Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0004536

Physical Location & Seq

Genomic Coordinates Reverse (-)

3393578 .. 3394857

Gene Structure 1280 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG047800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1185 bp

ATGGCTTCTTGTTTTGATGAACGCAAACCAATCTGGCCAATACCAAAAAGAGAAGAGGTCCGCCTATTAATGAACCTACACAGCGCAATGTACACCAGACTGATGGCTTATGAGAACGCTCTAATAGAAGATCCCAATCGTTACGAGGTCAAAGACCTCTCTATAGAGGAAGTACAGGAGAAACTTGAAGCTCTCAAGAAAAAATTAGATGATGGGAGCTTTAGTTGCATTTCACGAGACAAGTCCAACGTGACCTATGGTGAGTGTAGCGATGGCGTCAAACGTCTTACAGTGAAGGATATAAATAAACGCTCTAAACCAGGAGATGAAAAAAGAAAAAATATCCGTTTGATGGTTGATCTTATTCTCAAGTTCTTCCAAGCAAACAAGCGAGAAACACATCGTGGGGTGTTTTACGTAGGGAATAATACCAGTTCATTGTTCCGACAAAAAGATGAGTACGAAATTCTTGATGACATCTGTTGTACTATAAGGTGCAGCAGTTCAAGTCTCTACGTAGATGCTAGTGTAAGAGGCGTTGTTGCAGGTCTGCTTTCTTTCGAGCTTGGAGGGCATGAAACCTTTTGTACGAGCCGGGACGATGGGGTACCCATCCCGCTGAATTCAGATATTAAAATGAAGAATCGTGGAGCCAAGTTCATATTGGTGGTGGAAAATAAATCCGCTTTCAATACATTGATGCAAGATAAGTTTTACAATGATTATCCATGTATAATCATCACCGGAATGGGAATGCCGGAGGTGGCGACCAGAAGATTTTTAAAGCTATTGTCGGACAATTTTGGGCTTCCAGTGTATGGCCTTTTCGACTGTGATCCAGAAGGCATCAAAATGTTTACAATTTATACTGTTGGATCATATGAGGATGCCTTCGATAGTGTAAACTTAACTTGGCCGTATATGACGTGGTTGGGTGTTTGGCCAAGTGACCTATGTGCGCTTGATATACCCTGCAAGGATTTGAGTCCGAGTGAGATTGCATTGGTAGATTATCTTTTGAGAGAAGATTTTGTGAAAGAGAATCCGAGGTTGGTTGAGGAGCTGGAACATATGAAAATAACGGAGAAAAAAGCGAATCTTGAAGCTTTGAAGGAATTTGGGCCCACTTGTTTGTCTAAGTACTATTTGCCATATAAGTTGAATTATCTTTTGACCGCGCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030957 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046983 GO:0048285 GO:0050896 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

394

Amino Acids

45.2

Weight (kDa)

5.55

Isoelectric Point (pI)

42.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP6A-Spo11_Toprim	PF21180	220 - 387	1.7e-47	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004536)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43731 FvH4_3g43753
rosa_chinensis	RchiOBHm_Chr2g0086091 RchiOBHm_Chr2g0089291 RchiOBHm_Chr2g0089851 RchiOBHm_Chr5g0083311
rosa_laevigata	RLG00000015741 RLG00000016061 RLG00000017209
rosa_multiflora	Rmu_co8293021.1_g000001 Rmu_co8360049.1_g000001 Rmu_sc0000388.1_g000047 Rmu_sc0002384.1_g000009 Rmu_sc0003382.1_g000021 Rmu_sc0017765.1_g000002 Rmu_ssc0000355.1_g000016
rosa_roxburghii	Rroxscaffold_2G00140180 Rroxscaffold_2G00151540
rosa_rugosa	Rorug01G0465600 Rorug01G0488800 Rorug02G0003700
rosa_samantha	Rh2AG015000 Rh2AG045100 Rh2AG048900 Rh2BG047800 Rh2CG016000 Rh2CG016100 Rh2CG045000 Rh2CG049900 Rh5BG561000 Rh5CG583600
rosa_wichuraiana	Rw2G001320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	536
Acc65I	GGTACC	1 cut(s)	607
AccB1I	GGYRCC	1 cut(s)	607
AccB7I	CCANNNNNTGG	1 cut(s)	818
AccII	CGCG	1 cut(s)	1178
AciI	CCGC	4 cut(s)	61, 617, 684, 1176
AclWI	GGATC	3 cut(s)	125, 830, 883
AcoI	YGGCCR	3 cut(s)	35, 914, 941
AcsI	RAATTY	3 cut(s)	465, 622, 1115
AcyI	GRCGYC	1 cut(s)	276
AdeI	CACNNNGTG	1 cut(s)	404
AfaI	GTAC	7 cut(s)	92, 174, 461, 487, 589, 609, 1142
AfiI	CCNNNNNNNGG	2 cut(s)	352, 818
AgsI	TTSAA	6 cut(s)	188, 507, 691, 1103, 1111, 1162
AjiI	CACGTC	1 cut(s)	927
AjnI	CCWGG	1 cut(s)	319
AjuI	GAANNNNNNNTTGG	2 cut(s)	1034, 1066
AluBI	AGCT	6 cut(s)	191, 219, 565, 787, 1063, 1106
AluI	AGCT	6 cut(s)	191, 219, 565, 787, 1063, 1106
Alw26I	GTCTC	2 cut(s)	231, 515
AlwI	GGATC	3 cut(s)	125, 830, 883
AoxI	GGCC	5 cut(s)	35, 820, 914, 941, 1121
ApaI	GGGCCC	1 cut(s)	1125
ApeKI	GCWGC	1 cut(s)	498
ApoI	RAATTY	3 cut(s)	465, 622, 1115
AseI	ATTAAT	1 cut(s)	68
Asp718I	GGTACC	1 cut(s)	607
AspLEI	GCGC	3 cut(s)	86, 961, 1180
AspS9I	GGNCC	3 cut(s)	58, 1121, 1122
AsuC2I	CCSGG	1 cut(s)	596
AsuHPI	GGTGA	2 cut(s)	272, 733
AvaII	GGWCC	1 cut(s)	58
BaeGI	GKGCMC	1 cut(s)	1125
BalI	TGGCCA	2 cut(s)	37, 943
BanI	GGYRCC	1 cut(s)	607
BanII	GRGCYC	1 cut(s)	1125
BauI	CACGAG	1 cut(s)	234
BbvI	GCAGC	1 cut(s)	510
BccI	CCATC	6 cut(s)	97, 206, 266, 346, 596, 620
BceAI	ACGGC	2 cut(s)	901, 1165
BciT130I	CCWGG	1 cut(s)	321
BcnI	CCSGG	1 cut(s)	596
BcoDI	GTCTC	2 cut(s)	231, 515
BfaI	CTAG	1 cut(s)	525
BfmI	CTRYAG	1 cut(s)	162
BfuAI	ACCTGC	1 cut(s)	536
BisI	GCNGC	1 cut(s)	499
BlsI	GCNGC	1 cut(s)	500
BmcAI	AGTACT	1 cut(s)	1142
Bme1390I	CCNGG	2 cut(s)	321, 596
Bme18I	GGWCC	1 cut(s)	58
BmgBI	CACGTC	1 cut(s)	927
BmgT120I	GGNCC	3 cut(s)	58, 1121, 1122
BmiI	GGNNCC	3 cut(s)	609, 652, 1123
BmrFI	CCNGG	2 cut(s)	321, 596
BmsI	GCATC	4 cut(s)	511, 690, 855, 877
BpuEI	CTTGAG	2 cut(s)	179, 353
BpuMI	CCSGG	1 cut(s)	596
BsaAI	YACGTR	2 cut(s)	418, 517
BsaBI	GATNNNNATC	1 cut(s)	135
BsaHI	GRCGYC	1 cut(s)	276
BsaJI	CCNNGG	1 cut(s)	1046
BsaWI	WCCGGW	1 cut(s)	743
BsaXI	ACNNNNNCTCC	2 cut(s)	208, 238
Bsc4I	CCNNNNNNNGG	2 cut(s)	352, 818
Bse1I	ACTGG	2 cut(s)	432, 812
Bse3DI	GCAATG	1 cut(s)	93
Bse8I	GATNNNNATC	1 cut(s)	135
BseBI	CCWGG	1 cut(s)	321
BseDI	CCNNGG	1 cut(s)	1046
BseGI	GGATG	2 cut(s)	612, 892
BseJI	GATNNNNATC	1 cut(s)	135
BseLI	CCNNNNNNNGG	2 cut(s)	352, 818
BseMI	GCAATG	1 cut(s)	93
BseNI	ACTGG	2 cut(s)	432, 812
BseRI	GAGGAG	1 cut(s)	1073
BseSI	GKGCMC	1 cut(s)	1125
BseXI	GCAGC	1 cut(s)	510
BsgI	GTGCAG	1 cut(s)	517
Bsh1236I	CGCG	1 cut(s)	1178
BshFI	GGCC	5 cut(s)	37, 822, 916, 943, 1123
BshNI	GGYRCC	1 cut(s)	607
BsiSI	CCGG	3 cut(s)	595, 744, 758
BslFI	GGGAC	1 cut(s)	611
BslI	CCNNNNNNNGG	2 cut(s)	352, 818
BsmAI	GTCTC	2 cut(s)	231, 515
BsmFI	GGGAC	1 cut(s)	611
BsmI	GAATGC	1 cut(s)	759
BsnI	GGCC	5 cut(s)	37, 822, 916, 943, 1123
Bsp120I	GGGCCC	1 cut(s)	1121
Bsp1286I	GDGCHC	1 cut(s)	1125
Bsp1407I	TGTACA	1 cut(s)	90
Bsp143I	GATC	4 cut(s)	130, 358, 835, 875
BspACI	CCGC	4 cut(s)	61, 617, 684, 1176
BspANI	GGCC	5 cut(s)	37, 822, 916, 943, 1123
BspFNI	CGCG	1 cut(s)	1178
BspLI	GGNNCC	3 cut(s)	609, 652, 1123
BspMI	ACCTGC	1 cut(s)	536
BspPI	GGATC	3 cut(s)	125, 830, 883
BspT107I	GGYRCC	1 cut(s)	607
BsrDI	GCAATG	1 cut(s)	93
BsrGI	TGTACA	1 cut(s)	90
BsrI	ACTGG	2 cut(s)	432, 812
BssECI	CCNNGG	1 cut(s)	1046
BssMI	GATC	4 cut(s)	130, 358, 835, 875
BssNI	GRCGYC	1 cut(s)	276
BssSI	CACGAG	1 cut(s)	234
Bst2BI	CACGAG	1 cut(s)	234
Bst2UI	CCWGG	1 cut(s)	321
Bst4CI	ACNGT	3 cut(s)	292, 833, 871
Bst6I	CTCTTC	1 cut(s)	48
BstACI	GRCGYC	1 cut(s)	276
BstAUI	TGTACA	1 cut(s)	90
BstBAI	YACGTR	2 cut(s)	418, 517
BstDEI	CTNAG	1 cut(s)	1137
BstF5I	GGATG	2 cut(s)	612, 892
BstFNI	CGCG	1 cut(s)	1178
BstHHI	GCGC	3 cut(s)	86, 961, 1180
BstKTI	GATC	4 cut(s)	133, 361, 838, 878
BstMAI	GTCTC	2 cut(s)	231, 515
BstMBI	GATC	4 cut(s)	130, 358, 835, 875
BstMWI	GCNNNNNNNGC	2 cut(s)	225, 571
BstNI	CCWGG	1 cut(s)	321
BstSCI	CCNGG	2 cut(s)	319, 594
BstSFI	CTRYAG	1 cut(s)	162
BstSLI	GKGCMC	1 cut(s)	1125
BstSNI	TACGTA	2 cut(s)	418, 517
BstUI	CGCG	1 cut(s)	1178
BstV1I	GCAGC	1 cut(s)	510
BstX2I	RGATCY	1 cut(s)	130
BstXI	CCANNNNNNTGG	1 cut(s)	103
BstYI	RGATCY	1 cut(s)	130
BsuRI	GGCC	5 cut(s)	37, 822, 916, 943, 1123
BtgZI	GCGATG	1 cut(s)	285
BtrI	CACGTC	1 cut(s)	927
BtsCI	GGATG	2 cut(s)	612, 892
BtsIMutI	CAGTG	2 cut(s)	297, 819
BveI	ACCTGC	1 cut(s)	536
CfoI	GCGC	3 cut(s)	86, 961, 1180
Cfr13I	GGNCC	3 cut(s)	58, 1121, 1122
CseI	GACGC	1 cut(s)	265
Csp6I	GTAC	7 cut(s)	91, 173, 460, 486, 588, 608, 1141
CviAII	CATG	2 cut(s)	575, 729
CviQI	GTAC	7 cut(s)	91, 173, 460, 486, 588, 608, 1141
DdeI	CTNAG	1 cut(s)	1137
DpnI	GATC	4 cut(s)	132, 360, 837, 877
DpnII	GATC	4 cut(s)	130, 358, 835, 875
DraI	TTTAAA	1 cut(s)	783
DraIII	CACNNNGTG	1 cut(s)	404
EaeI	YGGCCR	3 cut(s)	35, 914, 941
Eam1104I	CTCTTC	1 cut(s)	48
EarI	CTCTTC	1 cut(s)	48
EciI	GGCGGA	1 cut(s)	50
Eco105I	TACGTA	2 cut(s)	418, 517
Eco24I	GRGCYC	1 cut(s)	1125
Eco47I	GGWCC	1 cut(s)	58
EcoRI	GAATTC	1 cut(s)	622
EcoRII	CCWGG	1 cut(s)	319
EcoT38I	GRGCYC	1 cut(s)	1125
FaeI	CATG	2 cut(s)	578, 732
FalI	AAGNNNNNCTT	2 cut(s)	1083, 1115
FaqI	GGGAC	1 cut(s)	611
FatI	CATG	2 cut(s)	574, 728
FauI	CCCGC	1 cut(s)	624
FauNDI	CATATG	2 cut(s)	880, 1071
Fnu4HI	GCNGC	1 cut(s)	499
FokI	GGATG	2 cut(s)	599, 899
FriOI	GRGCYC	1 cut(s)	1125
Fsp4HI	GCNGC	1 cut(s)	499
FspBI	CTAG	1 cut(s)	525
GlaI	GCGC	3 cut(s)	85, 960, 1179
GluI	GCNGC	1 cut(s)	499
HaeIII	GGCC	5 cut(s)	37, 822, 916, 943, 1123
HapII	CCGG	3 cut(s)	595, 744, 758
HgaI	GACGC	1 cut(s)	265
HhaI	GCGC	3 cut(s)	86, 961, 1180
Hin1I	GRCGYC	1 cut(s)	276
Hin1II	CATG	2 cut(s)	578, 732
Hin6I	GCGC	3 cut(s)	84, 959, 1178
HinP1I	GCGC	3 cut(s)	84, 959, 1178
HindIII	AAGCTT	1 cut(s)	1104
HinfI	GANTC	4 cut(s)	643, 985, 1042, 1096
HpaII	CCGG	3 cut(s)	595, 744, 758
HphI	GGTGA	2 cut(s)	272, 733
Hpy166II	GTNNAC	3 cut(s)	93, 858, 904
Hpy188I	TCNGA	5 cut(s)	446, 628, 796, 990, 1047
Hpy188III	TCNNGA	5 cut(s)	196, 234, 470, 839, 1100
Hpy8I	GTNNAC	3 cut(s)	93, 858, 904
HpyAV	CCTTC	4 cut(s)	289, 836, 901, 1105
HpyCH4III	ACNGT	3 cut(s)	292, 833, 871
HpyCH4IV	ACGT	5 cut(s)	249, 283, 417, 516, 926
HpyCH4V	TGCA	6 cut(s)	228, 498, 545, 703, 975, 1001
HpyF10VI	GCNNNNNNNGC	2 cut(s)	225, 571
HpyF3I	CTNAG	1 cut(s)	1137
HpySE526I	ACGT	5 cut(s)	249, 283, 417, 516, 926
Hsp92I	GRCGYC	1 cut(s)	276
Hsp92II	CATG	2 cut(s)	578, 732
HspAI	GCGC	3 cut(s)	84, 959, 1178
KpnI	GGTACC	1 cut(s)	611
Kzo9I	GATC	4 cut(s)	130, 358, 835, 875
LmnI	GCTCC	3 cut(s)	216, 650, 1060
Lsp1109I	GCAGC	1 cut(s)	510
LweI	GCATC	4 cut(s)	511, 690, 855, 877
MaeI	CTAG	1 cut(s)	525
MaeII	ACGT	5 cut(s)	249, 283, 417, 516, 926
MaeIII	GTNAC	3 cut(s)	140, 250, 947
MalI	GATC	4 cut(s)	132, 360, 837, 877
MboI	GATC	4 cut(s)	130, 358, 835, 875
MboII	GAAGA	6 cut(s)	65, 140, 367, 652, 786, 1037
MflI	RGATCY	1 cut(s)	130
MhlI	GDGCHC	1 cut(s)	1125
MlsI	TGGCCA	2 cut(s)	37, 943
MluCI	AATT	7 cut(s)	203, 465, 622, 799, 861, 1115, 1162
MluNI	TGGCCA	2 cut(s)	37, 943
MlyI	GAGTC	1 cut(s)	994
MmeI	TCCRAC	4 cut(s)	270, 469, 774, 853
Mox20I	TGGCCA	2 cut(s)	37, 943
MscI	TGGCCA	2 cut(s)	37, 943
MseI	TTAA	4 cut(s)	68, 633, 782, 908
MslI	CAYNNNNRTG	3 cut(s)	665, 746, 851
Msp20I	TGGCCA	2 cut(s)	37, 943
MspA1I	CMGCKG	1 cut(s)	619
MspI	CCGG	3 cut(s)	595, 744, 758
MspR9I	CCNGG	2 cut(s)	321, 596
Mva1269I	GAATGC	1 cut(s)	759
MvaI	CCWGG	1 cut(s)	321
MvnI	CGCG	1 cut(s)	1178
MwoI	GCNNNNNNNGC	2 cut(s)	225, 571
NciI	CCSGG	1 cut(s)	596
NdeI	CATATG	2 cut(s)	880, 1071
NdeII	GATC	4 cut(s)	130, 358, 835, 875
NlaIII	CATG	2 cut(s)	578, 732
NlaIV	GGNNCC	3 cut(s)	609, 652, 1123
NmuCI	GTSAC	2 cut(s)	250, 947
PctI	GAATGC	1 cut(s)	759
PfeI	GAWTC	3 cut(s)	643, 1042, 1096
PflMI	CCANNNNNTGG	1 cut(s)	818
PkrI	GCNGC	1 cut(s)	500
PleI	GAGTC	1 cut(s)	993
PpsI	GAGTC	1 cut(s)	993
Ppu21I	YACGTR	2 cut(s)	418, 517
PshBI	ATTAAT	1 cut(s)	68
Psp6I	CCWGG	1 cut(s)	319
PspGI	CCWGG	1 cut(s)	319
PspN4I	GGNNCC	3 cut(s)	609, 652, 1123
PspOMI	GGGCCC	1 cut(s)	1121
PspPI	GGNCC	3 cut(s)	58, 1121, 1122
PsuI	RGATCY	1 cut(s)	130
RsaI	GTAC	7 cut(s)	92, 174, 461, 487, 589, 609, 1142
RsaNI	GTAC	7 cut(s)	91, 173, 460, 486, 588, 608, 1141
RseI	CAYNNNNRTG	3 cut(s)	665, 746, 851
SaqAI	TTAA	4 cut(s)	68, 633, 782, 908
SatI	GCNGC	1 cut(s)	499
Sau3AI	GATC	4 cut(s)	130, 358, 835, 875
Sau96I	GGNCC	3 cut(s)	58, 1121, 1122
ScaI	AGTACT	1 cut(s)	1142
SchI	GAGTC	1 cut(s)	994
ScrFI	CCNGG	2 cut(s)	321, 596
SduI	GDGCHC	1 cut(s)	1125
SfaNI	GCATC	4 cut(s)	511, 690, 855, 877
SfcI	CTRYAG	1 cut(s)	162
SinI	GGWCC	1 cut(s)	58
SmiMI	CAYNNNNRTG	3 cut(s)	665, 746, 851
SmlI	CTYRAG	2 cut(s)	194, 368
SmoI	CTYRAG	2 cut(s)	194, 368
SnaBI	TACGTA	2 cut(s)	418, 517
Sse9I	AATT	7 cut(s)	203, 465, 622, 799, 861, 1115, 1162
SsiI	CCGC	4 cut(s)	61, 617, 684, 1176
SspMI	CTAG	1 cut(s)	525
StyD4I	CCNGG	2 cut(s)	319, 594
TaaI	ACNGT	3 cut(s)	292, 833, 871
TaiI	ACGT	5 cut(s)	252, 286, 420, 519, 929
TaqI	TCGA	3 cut(s)	561, 828, 894
TasI	AATT	7 cut(s)	203, 465, 622, 799, 861, 1115, 1162
TatI	WGTACW	4 cut(s)	90, 172, 485, 1140
TfiI	GAWTC	3 cut(s)	643, 1042, 1096
Tru1I	TTAA	4 cut(s)	68, 633, 782, 908
Tru9I	TTAA	4 cut(s)	68, 633, 782, 908
TscAI	CASTG	2 cut(s)	297, 819
TseFI	GTSAC	2 cut(s)	250, 947
TseI	GCWGC	1 cut(s)	498
Tsp45I	GTSAC	2 cut(s)	250, 947
TspDTI	ATGAA	8 cut(s)	33, 86, 342, 426, 591, 649, 653, 1088
TspGWI	ACGGA	2 cut(s)	335, 1097
TspRI	CASTG	2 cut(s)	297, 819
Van91I	CCANNNNNTGG	1 cut(s)	818
VpaK11BI	GGWCC	1 cut(s)	58
VspI	ATTAAT	1 cut(s)	68
XapI	RAATTY	3 cut(s)	465, 622, 1115
XcmI	CCANNNNNNNNNTGG	1 cut(s)	661
XspI	CTAG	1 cut(s)	525
ZrmI	AGTACT	1 cut(s)	1142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.