RchiOBHm_Chr5g0080311

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015588
Physical Location & Seq
Reverse (-)
86246035 .. 86252340
6306 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35463

Sequence Viewer

Length: 3690 bp
ATGACCCGTACTGTGTCTTCTGGAGCATCTAATGCAATGTCAAAAATGGATGAGCCATTGAAGCGTGGTTCAAATGATATTGGATGCTATTTTGAAGAAGCAAAAGCTAAGAAGAAACAAAAAGCTAGACACGAAGAGGAAGTGAGGGAAGAAGTCAGAGTTATTGTACAAGAAGATGCAGAAATTGAAGGGCAAAAAACAAAGCAGCAAGATTCCATAGTGATTCAGCTGATGGAATCATCGTCAGCTCTCATGGTTGAAAACCCTGAGCCAAAATCAGAGGATCATCAAGAATCAATGGACCTGGATCCCTCTACATCTCTTCCTTCATCAGCTCCTCTGAAGAAGTATGATGTTTTTCTGAGTTTCAGGGGTGCAGACTCTCGTAAGGATTTTCTATCCCACTTACACCATGAATTGAGATATGTGAGAGGGCTTCAAACGTTCATGGATGACAAAGAGCTTGAACTAGGGGCTCCTATTTATCCAAGTCTGCTAAAGGCAATTGAAGAATCAAATTTTGCAATTGTTGTTTTGTCAAAAGACTATGCTCAATCTGCATGGTGTTTGGAGGAGCTTACAAAGATTTGTGAATGCATGAAAGACAATAACAGAATTATACCGCTTTTTTATCATGTCGAACCTACTGATGTGCGGTATCAGAAGTGTAGTTTTCGAGATGCTTTCACAAATCATGTAAAAAATGGGTGGCACAGTTCAGAGCAGCTGCAGCGGTGGAAAAAAGCTTTAGAAACAGTAGCCAATTTCTCTGGGTGTAATGCGAAGGATTATAAGACTGAAAGAGAAGTTGTTGAAGCCATTGTGGAATCTGTGTGCGGGAGAGTAAGACCTATTGGAATTGAGTCTATTATTTCCTCAAGAGATTTTGGAGATTTTGAAGTATTTGAAGCAACAAGAGAAGCCATGGACAATGTAATGAAGGCGCTAGAAGATGAAAACGTTACTATCATTGGGGTCTACGGAATGCCAGGCATCGGAAAAACAACCATGGTGGAACATGTCGGTGTAGAAGCCCAAAAAAAGGGGCTATTCCGTCAGGTGACTCGGGCTGTGATATCCCAAAAGCCCAACTTGTGGAAGATTCAAGGAAGATTAGCTTCCATGTTGGGTGTGACACTAAGGGGTGCAGACGAATTTGAAAGAGCCTGTGAATTGATGTCGATGATAAGGAGAAGAGAAAGGATCCTTATAATCCTGGATGACATTTGGACGAAAAGAGAGTTGTCAGACATAGGAATTCCTGGCCCCAAAGAGCTAAAAAGTCGCAATTCCAAGCTCCTACTCACCACAAGGAATAAGGATGTTTGCGATAGTATGCACTGCCAACCAAGAATTCCTATTGATATCTTGACTGAACAAGATTCTTGGAAGTTGTTTGAGAAGAATACAGGAAAATCTTTTGAACAAACAACCAAGTTCTATCGTGTTGCAAGGAAGGTAGCAAGAGAATGTGCCGGTCTTCCGCTTGCGTTGAAAGCAGTTGCAAAGGCACTTGGGAATAAAGATTTAGACCAATGGAGGGGAGCTTGTAAGCGACTAAAGGCGTCCAAAACTCCTTACCCGGAAGACAAGGGAGATGTATTCAAAAGCATAAAGCTAAGCTATGATTACCTAGAAACTAATGACTCCAAATCATGCTTCTTGCTTTGCTGCCTATTCCCAGAGGATTATGATATCCAAATTGAAGACCTGCTGAAGTATGGGATTGGGAAAGGATTGTTTCAGGAGTCTGACATGCAAGAAGCCAGAGCCGCAGTACATTCTGTGGTCCAGTCCCTTAAAGATTCTAACCTACTTTTGGATGGTAAAATGGACAGGGGATGCGTAAGGATGCATGATGTCATCAGAGATGTTGGCTTCTCAATTTCTTTATCTAAGGATGGACACAGGCAATTTTATGTAAGAGCTGGTTGGGAATTAGAGGATTGGCCAACGATTGATGCGCATGAATACTACACTGCAATCTCACTTATGAGGAACAAAATACCCGAGCTTCCCGAAGTCTTGGAATTTCCAGAACTCCAGATTCTCTTGTTACAAAGTAATTCTATGAATAAGATCCTAGATTCTTCTTTTAAACATCTAACGGCATTAAGGGTCTTAGATATTAGCGAGACTAGCATTTCTTTACTACCCTCATCATCCAATCTCCTAACCAGCCTCCACACTTTGTACCTAGATGGTTGCTTGTCAATGGAAAGTAACATATCCATACTTGGACAGTTGAAAGAACTTAAGATTCTTAGTATGAGAGAATTTCCTCGTGAGAAATTGCCAAAAGAAATAGGAGAGTTGACCAAACTAAGGATGCTGGACTTCACCAGTGAATCTGTTGGAATAGTTCCTTGTAACGTGATAGGCAACCTCAGTAAATTAGAAGAACTCTACATGCAGTGCAACTTTGTGGACTGGGGGAGTAAAGTCGAGGGGAGTGAAGTCAAGGGAAGTGTGGTTGAGGAGAGCAAAGCTGTGGGAGCAGGAGAGGAAACTACTGCTTTGTTTGATGAGTTAACTAGCTTGTTACATTTACGCATTTTGAAGGTTTTCATGTCTGATGCAAAATGCATGCATGTAAATGTTAGGCGTAAACTGAAATGGATAAACTTTGACATATGTATCAGCGGAGACCCATCAATTAGAAAACAGCATTTGGATTCAAGTTCTTCACTTGACTATGCAAGAGCCTTGACTGTTGACATAACCATGGATACCTTACAGAAGTGGTTTATCGATGCTGTGACCAAGAGAACTGAGAAGCTGCAGTATATAAAGTGTCAGGATTTAGGTAATATCCTTATGGAATATGACTGGGGGAGATTACATAAACTGAAGCATTTATCTGTTATCGGGCCCAATGAGAAATTGAAAGAGTTGATGAACACAACAATATGGATTCAAAATGAACCTGTGTTTCAGAATTTGGAAGAGTTGCATCTCGTTGAAGTGAATTGTCTGAAGGAGTTATGTGTTGGTGAATTACCAAATGAATCTCTATGCAAGCTTAGGTTACTGAAGGTGACAGGTTGTCATAAATTGATGAATGCACTTTTACAATCTAATTTGTTGCAGAGACTACAAAATCTGGAAATACTAATTTGTGAAGAGATGGATGTATTGGAGTATGTGTTTGCATGTGAAGTGTTTGACGCAGAACAAATTACTCTGAGAAAATTGACAGAGATGAGATTGGAGAACCTAGTGAACCTGATAAAGATATGGAATGGTCCTGCTCCACATGCAGTCTTTCATCATGTTAAAATTTTGGTAGTTTCTGGGTGCTTTAAGCTGAAATATCTCTTCACTTCAGAAGTAGCTCAATGTCTTCATGAATTGGAAGATCTTAGGGTAGAGGTTTGCCAAAGGTTGGAGAGGGTTATTGAAGCAAGTGAGGAAACAGTCAACAACAAGATAGTTTTTCCAAAATTGAAGATCTTAGCTTTGAAACAGCTTCCGAAGCTTACAAGGTTCTATGGTAGTACTGGAAGTGGTACTAATGATGAAGAAAATATTGAGTTTCCTTTATTGGAACACTCGCATGTGGAACGCTGCAATAGTTTCAAATCTCCAGCTAAGGACATGTTGGTTTCAGTGATGAAAAAGCAGCTTGTTTTGATTCCTATGCAAGAGTCAGATCGGAGAGAGACACCTACTTTAAGCAGAAGAAGATGA

Protein Analysis

1229

Amino Acids

140.38

Weight (kDa)

6.08

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 116 - 278 6.9e-45 TIR domain
TIR_2 PF13676 119 - 226 1.5e-15 TIR domain
NB-ARC PF00931 307 - 471 5.7e-31 NB-ARC domain
LRR_8 PF13855 679 - 736 3.8e-07 Leucine rich repeat
LRR_14 PF23598 728 - 987 4.4e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 980 - 1113 8.4e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1078 - 1195 8e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 792, 1211
Acc16I TGCGCA 1 cut(s) 1965
Acc36I ACCTGC 1 cut(s) 1719
AccB7I CCANNNNNTGG 2 cut(s) 1095, 3385
AccI GTMKAC 1 cut(s) 978
AciI CCGC 7 cut(s) 623, 655, 733, 837, 1484, 1773, 2643
AclI AACGTT 2 cut(s) 443, 960
AclWI GGATC 6 cut(s) 291, 302, 315, 1198, 1211, 2074
AcoI YGGCCR 1 cut(s) 1949
AcsI RAATTY 8 cut(s) 517, 1154, 1257, 1353, 2030, 2276, 2938, 3279
AcuI CTGAAG 6 cut(s) 362, 1736, 2870, 2996, 3053, 3309
AcyI GRCGYC 1 cut(s) 1565
AfaI GTAC 6 cut(s) 10, 168, 1779, 2195, 3499, 3511
AfiI CCNNNNNNNGG 7 cut(s) 995, 1042, 1095, 1540, 1819, 2325, 3385
AflII CTTAAG 1 cut(s) 2255
AflIII ACRYGT 2 cut(s) 1018, 3597
AhdI GACNNNNNGTC 1 cut(s) 3045
AjnI CCWGG 4 cut(s) 303, 988, 1215, 1261
AjuI GAANNNNNNNTTGG 4 cut(s) 1338, 1370, 1643, 1675
Alw26I GTCTC 4 cut(s) 2129, 2640, 3085, 3656
AlwI GGATC 6 cut(s) 291, 302, 315, 1198, 1211, 2074
Ama87I CYCGRG 2 cut(s) 1065, 2009
AoxI GGCC 3 cut(s) 1264, 1949, 2870
ApaI GGGCCC 1 cut(s) 2874
ApeKI GCWGC 8 cut(s) 205, 724, 727, 730, 1671, 2779, 3567, 3622
ApoI RAATTY 8 cut(s) 517, 1154, 1257, 1353, 2030, 2276, 2938, 3279
Asp700I GAANNNNTTC 1 cut(s) 2564
AspLEI GCGC 2 cut(s) 946, 1966
AspS9I GGNCC 6 cut(s) 301, 1265, 1789, 2870, 2871, 3245
AsuC2I CCSGG 1 cut(s) 1583
AsuHPI GGTGA 5 cut(s) 1072, 1297, 2332, 3005, 3049
AvaI CYCGRG 2 cut(s) 1065, 2009
AvaII GGWCC 3 cut(s) 301, 1789, 3245
BaeGI GKGCMC 1 cut(s) 2874
BaeI ACNNNNGTAYC 2 cut(s) 2620, 2653
BalI TGGCCA 1 cut(s) 1951
BamHI GGATCC 2 cut(s) 307, 1203
BanII GRGCYC 2 cut(s) 478, 2874
BarI GAAGNNNNNNTAC 1 cut(s) 33
BauI CACGAG 1 cut(s) 2283
BbsI GAAGAC 5 cut(s) 9, 1472, 1593, 1713, 3335
BbvI GCAGC 8 cut(s) 217, 714, 736, 742, 1658, 2766, 3554, 3634
BccI CCATC 6 cut(s) 226, 1817, 1895, 2195, 2659, 3121
BceAI ACGGC 1 cut(s) 2124
BciT130I CCWGG 4 cut(s) 305, 990, 1217, 1263
BciVI GTATCC 1 cut(s) 2722
BcnI CCSGG 1 cut(s) 1583
BcoDI GTCTC 4 cut(s) 2129, 2640, 3085, 3656
BfaI CTAG 9 cut(s) 126, 470, 947, 1634, 2084, 2139, 2198, 2535, 3218
BfmI CTRYAG 2 cut(s) 728, 2780
BfoI RGCGCY 1 cut(s) 947
BfrI CTTAAG 1 cut(s) 2255
BfuAI ACCTGC 1 cut(s) 1719
BfuI GTATCC 1 cut(s) 2722
BglII AGATCT 2 cut(s) 3358, 3450
BisI GCNGC 9 cut(s) 206, 725, 728, 731, 1672, 1773, 2780, 3568, 3623
BlpI GCTNAGC 1 cut(s) 1619
BlsI GCNGC 9 cut(s) 207, 726, 729, 732, 1673, 1774, 2781, 3569, 3624
BmcAI AGTACT 1 cut(s) 3499
Bme1390I CCNGG 5 cut(s) 305, 990, 1217, 1263, 1583
Bme18I GGWCC 3 cut(s) 301, 1789, 3245
BmeRI GACNNNNNGTC 1 cut(s) 3045
BmeT110I CYCGRG 2 cut(s) 1065, 2009
BmgT120I GGNCC 6 cut(s) 301, 1265, 1789, 2870, 2871, 3245
BmiI GGNNCC 5 cut(s) 309, 477, 1205, 1267, 2872
BmrFI CCNGG 5 cut(s) 305, 990, 1217, 1263, 1583
BmrI ACTGGG 2 cut(s) 2440, 2839
BmuI ACTGGG 2 cut(s) 2440, 2839
BpiI GAAGAC 5 cut(s) 9, 1472, 1593, 1713, 3335
BpmI CTGGAG 3 cut(s) 42, 2027, 3570
Bpu10I CCTNAGC 3 cut(s) 267, 3023, 3591
Bpu1102I GCTNAGC 1 cut(s) 1619
BpuEI CTTGAG 1 cut(s) 862
BpuMI CCSGG 1 cut(s) 1583
Bsa29I ATCGAT 1 cut(s) 2751
BsaHI GRCGYC 1 cut(s) 1565
BsaI GGTCTC 1 cut(s) 2640
BsaJI CCNNGG 3 cut(s) 924, 1008, 2724
BsaXI ACNNNNNCTCC 2 cut(s) 3131, 3161
Bsc4I CCNNNNNNNGG 7 cut(s) 995, 1042, 1095, 1540, 1819, 2325, 3385
Bse118I RCCGGY 1 cut(s) 1475
Bse1I ACTGG 5 cut(s) 1792, 2343, 2435, 2834, 3505
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 4 cut(s) 305, 990, 1217, 1263
BseCI ATCGAT 1 cut(s) 2751
BseDI CCNNGG 3 cut(s) 924, 1008, 2724
BseLI CCNNNNNNNGG 7 cut(s) 995, 1042, 1095, 1540, 1819, 2325, 3385
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 5 cut(s) 258, 353, 2401, 2763, 3176
BseNI ACTGG 5 cut(s) 1792, 2343, 2435, 2834, 3505
BseRI GAGGAG 3 cut(s) 327, 587, 2492
BseSI GKGCMC 1 cut(s) 2874
BseXI GCAGC 8 cut(s) 217, 714, 736, 742, 1658, 2766, 3554, 3634
BsgI GTGCAG 2 cut(s) 396, 1167
BshFI GGCC 3 cut(s) 1266, 1951, 2872
BshVI ATCGAT 1 cut(s) 2751
BsiHKCI CYCGRG 2 cut(s) 1065, 2009
BsiSI CCGG 2 cut(s) 1476, 1583
BslFI GGGAC 1 cut(s) 1780
BslI CCNNNNNNNGG 7 cut(s) 995, 1042, 1095, 1540, 1819, 2325, 3385
BsmAI GTCTC 4 cut(s) 2129, 2640, 3085, 3656
BsmFI GGGAC 1 cut(s) 1780
BsmI GAATGC 3 cut(s) 599, 990, 3067
BsnI GGCC 3 cut(s) 1266, 1951, 2872
Bso31I GGTCTC 1 cut(s) 2640
BsoBI CYCGRG 2 cut(s) 1065, 2009
Bsp120I GGGCCC 1 cut(s) 2870
Bsp1286I GDGCHC 2 cut(s) 478, 2874
Bsp1407I TGTACA 1 cut(s) 166
Bsp143I GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
Bsp1720I GCTNAGC 1 cut(s) 1619
Bsp19I CCATGG 3 cut(s) 924, 1008, 2724
BspACI CCGC 7 cut(s) 623, 655, 733, 837, 1484, 1773, 2643
BspANI GGCC 3 cut(s) 1266, 1951, 2872
BspCNI CTCAG 5 cut(s) 259, 354, 2400, 2764, 3177
BspDI ATCGAT 1 cut(s) 2751
BspHI TCATGA 1 cut(s) 3346
BspLI GGNNCC 5 cut(s) 309, 477, 1205, 1267, 2872
BspMAI CTGCAG 2 cut(s) 732, 2784
BspMI ACCTGC 1 cut(s) 1719
BspPI GGATC 6 cut(s) 291, 302, 315, 1198, 1211, 2074
BspTI CTTAAG 1 cut(s) 2255
BspTNI GGTCTC 1 cut(s) 2640
BsrDI GCAATG 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 1475
BsrGI TGTACA 1 cut(s) 166
BsrI ACTGG 5 cut(s) 1792, 2343, 2435, 2834, 3505
BssAI RCCGGY 1 cut(s) 1475
BssECI CCNNGG 3 cut(s) 924, 1008, 2724
BssMI GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
BssNI GRCGYC 1 cut(s) 1565
BssSI CACGAG 1 cut(s) 2283
BssT1I CCWWGG 3 cut(s) 924, 1008, 2724
Bst2BI CACGAG 1 cut(s) 2283
Bst2UI CCWGG 4 cut(s) 305, 990, 1217, 1263
Bst4CI ACNGT 6 cut(s) 13, 716, 757, 2244, 2713, 3418
Bst6I CTCTTC 6 cut(s) 129, 327, 1189, 2940, 3117, 3323
BstACI GRCGYC 1 cut(s) 1565
BstAFI CTTAAG 1 cut(s) 2255
BstAPI GCANNNNNTGC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 166
BstC8I GCNNGC 3 cut(s) 1488, 2588, 3020
BstDSI CCRYGG 3 cut(s) 924, 1008, 2724
BstH2I RGCGCY 1 cut(s) 947
BstHHI GCGC 2 cut(s) 946, 1966
BstKTI GATC 7 cut(s) 286, 310, 1206, 2082, 3361, 3453, 3655
BstMAI GTCTC 4 cut(s) 2129, 2640, 3085, 3656
BstMBI GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
BstNI CCWGG 4 cut(s) 305, 990, 1217, 1263
BstNSI RCATGY 9 cut(s) 1022, 1759, 2413, 2590, 2594, 3156, 3260, 3560, 3601
BstSCI CCNGG 5 cut(s) 303, 988, 1215, 1261, 1581
BstSFI CTRYAG 2 cut(s) 728, 2780
BstSLI GKGCMC 1 cut(s) 2874
BstV1I GCAGC 8 cut(s) 217, 714, 736, 742, 1658, 2766, 3554, 3634
BstV2I GAAGAC 5 cut(s) 9, 1472, 1593, 1713, 3335
BstX2I RGATCY 5 cut(s) 307, 1203, 2079, 3358, 3450
BstYI RGATCY 5 cut(s) 307, 1203, 2079, 3358, 3450
Bsu15I ATCGAT 1 cut(s) 2751
BsuI GTATCC 1 cut(s) 2722
BsuRI GGCC 3 cut(s) 1266, 1951, 2872
BsuTUI ATCGAT 1 cut(s) 2751
BtgI CCRYGG 3 cut(s) 924, 1008, 2724
BtsI GCAGTG 3 cut(s) 1339, 1977, 2420
BtsIMutI CAGTG 5 cut(s) 1339, 1977, 2350, 2420, 3615
BveI ACCTGC 1 cut(s) 1719
Cac8I GCNNGC 3 cut(s) 1488, 2588, 3020
CciI TCATGA 1 cut(s) 3346
CfoI GCGC 2 cut(s) 946, 1966
Cfr10I RCCGGY 1 cut(s) 1475
Cfr13I GGNCC 6 cut(s) 301, 1265, 1789, 2870, 2871, 3245
ClaI ATCGAT 1 cut(s) 2751
CseI GACGC 2 cut(s) 1554, 3176
Csp6I GTAC 6 cut(s) 9, 167, 1778, 2194, 3498, 3510
CspCI CAANNNNNGTGG 2 cut(s) 993, 1028
CviQI GTAC 6 cut(s) 9, 167, 1778, 2194, 3498, 3510
DpnI GATC 7 cut(s) 285, 309, 1205, 2081, 3360, 3452, 3654
DpnII GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
DraI TTTAAA 1 cut(s) 2098
DriI GACNNNNNGTC 1 cut(s) 3045
EaeI YGGCCR 1 cut(s) 1949
Eam1104I CTCTTC 6 cut(s) 129, 327, 1189, 2940, 3117, 3323
Eam1105I GACNNNNNGTC 1 cut(s) 3045
EarI CTCTTC 6 cut(s) 129, 327, 1189, 2940, 3117, 3323
Eco130I CCWWGG 3 cut(s) 924, 1008, 2724
Eco24I GRGCYC 2 cut(s) 478, 2874
Eco31I GGTCTC 1 cut(s) 2640
Eco32I GATATC 3 cut(s) 1077, 1366, 1696
Eco47I GGWCC 3 cut(s) 301, 1789, 3245
Eco57I CTGAAG 6 cut(s) 362, 1736, 2870, 2996, 3053, 3309
Eco88I CYCGRG 2 cut(s) 1065, 2009
EcoRI GAATTC 2 cut(s) 1257, 1353
EcoRII CCWGG 4 cut(s) 303, 988, 1215, 1261
EcoRV GATATC 3 cut(s) 1077, 1366, 1696
EcoT14I CCWWGG 3 cut(s) 924, 1008, 2724
EcoT22I ATGCAT 4 cut(s) 599, 1857, 2588, 2592
EcoT38I GRGCYC 2 cut(s) 478, 2874
ErhI CCWWGG 3 cut(s) 924, 1008, 2724
FalI AAGNNNNNCTT 4 cut(s) 1076, 1108, 1102, 1134
FaqI GGGAC 1 cut(s) 1780
FauI CCCGC 1 cut(s) 830
FauNDI CATATG 1 cut(s) 2633
FblI GTMKAC 1 cut(s) 978
Fnu4HI GCNGC 9 cut(s) 206, 725, 728, 731, 1672, 1773, 2780, 3568, 3623
FriOI GRGCYC 2 cut(s) 478, 2874
Fsp4HI GCNGC 9 cut(s) 206, 725, 728, 731, 1672, 1773, 2780, 3568, 3623
FspAI RTGCGCAY 1 cut(s) 1965
FspBI CTAG 9 cut(s) 126, 470, 947, 1634, 2084, 2139, 2198, 2535, 3218
FspI TGCGCA 1 cut(s) 1965
GlaI GCGC 2 cut(s) 945, 1965
GluI GCNGC 9 cut(s) 206, 725, 728, 731, 1672, 1773, 2780, 3568, 3623
GsuI CTGGAG 3 cut(s) 42, 2027, 3570
HaeII RGCGCY 1 cut(s) 947
HaeIII GGCC 3 cut(s) 1266, 1951, 2872
HapII CCGG 2 cut(s) 1476, 1583
HgaI GACGC 2 cut(s) 1554, 3176
HhaI GCGC 2 cut(s) 946, 1966
Hin1I GRCGYC 1 cut(s) 1565
Hin6I GCGC 2 cut(s) 944, 1964
HinP1I GCGC 2 cut(s) 944, 1964
HincII GTYRAC 4 cut(s) 2316, 2532, 2716, 3421
HindII GTYRAC 4 cut(s) 2316, 2532, 2716, 3421
HindIII AAGCTT 3 cut(s) 744, 3020, 3476
HpaI GTTAAC 1 cut(s) 2532
HpaII CCGG 2 cut(s) 1476, 1583
HphI GGTGA 5 cut(s) 1072, 1297, 2332, 3005, 3049
Hpy166II GTNNAC 8 cut(s) 979, 2316, 2428, 2532, 2609, 2716, 3223, 3421
Hpy8I GTNNAC 8 cut(s) 979, 2316, 2428, 2532, 2609, 2716, 3223, 3421
HpyAV CCTTC 8 cut(s) 182, 336, 778, 934, 1450, 2554, 2971, 3028
HpyCH4III ACNGT 6 cut(s) 13, 716, 757, 2244, 2713, 3418
HpyCH4IV ACGT 3 cut(s) 443, 960, 2373
HpySE526I ACGT 3 cut(s) 443, 960, 2373
Hsp92I GRCGYC 1 cut(s) 1565
HspAI GCGC 2 cut(s) 944, 1964
KspAI GTTAAC 1 cut(s) 2532
Kzo9I GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
LmnI GCTCC 8 cut(s) 23, 340, 481, 574, 1302, 1544, 2495, 3256
Lsp1109I GCAGC 8 cut(s) 217, 714, 736, 742, 1658, 2766, 3554, 3634
MaeI CTAG 9 cut(s) 126, 470, 947, 1634, 2084, 2139, 2198, 2535, 3218
MaeII ACGT 3 cut(s) 443, 960, 2373
MalI GATC 7 cut(s) 285, 309, 1205, 2081, 3360, 3452, 3654
MboI GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
MfeI CAATTG 2 cut(s) 504, 525
MflI RGATCY 5 cut(s) 307, 1203, 2079, 3358, 3450
MhlI GDGCHC 2 cut(s) 478, 2874
MlsI TGGCCA 1 cut(s) 1951
MluNI TGGCCA 1 cut(s) 1951
MlyI GAGTC 6 cut(s) 374, 872, 1057, 1640, 1757, 3656
MmeI TCCRAC 2 cut(s) 2335, 3366
Mox20I TGGCCA 1 cut(s) 1951
Mph1103I ATGCAT 4 cut(s) 599, 1857, 2588, 2592
MroXI GAANNNNTTC 1 cut(s) 2564
MscI TGGCCA 1 cut(s) 1951
MseI TTAA 8 cut(s) 1800, 2097, 2114, 2256, 2531, 3276, 3303, 3674
MslI CAYNNNNRTG 4 cut(s) 1023, 2595, 2723, 3555
Msp20I TGGCCA 1 cut(s) 1951
MspA1I CMGCKG 4 cut(s) 229, 727, 733, 2643
MspCI CTTAAG 1 cut(s) 2255
MspI CCGG 2 cut(s) 1476, 1583
MspR9I CCNGG 5 cut(s) 305, 990, 1217, 1263, 1583
MunI CAATTG 2 cut(s) 504, 525
Mva1269I GAATGC 3 cut(s) 599, 990, 3067
MvaI CCWGG 4 cut(s) 305, 990, 1217, 1263
NciI CCSGG 1 cut(s) 1583
NcoI CCATGG 3 cut(s) 924, 1008, 2724
NdeI CATATG 1 cut(s) 2633
NdeII GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
NlaIV GGNNCC 5 cut(s) 309, 477, 1205, 1267, 2872
NmuCI GTSAC 4 cut(s) 1060, 1132, 2758, 3037
NsbI TGCGCA 1 cut(s) 1965
NsiI ATGCAT 4 cut(s) 599, 1857, 2588, 2592
NspI RCATGY 9 cut(s) 1022, 1759, 2413, 2590, 2594, 3156, 3260, 3560, 3601
PaeI GCATGC 1 cut(s) 2590
PagI TCATGA 1 cut(s) 3346
PciI ACATGT 2 cut(s) 1018, 3597
PctI GAATGC 3 cut(s) 599, 990, 3067
PdmI GAANNNNTTC 1 cut(s) 2564
PflMI CCANNNNNTGG 2 cut(s) 1095, 3385
PfoI TCCNGGA 1 cut(s) 1215
PkrI GCNGC 9 cut(s) 207, 726, 729, 732, 1673, 1774, 2781, 3569, 3624
PleI GAGTC 6 cut(s) 374, 871, 1057, 1640, 1756, 3655
PpsI GAGTC 6 cut(s) 374, 871, 1057, 1640, 1756, 3655
PscI ACATGT 2 cut(s) 1018, 3597
PsiI TTATAA 2 cut(s) 792, 1211
Psp1406I AACGTT 2 cut(s) 443, 960
Psp6I CCWGG 4 cut(s) 303, 988, 1215, 1261
PspGI CCWGG 4 cut(s) 303, 988, 1215, 1261
PspN4I GGNNCC 5 cut(s) 309, 477, 1205, 1267, 2872
PspOMI GGGCCC 1 cut(s) 2870
PspPI GGNCC 6 cut(s) 301, 1265, 1789, 2870, 2871, 3245
PstI CTGCAG 2 cut(s) 732, 2784
PsuI RGATCY 5 cut(s) 307, 1203, 2079, 3358, 3450
PvuII CAGCTG 2 cut(s) 229, 727
RsaI GTAC 6 cut(s) 10, 168, 1779, 2195, 3499, 3511
RsaNI GTAC 6 cut(s) 9, 167, 1778, 2194, 3498, 3510
RseI CAYNNNNRTG 4 cut(s) 1023, 2595, 2723, 3555
SaqAI TTAA 8 cut(s) 1800, 2097, 2114, 2256, 2531, 3276, 3303, 3674
SatI GCNGC 9 cut(s) 206, 725, 728, 731, 1672, 1773, 2780, 3568, 3623
Sau3AI GATC 7 cut(s) 283, 307, 1203, 2079, 3358, 3450, 3652
Sau96I GGNCC 6 cut(s) 301, 1265, 1789, 2870, 2871, 3245
ScaI AGTACT 1 cut(s) 3499
SchI GAGTC 6 cut(s) 374, 872, 1057, 1640, 1757, 3656
ScrFI CCNGG 5 cut(s) 305, 990, 1217, 1263, 1583
SduI GDGCHC 2 cut(s) 478, 2874
SfcI CTRYAG 2 cut(s) 728, 2780
SinI GGWCC 3 cut(s) 301, 1789, 3245
SmiMI CAYNNNNRTG 4 cut(s) 1023, 2595, 2723, 3555
SmlI CTYRAG 2 cut(s) 877, 2255
SmoI CTYRAG 2 cut(s) 877, 2255
SphI GCATGC 1 cut(s) 2590
SsiI CCGC 7 cut(s) 623, 655, 733, 837, 1484, 1773, 2643
SspI AATATT 1 cut(s) 3529
SspMI CTAG 9 cut(s) 126, 470, 947, 1634, 2084, 2139, 2198, 2535, 3218
StyD4I CCNGG 5 cut(s) 303, 988, 1215, 1261, 1581
StyI CCWWGG 3 cut(s) 924, 1008, 2724
TaaI ACNGT 6 cut(s) 13, 716, 757, 2244, 2713, 3418
TaiI ACGT 3 cut(s) 446, 963, 2376
TaqI TCGA 5 cut(s) 639, 676, 1181, 2445, 2751
TatI WGTACW 3 cut(s) 166, 1777, 3497
TauI GCSGC 1 cut(s) 1775
Tru1I TTAA 8 cut(s) 1800, 2097, 2114, 2256, 2531, 3276, 3303, 3674
Tru9I TTAA 8 cut(s) 1800, 2097, 2114, 2256, 2531, 3276, 3303, 3674
TscAI CASTG 5 cut(s) 1346, 1984, 2350, 2420, 3615
TseFI GTSAC 4 cut(s) 1060, 1132, 2758, 3037
TseI GCWGC 8 cut(s) 205, 724, 727, 730, 1671, 2779, 3567, 3622
Tsp45I GTSAC 4 cut(s) 1060, 1132, 2758, 3037
TspGWI ACGGA 2 cut(s) 996, 1043
TspRI CASTG 5 cut(s) 1346, 1984, 2350, 2420, 3615
Van91I CCANNNNNTGG 2 cut(s) 1095, 3385
Vha464I CTTAAG 1 cut(s) 2255
VpaK11BI GGWCC 3 cut(s) 301, 1789, 3245
XapI RAATTY 8 cut(s) 517, 1154, 1257, 1353, 2030, 2276, 2938, 3279
XceI RCATGY 9 cut(s) 1022, 1759, 2413, 2590, 2594, 3156, 3260, 3560, 3601
XmiI GTMKAC 1 cut(s) 978
XmnI GAANNNNTTC 1 cut(s) 2564
XspI CTAG 9 cut(s) 126, 470, 947, 1634, 2084, 2139, 2198, 2535, 3218
ZrmI AGTACT 1 cut(s) 3499
Zsp2I ATGCAT 4 cut(s) 599, 1857, 2588, 2592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.