Rroxscaffold_5G00337090

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010554
Physical Location & Seq
Reverse (-)
5115017 .. 5117195
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00337090.1

Sequence Viewer

Length: 939 bp
ATGTACGCCAGAGAAGAACTGCCTGGAGAAGGGGTTGAAGTGATTGCGGCTGATGCTCCCTCGGAGTACCAGATGGCTCCTAGGGCGGTGGAGCACAAGGACAAGGCGGCTGCTGGTTCAGGAGAGGGGACGGGTGAGCCAAAGAAGAGAGGGAGAGGGAGGCCTCCACAGAAGAATGTGGGGGAAGGGCAGTCCTCCTCAGCCAAGGGGAGAAAGGAGAAGGGCCAAGGAAGCGGCGGTGGGGGTGGAGGAGGTACAAGTGGAGGTGCCGGTGGCAGTGGCAGTGGCGGTGGCGGTGGGGGCGGGGGCGGCGGTGGGGGTGATAATTGTAATATTGTAGGCAATCCCGGTGCAGCTTATAATGGAAGGAGCTTCACGCCCCACCTCATCTCTGTCAATGTTGGGGAGAATGTCTGCGGGAGCGTTATGAGATATTTCCATGAAAGACCACGAGTAATCTGCGTGATCTCTGCAAGTGGAGTAATCTCTAGTGTTAATCTGCGCCAACAGGATGACGAGGCCATCACATATGAGGGACGTTTCGAAATCCTGAGCTTGTCTGGATGCCTTATGCCCCATAACGGGGCCTGGCGTGGTGCAATGAGTGTCTCTCTGGCAGGCGGCAACGGTCTAATTTTAGGGGGAGGCATTAATGGAATTCTGAGAGCTGCCACTCCTGTGCAAGTTGTGGTAGGTAGTTTCCTAGCTGGTGATCCGAAAGGAAAGGGTAAGAAGAAGGCGAATGCTGAACCACCTGATACTGAGCCTCCACCTCCGCCTCCGCCGTCGCCTCCACCACCGCCGTCTCCTCCGCCGCCCGCACCAGAACCGCCACCTGCACCAGGACCACCACCACCAGCAGCAGCAGCCCCACCACCACAAGAGCCCATGGGATTCTGGGGGATATACGATAATAGCTGGCCGAGAATGGAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

312

Amino Acids

31.28

Weight (kDa)

6.91

Isoelectric Point (pI)

57.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 127 - 234 1.6e-19 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AarI CACCTGC 1 cut(s) 844
Acc36I ACCTGC 1 cut(s) 844
AccB1I GGYRCC 1 cut(s) 266
AclWI GGATC 1 cut(s) 707
AcoI YGGCCR 1 cut(s) 920
AcsI RAATTY 1 cut(s) 657
AfaI GTAC 3 cut(s) 5, 68, 256
AfiI CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 3 cut(s) 22, 587, 841
AleI CACNNNNGTG 1 cut(s) 677
AluBI AGCT 6 cut(s) 356, 372, 555, 668, 707, 918
AluI AGCT 6 cut(s) 356, 372, 555, 668, 707, 918
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 613, 810
AlwI GGATC 1 cut(s) 707
AoxI GGCC 5 cut(s) 161, 223, 519, 585, 920
ApeKI GCWGC 6 cut(s) 110, 353, 668, 860, 863, 866
ApoI RAATTY 1 cut(s) 657
AseI ATTAAT 1 cut(s) 651
AspA2I CCTAGG 1 cut(s) 80
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 3 cut(s) 223, 585, 845
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 3 cut(s) 146, 332, 722
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 1 cut(s) 845
AvrII CCTAGG 1 cut(s) 80
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 888
BauI CACGAG 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 199
BbvI GCAGC 6 cut(s) 97, 365, 655, 872, 875, 878
BccI CCATC 2 cut(s) 67, 530
BceAI ACGGC 2 cut(s) 769, 787
BcgI CGANNNNNNTGC 2 cut(s) 441, 475
BciT130I CCWGG 3 cut(s) 24, 589, 843
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 2 cut(s) 613, 810
BfaI CTAG 3 cut(s) 81, 489, 704
BfuAI ACCTGC 1 cut(s) 844
BlnI CCTAGG 1 cut(s) 80
Bme1390I CCNGG 4 cut(s) 24, 348, 589, 843
Bme18I GGWCC 1 cut(s) 845
BmgT120I GGNCC 3 cut(s) 223, 585, 845
BmiI GGNNCC 3 cut(s) 78, 268, 586
BmrFI CCNGG 4 cut(s) 24, 348, 589, 843
BmsI GCATC 2 cut(s) 43, 554
BplI GAGNNNNNCTC 2 cut(s) 595, 627
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 2 cut(s) 199, 551
Bpu14I TTCGAA 1 cut(s) 543
BpuMI CCSGG 1 cut(s) 348
BsaJI CCNNGG 5 cut(s) 60, 80, 204, 226, 888
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 751, 781
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
Bse118I RCCGGY 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 3 cut(s) 24, 589, 843
BseDI CCNNGG 5 cut(s) 60, 80, 204, 226, 888
BseGI GGATG 2 cut(s) 517, 569
BseLI CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 4 cut(s) 213, 542, 653, 753
BseRI GAGGAG 3 cut(s) 187, 264, 798
BseXI GCAGC 6 cut(s) 97, 365, 655, 872, 875, 878
BsgI GTGCAG 2 cut(s) 372, 822
BshFI GGCC 5 cut(s) 163, 225, 521, 587, 922
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 270, 348
BslFI GGGAC 2 cut(s) 142, 549
BslI CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
BsmAI GTCTC 2 cut(s) 613, 810
BsmBI CGTCTC 1 cut(s) 810
BsmFI GGGAC 2 cut(s) 142, 549
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 5 cut(s) 163, 225, 521, 587, 922
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 2 cut(s) 96, 888
Bsp143I GATC 2 cut(s) 465, 712
Bsp19I CCATGG 1 cut(s) 888
BspANI GGCC 5 cut(s) 163, 225, 521, 587, 922
BspCNI CTCAG 4 cut(s) 212, 543, 654, 754
BspLI GGNNCC 3 cut(s) 78, 268, 586
BspMI ACCTGC 1 cut(s) 844
BspPI GGATC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 5 cut(s) 60, 80, 204, 226, 888
BssMI GATC 2 cut(s) 465, 712
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 4 cut(s) 80, 204, 226, 888
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 3 cut(s) 24, 589, 843
Bst4CI ACNGT 1 cut(s) 629
Bst6I CTCTTC 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 3 cut(s) 619, 819, 920
BstDEI CTNAG 4 cut(s) 199, 551, 662, 762
BstDSI CCRYGG 1 cut(s) 888
BstENI CCTNNNNNAGG 2 cut(s) 27, 840
BstF5I GGATG 2 cut(s) 517, 569
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 2 cut(s) 468, 715
BstMAI GTCTC 2 cut(s) 613, 810
BstMBI GATC 2 cut(s) 465, 712
BstMWI GCNNNNNNNGC 6 cut(s) 53, 83, 231, 300, 309, 866
BstNI CCWGG 3 cut(s) 24, 589, 843
BstSCI CCNGG 4 cut(s) 22, 346, 587, 841
BstV1I GCAGC 6 cut(s) 97, 365, 655, 872, 875, 878
BsuRI GGCC 5 cut(s) 163, 225, 521, 587, 922
BtgI CCRYGG 1 cut(s) 888
BtsCI GGATG 2 cut(s) 517, 569
BtsI GCAGTG 2 cut(s) 283, 289
BtsIMutI CAGTG 2 cut(s) 283, 289
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 3 cut(s) 619, 819, 920
CfoI GCGC 1 cut(s) 504
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 223, 585, 845
Csp6I GTAC 3 cut(s) 4, 67, 255
CviAII CATG 2 cut(s) 440, 889
CviQI GTAC 3 cut(s) 4, 67, 255
DdeI CTNAG 4 cut(s) 199, 551, 662, 762
DpnI GATC 2 cut(s) 467, 714
DpnII GATC 2 cut(s) 465, 712
EaeI YGGCCR 1 cut(s) 920
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
EciI GGCGGA 3 cut(s) 765, 771, 801
Eco130I CCWWGG 4 cut(s) 80, 204, 226, 888
Eco147I AGGCCT 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 888
Eco47I GGWCC 1 cut(s) 845
EcoNI CCTNNNNNAGG 2 cut(s) 27, 840
EcoO109I RGGNCCY 1 cut(s) 585
EcoRI GAATTC 1 cut(s) 657
EcoRII CCWGG 3 cut(s) 22, 587, 841
EcoT14I CCWWGG 4 cut(s) 80, 204, 226, 888
EcoT38I GRGCYC 1 cut(s) 888
ErhI CCWWGG 4 cut(s) 80, 204, 226, 888
Esp3I CGTCTC 1 cut(s) 810
FaeI CATG 2 cut(s) 443, 892
FaiI YATR 9 cut(s) 360, 428, 441, 529, 531, 572, 579, 890, 907
FaqI GGGAC 2 cut(s) 142, 549
FatI CATG 2 cut(s) 439, 888
FauI CCCGC 3 cut(s) 296, 410, 826
FauNDI CATATG 1 cut(s) 529
FokI GGATG 2 cut(s) 524, 576
FriOI GRGCYC 1 cut(s) 888
FspBI CTAG 3 cut(s) 81, 489, 704
GlaI GCGC 1 cut(s) 503
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 5 cut(s) 163, 225, 521, 587, 922
HapII CCGG 2 cut(s) 270, 348
HhaI GCGC 1 cut(s) 504
Hin1II CATG 2 cut(s) 443, 892
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HinfI GANTC 1 cut(s) 894
HpaII CCGG 2 cut(s) 270, 348
HphI GGTGA 3 cut(s) 146, 332, 722
Hpy188I TCNGA 3 cut(s) 64, 663, 717
Hpy188III TCNNGA 3 cut(s) 120, 550, 561
Hpy99I CGWCG 1 cut(s) 790
HpyAV CCTTC 5 cut(s) 23, 179, 214, 360, 730
HpyCH4III ACNGT 1 cut(s) 629
HpyCH4IV ACGT 1 cut(s) 538
HpyCH4V TGCA 5 cut(s) 353, 473, 599, 682, 839
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 83, 231, 300, 309, 866
HpyF3I CTNAG 4 cut(s) 199, 551, 662, 762
HpySE526I ACGT 1 cut(s) 538
Hsp92II CATG 2 cut(s) 443, 892
HspAI GCGC 1 cut(s) 502
Kzo9I GATC 2 cut(s) 465, 712
LmnI GCTCC 5 cut(s) 61, 82, 91, 369, 420
Lsp1109I GCAGC 6 cut(s) 97, 365, 655, 872, 875, 878
LweI GCATC 2 cut(s) 43, 554
MaeI CTAG 3 cut(s) 81, 489, 704
MaeII ACGT 1 cut(s) 538
MalI GATC 2 cut(s) 467, 714
MboI GATC 2 cut(s) 465, 712
MboII GAAGA 4 cut(s) 26, 157, 184, 745
MhlI GDGCHC 2 cut(s) 96, 888
MluCI AATT 3 cut(s) 325, 633, 657
MseI TTAA 2 cut(s) 495, 651
MslI CAYNNNNRTG 1 cut(s) 677
MspI CCGG 2 cut(s) 270, 348
MspR9I CCNGG 4 cut(s) 24, 348, 589, 843
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 3 cut(s) 24, 589, 843
MwoI GCNNNNNNNGC 6 cut(s) 53, 83, 231, 300, 309, 866
NciI CCSGG 1 cut(s) 348
NcoI CCATGG 1 cut(s) 888
NdeI CATATG 1 cut(s) 529
NdeII GATC 2 cut(s) 465, 712
NlaIII CATG 2 cut(s) 443, 892
NlaIV GGNNCC 3 cut(s) 78, 268, 586
NspV TTCGAA 1 cut(s) 543
OliI CACNNNNGTG 1 cut(s) 677
PaqCI CACCTGC 1 cut(s) 844
PceI AGGCCT 1 cut(s) 163
PctI GAATGC 1 cut(s) 748
PfeI GAWTC 1 cut(s) 894
PshBI ATTAAT 1 cut(s) 651
PsiI TTATAA 1 cut(s) 360
Psp6I CCWGG 3 cut(s) 22, 587, 841
PspGI CCWGG 3 cut(s) 22, 587, 841
PspN4I GGNNCC 3 cut(s) 78, 268, 586
PspPI GGNCC 3 cut(s) 223, 585, 845
RsaI GTAC 3 cut(s) 5, 68, 256
RsaNI GTAC 3 cut(s) 4, 67, 255
RseI CAYNNNNRTG 1 cut(s) 677
SaqAI TTAA 2 cut(s) 495, 651
Sau3AI GATC 2 cut(s) 465, 712
Sau96I GGNCC 3 cut(s) 223, 585, 845
ScrFI CCNGG 4 cut(s) 24, 348, 589, 843
SduI GDGCHC 2 cut(s) 96, 888
SfaNI GCATC 2 cut(s) 43, 554
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 677
Sse9I AATT 3 cut(s) 325, 633, 657
SseBI AGGCCT 1 cut(s) 163
SspI AATATT 1 cut(s) 334
SspMI CTAG 3 cut(s) 81, 489, 704
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 4 cut(s) 22, 346, 587, 841
StyI CCWWGG 4 cut(s) 80, 204, 226, 888
TaaI ACNGT 1 cut(s) 629
TaiI ACGT 1 cut(s) 541
TaqI TCGA 1 cut(s) 543
TasI AATT 3 cut(s) 325, 633, 657
TauI GCSGC 6 cut(s) 50, 110, 237, 312, 624, 817
TfiI GAWTC 1 cut(s) 894
Tru1I TTAA 2 cut(s) 495, 651
Tru9I TTAA 2 cut(s) 495, 651
TscAI CASTG 2 cut(s) 283, 289
TseI GCWGC 6 cut(s) 110, 353, 668, 860, 863, 866
TspDTI ATGAA 1 cut(s) 456
TspRI CASTG 2 cut(s) 283, 289
VpaK11BI GGWCC 1 cut(s) 845
VspI ATTAAT 1 cut(s) 651
XagI CCTNNNNNAGG 2 cut(s) 27, 840
XapI RAATTY 1 cut(s) 657
XmaJI CCTAGG 1 cut(s) 80
XspI CTAG 3 cut(s) 81, 489, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.