Rw4G002880

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010554
Physical Location & Seq
Reverse (-)
5739283 .. 5741627
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002880.1

Sequence Viewer

Length: 924 bp
ATGTACGCCAGAGAAGAACTGCCTGGAGAAGGGGTTGAAGTGATTGCGGCTGATGCTCCCTCGGAGTACCAGATGGCTCCTAGGGCGGTGGAGCACAAGGACAAGGCGGCTGCTGGTTCAGGAGAGGGGACGGGTGAGCCAAAGAAGAGAGGGAGAGGGAGGCCTCCACAGAAGAATGTGGGGGAAGGGCAGTCCTCCTCAGCCAAGGGGAGAAAGGAGAAGGGCCAAGGAAGCGGCGGTGGGGGTGGAGGAGGTGGAAGTGGAGGTGCCGGTGGCAGTGGCAGTGGGGGCGGAGGAGGAGGAGGAGGAGGTGGTGGGGGCGGGGGCGGCGGCCAGGGCAATCCCGGTGCAGCTTATAATGGAAGAAGCTTCACGCCCCACCTCATCTCTGTCAATGCTGGGGAGAATGTCTGCGGGAGCGTTATGAGATATTTCCATGAAAGACCACGAGCAATCTGCGTGATTTCTGCAAGCGGAGTAATCTCTAGTGTTAATCTGCGCCAACAGGATGACGAGGCCATCACATATGAGGGACGTTTCGAAATCCTGAGCTTGTCTGGATGCCTTATGCCCCATAACGGGGCCTGGCGTGGTGCAATGAGTGTCTCTCTGGCAGGCGCCAACGGTGCAATTTTAGGGGGAGGCATTAATGGAATTCTGAGAGCTGCTACTCCTGTGCAAGTTGTGGTAGGTAGTTTCCTAGCTGGTGATCCGAAAGGAAAGGGAAAGAAGAAGGGGGATGCTGAACCACCTGATACTGAACCTCCACCTCCGCCTCCCCCTCCGCCACCGCCGTCGCCTCCACCGCCAGCACCAGAACCGCCACCTGCACCAGGACCACCACCACAACCACCACCACCACCACAAGAGCCCATGGGATTCTGGGGGATATACGATAATAGCTGGCCGAGAATGGAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

307

Amino Acids

30.59

Weight (kDa)

6.92

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 126 - 233 2.2e-21 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AarI CACCTGC 1 cut(s) 835
Acc36I ACCTGC 1 cut(s) 835
AccB1I GGYRCC 2 cut(s) 266, 617
AclWI GGATC 1 cut(s) 704
AcoI YGGCCR 2 cut(s) 331, 905
AcsI RAATTY 1 cut(s) 654
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 2 cut(s) 5, 68
AfiI CCNNNNNNNGG 5 cut(s) 29, 578, 579, 580, 833
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 4 cut(s) 22, 333, 584, 832
AluBI AGCT 6 cut(s) 353, 369, 552, 665, 704, 903
AluI AGCT 6 cut(s) 353, 369, 552, 665, 704, 903
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 610
AlwI GGATC 1 cut(s) 704
AoxI GGCC 6 cut(s) 161, 223, 331, 516, 582, 905
ApeKI GCWGC 3 cut(s) 110, 350, 665
ApoI RAATTY 1 cut(s) 654
AseI ATTAAT 1 cut(s) 648
AspA2I CCTAGG 1 cut(s) 80
AspLEI GCGC 2 cut(s) 501, 620
AspS9I GGNCC 3 cut(s) 223, 582, 836
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 146, 719
AsuII TTCGAA 1 cut(s) 540
AvaII GGWCC 1 cut(s) 836
AvrII CCTAGG 1 cut(s) 80
BanI GGYRCC 2 cut(s) 266, 617
BanII GRGCYC 1 cut(s) 873
BauI CACGAG 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 199
BbvI GCAGC 3 cut(s) 97, 362, 652
BccI CCATC 2 cut(s) 67, 527
BceAI ACGGC 1 cut(s) 778
BcgI CGANNNNNNTGC 2 cut(s) 438, 472
BciT130I CCWGG 4 cut(s) 24, 335, 586, 834
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 610
BfaI CTAG 3 cut(s) 81, 486, 701
BfoI RGCGCY 1 cut(s) 621
BfuAI ACCTGC 1 cut(s) 835
BisI GCNGC 8 cut(s) 48, 108, 111, 235, 328, 331, 351, 666
BlnI CCTAGG 1 cut(s) 80
BlsI GCNGC 8 cut(s) 49, 109, 112, 236, 329, 332, 352, 667
Bme1390I CCNGG 5 cut(s) 24, 335, 345, 586, 834
Bme18I GGWCC 1 cut(s) 836
BmgT120I GGNCC 3 cut(s) 223, 582, 836
BmiI GGNNCC 4 cut(s) 78, 268, 583, 619
BmrFI CCNGG 5 cut(s) 24, 335, 345, 586, 834
BmsI GCATC 3 cut(s) 43, 551, 730
BplI GAGNNNNNCTC 2 cut(s) 592, 624
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 2 cut(s) 199, 548
Bpu14I TTCGAA 1 cut(s) 540
BpuMI CCSGG 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 6 cut(s) 60, 80, 204, 226, 334, 873
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 748, 778
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 578, 579, 580, 833
Bse118I RCCGGY 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 4 cut(s) 24, 335, 586, 834
BseDI CCNNGG 6 cut(s) 60, 80, 204, 226, 334, 873
BseGI GGATG 3 cut(s) 514, 566, 745
BseLI CCNNNNNNNGG 5 cut(s) 29, 578, 579, 580, 833
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 3 cut(s) 213, 539, 650
BseRI GAGGAG 7 cut(s) 187, 264, 309, 312, 315, 318, 321
BseXI GCAGC 3 cut(s) 97, 362, 652
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 2 cut(s) 369, 813
BshFI GGCC 6 cut(s) 163, 225, 333, 518, 584, 907
BshNI GGYRCC 2 cut(s) 266, 617
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 270, 345
BslFI GGGAC 2 cut(s) 142, 546
BslI CCNNNNNNNGG 5 cut(s) 29, 578, 579, 580, 833
BsmAI GTCTC 1 cut(s) 610
BsmFI GGGAC 2 cut(s) 142, 546
BsnI GGCC 6 cut(s) 163, 225, 333, 518, 584, 907
Bsp119I TTCGAA 1 cut(s) 540
Bsp1286I GDGCHC 2 cut(s) 96, 873
Bsp143I GATC 1 cut(s) 709
Bsp19I CCATGG 1 cut(s) 873
BspANI GGCC 6 cut(s) 163, 225, 333, 518, 584, 907
BspCNI CTCAG 3 cut(s) 212, 540, 651
BspLI GGNNCC 4 cut(s) 78, 268, 583, 619
BspMI ACCTGC 1 cut(s) 835
BspPI GGATC 1 cut(s) 704
BspT104I TTCGAA 1 cut(s) 540
BspT107I GGYRCC 2 cut(s) 266, 617
BsrDI GCAATG 1 cut(s) 603
BsrFI RCCGGY 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 6 cut(s) 60, 80, 204, 226, 334, 873
BssMI GATC 1 cut(s) 709
BssNI GRCGYC 1 cut(s) 618
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 4 cut(s) 80, 204, 226, 873
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 4 cut(s) 24, 335, 586, 834
Bst4CI ACNGT 1 cut(s) 626
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 618
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 4 cut(s) 472, 616, 810, 905
BstDEI CTNAG 3 cut(s) 199, 548, 659
BstDSI CCRYGG 1 cut(s) 873
BstENI CCTNNNNNAGG 2 cut(s) 27, 831
BstF5I GGATG 3 cut(s) 514, 566, 745
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 2 cut(s) 501, 620
BstKTI GATC 1 cut(s) 712
BstMAI GTCTC 1 cut(s) 610
BstMBI GATC 1 cut(s) 709
BstMWI GCNNNNNNNGC 8 cut(s) 53, 83, 231, 288, 327, 336, 626, 805
BstNI CCWGG 4 cut(s) 24, 335, 586, 834
BstSCI CCNGG 5 cut(s) 22, 333, 343, 584, 832
BstV1I GCAGC 3 cut(s) 97, 362, 652
BsuRI GGCC 6 cut(s) 163, 225, 333, 518, 584, 907
BtgI CCRYGG 1 cut(s) 873
BtsCI GGATG 3 cut(s) 514, 566, 745
BtsI GCAGTG 2 cut(s) 283, 289
BtsIMutI CAGTG 2 cut(s) 283, 289
BveI ACCTGC 1 cut(s) 835
Cac8I GCNNGC 4 cut(s) 472, 616, 810, 905
CfoI GCGC 2 cut(s) 501, 620
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 223, 582, 836
Csp6I GTAC 2 cut(s) 4, 67
CviAII CATG 2 cut(s) 437, 874
CviQI GTAC 2 cut(s) 4, 67
DdeI CTNAG 3 cut(s) 199, 548, 659
DinI GGCGCC 1 cut(s) 619
DpnI GATC 1 cut(s) 711
DpnII GATC 1 cut(s) 709
EaeI YGGCCR 2 cut(s) 331, 905
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
EciI GGCGGA 3 cut(s) 306, 762, 774
Eco130I CCWWGG 4 cut(s) 80, 204, 226, 873
Eco147I AGGCCT 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 873
Eco47I GGWCC 1 cut(s) 836
EcoNI CCTNNNNNAGG 2 cut(s) 27, 831
EcoO109I RGGNCCY 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 4 cut(s) 22, 333, 584, 832
EcoT14I CCWWGG 4 cut(s) 80, 204, 226, 873
EcoT38I GRGCYC 1 cut(s) 873
EgeI GGCGCC 1 cut(s) 619
EheI GGCGCC 1 cut(s) 619
ErhI CCWWGG 4 cut(s) 80, 204, 226, 873
FaeI CATG 2 cut(s) 440, 877
FaiI YATR 9 cut(s) 357, 425, 438, 526, 528, 569, 576, 875, 892
FaqI GGGAC 2 cut(s) 142, 546
FatI CATG 2 cut(s) 436, 873
FauI CCCGC 2 cut(s) 314, 407
FauNDI CATATG 1 cut(s) 526
Fnu4HI GCNGC 8 cut(s) 48, 108, 111, 235, 328, 331, 351, 666
FokI GGATG 3 cut(s) 521, 573, 752
FriOI GRGCYC 1 cut(s) 873
Fsp4HI GCNGC 8 cut(s) 48, 108, 111, 235, 328, 331, 351, 666
FspBI CTAG 3 cut(s) 81, 486, 701
GlaI GCGC 2 cut(s) 500, 619
GluI GCNGC 8 cut(s) 48, 108, 111, 235, 328, 331, 351, 666
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 45
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 6 cut(s) 163, 225, 333, 518, 584, 907
HapII CCGG 2 cut(s) 270, 345
HhaI GCGC 2 cut(s) 501, 620
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 2 cut(s) 440, 877
Hin6I GCGC 2 cut(s) 499, 618
HinP1I GCGC 2 cut(s) 499, 618
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 1 cut(s) 879
HpaII CCGG 2 cut(s) 270, 345
HphI GGTGA 2 cut(s) 146, 719
Hpy188I TCNGA 3 cut(s) 64, 660, 714
Hpy188III TCNNGA 3 cut(s) 120, 547, 558
Hpy99I CGWCG 1 cut(s) 799
HpyAV CCTTC 4 cut(s) 23, 179, 214, 727
HpyCH4III ACNGT 1 cut(s) 626
HpyCH4IV ACGT 1 cut(s) 535
HpyCH4V TGCA 6 cut(s) 350, 470, 596, 629, 679, 830
HpyF10VI GCNNNNNNNGC 8 cut(s) 53, 83, 231, 288, 327, 336, 626, 805
HpyF3I CTNAG 3 cut(s) 199, 548, 659
HpySE526I ACGT 1 cut(s) 535
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 2 cut(s) 440, 877
HspAI GCGC 2 cut(s) 499, 618
KasI GGCGCC 1 cut(s) 617
Kzo9I GATC 1 cut(s) 709
LmnI GCTCC 4 cut(s) 61, 82, 91, 417
Lsp1109I GCAGC 3 cut(s) 97, 362, 652
LweI GCATC 3 cut(s) 43, 551, 730
MaeI CTAG 3 cut(s) 81, 486, 701
MaeII ACGT 1 cut(s) 535
MalI GATC 1 cut(s) 711
MboI GATC 1 cut(s) 709
MboII GAAGA 5 cut(s) 26, 157, 184, 375, 742
MhlI GDGCHC 2 cut(s) 96, 873
MluCI AATT 2 cut(s) 630, 654
Mly113I GGCGCC 1 cut(s) 618
MseI TTAA 2 cut(s) 492, 648
MspI CCGG 2 cut(s) 270, 345
MspR9I CCNGG 5 cut(s) 24, 335, 345, 586, 834
MvaI CCWGG 4 cut(s) 24, 335, 586, 834
MwoI GCNNNNNNNGC 8 cut(s) 53, 83, 231, 288, 327, 336, 626, 805
NarI GGCGCC 1 cut(s) 618
NciI CCSGG 1 cut(s) 345
NcoI CCATGG 1 cut(s) 873
NdeI CATATG 1 cut(s) 526
NdeII GATC 1 cut(s) 709
NlaIII CATG 2 cut(s) 440, 877
NlaIV GGNNCC 4 cut(s) 78, 268, 583, 619
NspV TTCGAA 1 cut(s) 540
PaqCI CACCTGC 1 cut(s) 835
PceI AGGCCT 1 cut(s) 163
PfeI GAWTC 1 cut(s) 879
PkrI GCNGC 8 cut(s) 49, 109, 112, 236, 329, 332, 352, 667
PluTI GGCGCC 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 648
PsiI TTATAA 1 cut(s) 357
Psp6I CCWGG 4 cut(s) 22, 333, 584, 832
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 4 cut(s) 22, 333, 584, 832
PspN4I GGNNCC 4 cut(s) 78, 268, 583, 619
PspPI GGNCC 3 cut(s) 223, 582, 836
RsaI GTAC 2 cut(s) 5, 68
RsaNI GTAC 2 cut(s) 4, 67
SaqAI TTAA 2 cut(s) 492, 648
SatI GCNGC 8 cut(s) 48, 108, 111, 235, 328, 331, 351, 666
Sau3AI GATC 1 cut(s) 709
Sau96I GGNCC 3 cut(s) 223, 582, 836
ScrFI CCNGG 5 cut(s) 24, 335, 345, 586, 834
SduI GDGCHC 2 cut(s) 96, 873
SfaNI GCATC 3 cut(s) 43, 551, 730
SfoI GGCGCC 1 cut(s) 619
SfuI TTCGAA 1 cut(s) 540
SinI GGWCC 1 cut(s) 836
Sse9I AATT 2 cut(s) 630, 654
SseBI AGGCCT 1 cut(s) 163
SspDI GGCGCC 1 cut(s) 617
SspMI CTAG 3 cut(s) 81, 486, 701
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 5 cut(s) 22, 333, 343, 584, 832
StyI CCWWGG 4 cut(s) 80, 204, 226, 873
TaaI ACNGT 1 cut(s) 626
TaiI ACGT 1 cut(s) 538
TaqI TCGA 1 cut(s) 540
TasI AATT 2 cut(s) 630, 654
TauI GCSGC 5 cut(s) 50, 110, 237, 330, 333
TfiI GAWTC 1 cut(s) 879
Tru1I TTAA 2 cut(s) 492, 648
Tru9I TTAA 2 cut(s) 492, 648
TscAI CASTG 2 cut(s) 283, 289
TseI GCWGC 3 cut(s) 110, 350, 665
TspDTI ATGAA 1 cut(s) 453
TspRI CASTG 2 cut(s) 283, 289
VpaK11BI GGWCC 1 cut(s) 836
VspI ATTAAT 1 cut(s) 648
XagI CCTNNNNNAGG 2 cut(s) 27, 831
XapI RAATTY 1 cut(s) 654
XmaJI CCTAGG 1 cut(s) 80
XspI CTAG 3 cut(s) 81, 486, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.