FvH4_4g03060

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010554
Physical Location & Seq
Reverse (-)
2755038 .. 2757039
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03060.t5

Sequence Viewer

Length: 561 bp
ATGCCACACATCATCTCTATCAATGCTGGGGAGGATGTCTGCGGGATAGTGAAGAGATTTTCTGACAAAACACCCCGAGTTATTTGCGTGATGTCTGCCAGTGGAGCAGTCTCTAATGTAAAACTTCGCCAACAGGATGGAACAGTCAACACACATGATGGCCTTTTCAATATCTTGAGCTTGTCTGGATGTCTTATTCCCCAGGGAGACTCGATTCAGGGAACAACGACCTGGATTGGTGGATTAACTGTCTCTCTAGCAGGTGTTGATGGTCGAGCTTATGGGGGGATTGTGAACGGAGCCATGAGAGCTGAAACACCTGTCATGGTTGTGGTAGGTAGTTTTCTACCTGGTGTTCAGATAGGAAAGGGTAAAAAGTCTGACGCTGGACCATCTACAGCTATGCCACCGGCGTCGCCAGCACCAGCGCCTCCGTCACCGCTGGCATCAGCGCCACCACCCCCACCGTCACCAGCACCAGGACCATCAGCATCATCTCCTGTACCAGACTGGTGGGCCGGGTGTGCAGATAGCTGGCCAAGATTTGATCCAGGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

187

Amino Acids

18.79

Weight (kDa)

6.7

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 2 - 113 1e-16 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 251
Acc36I ACCTGC 1 cut(s) 251
AciI CCGC 2 cut(s) 42, 440
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 5 cut(s) 201, 230, 349, 478, 550
AluBI AGCT 5 cut(s) 180, 278, 311, 401, 534
AluI AGCT 5 cut(s) 180, 278, 311, 401, 534
Alw26I GTCTC 3 cut(s) 115, 201, 256
AlwI GGATC 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 3 cut(s) 160, 516, 536
AspLEI GCGC 2 cut(s) 430, 454
AspS9I GGNCC 3 cut(s) 389, 482, 516
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 2 cut(s) 429, 462
AvaI CYCGRG 1 cut(s) 75
AvaII GGWCC 2 cut(s) 389, 482
BalI TGGCCA 1 cut(s) 538
BccI CCATC 5 cut(s) 131, 152, 263, 400, 493
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciT130I CCWGG 5 cut(s) 203, 232, 351, 480, 552
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 3 cut(s) 115, 201, 256
BfaI CTAG 1 cut(s) 257
BfmI CTRYAG 1 cut(s) 396
BfoI RGCGCY 2 cut(s) 431, 455
BfuAI ACCTGC 1 cut(s) 251
Bme1390I CCNGG 6 cut(s) 203, 232, 351, 480, 520, 552
Bme18I GGWCC 2 cut(s) 389, 482
BmeT110I CYCGRG 1 cut(s) 75
BmgT120I GGNCC 3 cut(s) 389, 482, 516
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 6 cut(s) 203, 232, 351, 480, 520, 552
BmsI GCATC 2 cut(s) 455, 500
BpuEI CTTGAG 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 201, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 2 cut(s) 99, 515
BseBI CCWGG 5 cut(s) 203, 232, 351, 480, 552
BseDI CCNNGG 2 cut(s) 201, 202
BseGI GGATG 3 cut(s) 40, 142, 194
BseLI CCNNNNNNNGG 1 cut(s) 479
BseNI ACTGG 2 cut(s) 99, 515
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 546
BshFI GGCC 3 cut(s) 162, 518, 538
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 2 cut(s) 410, 519
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 3 cut(s) 115, 201, 256
BsnI GGCC 3 cut(s) 162, 518, 538
BsoBI CYCGRG 1 cut(s) 75
Bsp143I GATC 2 cut(s) 547, 554
BspACI CCGC 2 cut(s) 42, 440
BspANI GGCC 3 cut(s) 162, 518, 538
BspLI GGNNCC 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 251
BspPI GGATC 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 2 cut(s) 99, 515
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 2 cut(s) 201, 202
BssMI GATC 2 cut(s) 547, 554
BssNI GRCGYC 1 cut(s) 413
Bst2UI CCWGG 5 cut(s) 203, 232, 351, 480, 552
Bst4CI ACNGT 3 cut(s) 145, 250, 468
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 420, 444, 536
BstF5I GGATG 3 cut(s) 40, 142, 194
BstH2I RGCGCY 2 cut(s) 431, 455
BstHHI GCGC 2 cut(s) 430, 454
BstKTI GATC 2 cut(s) 550, 557
BstMAI GTCTC 3 cut(s) 115, 201, 256
BstMBI GATC 2 cut(s) 547, 554
BstMWI GCNNNNNNNGC 4 cut(s) 104, 308, 419, 524
BstNI CCWGG 5 cut(s) 203, 232, 351, 480, 552
BstSCI CCNGG 6 cut(s) 201, 230, 349, 478, 518, 550
BstSFI CTRYAG 1 cut(s) 396
BstXI CCANNNNNNTGG 2 cut(s) 137, 513
BsuRI GGCC 3 cut(s) 162, 518, 538
BtsCI GGATG 3 cut(s) 40, 142, 194
BtsIMutI CAGTG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 3 cut(s) 420, 444, 536
CfoI GCGC 2 cut(s) 430, 454
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 3 cut(s) 389, 482, 516
CseI GACGC 2 cut(s) 392, 402
CsiI ACCWGGT 1 cut(s) 349
Csp6I GTAC 1 cut(s) 503
CviAII CATG 3 cut(s) 155, 304, 325
CviJI RGCY 9 cut(s) 162, 180, 278, 302, 311, 401, 518, 534, 538
CviKI_1 RGCY 9 cut(s) 162, 180, 278, 302, 311, 401, 518, 534, 538
CviQI GTAC 1 cut(s) 503
DpnI GATC 2 cut(s) 549, 556
DpnII GATC 2 cut(s) 547, 554
EaeI YGGCCR 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco47I GGWCC 2 cut(s) 389, 482
Eco88I CYCGRG 1 cut(s) 75
EcoRII CCWGG 5 cut(s) 201, 230, 349, 478, 550
FaeI CATG 3 cut(s) 158, 307, 328
FaiI YATR 6 cut(s) 156, 282, 305, 326, 404, 559
FatI CATG 3 cut(s) 154, 303, 324
FauI CCCGC 1 cut(s) 35
FokI GGATG 3 cut(s) 47, 149, 201
FspBI CTAG 1 cut(s) 257
GlaI GCGC 2 cut(s) 429, 453
GsaI CCCAGC 1 cut(s) 30
HaeII RGCGCY 2 cut(s) 431, 455
HaeIII GGCC 3 cut(s) 162, 518, 538
HapII CCGG 2 cut(s) 410, 519
HgaI GACGC 2 cut(s) 392, 402
HhaI GCGC 2 cut(s) 430, 454
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 3 cut(s) 158, 307, 328
Hin6I GCGC 2 cut(s) 428, 452
HinP1I GCGC 2 cut(s) 428, 452
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 2 cut(s) 209, 214
HpaII CCGG 2 cut(s) 410, 519
HphI GGTGA 2 cut(s) 429, 462
Hpy166II GTNNAC 2 cut(s) 148, 295
Hpy188I TCNGA 3 cut(s) 64, 360, 382
Hpy188III TCNNGA 2 cut(s) 175, 186
Hpy8I GTNNAC 2 cut(s) 148, 295
Hpy99I CGWCG 1 cut(s) 418
HpyCH4III ACNGT 3 cut(s) 145, 250, 468
HpyCH4V TGCA 1 cut(s) 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 308, 419, 524
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 3 cut(s) 158, 307, 328
HspAI GCGC 2 cut(s) 428, 452
Kzo9I GATC 2 cut(s) 547, 554
LmnI GCTCC 2 cut(s) 104, 299
LweI GCATC 2 cut(s) 455, 500
MabI ACCWGGT 1 cut(s) 349
MaeI CTAG 1 cut(s) 257
MaeIII GTNAC 2 cut(s) 435, 468
MalI GATC 2 cut(s) 549, 556
MboI GATC 2 cut(s) 547, 554
MboII GAAGA 1 cut(s) 64
MlsI TGGCCA 1 cut(s) 538
MluNI TGGCCA 1 cut(s) 538
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 2 cut(s) 25, 441
Mox20I TGGCCA 1 cut(s) 538
MscI TGGCCA 1 cut(s) 538
MseI TTAA 1 cut(s) 245
MslI CAYNNNNRTG 1 cut(s) 329
Msp20I TGGCCA 1 cut(s) 538
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 2 cut(s) 410, 519
MspR9I CCNGG 6 cut(s) 203, 232, 351, 480, 520, 552
MvaI CCWGG 5 cut(s) 203, 232, 351, 480, 552
MwoI GCNNNNNNNGC 4 cut(s) 104, 308, 419, 524
NciI CCSGG 1 cut(s) 520
NdeII GATC 2 cut(s) 547, 554
NlaIII CATG 3 cut(s) 158, 307, 328
NlaIV GGNNCC 1 cut(s) 301
NmuCI GTSAC 2 cut(s) 435, 468
PaqCI CACCTGC 1 cut(s) 251
PasI CCCWGGG 1 cut(s) 202
PfeI GAWTC 1 cut(s) 214
PfoI TCCNGGA 1 cut(s) 550
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Psp6I CCWGG 5 cut(s) 201, 230, 349, 478, 550
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 5 cut(s) 201, 230, 349, 478, 550
PspN4I GGNNCC 1 cut(s) 301
PspPI GGNCC 3 cut(s) 389, 482, 516
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 1 cut(s) 245
Sau3AI GATC 2 cut(s) 547, 554
Sau96I GGNCC 3 cut(s) 389, 482, 516
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 6 cut(s) 203, 232, 351, 480, 520, 552
SexAI ACCWGGT 1 cut(s) 349
SfaNI GCATC 2 cut(s) 455, 500
SfcI CTRYAG 1 cut(s) 396
SgrAI CRCCGGYG 1 cut(s) 409
SinI GGWCC 2 cut(s) 389, 482
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
SsiI CCGC 2 cut(s) 42, 440
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 6 cut(s) 201, 230, 349, 478, 518, 550
TaaI ACNGT 3 cut(s) 145, 250, 468
TaqI TCGA 2 cut(s) 212, 274
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 2 cut(s) 435, 468
Tsp45I GTSAC 2 cut(s) 435, 468
TspGWI ACGGA 2 cut(s) 312, 423
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 2 cut(s) 389, 482
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.