FvH4_3g44030

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015588
Physical Location & Seq
Reverse (-)
36732707 .. 36739529
6823 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44030.t1

Sequence Viewer

Length: 3672 bp
ATGGAGTTCTCTTCCATGGAGATGGCTTTGGCGATAGCTGTTGGATTTGCTACAGTGACACATTGGTTGGCTTCCATGGTTGAAAACCCTGAGCCAGTGTCTAATGATAATCGAGAATTGGAAGACGTGGATCCCCCTGCATCTCTTCTTTCATCAGCTGAATCATCGTCAGCTCCCATGGTTGAAAACCCTGAGCCAAAATCAGAGGATAATCAAGAATCAGTTGTAATGGATGCCTCTACATCTCTTCTTTCATCAGCTCCTTGGAAGAAGTATGATGTATTTCTGAGTTTCAGGGGTGCAGACACTCGTACGACTTTTCTATCCCACTTACACCATGAATTGACATATGTGGAAGGGATTCAAACGTTCATGGATGACAAAGAGCTTGAACTAGGGGCTCCTGTTGCTCCACGTCTGCTAAAGGCAATTGAAGAATCAAATTTTGCAATTGTTGTTCTGTCAAAAAACTATGCTCAATCTATATGGTGTTTGGAGGAACTTACAAAGATTTGTCAATGCATGATAAACAATAACAGAATTCTACCACTTTTTTATCATGTCGAACCTACTGATGTGCGGTATCAAAAGGGGACTTTTGGTGATGCTTTCACTGAGCTTGAAAACTCTGGTCGGCACAATTTAGAGAAGGTGCAGCAGTGGAGAAATGCTTTACACAAAGTGGCCGGTTTCTCTGGCTGTAATGCAAAGGATCACAAGACTGATAGAGAAGTTGTCAAAGCCATTGTTGAATCTGTGTGCAGTAAAGTACGACACAGTGAAATTGAGCACATTATTTCCTCCAATGATTTTCAACCTTTTGAAGCAACAAAAAAAGCCATGAATGAGCTTATGAAGGAGCTAAGAAATGAACACGTCAATATCGTCGGGGTCTACGGCATGGGAGGTGTTGGAAAGTCAACAATGGTGGAACAAGTTGGTGCACAAGCCGAAAAAGAAGGGCTTTTCCATAAGGTTATTTTGGCTTGCATATCCCCAGAACCTAATCAGTGGAATATTCAAGGCACATTAGCAGACTTGTTGGGTTTGAAGCTGGTGGATCAGACAGTAATTGGAAGAGCCCGTACATTGAGGAAGAAGATAACAAGTGGAAATAAGATCCTTATAATCCTGGACAACATTTGGAATGATATGGACTTGACAACCATAGGAATTCCCAGATACAATGAGCTTAAAAGATGTTATTCCAAGGTCTTAATCACCACAAGGAAGAGGGAAGTTTGCTATCGTATGCATTGCCAAGCTAGCATACATCTCAGTATCTTAACTGAAGAGGATTCTTGGAAGTTGTTTGTGACGGAAGCACGAAAGTCTTTTGGAACATCAACTTTCTATGACAAAGCAAGGGAGGTAGCAAGAGAATGTGCTGGTCTTCCAGTTGCGTTGAAAGCAGTTGCAAAGGCACTTGGGGATAAAGACTTGCGTGAATGGGAAAAGGCTGCTGAGCGACTAAAGATGTCTCAACTTCCCCTCCCTGAAGACGAGAGAGATGTGTTCAAATGCATCAAGTTAAGCTATGATTACTTGAAATCTGATGTTTCCAAATCATGCTTCTTGCTTTGCTGCCTATTCCCGGAAGATGATGATATCAAAATTGAAGACTTGCTGAAGTATGGGATTGGGAAAGGATTGTTTGAAGAGTCCAACGTGCAAAATGCCAGAGACACAATACATACGGTGGTTGAGTCTCTTAAAGATTCTAGCCTGCTTTTGGATGCTAAAAAAGAGGGGGGATACATAAGGATGCATGATGTCGTCAGAGATGTTGCCATACAAATTTCCTCACAATTTTTTGTAAGAGCTGGTCTTGAATTGCTTCATTGGCCGGACATTAATGCACGTAAAGGCTACATTGCAATCTCACTAATGAAGAACAAAATACGCACGATTCCCGCTGAACAGCTGGTATTTCCAAAACTTCATATTCTCTTGTTACAAAGTAATATCATGAAGAACATCCCAGTTTCTTCATTTGAACGTCTAGAGGCGTTAAGGGTGTTAGATCTTAGCTACACTGGCATTTCTTTACTTCCAGCATCGTTCAATCTCCTAAGCAGTCTCCACACTTTGTATCTAGATGGTTGCTTGTCAAGGATAAGTAACATATCCCTACTTGGACAGTTGAAAAAACTTGAAATTCTTAGCATGAGAGACTTTCCTCTTTTGAAATTGCCGAAAGAAATAGGACAGTTGACCGCACTAAGGATGTTGGACTTTACTAGAGTCTGTTTTGATACAATTCCTTCTAAAGTGATAAGCAACCTCAATAAATTAGAGGAACTGTATATACAGTGCAATTTCGCAGACTGGGGGAGTAAAGTTAAGGGGAGTATAACAGAGGGGAGTGAAATCCAGGGAAATACAGTTGAGGAGAGTGAAGCTGAGGGAGCAAGAGAGGAAACTAATGCTTCCTTTGATGAGTTAACTAGCTTGTTGCATCTACACATTTTGAAGGTTTTCATATCTGATCCAAAATGCTTACCTACAGATGAGGTTAAGTCTGACTGGAAAAGTTTTGACATATGTGTCTGTGAAGACCTGTCAATTAGAAAATCGCTTTTGAATTCATGTTCTCCACTTGATTCAAAAGCCTTGAATTCAAGTTCTTCACTAGATTCAAAAACCTTGACTCTTGATACAACCATGGATACCTTACCGAAATGGTTTACTGATGTGGTGACCAAGAAAACAGAGAAGCTACAGTATAGAAACTGTCGAGGTTTAGTTAATGTTCTCGAGGAATATTACCAAGGGACATTAAGTGCACTTAAGGATTTACAGGTTATTGGTCCCAGTGAGAATTTGGAACAATTGATGACCTCAATAACATCGGTTCCCGATGAACCTGTATTTGAAAATTTGGAAGAGTTGCATCTTGTTGAAGTGAATTGTCTGAAGGAGTTATGTGTTGGTGAATTGCCAAATGAGTCTCTATGCAAGCTTAAATTACTGAAGGTGAGAGGTTGTCATAAGTTGATGAATGCACTTTTACAATCTAATTTGTTGAAGAGACTACACAATTTGGAAAAACTAAGTTGTGAAGAGATGGATAAATTAGAGTATATATTTTCATCTGAAAGGTCTGAGCTAGAAGAAAGCACTCTGCCAGAGTTGATAGAGATGAGATTGCAGAATCTTGGGAACCTAATAAAGATATGGAAAGGTCCTGCTCCATGTGGAATCTTTCATCATGTAAAATTTTTGGTAGTTACCGAGTGCAATAAGCTGAAAAATCTCTTCACATCCGACGTAGCTCACCATCTTCATGACTTGGAAGATCTTTCAGTAGAGGCTTGCCGAGGGTTGGAGAGAGTCATTGAAGCAAGTGAGGAAACAGTGCACAACAAGATAGTTCTACCAAAATTGAAGAAGTTAGCTTTAAAAATGCTTCTTAAGCTTACAAGGTTCTATGGTAGTACTGGACATGCTGCTAATGATGAAGATGAAGAAAATATTGAGTTTCCTTTATTGGAACACTCAGATGTGCAACGCTGCAGTAGATTTAGATCTCCAGCTAAGAAGAAGTTGGTTACAATGATGAATAAGCAGCATGTTCTGATTCTTTCGCGAGAGACAGGTCAGAAAGATATACCTCTTTTAAGCAGAAGAAGAGGAGAGGTTCCGAGAGAAAGAAGGTACATCCATCAATAG

Protein Analysis

1224

Amino Acids

138.49

Weight (kDa)

5.94

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 91 - 260 9.6e-47 TIR domain
TIR_2 PF13676 94 - 190 1.2e-13 TIR domain
NB-ARC PF00931 278 - 442 1.2e-27 NB-ARC domain
LRR_8 PF13855 645 - 702 9e-09 Leucine rich repeat
LRR_14 PF23598 697 - 899 8.9e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 960 - 1107 3.1e-26 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1045 - 1177 1.6e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1127
AasI GACNNNNNNGTC 1 cut(s) 2546
AccI GTMKAC 1 cut(s) 894
AccII CGCG 1 cut(s) 3589
AciI CCGC 3 cut(s) 580, 1914, 2217
AclI AACGTT 1 cut(s) 368
AclWI GGATC 6 cut(s) 125, 138, 720, 1068, 1114, 2483
AcoI YGGCCR 2 cut(s) 684, 1844
AcuI CTGAAG 5 cut(s) 1311, 1518, 1649, 2936, 2993
AdeI CACNNNGTG 1 cut(s) 682
AfaI GTAC 5 cut(s) 313, 771, 1087, 3439, 3659
AfiI CCNNNNNNNGG 4 cut(s) 1594, 1732, 2223, 3325
AflII CTTAAG 2 cut(s) 2789, 3413
AflIII ACRYGT 1 cut(s) 874
AjiI CACGTC 3 cut(s) 127, 416, 877
AjnI CCWGG 2 cut(s) 1131, 2373
AjuI GAANNNNNNNTTGG 2 cut(s) 1556, 1588
Alw21I GWGCWC 4 cut(s) 792, 946, 2788, 3363
Alw26I GTCTC 8 cut(s) 1485, 1677, 1713, 2084, 2166, 2955, 3025, 3587
Alw44I GTGCAC 3 cut(s) 942, 2784, 3359
AlwI GGATC 6 cut(s) 125, 138, 720, 1068, 1114, 2483
Ama87I CYCGRG 1 cut(s) 2756
AoxI GGCC 2 cut(s) 684, 1844
ApaLI GTGCAC 3 cut(s) 942, 2784, 3359
ApeKI GCWGC 6 cut(s) 655, 1460, 1584, 3449, 3513, 3568
AseI ATTAAT 1 cut(s) 1854
Asp700I GAANNNNTTC 3 cut(s) 360, 1836, 2477
AspS9I GGNCC 2 cut(s) 2810, 3185
AsuC2I CCSGG 1 cut(s) 1595
AsuHPI GGTGA 6 cut(s) 614, 1213, 2710, 2945, 2989, 3269
AsuNHI GCTAGC 1 cut(s) 1265
AvaI CYCGRG 1 cut(s) 2756
AvaII GGWCC 2 cut(s) 2810, 3185
BaeGI GKGCMC 3 cut(s) 946, 2788, 3363
BamHI GGATCC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 403, 1084
BarI GAAGNNNNNNTAC 2 cut(s) 1069, 1101
BbsI GAAGAC 5 cut(s) 129, 1385, 1506, 1626, 2562
Bbv12I GWGCWC 4 cut(s) 792, 946, 2788, 3363
BbvCI CCTCAGC 1 cut(s) 2403
BbvI GCAGC 6 cut(s) 667, 1447, 1571, 3436, 3500, 3580
BccI CCATC 4 cut(s) 16, 2093, 3061, 3288
BceAI ACGGC 1 cut(s) 913
BciT130I CCWGG 2 cut(s) 1133, 2375
BciVI GTATCC 2 cut(s) 1748, 2662
BcnI CCSGG 1 cut(s) 1595
BcoDI GTCTC 8 cut(s) 1485, 1677, 1713, 2084, 2166, 2955, 3025, 3587
BfaI CTAG 9 cut(s) 395, 1266, 1722, 2003, 2096, 2241, 2448, 2633, 3110
BfmI CTRYAG 4 cut(s) 51, 2505, 2720, 3514
BfrI CTTAAG 2 cut(s) 2789, 3413
BfuI GTATCC 2 cut(s) 1748, 2662
BglII AGATCT 3 cut(s) 2023, 3298, 3527
BisI GCNGC 6 cut(s) 656, 1461, 1585, 3450, 3514, 3569
BlpI GCTNAGC 1 cut(s) 1464
BlsI GCNGC 6 cut(s) 657, 1462, 1586, 3451, 3515, 3570
BmcAI AGTACT 1 cut(s) 3439
Bme1390I CCNGG 3 cut(s) 1133, 1595, 2375
Bme18I GGWCC 2 cut(s) 2810, 3185
BmeT110I CYCGRG 1 cut(s) 2756
BmgBI CACGTC 3 cut(s) 127, 416, 877
BmgT120I GGNCC 2 cut(s) 2810, 3185
BmiI GGNNCC 6 cut(s) 132, 402, 2812, 2856, 3164, 3642
BmrFI CCNGG 3 cut(s) 1133, 1595, 2375
BmrI ACTGGG 3 cut(s) 1976, 2338, 2808
BmsI GCATC 9 cut(s) 149, 223, 595, 1533, 1726, 1755, 2066, 2467, 2902
BmtI GCTAGC 1 cut(s) 1269
BmuI ACTGGG 3 cut(s) 1976, 2338, 2808
BpiI GAAGAC 5 cut(s) 129, 1385, 1506, 1626, 2562
BpmI CTGGAG 1 cut(s) 3516
Bpu10I CCTNAGC 4 cut(s) 90, 192, 2072, 2403
Bpu1102I GCTNAGC 1 cut(s) 1464
BpuMI CCSGG 1 cut(s) 1595
BsaAI YACGTR 1 cut(s) 1862
BsaBI GATNNNNATC 1 cut(s) 3526
BsaJI CCNNGG 9 cut(s) 15, 75, 177, 263, 1209, 2374, 2664, 2770, 3319
BsaXI ACNNNNNCTCC 2 cut(s) 1476, 1506
Bsc4I CCNNNNNNNGG 4 cut(s) 1594, 1732, 2223, 3325
Bse118I RCCGGY 1 cut(s) 686
Bse1I ACTGG 8 cut(s) 95, 1397, 1982, 2041, 2333, 2531, 2814, 3445
Bse3DI GCAATG 2 cut(s) 1255, 1872
Bse8I GATNNNNATC 1 cut(s) 3526
BseBI CCWGG 2 cut(s) 1133, 2375
BseDI CCNNGG 9 cut(s) 15, 75, 177, 263, 1209, 2374, 2664, 2770, 3319
BseGI GGATG 8 cut(s) 238, 382, 1741, 1770, 1977, 2232, 3263, 3660
BseJI GATNNNNATC 1 cut(s) 3526
BseLI CCNNNNNNNGG 4 cut(s) 1594, 1732, 2223, 3325
BseMI GCAATG 2 cut(s) 1255, 1872
BseMII CTCAG 9 cut(s) 81, 183, 278, 606, 1291, 1455, 2394, 3096, 3513
BseNI ACTGG 8 cut(s) 95, 1397, 1982, 2041, 2333, 2531, 2814, 3445
BseRI GAGGAG 2 cut(s) 2405, 3648
BseSI GKGCMC 3 cut(s) 946, 2788, 3363
BseXI GCAGC 6 cut(s) 667, 1447, 1571, 3436, 3500, 3580
BsgI GTGCAG 3 cut(s) 321, 674, 781
Bsh1236I CGCG 1 cut(s) 3589
BshFI GGCC 2 cut(s) 686, 1846
BsiHKAI GWGCWC 4 cut(s) 792, 946, 2788, 3363
BsiHKCI CYCGRG 1 cut(s) 2756
BsiSI CCGG 3 cut(s) 687, 1595, 1847
BsiWI CGTACG 1 cut(s) 311
BslFI GGGAC 3 cut(s) 607, 2788, 2796
BslI CCNNNNNNNGG 4 cut(s) 1594, 1732, 2223, 3325
BsmAI GTCTC 8 cut(s) 1485, 1677, 1713, 2084, 2166, 2955, 3025, 3587
BsmFI GGGAC 3 cut(s) 607, 2788, 2796
BsmI GAATGC 1 cut(s) 3007
BsnI GGCC 2 cut(s) 686, 1846
BsoBI CYCGRG 1 cut(s) 2756
Bsp1286I GDGCHC 6 cut(s) 403, 792, 946, 1084, 2788, 3363
Bsp143I GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
Bsp1720I GCTNAGC 1 cut(s) 1464
Bsp19I CCATGG 4 cut(s) 15, 75, 177, 2664
Bsp68I TCGCGA 1 cut(s) 3589
BspACI CCGC 3 cut(s) 580, 1914, 2217
BspANI GGCC 2 cut(s) 686, 1846
BspCNI CTCAG 9 cut(s) 82, 184, 279, 607, 1290, 1456, 2395, 3097, 3512
BspFNI CGCG 1 cut(s) 3589
BspHI TCATGA 2 cut(s) 1968, 3286
BspLI GGNNCC 6 cut(s) 132, 402, 2812, 2856, 3164, 3642
BspMAI CTGCAG 1 cut(s) 3518
BspOI GCTAGC 1 cut(s) 1269
BspPI GGATC 6 cut(s) 125, 138, 720, 1068, 1114, 2483
BspQI GCTCTTC 1 cut(s) 1072
BspTI CTTAAG 2 cut(s) 2789, 3413
BsrDI GCAATG 2 cut(s) 1255, 1872
BsrFI RCCGGY 1 cut(s) 686
BsrI ACTGG 8 cut(s) 95, 1397, 1982, 2041, 2333, 2531, 2814, 3445
BssAI RCCGGY 1 cut(s) 686
BssECI CCNNGG 9 cut(s) 15, 75, 177, 263, 1209, 2374, 2664, 2770, 3319
BssMI GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
BssT1I CCWWGG 7 cut(s) 15, 75, 177, 263, 1209, 2664, 2770
Bst2UI CCWGG 2 cut(s) 1133, 2375
BstAFI CTTAAG 2 cut(s) 2789, 3413
BstBAI YACGTR 1 cut(s) 1862
BstC8I GCNNGC 5 cut(s) 988, 1267, 1727, 2960, 3316
BstDSI CCRYGG 4 cut(s) 15, 75, 177, 2664
BstEII GGTNACC 1 cut(s) 2698
BstF5I GGATG 8 cut(s) 238, 382, 1741, 1770, 1977, 2232, 3263, 3660
BstFNI CGCG 1 cut(s) 3589
BstKTI GATC 8 cut(s) 133, 715, 1063, 1122, 2026, 2491, 3301, 3530
BstMAI GTCTC 8 cut(s) 1485, 1677, 1713, 2084, 2166, 2955, 3025, 3587
BstMBI GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
BstMWI GCNNNNNNNGC 7 cut(s) 407, 1266, 1409, 1843, 2037, 2408, 3415
BstNI CCWGG 2 cut(s) 1133, 2375
BstNSI RCATGY 2 cut(s) 3449, 3575
BstPI GGTNACC 1 cut(s) 2698
BstSCI CCNGG 3 cut(s) 1131, 1593, 2373
BstSFI CTRYAG 4 cut(s) 51, 2505, 2720, 3514
BstSLI GKGCMC 3 cut(s) 946, 2788, 3363
BstUI CGCG 1 cut(s) 3589
BstV1I GCAGC 6 cut(s) 667, 1447, 1571, 3436, 3500, 3580
BstV2I GAAGAC 5 cut(s) 129, 1385, 1506, 1626, 2562
BstX2I RGATCY 5 cut(s) 130, 1119, 2023, 3298, 3527
BstXI CCANNNNNNTGG 1 cut(s) 22
BstYI RGATCY 5 cut(s) 130, 1119, 2023, 3298, 3527
BsuI GTATCC 2 cut(s) 1748, 2662
BsuRI GGCC 2 cut(s) 686, 1846
BtgI CCRYGG 4 cut(s) 15, 75, 177, 2664
BtrI CACGTC 3 cut(s) 127, 416, 877
BtsCI GGATG 8 cut(s) 238, 382, 1741, 1770, 1977, 2232, 3263, 3660
BtsI GCAGTG 1 cut(s) 665
BtuMI TCGCGA 1 cut(s) 3589
Cac8I GCNNGC 5 cut(s) 988, 1267, 1727, 2960, 3316
CciI TCATGA 2 cut(s) 1968, 3286
Cfr10I RCCGGY 1 cut(s) 686
Cfr13I GGNCC 2 cut(s) 2810, 3185
Csp6I GTAC 5 cut(s) 312, 770, 1086, 3438, 3658
CspCI CAANNNNNGTGG 2 cut(s) 909, 944
CviQI GTAC 5 cut(s) 312, 770, 1086, 3438, 3658
DpnI GATC 8 cut(s) 132, 714, 1062, 1121, 2025, 2490, 3300, 3529
DpnII GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
DraI TTTAAA 1 cut(s) 3402
DraIII CACNNNGTG 1 cut(s) 682
DrdI GACNNNNNNGTC 1 cut(s) 2546
DseDI GACNNNNNNGTC 1 cut(s) 2546
EaeI YGGCCR 2 cut(s) 684, 1844
Eco130I CCWWGG 7 cut(s) 15, 75, 177, 263, 1209, 2664, 2770
Eco24I GRGCYC 2 cut(s) 403, 1084
Eco32I GATATC 1 cut(s) 1609
Eco47I GGWCC 2 cut(s) 2810, 3185
Eco57I CTGAAG 5 cut(s) 1311, 1518, 1649, 2936, 2993
Eco88I CYCGRG 1 cut(s) 2756
Eco91I GGTNACC 1 cut(s) 2698
EcoO109I RGGNCCY 1 cut(s) 3185
EcoO65I GGTNACC 1 cut(s) 2698
EcoRI GAATTC 4 cut(s) 540, 1173, 2584, 2617
EcoRII CCWGG 2 cut(s) 1131, 2373
EcoRV GATATC 1 cut(s) 1609
EcoT14I CCWWGG 7 cut(s) 15, 75, 177, 263, 1209, 2664, 2770
EcoT22I ATGCAT 4 cut(s) 524, 1257, 1526, 1770
EcoT38I GRGCYC 2 cut(s) 403, 1084
ErhI CCWWGG 7 cut(s) 15, 75, 177, 263, 1209, 2664, 2770
FalI AAGNNNNNCTT 2 cut(s) 948, 980
FaqI GGGAC 3 cut(s) 607, 2788, 2796
FauI CCCGC 1 cut(s) 1921
FauNDI CATATG 2 cut(s) 349, 2543
FblI GTMKAC 1 cut(s) 894
Fnu4HI GCNGC 6 cut(s) 656, 1461, 1585, 3450, 3514, 3569
FokI GGATG 8 cut(s) 245, 389, 1748, 1777, 1964, 2239, 3250, 3647
FriOI GRGCYC 2 cut(s) 403, 1084
Fsp4HI GCNGC 6 cut(s) 656, 1461, 1585, 3450, 3514, 3569
FspBI CTAG 9 cut(s) 395, 1266, 1722, 2003, 2096, 2241, 2448, 2633, 3110
GluI GCNGC 6 cut(s) 656, 1461, 1585, 3450, 3514, 3569
GsuI CTGGAG 1 cut(s) 3516
HaeIII GGCC 2 cut(s) 686, 1846
HapII CCGG 3 cut(s) 687, 1595, 1847
HincII GTYRAC 3 cut(s) 921, 2214, 2445
HindII GTYRAC 3 cut(s) 921, 2214, 2445
HindIII AAGCTT 2 cut(s) 2960, 3416
HpaI GTTAAC 1 cut(s) 2445
HpaII CCGG 3 cut(s) 687, 1595, 1847
HphI GGTGA 6 cut(s) 614, 1213, 2710, 2945, 2989, 3269
Hpy166II GTNNAC 8 cut(s) 895, 921, 944, 2214, 2445, 2688, 2786, 3361
Hpy8I GTNNAC 8 cut(s) 895, 921, 944, 2214, 2445, 2688, 2786, 3361
Hpy99I CGWCG 2 cut(s) 890, 3272
HpyAV CCTTC 9 cut(s) 350, 643, 850, 953, 2274, 2467, 2911, 2968, 3648
HpyCH4IV ACGT 8 cut(s) 126, 368, 415, 876, 1668, 1861, 1999, 3270
HpyF10VI GCNNNNNNNGC 7 cut(s) 407, 1266, 1409, 1843, 2037, 2408, 3415
HpySE526I ACGT 8 cut(s) 126, 368, 415, 876, 1668, 1861, 1999, 3270
KspAI GTTAAC 1 cut(s) 2445
Kzo9I GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
LguI GCTCTTC 1 cut(s) 1072
LmnI GCTCC 7 cut(s) 178, 265, 406, 415, 859, 2408, 3196
Lsp1109I GCAGC 6 cut(s) 667, 1447, 1571, 3436, 3500, 3580
LweI GCATC 9 cut(s) 149, 223, 595, 1533, 1726, 1755, 2066, 2467, 2902
MaeI CTAG 9 cut(s) 395, 1266, 1722, 2003, 2096, 2241, 2448, 2633, 3110
MaeII ACGT 8 cut(s) 126, 368, 415, 876, 1668, 1861, 1999, 3270
MaeIII GTNAC 7 cut(s) 55, 1315, 1953, 2120, 2698, 3229, 3550
MalI GATC 8 cut(s) 132, 714, 1062, 1121, 2025, 2490, 3300, 3529
MboI GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
MfeI CAATTG 3 cut(s) 429, 450, 2831
MflI RGATCY 5 cut(s) 130, 1119, 2023, 3298, 3527
MhlI GDGCHC 6 cut(s) 403, 792, 946, 1084, 2788, 3363
MlyI GAGTC 6 cut(s) 1670, 1715, 2253, 2644, 2957, 3342
MmeI TCCRAC 6 cut(s) 22, 892, 1689, 2211, 3291, 3306
Mph1103I ATGCAT 4 cut(s) 524, 1257, 1526, 1770
MroXI GAANNNNTTC 3 cut(s) 360, 1836, 2477
MslI CAYNNNNRTG 4 cut(s) 20, 1763, 3285, 3501
MspA1I CMGCKG 3 cut(s) 158, 1916, 1924
MspCI CTTAAG 2 cut(s) 2789, 3413
MspI CCGG 3 cut(s) 687, 1595, 1847
MspR9I CCNGG 3 cut(s) 1133, 1595, 2375
MunI CAATTG 3 cut(s) 429, 450, 2831
Mva1269I GAATGC 1 cut(s) 3007
MvaI CCWGG 2 cut(s) 1133, 2375
MvnI CGCG 1 cut(s) 3589
MwoI GCNNNNNNNGC 7 cut(s) 407, 1266, 1409, 1843, 2037, 2408, 3415
NciI CCSGG 1 cut(s) 1595
NcoI CCATGG 4 cut(s) 15, 75, 177, 2664
NdeI CATATG 2 cut(s) 349, 2543
NdeII GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
NheI GCTAGC 1 cut(s) 1265
NlaIV GGNNCC 6 cut(s) 132, 402, 2812, 2856, 3164, 3642
NmeAIII GCCGAG 1 cut(s) 3344
NmuCI GTSAC 3 cut(s) 55, 1315, 2698
NruI TCGCGA 1 cut(s) 3589
NsiI ATGCAT 4 cut(s) 524, 1257, 1526, 1770
NspI RCATGY 2 cut(s) 3449, 3575
PaeR7I CTCGAG 1 cut(s) 2756
PagI TCATGA 2 cut(s) 1968, 3286
PciSI GCTCTTC 1 cut(s) 1072
PcsI WCGNNNNNNNCGW 1 cut(s) 882
PctI GAATGC 1 cut(s) 3007
PdmI GAANNNNTTC 3 cut(s) 360, 1836, 2477
Pfl23II CGTACG 1 cut(s) 311
PfoI TCCNGGA 2 cut(s) 1131, 1593
PkrI GCNGC 6 cut(s) 657, 1462, 1586, 3451, 3515, 3570
PleI GAGTC 6 cut(s) 1669, 1714, 2252, 2644, 2956, 3341
PpsI GAGTC 6 cut(s) 1669, 1714, 2252, 2644, 2956, 3341
Ppu21I YACGTR 1 cut(s) 1862
PpuMI RGGWCCY 1 cut(s) 3185
PshBI ATTAAT 1 cut(s) 1854
PsiI TTATAA 1 cut(s) 1127
Psp1406I AACGTT 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 3185
Psp6I CCWGG 2 cut(s) 1131, 2373
PspEI GGTNACC 1 cut(s) 2698
PspGI CCWGG 2 cut(s) 1131, 2373
PspLI CGTACG 1 cut(s) 311
PspN4I GGNNCC 6 cut(s) 132, 402, 2812, 2856, 3164, 3642
PspPI GGNCC 2 cut(s) 2810, 3185
PspPPI RGGWCCY 1 cut(s) 3185
PsrI GAACNNNNNNTAC 2 cut(s) 2292, 2324
PstI CTGCAG 1 cut(s) 3518
PsuI RGATCY 5 cut(s) 130, 1119, 2023, 3298, 3527
PvuII CAGCTG 2 cut(s) 158, 1924
RruI TCGCGA 1 cut(s) 3589
RsaI GTAC 5 cut(s) 313, 771, 1087, 3439, 3659
RsaNI GTAC 5 cut(s) 312, 770, 1086, 3438, 3658
RseI CAYNNNNRTG 4 cut(s) 20, 1763, 3285, 3501
SapI GCTCTTC 1 cut(s) 1072
SatI GCNGC 6 cut(s) 656, 1461, 1585, 3450, 3514, 3569
Sau3AI GATC 8 cut(s) 130, 712, 1060, 1119, 2023, 2488, 3298, 3527
Sau96I GGNCC 2 cut(s) 2810, 3185
ScaI AGTACT 1 cut(s) 3439
SchI GAGTC 6 cut(s) 1670, 1715, 2253, 2644, 2957, 3342
ScrFI CCNGG 3 cut(s) 1133, 1595, 2375
SduI GDGCHC 6 cut(s) 403, 792, 946, 1084, 2788, 3363
SfaNI GCATC 9 cut(s) 149, 223, 595, 1533, 1726, 1755, 2066, 2467, 2902
SfcI CTRYAG 4 cut(s) 51, 2505, 2720, 3514
Sfr274I CTCGAG 1 cut(s) 2756
SinI GGWCC 2 cut(s) 2810, 3185
SlaI CTCGAG 1 cut(s) 2756
SmiMI CAYNNNNRTG 4 cut(s) 20, 1763, 3285, 3501
SmlI CTYRAG 3 cut(s) 2756, 2789, 3413
SmoI CTYRAG 3 cut(s) 2756, 2789, 3413
SsiI CCGC 3 cut(s) 580, 1914, 2217
SspI AATATT 3 cut(s) 1018, 2765, 3475
SspMI CTAG 9 cut(s) 395, 1266, 1722, 2003, 2096, 2241, 2448, 2633, 3110
StyD4I CCNGG 3 cut(s) 1131, 1593, 2373
StyI CCWWGG 7 cut(s) 15, 75, 177, 263, 1209, 2664, 2770
TaiI ACGT 8 cut(s) 129, 371, 418, 879, 1671, 1864, 2002, 3273
TaqI TCGA 4 cut(s) 112, 564, 2737, 2757
TatI WGTACW 1 cut(s) 3437
TseFI GTSAC 3 cut(s) 55, 1315, 2698
TseI GCWGC 6 cut(s) 655, 1460, 1584, 3449, 3513, 3568
Tsp45I GTSAC 3 cut(s) 55, 1315, 2698
TspGWI ACGGA 1 cut(s) 1334
Vha464I CTTAAG 2 cut(s) 2789, 3413
VneI GTGCAC 3 cut(s) 942, 2784, 3359
VpaK11BI GGWCC 2 cut(s) 2810, 3185
VspI ATTAAT 1 cut(s) 1854
XbaI TCTAGA 2 cut(s) 2002, 2095
XceI RCATGY 2 cut(s) 3449, 3575
XcmI CCANNNNNNNNNTGG 1 cut(s) 2821
XhoI CTCGAG 1 cut(s) 2756
XmiI GTMKAC 1 cut(s) 894
XmnI GAANNNNTTC 3 cut(s) 360, 1836, 2477
XspI CTAG 9 cut(s) 395, 1266, 1722, 2003, 2096, 2241, 2448, 2633, 3110
ZrmI AGTACT 1 cut(s) 3439
Zsp2I ATGCAT 4 cut(s) 524, 1257, 1526, 1770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.