Rh1DG224400

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010554
Physical Location & Seq
Reverse (-)
43494687 .. 43499847
5161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG224400.1

Sequence Viewer

Length: 537 bp
ATGAACAGGGAAGCTGATGTCGAGGACGACGGCCTTGGCGCTATCCATGATGATGGTGGCCATGTGGTCCAGAGGCTTCGAAGATGGAAAGCCATATCTGAACTGTACCCTCTGCATAACCAGCCGCGGTTAGGCAATCCCGGGGCAGCTTACAATGGAAGAAGCTTTATGCCCCACATCATATCTGTCAATGCTGGGGAGGGGCGTTTTGAGATCCTGAGCCTGTCTGGATGCTTTTTGCCCCAAGGAGACAGGGCCTGGATTGGTGGGATGAACATCTCTATGGCACGCGCTGACCGTCAAGTTTTAGGGGGAGGGGTTAATGGATTTCTGAGAGCTGCCGCTCCTGTGCAAGTTGTGGTGGGTAGTTTCCCAGCTGGTGATCAGAAAAACCAGAAAGGAAAAGGTTCAAAAAAGTCGAAGGCTGAACCATCTAACACTTCGCCGCCGCCGTCACCAGCACCAGCGCCAGCACAAGCACCAGCACCAGAACCAGAACCAGAACCCACACCCTTACCCTTTACCGTGGGATTCTAG

Protein Analysis

178

Amino Acids

18.79

Weight (kDa)

7.93

Isoelectric Point (pI)

64.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 66 - 125 2.9e-10 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 344
AccII CGCG 2 cut(s) 127, 291
AciI CCGC 5 cut(s) 125, 127, 342, 446, 449
AclWI GGATC 1 cut(s) 208
AcoI YGGCCR 1 cut(s) 58
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 1 cut(s) 411
AjnI CCWGG 1 cut(s) 257
AluBI AGCT 5 cut(s) 14, 149, 165, 338, 377
AluI AGCT 5 cut(s) 14, 149, 165, 338, 377
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 208
AlwNI CAGNNNCTG 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 3 cut(s) 31, 58, 255
ApeKI GCWGC 2 cut(s) 146, 338
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 3 cut(s) 41, 293, 469
AspS9I GGNCC 2 cut(s) 67, 255
AsuC2I CCSGG 2 cut(s) 141, 142
AsuHPI GGTGA 2 cut(s) 392, 447
AsuII TTCGAA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 67
BalI TGGCCA 1 cut(s) 60
BbvI GCAGC 2 cut(s) 158, 325
BccI CCATC 3 cut(s) 47, 78, 439
BceAI ACGGC 2 cut(s) 46, 436
BciT130I CCWGG 1 cut(s) 259
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 2 cut(s) 141, 142
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 1 cut(s) 535
BfoI RGCGCY 2 cut(s) 42, 470
BisI GCNGC 6 cut(s) 125, 147, 339, 342, 446, 449
BlsI GCNGC 6 cut(s) 126, 148, 340, 343, 447, 450
Bme1390I CCNGG 3 cut(s) 141, 142, 259
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 2 cut(s) 67, 255
BmrFI CCNGG 3 cut(s) 141, 142, 259
BmsI GCATC 1 cut(s) 221
Bpu10I CCTNAGC 1 cut(s) 218
Bpu14I TTCGAA 1 cut(s) 79
BpuMI CCSGG 2 cut(s) 141, 142
BsaBI GATNNNNATC 1 cut(s) 275
BsaJI CCNNGG 6 cut(s) 34, 125, 140, 141, 244, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse8I GATNNNNATC 1 cut(s) 275
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 6 cut(s) 34, 125, 140, 141, 244, 525
BseGI GGATG 2 cut(s) 236, 276
BseJI GATNNNNATC 1 cut(s) 275
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 2 cut(s) 209, 323
BseXI GCAGC 2 cut(s) 158, 325
BseYI CCCAGC 2 cut(s) 194, 373
Bsh1236I CGCG 2 cut(s) 127, 291
BshFI GGCC 3 cut(s) 33, 60, 257
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 243
BsnI GGCC 3 cut(s) 33, 60, 257
BsoBI CYCGRG 1 cut(s) 140
Bsp119I TTCGAA 1 cut(s) 79
Bsp143I GATC 2 cut(s) 213, 382
BspACI CCGC 5 cut(s) 125, 127, 342, 446, 449
BspANI GGCC 3 cut(s) 33, 60, 257
BspCNI CTCAG 2 cut(s) 210, 324
BspFNI CGCG 2 cut(s) 127, 291
BspPI GGATC 1 cut(s) 208
BspT104I TTCGAA 1 cut(s) 79
BsrBI CCGCTC 1 cut(s) 344
BssECI CCNNGG 6 cut(s) 34, 125, 140, 141, 244, 525
BssMI GATC 2 cut(s) 213, 382
BssT1I CCWWGG 2 cut(s) 34, 244
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 3 cut(s) 105, 299, 526
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 289, 471
BstDEI CTNAG 2 cut(s) 218, 332
BstDSI CCRYGG 2 cut(s) 125, 525
BstF5I GGATG 2 cut(s) 236, 276
BstFNI CGCG 2 cut(s) 127, 291
BstH2I RGCGCY 2 cut(s) 42, 470
BstHHI GCGC 3 cut(s) 41, 293, 469
BstKTI GATC 2 cut(s) 216, 385
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 2 cut(s) 213, 382
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNI CCWGG 1 cut(s) 259
BstSCI CCNGG 3 cut(s) 139, 140, 257
BstUI CGCG 2 cut(s) 127, 291
BstV1I GCAGC 2 cut(s) 158, 325
BstX2I RGATCY 1 cut(s) 213
BstXI CCANNNNNNTGG 1 cut(s) 53
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 3 cut(s) 33, 60, 257
BtgI CCRYGG 2 cut(s) 125, 525
BtsCI GGATG 2 cut(s) 236, 276
Cac8I GCNNGC 2 cut(s) 289, 471
CaiI CAGNNNCTG 1 cut(s) 258
CfoI GCGC 3 cut(s) 41, 293, 469
Cfr13I GGNCC 2 cut(s) 67, 255
Cfr42I CCGCGG 1 cut(s) 128
Cfr9I CCCGGG 1 cut(s) 140
Csp6I GTAC 1 cut(s) 106
CviAII CATG 2 cut(s) 47, 62
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 2 cut(s) 218, 332
DpnI GATC 2 cut(s) 215, 384
DpnII GATC 2 cut(s) 213, 382
EaeI YGGCCR 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 34, 244
Eco47I GGWCC 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 34, 244
ErhI CCWWGG 2 cut(s) 34, 244
FaeI CATG 2 cut(s) 50, 65
FaiI YATR 7 cut(s) 48, 63, 95, 117, 170, 182, 284
FatI CATG 2 cut(s) 46, 61
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 6 cut(s) 125, 147, 339, 342, 446, 449
FokI GGATG 2 cut(s) 243, 283
Fsp4HI GCNGC 6 cut(s) 125, 147, 339, 342, 446, 449
FspBI CTAG 1 cut(s) 535
GlaI GCGC 3 cut(s) 40, 292, 468
GluI GCNGC 6 cut(s) 125, 147, 339, 342, 446, 449
GsaI CCCAGC 2 cut(s) 198, 377
HaeII RGCGCY 2 cut(s) 42, 470
HaeIII GGCC 3 cut(s) 33, 60, 257
HapII CCGG 1 cut(s) 141
HhaI GCGC 3 cut(s) 41, 293, 469
Hin1II CATG 2 cut(s) 50, 65
Hin6I GCGC 3 cut(s) 39, 291, 467
HinP1I GCGC 3 cut(s) 39, 291, 467
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 1 cut(s) 531
HpaII CCGG 1 cut(s) 141
HphI GGTGA 2 cut(s) 392, 447
Hpy188I TCNGA 3 cut(s) 100, 333, 387
Hpy188III TCNNGA 3 cut(s) 70, 217, 228
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 3 cut(s) 105, 299, 526
HpyCH4V TGCA 2 cut(s) 115, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpyF3I CTNAG 2 cut(s) 218, 332
Hsp92II CATG 2 cut(s) 50, 65
HspAI GCGC 3 cut(s) 39, 291, 467
Ksp22I TGATCA 1 cut(s) 382
KspI CCGCGG 1 cut(s) 128
Kzo9I GATC 2 cut(s) 213, 382
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 2 cut(s) 158, 325
LweI GCATC 1 cut(s) 221
MaeI CTAG 1 cut(s) 535
MaeIII GTNAC 1 cut(s) 453
MalI GATC 2 cut(s) 215, 384
MbiI CCGCTC 1 cut(s) 344
MboI GATC 2 cut(s) 213, 382
MboII GAAGA 2 cut(s) 93, 171
MflI RGATCY 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 60
MluNI TGGCCA 1 cut(s) 60
MnlI CCTC 5 cut(s) 16, 66, 120, 193, 308
Mox20I TGGCCA 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 406
MscI TGGCCA 1 cut(s) 60
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 2 cut(s) 51, 281
Msp20I TGGCCA 1 cut(s) 60
MspA1I CMGCKG 2 cut(s) 127, 377
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 3 cut(s) 141, 142, 259
MvaI CCWGG 1 cut(s) 259
MvnI CGCG 2 cut(s) 127, 291
MwoI GCNNNNNNNGC 1 cut(s) 121
NciI CCSGG 2 cut(s) 141, 142
NdeII GATC 2 cut(s) 213, 382
NlaIII CATG 2 cut(s) 50, 65
NmuCI GTSAC 1 cut(s) 453
NspV TTCGAA 1 cut(s) 79
PcsI WCGNNNNNNNCGW 2 cut(s) 295, 449
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 531
PkrI GCNGC 6 cut(s) 126, 148, 340, 343, 447, 450
Psp6I CCWGG 1 cut(s) 257
PspFI CCCAGC 2 cut(s) 194, 373
PspGI CCWGG 1 cut(s) 257
PspPI GGNCC 2 cut(s) 67, 255
PstNI CAGNNNCTG 1 cut(s) 258
PsuI RGATCY 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
RseI CAYNNNNRTG 2 cut(s) 51, 281
SacII CCGCGG 1 cut(s) 128
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 6 cut(s) 125, 147, 339, 342, 446, 449
Sau3AI GATC 2 cut(s) 213, 382
Sau96I GGNCC 2 cut(s) 67, 255
ScrFI CCNGG 3 cut(s) 141, 142, 259
SetI ASST 6 cut(s) 16, 151, 167, 340, 379, 409
SfaNI GCATC 1 cut(s) 221
Sfr303I CCGCGG 1 cut(s) 128
SfuI TTCGAA 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 128
SinI GGWCC 1 cut(s) 67
SmaI CCCGGG 1 cut(s) 142
SmiMI CAYNNNNRTG 2 cut(s) 51, 281
SsiI CCGC 5 cut(s) 125, 127, 342, 446, 449
SspMI CTAG 1 cut(s) 535
StyD4I CCNGG 3 cut(s) 139, 140, 257
StyI CCWWGG 2 cut(s) 34, 244
TaaI ACNGT 3 cut(s) 105, 299, 526
TaqI TCGA 3 cut(s) 21, 79, 419
TauI GCSGC 4 cut(s) 127, 344, 448, 451
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TseFI GTSAC 1 cut(s) 453
TseI GCWGC 2 cut(s) 146, 338
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 2 cut(s) 17, 287
TspMI CCCGGG 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 67
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XmaI CCCGGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 406
XspI CTAG 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.