MD13G1203300.v1.1

Pet100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010124
Physical Location & Seq
Reverse (-)
18298339 .. 18299438
1100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1203300.v1.1.491

Sequence Viewer

Length: 231 bp
ATGTCGAATGTGGGAACCGCGAAGGGAATTCTGGAGATGGGTAAATTTGGCCTGTACGTCTCCATCCCCATCGTTCTTATGTACACCTTCGCCAATAACTCCAAGAATCTCCAGAGATTCATGGGCAATCATTCGTATGTAGTATATCCACCAGAGGCACCGAGACCTCCATCACCGGAGGAAATGAGGGAGATGGCACGAGAGCTAGCTCGGAACAAGGCTGGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pet100

Protein Analysis

77

Amino Acids

8.56

Weight (kDa)

9.77

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pet100 PF09803 9 - 73 9e-10 Pet100
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52825 AT1G52825 AT4G14615
fragaria_vesca FvH4_4g02000
malus_domestica MD13G1203300.v1.1 MD16G1204700.v1.1
prunus_persica Prupe.1G025800_v2.0.a1
pyrus_communis pycom13g17660
rosa_chinensis RchiOBHm_Chr4g0388961
rosa_laevigata RLG00000010003
rosa_multiflora Rmu_sc0001395.1_g000011 Rmu_sc0013650.1_g000001
rosa_roxburghii Rroxscaffold_5G00335280
rosa_rugosa Rorug03G0321700
rosa_samantha Rh4AG026200 Rh4BG019400 Rh4CG029200 Rh4DG019900
rosa_wichuraiana Rw0G003680 Rw4G001890 Rw4G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccII CGCG 1 cut(s) 20
AciI CCGC 1 cut(s) 18
AcsI RAATTY 2 cut(s) 27, 44
AfaI GTAC 2 cut(s) 56, 83
AluBI AGCT 2 cut(s) 205, 209
AluI AGCT 2 cut(s) 205, 209
Alw26I GTCTC 2 cut(s) 64, 157
AoxI GGCC 1 cut(s) 49
ApoI RAATTY 2 cut(s) 27, 44
AsuHPI GGTGA 1 cut(s) 165
AsuNHI GCTAGC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 157
BauI CACGAG 1 cut(s) 198
BccI CCATC 5 cut(s) 31, 71, 77, 178, 187
BcoDI GTCTC 2 cut(s) 64, 157
BfaI CTAG 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 16, 159
BmtI GCTAGC 1 cut(s) 209
BpmI CTGGAG 2 cut(s) 53, 95
BsaI GGTCTC 1 cut(s) 157
BsaWI WCCGGW 1 cut(s) 175
BseGI GGATG 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 157
BsiSI CCGG 1 cut(s) 176
BsmAI GTCTC 2 cut(s) 64, 157
BsmBI CGTCTC 1 cut(s) 64
BsnI GGCC 1 cut(s) 51
Bso31I GGTCTC 1 cut(s) 157
Bsp1407I TGTACA 1 cut(s) 81
BspACI CCGC 1 cut(s) 18
BspANI GGCC 1 cut(s) 51
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 2 cut(s) 16, 159
BspOI GCTAGC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 157
BspTNI GGTCTC 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 81
BssSI CACGAG 1 cut(s) 198
Bst2BI CACGAG 1 cut(s) 198
BstAUI TGTACA 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 207
BstF5I GGATG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 20
BstMAI GTCTC 2 cut(s) 64, 157
BstUI CGCG 1 cut(s) 20
BsuRI GGCC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 207
Csp6I GTAC 2 cut(s) 55, 82
CviAII CATG 1 cut(s) 121
CviJI RGCY 4 cut(s) 51, 205, 209, 221
CviKI_1 RGCY 4 cut(s) 51, 205, 209, 221
CviQI GTAC 2 cut(s) 55, 82
Eco31I GGTCTC 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 124
FaiI YATR 4 cut(s) 80, 122, 138, 145
FatI CATG 1 cut(s) 120
FokI GGATG 1 cut(s) 50
FspBI CTAG 1 cut(s) 206
GsaI CCCAGC 1 cut(s) 225
GsuI CTGGAG 2 cut(s) 53, 95
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 176
Hin1II CATG 1 cut(s) 124
HinfI GANTC 2 cut(s) 106, 117
HpaII CCGG 1 cut(s) 176
HphI GGTGA 1 cut(s) 165
Hpy166II GTNNAC 1 cut(s) 84
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 2 cut(s) 32, 112
Hpy8I GTNNAC 1 cut(s) 84
HpyAV CCTTC 2 cut(s) 16, 97
HpyCH4IV ACGT 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 1 cut(s) 124
LpnPI CCDG 6 cut(s) 17, 65, 125, 165, 189, 207
MaeI CTAG 1 cut(s) 206
MaeII ACGT 1 cut(s) 57
MluCI AATT 2 cut(s) 27, 44
MnlI CCTC 4 cut(s) 148, 172, 177, 180
MslI CAYNNNNRTG 1 cut(s) 135
MspI CCGG 1 cut(s) 176
MvnI CGCG 1 cut(s) 20
NheI GCTAGC 1 cut(s) 205
NlaIII CATG 1 cut(s) 124
NlaIV GGNNCC 2 cut(s) 16, 159
PfeI GAWTC 2 cut(s) 106, 117
PspFI CCCAGC 1 cut(s) 221
PspN4I GGNNCC 2 cut(s) 16, 159
RsaI GTAC 2 cut(s) 56, 83
RsaNI GTAC 2 cut(s) 55, 82
RseI CAYNNNNRTG 1 cut(s) 135
SetI ASST 5 cut(s) 60, 89, 169, 207, 211
SmiMI CAYNNNNRTG 1 cut(s) 135
Sse9I AATT 2 cut(s) 27, 44
SsiI CCGC 1 cut(s) 18
SspMI CTAG 1 cut(s) 206
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 5
TasI AATT 2 cut(s) 27, 44
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 2 cut(s) 106, 117
TspDTI ATGAA 1 cut(s) 109
XapI RAATTY 2 cut(s) 27, 44
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.