Rorug03G0321700

Pet100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010124
Physical Location & Seq
Reverse (-)
34903171 .. 34904611
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0321700.1

Sequence Viewer

Length: 498 bp
ATGGAGAACATGTTGGGTCTGTTGAGAATTCATATCCAAAGAGGAGTGAACCTTGCCGTCAGAGACATGAGAAGCAGCGACCCTTATCTCATTGTCAGAATGGGCAAGCAGAAGCTAAAGACTCGTGTAGTGAAGAAGAGCGTGAATCCTGAGTGGAATGAACAACTGACTCTTTCAATTGCTGATCCAAATCTTCCAATCCTGGTCTCTGTGTATGACAAGGATACATTTAGTTTTGATGACAAAATGGGGGATGCAGAGTTTGAGATTGGTCCATTCATTAAAGCCTTGAAGATGGGTTTCCAAGGCCTTGAAGATGGAGCCATAATTTCTAAAGTGCAGGTGAACAAGCAAAACTGTCTAGCTGAAGAGAGCTCCATTATTTACTCCGAAGGCAAACTTGTACAGAACATGGTTCTCAGACTCAGAAATGTCGAGTGCGGGGAAGTGGAACTCCAATTGCAGTGGATTGATGTACCTAGTTCTAGGGGTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pet100

Protein Analysis

165

Amino Acids

18.68

Weight (kDa)

5.91

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 92 2.9e-22 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52825 AT1G52825 AT4G14615
fragaria_vesca FvH4_4g02000
malus_domestica MD13G1203300.v1.1 MD16G1204700.v1.1
prunus_persica Prupe.1G025800_v2.0.a1
pyrus_communis pycom13g17660
rosa_chinensis RchiOBHm_Chr4g0388961
rosa_laevigata RLG00000010003
rosa_multiflora Rmu_sc0001395.1_g000011 Rmu_sc0013650.1_g000001
rosa_roxburghii Rroxscaffold_5G00335280
rosa_rugosa Rorug03G0321700
rosa_samantha Rh4AG026200 Rh4BG019400 Rh4CG029200 Rh4DG019900
rosa_wichuraiana Rw0G003680 Rw4G001890 Rw4G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 331
Acc36I ACCTGC 1 cut(s) 331
AciI CCGC 1 cut(s) 441
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 2 cut(s) 405, 477
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 3 cut(s) 177, 292, 314
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 3 cut(s) 115, 365, 375
AluI AGCT 3 cut(s) 115, 365, 375
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 2 cut(s) 57, 211
AlwI GGATC 1 cut(s) 179
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 75
ApoI RAATTY 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 355
AvaII GGWCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 377
BauI CACGAG 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 1 cut(s) 87
BccI CCATC 2 cut(s) 289, 311
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 203
BciVI GTATCC 1 cut(s) 217
BcoDI GTCTC 2 cut(s) 57, 211
BfaI CTAG 3 cut(s) 362, 480, 486
BfmI CTRYAG 1 cut(s) 494
BfuAI ACCTGC 1 cut(s) 331
BfuI GTATCC 1 cut(s) 217
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 304
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 259
BseJI GATNNNNATC 1 cut(s) 189
BseMII CTCAG 3 cut(s) 141, 433, 439
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 1 cut(s) 87
BsgI GTGCAG 1 cut(s) 359
BshFI GGCC 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 377
BsmAI GTCTC 2 cut(s) 57, 211
BsnI GGCC 1 cut(s) 309
Bso31I GGTCTC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1407I TGTACA 1 cut(s) 403
Bsp143I GATC 1 cut(s) 184
BspACI CCGC 1 cut(s) 441
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 3 cut(s) 142, 432, 438
BspLI GGNNCC 1 cut(s) 322
BspMI ACCTGC 1 cut(s) 331
BspPI GGATC 1 cut(s) 179
BspQI GCTCTTC 1 cut(s) 131
BspTNI GGTCTC 1 cut(s) 211
BsrGI TGTACA 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 1 cut(s) 184
BssSI CACGAG 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 304
Bst2BI CACGAG 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 2 cut(s) 131, 363
BstAUI TGTACA 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 3 cut(s) 150, 419, 425
BstF5I GGATG 1 cut(s) 259
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 2 cut(s) 57, 211
BstMBI GATC 1 cut(s) 184
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 87
BsuI GTATCC 1 cut(s) 217
BsuRI GGCC 1 cut(s) 309
BtsCI GGATG 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 1 cut(s) 470
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 272
Csp6I GTAC 2 cut(s) 404, 476
CspCI CAANNNNNGTGG 2 cut(s) 446, 481
CviAII CATG 3 cut(s) 10, 67, 412
CviJI RGCY 6 cut(s) 115, 287, 309, 323, 365, 375
CviKI_1 RGCY 6 cut(s) 115, 287, 309, 323, 365, 375
CviQI GTAC 2 cut(s) 404, 476
DdeI CTNAG 3 cut(s) 150, 419, 425
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 131, 363
EarI CTCTTC 2 cut(s) 131, 363
Ecl136II GAGCTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 304
Eco147I AGGCCT 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 377
Eco31I GGTCTC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 272
Eco53kI GAGCTC 1 cut(s) 375
Eco57I CTGAAG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 3 cut(s) 13, 70, 415
FaiI YATR 7 cut(s) 11, 33, 68, 216, 326, 413, 496
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 3 cut(s) 9, 66, 411
FauI CCCGC 1 cut(s) 434
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 3 cut(s) 362, 480, 486
GluI GCNGC 1 cut(s) 76
HaeIII GGCC 1 cut(s) 309
Hin1II CATG 3 cut(s) 13, 70, 415
HinfI GANTC 4 cut(s) 121, 145, 169, 423
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 2 cut(s) 49, 346
Hpy188I TCNGA 5 cut(s) 62, 98, 391, 422, 428
Hpy188III TCNNGA 1 cut(s) 149
Hpy8I GTNNAC 2 cut(s) 49, 346
HpyAV CCTTC 1 cut(s) 386
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4V TGCA 3 cut(s) 257, 340, 463
HpyF3I CTNAG 3 cut(s) 150, 419, 425
Hsp92II CATG 3 cut(s) 13, 70, 415
Kzo9I GATC 1 cut(s) 184
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 2 cut(s) 320, 380
LpnPI CCDG 4 cut(s) 162, 188, 215, 326
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 244
MaeI CTAG 3 cut(s) 362, 480, 486
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 6 cut(s) 145, 148, 185, 304, 326, 380
MfeI CAATTG 2 cut(s) 177, 458
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 4 cut(s) 27, 177, 327, 458
MlyI GAGTC 3 cut(s) 115, 163, 417
MnlI CCTC 1 cut(s) 35
MseI TTAA 1 cut(s) 282
MspR9I CCNGG 1 cut(s) 203
MunI CAATTG 2 cut(s) 177, 458
MvaI CCWGG 1 cut(s) 203
NdeII GATC 1 cut(s) 184
NlaIII CATG 3 cut(s) 13, 70, 415
NlaIV GGNNCC 1 cut(s) 322
NspI RCATGY 1 cut(s) 13
PaqCI CACCTGC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 309
PciI ACATGT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 131
PfeI GAWTC 1 cut(s) 145
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 3 cut(s) 115, 163, 417
PpsI GAGTC 3 cut(s) 115, 163, 417
PscI ACATGT 1 cut(s) 9
Psp124BI GAGCTC 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 272
RsaI GTAC 2 cut(s) 405, 477
RsaNI GTAC 2 cut(s) 404, 476
SacI GAGCTC 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 3 cut(s) 115, 163, 417
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 377
SetI ASST 6 cut(s) 54, 117, 345, 367, 377, 481
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 494
SinI GGWCC 1 cut(s) 272
Sse9I AATT 4 cut(s) 27, 177, 327, 458
SseBI AGGCCT 1 cut(s) 309
SsiI CCGC 1 cut(s) 441
SspMI CTAG 3 cut(s) 362, 480, 486
SstI GAGCTC 1 cut(s) 377
StuI AGGCCT 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 359
TaqI TCGA 1 cut(s) 435
TasI AATT 4 cut(s) 27, 177, 327, 458
TatI WGTACW 1 cut(s) 403
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 470
TseI GCWGC 1 cut(s) 75
TspDTI ATGAA 3 cut(s) 20, 174, 268
TspRI CASTG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 272
XapI RAATTY 1 cut(s) 27
XceI RCATGY 1 cut(s) 13
XspI CTAG 3 cut(s) 362, 480, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.