Rw5G048480

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015588
Physical Location & Seq
Reverse (-)
84296371 .. 84300519
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048480.1

Sequence Viewer

Length: 3630 bp
ATGGAGCTCTATTCCGTCGAGATGGCGTTGGCGATAGCTGTGGGATTTGCGACAATGACACATTGGTTGGCTTCCATGGTTGAAAACCGTGAGCCAGAGTCTAATGATAATCGAGAACTGGCCGACGTGGACCCCCCTGCATCTCTTCTTTCATCAGCTGAATCATCGTCAGCTTCCATGGTTGAAAACCCAGAGCCAATACCGGAGGAAAATCAAGAATTGGTCCCCTCTACATCTCTTGTATCATCAGCAGCTCCTTGGCAGAAGTATGATGTTTTTCTGAGTTTCAGGGGTGCAGACACTCGTACGGGTTTTGTATCCCACTTATACCATGAATTGCAGACTGTGAGAGGGATTCAAACGTTCATGGATGACAAAAAGCTTGAACTAGGGGCTCCTATTTCTCGAAGTCTGCTAGAGGCAATTGAAGAATCAAATTTTGCAATTGTTGTTTTGTCGAAAGACTATGCTCAATCTGCATGGTGTTTGGAGGAACTTACAAAGATTTGTCAATGCATGAAAACCAATAACAGAATCCTACCCCTGTTTTATCATGTCGAACCTATGGATGTGCGGCATCAAAAGGGGACTTTTGGAGATGCTTTCACAAATCATGTGAAAAATGGGTGGCACAGTTCAGAGAAGATGCAGCAGTGGAGAAAAGCTTTAAAAACAGTAGCCAGTTTCTCTGGGTGTAATGCAAAGGATCATCAGAATGAAAGAGATCTTGTCGAAGCCATTGTGGAATCTGTGTGCAGTAAAGTACGACCTATTGAAATTGAGTTTATTATTTCCTTCAGAGATTTTGTACCATTTAAAGCAACCAGAGAAGCCATGGACAATGTACTGAAGGCGCTAAAAGATGAAAAGGTCACTGCCGTTGGGGTGTACGGCATGGGAGGCATTGGAAAAACAACCATGGTGGAACATGTTGGTGTAGAAGCCCAAAAAAAGGGGATTTTCCGTCAGGTGATTATGGCTGGCATATCCCAAAAGCCTAACTTGTGGAATATTCAAGACACATTAGCAGACATGTTGGGTGTGAAGCTGGTGGATGCGACCATAACTGGAAGAGCCAAAACACTGAGGAAGAAGATAAGGAGTGGAAATAGGATCCTTATAATTTTGGACAACATTTGGAATGAAATACAGTTGACAGACATAGGAATTCCCAGATCCAACGAGCTTAAAAGATGCAATTCCAAGGTCCTACTCACAACAAGGAAAAGGGATGTTTGCTATCGTATGCAATGCCAAGCAAGCATTCGTCTCAATACCTTAACTGAACAAGATTCTTGGAAGTTGTTTGAGAAGGAAGCAAGAAATTCTTTTGAAACGTCGACTTTCCATAACAAAGCAAGGCAGGTAGCAGGAGAATGTGGTGGTCTTCCAGTTGCATTGAAAGCAGTTGCAAAGGCACTTGGGGATAAAAAGTTGGACGAATGGAACAAAGCTGCTGAGCGACTAAAGGTGTCTCAACTTCCCCACCCTGAAGACGAGAAAGTTGTGTTCGAATGCATTAAGTTAAGCTATGATTACTTGAAATCTGATGTTTCCAAATCATGCTTTTTGCTTTGCTGCCTATTCCCGGAGGATGATGATATCAAAATTGAAGACTTGCTGAAGTGTGGGATTGGGAAAGGATTGTTTGAAGAGTCCAACATGCAAAATGCCAGAGACACAATACAGACCGTTGTCGAGTCTCTTAAAGATTCGGGCTTGCTTTTGGATGCTAAAAAAGAAGGGGGATATGTAAGGATGCATGATGTCGTCAGAGATGTTGCCATACAAATTTCCTTACAGTTTTTTGTAAGAGCTGGTTGTGAATTGCAGTATTGGCCAAGGATTGATGCAGGTAAAGGCTACACTGCAATCTCACTAATGAAGAACAAAATATGCATGCTTCCTGCTGAACACTTGGTATTTCCAGAACTTCGTATTCTCTTGTTACGAAGTAATATTATGAATAACATCCCAGTTTCTTCATTTGAACGTCTAGAGGCATTAAGGGTCTTAGATCTTAGCTACACTGGCATTTCTTTACTACCCACATCATTCAATCTCCTAACCAGCCTCCACACTTTGTATCTAGATTGTTGCTGCTTATCAAGGATAAGTAACATATCCCTACTTGGACAGTTGAAAAAGCTTGAGATTCTTAGTATGAGAGAACTTCCTCTTTTGAAATTGCCAAAAGAAATAGGACAGTTGACCAGACTAAGGATGCTAGACTTCACTAGTGTCTGTGTTGATATAGTTCCTTCTAAAGTGATAAGCAACCTCAATAAATTAGAGGAACTGTACATACAGTGTTATTTTGCGGACTGGGGGAGTAAAGTCAAGGGGAGTAAAGCAGAGGGGAATGAATTCAAGGGAAATACAGTTGAGGAGAGCGAAGCTGAGGGAGTAAGAGAGGAAACTAATGCTTCCTTTGATGAGTTAACTAGCTTGTTACATCTACACATTTTGAAGGTTTTCATATCTGATCCAAAATGCTTACCTACAGATGTTGAGGTCAAGTCTAATTGGAAAAGTTTTGACATATGTATCTGTAGAGACCGGTCAATTAGAGAATTGCTTTTGGATCCAAGATCTCCACTTAGAGAATTGCTTTTGAATTCAAGTTCTCCACTTGATTCAAAAGCCTTGACTCTTGACACAACCATGGATACCTTACCGCAATGGTTTACTGATGTGGTGAACAAGAAAACGGAGAAGCTGCAGTACATAAACTGTCAGGGTTTAGTTAATGTTCTTAATGAATATTACCAAGGGACATTACATGCACTTAAGTATTTACATGTTATTGGTCCCAGTGATAAATTGGAACAGTTGATGACCCCAATAACATCGGTTCCAAATGAACCTGTGTTTGAGAATTTGGAAGAGTTGCATCTCGTTGAAGTGATTTGTCTGAAGGAGTTATGTGTCGGTGAATTACCAAATGAGTCTCTATGCAAGCTTAGGTTACTGAAGGTGAGAGGTTGTCATGAATTGATGAATGCACTTTTACAATCTAATTTGCTGAAGAGACTAAAAAATTTGGAAAAACTAAATTGTGAAGAGATGGATAAATTGGAGTATGTATTTTCATCTGAAGGGTCTGAGGTAGAACAAAATACTCTGACAGAGTTGATAGAGATGAGATTGGAGAATCTAGGGAGCCTAATAAAGATATGGAATGGTCCTGCTCCTAGTGAAATCTTTTGTCATGTTAAAACTTTGGTAGTTTTCGAGTGCAATAAGCTGAAAAATCTTTTCACATCCGACATAGCTCACCATCTTTATGACTTGGAAGATCTTTCAGTAGAGGCTTGCCTACGGTTGGAGAGAGTTATTGAAGCAAGTGAGGAAACAGTGAACGACAAGATAGTTTTACCAAAATTGAAGAAGTTAGCTATGAAAAAGCTTCTTAAGCTTACAAGGTTCTATGGTAGTACTGGAAGTGCTACTAGTGATGAAGAATATGTTGAGTTTCCTTTATTGGAACACTCGCATGTGGAACGCTGCCATATTTTCAATTCTCCAGCTAAGGACATGTTGGTTTCGATGATGAATAAGCAGCCTGTTTTGATTCCTATGCAAGAGACAGGTCCGAGAGACACACCAACTTTAAGCAGAAGAAGATGA

Protein Analysis

1209

Amino Acids

136.9

Weight (kDa)

5.88

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 89 - 256 3.3e-49 TIR domain
TIR_2 PF13676 92 - 188 1.1e-15 TIR domain
NB-ARC PF00931 277 - 440 9e-29 NB-ARC domain
LRR_14 PF23598 627 - 776 5.3e-11 Leucine-rich repeat region
LRR_8 PF13855 643 - 698 2e-07 Leucine rich repeat
LRR_14 PF23598 701 - 965 1.6e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 961 - 1106 1e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1045 - 1173 1e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1121
Acc36I ACCTGC 2 cut(s) 1354, 1844
AccI GTMKAC 1 cut(s) 1340
AciI CCGC 3 cut(s) 574, 2321, 2678
AclI AACGTT 1 cut(s) 362
AclWI GGATC 7 cut(s) 714, 1108, 1121, 1170, 2480, 2579, 2592
AcoI YGGCCR 2 cut(s) 120, 1838
AcsI RAATTY 8 cut(s) 436, 1167, 1324, 1791, 2366, 2617, 2878, 3040
AcuI CTGAAG 8 cut(s) 781, 869, 1512, 1643, 2936, 2993, 3047, 3117
AfaI GTAC 8 cut(s) 307, 765, 810, 846, 890, 2303, 2726, 3439
AfiI CCNNNNNNNGG 4 cut(s) 952, 1588, 2220, 3325
AflII CTTAAG 2 cut(s) 2789, 3413
AflIII ACRYGT 4 cut(s) 928, 1032, 2800, 3537
AgeI ACCGGT 1 cut(s) 2559
AhlI ACTAGT 2 cut(s) 2237, 3452
AjiI CACGTC 1 cut(s) 127
AjuI GAANNNNNNNTTGG 2 cut(s) 1248, 1280
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 9 cut(s) 1274, 1479, 1671, 1707, 2550, 2955, 3025, 3581, 3594
AlwI GGATC 7 cut(s) 714, 1108, 1121, 1170, 2480, 2579, 2592
AoxI GGCC 2 cut(s) 120, 1838
ApeKI GCWGC 8 cut(s) 251, 649, 1454, 1578, 2100, 2719, 3507, 3562
ApoI RAATTY 8 cut(s) 436, 1167, 1324, 1791, 2366, 2617, 2878, 3040
AsiGI ACCGGT 1 cut(s) 2559
Asp700I GAANNNNTTC 2 cut(s) 2366, 2474
AspLEI GCGC 1 cut(s) 856
AspS9I GGNCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
AsuC2I CCSGG 1 cut(s) 1589
AsuHPI GGTGA 5 cut(s) 982, 2710, 2945, 2989, 3269
AsuII TTCGAA 1 cut(s) 1512
AvaII GGWCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
BaeI ACNNNNGTAYC 2 cut(s) 2530, 2563
BalI TGGCCA 1 cut(s) 1840
BamHI GGATCC 2 cut(s) 1113, 2584
BanII GRGCYC 2 cut(s) 9, 397
BarI GAAGNNNNNNTAC 2 cut(s) 2708, 2740
BbsI GAAGAC 3 cut(s) 1379, 1500, 1620
Bbv12I GWGCWC 1 cut(s) 9
BbvCI CCTCAGC 1 cut(s) 2400
BbvI GCAGC 8 cut(s) 263, 661, 1441, 1565, 2087, 2706, 3494, 3574
BccI CCATC 3 cut(s) 16, 3061, 3288
BceAI ACGGC 2 cut(s) 863, 907
BciVI GTATCC 2 cut(s) 328, 2662
BcnI CCSGG 1 cut(s) 1589
BcoDI GTCTC 9 cut(s) 1274, 1479, 1671, 1707, 2550, 2955, 3025, 3581, 3594
BcuI ACTAGT 2 cut(s) 2237, 3452
BfmI CTRYAG 3 cut(s) 2502, 2551, 2720
BfoI RGCGCY 1 cut(s) 857
BfrI CTTAAG 2 cut(s) 2789, 3413
BfuAI ACCTGC 2 cut(s) 1354, 1844
BfuI GTATCC 2 cut(s) 328, 2662
BglII AGATCT 4 cut(s) 724, 2017, 2591, 3298
BisI GCNGC 9 cut(s) 252, 575, 650, 1455, 1579, 2101, 2720, 3508, 3563
BlpI GCTNAGC 1 cut(s) 1458
BlsI GCNGC 9 cut(s) 253, 576, 651, 1456, 1580, 2102, 2721, 3509, 3564
BmcAI AGTACT 1 cut(s) 3439
Bme1390I CCNGG 1 cut(s) 1589
Bme18I GGWCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
BmiI GGNNCC 8 cut(s) 132, 225, 396, 1115, 2586, 2812, 2856, 3164
BmrFI CCNGG 1 cut(s) 1589
BmrI ACTGGG 3 cut(s) 1970, 2335, 2808
BmuI ACTGGG 3 cut(s) 1970, 2335, 2808
BoxI GACNNNNGTC 1 cut(s) 1694
BpiI GAAGAC 3 cut(s) 1379, 1500, 1620
BpmI CTGGAG 1 cut(s) 3510
Bpu10I CCTNAGC 3 cut(s) 2400, 2963, 3531
Bpu1102I GCTNAGC 1 cut(s) 1458
Bpu14I TTCGAA 1 cut(s) 1512
BpuEI CTTGAG 1 cut(s) 2171
BpuMI CCSGG 1 cut(s) 1589
BsaI GGTCTC 1 cut(s) 2550
BsaJI CCNNGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
BsaWI WCCGGW 2 cut(s) 202, 2559
Bsc4I CCNNNNNNNGG 4 cut(s) 952, 1588, 2220, 3325
Bse118I RCCGGY 1 cut(s) 2559
Bse1I ACTGG 9 cut(s) 123, 681, 1072, 1391, 1976, 2035, 2330, 2814, 3445
Bse3DI GCAATG 2 cut(s) 1256, 2687
BseDI CCNNGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
BseLI CCNNNNNNNGG 4 cut(s) 952, 1588, 2220, 3325
BseMI GCAATG 2 cut(s) 1256, 2687
BseMII CTCAG 5 cut(s) 272, 1076, 1449, 2391, 3096
BseNI ACTGG 9 cut(s) 123, 681, 1072, 1391, 1976, 2035, 2330, 2814, 3445
BseRI GAGGAG 1 cut(s) 2402
BseXI GCAGC 8 cut(s) 263, 661, 1441, 1565, 2087, 2706, 3494, 3574
BsgI GTGCAG 2 cut(s) 315, 775
BshFI GGCC 2 cut(s) 122, 1840
BshTI ACCGGT 1 cut(s) 2559
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 3 cut(s) 203, 1589, 2560
BsiWI CGTACG 1 cut(s) 305
BslFI GGGAC 4 cut(s) 209, 601, 2788, 2796
BslI CCNNNNNNNGG 4 cut(s) 952, 1588, 2220, 3325
BsmAI GTCTC 9 cut(s) 1274, 1479, 1671, 1707, 2550, 2955, 3025, 3581, 3594
BsmBI CGTCTC 1 cut(s) 1274
BsmFI GGGAC 4 cut(s) 209, 601, 2788, 2796
BsmI GAATGC 3 cut(s) 1263, 1520, 3007
BsnI GGCC 2 cut(s) 122, 1840
Bso31I GGTCTC 1 cut(s) 2550
Bsp119I TTCGAA 1 cut(s) 1512
Bsp1286I GDGCHC 2 cut(s) 9, 397
Bsp1407I TGTACA 1 cut(s) 2301
Bsp143I GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
Bsp1720I GCTNAGC 1 cut(s) 1458
Bsp19I CCATGG 5 cut(s) 75, 177, 834, 918, 2664
BspACI CCGC 3 cut(s) 574, 2321, 2678
BspANI GGCC 2 cut(s) 122, 1840
BspCNI CTCAG 5 cut(s) 273, 1077, 1450, 2392, 3097
BspHI TCATGA 1 cut(s) 2989
BspLI GGNNCC 8 cut(s) 132, 225, 396, 1115, 2586, 2812, 2856, 3164
BspMAI CTGCAG 1 cut(s) 2724
BspMI ACCTGC 2 cut(s) 1354, 1844
BspPI GGATC 7 cut(s) 714, 1108, 1121, 1170, 2480, 2579, 2592
BspQI GCTCTTC 1 cut(s) 1066
BspT104I TTCGAA 1 cut(s) 1512
BspTI CTTAAG 2 cut(s) 2789, 3413
BspTNI GGTCTC 1 cut(s) 2550
BsrDI GCAATG 2 cut(s) 1256, 2687
BsrFI RCCGGY 1 cut(s) 2559
BsrGI TGTACA 1 cut(s) 2301
BsrI ACTGG 9 cut(s) 123, 681, 1072, 1391, 1976, 2035, 2330, 2814, 3445
BssAI RCCGGY 1 cut(s) 2559
BssECI CCNNGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
BssMI GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
BssT1I CCWWGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
Bst6I CTCTTC 6 cut(s) 150, 1066, 1647, 2880, 3023, 3057
BstAFI CTTAAG 2 cut(s) 2789, 3413
BstAUI TGTACA 1 cut(s) 2301
BstBI TTCGAA 1 cut(s) 1512
BstC8I GCNNGC 6 cut(s) 982, 1261, 1721, 1901, 2960, 3316
BstDSI CCRYGG 5 cut(s) 75, 177, 834, 918, 2664
BstH2I RGCGCY 1 cut(s) 857
BstHHI GCGC 1 cut(s) 856
BstKTI GATC 9 cut(s) 709, 727, 1116, 1178, 2020, 2488, 2587, 2594, 3301
BstMAI GTCTC 9 cut(s) 1274, 1479, 1671, 1707, 2550, 2955, 3025, 3581, 3594
BstMBI GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
BstMWI GCNNNNNNNGC 7 cut(s) 476, 900, 1260, 1403, 1837, 2031, 3415
BstNSI RCATGY 8 cut(s) 932, 1036, 1666, 1903, 2786, 2804, 3500, 3541
BstPAI GACNNNNGTC 1 cut(s) 1694
BstSCI CCNGG 1 cut(s) 1587
BstSFI CTRYAG 3 cut(s) 2502, 2551, 2720
BstV1I GCAGC 8 cut(s) 263, 661, 1441, 1565, 2087, 2706, 3494, 3574
BstV2I GAAGAC 3 cut(s) 1379, 1500, 1620
BstX2I RGATCY 7 cut(s) 724, 1113, 1175, 2017, 2584, 2591, 3298
BstYI RGATCY 7 cut(s) 724, 1113, 1175, 2017, 2584, 2591, 3298
BsuI GTATCC 2 cut(s) 328, 2662
BsuRI GGCC 2 cut(s) 122, 1840
BtgI CCRYGG 5 cut(s) 75, 177, 834, 918, 2664
BtrI CACGTC 1 cut(s) 127
BtsI GCAGTG 3 cut(s) 659, 873, 1866
BtsIMutI CAGTG 8 cut(s) 659, 873, 1082, 1866, 2028, 2315, 2821, 3363
BveI ACCTGC 2 cut(s) 1354, 1844
Cac8I GCNNGC 6 cut(s) 982, 1261, 1721, 1901, 2960, 3316
CciI TCATGA 1 cut(s) 2989
CfoI GCGC 1 cut(s) 856
Cfr10I RCCGGY 1 cut(s) 2559
Cfr13I GGNCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
Csp6I GTAC 8 cut(s) 306, 764, 809, 845, 889, 2302, 2725, 3438
CspAI ACCGGT 1 cut(s) 2559
CspCI CAANNNNNGTGG 2 cut(s) 903, 938
CviQI GTAC 8 cut(s) 306, 764, 809, 845, 889, 2302, 2725, 3438
DpnI GATC 9 cut(s) 708, 726, 1115, 1177, 2019, 2487, 2586, 2593, 3300
DpnII GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
DraI TTTAAA 2 cut(s) 669, 817
EaeI YGGCCR 2 cut(s) 120, 1838
Eam1104I CTCTTC 6 cut(s) 150, 1066, 1647, 2880, 3023, 3057
EarI CTCTTC 6 cut(s) 150, 1066, 1647, 2880, 3023, 3057
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
Eco24I GRGCYC 2 cut(s) 9, 397
Eco31I GGTCTC 1 cut(s) 2550
Eco32I GATATC 1 cut(s) 1603
Eco47I GGWCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 8 cut(s) 781, 869, 1512, 1643, 2936, 2993, 3047, 3117
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 1207
EcoRI GAATTC 3 cut(s) 1167, 2366, 2617
EcoRV GATATC 1 cut(s) 1603
EcoT14I CCWWGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
EcoT22I ATGCAT 4 cut(s) 518, 1520, 1764, 1901
EcoT38I GRGCYC 2 cut(s) 9, 397
ErhI CCWWGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
Esp3I CGTCTC 1 cut(s) 1274
FalI AAGNNNNNCTT 4 cut(s) 986, 1018, 1312, 1344
FaqI GGGAC 4 cut(s) 209, 601, 2788, 2796
FauNDI CATATG 1 cut(s) 2543
FblI GTMKAC 1 cut(s) 1340
Fnu4HI GCNGC 9 cut(s) 252, 575, 650, 1455, 1579, 2101, 2720, 3508, 3563
FriOI GRGCYC 2 cut(s) 9, 397
Fsp4HI GCNGC 9 cut(s) 252, 575, 650, 1455, 1579, 2101, 2720, 3508, 3563
GlaI GCGC 1 cut(s) 855
GluI GCNGC 9 cut(s) 252, 575, 650, 1455, 1579, 2101, 2720, 3508, 3563
GsuI CTGGAG 1 cut(s) 3510
HaeII RGCGCY 1 cut(s) 857
HaeIII GGCC 2 cut(s) 122, 1840
HapII CCGG 3 cut(s) 203, 1589, 2560
HhaI GCGC 1 cut(s) 856
Hin6I GCGC 1 cut(s) 854
HinP1I GCGC 1 cut(s) 854
HincII GTYRAC 4 cut(s) 1155, 1341, 2211, 2442
HindII GTYRAC 4 cut(s) 1155, 1341, 2211, 2442
HindIII AAGCTT 6 cut(s) 380, 663, 2147, 2960, 3407, 3416
HpaI GTTAAC 1 cut(s) 2442
HpaII CCGG 3 cut(s) 203, 1589, 2560
HphI GGTGA 5 cut(s) 982, 2710, 2945, 2989, 3269
Hpy166II GTNNAC 9 cut(s) 130, 889, 1155, 1341, 2211, 2442, 2688, 2701, 3361
Hpy8I GTNNAC 9 cut(s) 130, 889, 1155, 1341, 2211, 2442, 2688, 2701, 3361
Hpy99I CGWCG 3 cut(s) 20, 128, 1342
HpyAV CCTTC 9 cut(s) 805, 844, 1306, 1736, 2271, 2464, 2911, 2968, 3092
HpyCH4IV ACGT 4 cut(s) 126, 362, 1337, 1993
HpyF10VI GCNNNNNNNGC 7 cut(s) 476, 900, 1260, 1403, 1837, 2031, 3415
HpySE526I ACGT 4 cut(s) 126, 362, 1337, 1993
HspAI GCGC 1 cut(s) 854
KspAI GTTAAC 1 cut(s) 2442
Kzo9I GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
LguI GCTCTTC 1 cut(s) 1066
LmnI GCTCC 5 cut(s) 4, 259, 400, 3162, 3196
Lsp1109I GCAGC 8 cut(s) 263, 661, 1441, 1565, 2087, 2706, 3494, 3574
MaeII ACGT 4 cut(s) 126, 362, 1337, 1993
MaeIII GTNAC 5 cut(s) 871, 1947, 2117, 2451, 2967
MalI GATC 9 cut(s) 708, 726, 1115, 1177, 2019, 2487, 2586, 2593, 3300
MboI GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
MfeI CAATTG 2 cut(s) 423, 444
MflI RGATCY 7 cut(s) 724, 1113, 1175, 2017, 2584, 2591, 3298
MhlI GDGCHC 2 cut(s) 9, 397
MlsI TGGCCA 1 cut(s) 1840
MluNI TGGCCA 1 cut(s) 1840
MlyI GAGTC 5 cut(s) 107, 1664, 1709, 2644, 2957
MmeI TCCRAC 5 cut(s) 1203, 1416, 1683, 3291, 3306
Mox20I TGGCCA 1 cut(s) 1840
Mph1103I ATGCAT 4 cut(s) 518, 1520, 1764, 1901
MroXI GAANNNNTTC 2 cut(s) 2366, 2474
MscI TGGCCA 1 cut(s) 1840
MslI CAYNNNNRTG 5 cut(s) 714, 933, 2663, 3285, 3495
Msp20I TGGCCA 1 cut(s) 1840
MspA1I CMGCKG 1 cut(s) 158
MspCI CTTAAG 2 cut(s) 2789, 3413
MspI CCGG 3 cut(s) 203, 1589, 2560
MspR9I CCNGG 1 cut(s) 1589
MunI CAATTG 2 cut(s) 423, 444
Mva1269I GAATGC 3 cut(s) 1263, 1520, 3007
MwoI GCNNNNNNNGC 7 cut(s) 476, 900, 1260, 1403, 1837, 2031, 3415
NciI CCSGG 1 cut(s) 1589
NcoI CCATGG 5 cut(s) 75, 177, 834, 918, 2664
NdeI CATATG 1 cut(s) 2543
NdeII GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
NlaIV GGNNCC 8 cut(s) 132, 225, 396, 1115, 2586, 2812, 2856, 3164
NmuCI GTSAC 1 cut(s) 871
NsiI ATGCAT 4 cut(s) 518, 1520, 1764, 1901
NspI RCATGY 8 cut(s) 932, 1036, 1666, 1903, 2786, 2804, 3500, 3541
NspV TTCGAA 1 cut(s) 1512
PaeI GCATGC 1 cut(s) 1903
PagI TCATGA 1 cut(s) 2989
PciI ACATGT 4 cut(s) 928, 1032, 2800, 3537
PciSI GCTCTTC 1 cut(s) 1066
PctI GAATGC 3 cut(s) 1263, 1520, 3007
PdmI GAANNNNTTC 2 cut(s) 2366, 2474
Pfl23II CGTACG 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 1587
PinAI ACCGGT 1 cut(s) 2559
PkrI GCNGC 9 cut(s) 253, 576, 651, 1456, 1580, 2102, 2721, 3509, 3564
PleI GAGTC 5 cut(s) 106, 1663, 1708, 2644, 2956
PpsI GAGTC 5 cut(s) 106, 1663, 1708, 2644, 2956
PpuMI RGGWCCY 1 cut(s) 1207
PscI ACATGT 4 cut(s) 928, 1032, 2800, 3537
PshAI GACNNNNGTC 1 cut(s) 1694
PsiI TTATAA 1 cut(s) 1121
Psp124BI GAGCTC 1 cut(s) 9
Psp1406I AACGTT 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 1207
PspLI CGTACG 1 cut(s) 305
PspN4I GGNNCC 8 cut(s) 132, 225, 396, 1115, 2586, 2812, 2856, 3164
PspPI GGNCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
PspPPI RGGWCCY 1 cut(s) 1207
PsrI GAACNNNNNNTAC 2 cut(s) 2289, 2321
PstI CTGCAG 1 cut(s) 2724
PsuI RGATCY 7 cut(s) 724, 1113, 1175, 2017, 2584, 2591, 3298
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 8 cut(s) 307, 765, 810, 846, 890, 2303, 2726, 3439
RsaNI GTAC 8 cut(s) 306, 764, 809, 845, 889, 2302, 2725, 3438
RseI CAYNNNNRTG 5 cut(s) 714, 933, 2663, 3285, 3495
SacI GAGCTC 1 cut(s) 9
SalI GTCGAC 1 cut(s) 1339
SapI GCTCTTC 1 cut(s) 1066
SatI GCNGC 9 cut(s) 252, 575, 650, 1455, 1579, 2101, 2720, 3508, 3563
Sau3AI GATC 9 cut(s) 706, 724, 1113, 1175, 2017, 2485, 2584, 2591, 3298
Sau96I GGNCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
ScaI AGTACT 1 cut(s) 3439
SchI GAGTC 5 cut(s) 107, 1664, 1709, 2644, 2957
ScrFI CCNGG 1 cut(s) 1589
SduI GDGCHC 2 cut(s) 9, 397
SfcI CTRYAG 3 cut(s) 2502, 2551, 2720
SfuI TTCGAA 1 cut(s) 1512
SinI GGWCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
SmiMI CAYNNNNRTG 5 cut(s) 714, 933, 2663, 3285, 3495
SmlI CTYRAG 3 cut(s) 2150, 2789, 3413
SmoI CTYRAG 3 cut(s) 2150, 2789, 3413
SpeI ACTAGT 2 cut(s) 2237, 3452
SphI GCATGC 1 cut(s) 1903
SsiI CCGC 3 cut(s) 574, 2321, 2678
SspI AATATT 3 cut(s) 1012, 1960, 2765
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 1587
StyI CCWWGG 9 cut(s) 75, 177, 257, 834, 918, 1203, 1841, 2664, 2770
TaiI ACGT 4 cut(s) 129, 365, 1340, 1996
TatI WGTACW 4 cut(s) 844, 2301, 2724, 3437
TauI GCSGC 1 cut(s) 577
TscAI CASTG 8 cut(s) 659, 880, 1089, 1873, 2035, 2315, 2821, 3363
TseFI GTSAC 1 cut(s) 871
TseI GCWGC 8 cut(s) 251, 649, 1454, 1578, 2100, 2719, 3507, 3562
Tsp45I GTSAC 1 cut(s) 871
TspGWI ACGGA 3 cut(s) 4, 953, 2726
TspRI CASTG 8 cut(s) 659, 880, 1089, 1873, 2035, 2315, 2821, 3363
Vha464I CTTAAG 2 cut(s) 2789, 3413
VpaK11BI GGWCC 6 cut(s) 130, 223, 1207, 2810, 3185, 3593
XapI RAATTY 8 cut(s) 436, 1167, 1324, 1791, 2366, 2617, 2878, 3040
XbaI TCTAGA 2 cut(s) 1996, 2089
XceI RCATGY 8 cut(s) 932, 1036, 1666, 1903, 2786, 2804, 3500, 3541
XcmI CCANNNNNNNNNTGG 2 cut(s) 832, 2821
XmiI GTMKAC 1 cut(s) 1340
XmnI GAANNNNTTC 2 cut(s) 2366, 2474
ZrmI AGTACT 1 cut(s) 3439
Zsp2I ATGCAT 4 cut(s) 518, 1520, 1764, 1901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.