Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004536

Physical Location & Seq

Genomic Coordinates Reverse (-)

1122159 .. 1123583

Gene Structure 1425 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015741

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1143 bp

ATGGCTTCTTGTTTTGATGAACGAGTACCAAGCTGGACAGTACCAAAAAGAGAAGAGGTCCTCACATTAATAACCCTGAACAGCGCAATGTACACCAGACTGATGATGAATTATGAGGACGCTCCAATAGAAGATCGCAATCGTCACGAGGCCAAAGACCTCTCTATAGAGGAAGTACAGGAGAAACTTGAAACTCTGAAGAACAAATTAGACGACGGGAGCTTTAGTTGGATTTCACTAGACAAGTCCAACGTGACCTATGGTGAGTGTAGCGATGGCGTCAAACGTCTTACAGTGAAGGATATAAATAAACGCTCTAAACCAAGAGCTGAAGAAAGAGAAAAGATCCGGAATAATACCGCCAGTTCATTGTTCACACAAGACGAGGAGTACTACGCAATTCTTAATGACATCTGTTGCACTATAGGCTGCAGCAGTTCAAGTCTCTATATAGATGCTAGTGTAAGAGGCCTTGTTGGAGGTCTGCTTTCGTGGGTGCACAATGGGTACGAAACCGATTGTCGAAGCCAGGATGATGGGGTGGCCATCCCGTCCATGCATTCAGATATTAGTATGAAAAATTGTGGAGCCAAGTTCGTATTGGTGGTGGAAAATAAAGCCAGTTTTTATAATCTCATGCAAGACAGGTTTTACCGTGATTATCCATGCATCATCATCACCGGAATGGGAAAGCCCGGGGTGGCGACCAGAAGATTTTTAAAGCTATTGTCGGACAATTTTAGGCTTCCAGTGTATGGCCTTTTCGACTGTGATCCAGCAAGCATCAAAATGTTTTCGATTTATACTGTTGGGTCATATGAGGAGGCCTTCGATAGTGTAAACCTAACTTGCCCGTATATGACATGGTTGGGTGTTTGGCCGAGTGACCTGTGTGCGCTTGACATATCCTGCAACGATTTGAGTCCAAAAGAGATTAGAGAAATGGATAATCTTTTGAAAGAAGATTTTGTGAAAGACAATCCGAGGTTGGTTGAGGAGTTGAAACATATGAAAGAGACGGAGCAAAAAGGGAATCTTGATGCTTTGAAGGAGTTTGGGCCCACTTGTTTGTCAAAGTGCTATTTGCCATATAAGTTGAATTATATTTTAACCGCCGTCTGCCGTGAGAAGTGCACTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008134 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0030957 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046983 GO:0048285 GO:0050896 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

381

Amino Acids

43.36

Weight (kDa)

5.07

Isoelectric Point (pI)

46.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOP6A-Spo11_Toprim	PF21180	199 - 366	4.3e-47	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004536)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43731 FvH4_3g43753
rosa_chinensis	RchiOBHm_Chr2g0086091 RchiOBHm_Chr2g0089291 RchiOBHm_Chr2g0089851 RchiOBHm_Chr5g0083311
rosa_laevigata	RLG00000015741 RLG00000016061 RLG00000017209
rosa_multiflora	Rmu_co8293021.1_g000001 Rmu_co8360049.1_g000001 Rmu_sc0000388.1_g000047 Rmu_sc0002384.1_g000009 Rmu_sc0003382.1_g000021 Rmu_sc0017765.1_g000002 Rmu_ssc0000355.1_g000016
rosa_roxburghii	Rroxscaffold_2G00140180 Rroxscaffold_2G00151540
rosa_rugosa	Rorug01G0465600 Rorug01G0488800 Rorug02G0003700
rosa_samantha	Rh2AG015000 Rh2AG045100 Rh2AG048900 Rh2BG047800 Rh2CG016000 Rh2CG016100 Rh2CG045000 Rh2CG049900 Rh5BG561000 Rh5CG583600
rosa_wichuraiana	Rw2G001320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	630
AccB7I	CCANNNNNTGG	1 cut(s)	755
AccIII	TCCGGA	1 cut(s)	348
AciI	CCGC	2 cut(s)	360, 1113
AclWI	GGATC	2 cut(s)	340, 767
AcoI	YGGCCR	2 cut(s)	543, 878
AcuI	CTGAAG	2 cut(s)	218, 351
AcyI	GRCGYC	1 cut(s)	279
AfaI	GTAC	6 cut(s)	27, 42, 92, 177, 392, 509
AfiI	CCNNNNNNNGG	1 cut(s)	755
AgsI	TTSAA	6 cut(s)	191, 441, 958, 1003, 1048, 1099
AjnI	CCWGG	1 cut(s)	528
AluBI	AGCT	4 cut(s)	33, 222, 329, 724
AluI	AGCT	4 cut(s)	33, 222, 329, 724
Alw21I	GWGCWC	2 cut(s)	501, 1136
Alw26I	GTCTC	2 cut(s)	449, 1010
Alw44I	GTGCAC	2 cut(s)	497, 1132
AlwI	GGATC	2 cut(s)	340, 767
Ama87I	CYCGRG	1 cut(s)	695
Aor13HI	TCCGGA	1 cut(s)	348
AoxI	GGCC	7 cut(s)	150, 469, 543, 757, 825, 878, 1058
ApaI	GGGCCC	1 cut(s)	1062
ApaLI	GTGCAC	2 cut(s)	497, 1132
ApeKI	GCWGC	2 cut(s)	429, 432
AseI	ATTAAT	1 cut(s)	68
AspLEI	GCGC	2 cut(s)	86, 898
AspS9I	GGNCC	3 cut(s)	58, 1058, 1059
AsuC2I	CCSGG	2 cut(s)	696, 697
AsuHPI	GGTGA	2 cut(s)	275, 670
AvaI	CYCGRG	1 cut(s)	695
AvaII	GGWCC	1 cut(s)	58
BaeGI	GKGCMC	3 cut(s)	501, 1062, 1136
BalI	TGGCCA	1 cut(s)	545
BanII	GRGCYC	1 cut(s)	1062
BauI	CACGAG	1 cut(s)	146
Bbv12I	GWGCWC	2 cut(s)	501, 1136
BbvI	GCAGC	2 cut(s)	416, 444
BccI	CCATC	3 cut(s)	269, 530, 554
BceAI	ACGGC	2 cut(s)	1100, 1107
BciT130I	CCWGG	1 cut(s)	530
BcnI	CCSGG	2 cut(s)	696, 697
BcoDI	GTCTC	2 cut(s)	449, 1010
BfaI	CTAG	2 cut(s)	239, 459
BfmI	CTRYAG	3 cut(s)	165, 423, 430
BisI	GCNGC	2 cut(s)	430, 433
BlsI	GCNGC	2 cut(s)	431, 434
BmcAI	AGTACT	1 cut(s)	392
Bme1390I	CCNGG	3 cut(s)	530, 696, 697
Bme18I	GGWCC	1 cut(s)	58
BmeT110I	CYCGRG	1 cut(s)	695
BmgT120I	GGNCC	3 cut(s)	58, 1058, 1059
BmiI	GGNNCC	2 cut(s)	589, 1060
BmrFI	CCNGG	3 cut(s)	530, 696, 697
BmsI	GCATC	4 cut(s)	445, 678, 792, 1030
BpuMI	CCSGG	2 cut(s)	696, 697
BsaBI	GATNNNNATC	1 cut(s)	138
BsaHI	GRCGYC	1 cut(s)	279
BsaJI	CCNNGG	3 cut(s)	695, 696, 983
BsaWI	WCCGGW	2 cut(s)	348, 680
BsaXI	ACNNNNNCTCC	2 cut(s)	211, 241
Bsc4I	CCNNNNNNNGG	1 cut(s)	755
Bse1I	ACTGG	3 cut(s)	363, 621, 749
Bse3DI	GCAATG	1 cut(s)	93
Bse8I	GATNNNNATC	1 cut(s)	138
BseAI	TCCGGA	1 cut(s)	348
BseBI	CCWGG	1 cut(s)	530
BseDI	CCNNGG	3 cut(s)	695, 696, 983
BseGI	GGATG	2 cut(s)	538, 546
BseJI	GATNNNNATC	1 cut(s)	138
BseLI	CCNNNNNNNGG	1 cut(s)	755
BseMI	GCAATG	1 cut(s)	93
BseNI	ACTGG	3 cut(s)	363, 621, 749
BseRI	GAGGAG	3 cut(s)	401, 836, 1010
BseSI	GKGCMC	3 cut(s)	501, 1062, 1136
BseXI	GCAGC	2 cut(s)	416, 444
BshFI	GGCC	7 cut(s)	152, 471, 545, 759, 827, 880, 1060
BsiHKAI	GWGCWC	2 cut(s)	501, 1136
BsiHKCI	CYCGRG	1 cut(s)	695
BsiSI	CCGG	3 cut(s)	349, 681, 696
BslI	CCNNNNNNNGG	1 cut(s)	755
BsmAI	GTCTC	2 cut(s)	449, 1010
BsmBI	CGTCTC	1 cut(s)	1010
BsmI	GAATGC	1 cut(s)	559
BsnI	GGCC	7 cut(s)	152, 471, 545, 759, 827, 880, 1060
BsoBI	CYCGRG	1 cut(s)	695
Bsp120I	GGGCCC	1 cut(s)	1058
Bsp1286I	GDGCHC	3 cut(s)	501, 1062, 1136
Bsp13I	TCCGGA	1 cut(s)	348
Bsp1407I	TGTACA	1 cut(s)	90
Bsp143I	GATC	3 cut(s)	133, 345, 772
BspACI	CCGC	2 cut(s)	360, 1113
BspANI	GGCC	7 cut(s)	152, 471, 545, 759, 827, 880, 1060
BspEI	TCCGGA	1 cut(s)	348
BspLI	GGNNCC	2 cut(s)	589, 1060
BspMAI	CTGCAG	1 cut(s)	434
BspPI	GGATC	2 cut(s)	340, 767
BsrDI	GCAATG	1 cut(s)	93
BsrGI	TGTACA	1 cut(s)	90
BsrI	ACTGG	3 cut(s)	363, 621, 749
BssECI	CCNNGG	3 cut(s)	695, 696, 983
BssMI	GATC	3 cut(s)	133, 345, 772
BssNI	GRCGYC	1 cut(s)	279
BssSI	CACGAG	1 cut(s)	146
Bst2BI	CACGAG	1 cut(s)	146
Bst2UI	CCWGG	1 cut(s)	530
Bst4CI	ACNGT	5 cut(s)	40, 295, 656, 770, 808
Bst6I	CTCTTC	1 cut(s)	48
BstACI	GRCGYC	1 cut(s)	279
BstAUI	TGTACA	1 cut(s)	90
BstC8I	GCNNGC	1 cut(s)	781
BstF5I	GGATG	2 cut(s)	538, 546
BstHHI	GCGC	2 cut(s)	86, 898
BstKTI	GATC	3 cut(s)	136, 348, 775
BstMAI	GTCTC	2 cut(s)	449, 1010
BstMBI	GATC	3 cut(s)	133, 345, 772
BstMWI	GCNNNNNNNGC	1 cut(s)	426
BstNI	CCWGG	1 cut(s)	530
BstSCI	CCNGG	3 cut(s)	528, 694, 695
BstSFI	CTRYAG	3 cut(s)	165, 423, 430
BstSLI	GKGCMC	3 cut(s)	501, 1062, 1136
BstV1I	GCAGC	2 cut(s)	416, 444
BstX2I	RGATCY	1 cut(s)	345
BstXI	CCANNNNNNTGG	1 cut(s)	536
BstYI	RGATCY	1 cut(s)	345
BsuRI	GGCC	7 cut(s)	152, 471, 545, 759, 827, 880, 1060
BtgZI	GCGATG	1 cut(s)	288
BtsCI	GGATG	2 cut(s)	538, 546
BtsIMutI	CAGTG	3 cut(s)	300, 756, 1134
Cac8I	GCNNGC	1 cut(s)	781
CfoI	GCGC	2 cut(s)	86, 898
Cfr13I	GGNCC	3 cut(s)	58, 1058, 1059
Cfr9I	CCCGGG	1 cut(s)	695
CseI	GACGC	2 cut(s)	128, 268
Csp6I	GTAC	6 cut(s)	26, 41, 91, 176, 391, 508
CviAII	CATG	4 cut(s)	556, 637, 666, 864
CviQI	GTAC	6 cut(s)	26, 41, 91, 176, 391, 508
DpnI	GATC	3 cut(s)	135, 347, 774
DpnII	GATC	3 cut(s)	133, 345, 772
DraI	TTTAAA	1 cut(s)	720
EaeI	YGGCCR	2 cut(s)	543, 878
Eam1104I	CTCTTC	1 cut(s)	48
EarI	CTCTTC	1 cut(s)	48
Eco147I	AGGCCT	2 cut(s)	471, 827
Eco24I	GRGCYC	1 cut(s)	1062
Eco47I	GGWCC	1 cut(s)	58
Eco57I	CTGAAG	2 cut(s)	218, 351
Eco88I	CYCGRG	1 cut(s)	695
EcoO109I	RGGNCCY	1 cut(s)	58
EcoRII	CCWGG	1 cut(s)	528
EcoT22I	ATGCAT	2 cut(s)	561, 671
EcoT38I	GRGCYC	1 cut(s)	1062
Esp3I	CGTCTC	1 cut(s)	1010
FaeI	CATG	4 cut(s)	559, 640, 669, 867
FalI	AAGNNNNNCTT	2 cut(s)	1020, 1052
FatI	CATG	4 cut(s)	555, 636, 665, 863
FauNDI	CATATG	2 cut(s)	817, 1008
Fnu4HI	GCNGC	2 cut(s)	430, 433
FokI	GGATG	2 cut(s)	533, 545
FriOI	GRGCYC	1 cut(s)	1062
Fsp4HI	GCNGC	2 cut(s)	430, 433
FspBI	CTAG	2 cut(s)	239, 459
GlaI	GCGC	2 cut(s)	85, 897
GluI	GCNGC	2 cut(s)	430, 433
HaeIII	GGCC	7 cut(s)	152, 471, 545, 759, 827, 880, 1060
HapII	CCGG	3 cut(s)	349, 681, 696
HgaI	GACGC	2 cut(s)	128, 268
HhaI	GCGC	2 cut(s)	86, 898
Hin1I	GRCGYC	1 cut(s)	279
Hin1II	CATG	4 cut(s)	559, 640, 669, 867
Hin6I	GCGC	2 cut(s)	84, 896
HinP1I	GCGC	2 cut(s)	84, 896
HinfI	GANTC	2 cut(s)	922, 1033
HpaII	CCGG	3 cut(s)	349, 681, 696
HphI	GGTGA	2 cut(s)	275, 670
Hpy166II	GTNNAC	5 cut(s)	93, 375, 499, 841, 1134
Hpy188I	TCNGA	4 cut(s)	198, 565, 733, 984
Hpy188III	TCNNGA	3 cut(s)	146, 349, 1037
Hpy8I	GTNNAC	5 cut(s)	93, 375, 499, 841, 1134
Hpy99I	CGWCG	1 cut(s)	218
HpyAV	CCTTC	3 cut(s)	292, 838, 1042
HpyCH4III	ACNGT	5 cut(s)	40, 295, 656, 770, 808
HpyCH4IV	ACGT	2 cut(s)	252, 286
HpyCH4V	TGCA	8 cut(s)	420, 432, 499, 559, 640, 669, 912, 1134
HpyF10VI	GCNNNNNNNGC	1 cut(s)	426
HpySE526I	ACGT	2 cut(s)	252, 286
Hsp92I	GRCGYC	1 cut(s)	279
Hsp92II	CATG	4 cut(s)	559, 640, 669, 867
HspAI	GCGC	2 cut(s)	84, 896
Kpn2I	TCCGGA	1 cut(s)	348
Kzo9I	GATC	3 cut(s)	133, 345, 772
LmnI	GCTCC	4 cut(s)	127, 219, 587, 1021
Lsp1109I	GCAGC	2 cut(s)	416, 444
LweI	GCATC	4 cut(s)	445, 678, 792, 1030
MaeI	CTAG	2 cut(s)	239, 459
MaeII	ACGT	2 cut(s)	252, 286
MaeIII	GTNAC	3 cut(s)	143, 253, 884
MalI	GATC	3 cut(s)	135, 347, 774
MboI	GATC	3 cut(s)	133, 345, 772
MboII	GAAGA	6 cut(s)	65, 143, 211, 344, 723, 974
MflI	RGATCY	1 cut(s)	345
MhlI	GDGCHC	3 cut(s)	501, 1062, 1136
MlsI	TGGCCA	1 cut(s)	545
MluCI	AATT	6 cut(s)	109, 206, 399, 580, 736, 1099
MluNI	TGGCCA	1 cut(s)	545
MlyI	GAGTC	1 cut(s)	931
MmeI	TCCRAC	4 cut(s)	209, 273, 457, 711
Mox20I	TGGCCA	1 cut(s)	545
Mph1103I	ATGCAT	2 cut(s)	561, 671
MroI	TCCGGA	1 cut(s)	348
MscI	TGGCCA	1 cut(s)	545
MseI	TTAA	5 cut(s)	68, 405, 719, 1109, 1141
MslI	CAYNNNNRTG	2 cut(s)	683, 788
Msp20I	TGGCCA	1 cut(s)	545
MspI	CCGG	3 cut(s)	349, 681, 696
MspR9I	CCNGG	3 cut(s)	530, 696, 697
Mva1269I	GAATGC	1 cut(s)	559
MvaI	CCWGG	1 cut(s)	530
MwoI	GCNNNNNNNGC	1 cut(s)	426
NciI	CCSGG	2 cut(s)	696, 697
NdeI	CATATG	2 cut(s)	817, 1008
NdeII	GATC	3 cut(s)	133, 345, 772
NlaIII	CATG	4 cut(s)	559, 640, 669, 867
NlaIV	GGNNCC	2 cut(s)	589, 1060
NmeAIII	GCCGAG	1 cut(s)	906
NmuCI	GTSAC	3 cut(s)	143, 253, 884
NsiI	ATGCAT	2 cut(s)	561, 671
PceI	AGGCCT	2 cut(s)	471, 827
PctI	GAATGC	1 cut(s)	559
PfeI	GAWTC	1 cut(s)	1033
PflMI	CCANNNNNTGG	1 cut(s)	755
PkrI	GCNGC	2 cut(s)	431, 434
PleI	GAGTC	1 cut(s)	930
PpsI	GAGTC	1 cut(s)	930
PpuMI	RGGWCCY	1 cut(s)	58
PshBI	ATTAAT	1 cut(s)	68
PsiI	TTATAA	1 cut(s)	630
Psp5II	RGGWCCY	1 cut(s)	58
Psp6I	CCWGG	1 cut(s)	528
PspGI	CCWGG	1 cut(s)	528
PspN4I	GGNNCC	2 cut(s)	589, 1060
PspOMI	GGGCCC	1 cut(s)	1058
PspPI	GGNCC	3 cut(s)	58, 1058, 1059
PspPPI	RGGWCCY	1 cut(s)	58
PstI	CTGCAG	1 cut(s)	434
PsuI	RGATCY	1 cut(s)	345
RsaI	GTAC	6 cut(s)	27, 42, 92, 177, 392, 509
RsaNI	GTAC	6 cut(s)	26, 41, 91, 176, 391, 508
RseI	CAYNNNNRTG	2 cut(s)	683, 788
SaqAI	TTAA	5 cut(s)	68, 405, 719, 1109, 1141
SatI	GCNGC	2 cut(s)	430, 433
Sau3AI	GATC	3 cut(s)	133, 345, 772
Sau96I	GGNCC	3 cut(s)	58, 1058, 1059
ScaI	AGTACT	1 cut(s)	392
SchI	GAGTC	1 cut(s)	931
ScrFI	CCNGG	3 cut(s)	530, 696, 697
SduI	GDGCHC	3 cut(s)	501, 1062, 1136
SfaNI	GCATC	4 cut(s)	445, 678, 792, 1030
SfcI	CTRYAG	3 cut(s)	165, 423, 430
SinI	GGWCC	1 cut(s)	58
SmaI	CCCGGG	1 cut(s)	697
SmiMI	CAYNNNNRTG	2 cut(s)	683, 788
Sse9I	AATT	6 cut(s)	109, 206, 399, 580, 736, 1099
SseBI	AGGCCT	2 cut(s)	471, 827
SsiI	CCGC	2 cut(s)	360, 1113
SspMI	CTAG	2 cut(s)	239, 459
StuI	AGGCCT	2 cut(s)	471, 827
StyD4I	CCNGG	3 cut(s)	528, 694, 695
TaaI	ACNGT	5 cut(s)	40, 295, 656, 770, 808
TaiI	ACGT	2 cut(s)	255, 289
TaqI	TCGA	4 cut(s)	523, 765, 797, 831
TasI	AATT	6 cut(s)	109, 206, 399, 580, 736, 1099
TatI	WGTACW	3 cut(s)	90, 175, 390
TfiI	GAWTC	1 cut(s)	1033
Tru1I	TTAA	5 cut(s)	68, 405, 719, 1109, 1141
Tru9I	TTAA	5 cut(s)	68, 405, 719, 1109, 1141
TscAI	CASTG	3 cut(s)	300, 756, 1141
TseFI	GTSAC	3 cut(s)	143, 253, 884
TseI	GCWGC	2 cut(s)	429, 432
Tsp45I	GTSAC	3 cut(s)	143, 253, 884
TspDTI	ATGAA	5 cut(s)	33, 122, 357, 590, 1025
TspGWI	ACGGA	1 cut(s)	1034
TspMI	CCCGGG	1 cut(s)	695
TspRI	CASTG	3 cut(s)	300, 756, 1141
Van91I	CCANNNNNTGG	1 cut(s)	755
VneI	GTGCAC	2 cut(s)	497, 1132
VpaK11BI	GGWCC	1 cut(s)	58
VspI	ATTAAT	1 cut(s)	68
XcmI	CCANNNNNNNNNTGG	1 cut(s)	598
XmaI	CCCGGG	1 cut(s)	695
XspI	CTAG	2 cut(s)	239, 459
ZrmI	AGTACT	1 cut(s)	392
Zsp2I	ATGCAT	2 cut(s)	561, 671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.