RchiOBHm_Chr4g0388961

Pet100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010124
Physical Location & Seq
Reverse (-)
4153704 .. 4155106
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36213

Sequence Viewer

Length: 231 bp
ATGTCGAATATCGGAACTTCAAAGGGAGTACTGGAGATTGCCAAGTTCGGGCTCTACGTCTCCATCCCCATTGTTCTTATGTACACTTTTGCTAACAACAGTAAGAATATCCAGAGATTCATGGGAAATCGTTCTTATGTAGTATATCCACCAGAGGCGCCGAGGCCTCCGTCTCCAGAGGAAATGAGGGAGATGGCACGGGAACTAGCTAGGAAGAACAATTCGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pet100

Protein Analysis

76

Amino Acids

8.66

Weight (kDa)

9.99

Isoelectric Point (pI)

64.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pet100 PF09803 10 - 74 1.5e-10 Pet100
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52825 AT1G52825 AT4G14615
fragaria_vesca FvH4_4g02000
malus_domestica MD13G1203300.v1.1 MD16G1204700.v1.1
prunus_persica Prupe.1G025800_v2.0.a1
pyrus_communis pycom13g17660
rosa_chinensis RchiOBHm_Chr4g0388961
rosa_laevigata RLG00000010003
rosa_multiflora Rmu_sc0001395.1_g000011 Rmu_sc0013650.1_g000001
rosa_roxburghii Rroxscaffold_5G00335280
rosa_rugosa Rorug03G0321700
rosa_samantha Rh4AG026200 Rh4BG019400 Rh4CG029200 Rh4DG019900
rosa_wichuraiana Rw0G003680 Rw4G001890 Rw4G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccII CGCG 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 2 cut(s) 30, 83
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 1 cut(s) 21
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw26I GTCTC 2 cut(s) 64, 177
AoxI GGCC 1 cut(s) 164
Asp700I GAANNNNTTC 1 cut(s) 130
AspLEI GCGC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 54
BccI CCATC 2 cut(s) 71, 187
BcoDI GTCTC 2 cut(s) 64, 177
BfaI CTAG 2 cut(s) 206, 210
BfoI RGCGCY 1 cut(s) 161
BmcAI AGTACT 1 cut(s) 30
BmiI GGNNCC 1 cut(s) 159
BpmI CTGGAG 2 cut(s) 53, 159
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 161
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 226
BshFI GGCC 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 157
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 2 cut(s) 64, 177
BsmBI CGTCTC 2 cut(s) 64, 177
BsnI GGCC 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 54
Bsp1407I TGTACA 1 cut(s) 81
BspANI GGCC 1 cut(s) 166
BspFNI CGCG 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 159
BspT107I GGYRCC 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 1 cut(s) 161
BssNI GRCGYC 1 cut(s) 158
Bst4CI ACNGT 1 cut(s) 101
BstACI GRCGYC 1 cut(s) 158
BstAUI TGTACA 1 cut(s) 81
BstF5I GGATG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 226
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstMAI GTCTC 2 cut(s) 64, 177
BstUI CGCG 1 cut(s) 226
BsuRI GGCC 1 cut(s) 166
BtsCI GGATG 1 cut(s) 63
CfoI GCGC 1 cut(s) 160
Csp6I GTAC 2 cut(s) 29, 82
CviAII CATG 1 cut(s) 121
CviJI RGCY 3 cut(s) 52, 166, 209
CviKI_1 RGCY 3 cut(s) 52, 166, 209
CviQI GTAC 2 cut(s) 29, 82
DinI GGCGCC 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 54
EgeI GGCGCC 1 cut(s) 159
EheI GGCGCC 1 cut(s) 159
Esp3I CGTCTC 2 cut(s) 64, 177
FaeI CATG 1 cut(s) 124
FaiI YATR 4 cut(s) 80, 122, 138, 145
FatI CATG 1 cut(s) 120
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 1 cut(s) 54
FspBI CTAG 2 cut(s) 206, 210
GlaI GCGC 1 cut(s) 159
GsuI CTGGAG 2 cut(s) 53, 159
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 1 cut(s) 166
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 1 cut(s) 124
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 2 cut(s) 112, 176
Hpy8I GTNNAC 1 cut(s) 84
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 1 cut(s) 124
HspAI GCGC 1 cut(s) 158
KasI GGCGCC 1 cut(s) 157
LpnPI CCDG 4 cut(s) 17, 125, 165, 189
MaeI CTAG 2 cut(s) 206, 210
MaeII ACGT 1 cut(s) 57
MboII GAAGA 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 1 cut(s) 220
Mly113I GGCGCC 1 cut(s) 158
MnlI CCTC 5 cut(s) 148, 156, 172, 177, 180
MroXI GAANNNNTTC 1 cut(s) 130
MvnI CGCG 1 cut(s) 226
NarI GGCGCC 1 cut(s) 158
NlaIII CATG 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 159
NmeAIII GCCGAG 1 cut(s) 186
PceI AGGCCT 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 117
PluTI GGCGCC 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 159
RsaI GTAC 2 cut(s) 30, 83
RsaNI GTAC 2 cut(s) 29, 82
ScaI AGTACT 1 cut(s) 30
SduI GDGCHC 1 cut(s) 54
SetI ASST 2 cut(s) 60, 211
SfoI GGCGCC 1 cut(s) 159
Sse9I AATT 1 cut(s) 220
SseBI AGGCCT 1 cut(s) 166
SspDI GGCGCC 1 cut(s) 157
SspMI CTAG 2 cut(s) 206, 210
StuI AGGCCT 1 cut(s) 166
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 5
TasI AATT 1 cut(s) 220
TatI WGTACW 2 cut(s) 28, 81
TfiI GAWTC 1 cut(s) 117
TspDTI ATGAA 1 cut(s) 109
TspGWI ACGGA 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 130
XspI CTAG 2 cut(s) 206, 210
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.