FvH4_4g02000

Pet100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010124
Physical Location & Seq
Reverse (-)
1778087 .. 1781489
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02000.t1

Sequence Viewer

Length: 339 bp
ATGTCTAATGTGGGAACTTCAAAGGGAGTCTTGGAGATCGCTAAGTTTGGGCTTTACGTTGCCATTCCTATTGTCCTGATGTACACTTTCGCCAACAACACGTTCGTATATAGTATATCCACCAGAGGCGCCGAGGCCTCCTTCTCCAGAAGAAATGAGGGAGATGGCACGGGAACTAGCTCGCAAGAACAATGCCGGTTGATGCTTTCTTGGGGTTGTAAGATCTCCAGAATCGGTCATTTGGTCTGGGGCAGCTTTGATTGCTTAAGAGCAGTTTGCTTCCAAAGGGTAGATGCTAAATGGTCTGTAGGAGATCAATTAATTAAGGCAAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pet100

Protein Analysis

113

Amino Acids

12.38

Weight (kDa)

9.3

Isoelectric Point (pI)

25.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pet100 PF09803 10 - 35 1.7e-06 Pet100
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52825 AT1G52825 AT4G14615
fragaria_vesca FvH4_4g02000
malus_domestica MD13G1203300.v1.1 MD16G1204700.v1.1
prunus_persica Prupe.1G025800_v2.0.a1
pyrus_communis pycom13g17660
rosa_chinensis RchiOBHm_Chr4g0388961
rosa_laevigata RLG00000010003
rosa_multiflora Rmu_sc0001395.1_g000011 Rmu_sc0013650.1_g000001
rosa_roxburghii Rroxscaffold_5G00335280
rosa_rugosa Rorug03G0321700
rosa_samantha Rh4AG026200 Rh4BG019400 Rh4CG029200 Rh4DG019900
rosa_wichuraiana Rw0G003680 Rw4G001890 Rw4G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 1 cut(s) 83
AflII CTTAAG 1 cut(s) 265
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AluBI AGCT 2 cut(s) 180, 255
AluI AGCT 2 cut(s) 180, 255
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 1 cut(s) 252
ApoI RAATTY 1 cut(s) 333
AseI ATTAAT 1 cut(s) 320
AspLEI GCGC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 128
BbvI GCAGC 1 cut(s) 264
BccI CCATC 1 cut(s) 158
BfaI CTAG 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 132
BfrI CTTAAG 1 cut(s) 265
BglII AGATCT 1 cut(s) 222
BisI GCNGC 1 cut(s) 253
BlsI GCNGC 1 cut(s) 254
BmiI GGNNCC 1 cut(s) 130
BmsI GCATC 2 cut(s) 192, 283
BpmI CTGGAG 2 cut(s) 130, 211
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 132
Bse118I RCCGGY 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 264
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 196
BsnI GGCC 1 cut(s) 137
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 3 cut(s) 36, 222, 313
BspANI GGCC 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 130
BspT107I GGYRCC 1 cut(s) 128
BspTI CTTAAG 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 195
BsrGI TGTACA 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 3 cut(s) 36, 222, 313
BssNI GRCGYC 1 cut(s) 129
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 265
BstAUI TGTACA 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 1 cut(s) 42
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 3 cut(s) 39, 225, 316
BstMBI GATC 3 cut(s) 36, 222, 313
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuRI GGCC 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 182
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 195
Csp6I GTAC 1 cut(s) 82
CviJI RGCY 4 cut(s) 52, 137, 180, 255
CviKI_1 RGCY 4 cut(s) 52, 137, 180, 255
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 1 cut(s) 42
DinI GGCGCC 1 cut(s) 130
DpnI GATC 3 cut(s) 38, 224, 315
DpnII GATC 3 cut(s) 36, 222, 313
Eco147I AGGCCT 1 cut(s) 137
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
FaiI YATR 3 cut(s) 109, 111, 116
FalI AAGNNNNNCTT 2 cut(s) 14, 46
Fnu4HI GCNGC 1 cut(s) 253
Fsp4HI GCNGC 1 cut(s) 253
FspBI CTAG 1 cut(s) 177
GlaI GCGC 1 cut(s) 130
GluI GCNGC 1 cut(s) 253
GsuI CTGGAG 2 cut(s) 130, 211
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 196
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 2 cut(s) 27, 231
HpaII CCGG 1 cut(s) 196
Hpy166II GTNNAC 1 cut(s) 84
Hpy188III TCNNGA 3 cut(s) 76, 147, 228
Hpy8I GTNNAC 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 151
HpyCH4IV ACGT 2 cut(s) 57, 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 2 cut(s) 57, 101
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 1 cut(s) 129
KasI GGCGCC 1 cut(s) 128
Kzo9I GATC 3 cut(s) 36, 222, 313
LpnPI CCDG 6 cut(s) 89, 136, 160, 209, 232, 241
Lsp1109I GCAGC 1 cut(s) 264
LweI GCATC 2 cut(s) 192, 283
MaeI CTAG 1 cut(s) 177
MaeII ACGT 2 cut(s) 57, 101
MalI GATC 3 cut(s) 38, 224, 315
MboI GATC 3 cut(s) 36, 222, 313
MboII GAAGA 1 cut(s) 162
MflI RGATCY 1 cut(s) 222
MluCI AATT 3 cut(s) 317, 321, 333
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 1 cut(s) 36
MnlI CCTC 4 cut(s) 119, 127, 148, 151
MseI TTAA 3 cut(s) 266, 320, 324
MspCI CTTAAG 1 cut(s) 265
MspI CCGG 1 cut(s) 196
MwoI GCNNNNNNNGC 1 cut(s) 261
NarI GGCGCC 1 cut(s) 129
NdeII GATC 3 cut(s) 36, 222, 313
NlaIV GGNNCC 1 cut(s) 130
NmeAIII GCCGAG 1 cut(s) 157
PacI TTAATTAA 1 cut(s) 324
PceI AGGCCT 1 cut(s) 137
PfeI GAWTC 1 cut(s) 231
PkrI GCNGC 1 cut(s) 254
PleI GAGTC 1 cut(s) 35
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 35
PshBI ATTAAT 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 130
PsuI RGATCY 1 cut(s) 222
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SaqAI TTAA 3 cut(s) 266, 320, 324
SatI GCNGC 1 cut(s) 253
Sau3AI GATC 3 cut(s) 36, 222, 313
SchI GAGTC 1 cut(s) 36
SetI ASST 4 cut(s) 60, 104, 182, 257
SfaNI GCATC 2 cut(s) 192, 283
SfcI CTRYAG 1 cut(s) 306
SfoI GGCGCC 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 3 cut(s) 317, 321, 333
SseBI AGGCCT 1 cut(s) 137
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 1 cut(s) 177
StuI AGGCCT 1 cut(s) 137
TaiI ACGT 2 cut(s) 60, 104
TaqII GACCGA 1 cut(s) 224
TasI AATT 3 cut(s) 317, 321, 333
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 3 cut(s) 266, 320, 324
Tru9I TTAA 3 cut(s) 266, 320, 324
TseI GCWGC 1 cut(s) 252
Vha464I CTTAAG 1 cut(s) 265
VspI ATTAAT 1 cut(s) 320
XapI RAATTY 1 cut(s) 333
XspI CTAG 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.