Rw5G048450

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015588
Physical Location & Seq
Reverse (-)
84234269 .. 84238061
3793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048450.1

Sequence Viewer

Length: 3570 bp
ATGGCGTTGGCGATAGCTGTGGGATTTGCGACAATGACACATTGGTTGGCTTCCATGGTTGAAAACCGTGAGCCAGTGTCTAATGATAATCGAGAATTGGCCGACGTGGACCCCCCCTGCATCTCTTTTTTATCAGCTGAATCATCGTCAGCTTCCATGGTTGAAAACCCTGAGCCAATACAGGAGGAAAATCAAGAATTGGTCCCCTCTACATCTCTTGTATCATCAGCAGCTCCTTGGCAGAAGTATGATGTTTTTCTGAGTTTCAGGGGTGCGGACACTCGTAAGGGTTTTGTATCCCACTTACACCATGAATTGCAGACTGTGAGAGGGATCCAAACATTCAAGGATGACAAAGATCTTGAAACAGGGGCCCCTATTTCTTCAAGTCTGCTAAAGGCAATCGAAGAATCAAATTTTGCAATTGTTGTTCTGTCGGAAAACTATGCTCAGTCTGCATGGTGTTTGGAGGAACTTACAAAGATTTGTCAATGCATGAAAGACAAGAACAGAATCCTACCCCTGTTTTATTATGTCCAACCAAGTGATGTGCGGCATCAAAATGGGACTTTTGGAGATGCTTTAACTAAGCATGGAAACTCGGGCCGGCACAATTCAGAGAAGGTGCAGCGGTGGAGGGATGCTTTACACAGAGTGGCCGGTTTCTCTGGGCGTAATGCACAAGATTTTCAGACTGAAAGAGATCTTGTCGAAGCCATTGTGGATACTGTGGGCAGTAAAGTACGACCTATTGAAACTGAGTTTATTATTTCCTCGATCCATTTTGAACCATTTGAAGCAACGAGAGAAGCCATGGATAAAGTAATGGAGGCGCTAAAAGATGAAAACATCACTATCATTGGGGTTTACGGAATTGGAGGTGTTGGAAAGTCAACAATGGTGGAACATGTTGGTTTAGAAGCCCAAAAAAAAAGGCTTTTCCCTCAGGTGATTTTGGCTCGCATATCCCCAGAACCTAACGTGTGGAATATTCAAGGCACATTAGCAGACATGTTGGGTCTGAAACTGGTGGATGAGACAGTAATTGGAAGAGCCCGTACATTGAGGAAGAAGATAACAAGTGGAGATAGGATCCTTATAATCTTGGACAACATTTGGGAGGGAATAGAGTTGACAAGCATAGGAATTCCCAGATTCAACGAGCTTAAAAGATGCAATTCCAAGGTCCTACTGACAACAAGGAAAAGGGATGTTTGTTATCGTATGCAATGCCAAGCAAGCATTCGTCTCAATACCTTAACTGAACAAGATTCTTGGAAGTTGTTTGCAAAGGAAGCAAGAAATTCTTTTAAAACGTCGACTTTCTATAACAAAGCAAGGCAGGTATCAGGAGAATGTGGTGGTCTTCCAGTTGCGTTGAAAGCAGTTGCAAAGGCACTTGGGGATAAAGAGTTGGACGAATGGAACAAAGCCGCTGAGCGACTAAAGGTGTCTCAACCTCCCCACCCTGAAGACGAGAAAGTTGTGTTCGAATGCATTAAGTTAAGCTATGATTACTTGAAATCTGATGTTTCCAAGTCATGCTTCTTGCTTTGCTGCCTATTCCCGGAGGATTATGATATCAAAATTGAAGACTTGCTGAAGTATGGGATTGGGAAAGGATTGTTTGAAGAGTCCAACATGCAAAATGCCAGAGACACAATACATACAATCATCGAGTCTCTTAAAGATTCCAGCTTGCTTTTGGATGCTAAAGGGATTAAGGGGTGCGTAAGGATGCATGATGTCATCAGAGATGTTGCCATACAAATTTCCTTACAATTTTTTGTAAGAGCTGGTTGTGAATTGCAGTATTGGCCAAGGATTGATGCAGGTAAAGGCTACATTGCGATCTCACTAATGAAGAACAAAATATGCACGCTTCCCGCTGAACACTTGGTATTTCCAAAACTTCATATTCTTTTGTTACAAAGTAATATTATGAATAACATCCCAGTTTCTTCATTTCAACGTCTAAAGGCATTAAGGGTCTTAGATCTTAGCTACACTGCCATTTCTTTACTACCCACATCATTCAATCTCCTAACCAGCCTCCACACTTTGTATCTAGATGGTTGCTTGTCAAGGATAAGTAACATATCCCTACTTGGACAGTTGAAAAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTTTGAAATTGCCTAAAGAAATAGGACAGTTGACCACACTAAGGATGCTGGACTTCACTAGTGTCTGTGTTGATATAGTTCCTTCTAAAGTGATAAGCAACCTCAATAAATTAGAGGAACTGTACATACAGTGCGATTTTGCGGACTGGGGGAGTAAAGCAGAGGGAAGTGAAGTCGAGGGAAATACAGTTGAGGAGAGCGAAGCTGAGGGAGCAAGAGAGGAAACTAATGCTCCCTTTGATGAGTTCACTAGCTTGTTACATCTACAGATTTTGAAGGTTTTCATATCTGATCCAAAATGCTTACCTACAGATGTTGAGGTAAAGTCTAATTGGAAAAGTTTTGACATATGTATCTGTAGAGACCCATCAATTAGAGAATCGCTTTTGGATCCAAGTTCTCCACTTGATTCAAAAGCCGATTCAAAAGCCTTGACTCTTGACACAACCATGGATACCTTACCGCAATGGTTTACTGATGTGGTAACCAAGAAATCAGAGAAGCTACAGTATAGAAACTGTCGGGGTTTAGTTAATGTTCTTAAGGAATATTACCAAGGGACCTTACATTCACTGAAGTATTTACATGTTATTGGTCCCAGTGACAAATTGGAACAGTTGATGACCACAATAACACCGATTCCAAATGAACCTGTGTTTGAGAATTTGGAAGAGTTGCATCTCGATGGAGTGAATTGTCTGAAGGAGTTATGTGTTGGTGAATTACCAAATCAGTCTCTATGCAAGCTTAGGTTACTGGAGGTGAGAGGTTGTCCTGAATTGATGAATGCACTTTTACAATCTAATTTGTTGAAGAGACTACCAAATTTGGAAAAACTAAGTTGTGAAGAGATGGATAAATTGGAGTATGTGTTTTCATATGAAGGGTCTGAGGTAGAACAAAGTACTCTGACAGAGTTGATAGAGATGAGATTGGAGAATCTAGGGAACCTAATAAAGATATGGAATGGTCCTGCTCCTTGTGAAATCTTTTGTCATGTTAAAACTTTGGTAGTTTTCGAGTGCAATAAGCTGAAAAATCTCTTTACATCTGACATAGCTCACCATCTTCATGACTTGGAAGATCTTTCAGTAGAGGCTTGCCTAGGGTTGGAGAGAGTTATTGAAGCAAGTGAGGAAACAGTGAACGACAAGATAGTTTTACCAAAATTGAAGAAGTTAGCTTTGAAAAAGCTTCTTAAGCTTACAAGGTTCTATGGTAGTGCTGGAAGTGCTACTAATGATGAAGAATGTATTGAGTTTCCTTTATTGGAACACTCGCATGTGGAACGCTGCCATATTTTCAATTCTCCAGCTAAGGACATGTTGGTTTCAATGATGAATAAGCAGCCTGTTTTGATTCCTATGCAAGAGAGAGGTCCTAGAGACACACCAACTTTAAGCAGAAGAAGATGA

Protein Analysis

1189

Amino Acids

134.13

Weight (kDa)

5.68

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 82 - 251 2.7e-49 TIR domain
TIR_2 PF13676 85 - 178 3.8e-15 TIR domain
NB-ARC PF00931 270 - 433 9.8e-27 NB-ARC domain
WHD_DRP PF23559 521 - 588 3.6e-06 Disease resistance protein Winged helix domain
LRR_14 PF23598 621 - 768 3.8e-10 Leucine-rich repeat region
LRR_8 PF13855 636 - 693 3.2e-09 Leucine rich repeat
LRR_RPS2 PF23247 941 - 1086 1.9e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1038 - 1153 2.4e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1100
Acc36I ACCTGC 2 cut(s) 1333, 1823
AccI GTMKAC 1 cut(s) 1319
AciI CCGC 7 cut(s) 275, 553, 631, 1434, 1887, 2297, 2618
AclWI GGATC 8 cut(s) 328, 341, 772, 1087, 1100, 2441, 2540, 2553
AcoI YGGCCR 3 cut(s) 99, 657, 1817
AcsI RAATTY 6 cut(s) 415, 1146, 1303, 1770, 2818, 2980
AcuI CTGAAG 4 cut(s) 1491, 1622, 2750, 2876
AdeI CACNNNGTG 1 cut(s) 655
AfaI GTAC 4 cut(s) 744, 1060, 2279, 3061
AfiI CCNNNNNNNGG 3 cut(s) 1567, 2196, 3265
AflII CTTAAG 2 cut(s) 2696, 3353
AflIII ACRYGT 5 cut(s) 907, 981, 1011, 2740, 3477
AhlI ACTAGT 1 cut(s) 2213
AjiI CACGTC 1 cut(s) 106
AjuI GAANNNNNNNTTGG 4 cut(s) 1227, 1259, 1529, 1561
Alw26I GTCTC 9 cut(s) 1031, 1253, 1458, 1650, 1686, 2511, 2895, 2965, 3534
AlwI GGATC 8 cut(s) 328, 341, 772, 1087, 1100, 2441, 2540, 2553
Ama87I CYCGRG 1 cut(s) 601
AoxI GGCC 5 cut(s) 99, 372, 604, 657, 1817
ApaI GGGCCC 1 cut(s) 376
ApeKI GCWGC 5 cut(s) 230, 628, 1557, 3447, 3502
ApoI RAATTY 6 cut(s) 415, 1146, 1303, 1770, 2818, 2980
Asp700I GAANNNNTTC 1 cut(s) 2435
AspA2I CCTAGG 1 cut(s) 3259
AspLEI GCGC 1 cut(s) 835
AsuC2I CCSGG 1 cut(s) 1568
AsuHPI GGTGA 4 cut(s) 961, 2885, 2929, 3209
AsuII TTCGAA 1 cut(s) 1491
AvaI CYCGRG 1 cut(s) 601
AvaII GGWCC 7 cut(s) 109, 202, 1186, 2715, 2750, 3125, 3533
AvrII CCTAGG 1 cut(s) 3259
AxyI CCTNAGG 1 cut(s) 945
BaeGI GKGCMC 1 cut(s) 376
BaeI ACNNNNGTAYC 2 cut(s) 2491, 2524
BalI TGGCCA 1 cut(s) 1819
BamHI GGATCC 3 cut(s) 333, 1092, 2545
BanII GRGCYC 2 cut(s) 376, 1057
BarI GAAGNNNNNNTAC 2 cut(s) 1042, 1074
BbsI GAAGAC 3 cut(s) 1358, 1479, 1599
BbvCI CCTCAGC 1 cut(s) 2361
BbvI GCAGC 5 cut(s) 242, 640, 1544, 3434, 3514
BccI CCATC 5 cut(s) 2066, 2530, 2834, 3001, 3228
BciVI GTATCC 3 cut(s) 307, 718, 2602
BcnI CCSGG 1 cut(s) 1568
BcoDI GTCTC 9 cut(s) 1031, 1253, 1458, 1650, 1686, 2511, 2895, 2965, 3534
BcuI ACTAGT 1 cut(s) 2213
BfaI CTAG 6 cut(s) 2069, 2214, 2406, 3098, 3260, 3537
BfmI CTRYAG 4 cut(s) 2420, 2463, 2512, 2660
BfoI RGCGCY 1 cut(s) 836
BfrI CTTAAG 2 cut(s) 2696, 3353
BfuAI ACCTGC 2 cut(s) 1333, 1823
BfuI GTATCC 3 cut(s) 307, 718, 2602
BglII AGATCT 4 cut(s) 358, 703, 1996, 3238
BisI GCNGC 7 cut(s) 231, 554, 629, 1434, 1558, 3448, 3503
BlnI CCTAGG 1 cut(s) 3259
BlpI GCTNAGC 1 cut(s) 1437
BlsI GCNGC 7 cut(s) 232, 555, 630, 1435, 1559, 3449, 3504
BmcAI AGTACT 1 cut(s) 3061
Bme1390I CCNGG 1 cut(s) 1568
Bme18I GGWCC 7 cut(s) 109, 202, 1186, 2715, 2750, 3125, 3533
BmeT110I CYCGRG 1 cut(s) 601
BmgBI CACGTC 1 cut(s) 106
BmrFI CCNGG 1 cut(s) 1568
BmrI ACTGGG 3 cut(s) 1949, 2311, 2748
BmuI ACTGGG 3 cut(s) 1949, 2311, 2748
BpiI GAAGAC 3 cut(s) 1358, 1479, 1599
BpmI CTGGAG 2 cut(s) 2933, 3450
Bpu10I CCTNAGC 4 cut(s) 171, 2361, 2903, 3471
Bpu1102I GCTNAGC 1 cut(s) 1437
Bpu14I TTCGAA 1 cut(s) 1491
BpuEI CTTGAG 1 cut(s) 2147
BpuMI CCSGG 1 cut(s) 1568
BsaI GGTCTC 1 cut(s) 2511
BsaJI CCNNGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
BsaXI ACNNNNNCTCC 4 cut(s) 2371, 2401, 3011, 3041
Bsc4I CCNNNNNNNGG 3 cut(s) 1567, 2196, 3265
Bse118I RCCGGY 2 cut(s) 606, 659
Bse1I ACTGG 7 cut(s) 74, 1032, 1370, 1955, 2306, 2754, 2916
Bse21I CCTNAGG 1 cut(s) 945
Bse3DI GCAATG 3 cut(s) 1235, 1845, 2627
BseDI CCNNGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
BseGI GGATG 8 cut(s) 355, 646, 1039, 1216, 1714, 1743, 1950, 2205
BseLI CCNNNNNNNGG 3 cut(s) 1567, 2196, 3265
BseMI GCAATG 3 cut(s) 1235, 1845, 2627
BseMII CTCAG 8 cut(s) 162, 251, 464, 750, 959, 1428, 2352, 3036
BseNI ACTGG 7 cut(s) 74, 1032, 1370, 1955, 2306, 2754, 2916
BseRI GAGGAG 1 cut(s) 2363
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 5 cut(s) 242, 640, 1544, 3434, 3514
BsgI GTGCAG 1 cut(s) 647
BshFI GGCC 5 cut(s) 101, 374, 606, 659, 1819
BsiHKCI CYCGRG 1 cut(s) 601
BsiSI CCGG 3 cut(s) 607, 660, 1568
BslFI GGGAC 4 cut(s) 188, 580, 2728, 2736
BslI CCNNNNNNNGG 3 cut(s) 1567, 2196, 3265
BsmAI GTCTC 9 cut(s) 1031, 1253, 1458, 1650, 1686, 2511, 2895, 2965, 3534
BsmBI CGTCTC 1 cut(s) 1253
BsmFI GGGAC 4 cut(s) 188, 580, 2728, 2736
BsmI GAATGC 3 cut(s) 1242, 1499, 2947
BsnI GGCC 5 cut(s) 101, 374, 606, 659, 1819
Bso31I GGTCTC 1 cut(s) 2511
BsoBI CYCGRG 1 cut(s) 601
Bsp119I TTCGAA 1 cut(s) 1491
Bsp120I GGGCCC 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 376, 1057
Bsp1407I TGTACA 1 cut(s) 2277
Bsp1720I GCTNAGC 1 cut(s) 1437
Bsp19I CCATGG 4 cut(s) 54, 156, 813, 2604
BspACI CCGC 7 cut(s) 275, 553, 631, 1434, 1887, 2297, 2618
BspANI GGCC 5 cut(s) 101, 374, 606, 659, 1819
BspCNI CTCAG 8 cut(s) 163, 252, 463, 751, 958, 1429, 2353, 3037
BspHI TCATGA 1 cut(s) 3226
BspMI ACCTGC 2 cut(s) 1333, 1823
BspPI GGATC 8 cut(s) 328, 341, 772, 1087, 1100, 2441, 2540, 2553
BspQI GCTCTTC 1 cut(s) 1045
BspT104I TTCGAA 1 cut(s) 1491
BspTI CTTAAG 2 cut(s) 2696, 3353
BspTNI GGTCTC 1 cut(s) 2511
BsrDI GCAATG 3 cut(s) 1235, 1845, 2627
BsrFI RCCGGY 2 cut(s) 606, 659
BsrGI TGTACA 1 cut(s) 2277
BsrI ACTGG 7 cut(s) 74, 1032, 1370, 1955, 2306, 2754, 2916
BssAI RCCGGY 2 cut(s) 606, 659
BssECI CCNNGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
BssT1I CCWWGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
Bst6I CTCTTC 5 cut(s) 1045, 1626, 2820, 2963, 2997
BstAFI CTTAAG 2 cut(s) 2696, 3353
BstAUI TGTACA 1 cut(s) 2277
BstBI TTCGAA 1 cut(s) 1491
BstC8I GCNNGC 7 cut(s) 608, 961, 1240, 1700, 1880, 2900, 3256
BstDSI CCRYGG 4 cut(s) 54, 156, 813, 2604
BstEII GGTNACC 1 cut(s) 2638
BstF5I GGATG 8 cut(s) 355, 646, 1039, 1216, 1714, 1743, 1950, 2205
BstH2I RGCGCY 1 cut(s) 836
BstHHI GCGC 1 cut(s) 835
BstMAI GTCTC 9 cut(s) 1031, 1253, 1458, 1650, 1686, 2511, 2895, 2965, 3534
BstMWI GCNNNNNNNGC 8 cut(s) 455, 1239, 1295, 1382, 1816, 2366, 3355, 3386
BstNSI RCATGY 6 cut(s) 911, 1015, 1645, 2744, 3440, 3481
BstPI GGTNACC 1 cut(s) 2638
BstSCI CCNGG 1 cut(s) 1566
BstSFI CTRYAG 4 cut(s) 2420, 2463, 2512, 2660
BstSLI GKGCMC 1 cut(s) 376
BstV1I GCAGC 5 cut(s) 242, 640, 1544, 3434, 3514
BstV2I GAAGAC 3 cut(s) 1358, 1479, 1599
BstX2I RGATCY 7 cut(s) 333, 358, 703, 1092, 1996, 2545, 3238
BstYI RGATCY 7 cut(s) 333, 358, 703, 1092, 1996, 2545, 3238
Bsu36I CCTNAGG 1 cut(s) 945
BsuI GTATCC 3 cut(s) 307, 718, 2602
BsuRI GGCC 5 cut(s) 101, 374, 606, 659, 1819
BtgI CCRYGG 4 cut(s) 54, 156, 813, 2604
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 8 cut(s) 355, 646, 1039, 1216, 1714, 1743, 1950, 2205
BtsI GCAGTG 1 cut(s) 2007
BtsIMutI CAGTG 6 cut(s) 81, 2007, 2291, 2726, 2761, 3303
BveI ACCTGC 2 cut(s) 1333, 1823
Cac8I GCNNGC 7 cut(s) 608, 961, 1240, 1700, 1880, 2900, 3256
CciI TCATGA 1 cut(s) 3226
CfoI GCGC 1 cut(s) 835
Cfr10I RCCGGY 2 cut(s) 606, 659
Csp6I GTAC 4 cut(s) 743, 1059, 2278, 3060
CspCI CAANNNNNGTGG 2 cut(s) 882, 917
CviQI GTAC 4 cut(s) 743, 1059, 2278, 3060
DraI TTTAAA 1 cut(s) 1312
DraIII CACNNNGTG 1 cut(s) 655
EaeI YGGCCR 3 cut(s) 99, 657, 1817
Eam1104I CTCTTC 5 cut(s) 1045, 1626, 2820, 2963, 2997
EarI CTCTTC 5 cut(s) 1045, 1626, 2820, 2963, 2997
Eco130I CCWWGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
Eco24I GRGCYC 2 cut(s) 376, 1057
Eco31I GGTCTC 1 cut(s) 2511
Eco32I GATATC 1 cut(s) 1582
Eco47I GGWCC 7 cut(s) 109, 202, 1186, 2715, 2750, 3125, 3533
Eco57I CTGAAG 4 cut(s) 1491, 1622, 2750, 2876
Eco81I CCTNAGG 1 cut(s) 945
Eco88I CYCGRG 1 cut(s) 601
Eco91I GGTNACC 1 cut(s) 2638
EcoO109I RGGNCCY 5 cut(s) 372, 373, 1186, 2715, 3533
EcoO65I GGTNACC 1 cut(s) 2638
EcoRI GAATTC 1 cut(s) 1146
EcoRV GATATC 1 cut(s) 1582
EcoT14I CCWWGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
EcoT22I ATGCAT 3 cut(s) 497, 1499, 1743
EcoT38I GRGCYC 2 cut(s) 376, 1057
ErhI CCWWGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
Esp3I CGTCTC 1 cut(s) 1253
FalI AAGNNNNNCTT 4 cut(s) 1291, 1323, 1529, 1561
FaqI GGGAC 4 cut(s) 188, 580, 2728, 2736
FauI CCCGC 1 cut(s) 1894
FauNDI CATATG 2 cut(s) 2504, 3034
FblI GTMKAC 1 cut(s) 1319
Fnu4HI GCNGC 7 cut(s) 231, 554, 629, 1434, 1558, 3448, 3503
FokI GGATG 8 cut(s) 362, 653, 1046, 1223, 1721, 1750, 1937, 2212
FriOI GRGCYC 2 cut(s) 376, 1057
Fsp4HI GCNGC 7 cut(s) 231, 554, 629, 1434, 1558, 3448, 3503
FspBI CTAG 6 cut(s) 2069, 2214, 2406, 3098, 3260, 3537
GlaI GCGC 1 cut(s) 834
GluI GCNGC 7 cut(s) 231, 554, 629, 1434, 1558, 3448, 3503
GsuI CTGGAG 2 cut(s) 2933, 3450
HaeII RGCGCY 1 cut(s) 836
HaeIII GGCC 5 cut(s) 101, 374, 606, 659, 1819
HapII CCGG 3 cut(s) 607, 660, 1568
HhaI GCGC 1 cut(s) 835
Hin6I GCGC 1 cut(s) 833
HinP1I GCGC 1 cut(s) 833
HincII GTYRAC 4 cut(s) 894, 1134, 1320, 2187
HindII GTYRAC 4 cut(s) 894, 1134, 1320, 2187
HindIII AAGCTT 3 cut(s) 2900, 3347, 3356
HpaII CCGG 3 cut(s) 607, 660, 1568
HphI GGTGA 4 cut(s) 961, 2885, 2929, 3209
Hpy166II GTNNAC 9 cut(s) 109, 868, 894, 1134, 1320, 2187, 2403, 2628, 3301
Hpy188III TCNNGA 9 cut(s) 92, 194, 362, 1350, 2069, 2594, 2837, 2930, 3227
Hpy8I GTNNAC 9 cut(s) 109, 868, 894, 1134, 1320, 2187, 2403, 2628, 3301
Hpy99I CGWCG 2 cut(s) 107, 1321
HpyAV CCTTC 5 cut(s) 616, 2247, 2425, 2851, 3032
HpyCH4IV ACGT 4 cut(s) 105, 981, 1316, 1972
HpyF10VI GCNNNNNNNGC 8 cut(s) 455, 1239, 1295, 1382, 1816, 2366, 3355, 3386
HpySE526I ACGT 4 cut(s) 105, 981, 1316, 1972
HspAI GCGC 1 cut(s) 833
KroI GCCGGC 1 cut(s) 606
KroNI GCCGGC 1 cut(s) 608
LguI GCTCTTC 1 cut(s) 1045
LmnI GCTCC 4 cut(s) 238, 2366, 2392, 3136
Lsp1109I GCAGC 5 cut(s) 242, 640, 1544, 3434, 3514
MaeI CTAG 6 cut(s) 2069, 2214, 2406, 3098, 3260, 3537
MaeII ACGT 4 cut(s) 105, 981, 1316, 1972
MaeIII GTNAC 6 cut(s) 1926, 2093, 2412, 2638, 2756, 2907
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 7 cut(s) 333, 358, 703, 1092, 1996, 2545, 3238
MhlI GDGCHC 2 cut(s) 376, 1057
MlsI TGGCCA 1 cut(s) 1819
MluNI TGGCCA 1 cut(s) 1819
MlyI GAGTC 3 cut(s) 1643, 1688, 2584
MmeI TCCRAC 6 cut(s) 417, 562, 865, 1395, 1662, 3246
Mox20I TGGCCA 1 cut(s) 1819
Mph1103I ATGCAT 3 cut(s) 497, 1499, 1743
MroNI GCCGGC 1 cut(s) 606
MroXI GAANNNNTTC 1 cut(s) 2435
MscI TGGCCA 1 cut(s) 1819
MslI CAYNNNNRTG 5 cut(s) 561, 2603, 2838, 3225, 3435
Msp20I TGGCCA 1 cut(s) 1819
MspA1I CMGCKG 4 cut(s) 137, 631, 1436, 1889
MspCI CTTAAG 2 cut(s) 2696, 3353
MspI CCGG 3 cut(s) 607, 660, 1568
MspR9I CCNGG 1 cut(s) 1568
MunI CAATTG 1 cut(s) 423
Mva1269I GAATGC 3 cut(s) 1242, 1499, 2947
MwoI GCNNNNNNNGC 8 cut(s) 455, 1239, 1295, 1382, 1816, 2366, 3355, 3386
NaeI GCCGGC 1 cut(s) 608
NciI CCSGG 1 cut(s) 1568
NcoI CCATGG 4 cut(s) 54, 156, 813, 2604
NdeI CATATG 2 cut(s) 2504, 3034
NgoMIV GCCGGC 1 cut(s) 606
NmuCI GTSAC 1 cut(s) 2756
NsiI ATGCAT 3 cut(s) 497, 1499, 1743
NspI RCATGY 6 cut(s) 911, 1015, 1645, 2744, 3440, 3481
NspV TTCGAA 1 cut(s) 1491
PagI TCATGA 1 cut(s) 3226
PciI ACATGT 4 cut(s) 907, 1011, 2740, 3477
PciSI GCTCTTC 1 cut(s) 1045
PctI GAATGC 3 cut(s) 1242, 1499, 2947
PdiI GCCGGC 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 2435
PfoI TCCNGGA 1 cut(s) 1566
PkrI GCNGC 7 cut(s) 232, 555, 630, 1435, 1559, 3449, 3504
PleI GAGTC 3 cut(s) 1642, 1687, 2584
PpsI GAGTC 3 cut(s) 1642, 1687, 2584
PpuMI RGGWCCY 3 cut(s) 1186, 2715, 3533
PscI ACATGT 4 cut(s) 907, 1011, 2740, 3477
PsiI TTATAA 1 cut(s) 1100
Psp5II RGGWCCY 3 cut(s) 1186, 2715, 3533
PspEI GGTNACC 1 cut(s) 2638
PspOMI GGGCCC 1 cut(s) 372
PspPPI RGGWCCY 3 cut(s) 1186, 2715, 3533
PsrI GAACNNNNNNTAC 2 cut(s) 2265, 2297
PsuI RGATCY 7 cut(s) 333, 358, 703, 1092, 1996, 2545, 3238
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 4 cut(s) 744, 1060, 2279, 3061
RsaNI GTAC 4 cut(s) 743, 1059, 2278, 3060
RseI CAYNNNNRTG 5 cut(s) 561, 2603, 2838, 3225, 3435
SalI GTCGAC 1 cut(s) 1318
SapI GCTCTTC 1 cut(s) 1045
SatI GCNGC 7 cut(s) 231, 554, 629, 1434, 1558, 3448, 3503
ScaI AGTACT 1 cut(s) 3061
SchI GAGTC 3 cut(s) 1643, 1688, 2584
ScrFI CCNGG 1 cut(s) 1568
SduI GDGCHC 2 cut(s) 376, 1057
SfcI CTRYAG 4 cut(s) 2420, 2463, 2512, 2660
SfuI TTCGAA 1 cut(s) 1491
SinI GGWCC 7 cut(s) 109, 202, 1186, 2715, 2750, 3125, 3533
SmiMI CAYNNNNRTG 5 cut(s) 561, 2603, 2838, 3225, 3435
SmlI CTYRAG 3 cut(s) 2126, 2696, 3353
SmoI CTYRAG 3 cut(s) 2126, 2696, 3353
SpeI ACTAGT 1 cut(s) 2213
SsiI CCGC 7 cut(s) 275, 553, 631, 1434, 1887, 2297, 2618
SspI AATATT 3 cut(s) 991, 1939, 2705
SspMI CTAG 6 cut(s) 2069, 2214, 2406, 3098, 3260, 3537
StyD4I CCNGG 1 cut(s) 1566
StyI CCWWGG 9 cut(s) 54, 156, 236, 813, 1182, 1820, 2604, 2710, 3259
TaiI ACGT 4 cut(s) 108, 984, 1319, 1975
TatI WGTACW 2 cut(s) 2277, 3059
TauI GCSGC 2 cut(s) 556, 1436
TscAI CASTG 6 cut(s) 81, 2014, 2291, 2733, 2761, 3303
TseFI GTSAC 1 cut(s) 2756
TseI GCWGC 5 cut(s) 230, 628, 1557, 3447, 3502
Tsp45I GTSAC 1 cut(s) 2756
TspGWI ACGGA 1 cut(s) 885
TspRI CASTG 6 cut(s) 81, 2014, 2291, 2733, 2761, 3303
Vha464I CTTAAG 2 cut(s) 2696, 3353
VpaK11BI GGWCC 7 cut(s) 109, 202, 1186, 2715, 2750, 3125, 3533
XapI RAATTY 6 cut(s) 415, 1146, 1303, 1770, 2818, 2980
XbaI TCTAGA 1 cut(s) 2068
XceI RCATGY 6 cut(s) 911, 1015, 1645, 2744, 3440, 3481
XcmI CCANNNNNNNNNTGG 2 cut(s) 1702, 2761
XmaJI CCTAGG 1 cut(s) 3259
XmiI GTMKAC 1 cut(s) 1319
XmnI GAANNNNTTC 1 cut(s) 2435
XspI CTAG 6 cut(s) 2069, 2214, 2406, 3098, 3260, 3537
ZrmI AGTACT 1 cut(s) 3061
Zsp2I ATGCAT 3 cut(s) 497, 1499, 1743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.