAI	GTCTC	4 cut(s)	318, 413, 842, 934
BstMBI	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
BstMCI	CGRYCG	1 cut(s)	271
BstMWI	GCNNNNNNNGC	3 cut(s)	10, 81, 399
BstNI	CCWGG	6 cut(s)	22, 500, 620, 819, 952, 1083
BstNSI	RCATGY	1 cut(s)	47
BstSCI	CCNGG	6 cut(s)	20, 498, 618, 817, 950, 1081
BstSLI	GKGCMC	1 cut(s)	556
BstV1I	GCAGC	2 cut(s)	16, 405
BstV2I	GAAGAC	2 cut(s)	159, 332
BstXI	CCANNNNNNTGG	1 cut(s)	991
BsuRI	GGCC	1 cut(s)	498
BtsCI	GGATG	1 cut(s)	330
BtsI	GCAGTG	1 cut(s)	183
BtsIMutI	CAGTG	3 cut(s)	183, 554, 642
CaiI	CAGNNNCTG	1 cut(s)	10
CfoI	GCGC	2 cut(s)	678, 862
Cfr13I	GGNCC	2 cut(s)	981, 1024
Csp6I	GTAC	4 cut(s)	29, 539, 890, 908
CviAII	CATG	4 cut(s)	44, 358, 911, 944
CviQI	GTAC	4 cut(s)	29, 539, 890, 908
DdeI	CTNAG	1 cut(s)	71
DpnI	GATC	8 cut(s)	36, 270, 288, 315, 506, 525, 738, 915
DpnII	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
DraI	TTTAAA	2 cut(s)	196, 1011
EaeI	YGGCCR	1 cut(s)	496
Eam1104I	CTCTTC	1 cut(s)	954
EarI	CTCTTC	1 cut(s)	954
Eco130I	CCWWGG	2 cut(s)	52, 308
Eco31I	GGTCTC	1 cut(s)	842
Eco47I	GGWCC	2 cut(s)	981, 1024
Eco72I	CACGTG	1 cut(s)	765
Eco88I	CYCGRG	1 cut(s)	403
EcoRII	CCWGG	6 cut(s)	20, 498, 618, 817, 950, 1081
EcoT14I	CCWWGG	2 cut(s)	52, 308
ErhI	CCWWGG	2 cut(s)	52, 308
FaeI	CATG	4 cut(s)	47, 361, 914, 947
FaqI	GGGAC	1 cut(s)	501
FatI	CATG	4 cut(s)	43, 357, 910, 943
FauI	CCCGC	1 cut(s)	386
FauNDI	CATATG	3 cut(s)	589, 742, 827
FbaI	TGATCA	1 cut(s)	523
Fnu4HI	GCNGC	2 cut(s)	5, 394
FokI	GGATG	1 cut(s)	317
Fsp4HI	GCNGC	2 cut(s)	5, 394
FspBI	CTAG	2 cut(s)	464, 846
GlaI	GCGC	2 cut(s)	677, 861
GluI	GCNGC	2 cut(s)	5, 394
GsaI	CCCAGC	1 cut(s)	836
HaeII	RGCGCY	2 cut(s)	679, 863
HaeIII	GGCC	1 cut(s)	498
HapII	CCGG	2 cut(s)	264, 979
HhaI	GCGC	2 cut(s)	678, 862
Hin1II	CATG	4 cut(s)	47, 361, 914, 947
Hin6I	GCGC	2 cut(s)	676, 860
HinP1I	GCGC	2 cut(s)	676, 860
HincII	GTYRAC	1 cut(s)	696
HindII	GTYRAC	1 cut(s)	696
HinfI	GANTC	6 cut(s)	124, 184, 406, 544, 747, 1063
HpaII	CCGG	2 cut(s)	264, 979
HphI	GGTGA	1 cut(s)	10
Hpy166II	GTNNAC	3 cut(s)	554, 696, 890
Hpy188I	TCNGA	3 cut(s)	96, 427, 781
Hpy188III	TCNNGA	9 cut(s)	38, 128, 164, 188, 237, 344, 473, 659, 878
Hpy8I	GTNNAC	3 cut(s)	554, 696, 890
HpyAV	CCTTC	3 cut(s)	344, 407, 712
HpyCH4III	ACNGT	2 cut(s)	551, 558
HpyCH4IV	ACGT	3 cut(s)	414, 764, 933
HpyCH4V	TGCA	6 cut(s)	4, 366, 554, 587, 633, 937
HpyF10VI	GCNNNNNNNGC	3 cut(s)	10, 81, 399
HpyF3I	CTNAG	1 cut(s)	71
HpySE526I	ACGT	3 cut(s)	414, 764, 933
Hsp92II	CATG	4 cut(s)	47, 361, 914, 947
HspAI	GCGC	2 cut(s)	676, 860
Ksp22I	TGATCA	1 cut(s)	523
Kzo9I	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
LguI	GCTCTTC	1 cut(s)	954
LmnI	GCTCC	3 cut(s)	251, 604, 782
Lsp1109I	GCAGC	2 cut(s)	16, 405
LweI	GCATC	1 cut(s)	642
MaeI	CTAG	2 cut(s)	464, 846
MaeII	ACGT	3 cut(s)	414, 764, 933
MalI	GATC	8 cut(s)	36, 270, 288, 315, 506, 525, 738, 915
MboI	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
MboII	GAAGA	4 cut(s)	164, 332, 934, 971
MhlI	GDGCHC	2 cut(s)	556, 609
MlsI	TGGCCA	1 cut(s)	498
MluCI	AATT	6 cut(s)	140, 476, 611, 753, 1058, 1076
MluNI	TGGCCA	1 cut(s)	498
MlyI	GAGTC	2 cut(s)	415, 538
Mox20I	TGGCCA	1 cut(s)	498
MscI	TGGCCA	1 cut(s)	498
MseI	TTAA	4 cut(s)	195, 684, 864, 1010
MslI	CAYNNNNRTG	1 cut(s)	647
Msp20I	TGGCCA	1 cut(s)	498
MspI	CCGG	2 cut(s)	264, 979
MspR9I	CCNGG	6 cut(s)	22, 500, 620, 819, 952, 1083
MvaI	CCWGG	6 cut(s)	22, 500, 620, 819, 952, 1083
MwoI	GCNNNNNNNGC	3 cut(s)	10, 81, 399
NdeI	CATATG	3 cut(s)	589, 742, 827
NdeII	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
NlaIII	CATG	4 cut(s)	47, 361, 914, 947
NlaIV	GGNNCC	2 cut(s)	778, 1025
NspI	RCATGY	1 cut(s)	47
NspV	TTCGAA	1 cut(s)	705
PaeR7I	CTCGAG	1 cut(s)	403
PciSI	GCTCTTC	1 cut(s)	954
PfeI	GAWTC	4 cut(s)	124, 184, 747, 1063
PkrI	GCNGC	2 cut(s)	6, 395
Ple19I	CGATCG	1 cut(s)	271
PleI	GAGTC	2 cut(s)	414, 538
PmaCI	CACGTG	1 cut(s)	765
PmlI	CACGTG	1 cut(s)	765
PpsI	GAGTC	2 cut(s)	414, 538
Ppu21I	YACGTR	2 cut(s)	765, 934
PsiI	TTATAA	1 cut(s)	303
Psp6I	CCWGG	6 cut(s)	20, 498, 618, 817, 950, 1081
PspCI	CACGTG	1 cut(s)	765
PspFI	CCCAGC	1 cut(s)	832
PspGI	CCWGG	6 cut(s)	20, 498, 618, 817, 950, 1081
PspN4I	GGNNCC	2 cut(s)	778, 1025
PspPI	GGNCC	2 cut(s)	981, 1024
PspXI	VCTCGAGB	1 cut(s)	403
PstNI	CAGNNNCTG	1 cut(s)	10
PvuI	CGATCG	1 cut(s)	271
RsaI	GTAC	4 cut(s)	30, 540, 891, 909
RsaNI	GTAC	4 cut(s)	29, 539, 890, 908
RseI	CAYNNNNRTG	1 cut(s)	647
SapI	GCTCTTC	1 cut(s)	954
SaqAI	TTAA	4 cut(s)	195, 684, 864, 1010
SatI	GCNGC	2 cut(s)	5, 394
Sau3AI	GATC	8 cut(s)	34, 268, 286, 313, 504, 523, 736, 913
Sau96I	GGNCC	2 cut(s)	981, 1024
SchI	GAGTC	2 cut(s)	415, 538
ScrFI	CCNGG	6 cut(s)	22, 500, 620, 819, 952, 1083
SduI	GDGCHC	2 cut(s)	556, 609
SfaNI	GCATC	1 cut(s)	642
Sfr274I	CTCGAG	1 cut(s)	403
SfuI	TTCGAA	1 cut(s)	705
SinI	GGWCC	2 cut(s)	981, 1024
SlaI	CTCGAG	1 cut(s)	403
SmiMI	CAYNNNNRTG	1 cut(s)	647
SmlI	CTYRAG	1 cut(s)	403
SmoI	CTYRAG	1 cut(s)	403
Sse9I	AATT	6 cut(s)	140, 476, 611, 753, 1058, 1076
SsiI	CCGC	2 cut(s)	111, 379
SspMI	CTAG	2 cut(s)	464, 846
StyD4I	CCNGG	6 cut(s)	20, 498, 618, 817, 950, 1081
StyI	CCWWGG	2 cut(s)	52, 308
TaaI	ACNGT	2 cut(s)	551, 558
TaiI	ACGT	3 cut(s)	417, 767, 936
TaqI	TCGA	3 cut(s)	187, 404, 705
TasI	AATT	6 cut(s)	140, 476, 611, 753, 1058, 1076
TatI	WGTACW	2 cut(s)	538, 889
TfiI	GAWTC	4 cut(s)	124, 184, 747, 1063
Tru1I	TTAA	4 cut(s)	195, 684, 864, 1010
Tru9I	TTAA	4 cut(s)	195, 684, 864, 1010
TscAI	CASTG	3 cut(s)	183, 561, 649
TseI	GCWGC	2 cut(s)	4, 393
TspDTI	ATGAA	5 cut(s)	550, 670, 816, 935, 960
TspRI	CASTG	3 cut(s)	183, 561, 649
VneI	GTGCAC	1 cut(s)	552
VpaK11BI	GGWCC	2 cut(s)	981, 1024
XapI	RAATTY	3 cut(s)	140, 611, 753
XceI	RCATGY	1 cut(s)	47
XcmI	CCANNNNNNNNNTGG	1 cut(s)	825
XhoI	CTCGAG	1 cut(s)	403
XspI	CTAG	2 cut(s)	464, 846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.