Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0015588

Physical Location & Seq

Genomic Coordinates Forward (+)

3584309 .. 3589929

Gene Structure 5621 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00003050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGGACCTGGATCCCTCTACATCTCTTCCTTCATCAGCTCCTCTGAAGAAGTATGATGTTTTTCTGAGTTTCAGGGGTGCAGACACTCGTAAGGATTTTCTATCCCACTTACACCATGAATTGAGATATGTGAGAGGGCTTCAAACGTTCATGGATGACAAAGAGCTTGAACTAGGGGCTCCTATTTATCCAAGTCTGCTAAAGGCAATTGAAGAATCAAATTTTGCAATTGTTGTTTTGTCAAAAGACTATGCTCAATCTGCATGGTGTTTGGAGGAACTTACAAAGATTTGTGAATGCATGAAAGACAATAACAGAATTATACCGCTTTTTTATCATGTCGAACCTACTGATGTGCGGTATCAGAAGTGTAGTTTTCGAGATGCTTTCACAAATCATGTAAAAAATGGGTGGCACAGTTCAGAGCAGCTGCAGCGGTGGAAAAAAGCTTTAGAAACAGTAGCCAATTTCTCTGGGTGTAATGCGAAGGATTATAAGACTGAAAGAGAAGTTGTTGAAGCCATTGTGGAATCTGTGTGCGGGAGAGTAAGACCTATTGGAATTGAGTCTATTATTTCCTCAAGAGATTTTGGAGATTTTGAAGTATTTGAAGCAACAAGAGAAGCCATGGACAATGTAATGAAGGCGCTAGAAGATGAAAACGTCACTATCATTGGGGTCTACGGAATGCCAGGCATCGGAAAAACAACCATGGTGGAACATGTCGGTGTAGAAGCCCAAAAAAAGGGGCTATTCCGTGAGGTGACTCGGGCTGTGATATCCCAAAAGCCCAACTTGTGGAAGATTCAAGGAATATTAGCTTCCATGTTGGGTGTGACACTAAGGGGTGCAGACAAATTTGAAAGAGCCTGTGAATTGATGTCGATGATAAGGAGAAGAGAAAGGATCCTTATAATCCTGGATGACATTTGGACGAAAAGAGAGTTGTCAGACATAGGAATTCCTGGCCCCAAAGAGCTAAAAAGTCGCAATTCCAAGCTCCTACTCACCACAAGGAATAAGGATGTTTGCGATAGTATGCACTGCCAACCAAGAATTCCTATCGATATCTTGACTGAACAAGATTCTTGGAAGTTGTTTGAGAAGAATACAGGAAAATCTTTTGAACAAACAACCAAGTTCTATCGTGTTGCAAGGAAGGTAGCAAGAGAATGTGCCGGTCTTCCGCTTGCGTTGAAAGCAGTTGCAAAGGCACTTGGGAATAAAGATTTAGACCAATGGAGGGGAGCTTGTAAGCGACTAAAGGCGTCCAAAACTCCTTACCCGGAAGACAAGGGAGATGTATTCAAAAGCATAAAGCTAAGCTATGATTACCTAGAAACTAATGACTCCAAATCATGCTTCTTGCTTTGCTGCCTATTCCCAGAGGATTATGATATCCAAATTGAAGACCTGCTGAAGTATGGGATTGGGAAAGGATTGTTTCAGGAGTCTGACATGCAAGAAGCCAGAGCTGCAGTACATTCTGTGGTCCAGTCCCTTAAAGATTCTAACCTACTTTTGGATGGTAAAATGGACAGGGGATGTGTAAGGATGCATGATGTCATCAGAGATGTTGGCTTCTCAATTTCTTTATCTAAGGATGGACACAGGCAATTTTATGTAAGAGCTGGTTGGGAATTAGAGGATTGGCCAACGATTGATGCGCATGAATACTACACTGCAATCTCACTTATGAGGAACAAAATACCCGAGCTTCCCGAAGTCTTGGAATTTCCAGAACTCCAGATTCTCTTGTTACAAAGTAATTCTATGAATAAGATCCTAGATTCTTCTTTTAAACATCTAACGGCATTAAGGGTCTTAGATATTAGCGAGACTAGCATTTCTTTACTACCCTCATCATCCAATCTCCTAACCAGCCTCCACACTTTGTACCTAGATGGTTGCTTGTCAATGGAAAGTAACATATCCATACTTGGACAGTTGAAAGAACTTAAGATTCTTAGTATGAGAGAATTTCCTCGTGAGAAATTGCCAAAAGAAATAGGGGAGTTGACCAAACTAAGGATGCTGGACTTCACTAGTGAATCTGTTGGAATAGTTCCTTGTAACGTGATAGGCAACCTCAGTAAATTAGAAGAACTCTACGTGCAGTGCAACTTTGTGGACTGGGGGAGTAAAGTCGAGGGGAGTGAAGTCAAGGGAAGTATGGTTGAGGAGAGCAAAGCTGTGGGAGCAGGAGAGGAAACTACTGCTTTGTTTGATGAGTTAACTAGCTTGTTACATTTACGCATTTTGAAGGTTTTCATGTCTGATGCAAAATGCATGCATGTAAATGTTAGGCGTAAACTGAAATGGATAAACTTTGACATATGTATCAGCGGAGACCCGTCAATTAGAAAACAGCATTTGGATTCAAGTTCTTCACTTGACTATGCAAGAGCCTTGACTGTTGACATAACCATGGATACCTTACAGAAGTGGTTTATCGATGCTGTGACCAAGAAAACTGAGAAGCTGCAGTATATAAAGTGTCAGGATTTAGGTAATATCCTTATGGAATATGACGGGGGAGATTACATAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

LRR_8 NB-ARC TIR

Protein Analysis

850

Amino Acids

96.67

Weight (kDa)

6.04

Isoelectric Point (pI)

42.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	17 - 179	2.5e-45	TIR domain
TIR_2	PF13676	20 - 127	9.4e-16	TIR domain
NB-ARC	PF00931	208 - 372	2.2e-30	NB-ARC domain
LRR_8	PF13855	580 - 637	2.4e-07	Leucine rich repeat
LRR_14	PF23598	629 - 761	1.4e-11	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015588)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g44030 FvH4_3g44030
rosa_chinensis	RchiOBHm_Chr5g0079591 RchiOBHm_Chr5g0080151 RchiOBHm_Chr5g0080161 RchiOBHm_Chr5g0080261 RchiOBHm_Chr5g0080311
rosa_multiflora	Rmu_sc0001803.1_g000037 Rmu_sc0002513.1_g000013 Rmu_sc0007806.1_g000017 Rmu_sc0009793.1_g000003
rosa_roxburghii	Rroxscaffold_1G00003050
rosa_rugosa	Rorug05G0468000
rosa_wichuraiana	Rw0G023400 Rw5G048450 Rw5G048480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	495, 914
Acc16I	TGCGCA	1 cut(s)	1668
Acc36I	ACCTGC	1 cut(s)	1422
AccB7I	CCANNNNNTGG	1 cut(s)	798
AccI	GTMKAC	1 cut(s)	681
AciI	CCGC	6 cut(s)	326, 358, 436, 540, 1187, 2346
AclI	AACGTT	1 cut(s)	146
AclWI	GGATC	5 cut(s)	5, 18, 901, 914, 1777
AcoI	YGGCCR	1 cut(s)	1652
AcsI	RAATTY	6 cut(s)	220, 857, 960, 1056, 1733, 1979
AcuI	CTGAAG	2 cut(s)	65, 1439
AcyI	GRCGYC	1 cut(s)	1268
AfaI	GTAC	2 cut(s)	1482, 1898
AfiI	CCNNNNNNNGG	6 cut(s)	698, 745, 798, 1243, 1522, 2028
AflII	CTTAAG	1 cut(s)	1958
AflIII	ACRYGT	1 cut(s)	721
AhlI	ACTAGT	1 cut(s)	2045
AjnI	CCWGG	4 cut(s)	6, 691, 918, 964
AjuI	GAANNNNNNNTTGG	4 cut(s)	1041, 1073, 1346, 1378
Alw26I	GTCTC	2 cut(s)	1832, 2343
AlwI	GGATC	5 cut(s)	5, 18, 901, 914, 1777
Ama87I	CYCGRG	2 cut(s)	768, 1712
AoxI	GGCC	2 cut(s)	967, 1652
ApeKI	GCWGC	6 cut(s)	427, 430, 433, 1374, 1475, 2482
ApoI	RAATTY	6 cut(s)	220, 857, 960, 1056, 1733, 1979
Asp700I	GAANNNNTTC	1 cut(s)	2267
AspLEI	GCGC	2 cut(s)	649, 1669
AspS9I	GGNCC	3 cut(s)	4, 968, 1492
AsuC2I	CCSGG	1 cut(s)	1286
AsuHPI	GGTGA	2 cut(s)	775, 1000
AvaI	CYCGRG	2 cut(s)	768, 1712
AvaII	GGWCC	2 cut(s)	4, 1492
BaeI	ACNNNNGTAYC	2 cut(s)	2323, 2356
BalI	TGGCCA	1 cut(s)	1654
BamHI	GGATCC	2 cut(s)	10, 906
BanII	GRGCYC	1 cut(s)	181
BauI	CACGAG	1 cut(s)	1986
BbsI	GAAGAC	3 cut(s)	1175, 1296, 1416
BbvI	GCAGC	6 cut(s)	417, 439, 445, 1361, 1462, 2469
BccI	CCATC	3 cut(s)	1520, 1598, 1898
BceAI	ACGGC	1 cut(s)	1827
BciT130I	CCWGG	4 cut(s)	8, 693, 920, 966
BciVI	GTATCC	1 cut(s)	2425
BcnI	CCSGG	1 cut(s)	1286
BcoDI	GTCTC	2 cut(s)	1832, 2343
BcuI	ACTAGT	1 cut(s)	2045
BfaI	CTAG	8 cut(s)	173, 650, 1337, 1787, 1842, 1901, 2046, 2238
BfmI	CTRYAG	3 cut(s)	431, 1476, 2483
BfoI	RGCGCY	1 cut(s)	650
BfrI	CTTAAG	1 cut(s)	1958
BfuAI	ACCTGC	1 cut(s)	1422
BfuI	GTATCC	1 cut(s)	2425
BisI	GCNGC	6 cut(s)	428, 431, 434, 1375, 1476, 2483
BlpI	GCTNAGC	1 cut(s)	1322
BlsI	GCNGC	6 cut(s)	429, 432, 435, 1376, 1477, 2484
Bme1390I	CCNGG	5 cut(s)	8, 693, 920, 966, 1286
Bme18I	GGWCC	2 cut(s)	4, 1492
BmeT110I	CYCGRG	2 cut(s)	768, 1712
BmgT120I	GGNCC	3 cut(s)	4, 968, 1492
BmiI	GGNNCC	4 cut(s)	12, 180, 908, 970
BmrFI	CCNGG	5 cut(s)	8, 693, 920, 966, 1286
BmrI	ACTGGG	1 cut(s)	2143
BmsI	GCATC	7 cut(s)	373, 705, 1545, 1654, 2022, 2269, 2446
BmuI	ACTGGG	1 cut(s)	2143
BpiI	GAAGAC	3 cut(s)	1175, 1296, 1416
BpmI	CTGGAG	1 cut(s)	1730
Bpu1102I	GCTNAGC	1 cut(s)	1322
BpuEI	CTTGAG	1 cut(s)	565
BpuMI	CCSGG	1 cut(s)	1286
Bsa29I	ATCGAT	2 cut(s)	1065, 2454
BsaAI	YACGTR	1 cut(s)	2113
BsaHI	GRCGYC	1 cut(s)	1268
BsaI	GGTCTC	1 cut(s)	2343
BsaJI	CCNNGG	3 cut(s)	627, 711, 2427
Bsc4I	CCNNNNNNNGG	6 cut(s)	698, 745, 798, 1243, 1522, 2028
Bse118I	RCCGGY	1 cut(s)	1178
Bse1I	ACTGG	2 cut(s)	1495, 2138
BseBI	CCWGG	4 cut(s)	8, 693, 920, 966
BseCI	ATCGAT	2 cut(s)	1065, 2454
BseDI	CCNNGG	3 cut(s)	627, 711, 2427
BseGI	GGATG	9 cut(s)	160, 928, 1030, 1531, 1550, 1560, 1609, 1865, 2037
BseLI	CCNNNNNNNGG	6 cut(s)	698, 745, 798, 1243, 1522, 2028
BseMII	CTCAG	3 cut(s)	56, 2104, 2466
BseNI	ACTGG	2 cut(s)	1495, 2138
BseRI	GAGGAG	2 cut(s)	30, 2195
BseXI	GCAGC	6 cut(s)	417, 439, 445, 1361, 1462, 2469
BsgI	GTGCAG	3 cut(s)	99, 870, 2135
BshFI	GGCC	2 cut(s)	969, 1654
BshVI	ATCGAT	2 cut(s)	1065, 2454
BsiHKCI	CYCGRG	2 cut(s)	768, 1712
BsiSI	CCGG	2 cut(s)	1179, 1286
BslFI	GGGAC	1 cut(s)	1483
BslI	CCNNNNNNNGG	6 cut(s)	698, 745, 798, 1243, 1522, 2028
BsmAI	GTCTC	2 cut(s)	1832, 2343
BsmFI	GGGAC	1 cut(s)	1483
BsmI	GAATGC	2 cut(s)	302, 693
BsnI	GGCC	2 cut(s)	969, 1654
Bso31I	GGTCTC	1 cut(s)	2343
BsoBI	CYCGRG	2 cut(s)	768, 1712
Bsp1286I	GDGCHC	1 cut(s)	181
Bsp143I	GATC	3 cut(s)	10, 906, 1782
Bsp1720I	GCTNAGC	1 cut(s)	1322
Bsp19I	CCATGG	3 cut(s)	627, 711, 2427
BspACI	CCGC	6 cut(s)	326, 358, 436, 540, 1187, 2346
BspANI	GGCC	2 cut(s)	969, 1654
BspCNI	CTCAG	3 cut(s)	57, 2103, 2467
BspDI	ATCGAT	2 cut(s)	1065, 2454
BspLI	GGNNCC	4 cut(s)	12, 180, 908, 970
BspMAI	CTGCAG	3 cut(s)	435, 1480, 2487
BspMI	ACCTGC	1 cut(s)	1422
BspPI	GGATC	5 cut(s)	5, 18, 901, 914, 1777
BspTI	CTTAAG	1 cut(s)	1958
BspTNI	GGTCTC	1 cut(s)	2343
BsrFI	RCCGGY	1 cut(s)	1178
BsrI	ACTGG	2 cut(s)	1495, 2138
BssAI	RCCGGY	1 cut(s)	1178
BssECI	CCNNGG	3 cut(s)	627, 711, 2427
BssMI	GATC	3 cut(s)	10, 906, 1782
BssNI	GRCGYC	1 cut(s)	1268
BssSI	CACGAG	1 cut(s)	1986
BssT1I	CCWWGG	3 cut(s)	627, 711, 2427
Bst2BI	CACGAG	1 cut(s)	1986
Bst2UI	CCWGG	4 cut(s)	8, 693, 920, 966
Bst4CI	ACNGT	4 cut(s)	419, 460, 1947, 2416
Bst6I	CTCTTC	2 cut(s)	30, 892
BstACI	GRCGYC	1 cut(s)	1268
BstAFI	CTTAAG	1 cut(s)	1958
BstBAI	YACGTR	1 cut(s)	2113
BstC8I	GCNNGC	2 cut(s)	1191, 2291
BstDEI	CTNAG	9 cut(s)	65, 842, 1322, 1599, 1825, 1967, 2027, 2090, 2475
BstDSI	CCRYGG	3 cut(s)	627, 711, 2427
BstF5I	GGATG	9 cut(s)	160, 928, 1030, 1531, 1550, 1560, 1609, 1865, 2037
BstH2I	RGCGCY	1 cut(s)	650
BstHHI	GCGC	2 cut(s)	649, 1669
BstKTI	GATC	3 cut(s)	13, 909, 1785
BstMAI	GTCTC	2 cut(s)	1832, 2343
BstMBI	GATC	3 cut(s)	10, 906, 1782
BstMWI	GCNNNNNNNGC	6 cut(s)	260, 433, 1199, 1475, 1842, 2198
BstNI	CCWGG	4 cut(s)	8, 693, 920, 966
BstNSI	RCATGY	4 cut(s)	725, 1462, 2293, 2297
BstSCI	CCNGG	5 cut(s)	6, 691, 918, 964, 1284
BstSFI	CTRYAG	3 cut(s)	431, 1476, 2483
BstV1I	GCAGC	6 cut(s)	417, 439, 445, 1361, 1462, 2469
BstV2I	GAAGAC	3 cut(s)	1175, 1296, 1416
BstX2I	RGATCY	3 cut(s)	10, 906, 1782
BstYI	RGATCY	3 cut(s)	10, 906, 1782
Bsu15I	ATCGAT	2 cut(s)	1065, 2454
BsuI	GTATCC	1 cut(s)	2425
BsuRI	GGCC	2 cut(s)	969, 1654
BsuTUI	ATCGAT	2 cut(s)	1065, 2454
BtgI	CCRYGG	3 cut(s)	627, 711, 2427
BtsCI	GGATG	9 cut(s)	160, 928, 1030, 1531, 1550, 1560, 1609, 1865, 2037
BtsI	GCAGTG	3 cut(s)	1042, 1680, 2123
BtsIMutI	CAGTG	3 cut(s)	1042, 1680, 2123
BveI	ACCTGC	1 cut(s)	1422
Cac8I	GCNNGC	2 cut(s)	1191, 2291
CfoI	GCGC	2 cut(s)	649, 1669
Cfr10I	RCCGGY	1 cut(s)	1178
Cfr13I	GGNCC	3 cut(s)	4, 968, 1492
ClaI	ATCGAT	2 cut(s)	1065, 2454
CseI	GACGC	1 cut(s)	1257
Csp6I	GTAC	2 cut(s)	1481, 1897
CspCI	CAANNNNNGTGG	2 cut(s)	696, 731
CviQI	GTAC	2 cut(s)	1481, 1897
DdeI	CTNAG	9 cut(s)	65, 842, 1322, 1599, 1825, 1967, 2027, 2090, 2475
DpnI	GATC	3 cut(s)	12, 908, 1784
DpnII	GATC	3 cut(s)	10, 906, 1782
DraI	TTTAAA	1 cut(s)	1801
EaeI	YGGCCR	1 cut(s)	1652
Eam1104I	CTCTTC	2 cut(s)	30, 892
EarI	CTCTTC	2 cut(s)	30, 892
Eco130I	CCWWGG	3 cut(s)	627, 711, 2427
Eco24I	GRGCYC	1 cut(s)	181
Eco31I	GGTCTC	1 cut(s)	2343
Eco32I	GATATC	3 cut(s)	780, 1069, 1399
Eco47I	GGWCC	2 cut(s)	4, 1492
Eco57I	CTGAAG	2 cut(s)	65, 1439
Eco88I	CYCGRG	2 cut(s)	768, 1712
EcoRI	GAATTC	2 cut(s)	960, 1056
EcoRII	CCWGG	4 cut(s)	6, 691, 918, 964
EcoRV	GATATC	3 cut(s)	780, 1069, 1399
EcoT14I	CCWWGG	3 cut(s)	627, 711, 2427
EcoT22I	ATGCAT	4 cut(s)	302, 1560, 2291, 2295
EcoT38I	GRGCYC	1 cut(s)	181
ErhI	CCWWGG	3 cut(s)	627, 711, 2427
FalI	AAGNNNNNCTT	2 cut(s)	779, 811
FaqI	GGGAC	1 cut(s)	1483
FauI	CCCGC	1 cut(s)	533
FauNDI	CATATG	1 cut(s)	2336
FblI	GTMKAC	1 cut(s)	681
Fnu4HI	GCNGC	6 cut(s)	428, 431, 434, 1375, 1476, 2483
FokI	GGATG	9 cut(s)	167, 935, 1037, 1538, 1557, 1567, 1616, 1852, 2044
FriOI	GRGCYC	1 cut(s)	181
Fsp4HI	GCNGC	6 cut(s)	428, 431, 434, 1375, 1476, 2483
FspAI	RTGCGCAY	1 cut(s)	1668
FspBI	CTAG	8 cut(s)	173, 650, 1337, 1787, 1842, 1901, 2046, 2238
FspI	TGCGCA	1 cut(s)	1668
GlaI	GCGC	2 cut(s)	648, 1668
GluI	GCNGC	6 cut(s)	428, 431, 434, 1375, 1476, 2483
GsuI	CTGGAG	1 cut(s)	1730
HaeII	RGCGCY	1 cut(s)	650
HaeIII	GGCC	2 cut(s)	969, 1654
HapII	CCGG	2 cut(s)	1179, 1286
HgaI	GACGC	1 cut(s)	1257
HhaI	GCGC	2 cut(s)	649, 1669
Hin1I	GRCGYC	1 cut(s)	1268
Hin6I	GCGC	2 cut(s)	647, 1667
HinP1I	GCGC	2 cut(s)	647, 1667
HincII	GTYRAC	3 cut(s)	2019, 2235, 2419
HindII	GTYRAC	3 cut(s)	2019, 2235, 2419
HindIII	AAGCTT	1 cut(s)	447
HpaI	GTTAAC	1 cut(s)	2235
HpaII	CCGG	2 cut(s)	1179, 1286
HphI	GGTGA	2 cut(s)	775, 1000
Hpy166II	GTNNAC	6 cut(s)	682, 2019, 2131, 2235, 2312, 2419
Hpy188I	TCNGA	9 cut(s)	45, 66, 366, 424, 701, 952, 1456, 1571, 2278
Hpy188III	TCNNGA	9 cut(s)	380, 582, 1072, 1448, 1721, 1739, 1747, 1988, 2501
Hpy8I	GTNNAC	6 cut(s)	682, 2019, 2131, 2235, 2312, 2419
HpyAV	CCTTC	5 cut(s)	39, 481, 637, 1153, 2257
HpyCH4III	ACNGT	4 cut(s)	419, 460, 1947, 2416
HpyCH4IV	ACGT	4 cut(s)	146, 663, 2076, 2112
HpyF10VI	GCNNNNNNNGC	6 cut(s)	260, 433, 1199, 1475, 1842, 2198
HpyF3I	CTNAG	9 cut(s)	65, 842, 1322, 1599, 1825, 1967, 2027, 2090, 2475
HpySE526I	ACGT	4 cut(s)	146, 663, 2076, 2112
Hsp92I	GRCGYC	1 cut(s)	1268
HspAI	GCGC	2 cut(s)	647, 1667
KspAI	GTTAAC	1 cut(s)	2235
Kzo9I	GATC	3 cut(s)	10, 906, 1782
LmnI	GCTCC	5 cut(s)	43, 184, 1005, 1247, 2198
Lsp1109I	GCAGC	6 cut(s)	417, 439, 445, 1361, 1462, 2469
LweI	GCATC	7 cut(s)	373, 705, 1545, 1654, 2022, 2269, 2446
MaeI	CTAG	8 cut(s)	173, 650, 1337, 1787, 1842, 1901, 2046, 2238
MaeII	ACGT	4 cut(s)	146, 663, 2076, 2112
MaeIII	GTNAC	8 cut(s)	664, 763, 835, 1758, 1925, 2072, 2244, 2461
MalI	GATC	3 cut(s)	12, 908, 1784
MboI	GATC	3 cut(s)	10, 906, 1782
MfeI	CAATTG	2 cut(s)	207, 228
MflI	RGATCY	3 cut(s)	10, 906, 1782
MhlI	GDGCHC	1 cut(s)	181
MlsI	TGGCCA	1 cut(s)	1654
MluNI	TGGCCA	1 cut(s)	1654
MlyI	GAGTC	4 cut(s)	575, 760, 1343, 1460
MmeI	TCCRAC	1 cut(s)	2038
Mox20I	TGGCCA	1 cut(s)	1654
Mph1103I	ATGCAT	4 cut(s)	302, 1560, 2291, 2295
MroXI	GAANNNNTTC	1 cut(s)	2267
MscI	TGGCCA	1 cut(s)	1654
MseI	TTAA	5 cut(s)	1503, 1800, 1817, 1959, 2234
MslI	CAYNNNNRTG	3 cut(s)	726, 2298, 2426
Msp20I	TGGCCA	1 cut(s)	1654
MspA1I	CMGCKG	3 cut(s)	430, 436, 2346
MspCI	CTTAAG	1 cut(s)	1958
MspI	CCGG	2 cut(s)	1179, 1286
MspR9I	CCNGG	5 cut(s)	8, 693, 920, 966, 1286
MunI	CAATTG	2 cut(s)	207, 228
Mva1269I	GAATGC	2 cut(s)	302, 693
MvaI	CCWGG	4 cut(s)	8, 693, 920, 966
MwoI	GCNNNNNNNGC	6 cut(s)	260, 433, 1199, 1475, 1842, 2198
NciI	CCSGG	1 cut(s)	1286
NcoI	CCATGG	3 cut(s)	627, 711, 2427
NdeI	CATATG	1 cut(s)	2336
NdeII	GATC	3 cut(s)	10, 906, 1782
NlaIV	GGNNCC	4 cut(s)	12, 180, 908, 970
NmuCI	GTSAC	4 cut(s)	664, 763, 835, 2461
NsbI	TGCGCA	1 cut(s)	1668
NsiI	ATGCAT	4 cut(s)	302, 1560, 2291, 2295
NspI	RCATGY	4 cut(s)	725, 1462, 2293, 2297
PaeI	GCATGC	1 cut(s)	2293
PciI	ACATGT	1 cut(s)	721
PctI	GAATGC	2 cut(s)	302, 693
PdmI	GAANNNNTTC	1 cut(s)	2267
PflMI	CCANNNNNTGG	1 cut(s)	798
PfoI	TCCNGGA	1 cut(s)	918
PkrI	GCNGC	6 cut(s)	429, 432, 435, 1376, 1477, 2484
PleI	GAGTC	4 cut(s)	574, 760, 1343, 1459
PpsI	GAGTC	4 cut(s)	574, 760, 1343, 1459
Ppu21I	YACGTR	1 cut(s)	2113
PscI	ACATGT	1 cut(s)	721
PsiI	TTATAA	2 cut(s)	495, 914
Psp1406I	AACGTT	1 cut(s)	146
Psp6I	CCWGG	4 cut(s)	6, 691, 918, 964
PspGI	CCWGG	4 cut(s)	6, 691, 918, 964
PspN4I	GGNNCC	4 cut(s)	12, 180, 908, 970
PspPI	GGNCC	3 cut(s)	4, 968, 1492
PstI	CTGCAG	3 cut(s)	435, 1480, 2487
PsuI	RGATCY	3 cut(s)	10, 906, 1782
PvuII	CAGCTG	1 cut(s)	430
RsaI	GTAC	2 cut(s)	1482, 1898
RsaNI	GTAC	2 cut(s)	1481, 1897
RseI	CAYNNNNRTG	3 cut(s)	726, 2298, 2426
SaqAI	TTAA	5 cut(s)	1503, 1800, 1817, 1959, 2234
SatI	GCNGC	6 cut(s)	428, 431, 434, 1375, 1476, 2483
Sau3AI	GATC	3 cut(s)	10, 906, 1782
Sau96I	GGNCC	3 cut(s)	4, 968, 1492
SchI	GAGTC	4 cut(s)	575, 760, 1343, 1460
ScrFI	CCNGG	5 cut(s)	8, 693, 920, 966, 1286
SduI	GDGCHC	1 cut(s)	181
SfaNI	GCATC	7 cut(s)	373, 705, 1545, 1654, 2022, 2269, 2446
SfcI	CTRYAG	3 cut(s)	431, 1476, 2483
SinI	GGWCC	2 cut(s)	4, 1492
SmiMI	CAYNNNNRTG	3 cut(s)	726, 2298, 2426
SmlI	CTYRAG	2 cut(s)	580, 1958
SmoI	CTYRAG	2 cut(s)	580, 1958
SpeI	ACTAGT	1 cut(s)	2045
SphI	GCATGC	1 cut(s)	2293
SsiI	CCGC	6 cut(s)	326, 358, 436, 540, 1187, 2346
SspI	AATATT	1 cut(s)	816
SspMI	CTAG	8 cut(s)	173, 650, 1337, 1787, 1842, 1901, 2046, 2238
StyD4I	CCNGG	5 cut(s)	6, 691, 918, 964, 1284
StyI	CCWWGG	3 cut(s)	627, 711, 2427
TaaI	ACNGT	4 cut(s)	419, 460, 1947, 2416
TaiI	ACGT	4 cut(s)	149, 666, 2079, 2115
TaqI	TCGA	6 cut(s)	342, 379, 884, 1065, 2148, 2454
TatI	WGTACW	1 cut(s)	1480
Tru1I	TTAA	5 cut(s)	1503, 1800, 1817, 1959, 2234
Tru9I	TTAA	5 cut(s)	1503, 1800, 1817, 1959, 2234
TscAI	CASTG	3 cut(s)	1049, 1687, 2123
TseFI	GTSAC	4 cut(s)	664, 763, 835, 2461
TseI	GCWGC	6 cut(s)	427, 430, 433, 1374, 1475, 2482
Tsp45I	GTSAC	4 cut(s)	664, 763, 835, 2461
TspDTI	ATGAA	9 cut(s)	21, 132, 139, 317, 656, 672, 1686, 1790, 2260
TspGWI	ACGGA	2 cut(s)	699, 746
TspRI	CASTG	3 cut(s)	1049, 1687, 2123
Van91I	CCANNNNNTGG	1 cut(s)	798
Vha464I	CTTAAG	1 cut(s)	1958
VpaK11BI	GGWCC	2 cut(s)	4, 1492
XapI	RAATTY	6 cut(s)	220, 857, 960, 1056, 1733, 1979
XceI	RCATGY	4 cut(s)	725, 1462, 2293, 2297
XmiI	GTMKAC	1 cut(s)	681
XmnI	GAANNNNTTC	1 cut(s)	2267
XspI	CTAG	8 cut(s)	173, 650, 1337, 1787, 1842, 1901, 2046, 2238
Zsp2I	ATGCAT	4 cut(s)	302, 1560, 2291, 2295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.