MD03G1133200.v1.1

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010554
Physical Location & Seq
Forward (+)
13279088 .. 13280317
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1133200.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGCTAGAGTAATTCTTTCTTCCTTATTTTCTCCCTTCATGCAGGGTCCATTTCAGATTCTCTCTCTATCTGGAACATTTGTATATGGTTCAACGCGAAACCCACTTGAAAAAAGTTGGATGATAAACGTTTTGCTGGCAAATCATGATGGGAAGGCTTTCGGTGGTTCTGTAGCCGGTTTTATGATAGCTGCTGAGCCTGTTCAAATCGTTGTTGGAAGTTTTGAGCAAGGTACCAGAAAGGAAAGGAAAAAGAGCCGCCGGGCCAGATCTTCTTACCCATCGAGGGTGCCAGGAAATGGATCAGGTCATCTAATTGGTGCAAACAGCATCAATCCTGCTTCTCTACCGAGTTTTGGTCAGAATGTAAATCCTGCTCCTCTATCAGGTTTTGGTCAGAATGTTAACCCTGCTTCTCCACCGGATTTTGGCCAGAATGTTAACCCTGCTTCTCCACCGGGTTTTAATCTGAAATTCAATCCTGCTTCACTACCGGGTTTTGGTCAGAATGTCCCTCAGTCAATGCTGGGTCCAGGAACATCTATTGATTTCATCCCTTTTGTCTATGCTGAGCTTTTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

195

Amino Acids

20.51

Weight (kDa)

10.08

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 13 - 76 2.1e-08 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 2 cut(s) 233, 289
AccB7I CCANNNNNTGG 1 cut(s) 299
AccII CGCG 1 cut(s) 97
AciI CCGC 1 cut(s) 259
AclI AACGTT 1 cut(s) 129
AclWI GGATC 1 cut(s) 310
AcoI YGGCCR 1 cut(s) 430
AcsI RAATTY 1 cut(s) 473
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 6 cut(s) 286, 299, 356, 386, 428, 500
AgsI TTSAA 4 cut(s) 93, 110, 206, 478
AjnI CCWGG 2 cut(s) 292, 532
AluBI AGCT 2 cut(s) 191, 574
AluI AGCT 2 cut(s) 191, 574
AlwI GGATC 1 cut(s) 310
AoxI GGCC 2 cut(s) 264, 430
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 473
Asp700I GAANNNNTTC 1 cut(s) 158
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 3 cut(s) 47, 264, 530
AsuC2I CCSGG 3 cut(s) 263, 459, 495
AvaII GGWCC 2 cut(s) 47, 530
BalI TGGCCA 1 cut(s) 432
BanI GGYRCC 2 cut(s) 233, 289
BbvI GCAGC 1 cut(s) 178
BccI CCATC 2 cut(s) 143, 289
BciT130I CCWGG 2 cut(s) 294, 534
BcnI CCSGG 3 cut(s) 263, 459, 495
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 171
BglII AGATCT 1 cut(s) 269
BisI GCNGC 2 cut(s) 192, 259
BlpI GCTNAGC 2 cut(s) 195, 570
BlsI GCNGC 2 cut(s) 193, 260
Bme1390I CCNGG 5 cut(s) 263, 294, 459, 495, 534
Bme18I GGWCC 2 cut(s) 47, 530
BmgT120I GGNCC 3 cut(s) 47, 264, 530
BmiI GGNNCC 4 cut(s) 48, 235, 291, 531
BmrFI CCNGG 5 cut(s) 263, 294, 459, 495, 534
BmsI GCATC 1 cut(s) 339
Bpu1102I GCTNAGC 2 cut(s) 195, 570
BpuMI CCSGG 3 cut(s) 263, 459, 495
BsaWI WCCGGW 1 cut(s) 421
Bsc4I CCNNNNNNNGG 6 cut(s) 286, 299, 356, 386, 428, 500
Bse118I RCCGGY 1 cut(s) 176
BseBI CCWGG 2 cut(s) 294, 534
BseGI GGATG 2 cut(s) 126, 552
BseLI CCNNNNNNNGG 6 cut(s) 286, 299, 356, 386, 428, 500
BseMII CTCAG 3 cut(s) 186, 530, 561
BseRI GAGGAG 1 cut(s) 369
BseXI GCAGC 1 cut(s) 178
BseYI CCCAGC 1 cut(s) 526
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 2 cut(s) 266, 432
BshNI GGYRCC 2 cut(s) 233, 289
BsiSI CCGG 5 cut(s) 177, 262, 422, 458, 494
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 6 cut(s) 286, 299, 356, 386, 428, 500
BsmFI GGGAC 1 cut(s) 497
BsnI GGCC 2 cut(s) 266, 432
Bsp143I GATC 2 cut(s) 269, 302
Bsp1720I GCTNAGC 2 cut(s) 195, 570
BspACI CCGC 1 cut(s) 259
BspANI GGCC 2 cut(s) 266, 432
BspCNI CTCAG 3 cut(s) 187, 529, 562
BspFNI CGCG 1 cut(s) 97
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 4 cut(s) 48, 235, 291, 531
BspPI GGATC 1 cut(s) 310
BspT107I GGYRCC 2 cut(s) 233, 289
BsrFI RCCGGY 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 176
BssMI GATC 2 cut(s) 269, 302
Bst2UI CCWGG 2 cut(s) 294, 534
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 195, 516, 570
BstENI CCTNNNNNAGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 126, 552
BstFNI CGCG 1 cut(s) 97
BstKTI GATC 2 cut(s) 272, 305
BstMBI GATC 2 cut(s) 269, 302
BstNI CCWGG 2 cut(s) 294, 534
BstSCI CCNGG 5 cut(s) 261, 292, 457, 493, 532
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
BsuRI GGCC 2 cut(s) 266, 432
BtsCI GGATG 2 cut(s) 126, 552
Cac8I GCNNGC 1 cut(s) 138
CciI TCATGA 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 3 cut(s) 47, 264, 530
Csp6I GTAC 1 cut(s) 234
CviAII CATG 2 cut(s) 40, 146
CviJI RGCY 9 cut(s) 5, 158, 176, 191, 199, 258, 266, 432, 574
CviKI_1 RGCY 9 cut(s) 5, 158, 176, 191, 199, 258, 266, 432, 574
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 3 cut(s) 195, 516, 570
DpnI GATC 2 cut(s) 271, 304
DpnII GATC 2 cut(s) 269, 302
EaeI YGGCCR 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 47, 530
EcoNI CCTNNNNNAGG 1 cut(s) 384
EcoRII CCWGG 2 cut(s) 292, 532
FaeI CATG 2 cut(s) 43, 149
FaiI YATR 6 cut(s) 41, 85, 87, 147, 185, 567
FaqI GGGAC 1 cut(s) 497
FatI CATG 2 cut(s) 39, 145
Fnu4HI GCNGC 2 cut(s) 192, 259
FokI GGATG 2 cut(s) 133, 539
Fsp4HI GCNGC 2 cut(s) 192, 259
FspBI CTAG 1 cut(s) 6
GluI GCNGC 2 cut(s) 192, 259
GsaI CCCAGC 1 cut(s) 530
HaeIII GGCC 2 cut(s) 266, 432
HapII CCGG 5 cut(s) 177, 262, 422, 458, 494
Hin1II CATG 2 cut(s) 43, 149
HincII GTYRAC 2 cut(s) 406, 442
HindII GTYRAC 2 cut(s) 406, 442
HinfI GANTC 1 cut(s) 58
HpaI GTTAAC 2 cut(s) 406, 442
HpaII CCGG 5 cut(s) 177, 262, 422, 458, 494
Hpy166II GTNNAC 2 cut(s) 406, 442
Hpy188I TCNGA 5 cut(s) 57, 363, 399, 471, 507
Hpy188III TCNNGA 2 cut(s) 72, 146
Hpy8I GTNNAC 2 cut(s) 406, 442
HpyAV CCTTC 2 cut(s) 46, 148
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 2 cut(s) 43, 323
HpyF3I CTNAG 3 cut(s) 195, 516, 570
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 2 cut(s) 43, 149
KpnI GGTACC 1 cut(s) 237
KspAI GTTAAC 2 cut(s) 406, 442
Kzo9I GATC 2 cut(s) 269, 302
LmnI GCTCC 1 cut(s) 382
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 1 cut(s) 339
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 129
MalI GATC 2 cut(s) 271, 304
MboI GATC 2 cut(s) 269, 302
MboII GAAGA 2 cut(s) 12, 264
MflI RGATCY 1 cut(s) 269
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 3 cut(s) 12, 315, 473
MluNI TGGCCA 1 cut(s) 432
MmeI TCCRAC 2 cut(s) 98, 196
MnlI CCTC 3 cut(s) 279, 390, 525
Mox20I TGGCCA 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 158
MscI TGGCCA 1 cut(s) 432
MseI TTAA 3 cut(s) 405, 441, 465
Msp20I TGGCCA 1 cut(s) 432
MspI CCGG 5 cut(s) 177, 262, 422, 458, 494
MspR9I CCNGG 5 cut(s) 263, 294, 459, 495, 534
MvaI CCWGG 2 cut(s) 294, 534
MvnI CGCG 1 cut(s) 97
NciI CCSGG 3 cut(s) 263, 459, 495
NdeII GATC 2 cut(s) 269, 302
NlaIII CATG 2 cut(s) 43, 149
NlaIV GGNNCC 4 cut(s) 48, 235, 291, 531
PagI TCATGA 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 58
PflMI CCANNNNNTGG 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 2 cut(s) 193, 260
Psp1406I AACGTT 1 cut(s) 129
Psp6I CCWGG 2 cut(s) 292, 532
PspFI CCCAGC 1 cut(s) 526
PspGI CCWGG 2 cut(s) 292, 532
PspN4I GGNNCC 4 cut(s) 48, 235, 291, 531
PspPI GGNCC 3 cut(s) 47, 264, 530
PsuI RGATCY 1 cut(s) 269
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SaqAI TTAA 3 cut(s) 405, 441, 465
SatI GCNGC 2 cut(s) 192, 259
Sau3AI GATC 2 cut(s) 269, 302
Sau96I GGNCC 3 cut(s) 47, 264, 530
ScrFI CCNGG 5 cut(s) 263, 294, 459, 495, 534
SetI ASST 7 cut(s) 132, 193, 235, 310, 391, 576, 584
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 2 cut(s) 47, 530
Sse9I AATT 3 cut(s) 12, 315, 473
SsiI CCGC 1 cut(s) 259
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 5 cut(s) 261, 292, 457, 493, 532
TaiI ACGT 1 cut(s) 132
TaqI TCGA 1 cut(s) 284
TasI AATT 3 cut(s) 12, 315, 473
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 3 cut(s) 405, 441, 465
Tru9I TTAA 3 cut(s) 405, 441, 465
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 2 cut(s) 28, 541
Van91I CCANNNNNTGG 1 cut(s) 299
VpaK11BI GGWCC 2 cut(s) 47, 530
XagI CCTNNNNNAGG 1 cut(s) 384
XapI RAATTY 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.