Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0004536

Physical Location & Seq

Genomic Coordinates Reverse (-)

1159180 .. 1160394

Gene Structure 1215 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG015000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1215 bp

ATGGCTTCTTGTTTTGATGAACGCGTACCGAGCTGGACAGTATCAAAAAGAGAAGAGGTCCTCAAATTAATAACCCTGAACAGCGCAATGTACACCAGAATGATGATGAATTATGAGGACGCTCCAATAGAAGATCGCAATCGTCACGAGGCCAAAGACCTCTCTATAGAGGAAGTACAGGAGAAACTTGAAGCTCTCAAGAAAAAATTAGATGATGGGAGCTTTAGTTGGATTTCACAAGACAAGTCCAACGTGACCTATGGTGAGTGCAGCGATGGCGTCAAACGTCTTACATTGAAGGATATAGATAAACGATCTAAACCAAGAGCTGAAAAAAGAGAAAAGATCCGTTCGATGGTTGATCTTATTCTCGGGTTCTTCCAAGCAAACAAGGAAACACATCGTGGGGTGTTTTACGTAGGGAATAATACCGCCAGTTCATTGTTCACACAAGACGAGGAGTACGCAATTCTTAATGACATCTGTTGCACTATAGGCTGCTGCAGTTCAAGTCTCTACATAGATGATGCTAGTGTAAGAGGCGTTGTTGGAGGTCTGCTTTCGTGGGTGCAGAATGGGTACGAAACCGATTGTCGAAGCCGGGATGATGGGGTGGCCATCCCGTCCATGCATTCAGATATTAGTTTGAAAAATTGTGGAGCCAAGTTCGTATTGGTGGTGGAAAACAAAGCCAGTTTTTATAATCTCATGCAAGACAGGTTTTACCGTGATTATCCATGCATAATCATCACCGGAATGGGAAAGCCCGGGGTGGCAACCAGAAGATTTTTAAAGCTATTGTCGGACAATTTTAGGCTTCCAGTGTATGGCCTTTTCGACTGTGATCCAGCAAGCATCGAAATGTTTTCGATTTATACTGTTGGGTCATATGAGGAGGCCTTCCATAGTGTAAACTTAACTTGCCCGTATATGACATGGTTGGGTGTTTGGCCGAGTGACCTATGTGCGCTTGACATACCCTGCGACGATATGAGTCCAAAAGAGATTAGAGACATGGATAATCTTTTGAAAGAAGATTTTGTGAAAGATAATCCGAGGTTGGTTGAGGAGCTGGAACATATGAAAGCGACGGAGAAAAAAGGGAATCTTGAAGCTTTGAAGGAGTTTGGGCCCACTTGTTTGTCCAAGTGCTATTTGCCATATAAGTTTAATTATATTTTGACCGCCGTTCACCGTGACAAGTGCACCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030957 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046983 GO:0048285 GO:0050896 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

404

Amino Acids

46.12

Weight (kDa)

5.32

Isoelectric Point (pI)

41.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP6A-Spo11_Toprim	PF21180	223 - 389	6.3e-47	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004536)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43731 FvH4_3g43753
rosa_chinensis	RchiOBHm_Chr2g0086091 RchiOBHm_Chr2g0089291 RchiOBHm_Chr2g0089851 RchiOBHm_Chr5g0083311
rosa_laevigata	RLG00000015741 RLG00000016061 RLG00000017209
rosa_multiflora	Rmu_co8293021.1_g000001 Rmu_co8360049.1_g000001 Rmu_sc0000388.1_g000047 Rmu_sc0002384.1_g000009 Rmu_sc0003382.1_g000021 Rmu_sc0017765.1_g000002 Rmu_ssc0000355.1_g000016
rosa_roxburghii	Rroxscaffold_2G00140180 Rroxscaffold_2G00151540
rosa_rugosa	Rorug01G0465600 Rorug01G0488800 Rorug02G0003700
rosa_samantha	Rh2AG015000 Rh2AG045100 Rh2AG048900 Rh2BG047800 Rh2CG016000 Rh2CG016100 Rh2CG045000 Rh2CG049900 Rh5BG561000 Rh5CG583600
rosa_wichuraiana	Rw2G001320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	702
AccB7I	CCANNNNNTGG	1 cut(s)	827
AccII	CGCG	1 cut(s)	24
AciI	CCGC	2 cut(s)	432, 1185
AclWI	GGATC	2 cut(s)	340, 839
AcoI	YGGCCR	2 cut(s)	615, 950
AcyI	GRCGYC	1 cut(s)	279
AdeI	CACNNNGTG	1 cut(s)	404
AfaI	GTAC	5 cut(s)	27, 92, 177, 464, 581
AfiI	CCNNNNNNNGG	2 cut(s)	355, 827
AflIII	ACRYGT	1 cut(s)	22
AgsI	TTSAA	7 cut(s)	191, 298, 510, 649, 1030, 1112, 1120
AluBI	AGCT	7 cut(s)	33, 194, 222, 329, 796, 1072, 1115
AluI	AGCT	7 cut(s)	33, 194, 222, 329, 796, 1072, 1115
Alw21I	GWGCWC	1 cut(s)	1208
Alw26I	GTCTC	2 cut(s)	518, 1005
Alw44I	GTGCAC	1 cut(s)	1204
AlwI	GGATC	2 cut(s)	340, 839
Ama87I	CYCGRG	2 cut(s)	371, 767
AoxI	GGCC	6 cut(s)	150, 615, 829, 897, 950, 1130
ApaI	GGGCCC	1 cut(s)	1134
ApaLI	GTGCAC	1 cut(s)	1204
ApeKI	GCWGC	3 cut(s)	270, 498, 501
AseI	ATTAAT	1 cut(s)	68
AspLEI	GCGC	2 cut(s)	86, 970
AspS9I	GGNCC	3 cut(s)	58, 1130, 1131
AsuC2I	CCSGG	3 cut(s)	602, 768, 769
AsuHPI	GGTGA	3 cut(s)	275, 742, 1184
AvaI	CYCGRG	2 cut(s)	371, 767
AvaII	GGWCC	1 cut(s)	58
BaeGI	GKGCMC	2 cut(s)	1134, 1208
BalI	TGGCCA	1 cut(s)	617
BanII	GRGCYC	1 cut(s)	1134
BauI	CACGAG	1 cut(s)	146
Bbv12I	GWGCWC	1 cut(s)	1208
BbvI	GCAGC	3 cut(s)	282, 485, 488
BccI	CCATC	5 cut(s)	209, 269, 349, 602, 626
BceAI	ACGGC	1 cut(s)	1172
BcnI	CCSGG	3 cut(s)	602, 768, 769
BcoDI	GTCTC	2 cut(s)	518, 1005
BfaI	CTAG	1 cut(s)	531
BfmI	CTRYAG	3 cut(s)	165, 492, 502
BisI	GCNGC	3 cut(s)	271, 499, 502
BlsI	GCNGC	3 cut(s)	272, 500, 503
Bme1390I	CCNGG	3 cut(s)	602, 768, 769
Bme18I	GGWCC	1 cut(s)	58
BmeT110I	CYCGRG	2 cut(s)	371, 767
BmgT120I	GGNCC	3 cut(s)	58, 1130, 1131
BmiI	GGNNCC	2 cut(s)	661, 1132
BmrFI	CCNGG	3 cut(s)	602, 768, 769
BmsI	GCATC	2 cut(s)	517, 864
BpuEI	CTTGAG	1 cut(s)	182
BpuMI	CCSGG	3 cut(s)	602, 768, 769
BsaAI	YACGTR	1 cut(s)	418
BsaBI	GATNNNNATC	1 cut(s)	138
BsaHI	GRCGYC	1 cut(s)	279
BsaJI	CCNNGG	3 cut(s)	767, 768, 1055
BsaWI	WCCGGW	1 cut(s)	752
BsaXI	ACNNNNNCTCC	2 cut(s)	211, 241
Bsc4I	CCNNNNNNNGG	2 cut(s)	355, 827
Bse1I	ACTGG	3 cut(s)	435, 693, 821
Bse3DI	GCAATG	1 cut(s)	93
Bse8I	GATNNNNATC	1 cut(s)	138
BseDI	CCNNGG	3 cut(s)	767, 768, 1055
BseGI	GGATG	2 cut(s)	610, 618
BseJI	GATNNNNATC	1 cut(s)	138
BseLI	CCNNNNNNNGG	2 cut(s)	355, 827
BseMI	GCAATG	1 cut(s)	93
BseNI	ACTGG	3 cut(s)	435, 693, 821
BseRI	GAGGAG	3 cut(s)	473, 908, 1082
BseSI	GKGCMC	2 cut(s)	1134, 1208
BseXI	GCAGC	3 cut(s)	282, 485, 488
BsgI	GTGCAG	2 cut(s)	289, 590
Bsh1236I	CGCG	1 cut(s)	24
BshFI	GGCC	6 cut(s)	152, 617, 831, 899, 952, 1132
BsiHKAI	GWGCWC	1 cut(s)	1208
BsiHKCI	CYCGRG	2 cut(s)	371, 767
BsiSI	CCGG	3 cut(s)	601, 753, 768
BslI	CCNNNNNNNGG	2 cut(s)	355, 827
BsmAI	GTCTC	2 cut(s)	518, 1005
BsmI	GAATGC	1 cut(s)	631
BsnI	GGCC	6 cut(s)	152, 617, 831, 899, 952, 1132
BsoBI	CYCGRG	2 cut(s)	371, 767
Bsp120I	GGGCCC	1 cut(s)	1130
Bsp1286I	GDGCHC	2 cut(s)	1134, 1208
Bsp1407I	TGTACA	1 cut(s)	90
Bsp143I	GATC	5 cut(s)	133, 314, 345, 361, 844
BspACI	CCGC	2 cut(s)	432, 1185
BspANI	GGCC	6 cut(s)	152, 617, 831, 899, 952, 1132
BspFNI	CGCG	1 cut(s)	24
BspLI	GGNNCC	2 cut(s)	661, 1132
BspMAI	CTGCAG	1 cut(s)	506
BspPI	GGATC	2 cut(s)	340, 839
BsrDI	GCAATG	1 cut(s)	93
BsrGI	TGTACA	1 cut(s)	90
BsrI	ACTGG	3 cut(s)	435, 693, 821
BssECI	CCNNGG	3 cut(s)	767, 768, 1055
BssMI	GATC	5 cut(s)	133, 314, 345, 361, 844
BssNI	GRCGYC	1 cut(s)	279
BssSI	CACGAG	1 cut(s)	146
Bst2BI	CACGAG	1 cut(s)	146
Bst4CI	ACNGT	5 cut(s)	40, 728, 842, 880, 1196
Bst6I	CTCTTC	1 cut(s)	48
BstACI	GRCGYC	1 cut(s)	279
BstAUI	TGTACA	1 cut(s)	90
BstBAI	YACGTR	1 cut(s)	418
BstC8I	GCNNGC	1 cut(s)	853
BstF5I	GGATG	2 cut(s)	610, 618
BstFNI	CGCG	1 cut(s)	24
BstHHI	GCGC	2 cut(s)	86, 970
BstKTI	GATC	5 cut(s)	136, 317, 348, 364, 847
BstMAI	GTCTC	2 cut(s)	518, 1005
BstMBI	GATC	5 cut(s)	133, 314, 345, 361, 844
BstMWI	GCNNNNNNNGC	3 cut(s)	30, 276, 495
BstSCI	CCNGG	3 cut(s)	600, 766, 767
BstSFI	CTRYAG	3 cut(s)	165, 492, 502
BstSLI	GKGCMC	2 cut(s)	1134, 1208
BstSNI	TACGTA	1 cut(s)	418
BstUI	CGCG	1 cut(s)	24
BstV1I	GCAGC	3 cut(s)	282, 485, 488
BstX2I	RGATCY	1 cut(s)	345
BstYI	RGATCY	1 cut(s)	345
BsuRI	GGCC	6 cut(s)	152, 617, 831, 899, 952, 1132
BtgZI	GCGATG	1 cut(s)	288
BtsCI	GGATG	2 cut(s)	610, 618
BtsIMutI	CAGTG	1 cut(s)	828
Cac8I	GCNNGC	1 cut(s)	853
CfoI	GCGC	2 cut(s)	86, 970
Cfr13I	GGNCC	3 cut(s)	58, 1130, 1131
Cfr9I	CCCGGG	1 cut(s)	767
CseI	GACGC	2 cut(s)	128, 268
Csp6I	GTAC	5 cut(s)	26, 91, 176, 463, 580
CviAII	CATG	5 cut(s)	628, 709, 738, 936, 1015
CviQI	GTAC	5 cut(s)	26, 91, 176, 463, 580
DpnI	GATC	5 cut(s)	135, 316, 347, 363, 846
DpnII	GATC	5 cut(s)	133, 314, 345, 361, 844
DraI	TTTAAA	1 cut(s)	792
DraIII	CACNNNGTG	1 cut(s)	404
EaeI	YGGCCR	2 cut(s)	615, 950
Eam1104I	CTCTTC	1 cut(s)	48
EarI	CTCTTC	1 cut(s)	48
Eco105I	TACGTA	1 cut(s)	418
Eco147I	AGGCCT	1 cut(s)	899
Eco24I	GRGCYC	1 cut(s)	1134
Eco47I	GGWCC	1 cut(s)	58
Eco88I	CYCGRG	2 cut(s)	371, 767
EcoO109I	RGGNCCY	1 cut(s)	58
EcoT22I	ATGCAT	2 cut(s)	633, 743
EcoT38I	GRGCYC	1 cut(s)	1134
FaeI	CATG	5 cut(s)	631, 712, 741, 939, 1018
FalI	AAGNNNNNCTT	2 cut(s)	1092, 1124
FatI	CATG	5 cut(s)	627, 708, 737, 935, 1014
FauNDI	CATATG	2 cut(s)	889, 1080
Fnu4HI	GCNGC	3 cut(s)	271, 499, 502
FokI	GGATG	2 cut(s)	605, 617
FriOI	GRGCYC	1 cut(s)	1134
Fsp4HI	GCNGC	3 cut(s)	271, 499, 502
FspBI	CTAG	1 cut(s)	531
GlaI	GCGC	2 cut(s)	85, 969
GluI	GCNGC	3 cut(s)	271, 499, 502
HaeIII	GGCC	6 cut(s)	152, 617, 831, 899, 952, 1132
HapII	CCGG	3 cut(s)	601, 753, 768
HgaI	GACGC	2 cut(s)	128, 268
HhaI	GCGC	2 cut(s)	86, 970
Hin1I	GRCGYC	1 cut(s)	279
Hin1II	CATG	5 cut(s)	631, 712, 741, 939, 1018
Hin6I	GCGC	2 cut(s)	84, 968
HinP1I	GCGC	2 cut(s)	84, 968
HindIII	AAGCTT	1 cut(s)	1113
HinfI	GANTC	2 cut(s)	994, 1105
HpaII	CCGG	3 cut(s)	601, 753, 768
HphI	GGTGA	3 cut(s)	275, 742, 1184
Hpy166II	GTNNAC	5 cut(s)	93, 447, 913, 1192, 1206
Hpy188I	TCNGA	3 cut(s)	637, 805, 1056
Hpy188III	TCNNGA	3 cut(s)	146, 199, 1109
Hpy8I	GTNNAC	5 cut(s)	93, 447, 913, 1192, 1206
Hpy99I	CGWCG	2 cut(s)	989, 1093
HpyAV	CCTTC	3 cut(s)	292, 910, 1114
HpyCH4III	ACNGT	5 cut(s)	40, 728, 842, 880, 1196
HpyCH4IV	ACGT	3 cut(s)	252, 286, 417
HpyCH4V	TGCA	8 cut(s)	270, 489, 504, 571, 631, 712, 741, 1206
HpyF10VI	GCNNNNNNNGC	3 cut(s)	30, 276, 495
HpySE526I	ACGT	3 cut(s)	252, 286, 417
Hsp92I	GRCGYC	1 cut(s)	279
Hsp92II	CATG	5 cut(s)	631, 712, 741, 939, 1018
HspAI	GCGC	2 cut(s)	84, 968
Kzo9I	GATC	5 cut(s)	133, 314, 345, 361, 844
LmnI	GCTCC	4 cut(s)	127, 219, 659, 1069
Lsp1109I	GCAGC	3 cut(s)	282, 485, 488
LweI	GCATC	2 cut(s)	517, 864
MaeI	CTAG	1 cut(s)	531
MaeII	ACGT	3 cut(s)	252, 286, 417
MaeIII	GTNAC	4 cut(s)	143, 253, 956, 1196
MalI	GATC	5 cut(s)	135, 316, 347, 363, 846
MboI	GATC	5 cut(s)	133, 314, 345, 361, 844
MboII	GAAGA	5 cut(s)	65, 143, 370, 795, 1046
MflI	RGATCY	1 cut(s)	345
MhlI	GDGCHC	2 cut(s)	1134, 1208
MlsI	TGGCCA	1 cut(s)	617
MluCI	AATT	8 cut(s)	65, 109, 206, 468, 652, 808, 1171, 1210
MluI	ACGCGT	1 cut(s)	22
MluNI	TGGCCA	1 cut(s)	617
MlyI	GAGTC	1 cut(s)	1003
MmeI	TCCRAC	4 cut(s)	209, 273, 529, 783
Mox20I	TGGCCA	1 cut(s)	617
Mph1103I	ATGCAT	2 cut(s)	633, 743
MscI	TGGCCA	1 cut(s)	617
MseI	TTAA	6 cut(s)	68, 474, 791, 917, 1170, 1213
MslI	CAYNNNNRTG	3 cut(s)	98, 755, 860
Msp20I	TGGCCA	1 cut(s)	617
MspI	CCGG	3 cut(s)	601, 753, 768
MspR9I	CCNGG	3 cut(s)	602, 768, 769
Mva1269I	GAATGC	1 cut(s)	631
MvnI	CGCG	1 cut(s)	24
MwoI	GCNNNNNNNGC	3 cut(s)	30, 276, 495
NciI	CCSGG	3 cut(s)	602, 768, 769
NdeI	CATATG	2 cut(s)	889, 1080
NdeII	GATC	5 cut(s)	133, 314, 345, 361, 844
NlaIII	CATG	5 cut(s)	631, 712, 741, 939, 1018
NlaIV	GGNNCC	2 cut(s)	661, 1132
NmeAIII	GCCGAG	1 cut(s)	978
NmuCI	GTSAC	4 cut(s)	143, 253, 956, 1196
NsiI	ATGCAT	2 cut(s)	633, 743
PceI	AGGCCT	1 cut(s)	899
PctI	GAATGC	1 cut(s)	631
PfeI	GAWTC	1 cut(s)	1105
PflMI	CCANNNNNTGG	1 cut(s)	827
PkrI	GCNGC	3 cut(s)	272, 500, 503
PleI	GAGTC	1 cut(s)	1002
PpsI	GAGTC	1 cut(s)	1002
Ppu21I	YACGTR	1 cut(s)	418
PpuMI	RGGWCCY	1 cut(s)	58
PshBI	ATTAAT	1 cut(s)	68
PsiI	TTATAA	1 cut(s)	702
Psp5II	RGGWCCY	1 cut(s)	58
PspN4I	GGNNCC	2 cut(s)	661, 1132
PspOMI	GGGCCC	1 cut(s)	1130
PspPI	GGNCC	3 cut(s)	58, 1130, 1131
PspPPI	RGGWCCY	1 cut(s)	58
PstI	CTGCAG	1 cut(s)	506
PsuI	RGATCY	1 cut(s)	345
RsaI	GTAC	5 cut(s)	27, 92, 177, 464, 581
RsaNI	GTAC	5 cut(s)	26, 91, 176, 463, 580
RseI	CAYNNNNRTG	3 cut(s)	98, 755, 860
SaqAI	TTAA	6 cut(s)	68, 474, 791, 917, 1170, 1213
SatI	GCNGC	3 cut(s)	271, 499, 502
Sau3AI	GATC	5 cut(s)	133, 314, 345, 361, 844
Sau96I	GGNCC	3 cut(s)	58, 1130, 1131
SchI	GAGTC	1 cut(s)	1003
ScrFI	CCNGG	3 cut(s)	602, 768, 769
SduI	GDGCHC	2 cut(s)	1134, 1208
SfaNI	GCATC	2 cut(s)	517, 864
SfcI	CTRYAG	3 cut(s)	165, 492, 502
SinI	GGWCC	1 cut(s)	58
SmaI	CCCGGG	1 cut(s)	769
SmiMI	CAYNNNNRTG	3 cut(s)	98, 755, 860
SmlI	CTYRAG	1 cut(s)	197
SmoI	CTYRAG	1 cut(s)	197
SnaBI	TACGTA	1 cut(s)	418
Sse9I	AATT	8 cut(s)	65, 109, 206, 468, 652, 808, 1171, 1210
SseBI	AGGCCT	1 cut(s)	899
SsiI	CCGC	2 cut(s)	432, 1185
SspMI	CTAG	1 cut(s)	531
StuI	AGGCCT	1 cut(s)	899
StyD4I	CCNGG	3 cut(s)	600, 766, 767
TaaI	ACNGT	5 cut(s)	40, 728, 842, 880, 1196
TaiI	ACGT	3 cut(s)	255, 289, 420
TaqI	TCGA	5 cut(s)	353, 595, 837, 858, 869
TasI	AATT	8 cut(s)	65, 109, 206, 468, 652, 808, 1171, 1210
TatI	WGTACW	2 cut(s)	90, 175
TfiI	GAWTC	1 cut(s)	1105
Tru1I	TTAA	6 cut(s)	68, 474, 791, 917, 1170, 1213
Tru9I	TTAA	6 cut(s)	68, 474, 791, 917, 1170, 1213
TscAI	CASTG	1 cut(s)	828
TseFI	GTSAC	4 cut(s)	143, 253, 956, 1196
TseI	GCWGC	3 cut(s)	270, 498, 501
Tsp45I	GTSAC	4 cut(s)	143, 253, 956, 1196
TspDTI	ATGAA	4 cut(s)	33, 122, 429, 1097
TspGWI	ACGGA	2 cut(s)	338, 1106
TspMI	CCCGGG	1 cut(s)	767
TspRI	CASTG	1 cut(s)	828
Van91I	CCANNNNNTGG	1 cut(s)	827
VneI	GTGCAC	1 cut(s)	1204
VpaK11BI	GGWCC	1 cut(s)	58
VspI	ATTAAT	1 cut(s)	68
XcmI	CCANNNNNNNNNTGG	1 cut(s)	670
XmaI	CCCGGG	1 cut(s)	767
XspI	CTAG	1 cut(s)	531
Zsp2I	ATGCAT	2 cut(s)	633, 743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.