RchiOBHm_Chr5g0079591

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015588
Physical Location & Seq
Reverse (-)
85333592 .. 85344322
10731 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35398

Sequence Viewer

Length: 4146 bp
ATGGAGGGGAAACCAATTCTTGCGGTAATTGTTGCATATGTCAGTTACATGGCGATTAATAAGTGGGTATGGACTCCAATGAAGCGGGGCCCAAAAGCAATACGGAAGGAAGATGATGATTCACCAACTGTTCCTTCATCATTAGCTGAATCTTCGTCATTGTTATCACCTCCTTCTCCTGAGCGGAAGGATGATCCAGCATCTCTTCCTTCATCATCAGCTGAAGTATCAATATCGGAGGTTAATCAAAAATCGGTGGACCTCGATCCCTCTGTATCGCTTCTTTCTTCAGCTGAGTCACCATCAGCTCCCATGCTGAAAAACTCTGAGCCAATATCAGAGGTTAATCAAGAATCGGTGGACCTCGATCCCTCCACATCCCTTCTTTCATTAGCCGAGTCGTCATTATCAGCCTCCATGCGGGATAACCCTGAGCCAATATCGGATGTTAATCAAGAATTGGTTGACATCGATCTCTCTGCATCTCTTCTTTCTTCAGCTGAGTCATCATCAGCTCCCATGTTGGATAACCGTGAGCCAATATTAGAGGTAAATCAAGAATTGGAGGACCTCGATCCCTCTACATCTCTTCTTTCGTTAGCCGAGTCATCATCATCAGCTTCCATGCTGGAAATCCCTGAGCCAATATCGGAAGTTAATCAAGAATCAGTTGATGTCGCTCCCTTTGCATCTCTTCTTTCTTCAGCTGAGTCGTCATCATCAGCTTCCGTGCTGGAAAACCCTGAGCCAATATTGGAGGTTAATCAAGAATCGGTGGACCTCAGACCCTCTACATCTCTTTTTTCAGCTCCTTTGCAGAGGTATGATGTTTTTCTGAATTTCAGGGGTGAAGATACTCGTAAGAATTTTGTACCCCACTTATACAATGAATTGAAAGAGGTGAGAGGAATTGAAACGTTCAAGGATGACAGAGAGCTTGAAATAGGGGACCGTATTTTTGCAACTCTGCAAAAGGCAATTGAAGAATCAAAATGTGCAATTGTTGTTTTATCAAAAAGATATGCCGAATCTCGCTGGTGTTTGAAGGAACTTGTAAAGATTTGCCAATGCATGAAAGACAACAACAGGATTTTACCAATTTTTTATCACGTCGAACCTTGTGAGGTACGATTTCAGAAGGGTTGTTTTGCAGAGGCTTTCACTAAGCACCAAAACTCAGGGCGGCAAAGTTCAGAAGAGGTTGAGGAGTGGAGAAAAGCTTTAAGAACAGTTGCCGATTTCTCTGGGTGTAATACAAAGGATCATGGGGATGAAAGAGAAGTTATCGATCACATTGTGGAATCAGTGTGCAGTAAAATACAAACCATTGAAAGTGATCGTATATTTCCCTTGGGAAATTTTGTATCTTTTGAGGCAACAAGAGAAGCCATGAATAATGTAATGAAGGCGCTAGACGGTAATACTGCCGTTGGGATCTACGGCATGGGAGGCGTCGGAAAGACAACCATGGTGGAACATGTCGCTCGAGAAGTACTAAAGAATGGGCTTTTCCATCAGGTGACTATGGCCGTGATATCCCAAAAGCCCAACTATTGGAAGATTCAAGAAAGATTAGCTTCCATGTTGAAGGTGAAACTACAGAGTACGGACGAATTTGCAAGAGCTGCTACATTGCAGTCAAAGATTTGGGGCAGAGAAAAAATTCTTATAATCCTGGACGACATTTGGGCAGAAATACAGTTGAAACACATAGGAATTCCCAGCCTCGAAGAGCTTAAAAGACGCAATTCCACGGTCCTACTCACCACAAGGAATGAGGATGTTTGCTCTGATATGTGCTGCCAAGAAAAAATTCATCTGAATACCTTAAGTGAAAAAGATTCTTGGATGTTGTTTGAGAAGAATACAGGAATGTCTTTTCAAGAAAAAACCCAGTTCTGTTGCGTTGCAAAGGAGGTAGCAAGAGAATGTGCTGGTCTTCCGATTGCGTTGGAAGCAGTTGCAAAGGCACTTAAGAATAAAGATTTGAAGCAATGGAGGGCAGCTGTTGGCCGACTAAAGCTCTCCAGAACGCCCCATCCTGAATCCAACAGAGTTGTGTTCAACAGCATAAATTTAAGCTATGAGTACTTGAAATCTAATGACTCCAGATCATGCTTCTTGCTTTGCTGCCTATTCCCAGAGGATTATGATATCCAAATAGAAGACTTGCTGAAGTATGGGATTGGGAAAGGATTGTTTCAAGAAGAGTCCGACATGCAACAAGCCAGAGATGCAATATGCCTCGTGGTCCAGTCCCTTAAAGATTATAGCCTACTTTTGGATGGTAAGATGGGCGGGGGATGCGTAAGAATGCATGATGTCATCAGAGATGTTGGCTTATCAATTTCTTTATCTAAGGATGGATACAGAGATTTTTTTGTAAGAGCTGGTTGGAAATTAGAGGATTGGCCACTGAATGATGCGCCTGAATACTACACTGCAATCTCACTTATGAGGAACAAAATACCTGAGCTTCCTGAAGAGTTGGGATTTCCAGAACTCCAGATTCTCTTGTTACAAAGTAATTCTTTGAATGGGATCCCAGTTTCTTCTTTTCAACGTCTAAAGGTATTAAGGGTCTTAGATATTAGCCAGACTAACATTTCTTTACTACCCTCATCATCCAATCTCCTAACCAGCCTCCACACCTTGTACCTAGATGGTTGCTTGTCAATGGAAAGTAACATACCCCTACTTGGACAGTTGAAACAACTTAAGATTCTTAGCATGAGAGAATTTCCTCATCAGAAATTGCCGAAAGAAATAGGACAGTTGACCAAACTAAAGCTGCTGGACTTCACTAGTCAATCTGTTGAAATAGTTCCTCCTAAAGTGATAGGCAACCTCAGTAAGCTAGAAGAACTCTACATGAAGTGCTACTTTGCGGACTGGGGGAGTAGTGTTGAGGGGAGTAAAGTGAAGGGGAGTGAAGTCGAGGGAAATATAGATGAGGAGAACAAAGCTATGGGAGTAGGAGAGGAAACTAATGCTTTCTTTGATGAGTTAACTAACTTGTCAGGTCTACACATTTTGAATGTTGTCATTTCTGATGCAAATTGTATGCCTATAACTGTTAGGTGTAACCCGAAATGGATAAGCTTTGACATATGTATCAGCAGTGACCAGTCAGTTATAAAATGGCATTTGGATTCAAGTTCTTCACCTGACTATCCAAAAGCCTTGACCCTTGGCATAACCATGGATACCTTACGGAAGTGGGAGTGGTTTATCGATGTTGTGACCAAGAAAACAGAGAAGCTACAGTATAAAAATTGCCGAGATTTAGGTAACATCCTTATGGAATATGAGTGGGGGAGATTACATGAACTGAAGTATTTATCTGTTATTGGGCCCAATGAGAAATTGAAAGAGTTGATCAACACAAAAACATGGGTTCAAAATGAACCAGTGTTTCAGAACTTAGAGGAGTTGCATCTCATTCAAGTGGATAGCCTGAAGGAGTTATGCGTTGGTGAATTACCACTTGGGTCTCTATGCAATCTTAGGTTATTGAAGGTGAAACATTGTTATGAATTGGTGGATGCACTTCTATCTTCACAATTGCTGCAGAGATTACAAAATCTGGAAAAACTGATTTGTGAAGAGATGGATGAATTGGAATATGTTTTTGGATGTGAAGGGCTTAAGCCAGAACATATTATTATGACAGAATTGAAAGAGATGAGATTGGAAAATCTAGGGAAACTAATAAAGATATGGAATGGTCCTGCCCCTTGTGCCATTTTCCAAAACCTTCAGAGTTTGGTAGTTTCAGGATGCTTTGATTTGGAAAATCTCTTCACAGCTGATGTTGCTCAGTGTCTTTTGCAATTGCAAGACCTTTCAGTAGAGCTTTGCCCATTATTGGACAGAGTTATTGAGGAAAGCAAGGAAACAATAGACAGCAAGATTGTTTTTCCAAAAATGAAGAGCTTAGCTTTGATACAGCTACCACAGCTCACAAGGTTGTATGGTAATACTGGAAGTGCTATTGAGTGCCCTTTATTAGAATACTTGTACGTGGAGAGGTGCCCCCAGTTCTCAATACCTGACTTCCACAGCAGGAACAGAGTACAATTCAATCGTCAACAACATTTTGACATTATAGAAAGAAGATTGCTGAGAAACAAAAGAAGGCCCTGA

Protein Analysis

1381

Amino Acids

155.79

Weight (kDa)

5.08

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 275 - 441 1e-48 TIR domain
TIR_2 PF13676 277 - 373 2.1e-15 TIR domain
NB-ARC PF00931 464 - 624 5.3e-29 NB-ARC domain
LRR_8 PF13855 833 - 890 3.8e-08 Leucine rich repeat
LRR_14 PF23598 882 - 960 3.5e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 1142 - 1287 3.1e-28 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1670, 3137
AccB1I GGYRCC 1 cut(s) 4032
AccB7I CCANNNNNTGG 1 cut(s) 1554
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 3025
AciI CCGC 7 cut(s) 23, 85, 184, 421, 1183, 2298, 2888
AclI AACGTT 1 cut(s) 917
AclWI GGATC 8 cut(s) 188, 260, 362, 569, 1269, 1442, 2536, 2549
AcoI YGGCCR 3 cut(s) 1527, 2011, 2411
AcsI RAATTY 9 cut(s) 838, 865, 1357, 1613, 1662, 1716, 1812, 2074, 2738
AcuI CTGAAG 9 cut(s) 243, 273, 480, 687, 2195, 2502, 3353, 3479, 3743
AcyI GRCGYC 1 cut(s) 1452
AdeI CACNNNGTG 1 cut(s) 1297
AfaI GTAC 8 cut(s) 873, 1128, 1494, 1606, 2090, 2657, 4022, 4077
AfiI CCNNNNNNNGG 5 cut(s) 420, 1554, 2281, 2699, 3868
AflII CTTAAG 4 cut(s) 1828, 1973, 2717, 3647
AflIII ACRYGT 1 cut(s) 1477
AhlI ACTAGT 1 cut(s) 2804
AjiI CACGTC 1 cut(s) 1111
AjnI CCWGG 1 cut(s) 1674
AjuI GAANNNNNNNTTGG 8 cut(s) 118, 150, 1797, 1829, 3471, 3503, 3615, 3647
AloI GAACNNNNNNTCC 2 cut(s) 2023, 2055
Alw26I GTCTC 1 cut(s) 3498
AlwI GGATC 8 cut(s) 188, 260, 362, 569, 1269, 1442, 2536, 2549
Ama87I CYCGRG 1 cut(s) 1485
AoxI GGCC 6 cut(s) 88, 1527, 2011, 2411, 3353, 4139
ApaI GGGCCC 2 cut(s) 92, 3357
ApeKI GCWGC 6 cut(s) 1625, 1800, 2003, 2130, 2791, 3568
ApoI RAATTY 9 cut(s) 838, 865, 1357, 1613, 1662, 1716, 1812, 2074, 2738
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AseI ATTAAT 1 cut(s) 57
Asp700I GAANNNNTTC 3 cut(s) 1662, 1812, 2823
AspLEI GCGC 2 cut(s) 1411, 2428
AvaI CYCGRG 1 cut(s) 1485
AvaII GGWCC 8 cut(s) 259, 361, 568, 778, 949, 1756, 2251, 3728
BaeGI GKGCMC 4 cut(s) 92, 3357, 4004, 4037
BaeI ACNNNNGTAYC 2 cut(s) 3097, 3130
BalI TGGCCA 1 cut(s) 2413
BamHI GGATCC 1 cut(s) 2541
BanI GGYRCC 1 cut(s) 4032
BanII GRGCYC 2 cut(s) 92, 3357
BauI CACGAG 1 cut(s) 2246
BbsI GAAGAC 2 cut(s) 1931, 2172
BbvI GCAGC 6 cut(s) 1612, 1787, 2015, 2117, 2778, 3555
BccI CCATC 8 cut(s) 310, 1521, 2046, 2279, 2287, 2357, 2657, 3604
BceAI ACGGC 3 cut(s) 1412, 1456, 1514
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 1 cut(s) 1676
BciVI GTATCC 2 cut(s) 2360, 3199
BclI TGATCA 1 cut(s) 3378
BcoDI GTCTC 1 cut(s) 3498
BcuI ACTAGT 1 cut(s) 2804
BfaI CTAG 5 cut(s) 1412, 2660, 2805, 2858, 3701
BfmI CTRYAG 3 cut(s) 1598, 3263, 3569
BfoI RGCGCY 1 cut(s) 1412
BfrI CTTAAG 4 cut(s) 1828, 1973, 2717, 3647
BfuI GTATCC 2 cut(s) 2360, 3199
BisI GCNGC 7 cut(s) 1184, 1626, 1801, 2004, 2131, 2792, 3569
BlpI GCTNAGC 1 cut(s) 3937
BlsI GCNGC 7 cut(s) 1185, 1627, 1802, 2005, 2132, 2793, 3570
BmcAI AGTACT 2 cut(s) 1494, 2090
Bme1390I CCNGG 1 cut(s) 1676
Bme18I GGWCC 8 cut(s) 259, 361, 568, 778, 949, 1756, 2251, 3728
BmeT110I CYCGRG 1 cut(s) 1485
BmgBI CACGTC 1 cut(s) 1111
BmiI GGNNCC 6 cut(s) 89, 90, 950, 2543, 3355, 4034
BmrFI CCNGG 1 cut(s) 1676
BmrI ACTGGG 4 cut(s) 1888, 2540, 2902, 4033
BmuI ACTGGG 4 cut(s) 1888, 2540, 2902, 4033
BpiI GAAGAC 2 cut(s) 1931, 2172
BpmI CTGGAG 3 cut(s) 2011, 2092, 2489
Bpu10I CCTNAGC 5 cut(s) 180, 432, 639, 744, 2472
Bpu1102I GCTNAGC 1 cut(s) 3937
Bsa29I ATCGAT 3 cut(s) 471, 1287, 3234
BsaAI YACGTR 1 cut(s) 4024
BsaBI GATNNNNATC 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 1452
BsaI GGTCTC 1 cut(s) 3498
BsaJI CCNNGG 5 cut(s) 1350, 1467, 1752, 3190, 3201
Bsc4I CCNNNNNNNGG 5 cut(s) 420, 1554, 2281, 2699, 3868
Bse1I ACTGG 8 cut(s) 1894, 2254, 2546, 2897, 3127, 3410, 3988, 4039
Bse3DI GCAATG 2 cut(s) 1631, 2000
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 1 cut(s) 1676
BseCI ATCGAT 3 cut(s) 471, 1287, 3234
BseDI CCNNGG 5 cut(s) 1350, 1467, 1752, 3190, 3201
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 5 cut(s) 420, 1554, 2281, 2699, 3868
BseMI GCAATG 2 cut(s) 1631, 2000
BseNI ACTGG 8 cut(s) 1894, 2254, 2546, 2897, 3127, 3410, 3988, 4039
BseRI GAGGAG 3 cut(s) 1220, 2969, 3443
BseSI GKGCMC 4 cut(s) 92, 3357, 4004, 4037
BseXI GCAGC 6 cut(s) 1612, 1787, 2015, 2117, 2778, 3555
BseYI CCCAGC 1 cut(s) 1721
BsgI GTGCAG 1 cut(s) 1330
BshFI GGCC 6 cut(s) 90, 1529, 2013, 2413, 3355, 4141
BshNI GGYRCC 1 cut(s) 4032
BshVI ATCGAT 3 cut(s) 471, 1287, 3234
BsiHKCI CYCGRG 1 cut(s) 1485
BslFI GGGAC 2 cut(s) 962, 2242
BslI CCNNNNNNNGG 5 cut(s) 420, 1554, 2281, 2699, 3868
BsmAI GTCTC 1 cut(s) 3498
BsmFI GGGAC 2 cut(s) 962, 2242
BsmI GAATGC 1 cut(s) 2319
BsnI GGCC 6 cut(s) 90, 1529, 2013, 2413, 3355, 4141
Bso31I GGTCTC 1 cut(s) 3498
BsoBI CYCGRG 1 cut(s) 1485
Bsp120I GGGCCC 2 cut(s) 88, 3353
Bsp1286I GDGCHC 4 cut(s) 92, 3357, 4004, 4037
Bsp1720I GCTNAGC 1 cut(s) 3937
Bsp19I CCATGG 2 cut(s) 1467, 3201
BspACI CCGC 7 cut(s) 23, 85, 184, 421, 1183, 2298, 2888
BspANI GGCC 6 cut(s) 90, 1529, 2013, 2413, 3355, 4141
BspDI ATCGAT 3 cut(s) 471, 1287, 3234
BspLI GGNNCC 6 cut(s) 89, 90, 950, 2543, 3355, 4034
BspMAI CTGCAG 1 cut(s) 3573
BspPI GGATC 8 cut(s) 188, 260, 362, 569, 1269, 1442, 2536, 2549
BspQI GCTCTTC 2 cut(s) 1725, 3926
BspT107I GGYRCC 1 cut(s) 4032
BspTI CTTAAG 4 cut(s) 1828, 1973, 2717, 3647
BspTNI GGTCTC 1 cut(s) 3498
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 1631, 2000
BsrI ACTGG 8 cut(s) 1894, 2254, 2546, 2897, 3127, 3410, 3988, 4039
BssECI CCNNGG 5 cut(s) 1350, 1467, 1752, 3190, 3201
BssNI GRCGYC 1 cut(s) 1452
BssSI CACGAG 1 cut(s) 2246
BssT1I CCWWGG 4 cut(s) 1350, 1467, 3190, 3201
Bst2BI CACGAG 1 cut(s) 2246
Bst2UI CCWGG 1 cut(s) 1676
BstACI GRCGYC 1 cut(s) 1452
BstAFI CTTAAG 4 cut(s) 1828, 1973, 2717, 3647
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstBAI YACGTR 1 cut(s) 4024
BstDSI CCRYGG 3 cut(s) 1467, 1752, 3201
BstH2I RGCGCY 1 cut(s) 1412
BstHHI GCGC 2 cut(s) 1411, 2428
BstMAI GTCTC 1 cut(s) 3498
BstMWI GCNNNNNNNGC 9 cut(s) 686, 1449, 1625, 1955, 2234, 2304, 3740, 3815, 3958
BstNI CCWGG 1 cut(s) 1676
BstNSI RCATGY 2 cut(s) 1481, 2221
BstSCI CCNGG 1 cut(s) 1674
BstSFI CTRYAG 3 cut(s) 1598, 3263, 3569
BstSLI GKGCMC 4 cut(s) 92, 3357, 4004, 4037
BstV1I GCAGC 6 cut(s) 1612, 1787, 2015, 2117, 2778, 3555
BstV2I GAAGAC 2 cut(s) 1931, 2172
BstX2I RGATCY 2 cut(s) 1434, 2541
BstYI RGATCY 2 cut(s) 1434, 2541
Bsu15I ATCGAT 3 cut(s) 471, 1287, 3234
BsuI GTATCC 2 cut(s) 2360, 3199
BsuRI GGCC 6 cut(s) 90, 1529, 2013, 2413, 3355, 4141
BsuTUI ATCGAT 3 cut(s) 471, 1287, 3234
BtgI CCRYGG 3 cut(s) 1467, 1752, 3201
BtrI CACGTC 1 cut(s) 1111
BtsI GCAGTG 2 cut(s) 2439, 3127
BtsIMutI CAGTG 6 cut(s) 1311, 2414, 2439, 3127, 3417, 3827
CfoI GCGC 2 cut(s) 1411, 2428
ClaI ATCGAT 3 cut(s) 471, 1287, 3234
CseI GACGC 2 cut(s) 1441, 1752
Csp6I GTAC 8 cut(s) 872, 1127, 1493, 1605, 2089, 2656, 4021, 4076
CspCI CAANNNNNGTGG 2 cut(s) 1452, 1487
CviQI GTAC 8 cut(s) 872, 1127, 1493, 1605, 2089, 2656, 4021, 4076
DraIII CACNNNGTG 1 cut(s) 1297
EaeI YGGCCR 3 cut(s) 1527, 2011, 2411
Eco130I CCWWGG 4 cut(s) 1350, 1467, 3190, 3201
Eco24I GRGCYC 2 cut(s) 92, 3357
Eco31I GGTCTC 1 cut(s) 3498
Eco32I GATATC 2 cut(s) 1536, 2155
Eco47I GGWCC 8 cut(s) 259, 361, 568, 778, 949, 1756, 2251, 3728
Eco57I CTGAAG 9 cut(s) 243, 273, 480, 687, 2195, 2502, 3353, 3479, 3743
Eco88I CYCGRG 1 cut(s) 1485
EcoO109I RGGNCCY 3 cut(s) 88, 568, 4140
EcoRI GAATTC 1 cut(s) 1716
EcoRII CCWGG 1 cut(s) 1674
EcoRV GATATC 2 cut(s) 1536, 2155
EcoT14I CCWWGG 4 cut(s) 1350, 1467, 3190, 3201
EcoT22I ATGCAT 2 cut(s) 1073, 2319
EcoT38I GRGCYC 2 cut(s) 92, 3357
ErhI CCWWGG 4 cut(s) 1350, 1467, 3190, 3201
FalI AAGNNNNNCTT 6 cut(s) 1561, 1593, 2515, 2547, 2867, 2899
FaqI GGGAC 2 cut(s) 962, 2242
FauI CCCGC 3 cut(s) 78, 414, 2291
FauNDI CATATG 2 cut(s) 37, 3110
FbaI TGATCA 1 cut(s) 3378
FblI GTMKAC 1 cut(s) 3025
Fnu4HI GCNGC 7 cut(s) 1184, 1626, 1801, 2004, 2131, 2792, 3569
FriOI GRGCYC 2 cut(s) 92, 3357
Fsp4HI GCNGC 7 cut(s) 1184, 1626, 1801, 2004, 2131, 2792, 3569
FspBI CTAG 5 cut(s) 1412, 2660, 2805, 2858, 3701
GlaI GCGC 2 cut(s) 1410, 2427
GluI GCNGC 7 cut(s) 1184, 1626, 1801, 2004, 2131, 2792, 3569
GsaI CCCAGC 1 cut(s) 1725
GsuI CTGGAG 3 cut(s) 2011, 2092, 2489
HaeII RGCGCY 1 cut(s) 1412
HaeIII GGCC 6 cut(s) 90, 1529, 2013, 2413, 3355, 4141
HgaI GACGC 2 cut(s) 1441, 1752
HhaI GCGC 2 cut(s) 1411, 2428
Hin1I GRCGYC 1 cut(s) 1452
Hin6I GCGC 2 cut(s) 1409, 2426
HinP1I GCGC 2 cut(s) 1409, 2426
HincII GTYRAC 4 cut(s) 466, 2778, 3009, 4091
HindII GTYRAC 4 cut(s) 466, 2778, 3009, 4091
HindIII AAGCTT 2 cut(s) 1218, 3100
HpaI GTTAAC 1 cut(s) 3009
Hpy166II GTNNAC 8 cut(s) 259, 361, 466, 778, 2778, 3009, 3026, 4091
Hpy8I GTNNAC 8 cut(s) 259, 361, 466, 778, 2778, 3009, 3026, 4091
Hpy99I CGWCG 2 cut(s) 1115, 1457
HpyCH4IV ACGT 4 cut(s) 917, 1110, 2563, 4023
HpyF10VI GCNNNNNNNGC 9 cut(s) 686, 1449, 1625, 1955, 2234, 2304, 3740, 3815, 3958
HpySE526I ACGT 4 cut(s) 917, 1110, 2563, 4023
Hsp92I GRCGYC 1 cut(s) 1452
HspAI GCGC 2 cut(s) 1409, 2426
Ksp22I TGATCA 1 cut(s) 3378
KspAI GTTAAC 1 cut(s) 3009
LguI GCTCTTC 2 cut(s) 1725, 3926
LmnI GCTCC 4 cut(s) 313, 520, 685, 814
Lsp1109I GCAGC 6 cut(s) 1612, 1787, 2015, 2117, 2778, 3555
MaeI CTAG 5 cut(s) 1412, 2660, 2805, 2858, 3701
MaeII ACGT 4 cut(s) 917, 1110, 2563, 4023
MaeIII GTNAC 9 cut(s) 44, 297, 1519, 2517, 2684, 3083, 3122, 3241, 3290
MbiI CCGCTC 1 cut(s) 184
MfeI CAATTG 4 cut(s) 978, 999, 3563, 3833
MflI RGATCY 2 cut(s) 1434, 2541
MhlI GDGCHC 4 cut(s) 92, 3357, 4004, 4037
MlsI TGGCCA 1 cut(s) 2413
MluNI TGGCCA 1 cut(s) 2413
MlyI GAGTC 8 cut(s) 67, 305, 407, 512, 614, 719, 2099, 2219
MmeI TCCRAC 6 cut(s) 504, 1435, 1932, 2073, 2238, 2375
Mox20I TGGCCA 1 cut(s) 2413
Mph1103I ATGCAT 2 cut(s) 1073, 2319
MroXI GAANNNNTTC 3 cut(s) 1662, 1812, 2823
MscI TGGCCA 1 cut(s) 2413
MslI CAYNNNNRTG 5 cut(s) 1269, 3200, 3299, 3446, 3531
Msp20I TGGCCA 1 cut(s) 2413
MspA1I CMGCKG 6 cut(s) 221, 293, 500, 707, 2006, 3809
MspCI CTTAAG 4 cut(s) 1828, 1973, 2717, 3647
MspR9I CCNGG 1 cut(s) 1676
MunI CAATTG 4 cut(s) 978, 999, 3563, 3833
Mva1269I GAATGC 1 cut(s) 2319
MvaI CCWGG 1 cut(s) 1676
MwoI GCNNNNNNNGC 9 cut(s) 686, 1449, 1625, 1955, 2234, 2304, 3740, 3815, 3958
NcoI CCATGG 2 cut(s) 1467, 3201
NdeI CATATG 2 cut(s) 37, 3110
NlaIV GGNNCC 6 cut(s) 89, 90, 950, 2543, 3355, 4034
NmeAIII GCCGAG 3 cut(s) 421, 628, 3305
NmuCI GTSAC 4 cut(s) 297, 1519, 3122, 3241
NsiI ATGCAT 2 cut(s) 1073, 2319
NspI RCATGY 2 cut(s) 1481, 2221
PaeR7I CTCGAG 1 cut(s) 1485
PciI ACATGT 1 cut(s) 1477
PciSI GCTCTTC 2 cut(s) 1725, 3926
PctI GAATGC 1 cut(s) 2319
PdmI GAANNNNTTC 3 cut(s) 1662, 1812, 2823
PflMI CCANNNNNTGG 1 cut(s) 1554
PfoI TCCNGGA 1 cut(s) 1674
PkrI GCNGC 7 cut(s) 1185, 1627, 1802, 2005, 2132, 2793, 3570
PleI GAGTC 8 cut(s) 67, 304, 406, 511, 613, 718, 2099, 2218
PpsI GAGTC 8 cut(s) 67, 304, 406, 511, 613, 718, 2099, 2218
Ppu21I YACGTR 1 cut(s) 4024
PpuMI RGGWCCY 1 cut(s) 568
PscI ACATGT 1 cut(s) 1477
PshBI ATTAAT 1 cut(s) 57
PsiI TTATAA 2 cut(s) 1670, 3137
Psp1406I AACGTT 1 cut(s) 917
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 1674
PspFI CCCAGC 1 cut(s) 1721
PspGI CCWGG 1 cut(s) 1674
PspN4I GGNNCC 6 cut(s) 89, 90, 950, 2543, 3355, 4034
PspOMI GGGCCC 2 cut(s) 88, 3353
PspPPI RGGWCCY 1 cut(s) 568
PstI CTGCAG 1 cut(s) 3573
PsuI RGATCY 2 cut(s) 1434, 2541
PvuII CAGCTG 6 cut(s) 221, 293, 500, 707, 2006, 3809
RsaI GTAC 8 cut(s) 873, 1128, 1494, 1606, 2090, 2657, 4022, 4077
RsaNI GTAC 8 cut(s) 872, 1127, 1493, 1605, 2089, 2656, 4021, 4076
RseI CAYNNNNRTG 5 cut(s) 1269, 3200, 3299, 3446, 3531
SapI GCTCTTC 2 cut(s) 1725, 3926
SatI GCNGC 7 cut(s) 1184, 1626, 1801, 2004, 2131, 2792, 3569
ScaI AGTACT 2 cut(s) 1494, 2090
SchI GAGTC 8 cut(s) 67, 305, 407, 512, 614, 719, 2099, 2219
ScrFI CCNGG 1 cut(s) 1676
SduI GDGCHC 4 cut(s) 92, 3357, 4004, 4037
SfcI CTRYAG 3 cut(s) 1598, 3263, 3569
Sfr274I CTCGAG 1 cut(s) 1485
SinI GGWCC 8 cut(s) 259, 361, 568, 778, 949, 1756, 2251, 3728
SlaI CTCGAG 1 cut(s) 1485
SmiMI CAYNNNNRTG 5 cut(s) 1269, 3200, 3299, 3446, 3531
SmlI CTYRAG 5 cut(s) 1485, 1828, 1973, 2717, 3647
SmoI CTYRAG 5 cut(s) 1485, 1828, 1973, 2717, 3647
SpeI ACTAGT 1 cut(s) 2804
SsiI CCGC 7 cut(s) 23, 85, 184, 421, 1183, 2298, 2888
SspI AATATT 2 cut(s) 543, 753
SspMI CTAG 5 cut(s) 1412, 2660, 2805, 2858, 3701
StyD4I CCNGG 1 cut(s) 1674
StyI CCWWGG 4 cut(s) 1350, 1467, 3190, 3201
TaiI ACGT 4 cut(s) 920, 1113, 2566, 4026
TatI WGTACW 3 cut(s) 1492, 2088, 4075
TauI GCSGC 1 cut(s) 1186
TscAI CASTG 6 cut(s) 1311, 2421, 2446, 3127, 3417, 3827
TseFI GTSAC 4 cut(s) 297, 1519, 3122, 3241
TseI GCWGC 6 cut(s) 1625, 1800, 2003, 2130, 2791, 3568
Tsp45I GTSAC 4 cut(s) 297, 1519, 3122, 3241
TspGWI ACGGA 4 cut(s) 118, 718, 1622, 3229
TspRI CASTG 6 cut(s) 1311, 2421, 2446, 3127, 3417, 3827
Van91I CCANNNNNTGG 1 cut(s) 1554
Vha464I CTTAAG 4 cut(s) 1828, 1973, 2717, 3647
VpaK11BI GGWCC 8 cut(s) 259, 361, 568, 778, 949, 1756, 2251, 3728
VspI ATTAAT 1 cut(s) 57
XapI RAATTY 9 cut(s) 838, 865, 1357, 1613, 1662, 1716, 1812, 2074, 2738
XceI RCATGY 2 cut(s) 1481, 2221
XhoI CTCGAG 1 cut(s) 1485
XmiI GTMKAC 1 cut(s) 3025
XmnI GAANNNNTTC 3 cut(s) 1662, 1812, 2823
XspI CTAG 5 cut(s) 1412, 2660, 2805, 2858, 3701
ZrmI AGTACT 2 cut(s) 1494, 2090
Zsp2I ATGCAT 2 cut(s) 1073, 2319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.