RchiOBHm_Chr5g0080261

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015588
Physical Location & Seq
Reverse (-)
86150874 .. 86156845
5972 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35459

Sequence Viewer

Length: 3582 bp
ATGGAGCTCTATTCCGTCGAGATGGCGTTGGCGATAGCTGTGGGATTTGCGACAATGACACATTGGTTGGCTTCCATGGTTGAAAACCGTGAGCCAGTGTCTAATGGTAATCGAGAATTGGCCGACGTGGACTCCCCTGCATCTCTTCTTTCATCAGCTGAATCATCGTCAGCTTCCATGGTTGAAAACCCTGAGCCAGTACCGGAGGAAAATCAAGAATTGGTCCCCTCTACATCTCTTGTATCATCAGCAGCTCCTTGGCAGAAGTATGATGTTTTTCTGAGTTTCAGGGGTGCAGACACTCGTAAGGGTTTTGTATCCCACTTACACCATGAATTGCAGACTGTGAGAGGGATCCAAACATTCAAGGATGACAAAGATCTTGAAACAGGGGCCCCTATTTCTTCAAGTCTGCTAAAGGCAATCGAAGAATCAAATTTTGCAATTGTTGTTCTGTCGGAAAACTATGCTCAGTCTGCATGGTGTTTGGAGGAACTTACAAAGATTTGTCAATGCATGAAAGACAAGAACAGAATCCTACCCCTGTTTTATTATGTCCAACCAAGTGATGTGCGGCATCAAAATGGGACTTTTGGAGATGCTTTAACTAAGCATGGAAACTCGGGCCGGCACAATTCAGAGAAGGTGCAGCGGTGGAGGGATGCTTTACACAGAGTGGCCGGTTTCTCTGGGCGTAATGCACAAGATTTTCAGACTGAAAGAGATCTTGTCGAAGCCATTGTGGATGCTGTGGGCAGTAAAGTACGACCTATTGAAACTGAGTTTATTATTTCCTCGATCCATTTTGAACCATTTGAAGCAACAAGAGAAGCCATGGATAAAGTAATGGAGGCGCTAAAAGATGAAAACATCACTATCATTGGGGTTTACGGAATTGGAGGTGTTGGAAAGTCAACAATGGTGGAACATGTTGGTTTAGAAGCCCAAAAAAAAAGGCTTTTCCCTCAGGTGATTTTGGCTCGCATATCCCCAGAACCTAACGTGTGGAATATTCAAGGCACATTAGCAGACATGTTGGGTCTGAAACTGGTGGATGAGACAGTAATTGGAAGAGCCCGTACATTGAGGAAGAAGATAACAAGTGGAGATAGGATCCTTATAATCTTGGACAACATTTGGGAGGGAATAGAGTTGACAAGCATAGGAATTCCCAGATTCAACGAGCTTAAAAGATGCAATTCCAAGGTCCTACTGACAACAAGGAAAAGGGATGTTTGCTATCGTATGCAATGCCAAGCAAGCATTCGTCTCAATACCTTAACTGAACAAGATTCTTGGAAGTTGTTTGCAAAGGAAGCAAGAAATTCTTTTAAAACGTCGACTTTCTATAACAAAGCAAGGCAGGTATCAGGAGAATGTGGTGGTCTTCCAGTTGCGTTGAAAGCAGTTGCAAAGGCACTTGGGGATAAAGAGTTGGACGAATGGAACAAAGCTGCTGAGCGACTAAAGGTGTCTCAACCTCCCCACCCTGAAGACGAGAAAGTTGTGTTCGAATGCATTAAGTTAAGCTATGATTACTTGAAATCTGATGTTTCCAAGTCATGCTTCTTGCTTTGCTGCCTATTCCCGGAGGATTATGATATCAAAATTGAAGACTTGCTGAAGTATGGGATTGGGAAAGGATTGTTTGAAGAGTCCAACATGCAAAATGCCAGAGACACAATACATACAATCATCGAATCTCTTAAAGATTCCAGCTTGCTTTTGGATGCTAAAGGGATTAAGGGGTGCGTAAGGATGCATGATGTCATCAGAGATGTTGCCATACAAATTTCCTTACAATTTTTTGTAAGAGCTGGTTGTGAATTGCAGTATTGGCCAAGGATTGATGCAGGTAAAGGCTACATTGCGATCTCACTAATGAAGAACAAAATATGCACGCTTCCCGCTGAACACTTGGTATTTCCAAAACTTCATATTCTTTTGTTACAAAGTAATATTATGAATAACATCCCAGTTTCTTCATTTCAACGTCTAAAGGCATTAAGGGTCTTAGATCTTAGCTACACTGCCATTTCTTTACTACCCACATCATTCAATCTCCTAACCAGCCTCCACACTTTGTATCTAGATGGTTGCTTGTCAAGGATAAGTAACATATCCCTACTTGGACGGTTGAAAAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTTTGAAATTGCCTAAAGAAATAGGACAGTTGACCACACTAAGGATGCTGGACTTCACTAGTGTCTGTGTTGATATAGTTCCTTCTAAAGTGATAAGCAACCTCAATAAATTAGAGGAACTGTACATACAGTGCGATTTTGCGGACTGGGGGAGTAAAGCAGAGGGAAGTGAAGTCAAGGGAAATACAGTTGAGGAGAGCGAAGCTGAGGGAGCAAGAGAGGAAACTAATGCTTCCTTTGATGAGTTCACTAGCTTGTTACATCTACAGATTTTGAAGGTTTTCATATCTGATCCAAAATGCTTACCTACAGATGTTGAGGTAAAGTCTAATTGGAAAAGTTTTGACATATGTATCTGTAGAGACCCATCAATTAGAGAATCGCTTTTGGATCCAAGTTCTCCACTTGATTCAAAAGCCGATTCAAAAGCCTTGACTCTTGACACAACCATGGATACCTTACCAAAATGGTTTACTGATGTGGTAACCAAGAAAACAGAGAAGCTACAGTATAGAAACTGTCGGGGTTTAGTTAATGTTCTTAAGGAATATTACCAAGGGACCTTACATTCACTGAAGTATTTACATGTTATTGGTCCCAGTGACAAATTGGAACAGTTGATGACCACAATAACACCGATTCCAAATGAACCTGTGTTTGAGAATTTGGAAGAGTTGCATCTCGATGGAGTGAATTGTCTGAAGGAGTTATGTGTTGGTGAATTACCAAATCAGTCTCTATGCAAGCTTAGGTTACTGGAGGTGAGAGGTTGTCCTGAATTGATGAATGCACTTTTACAATCTAATTTGTTGAAGAGACTACCAAATTTGGAAAAACTAAGTTGTGAAGAGATGGATAAATTGGAGTATGTGTTTTCATATGAAGGGTCTGAGGTAGAACAAAGTACTCTGACCGAGTTGATAGAGATGAGACTGGAGAATCTTGGGAACCTAATAAAGATATGGAATGGTCCTGCTCCTTGTGAAATCTTTTGTCATGTTAAAACTTTGGTAGTTTTCGAGTGCAATAAGCTGAAAAATCTCTTTACATCTGACATAGCTCACCATCTTCATGACTTGGAAGATCTTTCAGTAGAGGCTTGCCTAGGGTTGGAGAGAGTTATTGAAGCAAGTGAGGAAACAGTGAATGACAAGATAGTTTTACCAAAATTGAAGAAGTTAGCTTTGAAAAAGCTTCTTAAGCTTACAAGGTTCTATGGTAGTACTGGAAGTGCTACTAATGATGAAGAATGTATTGAGTTTCCTTTATTGGAACACTCGCATGTGGAACGCTGCCATATTTTCAATTCTCCAGCTAAGGACATGTTGGTTTCAATGATGAATAAGCAGCCTGTTTTGATTCCTATGCAAGTGCAGAGAGAGGTCCGAGAGACACACCAACTTTAA

Protein Analysis

1193

Amino Acids

134.51

Weight (kDa)

5.7

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 89 - 259 1.6e-49 TIR domain
TIR_2 PF13676 92 - 185 3.9e-15 TIR domain
NB-ARC PF00931 277 - 440 9.8e-27 NB-ARC domain
WHD_DRP PF23559 528 - 595 3.6e-06 Disease resistance protein Winged helix domain
LRR_14 PF23598 628 - 775 1e-09 Leucine-rich repeat region
LRR_8 PF13855 643 - 700 3.2e-09 Leucine rich repeat
LRR_14 PF23598 695 - 769 3.1e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 948 - 1093 1.5e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1045 - 1160 1.9e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1121
Acc36I ACCTGC 2 cut(s) 1354, 1844
AccI GTMKAC 1 cut(s) 1340
AciI CCGC 4 cut(s) 574, 652, 1908, 2318
AclWI GGATC 8 cut(s) 349, 362, 793, 1108, 1121, 2462, 2561, 2574
AcoI YGGCCR 3 cut(s) 120, 678, 1838
AcsI RAATTY 6 cut(s) 436, 1167, 1324, 1791, 2839, 3001
AcuI CTGAAG 4 cut(s) 1512, 1643, 2771, 2897
AdeI CACNNNGTG 1 cut(s) 676
AfaI GTAC 6 cut(s) 201, 765, 1081, 2300, 3082, 3400
AfiI CCNNNNNNNGG 3 cut(s) 1588, 2217, 3286
AflII CTTAAG 2 cut(s) 2717, 3374
AflIII ACRYGT 5 cut(s) 928, 1002, 1032, 2761, 3498
AhlI ACTAGT 1 cut(s) 2234
AjiI CACGTC 1 cut(s) 127
AjuI GAANNNNNNNTTGG 4 cut(s) 1248, 1280, 1550, 1582
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 9 cut(s) 1052, 1274, 1479, 1671, 2532, 2916, 2986, 3100, 3560
AlwI GGATC 8 cut(s) 349, 362, 793, 1108, 1121, 2462, 2561, 2574
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 5 cut(s) 120, 393, 625, 678, 1838
ApaI GGGCCC 1 cut(s) 397
ApeKI GCWGC 6 cut(s) 251, 649, 1454, 1578, 3468, 3523
ApoI RAATTY 6 cut(s) 436, 1167, 1324, 1791, 2839, 3001
Asp700I GAANNNNTTC 1 cut(s) 2456
AspA2I CCTAGG 1 cut(s) 3280
AspLEI GCGC 1 cut(s) 856
AspS9I GGNCC 9 cut(s) 223, 393, 394, 625, 1207, 2736, 2771, 3146, 3559
AsuC2I CCSGG 1 cut(s) 1589
AsuHPI GGTGA 4 cut(s) 982, 2906, 2950, 3230
AsuII TTCGAA 1 cut(s) 1512
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 6 cut(s) 223, 1207, 2736, 2771, 3146, 3559
AvrII CCTAGG 1 cut(s) 3280
AxyI CCTNAGG 1 cut(s) 966
BaeGI GKGCMC 1 cut(s) 397
BaeI ACNNNNGTAYC 2 cut(s) 2512, 2545
BalI TGGCCA 1 cut(s) 1840
BamHI GGATCC 3 cut(s) 354, 1113, 2566
BanII GRGCYC 3 cut(s) 9, 397, 1078
BarI GAAGNNNNNNTAC 2 cut(s) 1063, 1095
BbsI GAAGAC 3 cut(s) 1379, 1500, 1620
Bbv12I GWGCWC 1 cut(s) 9
BbvCI CCTCAGC 1 cut(s) 2382
BbvI GCAGC 6 cut(s) 263, 661, 1441, 1565, 3455, 3535
BccI CCATC 6 cut(s) 16, 2087, 2551, 2855, 3022, 3249
BciVI GTATCC 2 cut(s) 328, 2623
BcnI CCSGG 1 cut(s) 1589
BcoDI GTCTC 9 cut(s) 1052, 1274, 1479, 1671, 2532, 2916, 2986, 3100, 3560
BcuI ACTAGT 1 cut(s) 2234
BfaI CTAG 4 cut(s) 2090, 2235, 2427, 3281
BfmI CTRYAG 4 cut(s) 2441, 2484, 2533, 2681
BfoI RGCGCY 1 cut(s) 857
BfrI CTTAAG 2 cut(s) 2717, 3374
BfuAI ACCTGC 2 cut(s) 1354, 1844
BfuI GTATCC 2 cut(s) 328, 2623
BglII AGATCT 4 cut(s) 379, 724, 2017, 3259
BisI GCNGC 7 cut(s) 252, 575, 650, 1455, 1579, 3469, 3524
BlnI CCTAGG 1 cut(s) 3280
BlpI GCTNAGC 1 cut(s) 1458
BlsI GCNGC 7 cut(s) 253, 576, 651, 1456, 1580, 3470, 3525
BmcAI AGTACT 2 cut(s) 3082, 3400
Bme1390I CCNGG 1 cut(s) 1589
Bme18I GGWCC 6 cut(s) 223, 1207, 2736, 2771, 3146, 3559
BmeT110I CYCGRG 1 cut(s) 622
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 9 cut(s) 223, 393, 394, 625, 1207, 2736, 2771, 3146, 3559
BmrFI CCNGG 1 cut(s) 1589
BmrI ACTGGG 3 cut(s) 1970, 2332, 2769
BmuI ACTGGG 3 cut(s) 1970, 2332, 2769
BpiI GAAGAC 3 cut(s) 1379, 1500, 1620
BpmI CTGGAG 3 cut(s) 2954, 3131, 3471
Bpu10I CCTNAGC 4 cut(s) 192, 2382, 2924, 3492
Bpu1102I GCTNAGC 1 cut(s) 1458
Bpu14I TTCGAA 1 cut(s) 1512
BpuEI CTTGAG 1 cut(s) 2168
BpuMI CCSGG 1 cut(s) 1589
BsaI GGTCTC 1 cut(s) 2532
BsaJI CCNNGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 3032, 3062
Bsc4I CCNNNNNNNGG 3 cut(s) 1588, 2217, 3286
Bse118I RCCGGY 2 cut(s) 627, 680
Bse21I CCTNAGG 1 cut(s) 966
Bse3DI GCAATG 2 cut(s) 1256, 1866
BseDI CCNNGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
BseGI GGATG 9 cut(s) 376, 667, 751, 1060, 1237, 1735, 1764, 1971, 2226
BseLI CCNNNNNNNGG 3 cut(s) 1588, 2217, 3286
BseMI GCAATG 2 cut(s) 1256, 1866
BseMII CTCAG 8 cut(s) 183, 272, 485, 771, 980, 1449, 2373, 3057
BseRI GAGGAG 1 cut(s) 2384
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 6 cut(s) 263, 661, 1441, 1565, 3455, 3535
BsgI GTGCAG 3 cut(s) 315, 668, 3569
BshFI GGCC 5 cut(s) 122, 395, 627, 680, 1840
BsiHKAI GWGCWC 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 4 cut(s) 203, 628, 681, 1589
BslFI GGGAC 4 cut(s) 209, 601, 2749, 2757
BslI CCNNNNNNNGG 3 cut(s) 1588, 2217, 3286
BsmAI GTCTC 9 cut(s) 1052, 1274, 1479, 1671, 2532, 2916, 2986, 3100, 3560
BsmBI CGTCTC 1 cut(s) 1274
BsmFI GGGAC 4 cut(s) 209, 601, 2749, 2757
BsmI GAATGC 3 cut(s) 1263, 1520, 2968
BsnI GGCC 5 cut(s) 122, 395, 627, 680, 1840
Bso31I GGTCTC 1 cut(s) 2532
BsoBI CYCGRG 1 cut(s) 622
Bsp119I TTCGAA 1 cut(s) 1512
Bsp120I GGGCCC 1 cut(s) 393
Bsp1286I GDGCHC 3 cut(s) 9, 397, 1078
Bsp1407I TGTACA 1 cut(s) 2298
Bsp1720I GCTNAGC 1 cut(s) 1458
Bsp19I CCATGG 4 cut(s) 75, 177, 834, 2625
BspACI CCGC 4 cut(s) 574, 652, 1908, 2318
BspANI GGCC 5 cut(s) 122, 395, 627, 680, 1840
BspCNI CTCAG 8 cut(s) 184, 273, 484, 772, 979, 1450, 2374, 3058
BspHI TCATGA 1 cut(s) 3247
BspMI ACCTGC 2 cut(s) 1354, 1844
BspPI GGATC 8 cut(s) 349, 362, 793, 1108, 1121, 2462, 2561, 2574
BspQI GCTCTTC 1 cut(s) 1066
BspT104I TTCGAA 1 cut(s) 1512
BspTI CTTAAG 2 cut(s) 2717, 3374
BspTNI GGTCTC 1 cut(s) 2532
BsrDI GCAATG 2 cut(s) 1256, 1866
BsrFI RCCGGY 2 cut(s) 627, 680
BsrGI TGTACA 1 cut(s) 2298
BssAI RCCGGY 2 cut(s) 627, 680
BssECI CCNNGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
BssT1I CCWWGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
Bst6I CTCTTC 6 cut(s) 150, 1066, 1647, 2841, 2984, 3018
BstAFI CTTAAG 2 cut(s) 2717, 3374
BstAUI TGTACA 1 cut(s) 2298
BstBI TTCGAA 1 cut(s) 1512
BstC8I GCNNGC 7 cut(s) 629, 982, 1261, 1721, 1901, 2921, 3277
BstDSI CCRYGG 4 cut(s) 75, 177, 834, 2625
BstEII GGTNACC 1 cut(s) 2659
BstF5I GGATG 9 cut(s) 376, 667, 751, 1060, 1237, 1735, 1764, 1971, 2226
BstH2I RGCGCY 1 cut(s) 857
BstHHI GCGC 1 cut(s) 856
BstMAI GTCTC 9 cut(s) 1052, 1274, 1479, 1671, 2532, 2916, 2986, 3100, 3560
BstMWI GCNNNNNNNGC 7 cut(s) 476, 1260, 1316, 1403, 1837, 2387, 3376
BstNSI RCATGY 6 cut(s) 932, 1036, 1666, 2765, 3461, 3502
BstPI GGTNACC 1 cut(s) 2659
BstSCI CCNGG 1 cut(s) 1587
BstSFI CTRYAG 4 cut(s) 2441, 2484, 2533, 2681
BstSLI GKGCMC 1 cut(s) 397
BstV1I GCAGC 6 cut(s) 263, 661, 1441, 1565, 3455, 3535
BstV2I GAAGAC 3 cut(s) 1379, 1500, 1620
BstX2I RGATCY 7 cut(s) 354, 379, 724, 1113, 2017, 2566, 3259
BstYI RGATCY 7 cut(s) 354, 379, 724, 1113, 2017, 2566, 3259
Bsu36I CCTNAGG 1 cut(s) 966
BsuI GTATCC 2 cut(s) 328, 2623
BsuRI GGCC 5 cut(s) 122, 395, 627, 680, 1840
BtgI CCRYGG 4 cut(s) 75, 177, 834, 2625
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 9 cut(s) 376, 667, 751, 1060, 1237, 1735, 1764, 1971, 2226
BtsI GCAGTG 1 cut(s) 2028
BtsIMutI CAGTG 6 cut(s) 102, 2028, 2312, 2747, 2782, 3324
BveI ACCTGC 2 cut(s) 1354, 1844
Cac8I GCNNGC 7 cut(s) 629, 982, 1261, 1721, 1901, 2921, 3277
CciI TCATGA 1 cut(s) 3247
CfoI GCGC 1 cut(s) 856
Cfr10I RCCGGY 2 cut(s) 627, 680
Cfr13I GGNCC 9 cut(s) 223, 393, 394, 625, 1207, 2736, 2771, 3146, 3559
Csp6I GTAC 6 cut(s) 200, 764, 1080, 2299, 3081, 3399
CspCI CAANNNNNGTGG 2 cut(s) 903, 938
CviQI GTAC 6 cut(s) 200, 764, 1080, 2299, 3081, 3399
DraI TTTAAA 1 cut(s) 1333
DraIII CACNNNGTG 1 cut(s) 676
EaeI YGGCCR 3 cut(s) 120, 678, 1838
Eam1104I CTCTTC 6 cut(s) 150, 1066, 1647, 2841, 2984, 3018
EarI CTCTTC 6 cut(s) 150, 1066, 1647, 2841, 2984, 3018
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
Eco24I GRGCYC 3 cut(s) 9, 397, 1078
Eco31I GGTCTC 1 cut(s) 2532
Eco32I GATATC 1 cut(s) 1603
Eco47I GGWCC 6 cut(s) 223, 1207, 2736, 2771, 3146, 3559
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 4 cut(s) 1512, 1643, 2771, 2897
Eco81I CCTNAGG 1 cut(s) 966
Eco88I CYCGRG 1 cut(s) 622
Eco91I GGTNACC 1 cut(s) 2659
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 4 cut(s) 393, 394, 1207, 2736
EcoO65I GGTNACC 1 cut(s) 2659
EcoRI GAATTC 1 cut(s) 1167
EcoRV GATATC 1 cut(s) 1603
EcoT14I CCWWGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
EcoT22I ATGCAT 3 cut(s) 518, 1520, 1764
EcoT38I GRGCYC 3 cut(s) 9, 397, 1078
ErhI CCWWGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
Esp3I CGTCTC 1 cut(s) 1274
FalI AAGNNNNNCTT 4 cut(s) 1312, 1344, 1550, 1582
FaqI GGGAC 4 cut(s) 209, 601, 2749, 2757
FauI CCCGC 1 cut(s) 1915
FauNDI CATATG 2 cut(s) 2525, 3055
FblI GTMKAC 1 cut(s) 1340
Fnu4HI GCNGC 7 cut(s) 252, 575, 650, 1455, 1579, 3469, 3524
FokI GGATG 9 cut(s) 383, 674, 758, 1067, 1244, 1742, 1771, 1958, 2233
FriOI GRGCYC 3 cut(s) 9, 397, 1078
Fsp4HI GCNGC 7 cut(s) 252, 575, 650, 1455, 1579, 3469, 3524
FspBI CTAG 4 cut(s) 2090, 2235, 2427, 3281
GlaI GCGC 1 cut(s) 855
GluI GCNGC 7 cut(s) 252, 575, 650, 1455, 1579, 3469, 3524
GsuI CTGGAG 3 cut(s) 2954, 3131, 3471
HaeII RGCGCY 1 cut(s) 857
HaeIII GGCC 5 cut(s) 122, 395, 627, 680, 1840
HapII CCGG 4 cut(s) 203, 628, 681, 1589
HhaI GCGC 1 cut(s) 856
Hin6I GCGC 1 cut(s) 854
HinP1I GCGC 1 cut(s) 854
HincII GTYRAC 4 cut(s) 915, 1155, 1341, 2208
HindII GTYRAC 4 cut(s) 915, 1155, 1341, 2208
HindIII AAGCTT 3 cut(s) 2921, 3368, 3377
HpaII CCGG 4 cut(s) 203, 628, 681, 1589
HphI GGTGA 4 cut(s) 982, 2906, 2950, 3230
Hpy166II GTNNAC 8 cut(s) 130, 889, 915, 1155, 1341, 2208, 2424, 2649
Hpy8I GTNNAC 8 cut(s) 130, 889, 915, 1155, 1341, 2208, 2424, 2649
Hpy99I CGWCG 3 cut(s) 20, 128, 1342
HpyAV CCTTC 5 cut(s) 637, 2268, 2446, 2872, 3053
HpyCH4IV ACGT 4 cut(s) 126, 1002, 1337, 1993
HpyF10VI GCNNNNNNNGC 7 cut(s) 476, 1260, 1316, 1403, 1837, 2387, 3376
HpySE526I ACGT 4 cut(s) 126, 1002, 1337, 1993
HspAI GCGC 1 cut(s) 854
KroI GCCGGC 1 cut(s) 627
KroNI GCCGGC 1 cut(s) 629
LguI GCTCTTC 1 cut(s) 1066
LmnI GCTCC 4 cut(s) 4, 259, 2387, 3157
Lsp1109I GCAGC 6 cut(s) 263, 661, 1441, 1565, 3455, 3535
MaeI CTAG 4 cut(s) 2090, 2235, 2427, 3281
MaeII ACGT 4 cut(s) 126, 1002, 1337, 1993
MaeIII GTNAC 6 cut(s) 1947, 2114, 2433, 2659, 2777, 2928
MfeI CAATTG 1 cut(s) 444
MflI RGATCY 7 cut(s) 354, 379, 724, 1113, 2017, 2566, 3259
MhlI GDGCHC 3 cut(s) 9, 397, 1078
MlsI TGGCCA 1 cut(s) 1840
MluNI TGGCCA 1 cut(s) 1840
MlyI GAGTC 3 cut(s) 125, 1664, 2605
MmeI TCCRAC 6 cut(s) 438, 583, 886, 1416, 1683, 3267
Mox20I TGGCCA 1 cut(s) 1840
Mph1103I ATGCAT 3 cut(s) 518, 1520, 1764
MroNI GCCGGC 1 cut(s) 627
MroXI GAANNNNTTC 1 cut(s) 2456
MscI TGGCCA 1 cut(s) 1840
MslI CAYNNNNRTG 5 cut(s) 582, 2624, 2859, 3246, 3456
Msp20I TGGCCA 1 cut(s) 1840
MspA1I CMGCKG 3 cut(s) 158, 652, 1910
MspCI CTTAAG 2 cut(s) 2717, 3374
MspI CCGG 4 cut(s) 203, 628, 681, 1589
MspR9I CCNGG 1 cut(s) 1589
MunI CAATTG 1 cut(s) 444
Mva1269I GAATGC 3 cut(s) 1263, 1520, 2968
MwoI GCNNNNNNNGC 7 cut(s) 476, 1260, 1316, 1403, 1837, 2387, 3376
NaeI GCCGGC 1 cut(s) 629
NciI CCSGG 1 cut(s) 1589
NcoI CCATGG 4 cut(s) 75, 177, 834, 2625
NdeI CATATG 2 cut(s) 2525, 3055
NgoMIV GCCGGC 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 2777
NsiI ATGCAT 3 cut(s) 518, 1520, 1764
NspI RCATGY 6 cut(s) 932, 1036, 1666, 2765, 3461, 3502
NspV TTCGAA 1 cut(s) 1512
PagI TCATGA 1 cut(s) 3247
PciI ACATGT 4 cut(s) 928, 1032, 2761, 3498
PciSI GCTCTTC 1 cut(s) 1066
PctI GAATGC 3 cut(s) 1263, 1520, 2968
PdiI GCCGGC 1 cut(s) 629
PdmI GAANNNNTTC 1 cut(s) 2456
PfoI TCCNGGA 1 cut(s) 1587
PkrI GCNGC 7 cut(s) 253, 576, 651, 1456, 1580, 3470, 3525
PleI GAGTC 3 cut(s) 125, 1663, 2605
PpsI GAGTC 3 cut(s) 125, 1663, 2605
PpuMI RGGWCCY 2 cut(s) 1207, 2736
PscI ACATGT 4 cut(s) 928, 1032, 2761, 3498
PsiI TTATAA 1 cut(s) 1121
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 2 cut(s) 1207, 2736
PspEI GGTNACC 1 cut(s) 2659
PspOMI GGGCCC 1 cut(s) 393
PspPI GGNCC 9 cut(s) 223, 393, 394, 625, 1207, 2736, 2771, 3146, 3559
PspPPI RGGWCCY 2 cut(s) 1207, 2736
PsrI GAACNNNNNNTAC 2 cut(s) 2286, 2318
PsuI RGATCY 7 cut(s) 354, 379, 724, 1113, 2017, 2566, 3259
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 6 cut(s) 201, 765, 1081, 2300, 3082, 3400
RsaNI GTAC 6 cut(s) 200, 764, 1080, 2299, 3081, 3399
RseI CAYNNNNRTG 5 cut(s) 582, 2624, 2859, 3246, 3456
SacI GAGCTC 1 cut(s) 9
SalI GTCGAC 1 cut(s) 1339
SapI GCTCTTC 1 cut(s) 1066
SatI GCNGC 7 cut(s) 252, 575, 650, 1455, 1579, 3469, 3524
Sau96I GGNCC 9 cut(s) 223, 393, 394, 625, 1207, 2736, 2771, 3146, 3559
ScaI AGTACT 2 cut(s) 3082, 3400
SchI GAGTC 3 cut(s) 125, 1664, 2605
ScrFI CCNGG 1 cut(s) 1589
SduI GDGCHC 3 cut(s) 9, 397, 1078
SfcI CTRYAG 4 cut(s) 2441, 2484, 2533, 2681
SfuI TTCGAA 1 cut(s) 1512
SinI GGWCC 6 cut(s) 223, 1207, 2736, 2771, 3146, 3559
SmiMI CAYNNNNRTG 5 cut(s) 582, 2624, 2859, 3246, 3456
SmlI CTYRAG 3 cut(s) 2147, 2717, 3374
SmoI CTYRAG 3 cut(s) 2147, 2717, 3374
SpeI ACTAGT 1 cut(s) 2234
SsiI CCGC 4 cut(s) 574, 652, 1908, 2318
SspI AATATT 3 cut(s) 1012, 1960, 2726
SspMI CTAG 4 cut(s) 2090, 2235, 2427, 3281
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 1587
StyI CCWWGG 9 cut(s) 75, 177, 257, 834, 1203, 1841, 2625, 2731, 3280
TaiI ACGT 4 cut(s) 129, 1005, 1340, 1996
TaqII GACCGA 1 cut(s) 3104
TatI WGTACW 3 cut(s) 2298, 3080, 3398
TauI GCSGC 1 cut(s) 577
TscAI CASTG 6 cut(s) 102, 2035, 2312, 2754, 2782, 3324
TseFI GTSAC 1 cut(s) 2777
TseI GCWGC 6 cut(s) 251, 649, 1454, 1578, 3468, 3523
Tsp45I GTSAC 1 cut(s) 2777
TspGWI ACGGA 2 cut(s) 4, 906
TspRI CASTG 6 cut(s) 102, 2035, 2312, 2754, 2782, 3324
Vha464I CTTAAG 2 cut(s) 2717, 3374
VpaK11BI GGWCC 6 cut(s) 223, 1207, 2736, 2771, 3146, 3559
XapI RAATTY 6 cut(s) 436, 1167, 1324, 1791, 2839, 3001
XbaI TCTAGA 1 cut(s) 2089
XceI RCATGY 6 cut(s) 932, 1036, 1666, 2765, 3461, 3502
XcmI CCANNNNNNNNNTGG 2 cut(s) 1723, 2782
XmaJI CCTAGG 1 cut(s) 3280
XmiI GTMKAC 1 cut(s) 1340
XmnI GAANNNNTTC 1 cut(s) 2456
XspI CTAG 4 cut(s) 2090, 2235, 2427, 3281
ZrmI AGTACT 2 cut(s) 3082, 3400
Zsp2I ATGCAT 3 cut(s) 518, 1520, 1764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.