Rh4DG019900

Pet100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010124
Physical Location & Seq
Reverse (-)
3065454 .. 3066596
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG019900.1

Sequence Viewer

Length: 309 bp
ATGAAGCTTGAAGACTATAGGCTTCTTCTTCAATATTTCTGGAATCCGCTCCCTAAAATCCTCGAGCTTCTGGTGAAAATGTCGAATATCGGAACTTCAAAGGGAGTACTGGAGATTGCAAAGTTCGGGCTCTACGTCTCCATCCCCATTGTTCTTATGTACACTTTTGCTAACAACAGCAAGAACATCCAGAGATTCATGGGAAATCGTTCTTATGTAGTATATCCACCAGAGGCGCCGAGGCCTCCGTCTCCAGAGGAAATGAGGGAGATGGCACGGGAACTAGCTAGGAAGAACAATTCGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pet100

Protein Analysis

102

Amino Acids

11.92

Weight (kDa)

9.79

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pet100 PF09803 36 - 100 4e-10 Pet100
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52825 AT1G52825 AT4G14615
fragaria_vesca FvH4_4g02000
malus_domestica MD13G1203300.v1.1 MD16G1204700.v1.1
prunus_persica Prupe.1G025800_v2.0.a1
pyrus_communis pycom13g17660
rosa_chinensis RchiOBHm_Chr4g0388961
rosa_laevigata RLG00000010003
rosa_multiflora Rmu_sc0001395.1_g000011 Rmu_sc0013650.1_g000001
rosa_roxburghii Rroxscaffold_5G00335280
rosa_rugosa Rorug03G0321700
rosa_samantha Rh4AG026200 Rh4BG019400 Rh4CG029200 Rh4DG019900
rosa_wichuraiana Rw0G003680 Rw4G001890 Rw4G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccBSI CCGCTC 1 cut(s) 49
AccII CGCG 1 cut(s) 304
AciI CCGC 1 cut(s) 47
AcyI GRCGYC 1 cut(s) 236
AfaI GTAC 2 cut(s) 108, 161
AgsI TTSAA 3 cut(s) 11, 32, 99
AluBI AGCT 3 cut(s) 7, 67, 287
AluI AGCT 3 cut(s) 7, 67, 287
Alw26I GTCTC 2 cut(s) 142, 255
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 1 cut(s) 242
Asp700I GAANNNNTTC 1 cut(s) 208
AspLEI GCGC 1 cut(s) 238
AsuHPI GGTGA 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 62
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 18
BccI CCATC 2 cut(s) 149, 265
BcoDI GTCTC 2 cut(s) 142, 255
BfaI CTAG 2 cut(s) 284, 288
BfmI CTRYAG 1 cut(s) 16
BfoI RGCGCY 1 cut(s) 239
BmcAI AGTACT 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 237
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 2 cut(s) 131, 237
BsaHI GRCGYC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 239
BseGI GGATG 2 cut(s) 141, 186
BseNI ACTGG 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 235
BsiHKCI CYCGRG 1 cut(s) 62
BsmAI GTCTC 2 cut(s) 142, 255
BsmBI CGTCTC 2 cut(s) 142, 255
BsnI GGCC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 132
Bsp1407I TGTACA 1 cut(s) 159
BspACI CCGC 1 cut(s) 47
BspANI GGCC 1 cut(s) 244
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 159
BsrI ACTGG 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 239
BssNI GRCGYC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 236
BstAUI TGTACA 1 cut(s) 159
BstF5I GGATG 2 cut(s) 141, 186
BstFNI CGCG 1 cut(s) 304
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 1 cut(s) 238
BstMAI GTCTC 2 cut(s) 142, 255
BstSFI CTRYAG 1 cut(s) 16
BstUI CGCG 1 cut(s) 304
BstV2I GAAGAC 1 cut(s) 18
BsuRI GGCC 1 cut(s) 244
BtsCI GGATG 2 cut(s) 141, 186
CfoI GCGC 1 cut(s) 238
Csp6I GTAC 2 cut(s) 107, 160
CviAII CATG 1 cut(s) 199
CviJI RGCY 6 cut(s) 7, 22, 67, 130, 244, 287
CviKI_1 RGCY 6 cut(s) 7, 22, 67, 130, 244, 287
CviQI GTAC 2 cut(s) 107, 160
DinI GGCGCC 1 cut(s) 237
Eco147I AGGCCT 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 132
EgeI GGCGCC 1 cut(s) 237
EheI GGCGCC 1 cut(s) 237
Esp3I CGTCTC 2 cut(s) 142, 255
FaeI CATG 1 cut(s) 202
FaiI YATR 5 cut(s) 18, 158, 200, 216, 223
FatI CATG 1 cut(s) 198
FokI GGATG 2 cut(s) 128, 173
FriOI GRGCYC 1 cut(s) 132
FspBI CTAG 2 cut(s) 284, 288
GlaI GCGC 1 cut(s) 237
GsuI CTGGAG 2 cut(s) 131, 237
HaeII RGCGCY 1 cut(s) 239
HaeIII GGCC 1 cut(s) 244
HhaI GCGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 236
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 43, 195
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 1 cut(s) 92
Hpy188III TCNNGA 3 cut(s) 40, 190, 254
Hpy8I GTNNAC 1 cut(s) 162
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 1 cut(s) 119
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 236
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 1 cut(s) 236
KasI GGCGCC 1 cut(s) 235
LmnI GCTCC 1 cut(s) 54
LpnPI CCDG 6 cut(s) 25, 56, 95, 203, 243, 267
MaeI CTAG 2 cut(s) 284, 288
MaeII ACGT 1 cut(s) 135
MbiI CCGCTC 1 cut(s) 49
MboII GAAGA 4 cut(s) 17, 20, 23, 304
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 1 cut(s) 298
Mly113I GGCGCC 1 cut(s) 236
MnlI CCTC 6 cut(s) 71, 226, 234, 250, 255, 258
MroXI GAANNNNTTC 1 cut(s) 208
MvnI CGCG 1 cut(s) 304
NarI GGCGCC 1 cut(s) 236
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 1 cut(s) 264
PaeR7I CTCGAG 1 cut(s) 62
PceI AGGCCT 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 43, 195
PluTI GGCGCC 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 237
PspXI VCTCGAGB 1 cut(s) 62
RsaI GTAC 2 cut(s) 108, 161
RsaNI GTAC 2 cut(s) 107, 160
ScaI AGTACT 1 cut(s) 108
SduI GDGCHC 1 cut(s) 132
SetI ASST 4 cut(s) 9, 69, 138, 289
SfcI CTRYAG 1 cut(s) 16
SfoI GGCGCC 1 cut(s) 237
Sfr274I CTCGAG 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 1 cut(s) 298
SseBI AGGCCT 1 cut(s) 244
SsiI CCGC 1 cut(s) 47
SspDI GGCGCC 1 cut(s) 235
SspI AATATT 1 cut(s) 35
SspMI CTAG 2 cut(s) 284, 288
StuI AGGCCT 1 cut(s) 244
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 63, 83
TasI AATT 1 cut(s) 298
TatI WGTACW 2 cut(s) 106, 159
TfiI GAWTC 2 cut(s) 43, 195
TspDTI ATGAA 2 cut(s) 17, 187
TspGWI ACGGA 1 cut(s) 237
XhoI CTCGAG 1 cut(s) 62
XmnI GAANNNNTTC 1 cut(s) 208
XspI CTAG 2 cut(s) 284, 288
ZrmI AGTACT 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.